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CURRICULUM VITAE

Personal Information:

Name: Nahla Osman Mohamed Ali

Position: Associate Professor

Place and date of Birth: Atbara, Sudan, 1968.

Marital Status: Married with 3 children

Specialization: Molecular Parasitology

Languages: Arabic (Mother Tongue) - English (Proficient).

Work address: Department of Parasitology

Faculty of Veterinary Medicine

University of Khartoum

P. O. Box 32 Shambat Campus

Khartoum North 13314

SUDAN

Phone: (+) 249 -9155 661 142

Fax: (+) 249 -185- 312638

Mobile (1): (+) 249-9121-67481

Mobile (2) : (+) 249-9121- 54004

E-mail: dr_nahla2004@yahoo.com

nahla@uofk.edu

nahlali@hotmail.com

Web site: http://uofk.edu/faculties/veterinary/index.htm

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Academic Background:

Ph. D: Wellcome Centre for Molecular Parasitology, University of Glasgow, UK &

University of Khartoum March. 2003.

Thesis Title: “An Investigation of CRK protein kinases of Leishmania and the assessment of

their potential as drug targets”.

M. Sc.: Wellcome Centre for Molecular Parasitology, University of Glasgow and Faculty of

Veterinary Science, University of Khartoum, Sudan Dec. 1998.

Thesis Title: "Molecular Features of the cell cycle of the parasitic protozoan Leishmania

donovani".

BSc. (Honors) Second Class Division ONE in Zoology: Faculty of Science, University of

Khartoum, Khartoum, Sudan. 1993.

Dissertation Title: "Acute Toxicity of some plant extracts to larvae of Mosquito Culex

quinquefascitus".

Field of Work:

[1]. Molecular investigation of parasitic molecules as possible drug targets (Leishmanial

CDKs and Plasmodial HGPRT).

[2]. Study of the genetic relationships between parasite isolates (Trypanosoma evansi).

[3]. Investigation of Natural products as a possible anti-mosquitocidial agents.

[4]. Proteomic Analysis of Plasmodium spp.

[5]. Molecular characterization of Mosquitoes and investigating their role as vectors.

[6]. Molecular characterization of Sandflies and investigating their role as vectors.

Research Interests:

[1]. Bioinformatics and applied genomics for identifying drugs targets.

[2]. Genomics of parasites and vectors.

[3]. Proteomics of parasites.

[4]. Investigation the role of mosquitoes in pathogens transmission.

Research Background:

[1]. February 1993 - June 1993: Volunteer at the Natural History Museum (University of

Khartoum), Khartoum, Sudan.

Undertaken Research: Working in the Bird Identification, learning some identification

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criteria applied to aquatic birds. During that period I contributed in a Census of the White

stork migrating from north Europe (Germany) to south, Khartoum (Sudan).

Head of Group: Dr. Fathi M. El Raaba .

[2]. March 1997 - September 1997: Post-Graduate (M. Sc.) visiting student, at the

Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, UK (funded

by the WCMP and Gordon Memorial Trust).

Undertaken Research: Working at molecular level on the Leishmania cell cycle putative

genes, the cdc2-related protein kinase 3 (CRK3) and sequencing of the full length gene from a

Sudanese strain of Leishmania donovani and genetic analysis of the identified homologue.

Head of Group: Prof. Jeremy C. Mottram.

[3]. August 1996 -April 1999: Part-time Research Assistant, at the Institute of Endemic

Diseases, Department of Genetics and Molecular Biology, University of Khartoum, Sudan

(funded by WHO/TDR).

Undertaken Research: Working in a confidential project, involving studies at

epidemiological and molecular levels on genetic polymorphism of Leishmania spp. parasite in

different regions of SUDAN.

Head of Group: Prof. Muntaser E. Ibrahim.

[4]. May 1999 - March 2002: Post-Graduate (Ph. D) visiting student, at the Wellcome Centre

for Molecular Parasitology, University of Glasgow, Glasgow, UK (funded by

UNDP/WORLD BANK/WHO Special Program for Research and Training in Tropical

diseases (TDR).

Undertaken Research: Working at molecular level on the Leishmania mexicana;

biochemical analysis of two of the cell cycle regulators; the kinases (CRK1 and CRK3) and

the cyclin (CYCb), their identification, and expression in the prokaryotic system, purification

and investigation of their potentiality as drug targets.

