Physical Optics - uni-jena.deOptics... · Bose-Einstein mean photon number thermal light source ->...
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www.iap.uni-jena.de
Physical Optics
Lecture Summer Semester 2018: Photon Optics
2018-05-09
Michael Kempe
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Physical Optics: Content
2
No Date Subject Ref Detailed Content
1 11.04. Wave optics GComplex fields, wave equation, k-vectors, interference, light propagation,
interferometry
2 18.04. Diffraction GSlit, grating, diffraction integral, diffraction in optical systems, point spread
function, aberrations
3 25.04. Fourier optics GPlane wave expansion, resolution, image formation, transfer function,
phase imaging
4 02.05.Quality criteria and
resolutionG
Rayleigh and Marechal criteria, Strehl ratio, coherence effects, two-point
resolution, criteria, contrast, axial resolution, CTF
5 09.05. Photon optics KEnergy, momentum, time-energy uncertainty, photon statistics,
fluorescence, Jablonski diagram, lifetime, quantum yield, FRET
6 16.05. Coherence KTemporal and spatial coherence, Young setup, propagation of coherence,
speckle, OCT-principle
7 23.05. Polarization GIntroduction, Jones formalism, Fresnel formulas, birefringence,
components
8 30.05. Laser KAtomic transitions, principle, resonators, modes, laser types, Q-switch,
pulses, power
9 06.06. Nonlinear optics KBasics of nonlinear optics, optical susceptibility, 2nd and 3rd order effects,
CARS microscopy, 2 photon imaging
10 13.06. PSF engineering GApodization, superresolution, extended depth of focus, particle trapping,
confocal PSF
11 20.06. Scattering LIntroduction, surface scattering in systems, volume scattering models,
calculation schemes, tissue models, Mie Scattering
12 27.06. Gaussian beams G Basic description, propagation through optical systems, aberrations
13 04.07. Generalized beams GLaguerre-Gaussian beams, phase singularities, Bessel beams, Airy
beams, applications in superresolution microscopy
14 11.07. Miscellaneous G Coatings, diffractive optics, fibers
K = Kempe G = Gross L = Lu
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Wave-Particle Duality
• With Maxwell’s theory the description of light as electro-magnetic wave was
seemingly complete, explaining phenomena as refraction, diffraction, and interference
• Around 1900 experimental data emerged that couldn’t be explained by the wave
theory of light: black body radiation and the photo-electric effect
• Electron kinetic energy independent of intensity of light but linearly dependent on light
frequency light as particle stream with energy 𝐸 = ℎ𝑓 with Planck’s constant
(Einstein 1905)
AU
AnodePhoto-
cathode
e-
- +
electron
kinetic energy
light frequency
Evacuated tube
e-
e-
Ref.: Wikipedia
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Photon Properties
Energy of a photon
Planck’s constant
Momentum of a photon
• stable elementary particle with no
charge, no mass
• travels at speed of light in vacuum
c0 and n-times slower in medium
with index of refraction n Particle Wave
𝑐0 = 𝜆0 ∙ 𝑓Speed of photon
𝑐 =𝑐0𝑛=𝜆0𝑛∙ 𝑓 = 𝜆 ∙ 𝑓
𝑘
f, λE,c
𝑝
𝑐0 = 299 792 458 𝑚 / 𝑠
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mean photon flux density
mean photon flux
optical intensity
optical power
optical energy mean number of photons
Energy of photon @ λ=550nm 3.61 ∙ 10−19𝑊𝑠
mean photon flux density
phot/(cm2 s)
Photon Properties
Sun at Earth: 1000W/m² 3.61 ∙ 1018𝑝ℎ𝑜𝑡/(𝑐𝑚2𝑠)
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Uncertainty Relations
• The wave character of the photon is also visible in the fundamental
uncertainty to measure certain quantities simultaneously
• a single-valued k-vector/momentum infinitely extended wave in space
a single-valued frequency/energy infinitely extended wave in time
Gaussian beam:𝑓 𝑥 =
1
2𝜋𝜎𝑥exp −
𝑥2
2𝜎𝑥²𝑓 𝑘 = exp −
𝑘2
2𝜎𝑘²
With 𝜎𝑥 =1
2𝜎𝑘
𝜎𝑥 ∙ 𝜎𝑘 =1
2 Smallest
possible
values𝜎𝑡 ∙ 𝜎𝜔 =1
2
Space-bandwidth product
Duration-bandwidth product
𝐸 = ℏ𝜔 𝜎𝑡 ∙ 𝜎𝐸 ≥ℏ
2
𝑝 = ℏ𝑘 𝜎𝑥 ∙ 𝜎𝑝 ≥ℏ
2
uncertainty of E during measurement time 𝜎𝑡
uncertainty of momentum at measurement
position range 𝜎𝑥
2𝜎𝑘 2𝑙𝑛2
k x
1
𝜎𝑘2𝑙𝑛2
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polarisation properties governed by photon spin angular momentum
photon: spin ±1 (quantum number)
spin angular momentum
Polarization
By E-karimi - Own work, CC BY-SA 3.0,
https://commons.wikimedia.org/w/index.p
hp?curid=16697550
Arbitrary polarization state
Example: linear polarization
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Experiments define the properties
• photons are indivisible (can’t be split )
• but they can still interfere!
