Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS...
-
Upload
magdalen-robertson -
Category
Documents
-
view
225 -
download
0
Transcript of Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS...
![Page 1: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/1.jpg)
Population Genetics of Puccinia coronata f. sp.
avenae
Hattie Dambroski and Martin CarsonHattie Dambroski and Martin CarsonUSDA-ARS
Cereal Disease LaboratorySaint Paul, MN
![Page 2: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/2.jpg)
Oat Crown Rust
![Page 3: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/3.jpg)
Puccinia Pathway
![Page 4: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/4.jpg)
Objective
To study the diversity and population structure of Puccinia coronata.
• sexual populations in the north
• asexual populations in the south
![Page 5: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/5.jpg)
•DNA isolated from germinated and non-germinated urediniospores
•Five DNA samples isolated from aeciospores
•41 dinucleotide microsatellites
•Target sequence (CA)n
•Highly Polymorphic, allelic diversity ranges from 2 to 22
Microsatellites
![Page 6: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/6.jpg)
Populations
• Northern Populations• MN, ND, SD, WI, NE
• Southern Populations• NC, FL, LA, AL
• Texas
• California
• Foreign Populations• Brazil, Israel, Russia, Egypt, Australia, Mexico, Canada, Uruguay, Turkey, Argentina
![Page 7: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/7.jpg)
Isolates
CA
TX
SD
ND MN
LAFL
NC
Surveyed 75 isolates so far
NE
WI
AL
![Page 8: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/8.jpg)
Statistics
HO ranged from 0.014 to 0.973
HE from 0.166 to 0.742
11 loci are in Hardy-Weinberg equilibrium (P<0.05)
![Page 9: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/9.jpg)
Preliminary Results
AMOVA showed most molecular variance within populations vs. among populations
Neighbor joining tree based on genetic distance
![Page 10: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/10.jpg)
Tree Results
1.Group with little genetic differentiation forming between north, south and Texas populations (asexual pop.)
2.Northern aecial isolates form a group with greater genetic differentiation between isolates (sexual pop.)
3.California isolates forming own group with great genetic differentiation from other groups
![Page 11: Population Genetics of Puccinia coronata f. sp. avenae Hattie Dambroski and Martin Carson USDA-ARS Cereal Disease Laboratory Saint Paul, MN.](https://reader035.fdocuments.in/reader035/viewer/2022062217/56649d8c5503460f94a7428d/html5/thumbnails/11.jpg)
Things yet to do…..
•Finish U.S. Isolates
•Screen Foreign Isolates
•Screen Markers on other Rust species
•Analyze data