Posters - Affiches - Foundation · Presenter Institution Title of Project Poster Number...

Presenter Institution Title of Project Poster NumberPrésentateur Établissement Titre du projet Numéro d'affiche

Allain Eric Université de Moncton Investigation of the mechanisms by whichalternatively-spliced 5-lipoxygenase isoformsinhibit leukotriene biosynthesis

64

Arsenault Bishop Nicole Université de Moncton Dietary intake of DHA and EPA in a group ofpregnant women in the Moncton area

57

Beauregard Annie-Pier Université de Moncton 3'UTR polymorphism and miRNA regulationof PAX5 expression in cancer cells

8

Belkaid Anissa Université de Moncton 17β-estradiol-induced breast cancer cell proliferation requires stearoyl-CoAdesaturase-1 expression and activity

48

Benzina Sami Université de Moncton Pax-5 regulates breast cancer malignancythrough the disruption of FAK1 signaling

7

Bjurman Natalie Mount AllisonUniversity

The risk of Borrelia infection (Lyme disease)in New Brunswick - using dogs as a sentinelspecies to assess the risk to humans"

37

Bos Allyson University of NewBrunswick, Saint John

Canadian medicinal plants… a source ofanticancer drug leads?

16

Boudreau Corinne Mount AllisonUniversity

Experiences with chronic undiagnosed illnessin Canada

41

Bouhamdani Nadia Université de Moncton Proteomic approaches to study a smallmolecule targeting kidney cancer

25

Brodeur Nathalie N. Université de Moncton Mind the Heart: Best Practices forPrevention, Early Identification andTreatment of Mood and Anxiety Disorders inMen with Heart Disease

4

Bullerwell Charles E. Atlantic CancerResearch Institute

Characterization of the Pax5 interactome:PARP-1 can influence B-cell-specific geneexpression

51

Bulman Donna University of NewBrunswick

Men’s health promotion and workengagement in the context of workplacebullying.

13

Cahuzac Maxime Université de Moncton Inhibition des lysosomes et modulation dumétabolisme de la sphingosine pour cibler lescellules cancéreuses rénales

23

Chaulk Mitchell University of NewBrunswick

On the Feasibility of Using PatternRecognition Based Myoelectric Control as aHuman-Computer Interface for Individualswith Paralysis

24

Clark Trevor N. UNBSJ Metabolomic approaches to increasing theefficiency of natural products for drugdiscovery

10

Cloutier Frank CHU-DUMONT A new surgical approach that usepolypropylene tapes for gynecologicalprolapse repair

61

Comeau Dominique Université de Moncton Characterization of Novel miRNAs Involved inBreast Cancer Metastasis

38

Posters - Affiches


Complak Kathleen University of NewBrunswick, Saint John

Barking up the right tree 20

Cormier Jeremy Université de Moncton Effect of a supramaximal exercise on FFAlevels in obese adolescent boys

18

Cote Claudia Dalhousie MedicineNew Brunswick

Effects of Transcatheter Aortic ValveImplantation on Cognitive Function

72

Crapoulet Nicolas Atlantic CancerResearch Institute

Validation of two next-generation sequencingcancer panels for clinical testing

40

Daigle Mikella Université de Moncton Expressions of Sox5 and Sox13 transcriptionfactors are increased in testicular Leydig cellsof mice during postnatal development/ Lesexpressions des facteurs de transcriptionSox5 et Sox13 augmentent dans les cellulesde Leydig du testicule murin lors dudéveloppement postnatal

62

Deprez Pierre Institut Atlantique deRecherche sur leCancer

Synthetic Lethality Screen of TumorSuppressor Pathways for TherapeuticCompound Development

63

Desnoyers Guillaume Atlantic CancerResearch Institute

Decreased eIF3e expression can mediateepithelial-to-mesenchymal transition throughactivation of the TGF β signaling pathway

45

Doucet Étienne Université de Moncton Is the province of New Brunswick limited interms of physical activity resources? A pilotstudy

29

D'Souza Kenneth Dalhousie MedicineNew Brunswick

Regulation of autotaxin in hypertrophiccardiomyopathy

27

Duguay Sabrina Université de Moncton SCD-1 activation in prostate cancer cells byandrogens

52

Eadie Ashley Dalhousie MedicineNew Brunswick

The Role of Heme Regulatory Enzymes inMyocytes under Stress

55

Flewelling Andrew J University of NewBrunswick, Saint John

Can pictures avoid redundancy? The use ofbioactivity profiling to prioritise endophytenatural product isolations

15

Garcés Dávila Isabel University of NewBrunswick, Fredericton

Accountability of Mental Health Services innon-Profit Organizations: Examining the Linkbetween Mandates and Outcomes in NewBrunswick

32

Girouard Gabriel Réseau de santéVitalité

Introduction du dosage STAT de laprocalcitonine au Centre hospitalieruniversitaire Dr-Georges-L.-Dumont -Introduction of a STAT procalcitonin assay atthe Dr. Georges-L.-Dumont UniversityHospital Centre

22

Gould Odette Mount AllisonUniversity

Medication adherence in university students 12

Graves Monica Dalhousie University A work in progress: Perceived barriers andenablers held by interprofessional teammembers around collaborating whencommunicating through the use ofinformation and communication technologies

71


Guerrette Roxann Université de Moncton Functional characterization of Mammaglobin-1 in breast cancer aggressiveness

1

Hamilton-Wright Andrew Mount AllisonUniversity

Determining the utility of mobile applicationsfor managing blood-iron in hemochromatosistreatment

43

Handrigan Grant Université de Moncton Childhood obesity affects balance control andaiming performance

54

Harquail Jason Université de Moncton MiRNAs are important regulators of Pax-5associated breast cancer processes

3

Harris Kami Mount AllisonUniversity

Assessing quantitative PCR and nested PCRprotocols for detection of Borrelia burgdorferi,the Lyme disease bacteria

33

Hassan Ansar CardiovascularResearch NewBrunswick

The Impact of Geographic Factors on Accessto Cardiac Catheterization in New Brunswick

30

Hussaini Ali University of NewBrunswick - IBME

Powered Wrist Motion - Independent WristMotion for Transradial MyoelectricProstheses

11

Iancu Horia-Daniel School of Kinesiologyand Leisure - U de M

Effects of high intensity exercise training onanaerobic and aerobic contributions to totalenergy release during repeatedsupramaximal exercise in obese adults

28

Jabbour Georges Université de Moncton Effect of Low Frequency NeuromuscularElectrical Stimulation on Glucose profile ofPersons with Type 2 Diabetes: A pilot study

19

Jbilou Jalila Université de Moncton Supporting Men Engaging in Positive HealthyPractices in New-Brunswick

2

Jean Jonathan Stan Cassidy Centrefor Rehabilitation

Amyotrophic Lateral Sclerosis Incidence andCluster Identification in New Brunswick,Canada, over a 10-Year Period

58

Kemp Michael Vitalité Health Network A Naturalistic Study of Paliperidone Palmitatein Patients with Schizophrenia: Methods ofthe Canadian Sustenna TreatmentAssessment Registry (CanSTAR)

44

Kuruganti Usha University of NewBrunswick

The Development of an Isokinetic Adapter forProsthesis Users

5

LeBlanc-Kwaw Denise University of NewBrunswick

Effects of workplace bullying on men 14

Lewis Julie Mount AllisonUniversity

Assessing the risk of Lyme borreliosis in NewBrunswick

50

Li Haoxin University of NewBrunswick

Can microarray decipher theantimycobacterial mode of action of C17diynes?

9

MacIntosh Judith University of NewBrunswick


13



MacKenzie Jason U de Sherbrooke Ressemblance entre les pratiques de diverstypes d’activité physique chez des enfants en5e et 6e année et leurs parents

66


McCullum Shane Stan Cassidy Centrefor Rehabilitation

A One Year Pilot Study of Circuit ClassOutcomes in Outpatient NeurologicalPopulation

60

McGibbon Chris University of NewBrunswick

Instrumented pendulum test for objectivespasticity assessment of the knee

42

Melville Sarah Dalhousie MedicineNew Brunswick

Assessment of the Non-Invasive PulsewaveHealth Monitor^TM for Blood PressureDetermination in a Clinical Setting ofCardiovascular Catheterization/ Évaluation del’appareil non-invasive Pulsewave HealthMonitor^TM pour la détermination de lapression artérielle dans une clinique decathétérisation cardiaque

67

Melvin Kathryn University of NewBrunswick, Saint John

Variation of the amount of falcarindiol, anantimycobacterial natural product, within theCanadian medicinal plant Heracleummaximum

17

Mohamed Abeer University of NewBrunswick

Modeling and simulation of transfemoralAmputee gait

26

Patterson Jamie Mount AllisonUniversity

Assessing the potential of Ixodes cookei as avector for Lyme Disease

39

Paulin Anne Université de Moncton L'entrainement par exercice supramaximalrépété améliore la contribution lipidique à lafourniture énergétique lors d'un testincrémental chez des adultes obèses

53

Pelletier-Hibbert Maryse Faculty of Nursing,University of NewBrunswick

Husbands Living with Women on Dialysis:Embracing Their Transformed Life

6

Philip Shona Dalhousie MedicineNew Brunswick

Nanoparticle delivery of curcumin to humanendothelial cells demonstrates efficientuptake and no overt toxicity to signficantlyinduce the expression of heme-oxygenase-1

68

Pulinilkunnil Thomas Dalhousie MedicineNew Brunswick

Angiotensin II acutely increases lysosomalautophagy in the murine myocardium

70

Robart Tess Dalhousie MedicineNew Brunswick

A subcellular fractionation method to enrichendoplasmic reticulum from Saccharomycescerevisiae and human fibroblasts with ALG9mutation

36

Robichaud Sarah Université de Moncton Contribution of the glutamine/glutamate cyclein response to STF-62247 revealed bymetabolic profiling : Correlation between thePI3K/AKT/FOXO pathway and autophagy

49

Roy Patrick Université de Moncton -Campus de Moncton

Effet de l'antagonisme des récepteursnicotiniques sur les monocytes

65

Slade Logan University of NewBrunswick, Saint John

Dieldrin augments mTOR signaling andinhibits autophagy in the adult zebrafish heart(Danio rerio)

59

Sproul Morgan UNBSJ Change the recipe, change the dish 21Stewart Samantha University of New

Brunswick

Food related worries and body image issuesin student athletes: A photovoice exploration.

35


Thompson Ashley University of NewBrunswick

Comparing Access to Primary Health Careamong New and Traditional Models:

31

Touaibia Mohamed Université de Moncton Antihypertensive effect of caffeic acidanalogs through dual renin-angiotensin-aldosterone system inhibition

34

TRIVEDI PURVI Dalhousie MedicineNew Brunswick

Diet induced obesity and type 2 diabetesimpairs lysosomal autophagy in the murinemyocardium

46

Wall Michael Atlantic CancerResearch Institute

HNRNP H IS A NOVEL STRESS GRANULE-ASSOCIATED PROTEIN AND ASSISTS INCELLULAR STRESS RECOVERY

56

Wang Hao Government of NewBrunswick

Trends in incidence and prevalence of heartfailure in New Brunswick

47

Ward Stephanie Université deSherbrooke

Influence of childcare educators on physicalactivity and eating behaviours of preschool-aged children: A systematic review

69

Zwicker Alyson Dalhousie MedicineNew Brunswick


36


Guerrette Roxann Université de Moncton Functional characterization of Mammaglobin-1 in breast cancer aggressiveness

1

Jbilou Jalila Université de Moncton Supporting Men Engaging in Positive HealthyPractices in New-Brunswick

2

Harquail Jason Université de Moncton MiRNAs are important regulators of Pax-5associated breast cancer processes

3

Brodeur Nathalie N. Université de Moncton Mind the Heart: Best Practices for Prevention,Early Identification and Treatment of Moodand Anxiety Disorders in Men with HeartDisease

4

Kuruganti Usha University of NewBrunswick

The Development of an Isokinetic Adapter forProsthesis Users

5

Pelletier-Hibbert Maryse Faculty of Nursing,University of NewBrunswick

Husbands Living with Women on Dialysis:Embracing Their Transformed Life

6

Benzina Sami Université de Moncton Pax-5 regulates breast cancer malignancythrough the disruption of FAK1 signaling

7

Beauregard Annie-Pier Université de Moncton 3'UTR polymorphism and miRNA regulation ofPAX5 expression in cancer cells

8

Li Haoxin University of NewBrunswick

Can microarray decipher theantimycobacterial mode of action of C17diynes?

9

Clark Trevor N. UNBSJ Metabolomic approaches to increasing theefficiency of natural products for drugdiscovery

10

Hussaini Ali University of NewBrunswick - IBME

Powered Wrist Motion - Independent WristMotion for Transradial Myoelectric Prostheses

11

Gould Odette Mount Allison University Medication adherence in university students 12

Bulman Donna University of NewBrunswick


13


13



LeBlanc-Kwaw Denise University of NewBrunswick

14

Flewelling Andrew J University of NewBrunswick, Saint John

Can pictures avoid redundancy? The use ofbioactivity profiling to prioritise endophytenatural product isolations

15

Bos Allyson University of NewBrunswick, Saint John

Canadian medicinal plants… a source ofanticancer drug leads?

16

Posters - Affiches


Melvin Kathryn University of NewBrunswick, Saint John

Variation of the amount of falcarindiol, anantimycobacterial natural product, within theCanadian medicinal plant Heracleummaximum

17

Cormier Jeremy Université de Moncton Effect of a supramaximal exercise on FFAlevels in obese adolescent boys

18

Jabbour Georges Université de Moncton Effect of Low Frequency NeuromuscularElectrical Stimulation on Glucose profile ofPersons with Type 2 Diabetes: A pilot study

19

Complak Kathleen University of NewBrunswick, Saint John

Barking up the right tree 20

Sproul Morgan UNBSJ Change the recipe, change the dish 21Girouard Gabriel Réseau de santé

Vitalité

Introduction du dosage STAT de laprocalcitonine au Centre hospitalieruniversitaire Dr-Georges-L.-Dumont -Introduction of a STAT procalcitonin assay atthe Dr. Georges-L.-Dumont UniversityHospital Centre

22

Cahuzac Maxime Université de Moncton Inhibition des lysosomes et modulation dumétabolisme de la sphingosine pour cibler lescellules cancéreuses rénales

23

Chaulk Mitchell University of NewBrunswick

On the Feasibility of Using PatternRecognition Based Myoelectric Control as aHuman-Computer Interface for Individualswith Paralysis

24

Bouhamdani Nadia Université de Moncton Proteomic approaches to study a smallmolecule targeting kidney cancer

25

Mohamed Abeer University of NewBrunswick

Modeling and simulation of transfemoralAmputee gait

26

D'Souza Kenneth Dalhousie MedicineNew Brunswick

Regulation of autotaxin in hypertrophiccardiomyopathy

27

Iancu Horia-Daniel School of Kinesiologyand Leisure - U de M

Effects of high intensity exercise training onanaerobic and aerobic contributions to totalenergy release during repeated supramaximalexercise in obese adults

28

Doucet Étienne Université de Moncton Is the province of New Brunswick limited interms of physical activity resources? A pilotstudy

29

Hassan Ansar CardiovascularResearch NewBrunswick

The Impact of Geographic Factors on Accessto Cardiac Catheterization in New Brunswick

30

Thompson Ashley University of NewBrunswick

Comparing Access to Primary Health Careamong New and Traditional Models:

31

Garcés Dávila Isabel University of NewBrunswick, Fredericton

Accountability of Mental Health Services innon-Profit Organizations: Examining the Linkbetween Mandates and Outcomes in NewBrunswick

32

Harris Kami Mount Allison University Assessing quantitative PCR and nested PCRprotocols for detection of Borrelia burgdorferi,the Lyme disease bacteria

33

Touaibia Mohamed Université de Moncton Antihypertensive effect of caffeic acid analogsthrough dual renin-angiotensin-aldosteronesystem inhibition

34


Stewart Samantha University of NewBrunswick

Food related worries and body image issuesin student athletes: A photovoice exploration.

35

Zwicker Alyson Dalhousie MedicineNew Brunswick


36

Robart Tess Dalhousie MedicineNew Brunswick

36

Bjurman Natalie Mount Allison University The risk of Borrelia infection (Lyme disease)in New Brunswick - using dogs as a sentinelspecies to assess the risk to humans"

37

Comeau Dominique Université de Moncton Characterization of Novel miRNAs Involved inBreast Cancer Metastasis

38

Patterson Jamie Mount Allison University Assessing the potential of Ixodes cookei as avector for Lyme Disease

39

Crapoulet Nicolas Atlantic CancerResearch Institute

Validation of two next-generation sequencingcancer panels for clinical testing

40

Boudreau Corinne Mount Allison University Experiences with chronic undiagnosed illnessin Canada

41

McGibbon Chris University of NewBrunswick

Instrumented pendulum test for objectivespasticity assessment of the knee

42

Hamilton-Wright Andrew Mount Allison University Determining the utility of mobile applicationsfor managing blood-iron in hemochromatosistreatment

43

Kemp Michael Vitalité Health Network A Naturalistic Study of Paliperidone Palmitatein Patients with Schizophrenia: Methods ofthe Canadian Sustenna TreatmentAssessment Registry (CanSTAR)

44

Desnoyers Guillaume Atlantic CancerResearch Institute

Decreased eIF3e expression can mediateepithelial-to-mesenchymal transition throughactivation of the TGF β signaling pathway

45

TRIVEDI PURVI Dalhousie MedicineNew Brunswick

Diet induced obesity and type 2 diabetesimpairs lysosomal autophagy in the murinemyocardium

46

Wang Hao Government of NewBrunswick

Trends in incidence and prevalence of heartfailure in New Brunswick

47

Belkaid Anissa Université de Moncton 17β-estradiol-induced breast cancer cell proliferation requires stearoyl-CoA desaturase-1 expression and activity

48

Robichaud Sarah Université de Moncton Contribution of the glutamine/glutamate cyclein response to STF-62247 revealed bymetabolic profiling : Correlation between thePI3K/AKT/FOXO pathway and autophagy

49

Lewis Julie Mount Allison University Assessing the risk of Lyme borreliosis in NewBrunswick

50

Bullerwell Charles E. Atlantic CancerResearch Institute

Characterization of the Pax5 interactome:PARP-1 can influence B-cell-specific geneexpression

51

Duguay Sabrina Université de Moncton SCD-1 activation in prostate cancer cells byandrogens

52


Paulin Anne Université de Moncton L'entrainement par exercice supramaximalrépété améliore la contribution lipidique à lafourniture énergétique lors d'un testincrémental chez des adultes obèses

53

Handrigan Grant Université de Moncton Childhood obesity affects balance control andaiming performance

54

Eadie Ashley Dalhousie MedicineNew Brunswick

The Role of Heme Regulatory Enzymes inMyocytes under Stress

55

Wall Michael Atlantic CancerResearch Institute

HNRNP H IS A NOVEL STRESS GRANULE-ASSOCIATED PROTEIN AND ASSISTS INCELLULAR STRESS RECOVERY

56

Arsenault Bishop Nicole Université de Moncton Dietary intake of DHA and EPA in a group ofpregnant women in the Moncton area

57

Jean Jonathan Stan Cassidy Centre forRehabilitation

Amyotrophic Lateral Sclerosis Incidence andCluster Identification in New Brunswick,Canada, over a 10-Year Period

58

Slade Logan University of NewBrunswick, Saint John

Dieldrin augments mTOR signaling andinhibits autophagy in the adult zebrafish heart(Danio rerio)

59

McCullum Shane Stan Cassidy Centre forRehabilitation

A One Year Pilot Study of Circuit ClassOutcomes in Outpatient NeurologicalPopulation

60

Cloutier Frank CHU-DUMONT A new surgical approach that usepolypropylene tapes for gynecologicalprolapse repair

61

Daigle Mikella Université de Moncton Expressions of Sox5 and Sox13 transcriptionfactors are increased in testicular Leydig cellsof mice during postnatal development/ Lesexpressions des facteurs de transcriptionSox5 et Sox13 augmentent dans les cellulesde Leydig du testicule murin lors dudéveloppement postnatal

62

Deprez Pierre Institut Atlantique deRecherche sur leCancer

Synthetic Lethality Screen of TumorSuppressor Pathways for TherapeuticCompound Development

63

Allain Eric Université de Moncton Investigation of the mechanisms by whichalternatively-spliced 5-lipoxygenase isoformsinhibit leukotriene biosynthesis

64

Roy Patrick Université de Moncton -Campus de Moncton

Effet de l'antagonisme des récepteursnicotiniques sur les monocytes

65

MacKenzie Jason U de Sherbrooke Ressemblance entre les pratiques de diverstypes d’activité physique chez des enfants en5e et 6e année et leurs parents

66

Melville Sarah Dalhousie MedicineNew Brunswick

Assessment of the Non-Invasive PulsewaveHealth Monitor^TM for Blood PressureDetermination in a Clinical Setting ofCardiovascular Catheterization/ Évaluation del’appareil non-invasive Pulsewave HealthMonitor^TM pour la détermination de lapression artérielle dans une clinique decathétérisation cardiaque

67


Philip Shona Dalhousie MedicineNew Brunswick

Nanoparticle delivery of curcumin to humanendothelial cells demonstrates efficientuptake and no overt toxicity to signficantlyinduce the expression of heme-oxygenase-1

68

Ward Stephanie Université deSherbrooke

Influence of childcare educators on physicalactivity and eating behaviours of preschool-aged children: A systematic review

69

Pulinilkunnil Thomas Dalhousie MedicineNew Brunswick

Angiotensin II acutely increases lysosomalautophagy in the murine myocardium

70

Graves Monica Dalhousie University A work in progress: Perceived barriers andenablers held by interprofessional teammembers around collaborating whencommunicating through the use of informationand communication technologies

71

Cote Claudia Dalhousie MedicineNew Brunswick

Effects of Transcatheter Aortic ValveImplantation on Cognitive Function

72

Title of the research project - Titre du projet de rechercheFunctional characterization of Mammaglobin-1 in breast cancer aggressiveness

Poster presenter - Nom de la personne qui présentera l'afficheRoxann Guerrette

First author - Premier auteurOui

Niveau de formationÉtudiants à la maîtrise

Researchers involved in this project - Chercheurs participant au projetGuerrette, Roxann; Picot, Nadia; Beauregard, Annie-Pier; Robichaud, Gilles.