2
1
P=50%
P=50%
2
1
P=50%
P=50%
50% detection probability at 1 and 2 0% detection probability at 1 and
100% detection probability at 2
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Light Intensity and Probability Distribution
transmittance
reflectanceprobability intensity
Diffraction and Interference pattern
Beamsplitter:
Source: David Malkahttp://www.ing.iac.es/engineering/CCDgroup.html
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the time of arrival and position
of a single photon is
probabilistic
probability distribution: Poisson
mean photon number
n = 0, 1, 2, … photon number
coherent light source -> arrival of photons statistically independent (at given
energy in one mode)
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Photon Statistics: Coherent Light
n=1
n=3
n=5
n=7
n=9
𝑝 𝑛 = 𝑒− 𝑛 𝑛𝑛
𝑛!
p(n
)
n
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the time of arrival and position
of a single photon is
probabilistic
probability distribution:
Bose-Einstein
mean photon number
thermal light source -> arrival of photons not statistically independent (governed
by energy distribution in one mode at an equilibrium temperature)
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Photon Statistics: Thermal Light
𝑝 𝑛 =1
𝑛 + 1
𝑛
𝑛 + 1
𝑛
𝑛 = expℎ𝜈
𝑘𝐵𝑇− 1
−1
0 5 10 15 200,00
0,05
0,10
0,15
0,20
0,25
n=1
n=3
n=5
n=10
n=50
p(n
)
n
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Photon Statistics
variance
coherent thermal
Poisson Bose-Einstein
standard deviation
mean value
signal-to-noise ratio
𝑝 𝑛 =1
𝑛 + 1
𝑛
𝑛 + 1
𝑛
𝜎𝑛2 = 𝑛 + 𝑛2
𝜎𝑛 = 𝑛 + 𝑛2
𝑆𝑁𝑅 = 𝑛
𝜎𝑛𝑆𝑁𝑅 = 𝑛 𝑆𝑁𝑅 =
𝑛
𝑛 + 𝑛2=
𝑛
𝑛 + 1
𝑝 𝑛 = 𝑒− 𝑛 𝑛𝑛
𝑛!
𝜎𝑛 = 𝑛
𝜎𝑛2 =
𝑛=0
∞
𝑛 − 𝑛 2𝑝(𝑛) 𝜎𝑛2 = 𝑛
𝐸 𝑋 =
𝑛=0
∞
𝑛 ∙ 𝑝(𝑛)
Extreme cases (single mode):
𝐸 𝑋 = 𝑛
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Photon Statistics of Single Modes
variance
coherent partially coherent thermal
Poisson Mandel Bose-Einstein
signal-to-
noise ratio
𝑝 𝑛 =1
𝑛 + 1
𝑛
𝑛 + 1
𝑛
𝜎𝑛2 = 𝑛 + 𝑛2
𝑆𝑁𝑅 = 𝑛 𝑆𝑁𝑅 = 𝑛
𝑛 + 1
𝑝 𝑛 = 𝑒− 𝑛 𝑛𝑛
𝑛!
