plasmid isolation.HARIS
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HARIS PMSC BIOTECHNOLOGYM.A.COLLEGEKOTHAMANGALAM
ISOLATION OF PLASMID DNA BY ALKALINE LYSIS WITH SDS
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• Plasmid is an extra chromosomal DNA molecule which is capable of self replication.
• It is circular and double stranded.
• Plasmids naturally occur in bacteria but are also found in eukaryotic yeast.
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HOW PLASMIDS ARE ISOLATED???
3 MAIN STEPS:-
1. CELL LYSIS
2. DEPROTEINATION & REMOVAL OF Chr.DNA
3. PRECIPITATION OF PLASMID DNA
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FOR WHAT ????
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