Phytosterols
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Transcript of Phytosterols
PHYTOSTEROLS
-AMMAR BABAR -T.Y. B.Tech (Foods)-11FET1009-ICT ( MUMBAI)[email protected]
CONTENTS
FACTS ROLE OF NUTRACEUTICALS DIFFERENTIATION INTRODUCTION CHEMICAL STRUCTURES EXTRACTION MECHANISM PRODUCTS REGULATIONS NEWS AND RESEARCH
FACTS!!!
India ranks No.1 in the world for number of cardiac patients, with 10% of the population affected.
The US and Europe are jointly No.2 with 7% each.
WHO expects heart disease to be the single greatest killer in India by 2015.
Highest growth of heart disease is among young corporate executives. Over 40% of urban Indians have high lipid levels.
Additionally, 31% of urban Indians are either overweight or obese. Costs related to heart disease exceed 60 billion dollars per year.
ROLE OF NUTRACEUTICALS
Too much of cholesterol in your blood, it can build up on the walls of your arteries causing various heart related problems
Healthy diet plays an important role in lowering the blood cholesterol levels.
Functional food having cholesterol lowering action will be effective in lowering the risk of heart diseases.
Thus we can have maximum benefits without modification of diet.
Chemical drugs have many side-effects hence use of natural substances (Nutraceuticals) is increasing.
PHYTOSTEROL
It was recognized in 1950s that plant sterols lower serum concentrations of cholesterol
Naturally occur only in plant and soluble only in alcohol
Phytosterol has two classes : sterols and stanols.
Phytosterols, phytostanols and their esters (200types) are a group of steroid alcohols and esters
They hormones produced by the plants to help in adaptation to different temperatures.
Chemical Structure
• They consist of a steroid skeleton with a hydroxyl group attached to the C-3 atom of the A-ring and an aliphatic side chain attached to the C-17 atom of the D-ring. Sterols have a double bond, typically between C-5 and C-6 of the sterol moiety, whereas this bond is saturated in phytostanols
Classification
Phytosterols has two classes
-Sterols
-Stanols (Saturated)
sitosterol campesterol
sitostanol campestanol
DIFFERENTIATION
PHYTOSTEROL CHOLESTEROL
Synthesized by plants only Synthesized by liver(from AcetylCoA) and it is also a essential component of cell membrane
Helps plant to maintain the temperature Helps in maintaining membrane permeability and fluidity
Helps to lower the Low Density Lipo-protien(LDL) in the blood( by 10%)
Serves as a precursor for the biosynthesis of steroid hormones, bile acids, and vitamin D.
Daily intake should be 1-2gm for beneficial effects
Less than 200mg through diet since body also synthesize it.
Dietary phytosterols
The richest naturally occurring sources of phytosterols are vegetable oils and products made from them.
They can be present in the free form and as esters of fatty acid/cinnamic acid or as glycosides, respectively.
The bound form is usually hydrolyzed in the small intestines by pancreatic enzymes.
Phytosterol food sources
Total phytosterol content (mg/100g)
Oils
Rice bran 1055
Corn 952
Wheat germ 553
Flax seed 338
Cottonseed 327
Soybean 221
Peanut 206
Olive 176
Coconut 91
Palm 49
Vegetables (mg /100g)
Beet root 25
Brussels sprout 24
Cauliflower 18
Onion 15
Carrot 12
Cabbage 11
Yam 10
Fruits (mg/100g)
Orange 24
Banana 16
Apple 12
Cherry 12
Peach 10
Pear 8
Nuts (mg/ 100g)
Cashew 158
Almond 143
Pecan 108
Pistachio 108
Walnut 108
Legumes (mg/100g)
Pea 135
Kidney bean 127
Broad bean 124
The intake of naturally occurring phytosterols ranges between 150–450 mg/day
Extraction of PhytosterolsPHASE 1
1 •Deodorization lead in removal of volatiles and gives us a distillate that is rich in free fatty acids, but also significant levels of tocopherols (5-15%) and phytosterols (8-20%).
2 •Transesterification is carried out to give fatty acid methyl esters , and we get free tocopherols and free phytosterols which are separated by distillation.
3 •The free tocopherols are separated from free phytosterols by solvent crystallization and filtration
EXTRACTION: VEGETABLE OIL
4• Addition of MeOH:H2SO4
(50-80degC)• Vigorous stirring for 2-6hrs
5• Vaccum distillation of
methanol• Separation of H2SO4 by hot
water washing
6• Crystallization at 0-25degC
for 1-10hrs• Filtration then washing with
hexane at 10deg• Pure crystals of sterols
Publication number US7632530 B2
Publication type Grant
Application number US
11/373,933
Publication date 15 Aug 2009
Filing date 13 Mar 2006
Priority date 8 April 2005
A process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils.The yield of phytosterols obtained is in the range of 80-90% of free sterols available in with 95-99% purity.
Production of phytostanols from phytosterols
• Phtosterols
(Pd/Pt at High pressure)
Phytostanols
Mechanism
-https://www.youtube.com/watch?v=p-ruFt-s_Bk
Mechanism of action2) Phytosterol precipitates
marginally soluble cholesterol into non absorbable form in the intestine.
1)Reduces the absorption of cholesterol by competing with cholesterol for incorporation into the bile salts micelles or for uptaking of cholesterol by enterocytes.
3) Phytosterols enhance cholesterol excretion back into the intestinal lumen through the ABC G5/G8 transporters
4)They could also prevent esterification of free cholesterol.