Abstract - RésuméMetastasis is the major cause of death in women suffering from breast cancer. To provide a betterunderstand breast cancer progression, we have studied the role of mammaglobine-1 (MGB1) genein breast cancer pathogenesis. MGB1 has been extensively studied as a diagnostic biomarker dueto its abundant expression in mammary cancer cells. Yet, MGB1’s role in disease progression is stillunknown. Our experimental results demonstrate for the first time that MGB1 in a pivotal regulator onbreast cancer malignancy. More precisely, loss of MGB1 expression correlates with a decrease inproliferation, spheroid formation, migration, and invasion capacities of breast cancer cells.Concomitantly, we also observe that MGB1 expression activates pro-malignant signaling cascadessuch as MAPKs, focal adhesion kinase (FAK) and NFB pathways. Moreover, MGB1 promoteepithelial to mesenchymal (EMT) features which coincide with our findings. Our study provides thefirst evidence for MGB1 as regulator of breast cancer malignancy and disease progression.

Les métastases sont la cause majeure du décès relié au cancer du sein chez les femmes. Pourmieux comprendre la progression du cancer du sein, nous avons étudié le rôle du gènemammaglobine-1 (MGB1) dans la pathogénèse du cancer du sein. À cause de sa spécificité auxtissues mammaire et surexprimé dans la majorité des cancers du sein, MGB1 a été exhaustivementétudié pour ses caractéristiques d’un bon biomarqueur. Par contre, le rôle de MGB1 chez le cancerdu sein est encore inconnu. Nos résultats préliminaires montrent pour la première fois que MGB1est un régulateur important de la malignité du cancer du sein. Plus précisément, l’inhibition del’expression du gène MGB1 corrèle avec la prolifération, la formation de sphéroïdes, la migration etl’invasion des cellules du cancer du sein. Nous avons aussi observé que MGB1 favorisel’expression des gènes impliqué dans la malignité cellulaire dont la cascade des MAPKs, deskinases d’adhésion focale (FAK) et de NFB. De plus, MGB1 promeut la transition épithéliale àmesenchymal (EMT) ce qui coïncide avec nos résultats. Notre étude observe pour la première foisle rôle primordiale de MGB1 dans la progression et la malignité du cancer du sein.

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Title of the research project - Titre du projet de rechercheSupporting Men Engaging in Positive Healthy Practices in New-Brunswick:

Poster presenter - Nom de la personne qui présentera l'afficheJalila Jbilou

First author - Premier auteurNon spécifié

Niveau de formationAucune sélection

Researchers involved in this project - Chercheurs participant au projetPakzad, Sarah; El Adlouni, Salaheddine.

Abstract - RésuméRationale: Statistics show that the most prevalent men-related chronic health conditions, such asobesity, diabetes hypertension and cancer, have been on the rise over the last two decades inNew-Brunswick. What is troublesome is that these conditions are influenced by a small set ofwell-established, modifiable causes such as an unhealthy diet, and lack of physical activity.Community-based health education interventions seem to have better impact on increasing men’shealth literacy and motivation for healthy lifestyle. Objectives: 1) to assess the acceptability andfeasibility of a community-based intervention aiming to promote healthy practices among men; and2) to explore men’s needs and expectations in terms of healthy lifestyle educational interventionsand 3) to develop recommendations to support decision making among health professionals andmanagers Methods: We use the Intervention Mapping framework for planning health educationprograms. As a first phase, we conducted a qualitative study using semi-structured interviews withhealth professionals and focus groups composed exclusively of overweight/obese men. Verbatimwere analysed using a thematic content analysis. Results/Conclusion: Fifteen interviews and threefocus groups were done. The analysis revealed six major dimensions: 1)Adaptation of contentand format of the information 2)Delivery of health services in the milieux where men socialize3)Implementation of men sensitive health communication practices 4)Engagement of familymembers and significant others 5)Raising awareness and emphasis on the impacts of hegemonicmasculinity 6)Foster continuing education and training on men’s health among healthprofessionals and managers

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Title of the research project - Titre du projet de rechercheMiRNAs are important regulators of Pax-5 associated breast cancer processes

Poster presenter - Nom de la personne qui présentera l'afficheJason Harquail

First author - Premier auteurYes

Education LevelPhD Students

Researchers involved in this project - Chercheurs participant au projetHarquail, Jason; Robichaud, Gilles.

Abstract - RésuméRecent studies have enabled the identification of important factors regulating cancer progression,one of these being the Pax-5 gene. Pax-5, an essential developmental factor of B cells, is aberrantlyexpressed in various B cell cancer lesions and solid tumors such as breast carcinoma. AlthoughPax-5 downstream activity is relatively well characterized, the regulation of aberrant Pax-5expression in a cancer specific context is poorly understood. To investigate the regulation of Pax-5expression, we turned our attention to micro-RNAs (miRNAs), small non-coding RNA molecules thatregulate key biological processes. Extensive studies have shown miRNA deregulation in multiplecancer lesions. In this study, we aim to elucidate a causal link between differentially expressedmiRNAs in cancer cells and their putative targeting of Pax-5-dependent cancer processes. Withbioinformatics analyses, we observe that miRNAs 484 and 210 are aberrantly expressed in breastcancer cells and cross-reference with their predicted capacity to target the Pax-5 mRNA 3’untranslated region (3’UTR). Using anti- or pre-miRNAs transfected into Pax-5 expressing breastcancer cell lines, we demonstrate that miRNAs 484 and 210 are capable of regulating Pax-5expression. In addition, miRNA-dependent suppression of Pax-5 resulted in a concomitant alterationin Pax-5-mediated phenotype and cancer processes. This is the first study demonstrating theregulation of Pax-5 expression and function by non-coding RNAs in cancer cells. These findings willhelp us better understand aberrant Pax-5 expression levels within cancerous states creating thepossibility for earlier diagnosis as well as more targeted treatments for cancer patients.

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Title of the research project - Titre du projet de rechercheMind the Heart: Best Practices for Prevention, Early Identification and Treatment of Mood andAnxiety Disorders in Men with Heart Disease

Poster presenter - Nom de la personne qui présentera l'afficheNathalie N. Brodeur


Niveau de formationÉtudiants au doctorat

Researchers involved in this project - Chercheurs participant au projetBrodeur, Nathalie; Jbilou, Jalila; Chomienne, Marie-Hélène; Greenman, Paul; Grenier, Jean.

Abstract - RésuméAlmost twice as many men as women in Canada are hospitalized for ischemic heart diseases in agiven year. Recent research has revealed important relationships between HD and mood disorders(MD), anxiety disorders (AD), and trauma-related stress (TRS) in men. We propose to test andassess an integrated evidence-based intervention derived from UK’s stepped-care model for thetreatment of depression and anxiety in men following an Acute Coronary Syndrome (ACS).Objectives: In men with post ACS, we aim to (1) identify best practices that facilitate early detection,management, treatment and reduction of MD/AD/TRS; (2) assess needs, feasibility, andacceptability for early detection and stepped-care treatment of MD/AD/TRS; (3) assess theintervention and educational tools delivered in different settings and sites; (4) identify drivers andchallenges of implementation of the stepped-care model at the organizational, professional andpatient/family levels. Methods: We propose a multi-site project using a mixed method (qualitative;quantitative). We will validate, adapt and experiment a stepped-care intervention consisting ofeducational intervention in multiple settings, motivational interviewing and individual/group guidedself-help, face-to-face motivational interviewing and Problem Solving Treatment for individuals orcouples, and pharmacotherapy and/or specialized mental health care. Major outcomes:Improvement of access to effective evidence-based interventions for MD\AD\TRS in men with postACS, reduction of the double burden of disease in men with HD and strengthening the healthcaresystem’s capacity to respond to men’s mental health needs. Finally, we will adopt an integratedknowledge translation strategy for replication in other Canadian contexts to foster effectivedissemination.

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Title of the research project - Titre du projet de rechercheThe Development of an Isokinetic Adapter for Prosthesis Users

Poster presenter - Nom de la personne qui présentera l'afficheUsha Kuruganti

First author - Premier auteurNot specified

Education LevelNone selected

Researchers involved in this project - Chercheurs participant au projetKuruganti, Usha; Hamza, Sarah; Chester, Victoria.

Abstract - RésuméUpper limb amputations can have a significant impact on daily functions and affect the quality of lifeof an individual. There have been significant advances in the materials used to build these devicesresulting in lighter and stronger artificial limbs; however, users have indicated that improved functionand control strategies are desirable to become more in line with able-bodied limb function.Quantitative clinical assessment has been challenging due to the complexity of the musclephysiology of those with amputations. In addition, most clinical research has focused on studyingisometric (stationary) limb movements. In order to develop more robust systems, it is critical to studymuscle mechanics of those with amputations under dynamic (moving) movements. One method ofsafely examining dynamic movements is the use of isokinetic dynamometers. These machines allowmeasurement of upper and lower extremity isokinetic movements at controlled angular velocitieswhile ensuring no stress is placed on the individual (even if the participant is unable to move thelever arm). For able-bodied participants, this doesn’t present a problem. However, there is currentlyno commercially available isokinetic dynamometer adapter for prosthesis users. The purpose of thisproject was to develop an adapter that can be used by those with amputations to safely andeffectively operate the dynamometer. The tool that was developed connects to the arm of thedynamometer and is adjustable for different prosthesis users. The next step of this research is totest sample users to determine the tool’s reliability and validity.

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Title of the research project - Titre du projet de rechercheHusbands Living with Women on Dialysis: Embracing Their Transformed Life

Poster presenter - Nom de la personne qui présentera l'afficheMaryse Pelletier-Hibbert



Researchers involved in this project - Chercheurs participant au projet.

Abstract - RésuméThe purpose of this qualitative study was to explore the adjustment process of husbands living withwomen on dialysis. Using Glaser’s (1978) grounded theory methodology, data were collected from18 husbands from NB, NS, and PEI through semi-structured face-to-face or telephonetape-recorded interviews. The findings revealed that the most central issue for these husbands wasdealing with multiple changes in daily routines and future plans. While changes created manyhardships for husbands, witnessing their wives’ suffering was more distressing. In response, thesemen engaged in the basic social process of embracing their transformed life. The four stages ofembracing a transformed life are becoming aware, involving themselves, centering life on theirwives, and striving to achieve balance. The marital relationship, the women’s health status, as wellas the presence of informal support and formal support are conditions which significantly influencedthe process. The theory of embracing a transformed life provides a framework for understanding andexplaining the complex interplay of strategies undertaken by these husbands to respond to, adjustto, and integrate changes. Moreover, the focus on husbands living with women on dialysiscontributes towards closing an existing gap in knowledge and the findings underscore the husbands’abilities to learn and carry out complex roles, responsibilities, and routines that require sophisticatedobservation, decision making, and technical and problem-solving skills. The discrete stages of thetheory guide health care professional to better understand the various changes dialysis-caregiversmay experience during different phases of the patients’ illness trajectory and to implementsupportive care to enhance their adjustment and sustain their efforts.

Le but de cette étude qualitative était d’examiner le processus d’adaptation des hommes habitantavec une femme qui subit des traitements de dialyse. Les données ont été recueillies au coursd’entretiens semi-structurés enregistrés, réalisés en personne ou au téléphone, auprès de 18hommes mariés, conformément à la méthode de la théorie ancrée décrite par Glaser en 1978. Lesrésultats on démontré que la préoccupation centrale de ces hommes était de composer avec lesmultiples changements dans leurs activités quotidiennes et les projets futurs. Bien que ceschangements aient occasionné maintes difficultés pour ces hommes, il leur était encore plus difficilede voir leur femme souffrir. Ils se sont donc appliqués à leur apporter du soutien et à entamer leprocessus social fondamental qui consiste à accepter volontiers la transformation qui s’effectuedans leur vie. Les quatre étapes à suivre pour accepter la transformation d’une vie sont la prise deconscience, la participation, la centralisation de la vie sur la conjointe, et la recherche d’équilibre. Larelation de couple, la santé de la femme et la présence d’un soutien formel et informel sont tous desfacteurs qui ont une grande incidence sur le processus. La théorie qui consiste à accepter la

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transformation d’une vie propose un cadre qui permet de comprendre et d’expliquer l’ensemblecomplexe de stratégies qu’emploient ces hommes pour répondre aux changements, s’y adapter etles intégrer à leurs activités quotidiennes et à leur avenir. En outre, le fait de diriger le regard sur leshommes habitant avec une femme dialysée contribue à combler une lacune existante sur le plandes connaissances, et les constatations soulignent la capacité de ces hommes à apprendre et àmettre en pratique des rôles, des responsabilités et des routines complexes qui demandent descompétences avancées en observation, en prise de décision et en résolution de problème de mêmeque sur le plan technique. Les étapes subtiles de la théorie guident les infirmières vers unemeilleure compréhension des différents changements que les fournisseurs de soins de dialysepeuvent observer durant les différentes phases de la maladie de la patiente et leur permettentd’adopter des méthodes de soins de soutien qui facilitent leur adaptation et les poussent àpoursuivre leurs efforts.

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Title of the research project - Titre du projet de recherchePax-5 regulates breast cancer malignancy through the disruption of FAK1 signaling

Poster presenter - Nom de la personne qui présentera l'afficheSami Benzina


Niveau de formationBoursiers postdocoraux

Researchers involved in this project - Chercheurs participant au projetSami, Benzina; O'Brien, Pierre; Guérrette, Roxann; Beauregard, Annie-Pier; Jean, Stéphanie;Gilles, Robichaud.

Abstract - RésuméMetastasis accounts for 90% of deaths in breast cancers patients. Therefore, the study of geneticfactors regulating cancer malignancy is a top priority to mitigate the morbidity and mortalityassociated to this disease. One of these factors, Pax-5, normally regulates key biological functionssuch as cell viability, growth, and differentiation. However, an aberrant expression of this factorresults in the development and progression of cancer. In this study, we developed breast cancer cellmodels with conditioned expression of the Pax-5 to evaluate signaling pathways relevant to breastmetastasis and cancer progression. We found that Pax-5 extinguishes several aspects of canceraggressivety such as: proliferation, spheroid formation, migration and invasion. At the molecularlevel, we found that Pax-5 modulates cancer malignancy through the regulation of variouscomponents of the epithelial to mesenchymal transitioning (EMT) process in addition to keysignaling targets such as: NFB and the Focal Adhesion Kinase 1 (FAK1). We also demonstratethat Pax-5 decreases FAK1 level trough up-regulation of miR-135b, a direct repressor of FAK1expression. Altogether, our findings suggest that the presence of the Pax-5 lead to less aggressivebreast cancers by promoting mesenchymal to Epithelial transitioning (MET). These findings bringlight to molecular mechanisms driving breast cancer malignancy and benefit our quest in thedevelopment of diagnostic and therapeutic strategies against breast cancer progression.

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Title of the research project - Titre du projet de recherche3'UTR polymorphism and miRNA regulation of PAX5 expression in cancer cells

Poster presenter - Nom de la personne qui présentera l'afficheAnnie-Pier Beauregard


Education LevelMaster's Students

Researchers involved in this project - Chercheurs participant au projetGilles, Robichaud.

Abstract - RésuméRecent advances in biomedical research have allowed the identification of multiple genesresponsible in cancer development and progression. The PAX5 gene is pinpointed among thesemolecules that influence cellular behaviour in cancer. Although PAX5 plays an important role innormal cell development, its aberrant expression is involved in lymphoid cancer lesions and solidcancer progression. Although the downstream roles of PAX5 are relatively well described, fewstudies have focused on the regulation of PAX5 expression in cancer cells. Our team has thusturned its attention to possible miRNA regulation of PAX5 expression. MiRNAs are small moleculesthat regulate mRNA translation by targeting their 3’ untranslated region (3’UTR). Recent evidencehas demonstrated that many oncogenes evade miRNA regulation by expressing shorter 3’UTR. Ourgoal is to study and characterise PAX5 3’UTR editing and polymorphism. Upon our analysis of thePAX5 transcripts in breast and lymphocyte B cancer cells, we found that the PAX5 3’UTR region issubmitted to the alternative use of polyadenylation signals which result in premature truncation ofthe 3’UTR. We also identified alternative splicing within the 3’UTR in B cell cancers resulting in thedeletion of miRNA targeting sequences at the 3’end. Our characterisation of PAX5 3’UTR editing willreveal miRNA targeting and may elucidate PAX5 aberrant expression in cancer development andprogression. Further functional studies of PAX5 3’UTR polymorphism and its repercussions inmiRNA regulation will deepen our knowledge in PAX5-mediated cancer processes and will aid in thediscovery of new therapeutic or diagnostic strategies for cancer patients.

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Title of the research project - Titre du projet de rechercheCan microarray decipher the antimycobacterial mode of action of C17 diynes?

Poster presenter - Nom de la personne qui présentera l'afficheHaoxin Li



Researchers involved in this project - Chercheurs participant au projetAndrew, Cowie; Johnson, John; Webster, Duncan; Christopher, Martyniuk; Christopher , Gray.

Abstract - RésuméTreatment of microbial infections has become increasingly challenging due to increasing resistanceto current antibiotics and the lack of new drugs in the pipeline. One of the options to combat theseproblems is to identify molecules with novel modes of action for drug development. Theantimycobacterial activity of C17 diynes has been described previously, however, their mode ofaction remains unknown. Microarray techniques were used to explore the genetic regulationresponses of Mycobacterium smegmatis to treatment with the C17 diynes, falcarinol and panaxydol.Our analyses identified 704 and 907 differentially expressed genes respectively from falcarinol andpanaxydol treatments. Principal component analysis suggested a distinct mode of action of the C17diynes when compared with commonly used antimycobacterial drugs. In addition, functionalenrichment analysis and pathway enrichment analysis revealed statistically significant gene ontology(GO) terms and pathways from falcarinol and panaxydol treatments respectively. The potentialbioactivities of the two C17 diynes were predicted through prediction of activity spectra ofsubstances (PASS). Combining the results of all three analyses, we can hypothesize that the C17diynes inhibit fatty acid biosynthesis, specifically phospholipid synthesis, in mycobacteria. Althoughwe did not identify the specific protein binding site of C17 diynes, we were able to develop a specifichypothesis for the mode of action of C17 diynes that can now be tested through focussed additionalexperiments and unambiguously reveal the antimycobacterial mode of action of C17 diynes.

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Title of the research project - Titre du projet de rechercheMetabolomic approaches to increasing the efficiency of natural products for drug discovery

Poster presenter - Nom de la personne qui présentera l'afficheTrevor N. Clark



Researchers involved in this project - Chercheurs participant au projetClark, Trevor; Johnson, John; Gray, Christopher.

Abstract - RésuméNatural products continue to play an unparalleled role in drug discovery and development. Fungalendophytes are a relatively unstudied source of new bioactive natural products and are a potentialsolution to this problem. Typically we use a suite of bioassays to test fungal extracts against variouspathogenic organisms or cell lines that direct us in the isolation of bioactive compounds; howeverthis often leads to the isolation of known chemicals which is undesirable when looking for new drugcandidates. Metabolomics has emerged in the field of drug discovery as a way to prioritize extractsbased on their chemical composition rather than on bioassay data alone. These methods havebeen applied to a library of 81 fungal endophytes isolated from the leaves of traditionally usedmedicinal plants. Nuclear Magnetic Resonance (NMR) based metabolomics analysis led to theisolation of three bioactive compounds from this library. Because of the low sensitivity inherent inNMR analyses, all three compounds were known but our investigations revealed previouslyunreported bioactivities for each and clearly demonstrated the potential of the combinedmetabolomics-bioactivity approach to project prioritization. As a result, we are now exploring the useof high resolution mass spectrometry (HRMS) based methods with high analytical sensitivity thatmay be a much more powerful metabolomics tool. The use of HRMS based metabolomicscombined with our bioactivity screening should allow for greatly increased efficiency in ourprioritization of extracts and focus our research efforts towards the discovery of novel bioactivechemistry.

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Title of the research project - Titre du projet de recherchePowered Wrist Motion - Independent Wrist Motion for Transradial Myoelectric Prostheses

Poster presenter - Nom de la personne qui présentera l'afficheAli Hussaini



Researchers involved in this project - Chercheurs participant au projetHussaini, Ali; Kyberd, Peter.

Abstract - RésuméCurrent transradial prostheses which incorporate a wrist rotation unit do not allow for simultaneouscontrol of wrist rotation and terminal device. Independent control is achieved by co-contraction (i.e.mode switching), physical switches (toggle, pushbutton) or specialized cable-harness mechanisms.This research investigated simultaneous control of the wrist and hand function by reengineering theprosthetic socket interface and augmenting the available control input from the user. The primaryfocus was to determine if a viable control input could be acquired from the user’s forearm rotation(e.g. pronation, supination) alongside surface EMG signals that drive hand function. These inputswould then be used to control two degrees of freedom simultaneously. A novel design for atransradial prosthesis was developed that allows for independent control of a wrist rotation unit anda single degree of freedom prosthetic hand. The functionality of this prosthesis was evaluated by aprosthesis user who performed the Box and Blocks test and a clothespin relocation task. Outcomemeasures included time to complete a task, number of blocks moved, and analysis of kinematicangles using a motion capture system. Comparisons were made between the new prosthesis, theuser’s current prosthesis, and a benchmark group of 13 able-bodied subjects. Results indicated adecrease in compensatory motions of the trunk, head, and shoulder angles in certain activities, butan increase in time to complete the task with the new design. Feedback from the prosthesis userfavoured the functionality provided by the wrist rotator and associated control strategy.

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Title of the research project - Titre du projet de rechercheMedication adherence in university students

Poster presenter - Nom de la personne qui présentera l'afficheOdette Gould



Researchers involved in this project - Chercheurs participant au projetHamilton, Hillary; Doucette, Clara.

Abstract - RésuméStudies in Ontario suggest that up to one in 7 university students has been treated for mental healthproblems (ACHA-NCHA II student survey, 2009). For many, university years are the first timestudents are taking medication without parental supervision, yet little is known about students’medication adherence. The present study had two purposes: (a) to identify predictors of students’non-adherence behaviours, and (b) to describe students’ attitudes surrounding medication fordepression, anxiety, and ADD. First, 70 undergraduate students receiving medication to treatdepression, anxiety or ADD/ADHD filled out an on-line survey where they provided a self-report ofmedication adherence, and rated a series of factors on the extent to which they affected adherence.Second, four students taking prescription medication to treat depression, anxiety or ADD/ADHDparticipated in semi-structured interviews where they discussed their experiences and attitudessurrounding their condition and its treatment. Thirty percent of students reported not taking theirmedications as prescribed in the past month. Significant predictors of non-adherence includedinaccurate beliefs of medication, and short-term physical and psychological side effects. Ininterviews, students reported obtaining medication from physicians with few questions and no delay,and follow-up was minimal or absent. The fact that very few students are receiving optimal care (i.e.,combined psychotherapy, lifestyle change recommendation and medication) is a concern.

