PPT Defense Salmonella
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Transcript of PPT Defense Salmonella
Prevalence of multi-drug resistant Salmonella Typhi in street foods in Dasmarias, Cavite
Presented by: John Royce Gnilo Mark Sumague
Typhoid Fever Affects 20 million people
Water or food-borne disease Caused by Salmonella Typhi Endemic in poor countries
Cases of typhoid in the Philippines
Number of Cases
2005
2006
2007
2008
2009
Fig. 1 Distribution of typhoid cases by year Philippines, 2005-2009
Fig 2. Infected areas in the Philippines
Numbe r of Cases
Fig. 3 Distribution of typhoid cases by month Philippines
SourcesFoods can be contaminated by bacteria due to improper sanitary procedures
Meat
egg
Poultry
fish products (Rheinlander et al. 2008)
Prevention and Treatment Sanitary measures
Personal hygiene Antibiotic therapy
Drug resistant Resistant to Ampicillin, Chloramphenicol and
cotrimoxazole (Akinyemi et al. 2004). Resistant occurs because they may
accumulate many genes Each coding for resistance to a single drug, within the cell (Nikaido 2009).
Objective Determine the occurrence of multi-drug resistant
Salmonella Typhi in street foods in Dasmarias, Cavite
Statement of the problemWhat is the prevalence of Salmonella in selected street foods and food stalls in Dasmarias Cavite? 2. Are culturable and non-culturable Salmonella Typhi present in selected street foods? 3. Is there a difference between the culturable and non culturable Salmonella Typhi in relation to type of street foods? 4. Is multi-drug resistant Salmonella Typhi present in street foods?1.
Scope and limitations Scope: 1. Food samples 2. Detection of Salmonella Typhi in street foods 3. Determination of drug resistance 4. Molecular detection of Salmonella Typhi 5. drug resistant pattern
1. 2. 3.
LimitationsS. Typhi will only be observed stn gene Antibiotics Street foods that can only be found in 5 stalls
4.
Significance of the study Local Government Unit (LGU)
Public Student researchers
METHODOLOGY
Research Design Cross sectional analysis
Total of 50 samples
Research Setting
Biological Sciences Department Research Laboratory
De La Salle Health Sciences and Institute
De La Salle University- Dasmarias
Sample Collection
Isolation of SalmonellaHomogenization broth incubate at 37C for 18 hrs Streak plating in SSA
Observation
incubate at 37C for 24 hrs
Enrichment 24 hrs Colonies Antimicrobial Susceptibility Assay
centrifuge Genomic DNA kit Enriched sample
PCR
Gel Electrophoresis
Data Gathering and Statistical AnalysisTable 1. Cross-sectional analysisSalmonella (+,-)stall sample replication culturable 1 1 1 2 2 1 2 3 1 2 4 1 2 5 1 2 non culturable Salmonella Typhi (+,-)
Note : Same procedure in stalls 2,3,4 and 5.
Table 2. Multi-drug resistance of the isolatesAntiobiotic: Sensitive or Resistant ( less than 14 mm ) isolates ampicillin chloramphenicol cotrimoxazole streptomycin tetracyline
1
2
3
4
5
Note: 30 isolates will be conducted
Statistical Analysis ANOVA
STATA 9.0 p