PPSQ-31A / 33A - Shim-pol€¦ · C297-E055B PPSQ-31A / 33A Protein Sequencer PPSQ-31A / 33A...
Transcript of PPSQ-31A / 33A - Shim-pol€¦ · C297-E055B PPSQ-31A / 33A Protein Sequencer PPSQ-31A / 33A...
C297-E055BPPSQ-31A
/ 33A
Protein Sequencer
PPSQ-31A / 33A
Printed in Japan 3655-05318-30AMF
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
© Shimadzu Corporation, 2013www.shimadzu.com/an/
Achieving Greater Simplicity and Reliabil ity in the Determination of Amino Acid Sequences
Repeated Use of Mobile PhaseReduced running costs and l iquid waste in theanalysis of PTH-amino acids using isocratic mode
Software with Excellent Operabil itySimple operations andeasy-to-use data analysis functions
Analytical Stabil ityExcellent baseline stabil ity andretention time reproducibil ity
Protein Sequencer
PPSQ-31A / 33AP. 4
P. 5
P. 5
Achieving Greater Simplicity and Reliabil ity in the Determination of Amino Acid Sequences
Repeated Use of Mobile PhaseReduced running costs and l iquid waste in theanalysis of PTH-amino acids using isocratic mode
Software with Excellent Operabil itySimple operations andeasy-to-use data analysis functions
Analytical Stabil ityExcellent baseline stabil ity andretention time reproducibil ity
Protein Sequencer
PPSQ-31A / 33AP. 4
P. 5
P. 5
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
mV
D
E
TH
N Q S
G AY R
M VP W
F
K
I L
PTH-AA
2D Electrophoresis Image of Proteins Extracted from E. Coli
10pmol
Spot 1
Sequence analysis can be started by simply registering
the number of analysis cycles, data file names, and other
information, and then clicking the [Start] button.
When a reagent is replaced, the inside of the reagent bottle is
purged with nitrogen. The replacement dates and times of the
reagents can be ascertained with a glance, and lot control is
possible.
Analytical Stabil ity
Baseline Stabil ity
Repeated Use of Mobile Phase
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Software with Excellent Operabil ity
Simple OperationsSpecialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Main Window
Sequence Schedule Window Reagent Control Window
Retention Time Reproducibil ity
Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
cycle 1 cycle 2
cycle 14 cycle 15
cycle 24 cycle 25
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
DTT
S
DMPTU DPTU
DPU DTT
T
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
DMPTU
LDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
Y
DTT DPTU
DMPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
EDTT
DMPTUDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50mV
K
DTT
DMPTUDPTU
Selection of sequencing schedule
Summary display of currently executed cycle and step and estimated completion time
4 5PPSQ-31A / 33A
Protein Sequencer
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
mV
D
E
TH
N Q S
G AY R
M VP W
F
K
I L
PTH-AA
2D Electrophoresis Image of Proteins Extracted from E. Coli
10pmol
Spot 1
Sequence analysis can be started by simply registering
the number of analysis cycles, data file names, and other
information, and then clicking the [Start] button.
When a reagent is replaced, the inside of the reagent bottle is
purged with nitrogen. The replacement dates and times of the
reagents can be ascertained with a glance, and lot control is
possible.
Analytical Stabil ity
Baseline Stabil ity
Repeated Use of Mobile Phase
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Software with Excellent Operabil ity
Simple OperationsSpecialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Main Window
Sequence Schedule Window Reagent Control Window
Retention Time Reproducibil ity
Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
cycle 1 cycle 2
cycle 14 cycle 15
cycle 24 cycle 25
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
DTT
S
DMPTU DPTU
DPU DTT
T
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.0
0.2
0.4
0.6
0.8
1.0 mV
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
DMPTU
LDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
Y
DTT DPTU
DMPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50 mV
EDTT
DMPTUDPTU
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min
0.00
0.10
0.20
0.30
0.40
0.50mV
K
DTT
DMPTUDPTU
Selection of sequencing schedule
Summary display of currently executed cycle and step and estimated completion time
4 5PPSQ-31A / 33A
Protein Sequencer
Reprocessing of chromatograms required
for data analysisOverlay of multiple
chromatograms
Automatic Sequence Estimation
Automatic estimation of amino acid sequences
Chromatogram peak integration, printing, and other
processes can be performed on a per-sample basis.
Displays multiple chromatograms. Analysis cycles can be
updated simply by clicking a button.
Window allows easy identification of sequences.
Calibrates PTH-amino acids.
Retention times can be edited simply by selecting
a PTH-amino acid and clicking a peak.
Automatically generates a report containing analytical parameters,
estimated sequences, yield rates, and other information.
If the appropriate settings are entered before analysis
starts, sequences are automatically estimated after each
cycle is complete. Up to four candidate amino acids are
displayed together with their certainty levels.
Customized Reports
Reports containing information such as analytical
parameters, estimated sequences, and yield rates are
automatically created.
Yield Graph Display
Initial and repeated yield rates are calculated and
displayed in graph form. Amino acids used for
calculations can be freely selected.
Reaction method
Reaction time
Number of reactors
Sample immobilization method
Reactor temperature control
Converter temperature control
Column temperature control
Number of reagents/solvents
Reagent/solvent delivery method
Dimensions
Weight
Edman degradation
PPSQ-31A: 47 min/cycle
PPSQ-33A: 48.5 min/cycle
PPSQ-31A: 1
PPSQ-33A: 3
Glass fiber disk (8 mm dia.) or PVDF membrane
Room temperature + 10 °C to 60 °C
Room temperature + 10 °C to 70 °C
Room temperature + 10 °C to 60 °C
8 (one is an option)
Nitrogen gas pressure
W650 × D500 × H520 mm
PPSQ-31A: 55 kg
Main Unit
Reagents for amino acid sequence analysis (7 types)
Glass fiber disks
Polybrene reagent
Apomyoglobin standard
Mobile phase for PTH-amino acids
Separation column for PTH-amino acids
Mixed standards for PTH-amino acids
1 of each type
50
1
1
1 L
1
2 mL x 4
Part name QuantityPPSQ-31A/33A
Installation space
Power supply
Nitrogen gas
Exhaust equipment
Desktop: W1,730 × D600 × H550 mm min.
