PLANNING AND CONDUCT OF FIELD WORK BY PLANNING AND CONDUCT OF FIELD WORK BY DRC TEAM IN COLLABORATION WITH CONGO DRC TEAM IN COLLABORATION WITH CONGO

PROJECTPROJECT

By José Justin MBIMBI MAYI MUNENEBy José Justin MBIMBI MAYI MUNENE

COMPOSITION OF TEAM IN CONGO PROJETCOMPOSITION OF TEAM IN CONGO PROJET

American team, DRC team and Congo teamAmerican team, DRC team and Congo team

In DRC teamIn DRC team

Tshibwabwa, Tshibwabwa,

Musibono, Musibono,

Monsebula and Mbimbi .Monsebula and Mbimbi .

One of us have a particular work in Congo Project ( WWW//research. One of us have a particular work in Congo Project ( WWW//research. amnh. org/ichthyology/congo/ ). amnh. org/ichthyology/congo/ ).

For myself, I’m a collaborator for field work and actually I make For myself, I’m a collaborator for field work and actually I make researches in Lulua River in collaboration with other members of researches in Lulua River in collaboration with other members of Congo Project.Congo Project.

NEED OF INFORMATION BY AMERICAN TEAM NEED OF INFORMATION BY AMERICAN TEAM FOR SOME CONGO TRIBUTRIES /SUB-FOR SOME CONGO TRIBUTRIES /SUB-

TRIBUTRIESTRIBUTRIES

• they send funds and equipments they send funds and equipments

-cast -net, -cast -net,

-deep- net -deep- net

-rotenone, -rotenone,

-small tube containing pure -small tube containing pure alcohol for alcohol for

DNA samplingDNA sampling

PLANNING AND CONDUCT OF FIELD WORKPLANNING AND CONDUCT OF FIELD WORK

• Before each field trip Before each field trip

- the permission to the University authorities - the permission to the University authorities

- establish a calendar for field work- establish a calendar for field work

Monday wendsday Thursday Saturday Sunday

25 26 27 28 29

19 20 21 22

30 1 2 3 4 5 6

7 8 9 10 11 12 13 7 8 9 10 11 12 13

14 15 16 17 18 19

Légende

Done Kinshasa, June 14th 2008 By José-Justin MBIMBI MAYI MUNENE

Travel to Kananga KanangaKananga

16 Lulua (Katende II)

Sampling Lu Nsan

Travel to Tblu

Sampling to Tblu

Lulua (Katende I)

Return to Kananga

Lulua to bridge pont

Rivière Fwa

Lulua (Beya)

Rivière au choix

Préparation matérielle

Retour à Kinshasa

19

20

25

27

4

TRAVEL TO THE SITE CHOSEN FOR SAMPLINGTRAVEL TO THE SITE CHOSEN FOR SAMPLING

• PROTOCOLPROTOCOL

-the order of mission is presented to the -the order of mission is presented to the

locality chief locality chief

- explain the importance of my research - explain the importance of my research

-the different fishing methods are -the different fishing methods are

presented and explained too. presented and explained too.

FISHERMENFISHERMEN

• choose two or three fishermen who would help us to find the choose two or three fishermen who would help us to find the suitable sampling sites and assist us in catching fishes. suitable sampling sites and assist us in catching fishes.

• Before sampling, I use to begin to present and explain unto Before sampling, I use to begin to present and explain unto them the different methods that they will use for sampling and them the different methods that they will use for sampling and how to do it.how to do it.

• I ask them to explain me :I ask them to explain me :

- which kind of technical they practically use to catch fish, - which kind of technical they practically use to catch fish,

-in which habitat (place) of the river they catch fish -in which habitat (place) of the river they catch fish

- the best period for capturing fish in they site.- the best period for capturing fish in they site.

SAMPLING TECHNICALSAMPLING TECHNICAL

• For sampling, we usually use For sampling, we usually use cast -net (in rain season or dry season)cast -net (in rain season or dry season)

Deep- net ((in rain season or dry season) Deep- net ((in rain season or dry season)

rotenonerotenone ( dry season) ( dry season)

FISHES COLLECTEDFISHES COLLECTED

• For each sampling, the specimens are divided (separated) in two groupsFor each sampling, the specimens are divided (separated) in two groups

-DNA sampling -DNA sampling

- for taxonomic studies)- for taxonomic studies)

• DNA SAMPLINGDNA SAMPLING

Two or four specimens are selected for DNA sampling for Two or four specimens are selected for DNA sampling for

each species.each species.

Each selected specimen is tagged with a unique Each selected specimen is tagged with a unique

plastic label (where we have a number code. plastic label (where we have a number code.

The pelvic fin is taken and preserved in a small tube The pelvic fin is taken and preserved in a small tube

containing pure containing pure alcohol alcohol .Each tube is labelled with the .Each tube is labelled with the

same number as the label which is tagged to the specimen.same number as the label which is tagged to the specimen.

PRESEVATION SPECIMENPRESEVATION SPECIMEN

• The specimens selected and labelled for DNA samples The specimens selected and labelled for DNA samples

plus the specimens for the taxonomic studies are then plus the specimens for the taxonomic studies are then

fixed and preserved in formalin (10%)fixed and preserved in formalin (10%)

SITE INFORMATIONSITE INFORMATION• All informations about geographic coordinates (latitude & longitude) All informations about geographic coordinates (latitude & longitude)

are taken with a GPS, date, name of site are consigned in copy are taken with a GPS, date, name of site are consigned in copy book for work field (See modal)book for work field (See modal)

• Pictures are taken for each site of sampling to give an idea on the Pictures are taken for each site of sampling to give an idea on the aquatic biotope and surrounding habitat (See a modal)aquatic biotope and surrounding habitat (See a modal)

N° Site Geog. Coord. Date Sample name Photo Code DNA 6°14’82’’S

22°28’80 E 30/6/2008 S5E1 Telogramma monogramma Ok 132

Lulua (Kasende)

Telogramma monogramma 133

Telogramma monogramma 134

DESTINATION OF FISFES COLLECTEDDESTINATION OF FISFES COLLECTED

• All fish collected and DNA sample are expedited to All fish collected and DNA sample are expedited to the AMNH for identification.the AMNH for identification.