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The Effect of Ginseng

on Lumbriculusvariegatus

Nicole Loughry, Johnna Price,

and Kayanna Sayre

HCOP PE 2008


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Ginseng Facts

History

Antioxidant

Ginsenosides

Dose/response relationship


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Pulsation Rate:

Hypothesis

Ginseng will lower the pulsation ratein Lumbriculus variegatus.


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Experimental DesignPulsation Rate

Control

(SpringWater)

Treatment1(Ginseng12g%)

Treatment

2(Ginseng24g%)

12 worms12 worms

12 worms

6 worms10

minutes

6 worms10

minutes

6 worms10

minutes

6 worms20

minutes

6 worms20

minutes

6 worms20

minutes


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Materials and Methods

N= 12 worms in 1 mL spring water (controlgroup)

N= 12 worms in 1 mL 12g% ginseng

(treatment group 1) N=12 worms in 1 mL 24g% ginseng

(treatment group 2)

Exposed 6 worms from each group inginseng for 10 minutes

Exposed 6 worms from each group inginseng for 20 minutes


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Materials and MethodsContinued

Individually removed worms and countedthe pulsation rates underneath amicroscope for 10 seconds and multipliedby 6 to get beats per minute.

Performed ANOVA (analysis of variance)

Model variables were treatment, time, andtreatment*time

Mean comparisons were made using

Students t-test


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Treatment

p=


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Hypothesis-

Locomotion

Ginseng will increase locomotion inLumbriculus variegatus.


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Experimental DesignLocomotion

Control(SpringWater) Treatment1

(Ginseng6g%)

Treatment

2(Ginseng12g%)

12 worms12 worms

12 worms6

worms6 p/m

6worms12 p/m 6

worms

6

worms

6worms

6 p/m

6worms

12 p/m

p/m=probes

per minute


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Material and MethodsLocomotion

N= 12 worms in 1 mL spring water (controlgroup)

N= 12 worms in 1 mL 12g% ginseng(treatment group 1)

N=12 worms in 1 mL 24g% ginseng(treatment group 2)

Constructed a race track for the worms

Exposed 12 worms in each solution for tenminutes

Place one worm on the track and probed

every 5 seconds for one minute and
http://www.youtube.com/watch?v=AjCFCcHTLJghttp://www.youtube.com/watch?v=AjCFCcHTLJg


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Statistical Analysis

Calculated mean and standard deviation

Performed ANOVA (analysis of variance)

Model variables were treatment, time, andtreatment*time

Mean comparisons were made usingStudents t-test

Data re orted as means and standard


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Treatment

p=0.9980

Time

p=0.89345 seconds


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Chemotaxis Hypothesis

Ginseng will produce a negativechemotactic response in Lumbriculusvariegatus.


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Experimental Design

Chemotaxis

Control(springwater)

Treatment2

(ginseng12g%)

20worms

20worms

Treatment1

(ginseng

6g%)

20worms


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Materials and Methods

N=20 worms, individually, added one dropof spring water at tail and observedreaction

N=20 worms, individually, added one dropof 6 g% ginseng and observed reaction

N=20 worms, individually, added one dropof 12 g% ginseng and observed reaction

Recorded data


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Chemotactic Test Results


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Conclusion

In conclusion, when Lumbriculusvariegatus are subjected to a lowconcentration (12 g%) for 10 minutes,the pulsation rate increases. In support ofour hypothesis, when exposed to 24 g%ginseng, pulsation rate decreased.

Contrary to our hypothesis, ginseng did

not affect the locomotion of Californiablackworms.

Contrary to our hypothesis, ginsengproduced a positive chemotactic response.


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Discussion

Although ginseng is prized for itsantioxidant benefits, it appears tohave stimulatory effects on thevasculature of worms. More researchis needed to determine if this is truein humans.


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Works Cited Drewes, Charles PhD. Those wonderful worms.

Lumbriculus variegatus Lab. Department ofZoology and Genetics University of Iowa.Accessed 2008 June 18.

[Anonymous]. Ginseng: the mystical healer[Internet]. Disabled World web site. October 23,2006. Accessed 2008 June 18.

http://kmsayre.blogspot.com

http://nlloughry.blogspot.com

http://johnnaprice.blogspot.com

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