Plant Protecting Agents In Hops

Research, Analytics and Certainty

Research

Research and method development for hops matrixes was already done at Eurofins in 2009. The methods were validated and implementated the following year.

Analytics

Our method is based on the official multimethod DFG S19(1) and Klein & Adler(2). Using an additional clean up in adsorption phases, substances which interfere in the analysis such as hops resin are separated.

1. Homogenized samples + internal standards

2. Extraction + clean up in SPE phases

3. concentrated eluate measurement

Using extract clean ups (pic. 1) it is possible to meet the maximum residue requirements according to Regulation (EG) Nr. 396/2005 with low limits of quantification.

Using a method especially developed for hops, 564 pesticides in cone hops, pellets (P90/P45) and extracts (CO2/Ethanol) are tested in one screening. The screening goes beyond the spectrum of allowed active agents and recognizes cross-contamination through drift from neighboring cultures (e.g. fruit).

Along with the hops pesticide screening we also offer our clients a growing portfolio of individual analyses. At this time we are mainly concentrating on testing for flumioxazine, chlorates, bromoxynil, milbemectin, glyphosate, deiquat, paraquat, sulphur, MCPA, clopyralid, prothioconazole, dithianon, copper and fosetyl-al.

Picture1: extract-clean up

GC/MS & GC/HR-MS (TOF) LC/MS/MS

raw extract

GC measurement vial

raw extract

LC measurement vial

Eurofins Food & Feed Testing Germanyservice@eurofins.de – www.eurofins.de

Hopfen CO2 Extrakt z-Score

Pestizide\Jahr 2011 2012 2013 2014 2015

Azoxystrobin 0,41 0,43 0,74 0,93 0,35

Bifenthrin - - - - -0,83

Boscalid 0,62 0,42 1,25 1,28 -0,57

Captan - -1,10 0,23 0,58 -0,60

Dimethomorph 0,08 0,36 1,07 0,66 -0,92

Fenpyroximat - - - - 0,43

Flonicamid 0,29 -0,20 0,43 0,91 -0,81

TFNA - - - - -0,80

TFNG - - - - -0,80

Fluopicolide 0,36 -0,09 1,36 0,33 0,00

Folpet -0,10 0,60 0,61 0,75 0,66

Hexythiazox - - - - -0,82

Imidacloprid - - - - -0,82

lambda Cyhalothrin - - - -0,78 -0,59

Mandipropamid - - 1,03 1,91 -0,80

Metalaxyl 0,00 0,88 0,69 0,11 -0,47

Myclobutanil 0,28 0,28 0,89 1,07 -0,85

Pyraclostrobin 0,28 0,61 0,81 -1,14 -0,70

Quinoxyfen 0,50 0,18 0,85 0,61 -0,68

Spirodiclofen 0,44 -0,17 0,72 1,19 -0,81

Triadimenol 0,68 0,25 0,92 -0,07 -0,68

Trifloxystrobin 0,11 0,70 0,71 0,11 -0,76

Table 1: z-Score of the ring analyzes from 2011 to 2015

The information included in this publication is based on our current knowledge and was thoroughly verified. Nevertheless there is no guarantee for it to be up-to-date,complete and correct. Eurofins is not to be held liable for damages or injury, which are connected to the use of this information. Especially the confirmation of legaldetails is up to the user. All offers are non-binding and without obligation.

16G

TZ

W11

Certainty

The precision and repeatability of the methods is regularly demonstrated by laboratory ring tests.

Table 1 shows the distribution of Eurofins z-scores in the ring tests of the Hops Analysis Working Group (Arbeitskreis Hopfenanalytik - AHA). This qualification, as well as the ca. 1.500

hops screenings done yearly, show that Eurofins is highly competent in residue analysis.

Literature:

[1] Official collection of testing procedures according

to §64 LFGB L00.00-34

[2] Official collection of testing procedures according

to §64 LFGB L00.00-113