Presentation jayshree
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INTRODUCTION
• Insect existed at least 20 million yrs ago.
• Grain pests e.g. Rodents, Nematodes, Fungi
• Classification of pests
• Plants extracts/ botanical insecticides
• Synthetic chemical compounds
Aim Of This Project
• Toxicity Evaluation of Synthesized Chemical Compounds on Stored Grain Pests.
• To Protect Stored Grains.
Material & Methods
• Collection & Culture Of Sitophilus oryzaeLinn
• Synthesis of chemical compounds.
Observation Table
Table no 1:
2,5dihydro-7,8dimethoxy-4(3-methoxyphenyl)-2-oxo-1H-in
deno (1,2-b)pyridine-3carbonitrile.
Sr.no.
Dose
in
ppm
No.of
insects
released
Mortality
for
6hrs 12hrs 18hrs 24hrs
1 5 10 1 2 3 6
2 10 10 2 3 3 7
3 15 10 2 3 4 8
4 20 10 3 3 4 9
Table no 2:
2,5dihydro-7,8dimethoxy-4(3,4-dimethoxyphenyl)-2-
oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.
Sr.no.
Dose
in
ppm
No.of
insects
released
Mortality
for
6hrs 12hrs 18hrs 24hrs
1 5 10 2 2 2 5
2 10 10 2 3 3 6
3 15 10 3 3 3 8
4 20 10 2 3 4 9
Table no 3:
2,5dihydro-7,8dimethoxy-4(3,4,5-trimethoxyphenyl)-2-oxo-1H-
in deno (1,2-b)pyridine-3carbonitrile.
Sr.no.
Dose
in
ppm
No.of
insects
released
Mortality
for
6hrs 12hrs 18hrs 24hrs
1 5 10 1 2 3 6
2 10 10 2 3 3 8
3 15 10 2 3 4 9
4 20 10 3 3 3 9
Table no 4:
2,5dihydro-7,8dimethoxy-4(thiophen-2-yl)oxo-1H-in deno
(1,2-b)pyridine-3carbonitrile.
Sr.no.
Dose
in
ppm
No.of
insects
released
Mortality
for
6hrs 12hrs 18hrs 24hrs
1 5 10 2 2 3 6
2 10 10 2 3 3 7
3 15 10 2 3 4 9
4 20 10 3 2 3 9
Result
Chemical Time in LC50 ppmRegression eqnHeterogencityVarianceFinancial
Comp Hrs. ± S.E. Y-a+b x2 (n-1) Limit
0 5 10 15 20 A=y-bx
Comp I 24 0 60 70 80 90 1.5515 ± 0.99824.438 2.46 0.997 m1=0.0951
(101) m2=3.0075
Comp 2 24 0 50 60 80 90 1.3357 ± 0.73543.908 -2.539 0.54 m1=0.1044
(104) m2=2.567
Comp 3 24 0 60 80 90 90 1.5885 ± 0.95674.447 2.983 0.905 m1=0.1034
(105) m2=3.0736
Comp 4 24 0 60 70 90 90 1.5134 ± 0.86544.315 2.936 0.802 m1=0.0311
(106) m2=2.9957
Table No. 9 - Comparative Results
Mortality
Discussion
• There is significant increase in mortality from low to high dose of chemical compound.
• S. oryzae, R. dominica, T. castaneum shows high mortality when exposed to biogass in PVC bins (Bennett, 2003).
• Azadirachtin exhibited good efficiency against Musca nobulo L, Culex fatigans, Triboliumcastaneum (Paul et, al.1963).
• PN101, LC50=1.5515+0.9982, PN104, LC50=1.3357+0.7354, PN105,LC50=1.5134+0.8954, PN106, LC50=1.5885+0.9567.
References
• Bennett S. (2003). Stored Product insects, available on: www.thepiedpiper.co.uk/th7.htm.(Accessed 22march 2012)
• Finney D.(1971). Probit analysis Cambridge University press, London.
• Paul, C.F, R.S.Dixit and P.N. Agarwal. (1963). Evaluation of the insecticidal properties of the seed oil and leaf extract of the common Indian neem, Azadirachta indica L. Sci. Cult. 29:412-413