PREPFCTM Fraction Collector (Ethernet) User’s Guide
Transcript of PREPFCTM Fraction Collector (Ethernet) User’s Guide
PREPFCTM Fraction Collector (Ethernet)User’s Guide
PREPFCTM Fraction Collector (Ethernet)User’s Guide
Gilson, Inc. World Headquarters | 3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451
Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, France
www.gilson.com | [email protected] | [email protected] | [email protected]
©2008 Gilson, Inc. | LT350020-02
Table of Contents
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
1 Introduction
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2Standard Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Customer Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
2 Installation
Tray Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2Tray Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3Rack Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
3-Way Valve Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6Dispense Tube and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
System Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Plumbing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8Plumbing the Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9Plumbing Multiple Fraction Collectors . . . . . . . . . . . . . . . . . . . 2-11Cable Clip Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15Drain Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15
Rear Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16Ethernet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17Input/Output Ports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18PREPFC Contact Interface Module . . . . . . . . . . . . . . . . . . . . . . . . . 2-21Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-23Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-23
3 Operation
Start Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Run Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2Programmable Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3Remote Inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5Collection Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
4 Maintenance
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Transporting the Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Replacing Dispense Tube and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Replacing the 3-Way Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Before you Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Remove the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5Replace the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6Adjust the Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Replacing a Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
5 Troubleshooting
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Repair and Return Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Before Calling Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Non-Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Rebuilt Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5Return Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6Unit End-of-Life . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
A Replacement Parts and Accessories
B Racks
C System Organizer
Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
Attaching the Shelf Stiffeners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
Attaching the Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
Attaching the Corner Braces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
D Pocket PC Guard
Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1
Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-1
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 1-1
Introduction 1
The PREPFCTM Fraction Collector (Ethernet) is a small-footprint preparative fraction collector with a 3-way valve for flow rates up to 200 mL/min. The PREPFC has an adjustable tray platform that can be installed at four different heights and can accommodate vessel heights up to 230 mm. Up to four PREPFC Fraction Collectors can be connected to increase capacity.
The PREPFC Fraction Collector is controlled by Gilson PREPFCTM Control Software which is run on a handheld Pocket PC running Microsoft® Windows Mobile®.
Introduction 1
1-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Un
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Unpack the fraction collector and accessories carefully from the carton. Cross-check the contents against the Standard Equipment on page 1-3 and against your purchase order’s optional accessory list to verify that all parts are included and undamaged.
Do this now, even if the fraction collector will not be used immediately. Many carriers must receive concealed damage claims within seven days of delivery.
The fraction collector is delivered with most major components already assembled. Keep the original container and packing assembly in case the fraction collector must be returned to the factory.
To unpack the fraction collector:
1 Open the box and remove the cardboard insert from the top of the box.
2 There are two boxes inside, one contains the tray platform and insert (if ordered), and the other contains the accessories. Remove these boxes.
3 Grab the two handle cutouts on the cardboard insert and lift the fraction collector up and out of the box.
4 Lift the fraction collector off the cardboard insert.
Do not attempt to lift the instrument from the arm. You may carry the fraction collector using the large rectangular opening in the front of the unit.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 1-3
Introduction 1U
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Standard Equipment
Once the fraction collector and accessories are unpacked, you should have the following:
• PREPFCTM Fraction Collector (with 3-way valve and dispense tube for 1/8" OD tubing installed)
• Tray platform
• Accessory package which includes Cat 5e ethernet cable, barbed adapter (for drain tubing), terminal block connector, fuses, fuse drawers, power cords, headless nut extender tool, dispense tube for 1/16" OD tubing, and Gilson Ethernet Utility
Documentation
The following documents are included with the fraction collector:
• PREPFCTM Fraction Collector (Ethernet) Documentation CD
• IQ Procedure PREPFCTM Fraction Collector (Ethernet)
• Unpacking the PREPFCTM Fraction Collector (Ethernet)
• Declaration of Conformity
Introduction 1
1-4 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Un
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Based upon your configuration, you’ll also receive additional accessories, ordered separately.
Tray Inserts
Plumbing Packages
Multiple PREPFC Fraction Collectors
Control
To control the fraction collector you will need Gilson PREPFC Control Software.
You will also need a router, a Pocket PC, an Ethernet Compact Flash Card for Pocket PC*, and a Cat 5e ethernet cable*. They are not available for purchase from Gilson, Inc.
Optionally, purchase a Pocket PC Guard for HP iPAQ hx2100/2400-Series (part number 21052609).
* Not required if using a wireless router.
Part Number Description
17007112 Insert for four Code 20-series
17007113 Insert for two Code 200-series racks
17041950 Code 91 rack for eleven 500 mL bottles
17041951 Code 92 rack for eight 1000 mL bottles
Part Number Description
1747995 PREPFC Plumbing Package 1/8" OD
1747996 PREPFC Plumbing Package 1/16" OD
Part Number Description
17110101 PREPFC Fraction Collector (Ethernet)
17017103 PREPFC Contact Interface Module to connect up to four fraction collectors
17027114 Cable to connect from PREPFC Contact Interface Module to each fraction collector (one cable required for each fraction collector)
210526 System Organizer, PREPFC
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 1-5
Introduction 1C
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Customer Service
Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your Gilson-authorized representative. Specific contact information can be found on the Gilson website at www.gilson.com. To help us serve you quickly and efficiently, please refer to Before Calling Us on page 5-5.
Introduction 1
1-6 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Tech
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Please be aware of the following before operating the fraction collector.
Warning! Changes or modifications to the fraction collector not expressly approved by Gilson could void the factory-authorized warranty.
The fraction collector has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC commercial environment. The fraction collector generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. Operation of the fraction collector in a residential area is likely to cause harmful interference; in which case, the user will be required to correct the interference at the user’s own expense.
Shielded cables must be used with the fraction collector to ensure compliance with the Class A FCC limits.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 1-7
Introduction 1Tech
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Technical Specification Definition
3-way valve Three-port, PTFE, 114 μL internal volume, 10 μL dead volume, up to 200 mL/min
Operating pressure: 30 psi on the NO and NC port, 60 psi on the COMM port
Valve switching speed < 20 msec
Analog input (detector) ±1000 mV
Contact control Contact closure inputs for remote START, STOP, ADVANCE, and DIVERT
Two programmable outputs (which include an event mark output)
Data acquisition An input channel for analog to digital conversion
Environmental conditions Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31° C, decreasing linearly to 50% relative humidity at 40°C
Manufacturing standards Meets applicable Safety and EMC certification standards; UL, CSA, and CE certified
Maximum collection volume/tube
1000 mL (Kimax or Pyrex bottles with Code 92 rack)
Multi-cycle operation With PREPFCTM Control Software: Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
Number of racks Up to four Code 20-, Code 33X/34X-, or Code 30-series racks
Up to two Code 200-series racks
Or one Code 90-series rack
Operating modes Use PREPFCTM Control Software to collect by Time, Volume, Slope, or Level
continued (1 of 2)
Introduction 1
1-8 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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Maximum fractions Qty Capacity Gilson rack (qty)
320 13 x 100 mm (9 mL) test tubes Code 343 rack (4)
192 16 x 100 mm tubes Code 223 rack (2)
176 18 x 150 mm test tubes Code 22 rack (4)
80 25 x 200 mm test tubes Code 239 rack (2)
54 28 x 57 mm (25 mL) scintillation vials
Code 204 rack (2)
54 30 x 115 (50 mL)conical bottom tubes
Code 222 rack (2)
18 48 x 113 mm (125 mL) bottles Code 211 rack (2)
11 500 mL bottles Code 91 rack (1)
8 1 liter bottles Code 92 rack (1)
For a full list of available racks refer to Appendix B, Racks
Peak detection Use PREPFCTM Control Software to collect by Slope or Level
Physical space requirement (W x D x H)
50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in)
Power requirements Frequency: 50/60 Hz
Voltage: 100–240V, mains voltage fluctuations not to exceed 10% of the nominal voltage
Current rating: 0.50A for 100–120V
0.25A for 220–240V
Software control Stand-alone control via Ethernet using a Pocket PC running Microsoft® Windows Mobile® and PREPFCTM Control Software
Tube change time 100–250 msec, center-to-center, depending on rack type
Weight 8.6 kg (19 lbs)*
*Weight includes the tray platform
Technical Specification Definition
continued (2 of 2)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 2-1
Installation 2
This section takes you through the steps for setting up your fraction collector.