Head of Group: Prof. Jeremy C. Mottram.

[5]. April 2003 - April 2004: Post-doctorate researcher, at Department of Medicine,

Pharmacology, & Toxicology, Faculty of Veterinary Medicine, University of Khartoum.

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(funded by Ministry of Science & Technology (MOST).

Undertaken Research: Working in Molecular Diagnosis of parasitic, viral & bacterial

diseases of domestic animals, using the RT-PCR, nested-PCR and Multiplex-PCR techniques.

Head of Group: Prof. Imadeldin E. Aradaib.

[6]. March 2011 – September 2011: Post-doctorate researcher, at Molecular Biology and

Genetics Unit, Jawaharlal Nehru Centre for advanced Scientific Research (JNCASR),

Bangalore- India. (Funded by CV Raman International Post-doctoral fellowship for African

Researchers).

Undertaken Research: Working in Molecular Characterization of HGPRT from Plasmodium

berghei and Secretome analysis of Plasmodium falciparum (proteoamic approach).

Head of Group: Prof. Hemalatha Balarm. India

[7]. March 2016 – May 2016: Scholarship from Centre for International Cooperation and

Development (CICOPS) at the Department of Biology and Biotechnology University of

Pavia, Italy.

Undertaken Research: Working on different aspects of population structure of Sudanese

strain of Aedes aegypti and microsatellite analysis.

Head of Group: Prof. Guliano Gasperi, Pavia University, Italy.

[8]. June 2016 – August 2016: Fellowship from Women for Africa Foundation at the

Laboratory of Arbovirus and Transmissible Imported Diseases, Carlos III Health Institute,

Madrid, Spain.

Undertaken Research: Detection of arbovirus in samples of vectors through molecular

methods and viral culture.

Head of Group: Dr. Mari Paz Sánchez-Seco, Madrid, Spain.

[9]. April 2017 – August 2017: Fellowship from Women for Africa Foundation at the

Medical Entomology, Carlos III Health Institute, Madrid, Spain.

Undertaken Research: Morphological and Molecular study on Sandflies fauna from Central

and Eastern Sudan.

Head of Group: Dr. Ricardo Molina, Madrid, Spain.

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Employment and positions held:

1993 Graduation from Department of Zoology, Faculty of Science,

University of Khartoum.

1994 Appointment as Teaching assistant at the Department of

Parasitology, Faculty of Veterinary Medicine, University of

Khartoum.

1998 Promotion to Lecturer at the Department of Parasitology,

Faculty of Veterinary Medicine, University of Khartoum.

2003 Promotion to Assistant Professor at the Department of

Parasitology, Faculty of Veterinary Medicine, University of

Khartoum.

April 2003 – To date Member of the Parasitology Departmental Board, Faculty of

Veterinary Medicine, University of Khartoum.

Aug 2003 –March 2005 Appointment as Secretary of the Parasitology Departmental

Board.

Nov 2003 – Aug 2004 Appointment as Academic Coordinator for the subjects of First

year students, Faculty of Veterinary Medicine, University of

Khartoum (This position equivalent to Head Department).

Aug 2003 – To date Member of The Examiners Faculty Board, Faculty of

Veterinary Medicine, University of Khartoum.

Nov 2003 – Aug 2004 Member of the Faculty Board, Faculty of Veterinary Medicine,

University of Khartoum.

Jan 2004 – Aug 2004 Member of the Faculty Examination Board, Faculty of

Veterinary Medicine, University of Khartoum.

3rd

- 15th

March 2005 Supervision of 3rd

year Vet. Students Annual Scientific Tour to

Sennar State.

March 2006- To date Academic Supervisor for undergraduates (1st – 5

th) Vet. Students.

January 2012 – To date Member of Faculty Committee of Promotion of Scientific

Research.

January 2011 Promotion to Associate Professor at the Department of Parasitology,

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Faculty of Veterinary Medicine, University of Khartoum.

March - Oct. 2011 Post doctoral fellowship. Molecular Biology and Genetics Unit,

Jawaharlal Nehru Centre for advanced Scientific Research

(JNCASR), Bangalore- India.

January 2011 – To date Associate Professor at the Department of Parasitology, Faculty

of Veterinary Medicine, University of Khartoum.