L’ADHERENCE AUX MEDICAMENTS CHEZ LES ETUDIANTS UNIVERSITAIRES La rechercheauprès des étudiants universitaires en Ontario suggère que presque un étudiant sur 7 a reçu destraitements pour des problèmes de santé mental (ACHA-NCHA II sondage étudiant, 2009). Pourplusieurs, la transition à l’université offre la première opportunité pour prendre ses médicamentssans la supervision des parents, mais peu de recherche a étudié l’adhérence aux médicamentschez les étudiants. Ce projet avait deux buts: (a) d’identifier les variables associées à lanon-adhérence aux médicaments pour la dépression, l’anxiété et TDA/TDAH, et (b) d’explorer lesattitudes des étudiants envers ces médicaments. D’abord, 70 étudiants ayant reçu une prescriptionont remplis un questionnaire en ligne ou ils ont indiqué leur niveau d’adhérence, et ont évalué unesérie de variables pouvant affecter l’adhérence. Ensuite, quatre étudiants ont participé à unentrevue pour discuter de leurs expériences autour de l’adhérence et de l’obtention demédicaments. Trente pourcent des étudiants ont rapporté n’avoir pas pris leur médicaments tel queprescrit pendant le mois précédent. Les croyances vis-à-vis les médicaments, et les effetssecondaires physiques et psychologiques avaient des corrélations significatives et négatives avecl’adhérence. Dans les entrevues, les étudiants ont rapporté avoir obtenu des médicamentsrapidement et aisément, et le suivi était minimal. Nos résultats sont inquiétants du point de vue que

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peu d’étudiants reçoivent les soins optimaux, c’est-à-dire une combinaison de médicaments, depsychothérapie, et de modifications de style de vie.

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Title of the research project - Titre du projet de rechercheMedication adherence in university students

Poster presenter - Nom de la personne qui présentera l'afficheOdette Gould



Researchers involved in this project - Chercheurs participant au projetHamilton, Hillary; Doucette, Clara.

Abstract - RésuméStudies in Ontario suggest that up to one in 7 university students has been treated for mental healthproblems (ACHA-NCHA II student survey, 2009). For many, university years are the first timestudents are taking medication without parental supervision, yet little is known about students’medication adherence. The present study had two purposes: (a) to identify predictors of students’non-adherence behaviours, and (b) to describe students’ attitudes surrounding medication fordepression, anxiety, and ADD. First, 70 undergraduate students receiving medication to treatdepression, anxiety or ADD/ADHD filled out an on-line survey where they provided a self-report ofmedication adherence, and rated a series of factors on the extent to which they affected adherence.Second, four students taking prescription medication to treat depression, anxiety or ADD/ADHDparticipated in semi-structured interviews where they discussed their experiences and attitudessurrounding their condition and its treatment. Thirty percent of students reported not taking theirmedications as prescribed in the past month. Significant predictors of non-adherence includedinaccurate beliefs of medication, and short-term physical and psychological side effects. Ininterviews, students reported obtaining medication from physicians with few questions and no delay,and follow-up was minimal or absent. The fact that very few students are receiving optimal care (i.e.,combined psychotherapy, lifestyle change recommendation and medication) is a concern.

L’ADHERENCE AUX MEDICAMENTS CHEZ LES ETUDIANTS UNIVERSITAIRES La rechercheauprès des étudiants universitaires en Ontario suggère que presque un étudiant sur 7 a reçu destraitements pour des problèmes de santé mental (ACHA-NCHA II sondage étudiant, 2009). Pourplusieurs, la transition à l’université offre la première opportunité pour prendre ses médicamentssans la supervision des parents, mais peu de recherche a étudié l’adhérence aux médicamentschez les étudiants. Ce projet avait deux buts: (a) d’identifier les variables associées à lanon-adhérence aux médicaments pour la dépression, l’anxiété et TDA/TDAH, et (b) d’explorer lesattitudes des étudiants envers ces médicaments. D’abord, 70 étudiants ayant reçu une prescriptionont remplis un questionnaire en ligne ou ils ont indiqué leur niveau d’adhérence, et ont évalué unesérie de variables pouvant affecter l’adhérence. Ensuite, quatre étudiants ont participé à unentrevue pour discuter de leurs expériences autour de l’adhérence et de l’obtention demédicaments. Trente pourcent des étudiants ont rapporté n’avoir pas pris leur médicaments tel queprescrit pendant le mois précédent. Les croyances vis-à-vis les médicaments, et les effetssecondaires physiques et psychologiques avaient des corrélations significatives et négatives avecl’adhérence. Dans les entrevues, les étudiants ont rapporté avoir obtenu des médicamentsrapidement et aisément, et le suivi était minimal. Nos résultats sont inquiétants du point de vue que

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Title of the research project - Titre du projet de rechercheMen’s health promotion and work engagement in the context of workplace bullying.

Poster presenter - Nom de la personne qui présentera l'afficheLaura Aubin, Donna Bulman, and Judith MacIntosh



Researchers involved in this project - Chercheurs participant au projetAubin, Laura.

Abstract - RésuméWorkplace bullying is repeated abuse that often results in feelings of personal diminishment withlong-term health consequences. The consequences may include a decrease in psychological,economic, social and physical well-being. The objective of our study was to explore whether or notsupport mediates the damaging effects of workplace bullying. This is a qualitative study that is partof a larger study on workplace bullying funded by CIHR, NBHRF, and UNB (MacIntosh, Wuest, &Bulman). We analyzed the data from 16 interviews with men who sought and received support afterexperiencing bullying in the workplace. Demographic information on these sixteen participantsincludes age, type of workplace, patterns of bullying, and type of employment. Grounded theorytechniques were used in order to analyze and interpret the data. Our findings help to debunk themyth that men who are bullied do not seek support. Men in our study sought support from a varietyof sources, including, co-workers, spouses, management, unions, friends, and health careprofessionals. We found that when support was effective men felt valued and heard. The supportalso helped them to manage the bullying and move on with their lives. Conversely, when supportwas ineffective or not offered men felt furthered abused, and abandoned, contributing to worsenedhealth.

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Title of the research project - Titre du projet de rechercheEffects of workplace bullying on men

Poster presenter - Nom de la personne qui présentera l'afficheJudith MacIntosh and Denise LeBlanc-Kwaw



Researchers involved in this project - Chercheurs participant au projetMacIntosh, Judith ; LeBlanc-Kwaw, Denise ; Bulman, Donna; Wuest, Judith.

Abstract - RésuméWorkplace bullying is repeated abuse consisting of offensive or unwanted behaviours that humiliateor intimidate another at work. Health effects include long-term social, physical, psychological, andeconomic impacts. We used grounded theory to understand men’s experiences of being bullied atwork. We developed a substantive theory of how bullied men took care of their health and engagedin the workforce by interviewing a community sample of 36 adult men. We have demographic dataon age, education, kind of work, nature of bullying, and kinds of workplaces. About 2/3 of men werebullied by bosses and about 2/3 of those bosses were men. An important finding was thatworkplace bullying eroded men’s sense of self and how they thought of themselves. Men addressedthis problem of erosion using a four stage process we named Sustaining Self. We called the stagesNoting Discrepancies, Gauging Damage, Preserving, and Coming to Terms. Of significance, too, isthat men’s efforts to take care of their health and to keep working were affected by the support theyreceived and how they thought they were expected to behave as men. We learned how menresponded to workplace bullying and how health professionals can assist them in managing theeffects. This study was funded by a CIHR Operating Grant, NBHRF RPP, and UNB.

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Title of the research project - Titre du projet de rechercheCan pictures avoid redundancy? The use of bioactivity profiling to prioritise endophyte naturalproduct isolations

Poster presenter - Nom de la personne qui présentera l'afficheAndrew J Flewelling



Researchers involved in this project - Chercheurs participant au projetFlewelling, Andrew; Johnson, John; Gray, Christopher.

Abstract - RésuméThe natural products chemistry of marine endophytic fungi is an area where relatively few studieshave been undertaken despite them being recognized as a promising source of new antimicrobialnatural products. One hundred and forty-two endophytic fungi have been isolated from 20 speciesof marine macroalgae from the Bay of Fundy, Canada. Crude extracts obtained from theseendophytes have been screened for their antimicrobial activity against Staphylococcus aureus,methicillin-resistant S. aureus, Pseudomonas aeruginosa, and Candida albicans. Forty-eightendophytes (34% of the library) were found to be bioactive (Kruskal-Wallis p < 0.01) against at leastone of the test pathogens. Traditionally, crude extracts that exhibit promising activity from initialscreening assays would be fractionated according to their degree of activity, a method thatfrequently leads to redundancy when isolating natural products. The use of bioactivity profilingpresents a unique approach for prioritising crude extracts for fractionation with the goal of identifyingnew antimicrobial natural products with novel modes of action or targets. Bioactivity profiles ofextracts are derived from data acquired against a suite of pathogenic microorganisms and can becompared to the profiles obtained for known antibiotics that represent a range of modes of action.Those crude extracts exhibiting unique profiles are then prioritised and fractionated. The use ofbioactivity profiling will facilitate a more focused choice when selecting crude extracts forfractionation, while reducing the isolation and identification of known natural products.

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Title of the research project - Titre du projet de rechercheCanadian medicinal plants… a source of anticancer drug leads?

Poster presenter - Nom de la personne qui présentera l'afficheAllyson Bos



Researchers involved in this project - Chercheurs participant au projetBos, Allyson; Jean, Stéphanie ; Robichaud, Gilles; Johnson, John ; Gray, Christopher.

Abstract - Résumé The development of new cancer drugs in the treatment of mammary tumours is imperative. Despitesignificant advances in screening technologies and treatments, breast cancer remains the mostcommonly diagnosed cancer and second leading cause of cancer deaths among Canadian women.One strategy in identifying potential anticancer drug candidates is through investigation of the effectof plant-derived natural products on apoptotic activity in tumour cells. Preliminary screening of ourlibrary of crude extracts from 35 traditionally used Canadian medicinal plants has identified 23extracts that exhibit potent anti-proliferative and apoptotic inducing activities against the highlymalignant breast carcinoma cell line, MDA-MB-231. Building on previous work in our group, we haveidentified the active constituents in extracts of Juniperus communis and Aralia nudicaulis, thusvalidating our experimental approach. We are currently isolating the chemical constituentsresponsible for the observed anticancer activities with the objective of finding novel chemicalscaffolding to serve as potential anticancer drug leads.

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Title of the research project - Titre du projet de rechercheVariation of the amount of falcarindiol, an antimycobacterial natural product, within the Canadianmedicinal plant Heracleum maximum

Poster presenter - Nom de la personne qui présentera l'afficheKathryn Melvin



Researchers involved in this project - Chercheurs participant au projetMelvin, Kathryn; O'Neill, Taryn E. ; Webster, Duncan; Houlahan, Jeff. E. ; Johnson, John A.; Gray,Christopher A. .

Abstract - RésuméThe root of Heracleum maximum has been traditionally used by the First Nations of the MaritimeProvinces as a tuberculosis therapy as well as for other medicinal purposes. Falcarindiol, a C17diyne was determined to be the primary active constituent of the plant. These data, in combinationwith falcarindiol’s reported health benefits, stimulated our interest in this compound. To facilitatefurther research and development of H. maximum extracts and falcarindiol, we required a reliablesource of the natural product. We therefore conducted an evaluation of the variability of theantimycobacterial activity and falcarindiol content of various H. maximum organs between seasons. Individual H. maximum plants were collected in spring, summer and fall seasons of 2010, 2011 and2012. Our data indicate that the antimycobacterial activity of H. maximum is highly variable betweenyears, seasons and plant organs. Antimycobacterial activity tended to be highest in the summer andextracts from flowers generally produced the most bioactivity. Similarly, we found that the amount offalcarindiol is also variable between years, seasons and plant organs, but there is a low correlationbetween falcarindiol content and extract antimycobacterial activity. Our data suggests thatfalcarindiol is not the sole antimycobacterial compound produced by H. maximum indicating theplant should be further investigated as a source of antimycobacterial natural products. These resultshave also provided important insights regarding where and when to harvest plant material as asource of falcarindiol if extracts of H. maximum are to be developed as natural health products in thefuture.

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Title of the research project - Titre du projet de rechercheEffect of a supramaximal exercise on FFA levels in obese adolescent boys

Poster presenter - Nom de la personne qui présentera l'afficheJeremy Cormier

First author - Premier auteurNon spécifié

Niveau de formationÉtudiants de 1er cycle

Researchers involved in this project - Chercheurs participant au projetCormier, Jeremy; Iancu, Horia-Daniel; Paulin, Anne.

Abstract - RésuméBackground: The fat utilization is largely modulated by plasma FFA concentrations and greater FFAconcentrations suggest that plasma FFA from hydrolysis of adipose tissue triglycerides canpotentially meet the oxidative needs of working muscle. Objective: The aim of this work was toexamine the effect of supramaximal exercise (SME) on the plasma free fatty acid (FFA)concentrations in sedentary adolescent boys with different body weight statuses. Both plasmacatecholamine and insulin levels which are largely involved in the lipolysis process were alsoevaluated. Methods: Normal-weight (NW), overweight (OW), and obese (OB) adolescent boys (n=15 per group) completed a SME bout consisting of 6 x 6s maximal sprints with 2 min of passiverest between each repetition. Plasma epinephrine (E), norepinephrine (NE), insulin and FFAconcentrations were determined at rest, immediately after a 10 min warm-up, immediately afterSME, and after 20 min of passive recovery. Results: The E and NE concentrations measuredimmediately after SME and following 20 min of recovery were significantly lower in the OB than inthe OW and NW (p<0.01). Moreover, the insulin concentration was significantly higher in the OBthan in the NW and OW (p<0.01 and p<0.01 respectively). At the same time, FFA concentrationswere higher in the OB than in the OW and NW (p<0.01 and p<0.01 respectively). Conclusion:Despite lower plasma catecholamine responses, the FFA concentrations increased significantly inOB compared to NW and OW in response to the SME. This may reflect an increased lipidmobilization to energy supply.

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Title of the research project - Titre du projet de rechercheEffect of Low Frequency Neuromuscular Electrical Stimulation on Glucose profile of Persons withType 2 Diabetes: A pilot study

Poster presenter - Nom de la personne qui présentera l'afficheGeorges Jabbour



Researchers involved in this project - Chercheurs participant au projetBeliveau, Iise; Probizanki, David; Newhouse, Ian; MacAuliffe, Jim; Jakobi, Jennifer; Johnson,Michel.

Abstract - RésuméBackground: Neuromuscular electrical stimulation (NMES) improves metabolic control in sedentaryT2D patients. The most common protocols used were NMES at 35 HZ and up. However, manypatients complained of pain, discomfort, and/or constraints during NMES sessions. Low-frequencyNMES elicits significant muscle contractions that are well tolerated in asymptomatic populations forextended periods of time. Objective: The purpose of this study was to examine the effect oflow-frequency neuromuscular electrical stimulation (NMES) on glucose profile in persons with type 2diabetes (T2D). Methods: Eight persons with T2D (41 to 65 years) completed a glucose tolerancetest with and without NMES delivered to the knee extensors for a 1-hour period at 8Hz. Three bloodsamples were collected: at rest, and then 60 and 120 minutes after consumption of a glucose loadon the NMES and control days. Results: In NMES groups glucose concentrations were significantlylower (p<0.01) than in the control conditions. Moreover, a significant positive correlation (r=0.9;p<0.01) was obtained between the intensity of stimulation and changes in blood glucose.Conclusion: While stimulation parameters used in previous studies were quite diverse andphysiological responses were difficult to compare, low-frequency NMES (8 Hz), which are toleratedby all experimental individual, induced enhance in glucose uptake in persons with T2D. However,evaluation of the chronic effect of low-frequency NMSE with particular attention on the interactionbetween stimulation intensity and muscle fatigue parameters, on glucose regulation and on othermetabolic, hormonal and muscle parameters in T2D patients remains an interesting future study.

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Title of the research project - Titre du projet de rechercheBarking up the right tree

Poster presenter - Nom de la personne qui présentera l'afficheKathleen Complak



Researchers involved in this project - Chercheurs participant au projetComplak, Kathleen; Gray, Christopher; Johnson, John.

Abstract - RésuméThe overall goal of our work is to discover new sources of natural products with human healthapplications. The First Nations peoples in Canada have used tree tissues as curatives for a varietyof ailments, with bark being often used specifically for cold and tuberculosis remedies. Endophytesoccurring asymptomatically within plant tissues are known to produce bioactive natural products,suggesting that they could be the underlying source of the tree bark’s medicinal properties. Fifteentrees that have been traditionally used as medicines were selected based upon their therapeuticuse, collected in New Brunswick and investigated for their barks’ potential to host endophytic fungiable to produce new bioactive compounds. Endophytes were isolated from the outer bark of thetrees before being fermented in liquid culture. Extracts of the fermented broths were screenedagainst two bacterial pathogens (Staphylococcus aureus and Pseudomonas aeruginosa) and onefungal pathogen (Candida albicans) to assess their antimicrobial activity. Results indicate thattwenty fungal extracts have significant inhibitory activity against Pseudomonas aeruginosa, and fourare significantly active against Candida albicans. We are currently screening these extracts forantimycobacterial activity and isolating the natural product constituents that are responsible for theobserved extract bioactivity.

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Title of the research project - Titre du projet de rechercheChange the recipe, change the dish

Poster presenter - Nom de la personne qui présentera l'afficheMorgan Sproul



Researchers involved in this project - Chercheurs participant au projetMorgan , Sproul; Jeff, Fournier ; John , Johnson; Christopher, Gray.

Abstract - RésuméFungal endophytes have numerous genes that encode for natural products but are not expressedunder standard laboratory conditions; the existence of silent, or cryptic, biosynthetic genes is welldocumented. Inducing expression of silent genes may elicit biosynthesis of novel natural productsand thus be a source for the next-generation of antibiotics. This study explored the effects ofculturing endophytes under a variety of conditions such as media composition and fermentationvessels as well as introducing stimuli during fermentation such as co-culture and chemical shock.Our results show that varying fermentation vessel influences biosynthesis of natural products;greater bioactivity against test pathogens was observed in crude extracts obtained from culturesgrown in Erlenmeyer flasks compared to those in scintillation vials. Altering media composition gavecrude extracts with different degrees of bioactivity; endophytes cultured in yeast extract brothproduced extracts with lower bioactivity than those obtained from endophytes grown in potatodextrose broth. Surprisingly, crude extracts obtained from endophytes grown as co-cultures andcultures that had been subjected to chemical shock did not have greater bioactivity than culturesgrown under standard laboratory conditions. This study shows that manipulating culture conditionscan greatly influence natural product biosynthesis in endophytic fungi, but these effects are highlyvariable and difficult to quantify at the level of extract bioactivity. Future work will include the use ofmetabolomic techniques to analyse changes in extract composition that will allow differences inextracts to be identified at the molecular level.

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Title of the research project - Titre du projet de rechercheIntroduction du dosage STAT de la procalcitonine au Centre hospitalier universitaireDr-Georges-L.-Dumont / Introduction of a STAT procalcitonin assay at the Dr. Georges-L.-DumontUniversity Hospital Centre

Poster presenter - Nom de la personne qui présentera l'afficheDr Gabriel Girouard



Researchers involved in this project - Chercheurs participant au projetGiroaurd, Gabriel; Bouhtiauy, Ihssan.

Abstract - RésuméLe sepsis constitue l’une des premières causes de décès en milieu hospitalier au monde. Un délaidans le diagnostic du sepsis et le début de traitement antimicrobien se traduit par une augmentationsignificative de la mortalité associée à cette infection systémique. La nécessité d’accéder à un testdiagnostique rapide est primordiale afin d’assurer une prise en charge thérapeutique immédiate etadéquate. La procalcitonine (PCT) se révèle de plus en plus comme étant le biomarqueur parexcellence de l’infection d’origine bactérienne. La concentration sanguine de PCT est quasiindétectable à l’état normal ou lors d’une infection virale (<0.1 ng/ml) contrairement à des niveauxde 0.25 ng/ml et plus lors d’une infection bactérienne. Le but de la présente étude est d’évaluerl’impact de l’introduction du dosage STAT de la PCT au CHU Dumont. Plus spécifiquement, cetteétude permettra : 1) de déterminer la sensibilité et la spécificité du dosage de la PCT; 2) d’évaluerl’impact de ce dosage sur la prise de décision et la pratique clinique; 3) de mesurer l’impact dudosage de la PCT sur l’utilisation des antibiotiques et les économies qui y sont associés. Desrésultats préliminaires obtenus de dosages préalables de la PCT sur 18 échantillons démontrentl’utilité du test dans le diagnostic d’infections variées d’origine bactérienne. Agrément Canada exigeque les hôpitaux possèdent un programme rigoureux de gérance des antimicrobiens. Le dosage dela PCT offre un outil majeur aux cliniciens pour le diagnostic rapide du sepsis, tout en leurpermettant de mieux prescrire les antibiotiques.

Sepsis is one of the leading causes of death in hospitalized patients around world. A delay in thediagnosis and treatment of sepsis is associated with increased mortality rates. The availability of arapid diagnostic test is essential to ensure the immediate treatment and management of systemicinfections. Many studies have demonstrated that procalcitonin (PCT) constitutes a biomarker ofchoice for the diagnosis of bacterial infections. Blood levels of PCT are almost undetectable inhealthy individuals or during viral infections (<0.1 ng/ml) versus levels of 0.25 ng/ml or more duringbacterial infections. The goal of this study is to evaluate the impact of the introduction of a STATPCT assay at CHU Dumont. Specifically, this study will: 1) determine the sensitivity and specificity ofthe assay; 2) assess the impact of this assay on decision making and clinical practice; 3) measurethe impact of this assay on antibiotic use and associated cost savings. We performed a priorevaluation of PCT on 18 samples. Preliminary results demonstrate its usefulness for the diagnosisof a variety of bacterial infections. Accreditation Canada requires that hospitals have in place arigorous antimicrobial stewardship program. The PCT assay constitutes an important tool for

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Title of the research project - Titre du projet de rechercheIntroduction du dosage STAT de la procalcitonine au Centre hospitalier universitaireDr-Georges-L.-Dumont / Introduction of a STAT procalcitonin assay at the Dr. Georges-L.-DumontUniversity Hospital Centre

Poster presenter - Nom de la personne qui présentera l'afficheDr Gabriel Girouard



Researchers involved in this project - Chercheurs participant au projetGiroaurd, Gabriel; Bouhtiauy, Ihssan.