tray platform
tray insertracks
3-way valve
drain
barbed
inlet tubing
outlet tubing
tubing
adapter
spiral wrap
(from detector)
(to waste)
Installation 2
2-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Tray
Set
up Tray Setup
The fraction collector is equipped with an adjustable tray platform to hold the racks. All of the racks require the use of the tray platform.
To install the tray platform:
1 Determine the height of the rack with the installed vessels to be used to collect fractions.
2 Refer to the table below and the diagram on the left to determine the position of the tray platform.
3 Align the tray platform with the two screws on each side of the fraction collector. The notches on the top sides of the tray platform should rest below the screw on the top and the notches on bottom should rest on top of the bottom screw.
CAUTION! Before changing the height of the tray platform, remove any inserts or racks that are present.
Position Total height(rack + vessels)
A up to 80 mm
B up to 130 mm
C up to 180 mm
D up to 230 mm
A
B
C
D
right side view offraction collector
tray platform notches
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 2-3
Installation 2Tray Setu
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Tray Inserts
A tray insert is used to hold racks in place on the fraction collector. There are two inserts available (ordered separately) depending on the type of racks used.
• Insert for two Code 200-series racks (part number 17007113)
• Insert for four Code 20-series racks (part number 17007112)
The Code 20-series tray insert is used for Code 20-series, Code 33X/34X-series, or Code 30-series racks
The Code 90-series racks do not use a separate tray insert, they sit directly on the tray platform.
To install a tray insert or Code 90-series rack, insert the drain fitting on the right side of the tray insert into the hole on the right support of the fraction collector.
Note: When using multiple fraction collectors, the same tray insert (or Code 90-series rack) must be used on all fraction collectors.
Installation 2
2-4 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Tray
Set
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The fraction collector can be configured with a variety of rack types and sizes. Refer to Appendix B, Racks for information on the available racks.
Code 20-series, Code 33X/34X-series, or Code 30-series racks
The Code 20-series, Code 33X/34X-series or Code 30-series racks are placed perpendicular to the back of the fraction collector.
1 Orient the rack so that the code number is facing forward.
2 Locate the middle slot on the back of the rack. Slide this over the pin on the tray insert.
3 Fit the middle slot on the front of the rack over the pin in the front of the tray insert.
Note: If Code 30-series racks will be used with the thermostating cuvette, a spacer for the thermal rack (part number 17007115, ordered separately) is required to raise the height of the rack and thermostating cuvette.
Code 20-series tray insert
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 2-5
Installation 2Tray Setu
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Code 200-series racks
The Code 200-series racks are placed parallel to the back of the fraction collector.
1 Orient the rack so that the code number is facing the right side.
2 Fit the rack on the tray insert so that the slots and holes on the underside of the rack align with the pins on the tray insert.
Code 200-series tray insert
Code 90-series racks
The Code 90-series racks sit directly on the tray platform. To install a Code 90-series rack, insert the drain fitting on the right side of the tray insert into the hole on the right support of the fraction collector.
Code 91 rack
Installation 2
2-6 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
3-W
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The fraction collector is shipped with the valve already installed. Ensure that the adapter end of the valve cable is connected to the fraction collector.
Dispense Tube and Fittings
The fraction collector is shipped with the dispense tube for 1/8" OD tubing installed. If you will be using the 1/16" OD plumbing package (part number 1747996) it is recommended to use the dispense tube for 1/16" OD tubing, included in the accessory package.
To switch dispense tube and/or dispense tube fittings:
1 Slide the 3-way valve assembly to the front of the X/Y arm.
2 Locate the headless nut extender tool (part number 49041032) included in the accessory package.
3 Place this tool over the dispense tube and fittings and turn clockwise to loosen.
4 Remove the old dispense tube and fittings and replace with the new ones. Use the headless nut extender tool and tighten until snug.
dispense
fittings
tube
IN
DIVERT
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 2-7
Installation 2System
Setup
System Setup
Refer to the following diagram for information on setting up multiple fraction collectors using the PREPFCTM Fraction Collector System Organizer (ordered separately, part number 210526). For instructions on assembling the system organizer refer to Appendix C, System Organizer.
If you will be using more than one fraction collector you will need the PREPFC Contact Interface Module. For information on making these connections refer to PREPFC Contact Interface Module on page 2-21.
FC 1FC 2
FC 3 FC 4
Installation 2
2-8 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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The tables and diagrams in the following sections provide detailed information on making plumbing connections.
• For information on plumbing one fraction collector refer to page 2-9.
• For information on plumbing multiple fraction collectors refer to page 2-11.
Before making the connections, locate one of the following plumbing packages (ordered separately). You will need one plumbing package for each fraction collector.
Part number Qty. Description
The PREPFC Plumbing Package 1/8" OD (part number 1747995). This package includes:
49010106 2 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing
49010107 2 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing
49010108 4 Ferrule, Tefzel, 1/4–28 for 1/8" OD tubing
541180372 3 ft Spiral wrap, 3/8" diameter, black
49040681 1 Upchurch adapter (P-681), 5/16–24 female to 1/4–28 male, Kel-F (PTCFE)
470512503 15 ft PTFE tubing, 1/8" ID x 3/16" OD
49041038 1 Upchurch flangeless nut (P-138), 3/16"/4 mm, 5/16–24, Delrin (Acetal)
49041039 1 Upchurch flangeless ferrule (P-133), 3/16", Tefzel (ETFE), blue
490032 15 ft PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot
54083751 3 Retainer, 0.375" diameter, adhesive
54096037 1 Cable clip, 0.375" diameter
470350006 10 ft Tygon drain tubing, 3/8 x 1/2
Or, the PREPFC Plumbing Package 1/16" OD (part number 1747996). This package includes:
49010109 2 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing
49010111 2 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing
49010112 4 Ferrule, Tefzel, 1/4–28 for 1/16" OD tubing
541180372 3 ft Spiral wrap, 3/8" diameter, black
490032 15 ft PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot
49010106 1 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing
49010108 1 Ferrule, Tefzel, 1/4–28 for 1/8" OD tubing
470504001 15 ft PTFE tubing, 0.04" ID x 1/16" OD, natural
54083751 3 Retainer, 0.375" diameter, adhesive
54096037 1 Cable clip, 0.375" diameter
470350006 10 ft Tygon drain tubing, 3/8 x 1/2
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 2-9
Installation 2P
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Plumbing the Fraction Collector
Using the PREPFC Plumbing Package 1/8"
Refer to the following diagram and table for instructions on making the plumbing connections for one fraction collector using the PREPFC Plumbing Package 1/8" (part number 1747995).
