Announcement of population data

Population genetics of nine STR loci from

Baoan population in NW China

Teng Chen a,b, Jianghua Lai a,b, Yanjiong Chen b, Shengbin Li a,b,*

aDepartment of Forensic Science, Medical College of Xi’an Jiaotong University, Xi’an 710061, PR ChinabKey Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University,

Ministry of Education, Xi’an Shaanxi 710061, PR China

Received 30 September 2004; accepted 4 March 2005

Available online 11 November 2005

Abstract

Allele frequencies for the nine short tandem repeat (STR) loci (D3S1358, FGA, TH01, D7S820, VWA, CSF1PO, D5S818,

D13S317, TPOX) were determined in a sample of 120 unrelated individuals of the Baoan ethnic group living in northwest China.

The values of observed heterozygosity (H), discrimination power (DP), a priori chance of exclusion (CE) and polymorphism

information content (PIC) were calculated. All loci were in accordance with Hardy–Weinberg equilibrium (P = 0.05). The

obtained frequency distributions were compared with other previously reported population data.

# 2005 Elsevier Ireland Ltd. All rights reserved.

Keywords: Forensic science; Short tandem repeats (STR); Population genetics; China

www.elsevier.com/locate/forsciint

Forensic Science International 157 (2006) 218–220

1. Populations

Baoan is an ethnic group in Gansu Province of Northwest

China with a small population of about 11,683. The Baoan

ethnic group speaks Chinese and does not have its own

distinct characteristics in terms of spoken and written lan-

guage. They embrace Islam, and are thought to originate

from Mongolia. In this study, blood samples (n = 120) of the

Baoan ethnic group were collected from unrelated healthy

individuals who self-declared having the ethnic origin of

over four generations.

2. Extraction

Genomic DNA was extracted by the salting out method

and was quantified with agarose gel electrophoresis.

* Corresponding author. Tel.: +86 29 82656244;

fax: +86 29 82656244.

E-mail address: shbinlee@mail.xjtu.edu.cn (S. Li).

0379-0738/$ – see front matter # 2005 Elsevier Ireland Ltd. All rights

doi:10.1016/j.forsciint.2005.03.015

3. PCR

Amplification was performed using 5ng genomic DNA

template according to manufacture’s protocol [1]

(AmpF‘STR1 ProfilerTM PCR Amplification kit, Applied

Biosystems, Foster City, USA).

4. Typing

The amplified DNA products were typed using the ABI

PrismTm377 DNA sequencer (PE Applied Biosystems). Data

analysis and allele designation were carried out using the

GeneScanTM and GenetypeTM softwares.

5. Analysis of data

To check genetic stability of the Baoan ethnic groups, the

data of the allele frequency were analyzed based on Hardy–

Weinberg equilibrium test. The discrimination power (DP), a

reserved.

T. Chen et al. / Forensic Science International 157 (2006) 218–220 219

Table 1

Allele frequency for nine STR loci in Baoan population of China (n = 120)

Allele D3S1358 VWA TH01 FGA TPOX CSF1PO D5S818 D7S820 D13S317

5 – – – – – – – – –

6 – – 0.0914 – – – – – –

7 – – 0.3118 – 0.0054 – 0.0108 – –

8 – – 0.0591 – 0.4892 – 0.0054 0.1559 0.3118

9 – – 0.4516 – 0.1452 0.0430 0.0753 0.0914 0.1183

9.3 – – 0.0806 – – – – – –

10 – – 0.0054 – 0.0269 0.2419 0.2527 0.1452 0.1613

11 – – – – 0.2796 0.2366 0.3871 0.2796 0.2043

12 – – – – 0.0538 0.3978 0.1505 0.2419 0.1720

13 0.0054 – – – – 0.0645 0.0914 0.0860 0.0215

14 0.0484 0.1774 – – – 0.0161 0.0269 – 0.0108

15 0.3602 0.0699 – – – – – – –

15.2 – – – – – – – – –

16 0.2473 0.2097 – – – – – – –

17 0.2688 0.2366 – – – – – – –

18 0.0699 0.2258 – 0.0215 – – – – –

19 – 0.0591 – 0.0699 – – – – –

20 – 0.0215 – 0.0430 – – – – –

20.2 – – – – – – – – –

21 – – – 0.0860 – – – – –

21.2 – – – 0.0054 – – – – –

22 – – – 0.1720 – – – – –

22.2 – – – 0.0108 – – – – –

23 – – – 0.2043 – – – – –

23.2 – – – 0.0161 – – – – –

24 – – – 0.2581 – – – – –

24.2 – – – 0.0054 – – – – –

25 – – – 0.0645 – – – – –

25.2 – – – – – – – – –

26 – – – 0.0269 – – – – –

27 – – – 0.0161 – – – – –

28 – – – – – – – – –

H 0.7296 0.8088 0.6804 0.8419 0.6578 0.7210 0.7488 0.8022 0.7908

PD 0.8704 0.9324 0.8524 0.9529 0.8218 0.8668 0.8810 0.9228 0.9151

CE 0.5949 0.7614 0.5554 0.8306 0.5048 0.5577 0.6137 0.7030 0.6634

PIC 0.7194 0.7970 0.6740 0.8418 0.6471 0.7017 0.7217 0.7881 0.7827

P 0.9660 0.9950 0.890 0.9990 0.9960 0.9970 0.9570 0.9920 0.8370

H, observed heterozygosity; DP, discrimination power; CE, a priori chance of exclusion; PIC, polymorphism information content; P, Hardy–

Werinberg equilibrium, chi-square test.

priori chance of exclusion (CE) and the heterozygosity (H)

were calculated [2,3]. The chi-square test after Bonferroni

correction was used for population differentiation.

6. Results

DP, CE, H, P-values from the chi-square test for Hardy–

Weinberg equilibrium (P) and the polymorphism informa-

tion content (PIC) for the nine STR loci in Baoan ethnic

groups from China are given in Table 1.

7. Access to data

The complete data are available for any interested reader

upon request made to Shengbin Li, M.D., Department of

Forensic Science, Medical College of Xi’an Jiaotong Uni-

versity, Xi’an 710061, PR China.

8. Other remarks

All loci were found to be highly polymorphic and no

deviation from Hard–Weinberg equilibrium was observed in

the population studied in the present research (P = 0.05).

The obtained frequency distributions were compared with

the other previously reported population data from Japanese

population [4], Sinkiang NW China [5], Dongxiang ethnic

group in Gansu province of northwest China [6] and Han

nation population in Shandong Province of China [7],

respectively. No significant differences were observed using

the chi-square test after Bonferroni correction (P = 0.01).

T. Chen et al. / Forensic Science International 157 (2006) 218–220220

This paper follows the guidelines for publication of

population data requested by the journal [8].

Acknowledgments

This study is supported by National Nature Science

Foundation of China (No. 39970401).

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