New Establishments & Capacity Building:-

[1]. A laboratory for teaching practical General Zoology for undergrad students with:

Dissecting tools for conducting the comparative anatomy work of invertebrates and

vertebrates and all relevant microscopic slides and specimens for Protozoa, Coelenterates,

Helminthes, Annelids and Arthropods.

[2]. A Molecular Biology laboratory for post-grad students with: Cell culture facilities:

–20 oC freezer, cold incubator, reagents and consumables for cell and parasite culture and

training. pH meter, magnetic stirrer, sensitive balance, Horizontal and Vertical Gel

electrophoresis system, Microcentifuge and pipettes and consumables for conducting DNA

extraction – PCR amplification – Gene Cloning - analysis of proteins on SDS- PAGE –

Western blot analysis – cytogenetic analysis.

Contribution to Promotion of Teaching:-

[1]. Zoology:

I have made the current curriculum and all the development and improvement of both the

theoretical and practical sections. This module was used to be conducted in Arabic, but since

the academic year 2015-2016, it has been conducted in English.

I have trained several academic staff currently appointed in different faculties and colleges all

over the country in the field of Zoology.

[2]. Biochemistry of Parasites & Molecular Parasitology:

I played an active role in planning and developing new two curricula for the post-grad

students of Master in Parasitology (by courses & complementary research): Biochemistry of

Parasites (Para – 605) and Molecular Parasitology (Para – 606) are models in our

postgraduate program for its innovative use of demonstrations.

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Awards:

Research grants:

[1]. Training grant for post-graduate (PhD) study from World Health Organization

UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases

(TDR). Switzerland (1999).

[2]. The Arab-British Chamber Charitable Foundation. UK (2000).

[3]. British Federation of women Graduates (BFWG) Post-graduate grant. UK (2000).

[4]. Miss C A M Sym’s Charitable Trust Post-graduate grant. Scotland (2001).

[5]. Gordon Memorial Trust Post-graduate grant. UK (2001).

[6]. Ministry of Higher Education & Scientific Research (MHESR). Sudan (2005)

[7]. University of Khartoum (Directorate for Scientific Research and Cultural Relations).

Sudan (2010).

[8]. CV Raman International Post-doctoral fellowship for African Researchers. India (2011).

[9]. Ministry of Higher Education & Scientific Research (MHESR). Sudan (2013).

[10]. Centre for International Cooperation and Development (CICOPS) Scholarship

University of Pavia, Italy (2016).

[11]. Science by Women - Programme For Women, Science, Technology and Innovation in

Africa Foundation Fellowship, Carlos III Health Institute, Madrid, Spain (2016-2017).

[12]. Ministry of Higher Education & Scientific Research (MHESR). Sudan (2017).

Supervision of Post-graduates:

[1]. Name: Hamid Ibrahim Mohamed Nour Croof

Title of research: “Molecular Diagnosis of Trypanosoma evansi infection in Camels from

Eastern & Western regions of Sudan” –

Degree: M. V. Sc. (Parasitology).

[2]. Name: Hamid Ibrahim Mohamed Nour Croof

Title of research: “Molecular Studies on Camel Trypanosomosis from different regions of

Sudan”

Degree: Ph. D. (Molecular Parasitology).

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[3]. Name: Mai Mohamed Al Nour

Title of research: “Molecular Identification of Anopheles spp. and Genotyping of Plasmodium

falciparum in Kosti region of the Central Sudan”

Degree: M. Sc. (Medical Entomology).

Examination of Post-graduates (MSc. / MV. Sc.):

[1]. Name: Ghada Ali Homeida Ali

Title of research: “Trypanosoma evansi in naturally infected Camels and experimental

animals” - May 2007

[2]. Name: Hamid Ibrahim Mohamed Nour Croof

Title of research: “Molecular Diagnosis of Trypanosoma evansi infection in Camels from

Eastern & Western regions of Sudan” - January 2008.

[3]. Name: Nadia Edris Mohamed Salih

Title of research: “Infectivity and Serological Studies on Trypanosoma evansi isolates” -

March 2011.

Examination of theses:

[1]. Title of thesis: “Investigation of Drug Resistance in Leishmania donovani isolate from

Eastern Sudan”

Degree: Ph. D, U of Al-Gazeira - 2003

[2]. Title of thesis: “Molecular diagnosis of Toxoplasma gondii”

Degree: M. Sc., U of Al-Gazeira - 2006

[3]. Title of thesis: “Trypanosoma evansi in naturally infected Camels and experimental

animals”

Degree: M. Sc. Dept of Biology Faculty of Education U of K. - May 2007.