Use this package for flow rates between 30 and 200 mL/min.
Plumbing Connections—1/8"
Valve Connection
Tubing Fitting(s)
INLET
COMM (Common) port from detector
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.
On the other end of the tubing use the appropriate fitting to connect to the outlet of the detector.
DIVERT position
NO (Normally Open) port to waste
PTFE tubing, 1/8" ID x 3/16" OD (part number 470512503)
On one end of the tubing, use an Upchurch P-138 flangeless nut (3/16", 5/16-24, part number 49041038) and an Upchurch P-133 flangeless ferrule (3/16", part number 49041039). Connect this end to an Upchurch P-681 adapter, 5/16–24 female to 1/4–28 male (part number 49040681) then connect this to the DIVERT port.
COLLECT position
NC (Normally Closed) port
Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)
Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).
Installation 2
2-10 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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Refer to the following diagram and table for instructions on making the plumbing connections for one fraction collector using the PREPFC Plumbing Package 1/16" (part number 1747996).
Use this package for flow rates up to 30 mL/min.
Plumbing Connections—1/16"
Valve Connection
Tubing Fitting(s)
INLET
COMM (Common) port to detector
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a green Omni-Lok nut (1/16", 1/4–28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.
On the other end of the tubing use the appropriate fitting to connect to the outlet of the detector.
DIVERT position
NO (Normally Open) port to waste
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 490410106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.
COLLECT position
NC (Normally Closed) port
Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)
Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)
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Plumbing Multiple Fraction Collectors
Using the PREPFC Plumbing Package 1/8"
The following diagram shows the connections if four fraction collectors are connected using the PREPFC Plumbing Package 1/8" (part number 1747995). Refer to the table on page 2-12 for more detailed instructions on making these connections. The maximum flow rate when plumbing multiple fraction collectors with this package is 170 mL/min.
Installation 2
2-12 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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Plumbing Connections—1/8"
Valve Connection
Tubing Fitting(s)
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INLET
COMM (Common) port to detector
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.
On the other end of the tubing use the appropriate fitting to connect to the outlet of the detector.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 2
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port on FC 2.
COLLECT position
NC (Normally Closed) port
Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)
Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).
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INLET
COMM (Common) port to NO (Normally Open) port on FC 1
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port on FC 1.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 3
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port on FC 3.
COLLECT position
NC (Normally Closed) port
Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)
Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).
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INLET
COMM (Common) port to NO (Normally Open) port on FC 2
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port on FC 2.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 4
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port on FC 4.
COLLECT position
NC (Normally Closed) port
Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)
Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).
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INLET
COMM (Common) port to NO (Normally Open) port on FC 3
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 49010106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port on FC 3.
On the other end of the tubing, use a green Omni-Lok nut (1/8", 1/4–28, part number 49010107) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the IN port.
DIVERT position
NO (Normally Open) port to waste
PTFE tubing, 1/8" ID x 3/16" OD (part number 470512503)
On one end of the tubing, use an Upchurch P-138 flangeless nut (part number 49041038) and an Upchurch P-133 flangeless ferrule (part number 49041039). Connect this end to an Upchurch P-681 adapter, 5/16–24 female to 1/4–28 male (part number 49040681) then connect this to the DIVERT port.
COLLECT position
NC (Normally Closed) port
Factory-installed. Stainless steel dispense tube for 1/8" OD tubing, 2.1" long (part number 17037116)
Factory-installed. Upchurch P-386 flangeless, headless nut (1/8", 1/4–28, part number 49041054) and Upchurch P-300 flangeless ferrule (1/8", part number 49041015).
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Using the PREPFC Plumbing Package 1/16"
The following diagram shows the connections if four fraction collectors are connected using the PREPFC Plumbing Package 1/16" (part number 1747996). Refer to the table on page 2-14 for more detailed instructions on making these connections. The maximum flow rate when plumbing multiple fraction collectors with this package is 25 mL/min.
Installation 2
2-14 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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Plumbing Connections—1/16"
Valve Connection
Tubing Fitting(s)
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INLET
COMM (Common) port to detector
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a green Omni-Lok nut (1/16", 1/4–28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.
On the other end of the tubing use the appropriate fitting to connect to the outlet of the detector.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 2
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port on FC 2.
COLLECT position
NC (Normally Closed) port
Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)
Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)
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INLET
COMM (Common) port to NO (Normally Open) port on FC 1
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port on FC 1.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 3
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010108) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port on FC 3.
COLLECT position
NC (Normally Closed) port
Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)
Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)
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INLET
COMM (Common) port to NO (Normally Open) port on FC 2
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port on FC 2.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.
DIVERT position
NO (Normally Open) port to COMM (Common) port on FC 4
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port on FC 4.
COLLECT position
NC (Normally Closed) port
Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)
Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)
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INLET
COMM (Common) port to NO (Normally Open) port on FC 3
PTFE tubing, 0.04" ID x 1/16" OD (part number 470504001)
On one end of the tubing, use a blue Omni-Lok nut (1/16", 1/4–28, part number 49010109) and Tefzel ferrule (part number 49010112) to connect to the DIVERT port on FC 3.
On the other end of the tubing, use a green Omni-Lok nut (1/16", 1/4-28, part number 49010111) and Tefzel ferrule (1/16", 1/4–28, part number 49010112) to connect to the IN port.
DIVERT position
NO (Normally Open) port to waste
PTFE tubing, 1.5 mm ID x 1/8" OD (part number 490032)
On one end of the tubing, use a blue Omni-Lok nut (1/8", 1/4–28, part number 490410106) and Tefzel ferrule (1/8", 1/4–28, part number 49010108) to connect to the DIVERT port.
COLLECT position
NC (Normally Closed) port
Stainless steel dispense tube for 1/16" OD tubing, 2.1" long (part number 17037117)
Upchurch P-286 flangeless, headless nut 1/16", 1/4–28, part number 49041011) and Upchurch P-200R flangeless ferrule (1/16", part number 49041021)
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Cable Clip Installation
To keep the tubing from sliding around with arm movement you may want to use the cable clip and guides (adhesive retainers) that were included in the plumbing package. A sample configuration with four fraction collectors on the PREPFC Fraction Collector System Organizer (ordered separately, part number 210526) is shown below.
rear view—System Organizer with four PREPFC Fraction Collectors
Drain Connections
To make the drain connections:
1 Connect the barbed adapter (part number 49081701, included in the accessory package) to the outlet on the right support of the fraction collector.
2 Connect one end of the drain tubing (part number 470350006, included in the plumbing package) to the barbed adapter and place the other end into a drain or container.
Note: Position the drain/drain tubing to allow for continuous flow.
to
from
FC 1 FC 2
FC 3FC 4
detector
waste
Installation 2
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Rear Panel
Please read this entire section and refer to the rear panel diagram below before making any electrical connections. Do not turn on power to the fraction collector until all connections have been made.
1 Ethernet
2 Input/Output (I/O) ports
3 Power receptacle
4 Power switch
5 Fuse drawer
1
2
3 4 5
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Ethernet
To make the Ethernet connection to the instrument, you will need a router and Cat 5e ethernet cables.
Note: The router is not available from Gilson, Inc.
1 Connect the router to your company's network (optional)
Connect one end of a Cat 5e ethernet cable to the company network and the other end of the cable to the WAN port on the back of the router. Please consult with your System Administrator or IT Professional.
2 Connect the power supply to the router
Connect the power supply to the router. At this time, do not connect to the power source.