Abstract - RésuméLe sepsis constitue l’une des premières causes de décès en milieu hospitalier au monde. Un délaidans le diagnostic du sepsis et le début de traitement antimicrobien se traduit par une augmentationsignificative de la mortalité associée à cette infection systémique. La nécessité d’accéder à un testdiagnostique rapide est primordiale afin d’assurer une prise en charge thérapeutique immédiate etadéquate. La procalcitonine (PCT) se révèle de plus en plus comme étant le biomarqueur parexcellence de l’infection d’origine bactérienne. La concentration sanguine de PCT est quasiindétectable à l’état normal ou lors d’une infection virale (<0.1 ng/ml) contrairement à des niveauxde 0.25 ng/ml et plus lors d’une infection bactérienne. Le but de la présente étude est d’évaluerl’impact de l’introduction du dosage STAT de la PCT au CHU Dumont. Plus spécifiquement, cetteétude permettra : 1) de déterminer la sensibilité et la spécificité du dosage de la PCT; 2) d’évaluerl’impact de ce dosage sur la prise de décision et la pratique clinique; 3) de mesurer l’impact dudosage de la PCT sur l’utilisation des antibiotiques et les économies qui y sont associés. Desrésultats préliminaires obtenus de dosages préalables de la PCT sur 18 échantillons démontrentl’utilité du test dans le diagnostic d’infections variées d’origine bactérienne. Agrément Canada exigeque les hôpitaux possèdent un programme rigoureux de gérance des antimicrobiens. Le dosage dela PCT offre un outil majeur aux cliniciens pour le diagnostic rapide du sepsis, tout en leurpermettant de mieux prescrire les antibiotiques.

Sepsis is one of the leading causes of death in hospitalized patients around world. A delay in thediagnosis and treatment of sepsis is associated with increased mortality rates. The availability of arapid diagnostic test is essential to ensure the immediate treatment and management of systemicinfections. Many studies have demonstrated that procalcitonin (PCT) constitutes a biomarker ofchoice for the diagnosis of bacterial infections. Blood levels of PCT are almost undetectable inhealthy individuals or during viral infections (<0.1 ng/ml) versus levels of 0.25 ng/ml or more duringbacterial infections. The goal of this study is to evaluate the impact of the introduction of a STATPCT assay at CHU Dumont. Specifically, this study will: 1) determine the sensitivity and specificity ofthe assay; 2) assess the impact of this assay on decision making and clinical practice; 3) measurethe impact of this assay on antibiotic use and associated cost savings. We performed a priorevaluation of PCT on 18 samples. Preliminary results demonstrate its usefulness for the diagnosisof a variety of bacterial infections. Accreditation Canada requires that hospitals have in place arigorous antimicrobial stewardship program. The PCT assay constitutes an important tool for

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clinicians, allowing them to rapidly diagnose sepsis all the while informing their antibiotic prescribingpractices.

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Title of the research project - Titre du projet de rechercheInhibition des lysosomes et modulation du métabolisme de la sphingosine pour cibler les cellulescancéreuses rénales

Poster presenter - Nom de la personne qui présentera l'afficheMaxime Cahuzac



Researchers involved in this project - Chercheurs participant au projetCahuzac, Maxime; Turcotte, Sandra.

Abstract - RésuméIntroduction. Le carcinome rénal à cellules claires (RCC) représente près de 80% des cas de cancerdu rein. Une inactivation du gène de suppression tumorale, von Hippel-Lindau (VHL), est rapportéedans la majorité des RCC. Le laboratoire a identifié une petite molécule, le STF-62247, pouvantinduire la mort sélective des cellules inactives en VHL sans affecter la viabilité des cellules dont VHLest fonctionnel. La toxicité de cette molécule est associée à une modulation de l’autophagie et à uneperméabilisation des membranes lysosomales (LMP). Objectifs. Notre recherche a pour but dedéterminer si cet évènement observé dans les cellules déficientes VHL est lié aux sphingolipides.Méthodes. Des analyses par qPCR ont permis de mesurer l’expression des gènes codant pourl’acide sphingomyélinase (SMPD1), la sphingomyélinase neutre (SMPD2-4) et l’acide céramidase(ASAH1). De plus, l’effet d’agents cationiques, d’inhibiteurs de la pompe V-ATPases et dessphingosines kinases (SPHK) sur la viabilité des cellules RCC a été évalué par XTT. Résultats. Nosrésultats démontrent que l’expression de SMPD1 et d’ASAH1 augmente de façon significative chezles cellules déficientes VHL en réponse au STF-62247. Les agents cationiques utilisés se sontavérés peu toxiques chez les RCC alors que les inhibiteurs de la pompe V-ATPase et de SPHK2ont démontré une spécificité pour les cellules déficientes VHL. Conclusion. Les données obtenuessuggèrent qu’une modulation de la sphingosine pourrait être impliquée dans la toxicité sélective auSTF-62247 des cellules RCC dont le gène VHL est inactif et que celles-ci pourraient être ciblées pardes inhibiteurs de lysosomes.

Lysosomal agents and modulation of sphingosine to target kidney cancer cells Background. Renalcell carcinomas (RCC) represent almost 80% of all kidney cancer cases. The von Hippel-Lindau(VHL) tumor suppressor gene is inactivated in the majority of RCC. The laboratory previouslyidentified a novel small molecule, STF-62247, which is specifically toxic in VHL-deficient cellswithout affecting the viability of cells where VHL is functional. The cytotoxicity of this small moleculeis associated with a modulation of autophagy and lysosomal membranes permeabilisation (LMP).Objective. In this study, we investigate if LMP observed in response to STF-62247 in VHL-deficientcells is linked to sphingolipids metabolism. Methods. Analyzes of qPCR have been used to measurethe expression of different genes coding for acid sphingomyelinase (SMPD1), neutralsphingomyelinase (SMPD2-4) and acid ceramidase (ASAH1). Moreover, RCC viability has beenevaluated in presence of cationic agents, V-ATPase inhibitors and sphingosine kinase (SPHK)inhibitors by XTT test. Results. Our results demonstrate that the expression of SMPD1 and ASAH1increase significantly in VHL-deficient cells in response to STF-62247. The cationic agents are

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slightly toxic in RCC while the inhibitors of V-ATPase and SPHK2 demonstrated specificity forVHL-deficient cells. Conclusion. Altogether, our data suggest that a modulation of sphingosine maybe implicated in selective toxicity in VHL-deficient cells in response to STF-62247 and that thesecells could be target by lysosomal inhibitors.

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Title of the research project - Titre du projet de rechercheOn the Feasibility of Using Pattern Recognition Based Myoelectric Control as a Human-ComputerInterface for Individuals with Paralysis

Poster presenter - Nom de la personne qui présentera l'afficheMitchell Chaulk



Researchers involved in this project - Chercheurs participant au projetChaulk, Mitchell; Englehart, Kevin; Scheme, Erik; MacAlpine, Brenda; McCullum, Shane; O'Connel,Colleen.

Abstract - RésuméPattern recognition of the electromyogram (EMG) has long been employed as an effective controlsource for powered upper-limb prosthetics. The success that has been achieved, and theimprovements that are being made, are all in part due to the ability of pattern recognition basedmethods to extract relevant information from noisy, complex EMG signals. It therefor stands toreason that this solution could be applied to, and potentially benefit, patient populations other thanamputees. Neurological impairments often reduce a patient’s ability to control one or both of theirupper limbs. We hypothesize that EMG based pattern recognition approaches could characterizewhat motions are being attempted by a patient suffering from neurological impairment. This couldlead to novel rehabilitation solutions, or even restoration of limb function using functional electricalstimulation or powered orthotics. While there are many different sources of neurological impairment,this work focuses on incomplete spinal cord injury patients who are functional C4/C5 patients withASIA grades of A or B. The results of this work demonstrate the ability of these methods to classifyup to 10 different gestures, using 8 differential electrodes placed about a patient’s forearm. Theseresults suggest that EMG pattern recognition may provide an effective control portal for individualsaffected by C4/C5 spinal cord injury.

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Title of the research project - Titre du projet de rechercheProteomic approaches to study a small molecule targeting kidney cancer

Poster presenter - Nom de la personne qui présentera l'afficheNadia Bouhamdani



Researchers involved in this project - Chercheurs participant au projetBouhamdani, Nadia; Joy, Andrew; Barnett , David; Turcotte, Sandra.

Abstract - RésuméIntroduction. Le cancer du rein est l’un des cancers le plus à la hausse mondialement. En 2014, ilsera responsable de 6,000 nouveaux diagnostiques et 1,750 décès au Canada, le classant huitièmedans les cancers les plus diagnostiqués. L’inactivation du gène Von Hippel-Lindau (VHL) se produitlors du développement des carcinomes rénaux (RCC) dans plus de 80% des cas. Une petitemolécule, STF-62247 s’avère dix fois plus toxique chez les cellules déficientes en VHL et est utiliséecomme outil visant à cibler les RCC dont VHL est inactif. Objectifs. Nous avons évalué le profilglobal d’expression protéique afin de comprendre le mécanisme d’action du STF-62247. Méthode. «Stable Isotope Labeling by Amino acids in Cell culture » (SILAC) combiné à la spectrométrie demasse a été utilisé pour l’étude du protéome de cellules rénales cancéreuses traitées auSTF-62247. De plus, nous avons développé un protocole se servant de la propriétéauto-fluorescente de celle-ci afin d’identifier sa cible. Résultats. Environ 3000 protéines furentdifféremment exprimées après traitement au STF-62247, et 78% de celles-ci sont présentes dansun triplica biologique. La bioinformatique et des analyses de voies de signalisations ont mené à lavalidation de protéines affectées par la molécule. Un protocole de gel natif a permit de générer uneliste de cibles potentielles du STF-62247. Conclusion. L’identification des cibles de drogues est unoutil important menant au développement de thérapies plus sures. Les résultats de notre recherchepourraient mener au développement d’une nouvelle thérapie ciblée pour le traitement du cancerrénal.

Introduction. Kidney cancer is one of the fastest rising cancers worldwide. In 2014, it will beresponsible for 6,000 new diagnostics and 1,750 deaths in Canada, ranking it the eighth mostcommon cancer. Inactivation of the Von Hippel-Lindau (VHL) gene occurs in approximately 80% ofcases of renal carcinomas (RCC). We previously identified a small molecule, STF-62247, which hasbeen found to be ten times more toxic to VHL-deficient cells providing a selective approach to targetrenal cancer cells. Objectives. We evaluated global protein expression profiling to gain betterunderstanding of this small molecule, and aim to identify STF-62247’s target. Methods. StableIsotope Labeling by Amino acids in Cell culture (SILAC) combined with mass spectrometry has beenused to analyze the proteome of VHL-deficient cells in response to STF-62247. In addition, we usedthe auto-fluorescent property of the small molecule and developed a protocol to identify STF-62247potential targets. Results. About 3000 proteins were found to be differently expressed followingtreatment, and 78% were present in biological triplicates. Bioinformatics and data analysis pathwayslead to the validation of proteins affected by the small molecule. Then, a protocol using native gelsto separate protein generated a list of STF-62247 potential targets that bound to the small molecule

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and further investigations are necessary. Conclusion. Identifying targets of drugs is an importantstep towards the development of safer therapies. Outcomes from this research could lead to newtype of targeted therapies for the treatment of kidney cancer

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Title of the research project - Titre du projet de rechercheModeling and simulation of transfemoral Amputee gait

Poster presenter - Nom de la personne qui présentera l'afficheAbeer Mohamed



Researchers involved in this project - Chercheurs participant au projetMcGibbon, Chris; Mohamed, Abeer; Sexton, Andy; Simonsen, Kirsten.

Abstract - RésuméContemporary prosthetic solutions vary widely, from purely passive devices to micro-controlledpowered devices. However, controlling the prosthesis require extensive training sessions for theuser, and still relies on some manual operations by the user to ensure proper mode transitions. Thetrial-and-error nature of such training is burdensome for both the user and clinical team, thus limitingthe potential of this technology to be clinically adopted. A potential solution to this problem is todevelop a model-based platform that inputs subject-specific anatomy, biomechanics, and muscleelectrophysiology to simulate human movement and movement transitions of the user, which couldthen be used to pre-tune the prosthesis controller, greatly reducing the training burden. To test thissolution, our specific aims are: Aim 1: Adapt a currently existing neuromuscular model(23-degree-of-freedom, 92 muscle model) of the human upper and lower body to include anamputee's leg with a prosthesis. Aim 2: (a) Develop an efficient methodology for generatingmuscle-actuated simulation of human walking that closely reproduce experimental measures ofkinematic and ground reaction forces. (b) Acquire 3D motion analysis data and EMG from 16 limbedindividuals and 2 transfemoral amputees during a variety of locomotors activities to validate themodified model. Simulated joint kinematics closely tracked experimental quantities(mean-root-squared error generally less than 2mm), and the time histories of muscle activations wassimilar to electromyography recording. The results also showed that Forward Dynamic simulationsuccessfully tracked the set of desired kinematic and reproduced the same motion tracked byComputed Muscle control.The speed and accuracy of the Forward Dynamic and Computed MuscleControl make it practical to generate subject-specific simulations of gait.

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Title of the research project - Titre du projet de rechercheRegulation of autotaxin in hypertrophic cardiomyopathy

Poster presenter - Nom de la personne qui présentera l'afficheKenneth D'Souza


Education LevelHealth Professionals

Researchers involved in this project - Chercheurs participant au projetD'Souza, Kenneth; Lin, Chih_Wei; Weatherbee-Martin, Nathan; Trivedi, Purvi; Legare,Jean-Francois; Pulinilkunnil, Thomas; Kienesberger, Petra.

Abstract - RésuméBackground: Pathological cardiac hypertrophy is a growing health concern as it frequently leads toheart failure and malignant arrhythmia. The molecular mechanisms that underlie cardiachypertrophy remain poorly understood. Autotaxin (ATX) is a secreted enzyme that generates thebioactive lipid, lysophosphatidic acid (LPA), which binds to G protein-coupled receptors and exhibitsimportant biological functions. Few prior studies have suggested that LPA induces hypertrophicremodeling in cardiomyocytes. However, it remains unclear whether ATX is expressed incardiomyocytes and whether it plays a role in hypertrophic cardiomyopathy. Aims: The goal of thisstudy was to examine whether ATX is expressed in the heart and cardiomyocytes, and whether ATXexpression is regulated during hypertrophy. Methods and Results: Immunoblotting analyses of ATXprotein expression showed that ATX is expressed in the human and murine myocardium, humancultured cardiomyocytes, primary mouse cardiomyocytes, and H9C2 rat cardiomyoblasts. At leasttwo isoforms were detected (ATXα and β). Interestingly, cardiac ATXβ protein expression markedlyincreased in mice following stimulation of hypertrophy by transverse aortic constriction (TAC, 5weeks) and angiotensin II treatment (2.0 µg/kg/min, 3 days), when compared to controls. ATXprotein expression was also increased in differentiated H9C2 cardiomyoblasts following bothangiotensin II (1 µM) and phenylephrine (0.1 µM) treatment in parallel with the activation ofhypertrophy marker proteins. Taken together, these data suggest that ATX expression is increasedin whole hearts and cardiomyocytes following mechanical and pharmacological induction ofhypertrophy, and that increased ATX-mediated LPA production could play a role in the pathogenesisof hypertrophic cardiomyopathy.

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Title of the research project - Titre du projet de rechercheEffects of high intensity exercise training on anaerobic and aerobic contributions to total energyrelease during repeated supramaximal exercise in obese adults

Poster presenter - Nom de la personne qui présentera l'afficheHoria-Daniel Iancu



Researchers involved in this project - Chercheurs participant au projetIancu, Horia-Daniel; Paulin, Anne; Jabbour, Georges.

Abstract - RésuméBackground: Studying relative anaerobic and aerobic metabolism contributions to total energyrelease during exercise may be valuable in understanding exercise energetic demands and theenergetic adaptations that occur in response to acute or chronic exercise. Objective: The aim of thepresent study is to evaluate the effects of six weeks of high intensity exercise training (HIET) anddetermine the relative anaerobic and aerobic contributions to total energy release during repeatedsupramaximal cycling exercises (SCE) in obese adults. Design: Twenty four obese adults (bodymass index = ± 33 kg.m-2) were randomized into a control group (n=12) and an HIET group (n=12).Accumulated oxygen deficits (ml.min-1) and anaerobic and aerobic contributions (%) weremeasured in all groups before and after training via repeated SCE. Results: Before HIET, anaerobiccontributions to repeated SCE did not differ between the groups and decreased significantly duringthe 3rd and 4th repetitions. After HIET, anaerobic contributions increased significantly in the HIETgroup (+11%, p<0.01) and were significantly higher than those of the control group (p<0.01).Moreover, the peak power obtained during SCE increased significantly in the HIET group (+110W.kg-1, p<0.01) and correlated positively with increases in anaerobic contributions (r=0.9, p<0.01).Conclusion: In obese adults, HIET increased anaerobic contributions to energy release in responseto repeated SCE, which appears to be at least partially responsible for enhancing muscle power,even though no changes in free fat mass were observed.

Préambule: L'étude des contributions du métabolisme anaérobie et aérobie à la fournitureénergétique pendant l'exercice permet de connaitre les besoins énergétiques spécifiques et lesadaptations subséquentes en réponse à un exercice aigu ou chronique. Objectif: Le but de laprésente étude est d'évaluer les effets de six semaines d’entraînement à haute intensité (EHI) sur lacontribution de la voie anaérobie et aérobie à la fourniture énergétique lors d’un exercicesupramaximal répété sur ergocycle (ESR) chez les adultes obèses. Méthodes: Vingt-quatre adultesobèses (indice de masse corporelle = ±33 kg.m-2) ont été répartis aléatoirement entre deux groupes : contrôle (n =12) et EHI (n =12). Le déficit en oxygène accumulé en ml.min-1 et lacontribution des deux voies anaérobie et aérobie en pourcentage (%) ont été calculés pour les deuxgroupes avant et après l’EHI. Résultats: Avant l’EHI, la contribution anaérobie lors de l’ESR nediffère pas statistiquement entre les groupes. Toutefois, à la 3e et la 4e répétition celle-ci a diminuésignificativement (p<0,01). Après l’EHI, la contribution de la voie anaérobie a augmentésignificativement pour le groupe EHI (+11%, p <0,01). Cette contribution est significativement plusélevée comparé au groupe contrôle (p <0,01). De plus, la puissance pic obtenue lors de l’ESR a

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augmenté significativement pour le groupe EHI (+110 W. kg-1, p <0,01). Celle-ci était positivementcorrélée avec l'augmentation de la contribution anaérobie (r = 0,9, p <0,01). Conclusion : Chez lesadultes obèses, l’EHI a augmenté significativement la contribution anaérobie à la fournitureénergétique lors de l’ESR. Cette amélioration semble être responsable de l'amélioration de lapuissance musculaire, même si aucun changement dans la masse maigre n’a été observé.

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Title of the research project - Titre du projet de rechercheIs the province of New Brunswick limited in terms of physical activity resources? A pilot study

Poster presenter - Nom de la personne qui présentera l'afficheÉtienne Doucet


Niveau de formationÉtudiants de 1er cycle

Researchers involved in this project - Chercheurs participant au projetDoucet, Étienne; Iancu, Horia-Daniel; Leblanc, Roger G.; Jarbbour , Georges.

Abstract - RésuméAbstract - Résumé Background: Many strategies are used for health promotion and an associationhas been between the population’s overall health, physical activity (PA) and leisure resources. Inorder to optimize government action towards health promotion it is important to identify PA andleisure resources currently offered in a community. Objective: This pilot study aims to survey theresources for PA and leisure availabilities in the city of Moncton. Method: Five students wererecruited from the University of Moncton to identify all the PA and leisure activities available in thecity of Moncton. Data were collected using the official website of the city of Moncton and the websiteof the organizations offering activities. Once activities were collected they were categorized usingseveral criteria such as interior or exterior activities, cost of participation and if there weresupervised. Actually, these criteria are known to affect the physical activity practices. Results: InMoncton, 150 resources have been identified. Of these, 85% have a participation cost associated tothem, 72% are indoor and 12% are outdoor, 74% are supervised and 26% are unsupervised. Conclusion: In Moncton, 48% of individuals aged 15 and over have a considerable low income andalso only 46.5% of New-Brunswickers have a level 3 standard of literacy. Then, our results maysuggest that in the case of the City of Moncton the accessibility of families to some resources maybe limited.

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Title of the research project - Titre du projet de rechercheThe Impact of Geographic Factors on Access to Cardiac Catheterization in New Brunswick

Poster presenter - Nom de la personne qui présentera l'afficheAnsar Hassan



Researchers involved in this project - Chercheurs participant au projetHassan, Ansar; Yip, Alexandra M.; MacLeod, Jeffrey B.; Murray, Joshua; Lutchmedial, Sohrab;Pelletier, Marc P..

Abstract - RésuméBackground: Previous studies have demonstrated the deleterious effect of geographic factors onaccess to cardiac care across Canada. This study examined the contemporary effect of geographicfactors on access to cardiac catheterization in New Brunswick. Methods: All patients undergoingcardiac catheterization at the New Brunswick Heart Centre (NBHC) between October 2005 andJune 2011 were identified. Postal code was used to calculate distance from the patient’s home tothe NBHC (0-50km, 50-150km, 150-250km, and >250km). Access to cardiac catheterization wasdefined as wait time from referral date to procedure date. Comparisons across distance categorieswere conducted by baseline clinical characteristics (age, sex, urgency), socio-geographic factors(social and material deprivation, urban vs. rural, regional health authority, distance to nearesthospital) and wait times. Cox proportional hazard modeling determined the risk-adjusted effect ofincreased distance from the NBHC on access. Analyses were carried out separately for inpatientand outpatient procedures. Results: 18,427 patients formed the final study population (inpatient:n=11,412; outpatient: n=7015). Following risk-adjustment, increased distance emerged as anindependent predictor of reduced likelihood of cardiac catheterization over time for inpatients[0-50km: HR 1.00 (referent); 50-150km: HR 0.44 (95% CI: 0.42-0.46); 150-250km: HR 0.44 (95% CI0.41-0.47); >250km: HR 0.37 (95% CI 0.35-0.39)] and outpatients [0-50km: HR 1.00 (referent);50-150km: HR 0.86 (95% CI: 0.80-0.92); 150-250km: HR 0.87 (95% CI 0.81-0.94); >250km: HR0.81 (95% CI 0.75-0.88)]. Conclusion: Geographic factors continue to affect access to cardiaccare in Canada. Further work is required to better understand and ultimately eliminate thisrelationship.

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Title of the research project - Titre du projet de rechercheComparing Access to Primary Health Care among New and Traditional Models:

Poster presenter - Nom de la personne qui présentera l'afficheAshley Thompson



Researchers involved in this project - Chercheurs participant au projetMiedema, Baukje; Easley, Julie; Thompson, Ashley; Wong, Sabrina; Wochis, Walter; Hogg, William;Breton, Mylaine; Burge, Fred.

