Precautions AXIOM REAGENTS ARE FOR RESEARCH ......Module 3: Wash Buffer A P/N 901446, Wash Buffer B...
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PackageInsert For Research Use Only Not for use in diagnostic procedures. Intended Use The Affymetrix Axiom ® 2.0 Reagent Kit enables genotyping of SNPs and in/dels on Axiom ® Genome-Wide Array Plates and Axiom ® myDesign ™ Array Plates. This kit is available in a 96, 384, and 4x24 reaction format. The kits include the reagents and control DNA (human) required to: ■ ■ Produce hybridization-ready targets for Axiom Genome-Wide Array Plates and Axiom myDesign Array Plates. ■ ■ Process array plates on the Affymetrix GeneTitan ® Multi-Channel Instrument. This product is for research purposes only and is not intended for use in the diagnosis of disease. Ordering Information Reagent Kits Product Name Description P/N Axiom ® 2.0 Reagent Kit Sufficient for one 96 array plate 901758 Axiom ® 2.0 384HT Reagent Kit Sufficient for one 384HT array plate 902245 Axiom ® 2.0 Reagent Kit 4x24 Reactions Sufficient for four 24 array plates 902798 Supporting Products For a complete list of supporting products please visit www.affymetrix.com. Affymetrix ® products can be purchased directly from Affymetrix in the United States, and many European and Asian countries. For all other territories, please view a list of our distribution partners, which can be located at: www.affymetrix.com/site/contact/index.affx. Instructions for Use For instructions on using this reagent kit and Axiom Genome-Wide Array Plates and Axiom myDesign Array Plates, refer to the following documentation: ■ ■ Axiom ® 2.0 Assay Automated Workflow User Guide, P/N 702963 ■ ■ Axiom ® 2.0 Assay Manual Workflow User Guide, P/N 702990 ■ ■ Axiom ® 2.0 Assay Automated Target Prep Protocol QRC, P/N 702962 ■ ■ Axiom ® 2.0 Manual Target Prep Protocol QRC, P/N 702989 ■ ■ GeneTitan ® MC Protocol for Axiom 2.0 Array Plate Processing QRC, P/N 702988 ■ ■ Axiom ® 2.0 gDNA Sample Prep Protocol QRC, P/N 702987 ■ ■ Axiom ® 2.0 Assay for 384 Samples User Guide, P/N 703154 ■ ■ Axiom 384HT gDNA Sample Prep QRC, P/N 703163 ■ ■ Axiom ® 2.0 Target Prep 384 Samples QRC, P/N 703160 ■ ■ Axiom ® 2.0 Assay for 24 Samples User Guide, P/N 703335 ■ ■ Axiom ® 2.0 Assay for 24 Samples Manual Target Preparation Protocol QRC, P/N 703337 ■ ■ GeneTitan ® MC Protocol for Axiom ® 384HT Array Plate Processing QRC, P/N 703164 ■ ■ Axiom ® Automated Target Preparation with the Affymetrix ® NIMBUS User Guide, P/N 703185 Precautions 1. AXIOM REAGENTS ARE FOR RESEARCH USE ONLY; NOT FOR DIAGNOSTIC PROCEDURES. 2. Avoid microbial contamination, which may cause erroneous results. 3. WARNING: All biological specimens and materials with which they come into contact should be handled as if capable of transmitting infection and disposed of with proper precautions in accordance with federal, state, and local regulations. This includes adherence to the OSHA Bloodborne Pathogens Standard (29 CFR 1910.1030) for blood- derived and other samples governed by this act. Never pipet by mouth. Avoid specimen contact with skin and mucous membranes. 4. CAUTION: Exercise standard precautions when obtaining, handling, and disposing of potentially carcinogenic reagents. 5. Exercise care to avoid cross-contamination of samples during all steps of this procedure, as this may lead to erroneous results. 