[4]. Title of thesis: “Molecular Characterization of Dermotropic Leishmania parasite”

Degree: M. Sc. Dept of Molecular Biology, Institute of Endemic Diseases, U of K. - June

2007.

[5]. Title of thesis: “Molecular Diagnosis of Trypanosoma evansi infection in Camels from

Eastern & Western regions of Sudan”

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Degree: M. V. Sc. Dept of Parasitology, Faculty of Veterinary Medicine, U of K. - January

2008.

[6]. Title of thesis: “Infectivity and Serological Studies on Trypanosoma evansi isolates”

Degree: M. Sc. Dept of Parasitology, Faculty of Veterinary Medicine, U of K. - March 2011.

Meetings & Conferences:

[1]. the Scientific Conference for Camel Research and Production. Friendship Hall,

Khartoum, 17-18, April 2013. Hamid I. M. N. Croof; Sara A. M. Abbas; Nahla O. M. Ali

“Camel Trypanosomiasis in Eastern and Western regions of Sudan: Molecular Diagnosis,

other Diseases Morbidity and Grazing Conditions”. Poster presentation.

[2]. Afro – Asia workshop on Advanced Topics in Chemistry. TWAS-ROCASA.

Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore – India. June 13-17,

2011.

[3]. 2nd

International Conference on Applied Biotechnology “ICAB-2010”

“Biotechnology for Sustainable Human Society” 25 to 27 October 2010, Friendship Hall,

Khartoum.

[4]. Scientific Conference for Higher Studies College University of Khartoum – Khartoum,

March 2010.

[5]. ISMB/ECCB 2004, International Bioinformatics Meeting – Glasgow, UK. July 31st –

August 4th

2004. "Cyclin-dependent kinases of Leishmania: Sequence Analysis and

Genomics". Accepted for Poster Presentation.

[6]. ICGEB Bioinformatics Workshop. New Delhi India. 20th

-31st October 2003.

“Leishmanial CRKs as drug targets: Identification and Analysis of cyclin-dependent kinases”.

Oral Presentation.

[7]. 3rd

International Conference, Inhibitors of Protein Kinases. Warsaw Poland. 22nd

–

28th

June 2003. “An Investigation of CRK protein kinases of Leishmania and the assessment

of their potential as drug targets”. Poster Presentation.

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[8]. Conference on: ‘Endemic and Infectious Diseases in Sudan’. Khartoum. 5th

-8th

May

2003. “Isolation and characterization of a novel protein kinase from L. donovani donovani:

LdCRK3 shows high homology to both LmmCRK3 & LmajCRK3”. Oral Presentation

[9]. International Centre for Genetic Engineering and Biotechnology (ICGEB). Trieste

Italy 3th

-5th

October 2002. “Leishmania parasite: Analysis of CRK3 protein kinase, CYCa

cyclin, and the inhibitory effect of Flavopiridol”. Oral Presentation.

Theoretical & practical course on ‘Molecular biology of Leishmania’ 3th

-5th

October 2002,

Trieste, Italy.

[10]. 50th

Annual Meeting of American Society of Tropical Medicine and Hygiene

(ASTMH). Atlanta USA 12th

–16th

November 2001. “Identification and analysis of

Leishmania cyclin-dependent kinases”. Accepted for Poster Presentation.

[11]. BFWG London Association Academic Day. London, UK 19th

May 2001. “Molecular

biochemical study on some cell cycle regulators in Leishmania: Kinases & Cyclins”. Oral

Communication.

[12]. British Society of Parasitology (BSP) Spring Meeting. University of Keele, UK 17th

-

20th

April 2001. “Cyclin-dependent kinases of Leishmania”. Poster Presentation.

[13]. Scottish Universities Molecular Parasitology (SUMP). Kindrogan. UK 5th

-7th

May

2000.Cyclin-dependent kinases of Leishmania". Poster Presentation.

[14]. First Scientific Meeting, Institute of Endemic Diseases, Khartoum, SUDAN Feb.

1999. "Isolation and characterization of cdc2-related protein kinase (CRK3) homologue, the

eukaryotic cell cycle regulator from the protozoan Leishmania donovani donovani". Oral

Communication.

