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Anna Sagnella, October 2012 ESPERIENZE PROFESSIONALI SIGNIFICATIVE: EDUCATION October 2009 : Degree in Pharmaceutical Chemistry and Technology – Università degli Studi di Perugia. March 2010 : Master in Pharmaceutical Technologies and Regulatory Affairs - Università degli Studi di Parma, Pavia e Perugia - Consorzio TEFARCO-INNOVA January 2012 - Today : PhD student in Chemistry – Polymeric proteins as matrix for innovative formulations for targeted and controlled drug release. Almamater Studiorum, Università di Bologna , Prof. Carlo Bertucci , Dipartimento di Scienze farmaceutiche. WORK EXPERIENCE March- may 2010 : Project of research "Nanocomposites from inorganic matrix for drug delivery systems” - Dipartimento di Chim. e Tec. del Farmaco, Università degli Studi di Perugia. June - december 2010 : Stage for Design and Development, Regulatory Affairs and Quality Control of medical devices, cosmetics and OTC. Elaboration of quality manual for ISO 9001 certification. December 2010- Today : research fellow “ Study and development of systems and interfaces for bio-electronic” supported by FIRB ITALNANONET, supervised by Valentina Benfenati and Roberto Zamboni
Transcript of Presentazione standard di PowerPoint · 350 400 450 500 550 600 650 0,0 4,0x10 5 8,0x10 5 1,2x10 6...
Anna Sagnella, October 2012
ESPERIENZE PROFESSIONALI SIGNIFICATIVE:
EDUCATION
October 2009: Degree in Pharmaceutical Chemistry and Technology –
Università degli Studi di Perugia.
March 2010: Master in Pharmaceutical Technologies and Regulatory Affairs -
Università degli Studi di Parma, Pavia e Perugia - Consorzio TEFARCO-INNOVA
January 2012 - Today: PhD student in Chemistry – Polymeric proteins as matrix for innovative
formulations for targeted and controlled drug release.
Almamater Studiorum, Università di Bologna , Prof. Carlo Bertucci , Dipartimento di Scienze
farmaceutiche.
WORK EXPERIENCE
March- may 2010: Project of research "Nanocomposites from inorganic matrix for drug
delivery systems” - Dipartimento di Chim. e Tec. del Farmaco, Università degli Studi di
Perugia.
June - december 2010: Stage for Design and Development, Regulatory Affairs and
Quality Control of medical devices, cosmetics and OTC. Elaboration of quality manual for
ISO 9001 certification.
December 2010- Today: research fellow “ Study and development of systems and
interfaces for bio-electronic” supported by FIRB ITALNANONET, supervised by Valentina
Benfenati and Roberto Zamboni
BIOMANUFACTURING AND
CHARACTERIZATION OF
NATURAL FUNCTIONALIZED SILK
FIBROIN
Anna Sagnella, October 2012
SILK FIBROIN (SF) Natural protein extracted from different species of insects
BOMBYX MORI (Mulberry specie)
Anna Sagnella, October 2012
TEXILE INDUSTRY OF XIV
sec
SILK PRODUCTS: THE PAST AND THE PRESENT THE ROUTE OF SILK
HIGH FASHION SURGERY
Anna Sagnella, October 2012
THE FUTURE OF SILK
AS HIGH TECH MATERIAL IN VARIOUS FIELDS
Kaplan and Omenetto Scientific American, 2010
Tissue engineering
Photonics
Tao et al. Advanced Material, 2012 Kim et al.,Nature Materials 2010
Inorganic electronic
Pharmaceutical
Kaplan and Omenetto, Tufts University, 2012
Anna Sagnella, October 2012
No toxic solvents
Eco-friendly
Biodegradable
biocompatible
Water based
extraction,
purification and
processing
Easy to process into many material
formats
Anna Sagnella, October 2012
THE SILK FIBROIN FILM
Optically transparent
300 450 600 7500
20
40
60
80
100
T %
Wavelength (nm)
1200 1300 1400 1500 1600 1700 1800
Silk ISilk I
1235
1660
Ab
so
rba
nc
e (
a.u
.)
