Presentation Title Update on fruit fly and other insect pests

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www.nt.gov.au Presentation Title Presenter name Date of presentation Update on fruit fly and other insect pests Brian Thistleton Mango Research Meeting 29 July 2016 DEPARTMENT OF PRIMARY INDUSTRY AND FISHERIES

Transcript of Presentation Title Update on fruit fly and other insect pests

Page 1: Presentation Title Update on fruit fly and other insect pests

www.nt.gov.au

Presentation TitlePresenter name

Date of presentation

Update on fruit fly and

other insect pests

Brian Thistleton

Mango Research Meeting

29 July 2016

DEPARTMENT OF PRIMARY INDUSTRY AND FISHERIES

Page 2: Presentation Title Update on fruit fly and other insect pests

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Acknowledgements

• Entomology team

– Mary Finlay-Doney

– Natasha Burrows

– Austin McLennan

– Michael Neal

– Lanni Zhang

– Haidee Brown

• Growers who allowed access to their properties for

leafhopper monitoring and fruit fly studies

Page 3: Presentation Title Update on fruit fly and other insect pests

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Outline

• Mango leafhoppers

• Mango fruit borer

• Update on fruit fly project

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Mango leafhopper – Idioscopus nitidulus

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Mango Plant Hopper

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Sampling techniques

Counts on leaves

Sweep netting

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1

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1

• Idioscopus nitidulus (Hemiptera: Ciccadellidae)

• 8 sites, sampling fortnightly from May 2015

Mango leafhopper surveillance

Leafhoppers Mango trees

Adults

Nymphs

Eggs

Mature leaves

Bud

Flushing leaves

Flushing flowers

Panicles

Page 8: Presentation Title Update on fruit fly and other insect pests

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1

3

1

• Idioscopus nitidulus (Hemiptera: Ciccadellidae)

• 8 sites, sampling fortnightly from May 2015

Mango leafhopper surveillance

Leafhoppers Mango trees

Adults

Nymphs

Eggs

Mature leaves

BudFlushing leaves

Flushing flowersPanicles

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Site 2. Leaf hopper abundance over 12 months comparing life stage and two different survey techniques

0

20

40

60

80

100

120.

Adult sweep

net

Adult leaf

count

nymph leaf

count

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Site 1. Leaf hopper abundance over 12 months comparing life stage and two different survey techniques.

0

20

40

60

80

100

120

Adult sweep

net

Adult leaf

count

Nymph leaf

count

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Idioscopus nitidulus and I. clypealis1986 I. nitidulus discovered in

Torres Strait

1997 I. nitidulus discovered in

Cape York and NT (Darwin)

1999 I. clypealis discovered in

Cape York

2015 I. clypealis discovered in

NT (Melville Island) on citrus by

NAQS

Also on mainland – from routine

and extra monitoring Berrimah

and Virginia in April (6

specimens)

DNA - ?origin

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Mango Fruit Borer, Citripestis eutraphera (Lepidoptera: Pyralidae)

Confirmed from Cambodia by DNA analysis of a larva in 2012

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Fruit fly - Market access

1. Fruit fly populations – Darwin and Katherine, 4 years

2. Large scale collections of commercially-harvested untreated fruit

- Exposed to natural field populations of pest fruit flies

3. Cage studies – 2014 – species and variety comparisons

Exposed to extreme levels of pest fruit flies in a non-choice situation

4. Suppression studies

5. Oviposition trials

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Mangoes - Calypso (B74) and Kensington PrideFruit flies – Bactrocera jarvisi, Bactrocera tryoni

• 150+ individual fruit tested• Single sexually mature female placed in a cage with a single mango and observed for 2hrs• Measure FRUIT: Brix, DM% FLY: number of eggs in ovaries• Artificially place eggs in fruit after fly exposure

Results so far• Both species can complete development in immature fruit• Successful oviposition is not possible – unable to penetrate?

3rd December 2015

Fruit fly oviposition trials

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Analysis and write-up:

• Final report – giving results for both projects

• Papers

• Protocol

3rd December 2015

Currently

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Thank you