(Weight: Approx. 93 kg)
100 VAC, 1,100 VA, 50/60 Hz
Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing
(10 m) are provided as standard accessories.
In order to provide an exhaust for gases
produced by waste liquids, an exhaust tube
must either be connected to exhaust
equipment or led outside. An exhaust tube
(20 m) is provided as a standard accessory.
Flow rate setting range
Dimensions
Weight
0.001 to 10.000 mL/min
W260 × D420 × H140 mm
11 kg
Solvent Delivery Module LC-20AT
292-31310-XX
292-05684-92
PPSQ-31A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
Wavelength setting range
Dimensions
Weight
190 to 700 nm
W260 × D420 × H140 mm
13 kg
Detector SPD-20A
Computer
Display
Note: In the interests of product improvement, these specifications may change without notice.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.
Note: The required installation space and power supply may change according to the PC and display used.
Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.
Offers highly cost-effective performance.
PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)
IBM PC/AT or compatible
OS: Windows® XP, Windows 7
17-inch LCD
Control PC
Standard Configurations
Startup Kit
Other Items Provided by User
PPSQ-31A System
292-31330-XX
292-05684-92
PPSQ-33A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
PPSQ-33A System
PPSQ-31A Single Reactor
Specifications
Simple, Easy-to-Use Data Analysis FunctionsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.
6 7PPSQ-31A / 33A
Protein Sequencer
Reprocessing of chromatograms required
for data analysisOverlay of multiple
chromatograms
Automatic Sequence Estimation
Automatic estimation of amino acid sequences
Chromatogram peak integration, printing, and other
processes can be performed on a per-sample basis.
Displays multiple chromatograms. Analysis cycles can be
updated simply by clicking a button.
Window allows easy identification of sequences.
Calibrates PTH-amino acids.
Retention times can be edited simply by selecting
a PTH-amino acid and clicking a peak.
Automatically generates a report containing analytical parameters,
estimated sequences, yield rates, and other information.
If the appropriate settings are entered before analysis
starts, sequences are automatically estimated after each
cycle is complete. Up to four candidate amino acids are
displayed together with their certainty levels.
Customized Reports
Reports containing information such as analytical
parameters, estimated sequences, and yield rates are
automatically created.
Yield Graph Display
Initial and repeated yield rates are calculated and
displayed in graph form. Amino acids used for
calculations can be freely selected.
Reaction method
Reaction time
Number of reactors
Sample immobilization method
Reactor temperature control
Converter temperature control
Column temperature control
Number of reagents/solvents
Reagent/solvent delivery method
Dimensions
Weight
Edman degradation
PPSQ-31A: 47 min/cycle
PPSQ-33A: 48.5 min/cycle
PPSQ-31A: 1
PPSQ-33A: 3
Glass fiber disk (8 mm dia.) or PVDF membrane
Room temperature + 10 °C to 60 °C
Room temperature + 10 °C to 70 °C
Room temperature + 10 °C to 60 °C
8 (one is an option)
Nitrogen gas pressure
W650 × D500 × H520 mm
PPSQ-31A: 55 kg
Main Unit
Reagents for amino acid sequence analysis (7 types)
Glass fiber disks
Polybrene reagent
Apomyoglobin standard
Mobile phase for PTH-amino acids
Separation column for PTH-amino acids
Mixed standards for PTH-amino acids
1 of each type
50
1
1
1 L
1
2 mL x 4
Part name QuantityPPSQ-31A/33A
Installation space
Power supply
Nitrogen gas
Exhaust equipment
Desktop: W1,730 × D600 × H550 mm min.
(Weight: Approx. 93 kg)
100 VAC, 1,100 VA, 50/60 Hz
Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing
(10 m) are provided as standard accessories.
In order to provide an exhaust for gases
produced by waste liquids, an exhaust tube
must either be connected to exhaust
equipment or led outside. An exhaust tube
(20 m) is provided as a standard accessory.
Flow rate setting range
Dimensions
Weight
0.001 to 10.000 mL/min
W260 × D420 × H140 mm
11 kg
Solvent Delivery Module LC-20AT
292-31310-XX
292-05684-92
PPSQ-31A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
Wavelength setting range
Dimensions
Weight
190 to 700 nm
W260 × D420 × H140 mm
13 kg
Detector SPD-20A
Computer
Display
Note: In the interests of product improvement, these specifications may change without notice.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.
Note: The required installation space and power supply may change according to the PC and display used.
Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.
Offers highly cost-effective performance.
PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)
IBM PC/AT or compatible
OS: Windows® XP, Windows 7
17-inch LCD
Control PC
Standard Configurations
Startup Kit
Other Items Provided by User
PPSQ-31A System
292-31330-XX
292-05684-92
PPSQ-33A (main unit)
LC-20AT
SPD-20A
PPSQ-30 (control PC set)
Startup kit
Part number Part name
PPSQ-33A System
PPSQ-31A Single Reactor
Specifications
Simple, Easy-to-Use Data Analysis FunctionsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.
6 7PPSQ-31A / 33A
Protein Sequencer
C297-E055BPPSQ-31A
/ 33A
Protein Sequencer
PPSQ-31A / 33A
Printed in Japan 3655-05318-30AMF
Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.
For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.
© Shimadzu Corporation, 2013www.shimadzu.com/an/