Poster de LuisSMPR11LRHxacademic.uprm.edu/~lrios/Posters/Poster de LuisSMPR11LRHx...Poster de...
1
The use of Anaerobic batch reactors (ABRs) for the anaerobic digestion involves several metabolic reactions from different functional microbial groups. The relevance of using an ABR take root in contributing to resolve the solid waste disposal and fossil fuels dependence problems. We developed two ABRs; for ABRa we selected an anaerobic microbial community (AMC) with functional roles of collagen degradation coupled with methanogenesis, and for ABRb we selected and mixed two AMCs; one with the functional role of collagen degradation, and the other with the role of syntrophic volatile fatty acid (VFAs) degradation. In this research, we tested which ABR biotransformed more efficiently the collagen based on methane production, the VFAs production or consumption, and by comparing the bacterial and methanogenic population among the ABRs using Denaturing Gradient Gel electrophoresis (DGGE). Our results showed that ABRa biotransform 11% of the waste in to methane generating 2.7 Watts while ABRb biotransform only 4.4% of the waste in to methane generating 3.7 Watts. DGGE analysis showed similarity on the ABRs bacterial population, suggesting a parallel selecting process in these populations regardless of their origin. However, differences were detected between the ABRs methanogenic populations through time. It appears that the ABRs converged on the same hydrogenotropic methanogen Methanospirillum hungatei. Furthermore, the accumulation of acetate, the most abundant intermediary, in ABRb suggests that an acetoclastic methanogen is absent. In conclusion, using a single AMC in an ABR is more efficient to biotranform collagen to methane. The use of anaerobic batch reactor for the bioconversion of an Industrial solid waste to methane Luis A. Piñero García* and Luis A. Ríos Hernández University of Puerto Rico at Mayagüez, Biology Department, Anaerobic Solutions Lab B256 SB medium Anaerobic chamber Inoculation of the Anaerobic batch reactors DGGE HPLC GC Abstract Abstract Abstract Methodology Methodology Methodology Blended A L CH 4 Waste anaerobic solutions lab biotranform collagen to methane. H 0 : The ABR developed with an AMC with functional roles of collagen degradation coupled with methanogenesis will biotransform more efficient the collagen into methane than the ABR developed with a mixture of different AMCs. H 1 : The ABR developed with an AMC with functional roles of collagen degradation coupled with methanogenesis will not biotransform more efficient the collagen into methane than the ABR developed with a mixture of different AMCs. Finstein, M. (2010). Anaerobic digestion variants in the treatment of solid wastes. Microbe, 5(4):151-155. Jain M., and Zeikus J. (1989). Bioconversion of Gelatin to Methane by a Coculture of Clostridium collagenovorans and Methanosarcina barkeri. Applied and Environmental Microbiology, 55:366-371. Watanabe T., Asakawa S., Nakamura S., Nagaoka K., Kimura M. (2004). DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. Elsevier, 232:153-163. • Industrial solid wastes such collagen can be biotransformed to methane. • The bacterial diversity within the reactors were similar as showed by DGGE. •DGGE analysis showed that Methanospirillum hungatei was the only hydrogenotrophic methanogen responsible for the production of methane. •An accumulation of the most abundant intermediary (acetate) in ABRb suggests low efficiency in the degradation of this intermediary possibly suggesting syntrophic metabolism. • The use of one anaerobic microbial community with functional roles of collagen degradation coupled with methanogenesis is more efficient that using a mixture of AMCs. DGGE Bact 16S DGGE Bact 16S A- ARBa (1 day) B- ABRb (1 day) C-ABRa (45 day) D-ABRb (45 days) E-ABRa (90 days) F-ABRb (90 days) G- Syntrophus aciditrophicus A B C D E F G DGGE HPLC GC Introduction Introduction Introduction Hypothesis Hypothesis Hypothesis Results Results Results Conclusion Conclusion Conclusion References References References In natural environments, the anaerobic digestion of organic matter such as proteins, carbohydrates, and lipids involves a complicated process of several metabolic reactions from at least three different functional microbial groups that constitutes as a whole an anaerobic microbial community (Ward, A. J. 2008). The heart of the digester depends on the bioconversion potential reflected in the amount of methane produced and the stability of the remaining digestate (Finstein, M. 2010). The main objective of this research was to prove that an industrial solid waste (collagen) could be transformed into a renewable energy source using an anaerobic batch bioreactor. For our goal, it was essential to develop two ABRs with selected functional anaerobic microbial communities. In this study, we determined which ABR biotransformed more efficiently the collagen into methane, based on the production of methane and VFA’s; the subsequent consumption of the VFA’s, and by comparing the stability of the bacterial and methanogenic population within the ABRs. 8% acrylamide, 12h, 60ºC,100v (30-50%) DGGE Archea 16S DGGE Archea 16S A B C D E F G H I A- ARBa (1 day) B- ABRb (1 day) C-ABRa (45 day) D-ABRb (45 days) E-ABRa (90 days) F-ABRb (90 days) G- Empty H- Empty I- Methanospirillum hungatei 8% acrylamide, 12h, 60ºC,100v (50-70%)
Transcript of Poster de LuisSMPR11LRHxacademic.uprm.edu/~lrios/Posters/Poster de LuisSMPR11LRHx...Poster de...