6. Use powder-free gloves whenever possible to minimize the introduction of powder particles into sample or array cartridges. Axiom 2.0 Reagent Kit Components The Axiom 96 and 384 reaction reagent kits consist of 4 modules. The 4x24 reaction reagent kit consists of 5 modules. Module 1: P/N 901711 Component 4x24/96 Rxn 384 Rxn Module 1: store at –25°C to –15°C 1 2 ■ ■ Axiom 2.0 Denat Soln 10X ■ ■ Axiom 2.0 Neutral Soln ■ ■ Axiom Water ■ ■ Axiom Reference gDNA 103 ■ ■ Axiom 2.0 Amp Soln ■ ■ Axiom 2.0 Amp Enzyme ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 2 ■ ■ 2 ■ ■ 2 ■ ■ 2 ■ ■ 2 ■ ■ 2 Module 2—Pouch 1 of 2: P/N 901528 Component 4x24/96 Rxn 384 Rxn Module 2—Pouch 1 of 2: store at –25°C to –15°C 1 2 ■ ■ Axiom Frag Enzyme ■ ■ Axiom 10X Frag Buffer ■ ■ Axiom Precip Soln 2 ■ ■ Axiom Hyb Buffer ■ ■ Axiom Hyb Soln 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 1 ■ ■ 2 ■ ■ 2 ■ ■ 2 ■ ■ 2 ■ ■ 2 Axiom ® 2.0 Reagent Kit
Transcript of Precautions AXIOM REAGENTS ARE FOR RESEARCH ......Module 3: Wash Buffer A P/N 901446, Wash Buffer B...
PackageInsert
For Research Use OnlyNot for use in diagnostic procedures.
Intended UseThe Affymetrix Axiom® 2.0 Reagent Kit enables genotyping of SNPs and in/dels on Axiom® Genome-Wide Array Plates and Axiom® myDesign™ Array Plates. This kit is available in a 96, 384, and 4x24 reaction format. The kits include the reagents and control DNA (human) required to:
■■ Produce hybridization-ready targets for Axiom Genome-Wide Array Plates and Axiom myDesign Array Plates.
■■ Process array plates on the Affymetrix GeneTitan® Multi-Channel Instrument.
This product is for research purposes only and is not intended for use in the diagnosis of disease.
Ordering InformationReagent Kits
Product Name Description P/N
Axiom® 2.0 Reagent Kit Sufficient for one 96 array plate 901758
Axiom® 2.0 384HT Reagent Kit Sufficient for one 384HT array plate 902245
Axiom® 2.0 Reagent Kit 4x24 Reactions
Sufficient for four 24 array plates 902798
Supporting ProductsFor a complete list of supporting products please visit www.affymetrix.com.
Affymetrix® products can be purchased directly from Affymetrix in the United States, and many European and Asian countries. For all other territories, please view a list of our distribution partners, which can be located at: www.affymetrix.com/site/contact/index.affx.
Instructions for UseFor instructions on using this reagent kit and Axiom Genome-Wide Array Plates and Axiom myDesign Array Plates, refer to the following documentation:
■■ Axiom® 2.0 Assay Automated Workflow User Guide, P/N 702963 ■■ Axiom® 2.0 Assay Manual Workflow User Guide, P/N 702990■■ Axiom® 2.0 Assay Automated Target Prep Protocol QRC, P/N 702962■■ Axiom® 2.0 Manual Target Prep Protocol QRC, P/N 702989■■ GeneTitan® MC Protocol for Axiom 2.0 Array Plate Processing QRC,
P/N 702988■■ Axiom® 2.0 gDNA Sample Prep Protocol QRC, P/N 702987 ■■ Axiom® 2.0 Assay for 384 Samples User Guide, P/N 703154■■ Axiom 384HT gDNA Sample Prep QRC, P/N 703163■■ Axiom® 2.0 Target Prep 384 Samples QRC, P/N 703160■■ Axiom® 2.0 Assay for 24 Samples User Guide, P/N 703335■■ Axiom® 2.0 Assay for 24 Samples Manual Target Preparation Protocol QRC,
P/N 703337■■ GeneTitan® MC Protocol for Axiom® 384HT Array Plate Processing QRC,
P/N 703164■■ Axiom® Automated Target Preparation with the Affymetrix® NIMBUS User
Guide, P/N 703185
Precautions1. AXIOM REAGENTS ARE FOR RESEARCH USE ONLY; NOT FOR DIAGNOSTIC
PROCEDURES.2. Avoid microbial contamination, which may cause erroneous results.3. WARNING: All biological specimens and materials with which they
come into contact should be handled as if capable of transmitting infection and disposed of with proper precautions in accordance with federal, state, and local regulations. This includes adherence to the OSHA Bloodborne Pathogens Standard (29 CFR 1910.1030) for blood-derived and other samples governed by this act. Never pipet by mouth. Avoid specimen contact with skin and mucous membranes.