Abstract - RésuméMany Canadian provinces have established new models of PC (NMPC) that are team based andcharacterized by alternatively funded, collaborative, inter-professional principles. Conversely,traditional models of PC (TMPC) are typically single physician practices. An international surveyexamining the Quality and Cost of Primary Care (QUALICOPC) was adapted in 2013 to collectCanadian data. This data was used in the current presentation to investigate differences in PCaccess between NMPC and TMPC from patients’ perspectives. The final sample included 759 PCproviders and 7,172 patients from 736 practices across 10 provinces. Physician and patient datafrom QUALICOPC surveys were used to measure dimensions of PC access (e.g., physical andfinancial, scheduling, distance to practice, other health care providers). The majority of patientsdescribed using PC more than four times annually: 38%/4+ times and 20%/10+ times; the majorityof these visits were non-urgent. Patients reported PC was “easily accessible” regardless of themodel of care. Results from multiple ANCOVAs revealed no differences in patients’ reports ofphysical, financial, or travel/distance issues regarding access to care between the two PC models.However, patients attending practices using TMPC reported significantly fewer scheduling issuesbut greater barriers accessing other health care providers than patients in NMPC. Overall, patientsvisited their PC provider frequently for non-urgent issues, and perceived that their PC provider waseasily accessible. As expected, patients in NMPC reported easier access to other health careproviders than patients in TMPC. However, patients in NMPC experienced more scheduling issuesthan patients in TMPC.

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Title of the research project - Titre du projet de rechercheAccountability of Mental Health Services in non-Profit Organizations: Examining the Link betweenMandates and Outcomes in New Brunswick

Poster presenter - Nom de la personne qui présentera l'afficheIsabel Garcés Dávila, M.A. Candidate, University of New Brunswick



Researchers involved in this project - Chercheurs participant au projetRonis, Scott.

Abstract - RésuméNon-profit organizations are increasingly being pressed to measure and report their outcomes tostakeholders and other constituents; pressure from government is becoming more significant asnon-profit organizations progressively engage in the provision of government funded services(Moxham, 2008). As such, this study examined the association between perceived transparency oforganizational mandates and outcome measurement in non-profit organizations offering youthmental health services. Specifically, we examined whether outcome measurement andorganizational confidence is linked with perceived importance of examining key indicators from theStudent Wellness Survey. Type of services provided, location of organization and type of populationserved were also included in the analyses. Service providers from non-profit organizations servingyouth mental health were asked to complete an online survey regarding the mandates andoutcomes of each corresponding agency. Results showed that 54% of non-profit organizationscollect outcomes. In addition, 54% of service providers believe their organization has clearmandates and goals, and 40% believe their organization adequately meets the needs of thepopulation it serves. Findings from this study suggest that there are significant associations betweenmandates and outcome measurement in non-profit organizations. In addition, collecting outcomescan be associated with funding opportunities, partnerships and type of services provided. Findingsfrom this study can help researchers understand the accountability process in non-profitorganizations offering mental health services. Recommendations for future steps are provided tobetter examine outcomes in non-profit organizations.

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Title of the research project - Titre du projet de rechercheAssessing quantitative PCR and nested PCR protocols for detection of Borrelia burgdorferi, theLyme disease bacteria

Poster presenter - Nom de la personne qui présentera l'afficheKami Harris



Researchers involved in this project - Chercheurs participant au projetHarris, Kami; Filiaggi, Corey; Lloyd, Vett.

Abstract - RésuméLyme borreliosis (LB), commonly known as Lyme disease, is caused by an infection with thespirochaete bacteria Borrelia burgdorferi and is primarily transmitted by Ixodes ticks. Symptoms ofLB are difficult to diagnose as they vary and are non-specific. When a tick is found feeding, testingit for the presence of B. burgdorferi is one of the best ways to assess the risk of infection. NestedPCR (nPCR) and quantitative PCR (qPCR) are the two commonly used ways to test the ticks. Thegoal of this study is to assess these two methodologies for sensitivity and accuracy. Engorged andnon-engoraged non-infected tick DNA was spiked with varying amounts of pure B. burgdorferigenome to determine the limit of detection in each protocol. Error rate (false positives andnegatives) was determined by the consensus of repeat amplifications of the same sample. It wasfound that nPCR is more sensitive then qPCR but, has a higher rate of false positives. nPCR is theideal method of testing in a research setting when repeat amplifications can exclude false positives;however, qPCR is superior for large scale surveillance due to its convenience and speed. Thesefindings are significant because it is important to understand the limits of testing methodologies thatare used as part of a clinical diagnosis.

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Title of the research project - Titre du projet de rechercheAntihypertensive effect of caffeic acid analogs through dual renin-angiotensin-aldosterone systeminhibition

Poster presenter - Nom de la personne qui présentera l'afficheMohamed Touaibia



Researchers involved in this project - Chercheurs participant au projetTouaibia, Mohamed; Lassalle-Claux, Grégoire ; Bhullar, Khushwant S.; Rupasinghe, H.P.Vasantha.

Abstract - RésuméHypertension is a crucial risk factor for cardiovascular diseases and contributes to one third of globalmortality. In addition to conventional antihypertensive drugs such as captopril, naturally occurringphytochemicals and their analogs are used for reducing the risk and occurrence ofhypertension.Herein, we demonstrate the possible use of caffeic acid and its derivatives in thetreatment of hypertension through multi-target modulation of renin-angiotensin-aldosterone system(RAAS). Caffeic acid along with its nineteen novel derivatives, chlorogenic acid,quercetin andcaptopril were all investigated for the inhibition of renin and angiotens in converting enzyme (ACE)activities and production of aldosterone. Compound 22 with CH2CH(Ph)2 moiety exhibited thestrongest renin inhibition (IC50 = 229 mM) among all compounds tested. Caffeic acid was theweakest renin inhibitor (IC50 = 5704 mM) among all the compounds assayed.Similar torenininhibition, compound 22 (IC50 = 9.1 mM) also exhibited about 47 times stronger ACE inhibitioncompared to the parent compound. Analysis of aldosterone revealed that compound 8 with n-Prmoiety was the strongest modulator of aldosterone production among all the derivatives. Toxicityanalysis using human fibroblasts (WI-38cells) confirmed thenon-toxic manifestations of caffeic acidand its derivatives in comparison to clinically used drug captopril.

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Title of the research project - Titre du projet de rechercheFood related worries and body image issues in student athletes: A photovoice exploration.

Poster presenter - Nom de la personne qui présentera l'afficheSamantha Stewart


Education LevelUndergraduate Students

Researchers involved in this project - Chercheurs participant au projetStewart, Samantha; Hamilton, Ryan.

Abstract - RésuméThe purpose of this study was to examine the concept of anxiety, what it looks like, what it feels like,and what it means to athletes who are experiencing it. There is a gap that exists within anxietyliterature in sport resulting from the vast majority of research examining anxiety that has quantified asubjective experience. Missing from our understanding of anxiety in athletics is a sense of how it isexperienced by the athlete. We have an understanding of what factors influence anxiety and howanxiety can be detrimental to sport performance; however, we are missing how it feels. Without suchan understanding, we are left with an inadequate analysis of a dynamic and universal phenomenon.Employing the Photovoice method, this study utilized photography and semi-structured interviews tohave athletes capture what anxiety is to them and explain their personal experiences. Thematicanalysis in conjunction with examination of the participants’ photographs was performed to findcommon themes among athletes’ experiences with anxiety and capture the essence of theseathletes’ stories. Six major themes were identified and they had to do with scheduling/managingathletics and school, expectations for both performance and behaviour, food related worries,managing injuries, body image issues, and small worries that are amplified due to alreadyheightened competition anxiety. Mental health treatment implications for student athletes strugglingwith anxiety [food related issues and body image issues] will be discussed.

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Title of the research project - Titre du projet de rechercheA subcellular fractionation method to enrich endoplasmic reticulum from Saccharomyces cerevisiaeand human fibroblasts with ALG9 mutation

Poster presenter - Nom de la personne qui présentera l'afficheAlyson Zwicker and Tess Robart



Researchers involved in this project - Chercheurs participant au projetZwicker, Alyson; Robart, Tess; Nadaradjan, Rattina Dasse; Martyniuk, Chris; Taylor, Sherry A.;Kienesberger, Petra; Webster, Duncan; Pulinilkunnil, Thomas.

Abstract - RésuméBackground: Glycosylation is an enzymatic process wherein sugars are added to proteins, formingcomplex glycoproteins that are vital for cellular functioning. During the N-glycosylation process,α1,2-mannosyltransferase protein known as ALG9 facilitates the addition of mannose residues to agrowing oligosaccharide chain, which is subsequently added to nascent polypeptides in theendoplasmic reticulum (ER). Faulty glycosylation secondary to mutations in ALG9 gene result inCDG-IL, a rare subtype of Congenital Disorders of Glycosylation (CDG) characterized by seizures,psychomotor retardation and brain atrophy. Aim: The goal of this study was to develop a robustsubcellular fractionation protocol to isolate the ER that may be utilized for further functional analysisof human ALG9 in vitro. Methods and Results: This method was validated in dermal fibroblasts froma patient with an ALG9 mutation and in yeast deficient for ALG9 or harboring wild type or mutanthuman ALG9. To separate purified ER, differential centrifugation and discontinuous sucrosegradients were applied to the cellular pellet. Total protein lysate and purified ER fractions weresubjected to immunoblot analysis to confirm enrichment of ER associated proteins. ER fractionsfrom human fibroblasts and WT and mutant yeast strains exhibited enrichment of dolichol-phosphatemannose synthase (Dol-P Man), a shuttle protein employed to transport mannose into the ER.Notably, deficiency/mutation of ALG9 correlated with hypo-glycosylation of carboxypeptidase Y(CPY), a yeast vacuolar protein synthesized in the ER. Significance: In summary we havedeveloped a valuable method to enrich ER which can be employed for studying ALG biology andfurthering CDG research.

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Title of the research project - Titre du projet de recherche"The risk of Borrelia infection (Lyme disease) in New Brunswick - using dogs as a sentinel speciesto assess the risk to humans"

Poster presenter - Nom de la personne qui présentera l'afficheNatalie Bjurman



Researchers involved in this project - Chercheurs participant au projetFiliaggi, Corey; Lloyd, Dr. Vett.

Abstract - RésuméBackground: Lyme borreliosis (LB; Lyme disease) is caused by infection with the spirochete bacteriaBorrelia burgdorferi and transmitted by the bite of an Ixodes tick. Untreated LD can lead tolong-term debilitating chronic illness. LB is the most commonly arthropod vectored disease in NorthAmerica, and in the U.S. the risk of infection is now 6 times greater than that of HIV. Our preliminarywork shows a high percentage of Borrelia-infected ticks throughout New Brunswick,. Hypothesis:Canine seropositivity rates in NB will allow an estimate of the risk of disease to humans living in thesame communities. Methodology: As previous studies have shown human infection rates to be1/6th that of dogs, the use of canines as a sentinel species is a useful method of assessing the riskto humans. Approximately 700 blood samples were collected, 100 from each of the 7 health districtsin NB over the fall and spring of 2013/2014. Samples were tested for Borrelia seropositivity using acommercial C6 ELISA test (IDEXX Snap4DX+) . Results: Preliminary data shows an overallprovincial infection rate of 7%, with infection rates as high as 17% in the southernmost regions ofthe province. Seropositive dogs have been found throughout the province. Significance: As a studyconducted 3 years ago found that fewer than 1% of NB dogs were infected. NB has experienced arapid increase in the frequency and distribution of infected ticks, showing there is now anappreciable risk of LB throughout the province.

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Title of the research project - Titre du projet de rechercheCharacterization of Novel miRNAs Involved in Breast Cancer Metastasis

Poster presenter - Nom de la personne qui présentera l'afficheDominique Comeau



Researchers involved in this project - Chercheurs participant au projet.

Abstract - RésuméMicroRNAs (miRNAs) have been found to play key roles in breast cancer progression, notablyduring epithelial-to-mesenchymal transition (EMT) and metastasis. It has also been proposed thatmiRNAs could be used as blood-bourne biomarkers for the early detection of metastasis. To gain abetter understanding of the miRNAs that are involved in breast cancer metastasis, miRNA profilingof the immortalized breast epithelial cell line MCF-10A and its mesenchymal counterpart MCF-10AMby Next-generation sequencing (NGS) was performed, which revealed 20 upregulated potentialoncogenic miRNAs (oncomirs) and 16 downregulated potential tumour suppressor miRNAs. Weperformed qRT-PCR analysis of these potential EMT-regulating miRNA to validate theirdysregulation in MCF-10AM cells. The list of dysregulated miRNA was narrowed down to 4 upregulated miRNAs (miR100, miR22, miR152 and 891a) and 4 downregulated miRNAs (miR33,miR27a, miR378a and miR378d) in MCF-10AM cells compared to MCF-10A cells. The expressionof these miRNAs was subsequently probed in other established epithelial (MCF-7) andmesenchymal (MDA-MB-231) cell lines. The dysregulated expression of five miRNA was validatedby this approach: miR22, miR100, miR378a, miR378d and miR33. Each of these miRNAs do nothave any reported role in cancer, therefore we believe that these miRNA may represent novelEMT-regulating miRNAs. Experiments are presently being performed to characterize their role in theregulation of EMT.

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Title of the research project - Titre du projet de rechercheAssessing the potential of Ixodes cookei as a vector for Lyme Disease

Poster presenter - Nom de la personne qui présentera l'afficheJamie Patterson



Researchers involved in this project - Chercheurs participant au projetPatterson, Jamie; Duncan, Anna; Lloyd, Vett.

Abstract - RésuméBackground: Lyme borreliosis, commonly know as Lyme disease, is an emerging disease in NewBrunswick. The disease is caused by infection with the bacteria Borrelia burgdorferi. Lyme diseaseis vectored by ticks, of which there are several species established in this region. The onlyrecognized vector in eastern Canada is Ixodes scapularis, commonly known as the black-leggedtick. Hypothesis: Ixodes cookei, the groundhog tick, a closely related and well-established speciesin NB, is involved in the spread of Lyme disease. Methods: Passive surveillance of tick populationsin NB, and DNA analysis of ticks for Borrelia infection and species identification. Results: I. cookeihave been repeatedly found feeding on humans and companion animals. I. cookei are found to beinfected with Borrelia and the infection rate is comparable to that of I. scapularis. Further, ticks withmorphological intermediate between Ixodes scapularis and Ixodes cookei are present. Of 28 tickstested, one showed clear evidence of hybridization. Significance: While neither the hybrids nor thepure I. cookei have been directly tested for their ability to transmit Borrelia, these ticks do carryBorrelia and feed from humans. There is a case report of I. cookei causing Lyme disease in the US,so the evidence suggests they should be considered as a vector for Lyme disease.

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Title of the research project - Titre du projet de rechercheValidation of two next-generation sequencing cancer panels for clinical testing

Poster presenter - Nom de la personne qui présentera l'afficheNicolas Crapoulet



Researchers involved in this project - Chercheurs participant au projetCrapoulet, nicolas; Caissie, Gilberte; Richard, Luc; Maicas, Emmanuel; Ouellette, Rodney.

Abstract - RésuméWith their high throughput and low operating cost, next-generation sequencers (NGSs) have quicklybecome tools of choice for simultaneous sequencing of multiple genes from different patients and,as a result, an attractive solution for analyzing large volumes of samples in the diagnostic laboratoryenvironment. Moreover, the flexible design of multigene tests conducted with these NGSs makesthe clinical testing process modular by extending analysis to novel genes and polymorphisms thatcannot be covered by routine tests. Following its acquisition of PGM NGSs from Life Technologies in2012, the Atlantic Cancer Research Institute has been working closely with the molecular geneticslaboratory at Dr. Georges-L.-Dumont University Hospital Centre toward offering new diagnostic testsfor various diseases including cancer. Objective: We evaluated two next generation sequencingassays from Ion Torrent, the AmpliSeq Cancer Hotspot Panel V2 and the AmpliSeq Lung and ColonCancer Panel, capable of identifying multiple somatic mutations in 50 genes in a single assay and22 genes respectively. We demonstrated a 100% concordance in each formalin-fixedparaffin-embedded (FFPE) tissue sample previously tested and the corresponding variants identifiedusing both Ion Torrent panels. Serial dilutions of DNA mixture extracted from various cell lines aswell as genetically engineered cell lines in FFPE demonstrated that the limit of detection for bothpanels is validated at 5% for single nucleotide variants. Conclusions: We have evaluated theperformance of the AmpliSeq Cancer Hotspot Panel V2 and the AmpliSeq Lung and Colon CancerPanel and shown that they are suitable for clinical testing.

Grâce à leur débit élevé et leurs coûts d'exploitation faibles, les séquenceurs de nouvelle génération(SNG) sont rapidement devenues des outils de choix pour le séquençage simultané de plusieursgènes provenant de différents patients et, par conséquent, une solution intéressante pour l'analysede grands volumes d'échantillons dans les laboratoires de diagnostic moléculaire. En outre, laconception flexible de tests multigéniques menée avec ces SNGs rend le processus de test cliniquemodulable en étendant l'analyse de nouveaux gènes et des polymorphismes qui ne peuvent êtrecouverts par des tests de routine. Suite à l’acquisition d’un séquenceur nouvelle génération PGM deLife Technologies en 2012, l’Institut Atlantique de Recherche sur le Cancer (IARC) a travaillé enétroite collaboration avec le laboratoire de génétique moléculaire du centre hospitalier universitaireDr-Georges-L.-Dumont afin d’offrir de nouveaux tests de diagnostic pour les diverses maladies dontle cancer. Objectif: Nous avons évalué deux tests diagnostiques utilisant le SNG de Ion Torrent, lepanel AmpliSeq cancer Hotspot V2 et le panel AmpliSeq cancer du poumon et du côlon, capablesd'identifier plusieurs mutations somatiques dans 50 gènes simultanément et 22 gènesrespectivement. Nous avons démontré une concordance de 100% entre les variants précédemment

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identifiés dans des échantillons de tissus FFPE et les variants identifiés en utilisant les deux panelsIon Torrent. Des dilutions en série de mélange d’ADN extrait à partir de différentes lignéescellulaires ainsi que des tissus FFPE de lignées cellulaires génétiquement modifiées ont montré quela limite de détection pour les deux panels est validée à 5% pour des variants d'un seul nucléotide. Conclusions: Nous avons évalué la performance du panel AmpliSeq cancer Hotspot V2 et du panelAmpliSeq cancer du poumon et du côlon et nous avons montré qu'ils étaient adaptés pour lesessais cliniques.

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Title of the research project - Titre du projet de rechercheExperiences with chronic undiagnosed illness in Canada

Poster presenter - Nom de la personne qui présentera l'afficheCorinne Boudreau



Researchers involved in this project - Chercheurs participant au projetBoudreau, Corinne; Lloyd, Vett.

Abstract - RésuméLyme disease is a growing medical concern in the Maritime Provinces. A complex illness, recentfindings suggest that a large number of Lyme disease cases have escaped diagnosis. Additionally,disagreement exists as to the best method of treating the disease, with the result that manyCanadians with Lyme disease are seeking treatment outside of the conventional Canadian HealthCare system. This may include long-term antibiotic treatment offered in the United States as well asalternative treatments. As these patients are treated outside the conventional Canadian medicalsystem, their motivations and experiences have not been documented, nor is there systematicmonitoring of the effectiveness of these treatments. Our research involved personal interviews withindividuals diagnosed with Lyme disease who have sought treatment outside of the CanadianHealthcare system. Individuals were recruited primarily through Lyme disease support groups, soparticipants who received prompt care for acute Lyme in Canada were under-represented. Weinvestigated their experiences in obtaining diagnosis and treatment for Lyme disease and collectedtheir perceptions as to how health parameters differed before and after treatment. Manyrespondents reported extensive testing, delayed diagnosis and expressed frustration with theCanadian medical community, leading to their search for treatment outside of Canada. Additionally,many reported that the combination of escalating health concerns and lack of diagnosis producedsevere emotional effects, including anger, hopelessness and depression. Self-financed healthcarefrequently resulted in financial strain. Most respondents reported a perceived improvement insymptoms with long-term antibiotic treatment or other alternative care.

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Title of the research project - Titre du projet de rechercheInstrumented pendulum test for objective spasticity assessment of the knee

Poster presenter - Nom de la personne qui présentera l'afficheLeblanc, Melanie

First author - Premier auteurNo


Researchers involved in this project - Chercheurs participant au projetLeblanc, Melanie; Sexton, Andrew; O'Connell, Colleen; McGibbon, Chris.

Abstract - RésuméPeople with multiple sclerosis (MS), cerebral palsy (CP), acquired brain injury (ABI) and spinal cordinjury (SCI) often experience persistent muscle spasticity, characterized by involuntary musclecontraction when stretched, which interferes with functioning and is often painful. Spasticitymanagement through pharmacologic intervention is common, but is costly and can result innumerous side-effects. Accurate assessment of spasticity is critical for ensuring the efficacy of thesetreatments. The current standard for spasticity assessment is the Modified Ashworth Scale (MAS),requiring the therapist to rapidly rotate the joint through its range of motion, and record the degree ofmuscle resistance encountered on a 5-point scale. The MAS can be administered quickly andefficiently, but has been widely criticized due to its subjective nature. An alternative approach is theWartenberg pendulum test, which requires the patient to be seated and the therapist to hold anddrop the lower leg, and observe the limb’s oscillations. These observations can be made objectivelyusing a wearable sensor system that quantifies the degree of spasticity based on a pendulum testoutput called the relaxation index (RI). We evaluated the pendulum test for assessing kneespasticity in four groups of patients (MS, CP, ABI and SCI) relative to healthy age-matched controls,and correlated the RI score with MAS score. Overall, RI scores explained 42% of the variance inMAS scores, and mean RI for all patient groups was significantly different from healthy controls(p<.05). However, RI variance increased with higher tone (MAS>2) thus requiring more cautiousinterpretation.

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Title of the research project - Titre du projet de rechercheDetermining the utility of mobile applications for managing blood-iron in hemochromatosis treatment

Poster presenter - Nom de la personne qui présentera l'afficheAndrew Hamilton-Wright

First author - Premier auteurNo


Researchers involved in this project - Chercheurs participant au projetGrewal, Gary; Hamilton-Wright, Andrew; Carter, John; D'Angelo, Andrew; Liu, Jiexin Frank; van derLaan, Sarah; Alexander, Thomas.

Abstract - RésuméThe single most common genetic disorder affecting Canadians is hemochromatosis, a geneticdisorder that causes dangerous dietary iron buildup within the body, and can lead to liver and heartdisease, as well as diabetes. Currently, there is insufficient information available to model the likelytime before a patient will return to good health after treatment begins, and in addition, patients haveno easy way of personally monitoring their progress and managing their appointments for treatment. We have created a free mobile App for both Android and Apple devices to allow patients tovisualize and manage their data. Downloads are tallied in order to determine interest and estimatethe size of a potential participant pool in a data sharing study. Over 600 downloads of the tool havebeen performed to date, over the course of roughly two months. It appears that information aboutthe app is propagating by word of mouth, as indicated by the sporadic burst behaviour in downloads. Australia currently has the largest number of downloads, indicating saturation of the interestedparticipant pool has not yet occurred, based on relative population. The international response tothis project has uncovered further complexity in the modelling problem, as different jurisdictions(e.g.; Australia) are using different blood protein based methods to track iron levels. Overall, thisindicates that availability of a group of significant size is available from whom it may be possible toobtain data samples in order to model treatment outcomes.