Previous Teaching Experiences:

(I) Animal Biology / General Zoology:

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Faculty of Science; Faculty of Agriculture; Faculty of Animal Production, Faculty of

Education - University of Khartoum. Faculty of Veterinary Medicine - University of

Bahr El Gazal (1993 - 1996).

Department of Basic Sciences & Department of Parasitology, Faculty of Veterinary

Sciences, University of Khartoum (1994 - 1999).

Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum

(April 2003- To date).

Arabic Learning Centre, Pretoria, Republic of South Africa (Biology, Chemistry and

Physics) (2004).

(II) Molecular Biology:

Biochemistry of Parasites – (Theory & practical) - Master students -Department of

Parasitology, Faculty of Veterinary Medicine, University of Khartoum (2012 – To

date).

Molecular Parasitology – (Theory & practical) - Master students - Department of

Parasitology, Faculty of Veterinary Medicine, University of Khartoum (2012 – To

date).

Mechanisms of protein secretion in prokaryotic cells – Master students – Department

of Zoology, Faculty of Science, University of Khartoum (2012).

DNA Technology: “DNA extraction and PCR technique- theoretical aspects” -

Master students, Department of Parasitology, Faculty of Veterinary Medicine,

University of Khartoum (2007).

Molecular Cell Biology: “Cell Cycle in Trypanosomatids” Faculty of Science,

University of Khartoum (2006).

Molecular Cell Biology: “Regulation of Cyclin-dependent kinases (CDKs) in

eukaryotic cells” - Faculty of Science, University of Khartoum (2005).

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DNA Replication and Eukaryotic cell cycle - Faculty of Veterinary Medicine,

University of Khartoum (2003 – To date).

DNA Technology: “Molecular Cloning technique” - Academy of Medical Sciences,

Khartoum (1997).

(III) Contribution in Training courses & workshops:-

Taught and demonstrated the “RT-PCR, Multiplex-PCR, and nested-PCR techniques

for detection of RNA virus” in the “Applied Introductory Course on Molecular

Biology” held by Tropical Medicine Research Institute (TMRI), National Centre for

Research (NCR), Ministry of Science & Technology (MOST). Khartoum (24th

May –

3rd

June 2003).

Actively contributed in the design and execution of practical sessions: “DNA cloning -

Sequencing & Sequence analysis - Introduction to Bioinformatics: “Primers

Designing”. Molecular Biology Training course. Faculty of Veterinary Medicine,

University of Khartoum (Feb 2003).

Taught and demonstrated the Molecular Cloning technique in the “Basic & Applied

Course on Molecular Biological techniques in the Diagnosis of Infectious Diseases”.

Khartoum, Sudan (Jan 1998).

(IV) Seminars:-

“Analysis of Cyclin-dependant protein kinases of Leishmania and investigation of

their role as Drug Targets”. Faculty of Science, University of Khartoum (2004).

“CRK protein kinases of Leishmania and assessment of their potential as Drug

Targets”. Faculty of Veterinary Medicine, University of Khartoum (2003).

“CRK protein kinases of Leishmania and assessment of their potential as Drug

Targets”. Institute of Endemic Diseases, University of Khartoum (2003).

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“Leishmanial Protein kinases as Drug Targets”. Faculty of Medicine, University of

Khartoum (2002).

Professional Advanced courses & workshops attended:-

[1]. MS – MALDI – TOF workshop (2011). At Molecular Biology and Genetics Unit,

Jawaharlal Nehru Centre for advanced Scientific Research (JNCASR), Bangalore- India.

[2]. Regional Training Course on Bioinformatics Applied to Tropical diseases In Africa. 2nd

-

15th

February 2004, South African National Bioinformatics Institute, University of the

Western Cape, Cape Town, South Africa sponsored by UNICEF-UNDP-World Bank-WHO

Special Programme for Research and Training in Tropical Diseases (TDR) – National

Bioinformatics Network (NBN) and Medical Research Council (MRC) South Africa.

[3]. ICGEB Bioinformatics Workshop. 20th

– 31st October 2003, New Delhi, India held by

International Centre for Genetic Engineering and Biotechnology (ICGEB) and the Italian

Embassy in India.

[4]. Theoretical & practical course on ‘Molecular biology of Leishmania’ 3rd

-5th

October

2002, Trieste, Italy held by International Centre for Genetic Engineering and Biotechnology

(ICGEB).