4. CAUTION: Exercise standard precautions when obtaining, handling, and disposing of potentially carcinogenic reagents.
5. Exercise care to avoid cross-contamination of samples during all steps of this procedure, as this may lead to erroneous results.
6. Use powder-free gloves whenever possible to minimize the introduction of powder particles into sample or array cartridges.
Axiom 2.0 Reagent Kit ComponentsThe Axiom 96 and 384 reaction reagent kits consist of 4 modules. The 4x24 reaction reagent kit consists of 5 modules.
Module 1: P/N 901711
Component 4x24/96 Rxn 384 Rxn
Module 1: store at –25°C to –15°C 1 2
■■ Axiom 2.0 Denat Soln 10X■■ Axiom 2.0 Neutral Soln ■■ Axiom Water■■ Axiom Reference gDNA 103■■ Axiom 2.0 Amp Soln■■ Axiom 2.0 Amp Enzyme
■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1
■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2
Module 2—Pouch 1 of 2: P/N 901528
Component 4x24/96 Rxn 384 Rxn
Module 2—Pouch 1 of 2: store at –25°C to –15°C 1 2
■■ Axiom Frag Enzyme■■ Axiom 10X Frag Buffer■■ Axiom Precip Soln 2■■ Axiom Hyb Buffer■■ Axiom Hyb Soln 1
■■ 1■■ 1■■ 1■■ 1■■ 1
■■ 2■■ 2■■ 2■■ 2■■ 2
Axiom® 2.0 Reagent Kit
Module 2—Pouch 2 of 2: P/N 901529
Component 4x24/96 Rxn 384 Rxn
Module 2—Pouch 2 of 2: store at 2°C to 8°C 1 2
■■ Axiom Frag Diluent■■ Axiom Frag Rxn Stop■■ Axiom Precip Soln 1■■ Axiom Resusp Buffer■■ Axiom Hyb Soln 2
■■ 1■■ 1■■ 1■■ 1■■ 1
■■ 2■■ 2■■ 2■■ 2■■ 2
Module 3: Wash Buffer A P/N 901446, Wash Buffer B P/N 901447, Axiom Water
P/N 901578
Component 4x24 Rxn 96 Rxn 384 Rxn
Module 3: store at room temperature 4 1 1
■■ Axiom Wash Buffer A■■ Axiom Wash Buffer B■■ Axiom Water
■■ 8■■ 4■■ 4
■■ 2■■ 1■■ 1
■■ 2■■ 1■■ 1
Module 4—Pouch 1 of 2: P/N 901278
Component 4x24/96 Rxn 384 Rxn
Module 4—Pouch 1 of 2: store at –25°C to –15°C 1 2
■■ Axiom Ligate Buffer■■ Axiom Ligate Enzyme■■ Axiom Ligate Soln 1■■ Axiom Probe Mix 1■■ Axiom Stain Buffer■■ Axiom Stabilize Soln
■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1
■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2
Module 4—Pouch 2 of 2: P/N 901276
Component 4x24/96 Rxn 384 Rxn
Module 4—Pouch 2 of 2: store at 2°C to 8°C 1 2
■■ Axiom Ligate Soln 2■■ Axiom Probe Mix 2■■ Axiom Wash A■■ Axiom Stain 1-A■■ Axiom Stain 1-B■■ Axiom Stain 2-A■■ Axiom Stain 2-B■■ Axiom Stabilize Diluent■■ Axiom Water■■ Axiom Hold Buffer
■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1■■ 1
■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2■■ 2
Module 5—Pouch 1 of 2: P/N 902796 (4x24 Reaction Kit only)
Component 4x24 Rxn
Module 5—Pouch 1 of 2: store at –25°C to –15°C 1
■■ Axiom Ligate Enzyme ■■ 1
Module 5—Pouch 2 of 2: P/N 902797 (4x24 Reaction Kit only)
Component 4x24 Rxn
Module 5—Pouch 2 of 2: store at 2°C to 8°C 1
■■ Axiom Hold Buffer ■■ 3
Axiom® 2.0 Reagent Kit P/N 702964 Rev. 3
Safety InformationA Safety Data Sheet(s) (SDS) is available at www.affymetrix.com.