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Title of the research project - Titre du projet de rechercheA Naturalistic Study of Paliperidone Palmitate in Patients with Schizophrenia: Methods of theCanadian Sustenna Treatment Assessment Registry (CanSTAR)

Poster presenter - Nom de la personne qui présentera l'afficheMichael Kemp



Researchers involved in this project - Chercheurs participant au projetKemp, Michael; Awad, A. George; Camacho, Fernando; Chue, Pierre; Demers, Marie-France;Quinn, Anne Marie; Raedler, Thomas; Streiner, David.

Abstract - RésuméBackground: Clinicians adopt various strategies in real-world practice to manage and preventsymptom exacerbation, including relapse, for individuals with schizophrenia. Well-designednaturalistic studies provide complementary data to randomized controlled trials and are increasinglyrecognized as practice-informing by the medical community. Purpose: To estimate relapse rate ofadult patients with schizophrenia in Canada who receive the second generation long-actingantipsychotic injection (SGA-LAI), paliperidone palmitate (PLAI), over a 12-month period. Methods: This is an open-label, non-controlled, multicenter, naturalistic study in patients with schizophrenia(18-65 years). Eligible patients (N=250) include those treated with PLAI who are converting fromoral antipsychotics under routine clinical care and can provide informed consent. Can-STARcontains minimal eligibility criteria enabling the enrollment of a heterogeneous population to reflecteveryday practice. Time and events include: treatment initiation visits at days 1 and 8, followed by12 monthly treatment visits (based on the recommended labeled dosing of PLAI) with assessmentscompleted every 3 months. Results: Clinical and functional outcomes will be assessed usingdescriptive methods, including symptomatic relapse based on Csernansky criteria, hospitalization,CGI-S, GAF, EQ5D, PETiT, MSQ, MPQP and resource utilization. Safety will be monitored. Characterization of co-morbidities, substance use/abuse, and concomitant use of psychotropicmedications will be described. No formal hypothesis will be tested. Discussion: Findings willprovide information regarding the clinical management of schizophrenia, the relapse profile of aSGA-LAI in a real world setting and specifically explore the utilization of PLAI in Canada. Limitationsinclude the absence of a controlled group for descriptive comparisons.

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Title of the research project - Titre du projet de rechercheDecreased eIF3e expression can mediate epithelial-to-mesenchymal transition through activation ofthe TGF β signaling pathway

Poster presenter - Nom de la personne qui présentera l'afficheGuillaume Desnoyers



Researchers involved in this project - Chercheurs participant au projetDesnoyers, Guillaume; Frost, Laura; Lewis, Stephen.

Abstract - RésuméBackground The eIF3e protein is a component of the multi-subunit eIF3 complex, which is essentialfor cap-dependent translation initiation. Decreased eIF3e expression is often observed in breast andlung cancer and has been shown to induce epithelial-to-mesenchymal transition (EMT) in breastepithelial cells by an unknown mechanism. Here, we study the effect of decreased eIF3e expressionin lung epithelial cells and explore the mechanism involved in the induction of EMT by decreasedeIF3e expression in both breast and lung epithelial cells. Results We created stable clones of lungepithelial cells (A549) that express an eIF3e-targeting shRNA and studied the molecular mechanisminvolved in eIF3e-regulated EMT in both breast and lung epithelial cells using a series of molecularand cell biology methods. Our data indicate that decreased eIF3e expression in lung epithelial cellsleads to EMT, as it does in breast epithelial cells. Importantly, we show that decreased eIF3eexpression in both lung and breast epithelial cells leads to the overproduction of the TGF-β cytokineand that inhibition of TGF-β signaling can reverse eIF3e-regulated EMT in lung epithelial cells. Inaddition, we discovered several mRNAs that encode important EMT regulators are translated by acap-independent mechanism when eIF3e levels are reduced. Conclusions We find that EMTmediated by a decrease in eIF3e expression may be a general phenomenon in epithelial cells. Wefurther find that EMT in lung epithelial cells that is caused by a decrease in eIF3e expressionrequires activation and maintenance of the TGF- signaling pathway.

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Title of the research project - Titre du projet de rechercheDiet induced obesity and type 2 diabetes impairs lysosomal autophagy in the murine myocardium

Poster presenter - Nom de la personne qui présentera l'afficheTRIVEDI PURVI



Researchers involved in this project - Chercheurs participant au projetTRIVEDI, PURVI; WEATHERBEE-MARTIN, NATHAN; KIENESBERGER , PETRA;PULINILKUNNIL , THOMAS.

Abstract - RésuméBackground: Preclinical and clinical data show that obese and diabetic hearts are susceptible tomitochondrial and endoplasmic reticulum (ER) dysfunction. ER directs misfolded proteins towardsdegradation, which occurs not only in the proteasome but also in the lysosome by chaperonemediate autophagy (CMA). However, it is unknown if lysosomal protein degradation is impaired inthe obese and diabetic myocardium. The rate limiting step for CMA activation is binding oflysosome-associated membrane protein-type 2A (LAMP-2A) to proteins targeted for degradation. Aim: We hypothesized that a reduction in LAMP-2A and CMA, renders the obese and diabetic heartsusceptible to proteotoxicity induced cardiac dysfunction. Methods and Results: To address the roleof lysosomal CMA in the obese and diabetic heart, C57BL6 mice were fed either regular chow (4%kcal from fat) or high fat-high sucrose (HFHS) diet (45% kcal from fat) for 15 weeks. HFHS fed miceexhibited insulin resistance, hyperglycemia, cardiac lipotoxicity and myocardial dysfunction.Immunoblot analysis revealed a significant decrease in autophagy markers namely LC3-B,LAMP-2A and Hsc70. Nuclear transcription factor EB (TFEB), which induces lysosomal biogenesis,was significantly decreased in hearts of HFHS fed mice with concomitant increases in proteinexpression of phosphorylated mammalian target of rapamycin (mTOR), S6 kinase and S6,suggesting hyper-activation of mTOR signaling. Since mTOR is the endogenous inhibitor ofautophagy, our data suggest that in the obese and diabetic heart, hyper activation of mTOR inhibitsLAMP-2A and TFEB. Significance: Impaired lysosomal biogenesis and CMA during obesity anddiabetes renders the heart susceptible to failure.

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Title of the research project - Titre du projet de rechercheTrends in incidence and prevalence of heart failure in New Brunswick

Poster presenter - Nom de la personne qui présentera l'afficheHao Wang



Researchers involved in this project - Chercheurs participant au projetWang, Hao; Collette, Maurice; Pilgrim, Wilfred; Gupta, Neeru.

Abstract - RésuméHeart disease is one of the leading causes of hospitalization and a major contributor to health carecosts in this province. This study looks at heart failure (HF), the second most common form of heartdisease, to better understand one aspect of heart health in NB. Methods: Data from the CanadianChronic Disease Surveillance System were used in this study and are based on hospital dischargerecords. Incidence and prevalence rates were calculated by sex and fiscal year. Results: Theincidence rate of NB adults aged 40+ being hospitalized for new onset of HF decreased by about35% from 2000-01 to 2009-10. The prevalence of any hospitalization for HF decreased by about 5%over the same period. Age-adjusted incidence and prevalence rates varied significantly amongzones. Discussion: While the incidence of HF is decreasing, the demands on our health caresystem remain high because people are living longer with the disease. With the expected rapidincrease in the proportion of seniors in the coming years, the evidence is inconclusive as to whetherthe declining trend in incidence will persist. A shift in preventive efforts that focus solely on changingindividual behaviours to those that address the underlying factors and systems that contribute topopulation heart health and reduce inequities is essential to improve the hearth health of NewBrunswickers. In addition to analyzing trends in disease incidence and prevalence,evidence-informed population health approaches will require examination of the associationsbetween social determinants of health and heart health in this province.

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Title of the research project - Titre du projet de recherche17β-estradiol-induced breast cancer cell proliferation requires stearoyl-CoA desaturase-1expression and activity

Poster presenter - Nom de la personne qui présentera l'afficheAnissa Belkaid



Researchers involved in this project - Chercheurs participant au projetBelkaid, Anissa; Ouellette, Rodney; O; Surette, Marc; E.

Abstract - RésuméIntroduction: Cancer cells face their increased requirements for membrane biogenesis by increasinglipogenesis. Stearoyl-CoA desaturase-1 (SCD-1), a lipogenic enzyme that transforms saturated(SFA) to monounsaturated fatty acids (MUFA), is required for cell proliferation in many cancer celltypes. However, estrogen that drives proliferation of most breast cancers is a known suppressor ofSCD-1 in adipocytes and hepatocytes. In this study, we sought to elucidate the involvement ofSCD-1 in the estrogen-induced mitogenic growth of breast carcinoma cells. Methodology andresults: MCF-7 and T47D breast carcinoma cells and normal immortalized MCF-10A mammaryepithelial cells were hormone-starved and then incubated or not with 17β-estradiol (17β-ED).17β-ED induced cell proliferation and increased MUFA/SFA ratios, indicative of SCD-1 activity, inMCF-7 and T47D cells but not MCF-10A cells. Accordingly, enhanced cellular proliferation wasaccompanied by increased SCD-1 expression measured by qPCR, western blot andimmunohistochemistry that was accompanied by an increase in the mature form of SREBP-1, atranscription factor that regulates SCD-1. Incubation of MCF-7 cells with a SCD-1 inhibitor(A939572) or SCD-1 silencing with siRNA blocked proliferation and prevented changes in cellularlipids suggesting that SCD-1 is essential for estrogen-induced proliferation. Incubation of cells withan IGF-1 receptor antagonist (AG1024) partially blocked 17β-ED-induced proliferation and SCD-1expression suggesting that the 17β-ED-induced SCD-1 expression is partially mediated thoughIGF-1R signaling. Conclusions: This study demonstrates for the first time that estrogen can act likean activator of SCD-1 in breast cancer cell. These results support SCD-1 as a therapeutic target inestrogen-sensitive breast cancer.

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Title of the research project - Titre du projet de rechercheContribution of the glutamine/glutamate cycle in response to STF-62247 revealed by metabolicprofiling : Correlation between the PI3K/AKT/FOXO pathway and autophagy

Poster presenter - Nom de la personne qui présentera l'afficheSarah Robichaud



Researchers involved in this project - Chercheurs participant au projetTurcotte , Sandra; Barnett, David; Robichaud, Sarah; Ouellette, Rodney.

Abstract - RésuméMetastatic Renal cell carcinomas (RCCs) pose important therapeutic challenges due to resistance toconventional chemotherapies. In more than 85% of RCCs, the von Hippel- Lindau (VHL) tumorsuppressor gene is inactivated. We have identified a small molecule, STF-62247, which isselectively cytotoxic in VHL-deficient cells. Recent studies have shown that the PI3K/AKT/FOXOpathway can modulate the glutamine/glutamate cycle and that VHL-deficient cells depend onglutamine for their survival. In this project, we aim to characterize the effect of STF-62247 on theglutamine/glutamate cycle in RCC cells. In addition, we want to investigate the PI3K/AKT/FOXOpathway and its link to glutamine levels and autophagy. Using mass spectrometry, we showed adecrease in glutamate levels in VHL-deficient cells in response to STF-62247, whereas glutaminelevels are unaffected. An increase in FOXO3 translocation following STF-62247 treatment wasshown using both nuclear fractionation and immunofluorescence. Our results also show an increasein Glutamine Synthetase levels in VHL-deficient cells due to the transcription factor’s activation.Altogether, these results could help us understand the small molecule’s mechanism of action, whichin turn could lead to the development of targeted therapies for RCCs.

Contribution du cycle glutamate/glutamine en réponse au STF-62247 révélée par une étudemétabolique : Lien entre la voie PI3K/AKT/FOXO et l'autophagie Les carcinomes rénauxmétastatiques (RCC) posent des défis thérapeutiques importants dus à l’absence de symptômesainsi que leur résistance aux chimiothérapies conventionnelles. Dans plus de 85% des patientsatteints de RCC, le gène de suppression tumoral von Hippel-Lindau (VHL) est inactivé. Nous avonsidentifié une petite molécule, le STF-62247, laquelle est spécifiquement cytotoxique dans lescellules cancéreuses rénales déficientes en VHL. Des études récentes ont démontré que la voiePI3K/AKT/FOXO est capable de moduler le cycle glutamate/glutamine et que les cellules déficientesen VHL dépendent sur la glutamine pour leur survie. Ce projet vise à caractériser l’effet duSTF-62247 sur le cycle du glutamate/glutamine dans les cellules RCC. De plus, nous voulonsinvestiguer la voie PI3K/AKT/FOXO et son lien avec les niveaux de glutamine ainsi quel’autophagie. En utilisant la spectrométrie de masse, nous avons démontré une baisse des niveauxde glutamate chez les cellules déficientes en VHL alors que les niveaux de glutamine ne sont pasaffectés. Une augmentation de la translocation de FOXO3 en réponse au traitement au STF-62247fut démontrée par fractionnement nucléaire et par immunofluorescence. Nos résultats démontrentégalement une augmentation de l’expression de la Glutamine Synthétase chez les cellulesdéficientes en VHL due à l’activation du facteur de transcription FOXO3. Ces résultats pourraient

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aider avec la compréhension du mécanisme d’action de cette petite molécule, ce qui pourrait menerau développement de thérapies ciblées pour les RCC.

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Title of the research project - Titre du projet de rechercheContribution of the glutamine/glutamate cycle in response to STF-62247 revealed by metabolicprofiling : Correlation between the PI3K/AKT/FOXO pathway and autophagy

Poster presenter - Nom de la personne qui présentera l'afficheSarah Robichaud



Researchers involved in this project - Chercheurs participant au projetTurcotte , Sandra; Barnett, David; Robichaud, Sarah; Ouellette, Rodney.

Abstract - RésuméMetastatic Renal cell carcinomas (RCCs) pose important therapeutic challenges due to resistance toconventional chemotherapies. In more than 85% of RCCs, the von Hippel- Lindau (VHL) tumorsuppressor gene is inactivated. We have identified a small molecule, STF-62247, which isselectively cytotoxic in VHL-deficient cells. Recent studies have shown that the PI3K/AKT/FOXOpathway can modulate the glutamine/glutamate cycle and that VHL-deficient cells depend onglutamine for their survival. In this project, we aim to characterize the effect of STF-62247 on theglutamine/glutamate cycle in RCC cells. In addition, we want to investigate the PI3K/AKT/FOXOpathway and its link to glutamine levels and autophagy. Using mass spectrometry, we showed adecrease in glutamate levels in VHL-deficient cells in response to STF-62247, whereas glutaminelevels are unaffected. An increase in FOXO3 translocation following STF-62247 treatment wasshown using both nuclear fractionation and immunofluorescence. Our results also show an increasein Glutamine Synthetase levels in VHL-deficient cells due to the transcription factor’s activation.Altogether, these results could help us understand the small molecule’s mechanism of action, whichin turn could lead to the development of targeted therapies for RCCs.

Contribution du cycle glutamate/glutamine en réponse au STF-62247 révélée par une étudemétabolique : Lien entre la voie PI3K/AKT/FOXO et l'autophagie Les carcinomes rénauxmétastatiques (RCC) posent des défis thérapeutiques importants dus à l’absence de symptômesainsi que leur résistance aux chimiothérapies conventionnelles. Dans plus de 85% des patientsatteints de RCC, le gène de suppression tumoral von Hippel-Lindau (VHL) est inactivé. Nous avonsidentifié une petite molécule, le STF-62247, laquelle est spécifiquement cytotoxique dans lescellules cancéreuses rénales déficientes en VHL. Des études récentes ont démontré que la voiePI3K/AKT/FOXO est capable de moduler le cycle glutamate/glutamine et que les cellules déficientesen VHL dépendent sur la glutamine pour leur survie. Ce projet vise à caractériser l’effet duSTF-62247 sur le cycle du glutamate/glutamine dans les cellules RCC. De plus, nous voulonsinvestiguer la voie PI3K/AKT/FOXO et son lien avec les niveaux de glutamine ainsi quel’autophagie. En utilisant la spectrométrie de masse, nous avons démontré une baisse des niveauxde glutamate chez les cellules déficientes en VHL alors que les niveaux de glutamine ne sont pasaffectés. Une augmentation de la translocation de FOXO3 en réponse au traitement au STF-62247fut démontrée par fractionnement nucléaire et par immunofluorescence. Nos résultats démontrentégalement une augmentation de l’expression de la Glutamine Synthétase chez les cellulesdéficientes en VHL due à l’activation du facteur de transcription FOXO3. Ces résultats pourraient

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Title of the research project - Titre du projet de rechercheAssessing the risk of Lyme borreliosis in New Brunswick

Poster presenter - Nom de la personne qui présentera l'afficheJulie Lewis



Researchers involved in this project - Chercheurs participant au projetLewis, Julie; Patterson, Jamie; Harris, Kami; Filiaggi, Corey; Bjurman, Natalie; Lloyd, Vett.

Abstract - RésuméBackground: Lyme borreliosis (LB), commonly known as Lyme disease, is an emerging infectiousdisease in New Brunswick. The primary causative agent, Borrelia burgdorferi, is transmitted by ticks,in New Brunswick principally Ixodes scapularis. Goal: To monitor tick abundance and infection ratein different regions of the province, which is important in targeting public health messages onprevention of tick borne diseases. Methods: Ticks were collected using both passive and activesurveillance methods. Ticks were received from veterinary clinics and the general public as well ascollected from communities across the province. Tick infection status is determined by DNAextraction and nested PCR for two Borrelia genes, OspA and FlaB. Additional information on tickinfection frequency can be inferred from canine seropositivity rates. Results: In 2013, we received436 ticks and, in 2014, we have received 350 ticks to date. Analysis of the rate of infection and thelocations in which different tick life stages are found suggests that the southern third of the province,coinciding with the major population centers, and the coastal region are higher risk areas. Significance: This data shows that infected ticks can be found throughout the province. The dataalso suggests that tick populations are established throughout much of the province. As tickpopulations become established, this will increase the number of ticks and the risk of contracting tickborne illnesses.

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Title of the research project - Titre du projet de rechercheCharacterization of the Pax5 interactome: PARP-1 can influence B-cell-specific gene expression

Poster presenter - Nom de la personne qui présentera l'afficheCharles E. Bullerwell


Education LevelPost-Doctoral Fellows

Researchers involved in this project - Chercheurs participant au projetBullerwell, Charles; Crapoulet, Nicolas; Richard, Rémi; Barnett, David; Joy, Andrew; Fournier,Sébastien; Daigle, Mélissa; Ouellette, Rodney.

Abstract - RésuméPax5 is a commonly mutated transcription factor in B cell lymphoma with pivotal roles in Blymphocyte differentiation. We demonstrate that Pax5 expressed exogenously in HEK293T cellsinteracts with the DNA repair protein PARP-1. This interaction is also detected when examiningendogenous Pax5 in a human B cell line as well as in a GST pulldown assay. In addition to itswell-characterized roles in DNA repair, PARP-1 is also known to influence transcription regulation ina variety of contexts. PARP-1 has previously been shown to interact with the closely-related Pax8and influence the ability of Pax8 to activate transcription. Similarly, we demonstrate functionalinteractions between Parp-1 and Pax5. These two proteins are able to synergistically increasetranscription in a reporter assay, and silencing of PARP-1 is able to decrease mRNA levels ofendogenous CD19 in a B cell line. PARP-1 inhibitor treatments do not suggest a role for parylationby PARP-1 in these functional interactions. Finally, mass spectrometric analysis of proteinsco-immunoprecipitating with Pax5 both in HEK293T cells and in several B cell lines identify proteinsknown to be associated with Pax5 as well as with PARP-1. This suggests the recruitment ofPARP-1-containing complexes to Pax5. These results expand our understanding of PARP-1’s rolesin transcription, increase our understanding of complexes that regulate Pax5 transcription regulation,and suggest novel roles for both Pax5 and PARP-1 in B cell development.

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Title of the research project - Titre du projet de rechercheSCD-1 activation in prostate cancer cells by androgens

Poster presenter - Nom de la personne qui présentera l'afficheSabrina Duguay



Researchers involved in this project - Chercheurs participant au projetSurette, Marc.

Abstract - RésuméProstate cancer is the most commonly diagnosed cancer among Canadian men. Unfortunately,treatment of prostate cancer remains a clinical challenge and new therapeutic targets are sought. Ascancer cells proliferate, they require unsaturated fatty acids for membrane biosynthesis. In humans,stearoyl-CoA desaturase-1 (SCD-1) is the main enzyme responsible for monounsaturated fatty acids(MUFA) synthesis. We previously observed that SCD-1 is overexpressed in hormone-dependentbreast cancer cells treated with estradiol, and cell proliferation is significantly inhibited when SCD-1is inhibited. Like breast cancer, prostate cancer can also be hormone-dependent. The goal of thisresearch project is to determine whether androgens induce SCD-1 expression in hormone sensitiveLNCaP prostate cancer cells. LNCaP cells were incubated in steroid-free medium for 3 days to slowcell proliferation, and then incubated in medium supplemented or not with 5α-dihydrotestosterone(5α-DHT). Cellular fatty acids were measured by gas chromatography and SCD-1 expression byqPCR and immunoblot analysis. The incubation of cells with 5α-DHT resulted in a significant changein the cellular fatty acid profiles, including an increase in the cellular MUFA content. This change incellular fatty acid profiles was accompanied by increased expression of SCD-1 compared to cellsincubated in the absence of 5α-DHT. These results suggest that SCD-1 expression is required forthe hormone-dependent progression of prostate cancer. Further experiments investigating the roleof SCD-1 in prostate cancer cell survival and proliferation are underway. Overall, this research maylead to the identification of new targets for the treatments of prostate cancer.

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Title of the research project - Titre du projet de rechercheL'entrainement par exercice supramaximal répété améliore la contribution lipidique à la fournitureénergétique lors d'un test incrémental chez des adultes obèses

Poster presenter - Nom de la personne qui présentera l'afficheAnne Paulin



Researchers involved in this project - Chercheurs participant au projetJabbour, Georges.