𝜎𝑛2 = 𝑛
𝑝 𝑛 = 0
∞
𝑒−𝒲𝒲𝑛
𝑛!𝑝 𝒲 𝑑𝒲
mean value 𝑛 𝑛 𝑛 = 𝒲
𝜎𝑛2 = 𝑛 + 𝜎𝒲
2
𝑆𝑁𝑅 = 𝑛
𝜎𝒲2
𝑛 + 1
P is constant
𝜎𝒲2 = 𝑛2
P fluctuates with distribution
𝒲 =1
ℎ𝜈 0
𝑇
𝑃 𝑡 𝑑𝑡
𝑝(𝒲)
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energy conservation:
spontaneous emission stimulated emissionabsorption
atomic energy level differences typically lie in the optical region
Photon-Matter Interactions
𝑃𝑠𝑝 =𝑐
𝑉𝜎 𝜈𝑃𝑎𝑏𝑠 = 𝑛
𝑐
𝑉𝜎(𝜈) 𝑃𝑠𝑡 = 𝑛
𝑐
𝑉𝜎(𝜈)
Probability densities:
𝜎(𝜈): transition cross section
absorbing one photon
from a mode with n photons
emitting one photon
into a mode
emitting one photon
in a mode with n photons
𝑛𝑐
𝑉= 𝜙(𝜈) for monochromatic wave
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molecules vibrate and rotate -> electronic, vibrational and rotational transitions
bending© Tiago Becerra Paolini
asymmetric
stretching
symmetric stretching
Energy Levels of Molecules
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light absorption 10-15 s
internal conversion 10-12 s
fluorescence emission 10-9 s
thermal relaxation 10-12 s
Jablonski Diagram
Transition times
Not every absorbed photon results in fluorescence (fraction = quantum yield)
-> collisional quenching, fluorescence resonance energy transfer, intersystem crossing
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luminescence
fluorescence phosphorescence
rapid emission of a photon: 108 s-1
fluorescence lifetime 10ns or shorter
light emission from excited triplet states
(spin forbidden)
slow emission rates: 103 - 1 s-1
phosphorescence lifetime ms - s or longer
Light Emission from Molecules
Spontaneous emission of light in all modes: 𝑃𝑠𝑝 = 1/𝜏 (spontaneous lifetime)
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• fluorescent minerals
• shelly fish: Aequorea victoria
• stained:
bovine pulmonary artery endothelial cells
Fluorescence
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energy of absorption is
larger than the energy of
emission
Absorption
Stokes
shift Δλ
Emission
Fluorescence Stokes Shift
∆𝐸
typical cross section for absorption: 𝜎 ≈ 10−16𝑐𝑚²
𝐼(𝑧) = 𝐼0 exp(−𝜎 ∙ 𝑁 ∙ 𝑧)
N: molecular concentration
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• quantum yield Q = number of emitted photons / number of absorbed photons
Q=1 -> brightest
emissive rate (photons/s)
rate of non-radiative decay
• lifetime of excited state =
average time the molecule occupies the excited state before returning to the
ground state
single exponential decay
Fluorescence Parameters
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=> decrease in fluorescence intensity
• non-radiative energy transfer
(FRET)
• collisional (dynamic) quenching
(chemical quenchers: oxygen, halogens,
amines)
• static quenching = contact quenching
Fluorescence Quenching
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photoselective excitation of fluorophores by polarised light
transition dipole moment [Cm]
- defined orientation with respect to molecular axis = polarisation of
transition
electric dipole moment
Fluorescence Emission
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Fluorescence Emission
=> rotational diffusion decreases anisotropy (less polarised fluorescent emission)
fixed moleculemolecule rotating infinitely fast
fluorescence anisotropy …intrinsic anisotropy
…fluorescence lifetime
…rotational correlation time
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Fluorescence Lifetime Imaging (FLIM)
Scientific Reports
ISSN 2045-2322 (online)http://www.imperial.ac.uk/photonics/research/
Most often used: Time-correlated
Single-Photon-Counting (TCSPC)Can be utilized for imaging in confocal microscopy
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Measuring diffusion of molecules via
single photon detection in confocal microscopy
fluorescence photon counts
autocorrelation function
translational
diffusion
rotational diffusion
Fluorescence Correlation Spectroscopy (FCS)
𝛿𝐼 𝑡 = 𝐼 𝑡 − 𝐼(𝑡)
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D … diffusion coefficient
N … average number of molecules
… diffusion time
Fluorescence Correlation Spectroscopy (FCS)
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•non-radiative energy transfer
•no intermediate photon
Förster Resonance Energy Transfer (FRET)
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energy transfer efficiencyrate of energy transfer
... Förster distance … donor lifetime in absence of FRET
… donor-acceptor distance
Förster Resonance Energy Transfer (FRET)