[5]. Theoretical & practical course on ‘Cytogenetics and Molecular Cytogenetics of Cancer’

24th

July – 4th

August 2002, Khartoum held by Sudanese Cancer Research Group &

Department of Clinical Genetics, University of Lund, Sweden.

[6]. Training at Wellcome Centre for Molecular Parasitology, University of Glasgow (Part of

my Ph.D). (1999-2002). Glasgow, UK. Funded by WHO/UNDP/World Bank/WHO Special

Program for Research and Training in Tropical Diseases (TDR).

[7]. Advanced Sequence Analysis course, January 2000 – Glasgow University Computing

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Service, Glasgow, UK

[8]. GCG and the Internet course, November 1999 – Glasgow University Computing Service,

Glasgow, UK.

[9]. Introduction to Immunology and Molecular Biology (1997). Institute of Endemic

Diseases, University of Khartoum.

[10]. Training 6 months at Wellcome Centre for Molecular Parasitology, University of

Glasgow (Part of my M.Sc.). (1997), Glasgow, UK. Funded by WCMP and Gordon Memorial

Trust.

[11]. Basic & Applied Course on "Molecular Biology Techniques In Research and Diagnosis

of Infectious Diseases" 7th

-15th

January 1996, Khartoum held by Institute of Tropical

Medicine, National Center for Research & Unit of Genetics and Molecular Parasitology,

National Public Health Laboratory, Sudan.

[12]. Biostatistics, using Statistical Package for Social Studies (SPSS) (1995), Khartoum held

by Institute of Tropical Medicine, National Center for Research & DANIDA (Denmark),

Sudan.

Research projects:

1. Leishmanial Cyclin-dependent kinases as drug targets: Role of inhibition of L. donovani

CRK3 in development of Sudanese visceral leishmaniasis - Principal Investigator.

Funded by: Ministry of Higher Education & Scientific Research (2005 - 2008).

2. Evaluation of a simple PCR-based technique for field diagnosis of Trypanosoma evansi

infection in Camels from Eastern and Western regions of Sudan in comparison to

parasitological techniques and card agglutination test (CATT) - Principal Investigator.

Funded by: Ministry of Animal Resources – Gedariff State (2006 – 2007).

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3. Genetic diversity of Trypanosoma evansi in Sudanese Camels – Principal Investigator.

Funded by: University of Khartoum (2011 – 2013).

4. Molecular and biochemical parasitology: Characterization of hypoxanthine guanine

phosphoribosyl transferase from Plasmodia. Principal Investigator. Funded by: Federation of

Indian Chambers of Commerce and Industry (FICCI), India (2011).

5. Surveillance of mosquitoes in Central Sudan and investigation their role in pathogens

transmission – Principal Investigator. Funded by: Ministry of Higher Education & Scientific

Research (2014 – 2016).

6. Population genetic structure of the Sudanese mosquito Aedes aegypti (Diptera: Culicidae).

Principal Investigator. Funded by: Centre for International Cooperation and Development

(CICOPS), Pavia University, Italy (2016).

7. Surveillance of arboviuses in mosquitos from 3 different regions of Sudan. Principal

Investigator. Funded by: Women for Africa Foundation, Madrid, Spain (2016).

8. Morphological and Molecular study on Sandflies fauna from Central and Eastern Sudan.

Funded by: Women for Africa Foundation, Madrid, Spain (2017).

9. Molecular Survey and Characterization of Mosquitoes in Central Sudan and investigating

their role in transmission of parasites. Funded by: Ministry of Higher Education & Scientific

Research (Jan 2017– Dec 2018).

Societies Memberships:

Sudanese Society for Parasitology Science (SSPS).

British Society for Parasitology (BSP).

International Federation of University Women (IFUW).

British Federation of Women Graduates (BFWG).

International Society for Computational Biology (ISCB).

African Society for Bioinformatics and Computational Biology (ASBCB).

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African Bioinformatics Network (Abionet).

Sudanese Academy of Young Scientists (SAYS).

