PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle ([email protected])[email protected].
-
Upload
linda-cameron -
Category
Documents
-
view
214 -
download
0
Transcript of PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle ([email protected])[email protected].
![Page 2: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/2.jpg)
Journal club format
Why?
- Practice how to prepare papers and express ideas correctly.
- Training in science debate.
- Practice presentation: research seminar.
![Page 3: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/3.jpg)
![Page 4: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/4.jpg)
![Page 5: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/5.jpg)
TARGETED PROTEIN DEGRADATION
Lectures: January 15, 17
Exam: January 22
![Page 6: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/6.jpg)
![Page 7: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/7.jpg)
![Page 8: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/8.jpg)
Central dogma of genetics
![Page 9: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/9.jpg)
Central dogma of genetics
Regulation of an organisms differentiation and responses to the environment are the result of changes in gene expression.
OLD THEORY: Regulation of gene expression is predominantly controlled at the transcription level.
NEW THEORY: Regulation of gene expression is the result of a combination of protein synthesis (transcription/translation) and protein degradation controls.
![Page 10: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/10.jpg)
DNA RNA PROTEIN
Light
Light responses
EXAMPLE Day/night cycle: day light activates gene expression
![Page 11: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/11.jpg)
DNA RNA PROTEIN ????
Dark
Light responses ?
Day/night cycle: During night time, light-inducible genes are down-regulated
However, regulatory proteins that promote the light response need to be removed in the dark. Otherwise, they would interfere with the dark response.
![Page 12: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/12.jpg)
PhotomorphogenesisHY5: a transcriptional activator of photomorphogenic development
5 days old Arabidopsis seedlings grown under a range of light intensities
Light intensity
(Osterlund et al., 2000)
Western blot using HY5 antiserum
![Page 13: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/13.jpg)
Ethylene signalingEIN3: a transcriptional activator of ethylene responses
4 days old Arabidopsis seedlings grown in the dark on a range of concentrations of the ethylene precursor ACC
(Guo and Ecker, 2003)
Western blot using EIN3 antiserum
![Page 14: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/14.jpg)
Proteolysis
Errors: Misfolded: Cleaved: AA supply: Regulation:
PRQTEIN PRO
T
EIN
PRO + PROTEIN
PROTEIN
PR
OT
E
IN
PROPROTEIN
PR
QT
E
IN P
R
OT
E
IN
PROTEIN
PROTEIN
PR
OT
E
IN
![Page 15: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/15.jpg)
Non-specific Proteolysis
PROTEIN
PR
OT
E
IN
PROTEIN
PR
OT
E
IN P
R
OT
E
IN
PROTEIN PROTEIN
PR
OT
E
IN
PROTEIN
PR
OT
E
IN
PROTEIN
PR
OT
E
IN
![Page 16: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/16.jpg)
Specific or Targeted Proteolysis
PROTEIN PROTEIN PROTEIN
PR
OT
E
IN
PROTEIN PROTEINPROTEIN
![Page 17: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/17.jpg)
Conditional Proteolysis
PROTEIN PROTEIN PROTEIN
PR
OT
E
IN
PROTEIN PROTEINPROTEIN
DARK
![Page 18: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/18.jpg)
Conditional Proteolysis
PROTEIN PROTEIN*Kinase
Phosphatase
PRO
TE
IN
Many cases of conditional proteolysis involve secondary modifications of target proteins. For example: phosphorylation/dephosphorylation.
![Page 19: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/19.jpg)
Conditional Proteolysis
PROTEIN PROTEIN*Kinase
Phosphatase
PRO
TE
IN
Many cases of conditional proteolysis involve secondary modifications of target proteins. For example: phosphorylation/dephosphorylation.
![Page 20: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/20.jpg)
Conditional Proteolysis
PROTEIN PROTEIN PROTEIN
PR
OT
E
IN
PROTEIN PROTEINPROTEIN
Signal
Receptor
*
X?
![Page 21: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/21.jpg)
![Page 22: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/22.jpg)
![Page 23: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/23.jpg)
![Page 24: PLS 623: Physiology of Plants II Spring Semester 2014 Jan Smalle (jsmalle@uky.edu)jsmalle@uky.edu.](https://reader035.fdocuments.in/reader035/viewer/2022062803/56649f4f5503460f94c70fb7/html5/thumbnails/24.jpg)
[Vierstra, 2003 TIPS]