Abstract - RésuméL’entrainement par exercice intermittent à haute intensité constitue une nouvelle stratégieinterventionnelle auprès des personnes obèses. À cet effet, le modèle souvent utilisé est le testWingate, dont l’exigence de l’effort (30 s d’exercice supramaximal) limite le taux de participation.C’est pourquoi notre laboratoire a développé un modèle d’exercice à haute intensité d’exécutionbrève. But : Évaluer l’effet de 6 semaines d’entrainement par exercice à haute intensité (EEHI) (6 x6 s d’effort supramaximal entrecoupés de 2 min de récupération) sur l’oxydation lipidique lors d’unexercice incrémental chez des adultes obèses. Méthode : 24 adultes obèses (IMC ± 33 kg.m- 2)furent divisés en deux groupes : non entrainé (NE) et EEHI. Les % de la contribution lipidique à lafourniture énergétique via l’oxydation (% OL) aux paliers 25, 50 et 75 Watts ont été mesurés chezles 2 groupes, avant et après l’EEHI. Résultats : Avant la période d’entrainement, les % OL aurepos et à 25, 50 et 75 Watts ne différaient pas statistiquement entre les groupes. Aprèsl’entrainement, ces % augmentèrent chez le groupe EEHI (+20% à 25W, +21% à 50W, +14% à75W; p<0.01) et étaient significativement plus élevés chez ce dernier que chez le groupe NE.Conclusion : Chez des adultes obèses, bien que leur % de masse maigre ne soit pas modifié, 6semaines d’EEHI d’exécution brève augmentent la contribution lipidique lors d’un effort incrémental.Ce modèle d’EEHI, plus tolérable que le Wingate, requiert une attention particulière dans la prise encharge de l’obésité.

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Title of the research project - Titre du projet de rechercheChildhood obesity affects balance control and aiming performance

Poster presenter - Nom de la personne qui présentera l'afficheGrant Handrigan



Researchers involved in this project - Chercheurs participant au projetHandrigan, Grant; Boucher, François; Mackrous, Isabelle; Hue, Olivier.

Abstract - RésuméObesity reduces the efficiency of balance and movement control mechanisms. However, the effectsof obesity on a functional motor task and balance control in standing and seated position have notbeen closely quantified among children. The aim of this study is to examine the effects of obesity onthe execution of aiming tasks performed in standing and seated conditions in children. Twelvehealthy weight children and eleven obese children aged between 8 and 11 years pointed to a targetin standing and seated position. The difficulty of the aiming task was varied by using 2 target sizes.Hand movement time (MT) and its phases were measured to quantify the aiming task. Mean speedof the center of pressure displacement (COP speed) was calculated to assess balance controlduring the movement. Obese children had significantly higher MTs compared to healthy-weightchildren in seated and standing conditions explained by greater durations of deceleration phasewhen aiming. Concerning the COP speed during the movement, obese children showed significantlyhigher values when standing compared to healthy-weight children. This was also observed in theseated position. In conclusion, obesity adds a balance constraint during an aiming task in bothseated and standing conditions and requires obese children to take more time to correct theirmovements due to a greater balance instability of the body when pointing to a target with theupper-limb.

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Title of the research project - Titre du projet de rechercheThe Role of Heme Regulatory Enzymes in Myocytes under Stress

Poster presenter - Nom de la personne qui présentera l'afficheAshley Eadie



Researchers involved in this project - Chercheurs participant au projetBrunt, Keith.

Abstract - RésuméBackground: Heme oxygenase-1, the stress response enzyme responsible for heme catabolism,has been extensively studied as a potential therapeutic in the management of pre-clinicalcardiovascular disease, however recent high-profile studies have also demonstrated that itsupregulation drives symptoms of early onset Type II diabetes, a potent risk factor for developingcardiovascular disease. These findings highlight the requirement for a more in depth understandingof how heme metabolism is regulated before it can be targeted as a therapeutic agent. Objective:To investigate how heme is regulated in cardiomyocytes under stress states similar to thoseexpressed in cardiovascular patients. Methods and Results: Our results indicate that hemeregulatory protein levels, including ALAS2, vary significantly in mice subjected to myocardialinfarction. Heme content and heme regulatory proteins, size and survival of H9C2 ratcardiomyogenic cells under normal and stress states, such as hypoxia, inflammatory and oxidativemodels show variable changes in both heme synthesis and catabolism enzymes. Conclusion: Agreater homeostatic understanding of molecular targets used for therapy is warranted prior toengaging them in advanced pre-clinical or clinical models of disease.

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Title of the research project - Titre du projet de rechercheHNRNP H IS A NOVEL STRESS GRANULE-ASSOCIATED PROTEIN AND ASSISTS INCELLULAR STRESS RECOVERY

Poster presenter - Nom de la personne qui présentera l'afficheMichael Wall


Education LevelPost-Doctoral Fellows

Researchers involved in this project - Chercheurs participant au projetWall, Michael; Wong, Florence; Lewis, Stephen.

Abstract - RésuméIn human cells, signaling pathways are activated in response to a variety of intra- and extracellularstresses. These molecular signals are integrated over time and dictate whether a cell will undergoprogrammed cell death (also known as apoptosis) or begin recovery from the stress. An importantaspect of the stress response is the formation of cytoplasmic stress granules. These structures formquickly in response to a variety of stresses and are composed of stalled mRNA, ribosomal subunitsand a variety of RNA-binding proteins. Many RNA-binding proteins interact transiently with stressgranules to direct specific mRNAs for protection or degradation during stress conditions.Interference with stress granule formation or the absence of associated interacting proteins cannegatively impact the cellular response to stress and cellular recovery. The heterogeneous nuclearribonucleoprotein (hnRNP) family of RNA-binding proteins contains several members that are knownto transiently interact with stress granules during cellular stress conditions, most notably hnRNP A1.Our present research shows that another hnRNP family member, hnRNP H, is a stressgranule-associated protein that plays a role in the stress response. Reduction of hnRNP H proteinabundance reduces the ability of HeLa cells to recover from osmotic stress. In addition, we identifiedthe RNA recognition motif 3 (RRM3) as a critical domain for efficient stress granule association ofhnRNP H. Possible mechanisms that regulate hnRNP H-stress granule interactions and theirsignificance to cancer are discussed.

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Title of the research project - Titre du projet de rechercheDietary intake of DHA and EPA in a group of pregnant women in the Moncton area

Poster presenter - Nom de la personne qui présentera l'afficheNicole Arsenault Bishop



Researchers involved in this project - Chercheurs participant au projetArsenault Bishop, Nicole; LeBlanc, Caroline P..

Abstract - RésuméDocosahexaenoic acid (DHA) is found in abundance in the gray matter of the brain and retinalphotoreceptors and its accumulation in the fetus occurs primarily during the last trimester ofgestation. Since the synthesis of DHA and eicosapentaenoic acid (EPA) is limited by the placentaand the fetus, the maternal diet becomes crucial. To date, only linoleic acid and alpha-linolenic acidhave Dietary Reference Intakes established. The Food and Agriculture Organization (FAO) / WorldHealth Organization (WHO) recommend that pregnant women consume at least 200 mg/d DHA and300 mg/d DHA and EPA combined and Health Canada recommends consuming two Canada FoodGuide (CFG) servings of fish weekly. The purpose of this study is to compare the intake of DHA andEPA pregnant women in the Moncton area to the recommendations suggested by the FAO / WHO /CFG and to identify barriers that play a role in the consumption of foods rich in DHA and EPA.Pregnant women (n = 27) at 30 weeks of gestation completed an interview with a dietitian using asemi-quantitative food frequency questionnaire. Preliminary results show that women consume 2 ±0.55 CFG servings of fish/week and have daily intakes of 218.1 ± 14.9 mg DHA and 89.4 ± 6.7 mgEPA. Common barriers shown are the lack of fish and seafood cooking ideas (58.33%) and the highcost of fish (41.67%). In this small group of socio economically privileged mothers in the Monctonarea, recommendations of FAO / WHO and CFG are attained.

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Title of the research project - Titre du projet de rechercheAmyotrophic Lateral Sclerosis Incidence and Cluster Identification in New Brunswick, Canada, overa 10-Year Period

Poster presenter - Nom de la personne qui présentera l'afficheJonathan Jean



Researchers involved in this project - Chercheurs participant au projetJean, Jonathan; O'Connell, Colleen; McCullum, Shane.

Abstract - RésuméAmyotrophic lateral sclerosis (ALS) is a rare and devastating neurodegenerative diseasecharacterized by degeneration of the motor neurons located in the primary motor cortex, spinal cordand brainstem. The exact etiology of the disease is unknown. Currently there is no cure for thedisease and patients diagnosed typically die within three to five years after diagnosis. To date,there are no studies on ALS in our New Brunswick (NB) population. The purpose of this study is toperform a retrospective epidemiology study of ALS in New Brunswick over the last 10 years, andidentify any unique patterns or clusters that could contribute to our knowledge about the disease.We have created a database of persons diagnosed with ALS between 2003-2013, from records ofthe Stan Cassidy Centre for Rehabilitation (SCCR). Data includes date of symptom onset, date ofdiagnosis, age, and pattern of disease onset. Cluster analysis has been performed, which isimportant in studying this disease for assessment of genetic and environmental factors contributingto the disease. From statistical analysis of this database, the 10-year prevalence was calculated tobe 22.8 per 100,000. This highest yearly incidence was found to be 3.73 per 100,000 for 2012.These results are an underrepresentation of the actual abundance of ALS in the province, butappear to indicate a higher than expected incidence, with early evidence for possible clusters. Previous international epidemiological studies suggest a yearly incidence of 1-2 cases per 100,000and a prevalence of 6-10 per 100,000.

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Title of the research project - Titre du projet de rechercheDieldrin augments mTOR signaling and inhibits autophagy in the adult zebrafish heart (Danio rerio)

Poster presenter - Nom de la personne qui présentera l'afficheLogan Slade



Researchers involved in this project - Chercheurs participant au projetSlade, Logan; Cowie, Andrew ; Trivedi , Purvi ; Martyniuk , Christopher ; Kienesberger, Petra ;Pulinilkunnil, Thomas .

Abstract - RésuméBackground: Dieldrin, a pesticide and endocrine disruptor, continues to persist in the environmentdespite being discontinued. Dieldrin is associated with increased risks of neurodegenerative diseaseand breast cancer. Research focusing on the transcriptomic response to dieldrin exposure hasidentified genes that modulate cell survival, and inflammation in the neuroendocrine system. Despitethese findings, the effects of dieldrin in the heart remains unknown. Aim: To examine the effects ofdieldrin on cell survival and growth signaling pathways in the zebrafish heart. Methods and Results:Adult zebrafish (Danio rerio) were treated with dieldrin (1.988 μg per gram of feed) for 21 days andhearts isolated for biochemical analysis. Immunoblot analysis demonstrated that there was anincrease in Akt phosphorylation at Serine 473 in the hearts of dieldrin treated zebrafish compared tocontrols. Furthermore, this increase in Akt stimulation corresponded with increased downstreamphosphorylation of its targets mTOR (Ser2448) and ribosomal s6 kinase (Thr389), inducers ofprotein synthesis and cell growth. Increased protein synthesis is concomitantly associated withinhibition of autophagic protein degradation as evidenced by downregulation of cathepsin D, alysosomal protease. Our data are in agreement with previous reports highlighting the estrogenic roleof dieldrin and being causative for Akt activation in vitro and in vivo. These data suggest that dieldrininterferes with the ability of the cardiomyocyte to undergo physiological autophagy byhyperactivating Akt-mTOR axis in the zebrafish heart. Significance: This research highlights theimportance of zebrafish as a model vertebrate system to study molecular mechanisms of pesticidecardiotoxicity.

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Title of the research project - Titre du projet de rechercheA One Year Pilot Study of Circuit Class Outcomes in Outpatient Neurological Population

Poster presenter - Nom de la personne qui présentera l'afficheShane McCullum



Researchers involved in this project - Chercheurs participant au projetMcCullum, Shane; Leroux, Meghan; Jones, Melony; de Passille, Erica.

Abstract - RésuméCircuit Class therapy has been widely studied, and has been shown to be an effective means ofPhysiotherapy treatment for patients who are post-stroke. There is a significant amount ofheterogeneity within neurological rehabilitation, so it is difficult to extrapolate results from onediagnosis to others. The purpose of this pilot project is to quantify the effectiveness of Circuit ClassPhysiotherapy in the outpatient neurological population. The Circuit Class was held twice weeklyand each group of patients participates for 8 weeks. Each Circuit Class session was divided into 4stations and took a total of 60 minutes. After a 5 minute warm-up, patients were divided into groupsand rotated through stations focusing on cardiovascular endurance, leg strength, arm strength andpostural balance. A 5 minute cool-down concluded each session. Outcome measures wereassessed at baseline, upon completion of the 8 week class, and 12 weeks after completion of theclass. A total of 20 subjects were enrolled in the study, with 17 who completed all 3 assessmentperiods for the study. Statistically significant improvements were found for all physical outcomemeasures, with no significant decline at the 12 week post-class period. The next step in this study islooking at physical outcomes of a community circuit class in partnership with the local YMCA(anticipated start date of fall 2015).

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Title of the research project - Titre du projet de rechercheA new surgical approach that use polypropylene tapes for gynecological prolapse repair

Poster presenter - Nom de la personne qui présentera l'afficheDr. Alfred Robichaud

First author - Premier auteurNon


Researchers involved in this project - Chercheurs participant au projetRobichaud, Alfred; Bélanger, Mathieu; Poirier, Martine; Cloutier, Frank.

Abstract - RésuméIntroduction: Each year, approximately 300 000 women undergo surgery for pelvic organ prolapse(POP) in North America. Different surgical procedures have been developed to treat POP but have arecurrence rate of 29 to 40%. Synthetic mesh kits have been developed and reduced the recurrencerate to a range of 4-13%. However, mesh-related complications, including dyspareunia, pain andmesh erosion, reduced quality of life, have been reported. Objective: To determine the efficacy of anew approach using a vaginally-installed polypropylene tape (VPPT) to treat prolapse. Methods:Patients with a vaginal prolapse received the procedure and were included in the study. Primaryoutcome was objectively assessed recurrence of prolapse. Secondary outcomes measures wereoperative complications, adverse events, and self-reported urinary, digestive, and sexual functionsassessed using validated questionnaires. Results: 75 patients received the procedure and werefollowed-up for an average (standard deviation) of 27 (18.7) months. Only 2 (2.6%) womenexperienced a recurrence of their vaginal prolapse and this occurred at 6 months of follow-up, givinga cumulative incidence rate of 0.004 recurrences per patient-month. Only one complication wasnoted and was related to a secondary procedure performed at the time of POP surgery. Conclusion:The new VPPT surgical procedure is safe and has a very high level of efficacy to treat POP.

Introduction: Chaque année, plus de 300 000 femmes subissent une chirurgie pour corriger unprolapsus d’un organe pelvien (POP). Différentes procédures chirurgicales ont été développéespour corriger un POP mais le taux de récurrence se situe entre 29% et 40%. L’élaboration desystèmes utilisant un grand treillis de polypropylène a permis de réduire le taux de récurrence qui sesitue maintenant entre 4% et 13%. Cependant, des complications reliées au treillis incluant sonérosion et des effets indésirables comme la dyspareunie, de la douleur ainsi qu’une diminution dela qualité de vie ont été rapportés. Objectifs : Déterminer l’efficacité d’une nouvelle approchechirurgicale utilisant de petites bandelettes de polypropylène. Méthode : Des patientes avec unprolapsus vaginal ont été recrutées dans cette étude. La participation a nécessité 8 visites de suiviqui se sont échelonnées sur une période de 5 ans. À chaque visite, la patiente devait répondre àdes questions concernant les symptômes reliés à la douleur, le fonctionnement urinaire, intestinal etsexuel ainsi que des questions reliées à la qualité de vie. De plus, un examen physique a étéeffectué. Résultats : L’intervention chirurgicale a été réalisée sur 75 patientes qui ont été suivispendant une moyenne de 27 ± 19.7 mois. Seulement 2 (2.6%) femmes ont développé à nouveau unprolapsus vaginal 6 mois après la chirurgie. L’incidence cumulative du taux de récurrence estseulement de 0.004 par patiente par mois. Conclusion: Cette nouvelle approche est sécuritaire avecun excellent niveau d’efficacité.

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Title of the research project - Titre du projet de rechercheExpressions of Sox5 and Sox13 transcription factors are increased in testicular Leydig cells of miceduring postnatal development/ Les expressions des facteurs de transcription Sox5 et Sox13augmentent dans les cellules de Leydig du testicule murin lors du développement postnatal

Poster presenter - Nom de la personne qui présentera l'afficheMikella Daigle



Researchers involved in this project - Chercheurs participant au projetMartin, Luc J..

Abstract - RésuméSox members are expressed in numerous tissues and regulate a variety of developmental stages. Inpostnatal testes, members of this family have been characterized. However, expressions ofmembers of this family of transcription factors have never specifically been shown in adult Leydigcells. These cells supply testosterone necessary for the onset and maintenance ofspermatogenesis. The objectives of this research are to locate and determine the expressionprofiles of two SoxD members, Sox5 and Sox13, in postnatal mice testis at different developmentalstages, and to identify their expression in mice Leydig cell cultures. mRNA and proteinquantifications of Sox5 and Sox13 from whole mice testis at three different ages (P33, P56 andP120) as well as in MA-10 cell cultures were done using qPCR and Western Blots, respectively.Immunofluorescence was used to locate Sox5 and Sox13 protein expressions from whole micetestis at the same three ages. Sox5 and Sox13 mRNAs and proteins have been characterized inMA-10 as well as from whole testis. Using immunofluorescence of mice testis, Sox5 and Sox13expressions were located in Leydig cells and increased with age. To our knowledge, this is the firststudy showing the presence of Sox5 and Sox13 transcription factors in adult Leydig cells. Theseproteins may regulate multiple functions of these cells, such as steroidogenesis important forpuberty and spermatogenesis. However, their role and mechanisms of actions in postnatal testesremain to be investigated.

Les membres de la famille Sox sont exprimés dans divers tissus et ils régulent une variété deprocessus de développement. Certains membres de cette famille ont été caractérisés dans lestesticules postnataux, mais jamais spécifiquement dans les cellules de Leydig adultes. Ces cellulesfournissent la testostérone nécessaire à la puberté et à la spermatogenèse. Les objectifs de cetterecherche sont d’identifier et localiser les profils d’expression de Sox5 et Sox13 dans le testiculemurin à différents stades postnataux et d’identifier leurs expressions au niveau de culturescellulaires de Leydig. Les quantifications des ARNm et des protéines Sox5 et Sox13 à partir de troisâges de testicules entiers de souris (P33, P56 et P120) ainsi que de cellules MA-10 ont étéeffectuées par qPCR et Western Blot, respectivement. L’immunofluorescence a localisé lesexpressions protéiques de Sox5 et Sox13 dans les testicules entiers aux mêmes âges. L’ARNm etles protéines de Sox5 et Sox13 ont été caractérisées dans les cellules MA-10 et dans les testiculesentiers. À partir d’immunofluorescence, Sox5 et Sox13 ont été localisés au niveau des cellules deLeydig murines et leurs expressions augmentaient avec l’âge. Cette étude est la première à

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démontrer la présence des facteurs de transcription Sox5 et Sox13 dans les cellules de Leydigadultes. Ces protéines pourraient réguler plusieurs processus, comme la stéroïdogenèse.Cependant, leurs rôles dans le testicule postnatal demeurent à être étudiés.

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Title of the research project - Titre du projet de rechercheSynthetic Lethality Screen of Tumor Suppressor Pathways for Therapeutic Compound Development

Poster presenter - Nom de la personne qui présentera l'affichePierre Deprez



Researchers involved in this project - Chercheurs participant au projetDeprez, Pierre.

Abstract - RésuméCancer is the leading cause of premature death in Canada as well as Atlantic Canada.Notwithstanding certain successes, there is a great need for improved anti-cancer therapies,especially for tumors of lung, gastro-intestinal tract, breast and kidney origin that make up themajority of cancer diagnosed in the world. Most chemotherapy approaches still target multiplyingcells in general and are not selective for cancer cells. Such non-selectivity leads to the toxicside-effects experienced by cancer patients, since rapidly dividing normal cells in the hematopoieticand digestive systems are also targeted. In order to identify novel therapeutic targets andcompounds we focus on tumor-specific alterations that can induce cancer cell death by blocking orinhibiting a pathway that acts in parallel to one that is only altered in the tumor. We are currentlyusing this approach on the most common cancer by performing large scale genomic deletions usingthe CRISPR-Cas9 system. We hereby show NGS results that feature potential therapeutic targetsthat may be used to design compounds for the treatment of certain cancers.

Le cancer est la cause principale de décès prématuré au Canada ainsi que dans le CanadaAtlantique. Alors que montrant des résultats, il est nécessaire d’améliorer les thérapiesanti-cancéreuses actuelles, principalement dans le cadre des cancers du poumon,gastro-intestinaux, du sein et des reins, qui représentent la majorité des cancers diagnostiqués dansle monde. La plupart des traitements de chimiothérapie ciblent toutes les cellules qui se multiplientrapidement et pas seulement les cellules cancéreuses. Un tel manque de sélection entrainent deseffets secondaires toxiques chez les patients puisque les cellules des systèmes sanguin et digestifqui se divisent de façon rapide sont aussi ciblées. Ayant pour objectif la découverte de nouveauxcomposés ainsi que de nouvelles cibles thérapeutiques, nous nous concentrons sur les altérationsspécifiques aux tumeurs qui peuvent induire la mort des cellules cancéreuses en bloquant ouinhibant des voies moléculaires qui fonctionnent parallèlement à d’autres qui sont altérées dans lestumeurs. Actuellement, nous utilisons cette approche sur les cancers les plus communs en réalisantdes délétions génomiques à grande échelle par l’utilisation du système CRISPR-Cas9. Nousprésentons ici les résultats du NGS qui mettent en évidence les cibles thérapeutiques potentiellesqui pourront servir à l’élaboration de nouveaux composés pour le traitement de certains cancers.

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Title of the research project - Titre du projet de rechercheInvestigation of the mechanisms by which alternatively-spliced 5-lipoxygenase isoforms inhibitleukotriene biosynthesis

Poster presenter - Nom de la personne qui présentera l'afficheEric Allain



Researchers involved in this project - Chercheurs participant au projetSurette, Marc; Allain, Eric; Boudreau, Luc; Flamand, Nicolas.