If the product is a kit or is supplied with more than one material, please refer to the SDS for each component for hazard information. The SDS are also available at: www.affymetrix.com/support/technical/safetydatasheetsmain.affx
Caution:The 10X Denaturation Solution contains Potassium Hydroxide. Warning: Corrosive [CAS# 1310-58-3]
The Hybridization Buffer contains Tetramethylammonium Chloride (TMAC). Warning: Highly Toxic. [CAS# 75-57-0]
The Hybridization Solution 2 contains Formamide. Warning: Teratogen. [CAS# 75-12-7]
The Stabalize Solution contains Glutaraldehyde. Warning: Sensitizer. [CAS# 111-30-8]
The Ligate Solution 2 contains Dimethyl Sulfoxide (DMSO). Warning: Combustible Liquid. [CAS# 67-68-5]
The Wash Buffer A and Wash Buffer B contain ≤ 0.02% Sodium Azide. [CAS# 26628-22-8]
Limited LicenseAffymetrix hereby grants to buyer a non-exclusive, non-transferable, non-sublicensable license to Affymetrix’ Core Product IP to use the product(s), but only in accordance with the product labels, inserts, manuals and written instructions provided by Affymetrix. “Core Product IP” is the intellectual property owned or controlled by Affymetrix as of the shipment date of a product that covers one or more features of the product that are applicable in all applications of the product that are in accordance with the product labels, inserts, manuals and written instructions provided by Affymetrix. The license granted herein to buyer to the Core Product IP expressly excludes any use that: (i) is not in accordance with the product labels, inserts, manuals and written instructions provided by Affymetrix, (ii) requires a license to intellectual property that covers one or more features of a product that are only applicable within particular fields of use or specific applications, (iii) involves reverse engineering, disassembly, or unauthorized analysis of the product and/or its methods of use, or (iv) involves the re-use of a consumable product. Buyer understands and agrees that except as expressly set forth, no right or license to any patent or other intellectual property owned or controlled by Affymetrix is granted upon purchase of any product, whether by implication, estoppel or otherwise. In particular, no right or license is conveyed or implied to use any product provided hereunder in combination with a product or service not provided, licensed or specifically recommended by Affymetrix for such use. Furthermore, buyer understands and agrees that buyer is solely responsible for determining whether buyer possesses all intellectual property rights that may be necessary for buyer’s specific use of the product, including any rights from third parties.
Contact Information
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Please visit our web site for international distributor contact information www.affymetrix.com
TrademarksAffymetrix®, Axiom®, GeneChip®, Command Console®, CytoScan®, DMET™, Eureka™, Eureka Genomics®, Eureka Genotyping™, Expression Console™ GeneAtlas®, GeneChip-compatible™, GeneTitan®, Genotyping Console™, myDesign™, MyGeneChip™, NetAffx®, OncoScan®, Powered by Affymetrix™, PrimeView®, and ViewRNA® are trademarks or registered trademarks of Affymetrix, Inc. Please see www.affymetrix.com/trademarks for a complete list of Affymetrix trademarks. All other trademarks are the property of their respective owners.
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