Other functions
PHYTOSTEROLS
4)reducing blood sugar level and inflammation
1) Anticarcinogenic properties2) Immune regulation
3)Reducing prostate enlargement
Disease Method Result
Cardio vascular disease
Involved persons with a family history of hypercholesterolemia, consuming spreads fortified with 1.8 to 2.8 grams of sterols/stanols per day over a period of 1-3 months
lowered total cholesterol by seven to 11 percent.
Enlarged prostate Includes a six-month study of 200 men showing symptoms of Enlarged prostate were given 60 mg/d of beta-sitosterol .
Increased peak urinary flow and decreased post-void residual urine volume compared to placebo.
rheumatoid arthritis 2mg of sterols/day for 3 months helping to decrease inflammation and calm the overactive immune response
Control blood sugar in diabetes
2mg of sterols/day for 6-8 months
sitosterol has been shown to normalize blood sugar and insulin levels in Type II diabetics by stimulating the release of insulin in the presence of non-stimulatory glucose concentrations, and inhibiting glucose-6-phosphatase.
Enrichment of food products with phytosterols• Increased interest in phytosterols after canola oil- based
maragrine, enriched by sitostenol ester was introduced comercially in Finland in 1995.
• Nowadays cream cheese, salad dressing and yoghurt are also used.
• They can also be incorporated into baked products, fruit juice, ice cream, and other vehicles.
• They are also being sold with other functional ingredients such as fiber, healthy oils non- absorbable diacylglycerol, almonds, soy protein and minerals.
Stability during processing Under harsh conditions (high temperature i.e. >100˚C and in the presence of oxygen) oxidation of phytosterols can take place.
Microbiologically inert.
Stable over wide pH range.
Food product
3.4gm of Phytostanol ester content in
( FAO REGULATION)
Spread 4 Slices
Yoghurt drink
100 ml
Milk drink 250 ml
Rye bread 4 slices
Capsules 6capsules
Snack Bar 64 gm
Dressing 30 ml In December 2004 the EU Commission published Regulation 608/2004/EC concerning the labelling of foods “with added plant sterols/plant stanols”
Manufacturer Brand name
Raisio Plc. Benecol ®
Cognis Vegapure ®
Lipofoods Lipophytol TM
Enzymotech Ltd. CardiaBeat TM
Pharmaconsult Oy Ltd Multibene ®
Conditions and restrictions of use The Panel recommends that the products to which phytosterols are added
should be consumed only by people who want to lower their blood cholesterol.
The scientific justification of the claim is related to a daily intake of 2 g of plant sterols added to fat-based food formats and low-fat foods such as milk and yoghurt.
Other food forms should be evaluated for their cholesterol-lowering effect.
Should follow labelling regulations issued by EU in Dec’04
DRUG INTERACTIONS
POSITIVE NEGATIVE
It shows synergistic action with statin therapy drugs ( CHOLESTROL LOWERING)
Consumption of plant sterol- and stanol-enriched foods at doses of 1.5 g/d or more have not been found to have adverse effects on fat-soluble vitamin status in well-nourished populations.
Hence resulting in an additional 7-11% reduction in LDL cholesterol.
A number of studies have observed 10-20% reductions in plasma carotenoids after short-term and long-term consumption of plant sterol- or stanol-enriched foods
Adverse effects
The product may not be nutritionally appropriate for pregnant and breastfeeding women and children under the age of five years; patients on cholesterol lowering medication should only consume the product under medical supervision.
Alteration in testosterone metabolism by inhibiting 5-alpha reductase which converts testosterone to dihydrotestosterone.
High doses could potentially affect signal transduction or membrane bound enzyme activity in the cells.
Nausea, indigestion, constipation, diarrhea, etc.
Recent news and research
Phytosterols to be added in dark chocolates to produce a cholesterol-cutting product capable of an FDA health claim
Plant sterols can prevent Alzheimer’s disease
Those who have asthma or other respiratory diseases should be careful, because many supplements are made from soy, can trigger outbreaks.
Phtosterols enriched Peanut butter - Dr. Atif Awad ( State University of New York at Buffalo )
Bunge’s Award winning Functional food – Phtosterol ( phtobake) in microwave pizzas
REFERENCES
• Cholesterol-lowering phytosterols: factors affecting their use and efficacy by Timothy P Carr ,Mark M Ash ,Andrew W Brown (26th july 2010).
• Phytosterols as functional food Components and Nutraceuticals – Paresh C.Dutta
• Phytosterols, Phytostanols And Their Esters -chemical and technical assessment by Richard Cantrill, Ph.D., reviewed by Yoko Kawamura, Ph.D., for the 69th JECFA1
• Mechanisms of decreased cholesterol absorption mediated by phytosterols in the intestinal lumen-Andrew W. Brown University of Nebraska-Lincoln,(1-1-2011)
Reference
http://www.raysahelian.com/phytosterols.html
http://www.fao.org/fileadmin/templates/agns/pdf/jecfa/cta/69/Phytosterols.pdf
http://www.efsa.europa.eu/en/efsajournal/doc/nda_op_ej781_art14_004_plant_sterols-and_blood_cholesterol.pdf?ssbinary=true
https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0CDMQFjAC&url=http%3A%2F%2Fwww.ifst.org%2Fdocument.aspx%3Fid%3D1481&ei=7gdGU5K-HsGHrgeepYDgAw&usg=AFQjCNHtlRihogD3cZ4nab-FjeVxyTkT1g&bvm=bv.64507335,d.bmk