Experiences & skills:

[I]. Laboratory:

DNA extraction from parasites and different biological materials, PCR, Gel and PCR purification,

Southern blotting, Restriction analysis, DNA cloning, generation of construct. Screening of

cDNA library using radioactive and non-radioactive methods, Sequencing and Site-directed

mutagenesis. Sequence analysis using Vector NTI software. Facs analysis of DNA content

during different phases of the cell cycle. Histone HI kinase assay. Recombinant protein

production & purification on Ni-NTA resin and Bio-rad FLPC machine, Western blot

analysis, Protein analysis and visualization on SDS-PAGE. Culture of: Cell line, Leishmania,

T. evansi and Plasmodium parasites. Identification of mosquitos species. Mass Spectrometry

(MS- MALDI-TOF): Preparation of sample, enzymatic digestion, In-gel digestion, peptide

purification, Protein Identification of Mass spectrometry data using MASCOT software. RT-

PCR, nested – PCR, Real Time – PCR, Virus culturing, SeqMan sequence analysis software.

[II]. Computer:

Fundamentals of computers and networks, MS-DOS, Windows 9x, 2000, XP.

Microsoft Office, Word, LOTUS123, D-BASEIII+, Excel, PowerPoint.

Netscape Communicator, Microsoft Internet Explorer.

SPSS statistical program.

Internet regular user mainly for information search and email.

[III]. Bioinformatics:

Primers designing, Restriction site analysis.

Sequence Analysis and comparison (BLAST – CLUSTALW/X).

Phylogenetic Analysis.

Vector NTI software package.

Molecular Biology Computing; GCG Usage.

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Protein structure prediction.

3D modeling of protein structures.

Genomics: Gene Prediction, Assembly, Annotation, Comparative Genomics.

EST clustering.

Similarity Searches.

Familiar with Plasmodium Data Base, PlasmoDB, MR4.

Familiar with VectorBase Data Base.

Familiar with TriTrypDB, Kinetoplastid Genomic Resources.

MASCOT software for identification of protein sequence.

SeqMan analysis for sequences

Reviewer:

Experimental Parasitology- International Journal –2004 - 2005

Journal of Veterinary Medicine and Animal Health – 2015- To date

University of Khartoum Journal of Veterinary Medicine and Animal Production – 2015 –

To date

Publications:

A. Theses:- (Bsc. MSc. PhD.)

[1]. Ali, N. O. M. (1992). "Acute Toxicity of some plant extracts to larvae of Mosquito Culex

quinquefascitus". BSc. Dissertation, Faculty of Science (Department of Zoology), University

of Khartoum.

[2]. Ali, N. O. M. (1998). Molecular features of the Leishmania donovani cell cycle. MSc.

Thesis, Faculty of Veterinary Science (Department of Parasitology), University of Khartoum.

[3]. Ali, N. O. M. (2003). An investigation of CRK protein kinases of Leishmania and the

assessment of their potential as drug targets. PhD. thesis, Faculty of Veterinary Medicine,

University of Khartoum.

B. Dataset :-

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[1]. Ali, N.O.M. (2001). Leishmania donovani donovani mRNA for cdc2-related kinase 3

(crk3 gene). GenBank: AJ426472.1. gi|18076012|emb|AJ426472.1|LDO426472[18076012].

[2]. Ali, N.O.M. (2002). Leishmania mexicana mexicana partial cyca gene for cyclin.

GenBank: AJ426476.1. gi|18676380|emb|AJ426476.1|LME426476[18676380].

C. Research Articles:-

[1]. Ali, N. O. M.; Ibrahim, M. E.; Aradaib, I. E.; Grant, K. M.; Mottram, J. C. (2003). An

Investigation of CRK protein kinases of Leishmania and the assessment of their potential as

drug targets. Cellular & Molecular Biology Letters, 8 (2A):571-572.

[2]. Ali, N.; Karrar, A. E.; Majid, A. A.; Mohammed, M. H.; and Aradaib, I. E. (2003). RNA

Genome Profiles of Some Sudanese and South African Serotypes of Bluetongue Virus Using

Agarose Gel Electrophoresis and SDS-PAGE. Journal of Animal and Veterinary Advances 2

(6):358-361.

[3]. Aradaib, I. E. and Ali, N. O. M. (2004). Current status and future prospects of epizootic

haemorrhagic disease of deer – a review. Veterinarski Arhiv, 74-(1): 63-83.

[4]. Aradaib, I. E.; Mohammed, M. E.; Sarr, J. A.; Idris, S. H.; Ali, N. O..; Majid, A. A.;

Karrar, A. E. (2006). A Simple and Rapid Method for Detection of African Horse Sickness

Virus Serogroup in Cell Cultures Using RT-PCR. Veterinary Research Communications.