Wavenumber (cm-1)
15331515
Silk II
0 1 2 3 4 5 6 7 80
20
40
60
80
100
We
igh
t lo
ss
of
sil
k f
ilm
s (
%)
Dissolution time (Day)
Controlled biodegradability and water-solubility
Free standing
Flexible
Anna Sagnella, October 2012
Capelli et al.,2011
WIPO Patent Application WO/2011/026101
SILK OFET SILK OLET
SF has the features to be a novel platform
as dielectric in OFET/OLET devices
Anna Sagnella, October 2012
SF is a promising biomaterial
substrate for in vitro molecular
/functional studies targeted to
promote recovery of injured
neurons and/or to alleviate painful
disability
Neuroregenerative medicine
Benfenati Muccini et al.,
Adv Func Material, 2012
Anna Sagnella, October 2012
Low-threshold blue lasing from silk fibroin thin films
Toffanin et al.,
Appl Phis Lett, 2012
Anna Sagnella, October 2012
Functional Silk: Colored and Luminescent
Tansil et al., Adv. Mater. 2012
Intrinsically colored and luminescent silks produced through addition
of dyes into silkworms’ diet
Anna Sagnella, October 2012
Anna Sagnella, October 2012
Anna Sagnella, October 2012
Purified
Regenerated SF
water-solution
EXTRACTION AND PURIFICATION
Dialysis for
48 h in
cellulose
membrane
against
deionized water
Purification by dialisys and
centrifugation
of SF solution
Degumming:
Boiling the cocoons in
alcaline solution
(Na2CO3 0,5 M)
Solubilization of silk fibers in
LiBr concentrated solution,
60°C
Washing in deionized water of
degummed silk fibers Drying of silk fibers under a hood
Anna Sagnella, October 2012
Analyses of chemical and physical properties
SEM:
SILK FIBERS POST-DEGUMMING
OPTICAL SPECTROSCOPY:
ABSORPTION AND EMISSION PROFILES OF SF WATER-SOLUTION
300 350 400 450 500
Lu
min
es
ce
nc
e (
a.u
)
Wavelength (nm)
350 nm
305 nmexcitation=275 nm
Ab
so
rba
nc
e (
a.u
.)
250 260 270 280 290 300
Wavelength (nm)
275 nm
1H NMR: INVESTIGATION OF
AMINOACIDIC RESIDUES
Anna Sagnella, October 2012
72h 48h 24h 72h 48h 24h
20 40 60 800,00
0,01
0,02
0,03
0,04
0,05
c)
450 500 550 600 650
0,00
0,02
0,04
0,06
400 600 800
0,0
0,1
0,2
0,3
0,4
0,5
0,6
0,7
d)
Ab
so
rba
nce
Wavelength (nm)
Rose SF 72h
Rose SF 48h
Rose SF 24h
White SF
546 nm
Rose SF 72h
Rose SF 48h
Rose SF 24h
Ab
so
rba
nce
Wavelength (nm)
546 nm A
bso
rba
nce
Time (hours)
Functionalized SF water-solutions
from silkworm’s diet containing Rhodamine B
Anna Sagnella, October 2012
300 450 600 750
20
40
60
80
100
400 500 600 700 80090
95
100% T
Rose SF 72h
Rose SF 48h
Rose SF 24h
White SF
1200 1400 1600 1800
Silk II
Silk II
Silk I
Rose SF 80h
Rose SF 40h
Rose SF 20h
White SF
O.D
(a
. u
)
Wavenumber cm-1
Silk IWavelength (nm)
FUNCTIONALIZED SF FILM MADE
BY DROP-CASTING AND SLOW
DRYING METHOD
Anna Sagnella, October 2012
350 400 450 500 550 600 6500,0
4,0x105
8,0x105
1,2x106
1,6x106
550 600 650 700 7500,00
2,50x106
5,00x106
7,50x106
1,00x107
Rose SF 72h
Rose SF 48h
Rose SF 24h
White SF
Rose SF 72h
Rose SF 48h
Rose SF 24h
Lu
min
escen
ce
inte
nsity
Wavelength (nm)
Lum
inescence in
tensity
Wavelength (nm)
400 nm 415 nm
568 nm
568 nm
FLUORESCENCE SPECTRA OF SF WATER-SOLUTION
FLUORESCENCE SPECTRA OF SF FILMS
350 400 450 500 550 6000,0
0,5
1,0
550 600 650 700 750
0,0
0,5
1,0
Rose SF 72h
Rose SF 48h
Rose SF 24h
White SF
Rose SF 72h
Rose SF 48h
Rose SF 24h
Lu
min
escen
ce
inte
nsity a
.u)
Wavelength (nm) Wavelength (nm)
400 nm 415 nm
568 nm
568 nm
Lu
min
escen
ce
inte
nsity a
.u)
Excitation at 300 nm Excitation at 546 nm
Anna Sagnella, October 2012
Modifying artificial diet is an efficient method to obtained naturally and self assembled functionalized silk
fibroin solution and films
CONCLUSIONS
Modifyed artificial diet to domesticate silkworms
is an efficient method to obtain naturally
functionalized SF products
Doping diet is a promising method for:
- green fabrication of optoelectronic materials
- selecting the best uptaking silkworms species in
terms of xenobiotic concentration in the cocoons
PERSPECTIVE
Anna Sagnella, October 2012
Acknowledgement
I.S.Z.A. - SEZIONE SPECIALIZZATA
PER LA BACHICOLTURA - PADOVA
Viviana Biondo
Raffaella Capelli
Michele Muccini
Giampiero Ruani
Stefano Toffanin
Camilla Chieco
Nicola Di Virgilio
Federica Rossi
Valentina Benfenati
Assunta Pistone
Greta Varchi
Roberto Zamboni
Amy Hopkins
David Kaplan
Fiorenzo Omenetto
Silvia Capellozza
Giuliano Freddi
Tamara Posati