3 Connect the instrument to the router
Warning! Before connecting, ensure that the instrument is powered OFF.
Locate the Cat 5e ethernet cable that was included in the accessory package. Plug one end of the cable into the Ethernet port on the instrument and the other end to one of the numbered ports on the back of the router.
Installation 2
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s Input/Output Ports
You can take advantage of the powerful built-in features of your new fraction collector when you connect other equipment to the multi-purpose Input/Output (I/O) port.
The port has 14 contacts, numbered 1–14. All of the connections are paired contact closures (no voltage) except for the ANALOG IN input. The voltage range for the ANALOG IN input is from –1000mV to +1000mV.
TTL signals can be used as long as the voltage is no greater than 5V.
Some uses for the I/O contact pairs are listed below.
• When you connect a remote switch contact to the START (1–2 pair) or the STOP input (3–4 pair), other equipment can signal the fraction collector to start or stop a run.
• When you connect a remote switch contact to the ADVANCE input (5–6 pair), other equipment can signal the fraction collector to advance during a run.
• When you connect a remote switch contact to the DIVERT input (7–8 pair), other equipment can signal the fraction collector to divert (or collect) during a run.
• When you connect other equipment to the PROG 1 (9–10 pair) or the PROG 2 (11–12 pair) programmable outputs, the fraction collector can give instructions to that device based on options (e.g., Event Mark) available in PREPFC Control Software.
• When you connect a detector to the ANALOG IN input (13–14 pair), the fraction collector can collect peak-containing fractions. Refer to Making connections to a detector on page 2-20 for more information.
The I/O connection diagram shows the location of the multipurpose I/O contacts. Make the I/O connections to the terminal block connector that attaches directly to the fraction collector.
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Making connections
To make connections, you’ll need the following:
• terminal block connector
• 2-conductor cable (22–30 gauge for each wire)
• wire insulation stripper
• small-blade screwdriver
You can purchase a 6-foot piece of suitable cable (part number 709910206) or a package of five cables with identification markers (part number 36078155) from Gilson.
To make connections with the 2-conductor cable:
1 Cut the cable into pieces of appropriate length.
2 Strip about 3 mm of insulation from each end of the cable.
3 Remove the terminal block connector from the fraction collector. Insert each wire into the appropriate slot on the terminal block connector.
Note: When making connections, be sure to maintain the correct orientation of the connector relative to the port.
Push the wire all the way in; then tighten its corresponding pin screw.
4 Reconnect the terminal block connector to the fraction collector. The wires will be facing down and the pin screws facing away from the fraction collector. Push the connector in as far as it will go. It is designed to fit snugly into its receptacle.
5 Connect the opposite ends of the wires to the other equipment. Be sure to match ground connections.
Remote inputs - When making the connections, the source must provide a signal between +5V and ground or a correctly polarized TTL (0,5V) compatible signal (0 activates the input).
Programmable outputs - To use the programmable outputs, connect the leads from PROG 1 (9–10 pair) or PROG 2 (11–12 pair) to a contact-activated input of another device. Connect either lead to either input pin on the peripheral device.
ANALOG IN input - If you are connecting the ANALOG IN input to a detector, refer to Making connections to a detector on page 2-20 for more information.
6 Label each cable to identify the purpose of the connection.
terminal blockconnector
2-conductorcable
to peripheralequipment
Installation 2
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Detectors generally have one of two types of output connectors. Using the accompanying figures, determine which output connectors are used on your detector.
One type of detector has screwable binding posts built into the rear panel of the detector. To attach the fraction collector to these outputs, connect bare wire lead 14 to the “+” post on the detector and lead 13 to the GND (ground) post. Tighten the posts securely.
The other type of connector, like the one on most Gilson detectors, has fixed binding receptacles. To attach this type, you’ll need a Pomona cable adapter (part number 6374022611).
Make the connections to your fraction collector as shown below. Be sure to connect lead 14 to “+” and lead 13 to GND.
(+) (–)
to fraction collector’sANALOG IN input
(13-14 pair)
detector
Pomona cable adapter
(–)
(+)
black
red
to fraction collector’s
to recorder
to fraction collector’s
detector
to fraction collector’sANALOG IN input
(13-14 pair)
ANALOG INinput 14 (+)
ANALOG INinput 13 (–)
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PREPFC Contact Interface Module
To connect multiple fraction collectors you will need a PREPFC Contact Interface Module (part number 17017103, ordered separately). For each fraction collector you will need a Contact Interface Module cable (part number 17027114, ordered separately). Up to four fraction collectors can be connected using the PREPFC Contact Interface Module.
1 TO FC
2 Input/output ports
1 1
11
2
Installation 2
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1 Attach the end of the cable with the terminal block connector to the input/output ports on the fraction collector. The cable should be coming out on the right side of the connector.
2 Attach the other end of the cable to one of the TO FC ports on the contact interface module. Tighten the retaining screws using a flat-blade screwdriver.
3 Repeat steps 1 and 2 for each fraction collector.
Input/Output ports
The input/output ports on the contact interface module have the same functions as the input/output ports on each fraction collector. For more information refer to Input/Output Ports on page 2-18.
PREPFC Contact Interface Module (top view)
FC 4 rear panel
FC 1 rear panel
FC 3 rear panel
FC 2 rear panel
input/output ports
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Fuses
You received the fraction collector without any fuses installed. To install the fuses:
1 Locate the accessory package containing the fuse drawer appropriate for your line voltage. Discard the other fuse drawer.
2 Locate the 0.50A “T” Slo-Blo fuses (5 x 20 mm size), also included in the accessory package.
3 Install the fuse(s) into the fuse drawer. The fuse drawer for 100–120V accepts one fuse. The fuse drawer for 220–240V accepts two fuses.
4 Insert the fuse drawer into its receptacle in the fraction collector. See rear panel diagram on page 2-16.
Power Connection
Locate the appropriate power cord for your line voltage. Discard the other power cord.
Use the power cord to connect the fraction collector to an AC power source.
fuse installation for 100–120V fuse installation for 220–240V
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 3-1
Operation 3
The PREPFCTM Fraction Collector is controlled by PREPFC Control Software which is run on a handheld Pocket PC running Microsoft® Windows Mobile®. The Pocket PC is the dedicated fraction collector controller.
If you have not already done so, install the software. Refer to the PREPFC Control Software Installation Overview and Instructions (part number LT350021) supplied with the PREPFC Control Software and also found on the PREPFC Control Software Documentation CD (part number LT2106270CD).
Operation 3
3-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
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The following steps must be completed in order to operate the fraction collector:
1 Connect the router to a power source and wait 30 seconds.
2 Power ON the fraction collector and wait 1 minute.
If the status light on the front of the fraction collector begins blinking, there is a problem with the fraction collector. See page 5-4.
3 Connect the Pocket PC to the router (not required with a wireless router).*
4 Launch PREPFC Control Software on the Pocket PC.
Run Setup
The PREPFC must be set up using PREPFC Control Software. Refer to the PREPFC Control Software User’s Guide included on the PREPFC Control Software Documentation CD (part number LT2106270CD) for this information.
The remote inputs and programmable outputs are available when the fraction collector is used with other equipment.
• Using the programmable outputs, the fraction collector sends instructions to other equipment. Refer to Programmable Outputs on page 3-3 for more information.