The use of Anaerobic batch reactors (ABRs) for the anaerobic digestioninvolves several metabolic reactions from different functional microbialgroups. The relevance of using an ABR take root in contributing toresolve the solid waste disposal and fossil fuels dependence problems.We developed two ABRs; for ABRa we selected an anaerobic microbialcommunity (AMC) with functional roles of collagen degradation coupledwith methanogenesis, and for ABRb we selected and mixed two AMCs;one with the functional role of collagen degradation, and the other withthe role of syntrophic volatile fatty acid (VFAs) degradation. In thisresearch, we tested which ABR biotransformed more efficiently thecollagen based on methane production, the VFAs production orconsumption, and by comparing the bacterial and methanogenicpopulation among the ABRs using Denaturing Gradient Gelelectrophoresis (DGGE). Our results showed that ABRa biotransform11% of the waste in to methane generating 2.7 Watts while ABRbbiotransform only 4.4% of the waste in to methane generating 3.7 Watts.DGGE analysis showed similarity on the ABRs bacterial population,suggesting a parallel selecting process in these populations regardlessof their origin. However, differences were detected between the ABRsmethanogenic populations through time. It appears that the ABRsconverged on the same hydrogenotropic methanogen Methanospirillumhungatei. Furthermore, the accumulation of acetate, the most abundantintermediary, in ABRb suggests that an acetoclastic methanogen isabsent. In conclusion, using a single AMC in an ABR is more efficient tobiotranform collagen to methane.
The use of anaerobic batch reactor for the bioconve rsion of an Industrial solid waste to methane Luis A. Piñero García* and Luis A. Ríos Hernández
University of Puerto Rico at Mayagüez, Biology Depa rtment, Anaerobic Solutions Lab B256
SB medium
Anaerobic chamber
Inoculation of the Anaerobic batch
reactors
DGGE HPLC GC
AbstractAbstractAbstract MethodologyMethodologyMethodology
Blended
A LCH4
Waste
anaerobic solutions lab
biotranform collagen to methane.
H0: The ABR developed with an AMC with functional roles of collagendegradation coupled with methanogenesis will biotransform moreefficient the collagen into methane than the ABR developed with amixture of different AMCs.
H1: The ABR developed with an AMC with functional roles of collagendegradation coupled with methanogenesis will not biotransform moreefficient the collagen into methane than the ABR developed with amixture of different AMCs.
Finstein, M. (2010). Anaerobic digestion variants in the treatment of solid wastes. Microbe, 5(4):151-155.
Jain M., and Zeikus J. (1989). Bioconversion of Gelatin to Methane by a Coculture of Clostridium collagenovoransand Methanosarcina barkeri. Applied and Environmental Microbiology, 55:366-371.
Watanabe T., Asakawa S., Nakamura S., Nagaoka K., Kimura M. (2004). DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. Elsevier, 232:153-163.
• Industrial solid wastes such collagen can be biotransformed to methane.
• The bacterial diversity within the reactors were similar as showed by DGGE.
•DGGE analysis showed that Methanospirillum hungatei was the onlyhydrogenotrophic methanogen responsible for the production of methane.
•An accumulation of the most abundant intermediary (acetate) in ABRb suggests lowefficiency in the degradation of this intermediary possibly suggesting syntrophicmetabolism.
• The use of one anaerobic microbial community with functional roles of collagendegradation coupled with methanogenesis is more efficient that using a mixture ofAMCs.
DGGE Bact 16SDGGE Bact 16S
A- ARBa (1 day)
B- ABRb (1 day)
C-ABRa (45 day)
D-ABRb (45 days)
E-ABRa (90 days)
F-ABRb (90 days)
G- Syntrophus aciditrophicus
A B C D E F G
DGGE HPLC GC
IntroductionIntroductionIntroduction
HypothesisHypothesisHypothesis
Results Results Results ConclusionConclusionConclusion
ReferencesReferencesReferences
In natural environments, the anaerobic digestion of organic matter suchas proteins, carbohydrates, and lipids involves a complicated processof several metabolic reactions from at least three different functionalmicrobial groups that constitutes as a whole an anaerobic microbialcommunity (Ward, A. J. 2008). The heart of the digester depends onthe bioconversion potential reflected in the amount of methaneproduced and the stability of the remaining digestate (Finstein, M.2010). The main objective of this research was to prove that anindustrial solid waste (collagen) could be transformed into a renewableenergy source using an anaerobic batch bioreactor. For our goal, itwas essential to develop two ABRs with selected functional anaerobicmicrobial communities. In this study, we determined which ABRbiotransformed more efficiently the collagen into methane, based on theproduction of methane and VFA’s; the subsequent consumption of theVFA’s, and by comparing the stability of the bacterial and methanogenicpopulation within the ABRs.
8% acrylamide, 12h, 60ºC,100v (30-50%)
DGGE Archea 16SDGGE Archea 16S
A B C D E F G H I A- ARBa (1 day)
B- ABRb (1 day)
C-ABRa (45 day)
D-ABRb (45 days)
E-ABRa (90 days)
F-ABRb (90 days)
G- Empty
H- Empty
I- Methanospirillum hungatei8% acrylamide, 12h, 60ºC,100v (50-70%)
