Prep-n-Go™ Buffer - Applied Biosystems · PRODUCT BULLETIN HUMAN IDENTIFICATION Prep-n-Go™...
Transcript of Prep-n-Go™ Buffer - Applied Biosystems · PRODUCT BULLETIN HUMAN IDENTIFICATION Prep-n-Go™...
PRODUCT BULLETINHUMAN IDENTIFICATION
Prep-n-Go™ Buffer
What is the Prep-n-Go™ Buffer?
Prep-n-Go™ Buffer is a best-in-class solution designed to enable high quality direct PCR amplification of non-FTA® reference samples without the need for heat incubation, extraction or purification.
Prep-n-Go™ Buffer enables increased STR profile quality with fewer intermediate steps than currently available solutions for non-FTA® reference samples.
• Eliminates the requirement for heat incubation or additional workflow steps
• Designed to provide consistent, interpretable peaks above the detection threshold and high quality, well- balanced STR profiles
• Helps simplify automation requirements and expand throughput capabilities
• Helps maximize sample integrity and data quality
• Developed according to stringent performance standards to enable higher data reproducibility, reduced artifacts, and increased success rates with Applied Biosystems® STR direct amplification solutions
Why should I choose Prep-n-Go™ Buffer?
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Figure 1. An example of a profile generated from a Bode Buccal DNA Collector™ sample using the Prep-n-Go™ Buffer and the Identifiler® Direct kit.
Figure 2. An example of a profile generated from a Bode Buccal DNA Collector™ sample using the Prep-n-Go™ Buffer and the NGM SElect™ kit.
Frequently Asked Questions
Q. How does Prep-n-Go™ Buffer perform with other sample collection methods or other STR kits?A. Prep-n-Go™ Buffer has been validated internally for direct PCR amplification of reference samples collected with the Bode Buccal DNA Collector™ using the Identifiler® Direct kit. We have demonstrated initial feasibility using the Prep-n-Go™ Buffer with the NGM™ and NGM SElect™ PCR Amplification Kits and samples collected with buccal swabs. More extensive evaluations of these workflows are currently being investigated.
Q. Where can I find detailed information on the Prep-n-Go™ workflow?A. Details on the Prep-n-Go™ workflow are available in the Identifiler® Direct PCR Amplification Kit User Guide (P/N 4415125). If your laboratory is interested in using the Prep-n-Go™ Buffer with the NGM™ and NGM SElect™ kits or for direct amplification of samples collected with buccal swabs, please contact your HID support specialist.
Q. How many reactions can be performed with one kit?A. Prep-n-Go™ Buffer is available in one package size of 1,000 reactions. This packaging solution is designed to enable processing of Bode Buccal DNA Collector™ samples. An additional larger package size designed to enable processing with buccal swabs (which require a larger buffer volume) is in development.
ORDERING INFORMATION
Description Quantity P/N
Prep-n-Go™ Buffer 1,000 tests, 2 μL/test 4467079
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Buccal DNA Collector is a trademark of Bode Technology.