• Using the remote inputs, the fraction collector receives instructions from other equipment. Refer to Remote Inputs on page 3-5 for more information.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 3-3
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Programmable Outputs
The fraction collector has several jobs to accomplish during normal operation. It may be waiting, diverting/draining, or collecting. The PROG 1 (9–10 pair) or the PROG 2 (11–12 pair) programmable outputs can be used to direct other equipment (a chart recorder or pump, for example) to be active only when the fraction collector is doing one of those jobs. The programmable outputs are controlled through PREPFC Control Software.
The following diagram illustrates each of the options available for the programmable outputs and assumes the initial contact state is opened (OFF). Refer to the text after the diagram for an explanation of each of these options.
A On After Initial Wait - The state of the contact is closed (ON) at the beginning of the first window and stays closed (ON) until the end of the run or until the run is stopped.
B On During Initial Wait - The state of the contact is closed (ON) at the beginning of the run and then is opened (OFF) when the first collection window starts.
Operation 3
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D Pulse at End of Run - Pulses the contact at the end of the run.
E Pulse at Peak Start - Pulses the contact at the start of each peak. The contact is only pulsed when the peak is detected inside a collection window (the other equipment will be idle when outside a collection window). This option applies only to collection by Level or Slope.
F On During Peak - The state of the contact is closed (ON) during the peak and then is opened (OFF) at the end of the peak. The contact is only closed (ON) when the peak is detected inside a collection window (the other equipment will be idle when outside a collection window). This option applies only to collection by Level or Slope.
G Pulse at Valve Switch (Event Mark) - Pulses the contact every time the valve is switched from DIVERT to COLLECT or from COLLECT to DIVERT. The contact is only pulsed when inside a collection window (the contact will be idle when outside a collection window).
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 3-5
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Remote Inputs
The START, STOP, ADVANCE, and DIVERT inputs can perform the functions listed below.
Note: The run screen in PREPFC Control Software must be open in order for the PREPFC to be controlled using the remote inputs.
START (1–2 pair)
• Remote start of the run.
STOP (3–4 pair)
• Remote stop of the run. The fraction collector will not home.
ADVANCE (5–6 pair)
• Advances to the next tube only during the run.
If the valve is in the DIVERT position, it will remain in the DIVERT position.
If valve is in the COLLECT position, the valve will switch to DIVERT, move to the next tube, and then switch back to COLLECT.
DIVERT (7–8 pair)
• Changing the contact state toggles the valve between the DIVERT and COLLECT positions.
• Switching the valve to DIVERT will prevent any sample from being collected.
• Will advance to the next tube if the current tube has sample.
• Switching the valve to COLLECT will allow sample to be collected, when appropriate.
• Valve will not start collecting when in peak mode unless in a peak.
• Valve will start collecting when in a non-peak mode.
Collection Modes
PREPFC Control Software allows collection by Slope, Level, Time, and Volume. Refer to the PREPFC Control Software User’s Guide included on the PREPFC Control Software Documentation CD (part number LT2106270CD) for information on setting collection mode parameters.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 4-1
Maintenance 4
To obtain optimum performance and maximum life from the PREPFCTM Fraction Collector, it is important to keep the instrument well-maintained.
This section contains the following information to help you maintain your fraction collector.
• Cleaning
• Transporting the Fraction Collector
• Replacing Dispense Tube and Fittings
• Replacing the 3-Way Valve
• Replacing a Fuse
Maintenance 4
4-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Cle
anin
g Cleaning
The fraction collector should be cleaned occasionally using a dry, clean cloth. Or, if necessary, use a cloth dipped in soapy water. If liquid is accidentally spilled on the fraction collector, wipe the instrument using a dry, clean cloth.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 4-3
Maintenance 4Tran
spo
rting
the Fractio
nC
ollecto
r
Transporting the Fraction Collector
When moving the fraction collector to another location, do not use the arm as a handle. Doing so may damage the unit or cause misalignment of the X/Y positioning. You may carry the fraction collector using the large rectangular opening in the front of the unit.
Maintenance 4
4-4 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rep
laci
ng
Dis
pen
se T
ub
e an
d F
itti
ng
s Replacing Dispense Tube and Fittings
To replace dispense tube and/or dispense tube fittings:
1 Slide the 3-way valve assembly to the front of the X/Y arm.
2 Locate the headless nut extender tool (part number 49041032) included in the accessory package.
3 Place this tool over the dispense tube and fittings and turn clockwise to loosen.
4 Remove the old dispense tube and fittings and replace with the new ones. Use the headless nut extender tool and tighten until snug.
dispense
fittings
tube
IN
DIVERT
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 4-5
Maintenance 4R
eplacin
g th
e 3-Way V
alve
Replacing the 3-Way Valve
To replace the 3-way valve, follow the procedures in this section.
Before you Begin
1 Switch power OFF to the fraction collector.
2 Slide the 3-way valve assembly to the front of the X/Y arm.
3 Unplug the valve cable from the fraction collector.
4 Remove all fittings and tubing (including the dispense tube) from the valve. For more information on removing the dispense tube refer to Replacing Dispense Tube and Fittings on page 4-4.
Remove the Valve
1 Find the location where the IGUS cable is attached to the track.
2 Grip the IGUS cable on the last link (next to the mounting bracket) and rotate to detach.
3 Remove the valve cable from the IGUS cable and from the guide on the valve bracket.
4 Remove the two screws holding the valve on the valve bracket.
track
overhead view—IGUS cable on track
back of fraction collector
mounting
last link
bracket
of IGUScable
valvecable
screws
valve bracket
Maintenance 4
4-6 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rep
laci
ng
the
3-W
ay V
alve Replace the Valve
1 Reattach the valve to the valve bracket making sure that the DIVERT port is located at the top.
2 Place the valve cable in the guide on the valve bracket. Route the wiring behind the dispense tube (between the dispense tube and the valve bracket).
3 Place the valve cable in the IGUS cable.
4 Place the adapter end of the valve cable on top of the mounting bracket on the IGUS cable.
5 Reassemble the IGUS cable.
6 Plug the adapter end of the valve cable into the fraction collector.
7 Reattach all tubing and fittings (including the dispense tube).
Adjust the Valve
The position of the valve may need to be adjusted slightly after all the tubing and fittings have been replaced.
If the dispense tube is not centered in the opening on the track do the following:
1 Slightly loosen each of the screws on the back of the valve bracket.
2 Adjust the valve so the dispense tube is centered in the opening of the track.
3 While holding the valve in position, tighten the screws on the back of the valve bracket.
DIVERT
IN
dispense tube
valve cable
guide
to IGUS cable
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 4-7
Maintenance 4R
eplacin
g a Fu
se
Replacing a Fuse
A blown fuse may indicate the existence of another problem in the instrument. If the replacement fuses blow, don’t try others. Contact your local distributor. See Before Calling Us on page 5-5.
To change a fuse, follow these steps.
1 Disconnect the power cord from the power outlet and from the rear panel receptacle.
2 Locate the fuse drawer on the rear panel. See page 2-16 if necessary.
3 Insert a small screwdriver into the notch located to the left of the fuse drawer.
4 Twist the screwdriver to open and remove the fuse drawer.
• The fuse drawer for a 100–120 voltage selection contains one 0.50A “T” Slo-Blo fuse (5 x 20 mm size).
• The fuse drawer for a 220–240 voltage selection contains two 0.50A “T” Slo-Blo fuses (5 x 20 mm size).
