Přenosy jader
-
Upload
melissan-ian -
Category
Documents
-
view
20 -
download
0
description
Transcript of Přenosy jader
Přenosy jader
J. Fulka, Jr.
CBTTN a VÚŽV
Praha
Fertilization – transformation of meiotic cells into mitotic cells
Gametes – terminally differentiated and highly specialized
Genomes - (oocyte, sperm) transcriptionally inactive after fertilization
• One cell stage embryo is totipotent
• Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES
A) Sheep
B) Mouse
C) Human
D) Rabbit
E) Bovine
F) Pig
G) Monkey
Mouse embryo
Human embryo
PB
MPNFPN
FPN
MPN
5-MeC
Human embryo
PB
PN
PN MPN
FPN
49% of human 1-cell stage embryos showed abnormal demethylation pattern
2PB
FP
MP
MP
Wilmut, I et al. (1997)
Viable offspring derived from fetal and adult mammalian cells.
Nature 385, 810-813
A
B
AB
AB
AB
AB
Enukleace
Přenos jádra
What do we need??
Oct4 Cdx2
DAPI merge
Nanog
Oct4 DAPI
DifferentiationβIII tubulin
TROMA 1
DAPI
MF20
+ EB formation
Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň
Pacient ESc linie
Producing primate embryonic stem cells bysomatic cell nuclear transfer
Nature - doi:10.1038/nature06357
…. potřebujeme vůbec přenosy jader?
Děkuji za pozornost