Abstract - RésuméBACKGROUND: 5-lipoxygenase (5-LO) catalyzes the biosynthesis of leukotrienes (LTs), mediatorsof inflammation associated with inflammatory diseases. Understanding mechanisms of 5-LOregulation may identify new approaches to control LT biosynthesis. We previously identified severalsplice variants of 5-LO in human leukocytes (Boudreau et al, FASEB J, 2011). The most commonalternative isoform is ∆13 whose transcript lacks exon 13 and which lacks 5-LO catalytic activity. Wepreviously reported that ∆13 inhibits LT biosynthesis when co-expressed with full length active 5-LO.OBJECTIVE: To determine whether the mechanism by which ∆13 inhibits LT biosynthesis is byblocking the interaction of full length active 5-LO with companion proteins FLAP or coactosin-likeprotein (CLP). METHODS and RESULTS HEK293 cells were co-transfected to express the active5-LO in with or without the ∆13 isoform, in the presence or absence of FLAP. Cells were stimulatedwith calcium ionophore and 5-LO products were measured by HPLC. ∆13 inhibited LT biosynthesisin a dose dependent manner whether the cells co-expressed FLAP or not. When active5-LO-expressing cells were transfected with the W102A mutant of Δ13 that cannot interact withCLP, LT biosynthesis was still inhibited. These results suggest that Δ13-FLAP and Δ13-CLPinteractions are not involved in the inhibitory effect. Interestingly, the full-length 5-LO and ∆13showed different subcellular localization measured by confocal microscopy and differentphosphorylation patterns assessed by immunoblots. CONCLUSIONS. These results show that the5-LO isoforms occupy different subcellular compartments and that ∆13 appears to inhibit LTbiosynthesis by a mechanism not involving known partner proteins.

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Title of the research project - Titre du projet de rechercheEffet de l'antagonisme des récepteurs nicotiniques sur les monocytes

Poster presenter - Nom de la personne qui présentera l'affichePatrick Roy



Researchers involved in this project - Chercheurs participant au projetRoy, Patrick; Simard, Alain.

Abstract - RésuméNotre système immunitaire est responsable de la réparation de tissus et de la protection contre lespathogènes, deux fonctions bénéfiques pour le maintien de la santé. Cependant, il peut aussi yavoir des rôles néfastes, tel qu’observé chez les gens atteint de Sclérose en plaques, où la myélinese fait attaquer par nos propres cellules immunitaires. Plusieurs études ont démontrées que lesystème nerveux peut influencer notre système immunitaire et que la nicotine, un ligand auxrécepteurs nicotiniques à l’acétylcholine (nAChRs), a des effets anti-inflammatoires et protègecontre la maladie chez un modèle animal de la Sclérose en Plaques. Même si la nicotine est mieuxconnue comme étant un agoniste au nAChRs, une présence chronique de fortes doses de nicotine(˃10μ M) désensibilise les récepteurs, agissant donc comme un antagoniste. L’objectif de cetteétude était de tester si les effets anti-inflammatoires de la nicotine au niveau des monocytes est dûà l’activation ou à l’inhibition des nAChRs. Nous avons étudié l’effet de la mécamylamine, unantagoniste des nAChRs, sur la prolifération, la différenciation et la survie des monocytes dans unmodèle de culture primaire. Nous avons trouvé que la mécamylamine inhibe la prolifération desmonocytes et diminue la viabilité de ces cellules. De plus, cette molécule réduit l’expression desmarqueurs pro-inflammatoires chez les monocytes survivants. Ces résultats suggèrent donc quel’antagonisme ou la désensibilisation des nAChRs est le mécanisme par lequel le systèmecholinergique contrôle l’inflammation. Nos études pourraient mener à la création de nouveauxtraitements contre les maladies inflammatoires.

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Title of the research project - Titre du projet de rechercheRessemblance entre les pratiques de divers types d’activité physique chez des enfants en 5e et 6eannée et leurs parents

Poster presenter - Nom de la personne qui présentera l'afficheJason MacKenzie



Researchers involved in this project - Chercheurs participant au projetMacKenzie, Jason; Brunet, Jennifer; Wing, Erin, K; Bélanger, Mathieu.

Abstract - RésuméPhysical activity (PA) is associated with better physical, psychological and social health outcomes inyouth. Only 7% of Canadian children meet PA guidelines. Although parents can influence theirchild’s PA through role modeling, little is known about the extent to which parents and youthresemble each other in regards to participation in specific types of PA. We examined theassociations between parents’ and youth’s participation in racket sports, rhythmic, movement andgymnastic activities, fitness activities, physical leisure pursuits, active recreation, team sports andgames, and daily living activities. 214 families were included. Youth were grade 5 and 6 studentsfrom 17 schools in NB, Canada. Data were collected as part of the MATCH project withself-administered questionnaires for youth and telephone questionnaires for adults. Odds ratios(OR) and positive and negative predictive values (PPV, NPV) were calculated. Parents and youth’sparticipation in racquet sports were significantly associated (OR = 3.80). There were no significantassociations for the other types of physical activities (p < .05). The probability that parents and youthparticipated in the same types of activities was moderate to high (PPV: 0.55-0.86), whereas theprobability that neither parents nor youth participated in a particular type of activity was low tomoderate (NPV: 0.08-0.58). This study suggests that some but not all types of PA practiced byparents and their children are associated with each other.

L’activité physique (AP) est associée une meilleure santé physique, psychologique et sociale chezles enfants. Seulement 7% des jeunes canadiens atteignent les normes d’AP canadiennes. Lesparents peuvent influencer la pratique d’AP de leurs jeunes en agissant comme modèle de rôlemais, peu est connu sur la ressemblance pour des types spécifiques d’AP. Cette étude examine desliens entre la pratique des sports de raquette, les activités rythmiques et gymnastiques, les activitésde conditionnement, les activités physiques de plaisir, des activités récréatives, le sport d’équipe etles activités quotidiennes. 214 familles ont été inclues. Les jeunes ont été recrutés à partir de la 5eet 6e année de 17 écoles du NB, Canada. Les données ont été ramassées dans le cadre du projetMATCH avec des questionnaires auto-administrés pour les jeunes et des questionnairestéléphoniques pour les parents. Des rapports de cotes (OR) et des valeurs prédictives positives etnégatives (VPP, VPN) ont été calculés. La pratique de sports de raquettes est significativementassociée entre les parents et les enfants (OR = 3,80). Aucune autre relation significative a ététrouvé (p < 0,05). La probabilité que les parents et leur enfant pratique le même type activité étaitélevé (VPP : 0,55–0,86) tandis que la probabilité que ni le parent ni l’enfant pratique un même typed’AP était bas (VPN : 0,08-0,58). Cette étude suggère que certains types d’AP pratiqués par des

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Title of the research project - Titre du projet de rechercheRessemblance entre les pratiques de divers types d’activité physique chez des enfants en 5e et 6eannée et leurs parents

Poster presenter - Nom de la personne qui présentera l'afficheJason MacKenzie



Researchers involved in this project - Chercheurs participant au projetMacKenzie, Jason; Brunet, Jennifer; Wing, Erin, K; Bélanger, Mathieu.

Abstract - RésuméPhysical activity (PA) is associated with better physical, psychological and social health outcomes inyouth. Only 7% of Canadian children meet PA guidelines. Although parents can influence theirchild’s PA through role modeling, little is known about the extent to which parents and youthresemble each other in regards to participation in specific types of PA. We examined theassociations between parents’ and youth’s participation in racket sports, rhythmic, movement andgymnastic activities, fitness activities, physical leisure pursuits, active recreation, team sports andgames, and daily living activities. 214 families were included. Youth were grade 5 and 6 studentsfrom 17 schools in NB, Canada. Data were collected as part of the MATCH project withself-administered questionnaires for youth and telephone questionnaires for adults. Odds ratios(OR) and positive and negative predictive values (PPV, NPV) were calculated. Parents and youth’sparticipation in racquet sports were significantly associated (OR = 3.80). There were no significantassociations for the other types of physical activities (p < .05). The probability that parents and youthparticipated in the same types of activities was moderate to high (PPV: 0.55-0.86), whereas theprobability that neither parents nor youth participated in a particular type of activity was low tomoderate (NPV: 0.08-0.58). This study suggests that some but not all types of PA practiced byparents and their children are associated with each other.

L’activité physique (AP) est associée une meilleure santé physique, psychologique et sociale chezles enfants. Seulement 7% des jeunes canadiens atteignent les normes d’AP canadiennes. Lesparents peuvent influencer la pratique d’AP de leurs jeunes en agissant comme modèle de rôlemais, peu est connu sur la ressemblance pour des types spécifiques d’AP. Cette étude examine desliens entre la pratique des sports de raquette, les activités rythmiques et gymnastiques, les activitésde conditionnement, les activités physiques de plaisir, des activités récréatives, le sport d’équipe etles activités quotidiennes. 214 familles ont été inclues. Les jeunes ont été recrutés à partir de la 5eet 6e année de 17 écoles du NB, Canada. Les données ont été ramassées dans le cadre du projetMATCH avec des questionnaires auto-administrés pour les jeunes et des questionnairestéléphoniques pour les parents. Des rapports de cotes (OR) et des valeurs prédictives positives etnégatives (VPP, VPN) ont été calculés. La pratique de sports de raquettes est significativementassociée entre les parents et les enfants (OR = 3,80). Aucune autre relation significative a ététrouvé (p < 0,05). La probabilité que les parents et leur enfant pratique le même type activité étaitélevé (VPP : 0,55–0,86) tandis que la probabilité que ni le parent ni l’enfant pratique un même typed’AP était bas (VPN : 0,08-0,58). Cette étude suggère que certains types d’AP pratiqués par des

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Title of the research project - Titre du projet de rechercheAssessment of the Non-Invasive Pulsewave Health Monitor^TM for Blood Pressure Determination ina Clinical Setting of Cardiovascular Catheterization/ Évaluation de l’appareil non-invasive PulsewaveHealth Monitor^TM pour la détermination de la pression artérielle dans une clinique decathétérisation cardiaque

Poster presenter - Nom de la personne qui présentera l'afficheSarah Melville



Researchers involved in this project - Chercheurs participant au projetMelville, Sarah; Lutchmedial, Sohrab; Brunt, Keith.

Abstract - RésuméIntroduction: Home blood pressure (BP) monitoring could improve outcomes and reduce healthcare costs by providing rapid, consistent clinical information, while reducing patient visits. However,the clinical utility of such digital health devices requires rigorous assessment. Here, we measuredBP indirectly using a digital oscillometric wrist cuff device, the BioSign Inc. Pulsewave HealthMonitor^TM (PHM), simultaneously with direct radial or central arterial pressure measurementsduring routine cardiovascular catheterization. Methods: Patients scheduled for catheterizationprocedures were screened the day prior (Day 1) for bilateral BP equality (±10 systolic; ±5 diastolic). Baseline BP measurements were made with the PHM, auscultatory, and an automatic upper armdevice. On the day of procedure (Day 2) simultaneous pressure measurements were made with thePHM and an intra-arterial catheter at the right radial artery (RA) and at the ascending aorta (AA). Results: PHM data was highly correlated with direct pressures at the RA and the AA (n=10;P<0.0001). PHM diastolic pressures were more precise (RA: r2=0.8688; AA: r2=0.8217) thansystolic pressures (RA: r2=0.7797; AA: r2=0.6180) but less accurate. Direct measures weresignificantly different than the PHM diastolic pressures (RA: 68.9±8.8 vs. 86.4±12.2, P<0.0001; AA:68.0±8.7 vs. 83.5±11.2, P<0.0001). Yet, no significant difference was observed between direct andWC systolic pressures (RA: 144.8±20.2 vs. 143.6±22.1, P=0.8581; AA: 126.3±16.9 vs. 136.0±19.9,P=0.1041). Conclusions: Following calibration, the WC could provide sufficiently accuratemeasures of blood pressure to be used reliably in a clinical setting or for patient self-management.Further studies will explore the deployment of such digital health devices where vital signmanagement is key to improving patient outcomes.

Introduction: La mesure de la pression artérielle (PA) chez-soi peut améliorer les résultats de soinsde santé, réduire les coûts et les visites à l’hôpital en donnant des diagnostics rapides et réguliers. Nous avons mesuré la PA au moyen d’un bracelet oscillométrique afin de comparer nos résultatsavec la pression artérielle directe et de valider l’appareil Pulsewave Health Monitor^TM Méthodes:La veille (Jour 1) on a vérifié l’égalité bilatérale de la pression artérielle (+/- 10 systolique; +/-5diastolique) des patients en attente d’une cathétérisation cardiaque. Les mesures de base ont étéfaites avec le bracelet oscillométrique, l’auscultation, et un dispositif automatique au bras. Lors dela procédure (Jour 2) les mesures ont été faites simultanément avec le bracelet oscillométrique et lecathéter intra-artériel à l’artère radiale droite (AR) et à l’aorte ascendante (AA). Résultats: On

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constate une forte corrélation entre les mesures indirectes et les mesures directes prises sur AR etAA (n=10, p<0001). Les mesures diastoliques indirectes étaient plus précises (AR: r2=0.8688; AA:r2=0.8217) que les mesures systoliques indirectes (AR: r2=0.7797; AA: r2=0.6180). Alors qu’il yavait une différence significative entre les mesures diastoliques directes et indirectes (AR: 68.9±8.8c. 86.4±12.2, P<0.0001; AA: 68.0±8.7 c. 83.5±11.2, P<0.0001), il n’y avait pas de différencesignificative entre les mesures systoliques (AR: 144.8±20.2 c. 143.6±22.1, P=0.8581; AA:126.3±16.9 c. 136.0±19.9, P=0.1041). Conclusion: Bien calibré et vérifié dans différents contextescliniques le bracelet oscillométrique sera aussi fiable que la méthode directe. Par la suite, l’appareilsera comme une aide viable dans les soins aux patients où la gestion des signes vitaux estimportante pour améliorer les résultats de soins de santé.

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Title of the research project - Titre du projet de rechercheNanoparticle delivery of curcumin to human endothelial cells demonstrates efficient uptake and noovert toxicity to signficantly induce the expression of heme-oxygenase-1

Poster presenter - Nom de la personne qui présentera l'afficheShona Philip


Education LevelMedical Students

Researchers involved in this project - Chercheurs participant au projetPhilip, Shona; Rasul, Amna; Puvvada, Naga; Brunt, Keith.

Abstract - RésuméObjective: Current interventions for atherosclerosis such as percutaneous coronary intervention arenot curative. They fail to maintain endothelial physiology and inflammation and oxidative stress stillpersist. Innovative therapies are urgently required. Advances in nanoparticle technology - nanocardiology – have demonstrated promise in advancing current cardiac therapies. Nanoparticlesare ultra-small agents that have the potential to restore a dysfunctional endothelium at a molecularlevel. Heme-oxygenase -1 (HO-1) is an antioxidative, anti-inflammatory, and cytoprotective enzymethat has the potential to protect the endothelium. Nanoparticles have the ability to encapsulate HO-1inducers like curcumin, which can allow the healing of the endothelium. Materials and Methods: Inthis research study, human umbilical vein endothelial cells (HUVECS) were treated with ahigh-density lipoprotein (HDL) nanoparticles containing curcumin payload or dir-BOA payload andwere easily detected in fluorescent payloads in cells. These treated cells were then exposed todifferent concentrations of hydrogen peroxide and TNF alpha to induce oxidative stress andinflammation. Resazurin assay was applied to determine viability. Results: It was evidenced thatthere was no significant difference between the untreated cells and the cells treated with curcuminnanoparticles or dir-BOA nanoparticles post varying hydrogen peroxide doses. Post varying dosesof TNF alpha, the viability of the treated cells were marginally improved compared to untreated cells,especially the dir-BOA nanoparticle treated cells. Conclusion: The findings of this research studyshow the potential of HO-1 inducers to protect the endothelium via nanoparticles. Nanoparticles canadvance current therapies such as percutaneous coronary interventions to protect the endotheliumon a molecular level. Further in vitro and in vivo research in necessary to explore the potential ofnanoparticles in cardiology.

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Title of the research project - Titre du projet de rechercheInfluence of childcare educators on physical activity and eating behaviours of preschool-agedchildren: A systematic review

Poster presenter - Nom de la personne qui présentera l'afficheStephanie Ward



Researchers involved in this project - Chercheurs participant au projetBélanger, Mathieu ; Donovan, Denise; Carrier, Natalie.

Abstract - RésuméAlthough familial influence on young children’s lifestyle habits is well documented, social influenceswithin the childcare setting have been largely conjectural. Over half of children under the age of fiveattend childcare services in Canada, and spend an average of 30 hours per week with theireducators. Since children learn by observing and imitating others, targeting child care educators asrole models could be an effective strategy for promoting healthy eating habits and physical activity inyoung children. In this systematic review, we assess the influence of educators on preschoolchildren’s diet and physical activity in childcare centres. Methods/Design: Six internationaldatabases were searched for quantitative (intervention or observational) peer-reviewed, English orFrench primary studies which reported on the association between childcare educators’ practices orbehaviours on preschool-aged children’s diet or physical activity habits. Risk of bias was assessedusing the Quality Assessment Tool for Quantitative Studies. Results: Twenty-three articles wereincluded in this review: thirteen measured physical activity levels, seven assessed nutrition ornutrition-related behaviours, and three measured both physical activity and nutrition. Of thesearticles, fifteen obtained a weak quality score, while seven received “moderate” ratings and only onewas assessed as “strong”. When educators led education programs, children increased theirphysical activity levels, decreased their sedentary time, improved their gross motor skills orimproved their nutrition-related behaviours or knowledge. The influence of specific educatorpractices or behaviours remains unclear. Discussion: Educator-delivered programs seem to have apositive effect on children’s physical activity and nutrition-related habits. However, it is still unclearwhat role educators play in the efficacy of these interventions and what specific practices influencechildren’s behaviours in the short and long term.

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Title of the research project - Titre du projet de rechercheAngiotensin II acutely increases lysosomal autophagy in the murine myocardium

Poster presenter - Nom de la personne qui présentera l'afficheThomas Pulinilkunnil



Researchers involved in this project - Chercheurs participant au projetTrivedi, Purvi; Weatherbee-Martin, Nathan; Kienesberger, Petra; Legare, Jean Francois;Pulinilkunnil, Thomas.

Abstract - RésuméBackground: Angiotensin II (Ang II) stimulates protein synthesis and cell growth in the myocardiumto cause hypertrophic heart failure. However it remains to be delineated whether cardiac effects ofAng II involves alterations in protein degradation. Cytosolic proteins are degraded by eithermacroautophagy or chaperone mediated autophagy (CMA) in the lysosome. Aim: We examined theacute effects of Ang II on lysosomal autophagy in the healthy myocardium. Methods and Results: 8week old C57BL6 mice were administered Ang II (2.0 μg/kg per min) for three days using osmoticminipumps. Ang II treatment augmented mean arterial blood pressure and heart weight. IncreasedmRNA expression of collagen 1A1 (Col1A1) and transforming growth factor-β (TGF-β) confirmedfibrotic remodeling following acute Ang II exposure. Furthermore, Ang II increased proteinexpression of phosphorylated mammalian target of rapamycin (mTOR), S6 kinase and ribosomalS6, suggesting hyper-activation of mTOR signaling consistent with increased protein synthesis. AngII treatment also decreased activation of AMP activated protein kinase (AMPK), the endogenousinhibitor of mTOR. Since mTOR inhibits autophagy, we expected suppression of autophagy afterAng II treatment. Surprisingly, immunoblot analysis revealed significant increase in atuophagymarkers namely microtubule associated light chain 3 (LC3-B), lysosome associated membraneprotein (LAMP-2A), heat shock cognate protein 70 (Hsc70) and cathepsin D, signifying increasedlysosomal activity and protein degradation following acute exposure to Ang II. Significance: Takentogether following acute Ang II exposure upregulation of lysosomal autophagy in the myocardium isan adaptive response to compensate for the increased protein synthesis.

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Title of the research project - Titre du projet de rechercheA work in progress: Perceived barriers and enablers held by interprofessional team membersaround collaborating when communicating through the use of information and communicationtechnologies

Poster presenter - Nom de la personne qui présentera l'afficheMonica Graves



Researchers involved in this project - Chercheurs participant au projetGraves, Monica; Doucet, Shelley; Dube, Anik; Johnson, Michel.

Abstract - RésuméNew Brunswick has the highest chronic disease costs in the country and a largely rural geographiclandscape. Research is urgently needed to advance our understanding of interprofessional practicein eHealth interventions, given the opportunities to improve access to health care services forvulnerable populations who have chronic diseases and live in remote and underserviced areas. Aspart of the Connected Voices project funded through Pfizer Canada and NBHRF, acommunity-based interprofessional mobile eHealth (mHealth) support strategy (12 weekspost-rehabilitation) is presently being piloted in a rural community as a supplement to traditionalface-to-face cardiopulmonary rehabilitation. This specific study explores health professional’sperceptions of anticipated barriers and facilitators to effectively collaborating when communicatingthrough telemedicine. This is an exploratory qualitative study using a case study design of theinterprofessional healthcare team who will later be involved in the 12 week mHealthpost-rehabilitation intervention using iPads, telephone calls, and email. Individual interviews havebeen conducted and the qualitative data is currently being analyzed using inductive thematicanalysis. Preliminary findings show that health professionals anticipate several barriers tocollaboration when communicating through telemedicine, such as lack of awareness with regards toeach other’s schedules, inability to access patient paper files, and lack of visual cues. Perceivedfacilitators to collaboration when not co-located include regular virtual team meetings, a technicianteam member available for troubleshooting, and an initial face-to-face meeting for the team. Thefindings have implications on how to promote collaboration amongst healthcare teams who are notco-located, which will be presented.

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Title of the research project - Titre du projet de rechercheEffects of Transcatheter Aortic Valve Implantation on Cognitive Function

Poster presenter - Nom de la personne qui présentera l'afficheClaudia Cote



Researchers involved in this project - Chercheurs participant au projetPaddock, Vernon; Leblanc, Heather; Archer, Brian; Ferguson, Darren; Forgie, Rand; Yip, Alexandra;Hassan, Ansar; Pelletier, Marc.

Abstract - RésuméINTRODUCTION: Aortic valve disease is a significant cause of morbidity and mortality and is oftentreated with surgical aortic valve replacement. Transcatheter aortic valve implantation (TAVI) is analternative to surgical aortic valve replacement for frail patients with severe aortic valve stenosis whoare poor surgical candidates. TAVI results in improved valve function as well as patient symptomsand functional status, however, the effect of TAVI on cognitive function is unclear. The purpose ofthis study was to determine whether TAVI is associated with a change in cognitive function in thishigh-risk patient population. METHODS: A prospective evaluation of all patients undergoing TAVIbetween April 2010 and October 2013 was performed. Cognitive function was assessed bothpreoperatively and 6 months following the procedure using the Montreal Cognitive Assessment(MOCA). RESULTS: 59 consecutive patients underwent successful TAVI procedure and survivedto 6-month follow-up. After TAVI, cognitive function assessed by MOCA displayed no significantchange (median 23, [IQR 21, 26] to 24, [21, 26], p=0.26). Analysis of cognitive domains within theMOCA demonstrated an increase in delayed recall score with no change in other cognitive domains(Table 1). CONCLUSIONS: TAVI improves aortic valve function, symptoms and functionaloutcomes. Overall cognitive function was unchanged 6 months after the procedure, with anincrease in delayed recall ability. TAVI procedure results in minimal to no change in cognitivefunction. Concerns about stroke and microscopic embolization causing cognitive dysfunction are notsupported by this current study, and should not deter use of TAVI.

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