5 Remove the old fuse(s) and insert the new fuse(s)
6 Insert the fuse drawer into its receptacle in the fraction collector.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 5-1
Troubleshooting 5
If you encounter a problem while operating the fraction collector, refer to the following pages. If you cannot solve or isolate the problem, contact your Gilson-authorized representative. See Before Calling Us on page 5-5.
This chapter provides information on the following topics:
• Error Messages
• Troubleshooting
• Repair and Return Policies
Troubleshooting 5
5-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Erro
r M
essa
ges Error Messages
Error # Error Text Description
0 Normal
10 Bad command An illegal command was received.
11 Range error An out-of-range parameter was received.
30 X Homing The unit was not able to home itself.
31 Y Homing The unit was not able to home itself.
32 X Unhomed A motion was requested when the unit was not homed state.
33 Y Unhomed A motion was requested when the unit was not homed state.
40 Tube Queue error Queue already full.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 5-3
Troubleshooting 5Tro
ub
lesho
otin
g
Troubleshooting
Ethernet connections
For communication to occur, the PREPFCTM Fraction Collector must be connected via an Ethernet connection to a router. If there are communication problems between the fraction collector and the Pocket PC, verify that the procedure below has been followed.
1 Connect the router to a power source and then wait 30 seconds for the router to initialize.
2 With the fraction collector powered OFF, connect one end of an Ethernet cable to a numbered port on the back of the router and the other end to the Ethernet port on the rear panel of the fraction collector.
3 Power the fraction collector ON and then wait 1 minute for it to initialize. The fraction collector will be assigned an IP address via DHCP from the router at this time.
4 This step is not necessary with a wireless router.Using a Cat 5e ethernet cable, connect one end of the cable to the connector on the Ethernet CF (Compact Flash) Card for Pocket PC and the other end to a numbered port on the back of the router.
Use the Gilson Ethernet Utility (part number 21067530, included in the accessory package) to further check communication or contact your Gilson-authorized representative.
Troubleshooting 5
5-4 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Tro
ub
lesh
oo
tin
g Fraction fluid is missing the vessel
• Check to be sure the template and the rack code in PREPFCTM Control Software matches the racks you are using.
• Check location of racks and tray insert; the racks may be out of position on the tray insert or the tray insert may not be seated properly in the tray platform.
• Check mechanical X/Y arm assembly for alignment. Turn the unit off and then restart it. If it is still out of alignment, contact your Gilson-authorized representative.
3-way valve not functioning or functioning incorrectly
• Check connection to the fraction collector. Plugging or unplugging valve with the power on is not suggested as this may cause the machine to reset.
• Check the fluid connections to the valve for proper orientation.
• Contact your Gilson-authorized representative; the valve may be defective.
Unit not operational
• Make sure the power is turned on.
• Check AC power cord connections
• Try a different AC outlet.
• Check fuse(s); replace if necessary.
• Contact your Gilson-authorized representative.
Status light blinking
• Check for obstructions.
• Home the fraction collector.
• Switch the power to the fraction collector OFF then ON.
• Restart controlling program.
• Contact your Gilson-authorized representative.
Unit blows fuses
• Contact your Gilson-authorized representative.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide 5-5
Troubleshooting 5R
epair an
d R
eturn
Policies
Repair and Return Policies
Before Calling Us
Gilson-authorized representatives will be able to serve you more efficiently if you have the following information:
• the serial number and model number of the instruments involved. The serial number is located on the inside of the fraction collector, on the right support. Refer to the diagram.
• the installation procedure you used
• list of concise symptoms
• list of operating procedures and conditions you were using when the problem arose
• list of other devices connected to the fraction collector and a description of those connections
• list of other electrical connections in the room
Warranty Repair
Units covered under warranty will be repaired and returned to you at no charge. If you have any questions about applicability, please contact your local distributor.
Non-Warranty Repair
For out-of-warranty repairs, contact your local distributor. A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment, if necessary.
Rebuilt Exchange
For some units, rebuilt exchange components are available. Contact your local distributor for details.
serial number
Troubleshooting 5
5-6 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rep
air
and
Ret
urn
Po
licie
s Return Procedure
Contact your local distributor’s Customer Service Department to obtain authorization before returning any Gilson equipment. To return a piece of equipment:
• Carefully pack the unit to prevent damage in transit. Check with your distributor regarding proper method of shipment. No responsibility is assumed by Gilson or your distributor for damage caused by improperly packaged instruments. Indicate the authorization on the carton and on the packing slip.
• Always insure for the replacement value of the unit.
• Include a description of symptoms, your name, address, phone number, and purchase order to cover repair costs, return and shipping charges, if your institution requires it.
Unit End-of-Life
When a unit reaches the end of its useful life, refer to www.gilson.com for directions and information on the end-of-life policy. This is in accordance with the European Union Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE).
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide A-1
Replacement Parts and Accessories A
Rack Accessories
Part Number Description
17007111 Tray platform
17007112 Tray insert for four Code 20-series racks
17007113 Tray insert for two Code 200-series racks
Valve
Part Number Description
17037251 3-way valve, PREPFC
Tubing and Fittings
Part Number Description
49010106 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing
49010107 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/8" OD tubing
49010108 Ferrule, Tefzel, 1/4–28 for 1/8" OD tubing
49010109 Blue Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing
49010111 Green Omni-Lok™ nut, polypropylene, 1/4–28 for 1/16" OD tubing
49010112 Ferrule, Tefzel, 1/4–28 for 1/16" OD tubing
49041038 Upchurch flangeless nut (P-138), 3/16"/4 mm, 5/16-24, Delrin (Acetal), natural
49041039 Upchurch flangeless ferrule (P-133), 3/16", Tefzel (ETFE), blue
Appendix A
A-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rep
lace
men
t Par
ts a
nd
Acc
esso
ries
49040681 Upchurch adapter (P-681), 5/16–24 female to 1/4–28 male, 0.125 in thru hole, Kel-F (PTCFE)
49041054 Upchurch P-386 flangeless, headless nut, 1/8", 1/4-28
49041015 Upchurch P-300 flangeless ferrule, 1/8"
49041011 Upchurch P-286 flangeless, headless nut 1/16", 1/4–28
49041021 Upchurch P-200R flangeless ferrule, 1/16"
470504001 PTFE tubing, 0.04" ID x 1/16" OD, natural
470512503 PTFE tubing, 1/8" ID x 3/16" OD
490032 PTFE tubing, 1.5 mm ID x 1/8" OD, clear, per foot
541180372 Spiral wrap, 3/8" diameter, black
54083751 Retainer, 0.375" diameter, adhesive
54096037 Cable clip, 0.375" diameter
17037116 Stainless steel dispense tube, 2.1" x 0.120", for 1/8" OD tubing
17037117 Stainless steel dispense tube, 2.1" x 0.063", for 1/16" OD tubing
470350006 Tygon drain tubing, 3/8 x 1/2
49081701 Elbow, 1/8 NPT to 3/8 barb
49041032 Upchurch P-297 headless nut extender tool
Tubing and Fittings (Continued)
Part Number Description
Cables and I/O Accessories
Part Number Description
6730054007 0.5 amp T-0.5 replacement fuse, Slo-Blo
6770100411 Fuse drawer, 220V
6770100412 Fuse drawer, 110V
36078142 Cat 5e ethernet cable
7080316106 Power cord, detachable, 220V
7080318107 Power cord, 18/3, 110V
638314512 Terminal block connector, 14-pin
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide A-3
Appendix AR
eplacem
ent Parts an
d A
ccessories
Miscellaneous
Part Number Description
17017103 PREPFC Contact Interface Module to connect up to four fraction collectors
17027114 Cable to connect from PREPFC Contact Interface Module to each fraction collector (one cable required for each fraction collector)
210526 System Organizer, PREPFC
21052609 Pocket PC Guard for HP iPAQ hx2100- or hx2400-Series
2106270Rxx PREPFCTM Control Software
21067530 Gilson Ethernet Utility
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-1
Racks B
The fraction collector can be configured with a variety of rack types and sizes. The following pages describe the racks that can be purchased for use on the fraction collector. Refer to Chapter 2, Installation for rack installation procedures.
