Green Pacific BiologicalsJunio 2012

Leandro Vetcher – CEO

lvetcher@greenpacificbio.com@lvetcher

Green Pacific Biologicals - Not for distribution

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More efficient engineering

Stable, stronger gene expression

Access to the entire cell

Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution

GPB: Plataforma de ingenieria genetica

Only company with rapid and stable eukaryotic algae nuclear genetic engineering

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GPB: Soluciones para biocombustibles

Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution

Expresión génica nuclear mas fuerte

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WT GPBGFP protein

stds (ng)Cell extract

Wild

-typ

eG

PB S

trai

ns

GFP expression by Western blotting Accumulation of GFP by confocal microscopy

Expresión génica nuclear estable

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Avg.

fluo

resc

ence

pe

r cel

l (au

)

Avg.

fluo

resc

ence

pe

r cel

l (au

)

Mas transformantes expresando transgenes

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More rapid and efficientengineering

Green Pacific Biologicals - PROPRIETARY and CONFIDENTIAL - Not for distribution

RNAi funcional- ingeniería metabólica

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Genetic RNAi using GPB platform better than traditional mutant

Starch lower Oil higher Growth normal

Membrane part+GFP Lipid droplet part+GFPUntransformed cell

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Localización subcelular de proteínaMembrane localization

Lipid droplet localization

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Secreción de aceites no-toxica Non-toxic mechanism Secreted LDs spontaneously separates, sidestepping

harvesting/extraction Works by interactions between proteins on LDs and cell membrane

or other cellular components Platform makes GPB uniquely positioned to implement oil secretion

in algae

BASE CASE:• Milk harvested by killing cow

• Majority of energy Making cow

GPB CASE:• Milk harvested through secretion and cow returned to pasture

• Majority of energy (grass) goes into making milk

• If cows are milked daily, there is no benefit to having cows that can store more than one day’s worth of milk

?

+MILK MILK

Beneficios de secreción de aceites no-toxica

Modelado Tecno-económico

• Physics: energy balanced, reasonable energy inputs•Biology: realistic growth and enzymatic rates, cell densities, PSE• Interaction between variables (e.g., cell density affects total energy captured)• Pilot and commercial capex and operational cost for PBRs and open ponds (with Tryg Lundquist, PhD at Cal Poly)

Oil productivity model + operational & capital costs

Modelado Tecno-económico: valor de GPBSecretion• Enables continuous process through recycling of cells after secretion• Most benefit through higher TAG Productivity • Elimination of several costly process steps in TAG recovery

Oil metabolic engineering• Improved redirection of light energy into TAGs • Very high oil content levels not necessary with oil secretion

Other • Identify additional bioengineering solutions• Prioritize R&D projects based on synergies and on relative impact in cost

ValidationWe can reach $50/bbl with realistic performance metrics for each of our genetic engineering solutions

Process Flow Diagram Algae Biofuel Facility

Flash Dryer

High Rate Algae Ponds

Inoculation Ponds

Settling BasinGravity

Thickener

Solar Drying Beds

Anaerobic Digester

Generator

Make-up Water

Recirculation

Algae Biomass

Algae Biomass

Algae BiomassResiduals

Solvent Based Oil-Extraction

Unrefined Oil

CH4

+

CO2

CO2Digestate

Natural Gas

Make-up Water

Blowdown

Base Case GPB Process

Work with Tryg Lundquist, Assistant Professor at Cal Poly

Algae acts a catalyst that converts CO2 and sunlight into TAGs efficiently, without being consumed in the process

0 5 10 15 20 25 30 35 40 45 50 55 600

200

400

Base Case 0%50% 90%

% Oil (w/w)Cost

per

bar

rel (

$/bb

l)

Early adoption of tecnoeconomic analysis provides: Quantification of value of biofuel solutions Guidance of R&D projects Validation

Modelado Tecno-económico