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PlantibodiesPresented by:-

KAMBLE A.P.

7th sem

(BED11ABT15B)

What are plantibodies ??

A plantibody is a Antibody produce by genetically modified plant.

Antibody are part of Animal immune system, and produce in plant by transforming them with Antibody genes from Animals.

Cont….

This was first done in 1989, with a mouse antibody made by Tobacco plant.

Although plant do not naturally make antibody, Plantibody have been shown to function in the same way as normal antibodies.

Antibody factories

Plant are being used as antibody factories, using their endomembrane and secretory system to produce large amount of clinically visible proteins.

Antibodies can be expressed in plant as either full –length molecules or as smaller fragment.

Methods for plantibody production

Using transformation and translent expression to introduce new genes into a host cell.

Targetting to the APOPLASM by the tagging with a small peptide sequence.

Cont….

Single molecule added to target the protein to Endoplasmic Reticulum (E.R); to ensure correct folding of the protein.

Higher protein level obtained in the E.R and the apoplasm as compared to the crystol.

Purification of plantibodies

Purification can be purified cheaply in large quantity

Transgenic seeds assure excellent storage properties and due to limited range of endogenous proteins in seed, separation of plantibodies is less complicated

There is no risk of srading animal disease to human as the antibody produced by plants.

Technique for purification of plantibodies

Filtration

Immunofluoresence

Chromatography

Difiltration

Polymer fusion.

Evaluation technique for the Plantibodies:-

RIA (Radioimmunoassay)

ELISA (Enzyme linked immuno sorbant assay)

Immunofluorence southern blot analysis.

Production cost of Plantibodies

Advantage:- Large-scale production of plantibodies for

immunotherapy

Benificial to human, plants as well as economy.

No risk of transmitting diseases to human

Eliminate the toxic compound;

Application:-

Antibodies generated by plant are cheaper, easier to manage, and safer to use than those obtained from animals.

Treatment of illness such as immune disorder, cancer and inflammatory disease.

Plantibodies are close to passing clinical trail.

Tobacco-derived ‘’PLANTIBODIES’’ enter the fight against Ebola.