Appendix B
B-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 20 rackFor 108 tubes
Material: polypropylene
Vessels and maximum capacity: 10 x 100 mm (4.5 mL)
Part number: 150425
Requires tray insert (part number 17007112)
Code 21 rackFor 60 tubes
Material: polypropylene
Vessels and maximum capacity: 13 x 100 mm (9 mL)
Part number: 150422
Requires tray insert (part number 17007112)
Code 22 rackFor 44 tubes
Material: polypropylene
Vessels and maximum capacity: 18 x 100 mm (25 mL)
18 x 150 mm (32 mL)
Part number: 150424
Requires tray insert (part number 17007112)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-3
Appendix BR
acks
Code 22U rackFor 44 tubes
Material: polypropylene
Vessels and maximum capacity: 10–18 mm x 100–180 mm
Part number: 150498
Requires tray insert (part number 17007112)
Code 23 rackFor 44 scintillation vials
Material: polypropylene
Vessels and maximum capacity: 17 x 55 mm (6.8 mL)
17 x 65 mm (8 mL)
Part number: 150426
Requires tray insert (part number 17007112)
Code 23W rackFor 44 Waters WISP vials
Material: polypropylene
Vessels and maximum capacity: 15 x 45 mm (4 mL)
Part number: 270433
Requires tray insert (part number 17007112)
Appendix B
B-4 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 24 rackFor 14 scintillation vials
Material: polypropylene
Vessels and maximum capacity: 28 x 57 mm (20 mL)
28 x 60 mm (20 mL)
Part number: 150427
Requires tray insert (part number 17007112)
Code 28 rackFor 108 tubes
Material: polypropylene
Vessels and maximum capacity: 10 x 65 mm (3 mL)
10 x 75 mm (3.5 mL)
Part number: 150420
Requires tray insert (part number 17007112)
Code 29 rackFor 60 tubes
Material: polypropylene
Vessels and maximum capacity: 12 x 75 mm (3.5 mL)
13 x 75 mm (5 mL)
Part number: 150429
Requires tray insert (part number 17007112)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-5
Appendix BR
acks
Code 30 rackFor 60 vials
Material: aluminum
Vessels and maximum capacity: 12 x 32 mm (2 mL)
Part number: 2704430
Shown installed in thermostating cuvette
Requires tray insert (part number 17007112)
Code 31 rackFor 108 vials
Material: aluminum
Vessels and maximum capacity: 7 x 40 mm (0.7 mL)
Part number: 2704431
Requires tray insert (part number 17007112)
Code 32 rackFor 60 tubes
Material: aluminum
Vessels and maximum capacity: 13 x 65 mm (6 mL)
13 x 100 mm (9 mL)
Part number: 2704432
Shown installed in thermostating cuvette
Requires tray insert (part number 17007112)
Appendix B
B-6 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 33 rackFor 14 scintillation vials
Material: aluminum
Vessels and maximum capacity: 28 x 57 mm (20 mL)
Part number: 2704433
Requires tray insert (part number 17007112)
Code 34 rackFor 36 Waters WISP vials
Material: aluminum
Vessels and maximum capacity: 15 x 45 mm (4 mL)
Part number: 2704434
Requires tray insert (part number 17007112)
Code 37 rackFor 56 vials and one scintillation vial
Material: aluminum
Vessels and maximum capacity: 56 12 x 32 mm (2 mL)
1 28 x 60 mm (20 mL)
Part number: 2704437
Requires tray insert (part number 17007112)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-7
Appendix BR
acks
Code 38 rackFor 96 vials and one scintillation vial
Material: aluminum
Vessels and maximum capacity: 96 7 x 41 mm (0.7 mL)
1 28 x 60 mm (20 mL)
Part number: 2704438
Requires tray insert (part number 17007112)
Appendix B
B-8 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 41 rackFor 108 tubes
Material: stainless steel
Vessels and maximum capacity: 10 x 75 mm (3.5 mL)
Part number: 2957850
Requires tray insert (part number 17007112)
Code 43 rackFor 80 tubes
Material: stainless steel
Vessels and maximum capacity: 13 x 100 mm (9 mL)
Part number: 2954852
Part number: Requires tray insert (part number 17007112)
Code 45 rackFor 44 tubes
Material: stainless steel
Vessels and maximum capacity: 16 x 150 mm tubes
Part number: 2954851
Part number: Requires tray insert (part number 17007112)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-9
Appendix BR
acks
Code 60 rackFor four bottles
Material: stainless steel
Vessels and maximum capacity: 180 mL
250 mL
Part number: 2954651
Requires tray insert (part number 17007112)
Code 61 rackFor four bottles
Material: stainless steel
Vessels and maximum capacity: 180 mL
250 mL
Part number: 2954715
Requires tray insert (part number 17007112)
Appendix B
B-10 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 200 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 13 x 100 mm (9 mL)
Part number: 2504600
Requires tray insert (part number 17007113)
Code 202 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 10.25 x 47 mm (2.5 mL)
Part number: 2504602
Requires tray insert (part number 17007113)
Code 203 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 10.25 x 64 mm (3 mL)
Part number: 2504603
Requires tray insert (part number 17007113)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-11
Appendix BR
acks
Code 204 rackFor 27 scintillation vials
Material: aluminum
Vessels and maximum capacity: 28 x 57 mm (20 mL)
Part number: 2504604
Requires tray insert (part number 17007113)
Code 206 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 13 x 75 mm (4 mL)
Part number: 2504606
Requires tray insert (part number 17007113)
Code 207 rackFor 75 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 16 x 100 mm (12 mL)
Part number: 2504607
Requires tray insert (part number 17007113)
Appendix B
B-12 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 208 rackFor 70 tubes
Material: aluminum
Vessels and maximum capacity: 18 x 150 mm (25 mL)
Part number: 2504608
Requires tray insert (part number 17007113)
Code 209 rackFor 96 vials
Material: aluminum
Vessels and maximum capacity: 12 x 32 mm (2 mL)
Part number: 2504609
Requires tray insert (part number 17007113)
Code 210 rackFor 75 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 16 x 75 mm (10 mL)
Part number: 2504610
Requires tray insert (part number 17007113)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-13
Appendix BR
acks
Code 211 rackFor 9 Boston round screw-cap bottles
Material: aluminum
Vessels and maximum capacity: 48 x 113 mm (125 mL)
Part number: 2504611
Requires tray insert (part number 17007113)
Code 212 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 48 13 x 100 mm (9 mL)
48 13 x 75 mm (4 mL)
Part number: 2504612
Requires tray insert (part number 17007113)
Note: Place the shorter tubes into the notched tube locations.
Appendix B
B-14 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 213 rackFor 74 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 37 16 x 100 mm (12 mL)
37 16 x 75 mm (10 mL)
Part number: 2504613
Requires tray insert (part number 17007113)
Note: Place the shorter tubes into the notched tube locations.
Code 214 rackFor 96 Vacutainer tubes
Material: aluminum
Vessels and maximum capacity: 48 10.25 x 47 mm (2.5 mL)
48 10.25 x 64 mm (3 mL)
Part number: 2504614
Requires tray insert (part number 17007113)
Note: Place the shorter tubes into the notched tube locations.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-15
Appendix BR
acks
Code 216 rackFor 60 Waters WISP vials
Material: aluminum
Vessels and maximum capacity: 15 x 45 mm (4 mL)
Part number: 2504616
Requires tray insert (part number 17007113)
Code 217 rackFor 96 tubes
Material: aluminum
Vessels and maximum capacity: 10 x 75 mm (4 mL)
Part number: 2504617
Requires tray insert (part number 17007113)
Code 219 rackFor 27 vials
Material: aluminum
Vessels and maximum capacity: 30 x 95 mm (40 mL)
Part number: 2504619
Requires tray insert (part number 17007113)
Appendix B
B-16 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 220 rackFor 70 vials
Material: aluminum
Vessels and maximum capacity: 17 x 62 mm (7 mL)
Part number: 2504620
Requires tray insert (part number 17007113)
Code 222 rackFor 27 conical bottom tubes
Material: aluminum
Vessels and maximum capacity: 29 x 115 mm (50 mL)
Part number: 2504622
Requires tray insert (part number 17007113)
Code 223 rackFor 96 tubes with screw caps
Material: aluminum
Vessels and maximum capacity: 16 x 100 mm (10 mL)
Part number: 2504623
Requires tray insert (part number 17007113)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-17
Appendix BR
acks
Code 224 rackFor 192 vials
Material: aluminum
Vessels and maximum capacity: 8.5 x 41 mm
Part number: 2504624
Requires tray insert (part number 17007113)
Code 225 rackFor 30 vials
Material: aluminum
Vessels and maximum capacity: 25 x 150 mm (45 mL)
Part number: 2504625
Requires tray insert (part number 17007113)
Code 226 rackFor 96 Waters WISP vials
Material: aluminum
Vessels and maximum capacity: 15 x 45 mm (4 mL)
Part number: 2504626
Requires tray insert (part number 17007113)
Appendix B
B-18 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 239 rackFor 40 tubes
Material: aluminum
Vessels and maximum capacity: 25 x 200 mm
Part number: 2504639
Requires tray insert (part number 17007113)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-19
Appendix BR
acks
Code 330For 60 vials
Material: aluminum
Vessels and maximum capacity: 12 x 32 mm (2 mL)
Part number: 260440031
Requires tray insert (part number 17007112)
Code 333For 14 scintillation vials
Material: aluminum
Vessels and maximum capacity: 20 mL
Part number: 260440036
Requires tray insert (part number 17007112)
Code 341For 108 tubes
Material: aluminum
Vessels and maximum capacity: 10 x 75 mm (4 mL)
Part number: 260440039
Requires tray insert (part number 17007112)
Appendix B
B-20 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Rac
ks Code 343For 80 tubes
Material: aluminum
Vessels and maximum capacity: 13 x 100 mm (9 mL)
Part number: 260440025
Requires tray insert (part number 17007112)
Code 345For 44 tubes
Material: aluminum
Vessels and maximum capacity: 16 x 150 mm (20 mL)
Part number: 260440041
Requires tray insert (part number 17007112)
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide B-21
Appendix BR
acks
Code 91 rackFor 11 Kimax or Pyrex bottles
Material: aluminum
Vessels and maximum capacity: 500 mL
Part number: 17041950
Code 92 rackFor eight Kimax or Pyrex bottles
Material: aluminum
Vessels and maximum capacity: 1000 mL
Part number: 17041951
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide C-1
System Organizer C
Use the instructions on the following pages to assemble the PREPFCTM Fraction Collector System Organizer.
Parts
The PREPFC Fraction Collector System Organizer (part number 210526, ordered separately) includes the following:
Part number Description
21052650 Right side assembly
21052651 Left side assembly
21052601 42" wide shelf, qty 2
21052303T 37" wide shelf stiffener, qty 2
21052602 Corner brace, qty 4
4013192109 10–32 x 5/8" socket cap screws, qty 20
4011141045 6–32 x 1/4" Phillips screws, qty 20
43115621 5/32" ball point Allen wrench
Appendix C
C-2 Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide
Syst
em O
rgan
izer Attaching the Shelf Stiffeners
1 Align the holes on one of the shelf stiffeners with the holes on one of the shelves.
2 Using a Phillips screwdriver and the Phillips screws, attach the shelf stiffener to the shelf.
3 Repeat the process for the second shelf and shelf stiffener.
Attaching the Shelves1 Lay the left side assembly with the beveled (slanted) edge down and the feet
facing you. The color strip faces the left. (If the color strip faces the right in this position, you have the right side assembly.)
2 In the first and sixth hole from the top, insert and slightly finger tighten a socket cap screw on the left nut strip. Then insert and slightly finger tighten a socket cap screw in the same position on the right nut strip.
3 Position the first shelf by sliding its notches between the side assembly and the socket cap screws. Tighten the socket cap screws using the wrench. The shelf will be at a 90° angle to the side assembly.
4 Attach another shelf using the procedure outlined in the previous step.
5 Turn the left side assembly and shelves so the color strip is facing up.
6 To the right of the left side assembly and attached shelves, lay the right side assembly with the beveled edge down and the feet facing you. The color strip faces the right.
7 Insert and slightly finger tighten the socket cap screws in the same locations you attached the shelves to the left side assembly.
8 Turn the right side assembly so the color strip is facing up.
9 Align the socket cap screws on the right side assembly to the shelves attached to the left side assembly. Move the right side assembly until the notches of the shelves slide between the socket cap screws and the right side assembly. Tighten the socket cap screws with the wrench.
10 Lift the system organizer and stand it in its upright position. The front of the system organizer has the two color strips.
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide C-3
Appendix CSystem
Org
anizer
Attaching the Corner Braces1 On the rear of the system organizer, locate the sliding nut strips within the
grooves of the right and left side assemblies and one of the shelves.
2 Insert socket cap screws into the three holes in one of the corner braces.
3 Align the socket cap screws and corner brace with nuts in the sliding nut strips. Slightly finger tighten the socket cap screws.
4 After the corner brace is in place, tighten all three socket cap screws with the wrench.
5 Repeat the process for the other three corner braces.
sliding nut strips
cornerbrace
Gilson PREPFCTM Fraction Collector (Ethernet) User’s Guide D-1
Pocket PC Guard D
The Pocket PC Guard for HP iPAQ hx2100- or hx2400-Series is used in conjunction with a laptop computer locking cable to secure the Pocket PC. The laptop computer locking cable must be purchased separately and is not available from Gilson, Inc.
Parts
The Pocket PC Guard for HP iPAQ hx2100/2400-Series (part number 21052609) includes the following:
• Holder
• Cover
• Screws, 4
• 5/64" Allen wrench
Assembly1 Place the Pocket PC in the Pocket PC Guard
holder.
2 Place the Pocket PC Guard cover over the holder.
3 Locate the four screws included with the Pocket PC Guard.
4 Insert the screws in the locations shown in the diagram and tighten using the Allen wrench included with the Pocket PC Guard.
5 Insert the lock end of the laptop computer locking cable into one of the slots near the bottom of the Pocket PC Guard. The cable can be inserted in either slot.
exploded view of Pocket PC Guard
cover
holder
slot
slot