30(3): 319-24. PMID: 16437307 [PubMed - indexed for MEDLINE]

[5]. Gomes, F. C.; Ali, N. O.; Brown, E.; Walker, R. G.; Grant, K. M.; Mottram, J. C. (2010):

Leishmania mexicana cyclin-dependent kinase complex CRK3:CYCA is active in the absence

of T-loop phosphorylation. Molecular and Biochemical Parasitology. 171(2): 89-96.

doi: 10.1016/j.molbiopara.2010.03.002. Epub 2010 Mar 23. PMID: 20338198

[PubMed - indexed for MEDLINE]

[6]. Ali, N. O. M.; Ibrahim, M. E.; Grant, K. M.; & Mottram, J. C. (2010). Molecular

cloning, characterization and overexpression of a novel cyclin from Leishmania mexicana

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mexicana. Pakistan Journal of Biological Sciences 13 (16): 775-784.

PMID: 21850927 [PubMed - indexed for MEDLINE]

[7]. Ali, N. O. M. and El Raaba, F. M. (2010). Larvicidal Activity of Some Plant Extracts to

Larvae of the Mosquito Culex quinquefasciatus (Say 1823). European Review for Medical

and Pharmacological Sciences 14(11): 925-933. PMID: 21284341 [PubMed - indexed for

MEDLINE]

[8]. Ali, N. O. M.; Croof, H. I.; and Abdalla, H. S. (2011). Molecular Diagnosis of

Trypanosoma evansi Infection in Dromedary Camels from Eastern and Western regions of

the Sudan. Emirates Journal of Food and Agriculture 23(4): 320-329.

[9]. Croof, H. I.; Abdalla, H. S. and Ali, N. O. M. (2012). Assessment of Trypanosoma

evansi Infection in Camels herd from Gedariff and Kordofan states. University of Khartoum

Journal of Veterinary Medicine and Animal Production. 3 (1): 3-27.

[10]. Ali, N. O. M.; Ibrahim, M. E; Grant, K. M.; & Mottram, J. C. (2012). Leishmania

mexicana: Expression; characterization & activity assessment of E. coli-expressed

recombinant CRK3. European Review for Medical and Pharmacological Sciences. 16 (10):

1338-1345. PMID: 23104649 [PubMed - indexed for MEDLINE]

[11]. Ali, N. O. M.; Ibrahim, M. E.; Abdalla, H. S.; Mukhtar, M. M.; El-Hassan, A. M. &

Mottram, J. C. (2012). Isolation and characterization of a cdc2-related protein kinase 3

(CRK3) from a Sudanese strain of Leishmania donovani. International Research Journal of

Pharmacy and Pharmacology 2(9): 215-224.

[12]. Ali, N. O. M.; Grant, K. M.; Mottram, J. C. (2013). Expression, Purification, and

Biochemical Characterization of a Recombinant Protein Kinase CRK1 of Leishmania

mexicana. Scholars Journal of Applied Medical Sciences 1(2): 35-40.

[13]. Ali, N. O. M.; Abdella, H. S.; Younis, S. A. (2014). Activity of anti-cancer protein

kinase inhibitors against growth of different stages of Leishmania spp. Scholars Academic

20

Journal of Pharmacy 3(3): 294-300.

[14]. Ali, N. O. M. (2014). Secretome Analysis of Plasmodium falciparum intraerythrocytic

asexual stage using SDS-PAGE electrophoresis. International Journal of Science and

Research 3(6): 351-355.

[15]. Nahla O. M. Ali and Nuha O. M. Ali. (2015). Scorpion Sting in Different Regions of

Sudan: Epidemiological and Clinical Survey among University Students. International

Journal of Bioinformatics and Biomedical Engineering. 1(2): 147-152.

[16]. Nahla O. M. Ali. (2015). Bioinformatic Analysis of Phosphoglucomutase (PGM2) from

Different Species of Plasmodia Using Computational Tools. International Journal of

Bioinformatics and Biomedical Engineering. 1(2): 181-184.

[17]. Nahla O. M. Ali. (2015). Bioinformatical Analysis of HGPRT transferase from

different malaria parasite Plasmodium spp. Using Computational Tools. Malaysian Journal of

Medical and Biological Research. 2 (3): 242-246.

[18]. Nahla O. M. Ali. (2015). Bioinformatic analysis of Plasmodium berghei Hypoxanthine-

guanine-xanthine phosphoribosyltransferase (HGPRT): a potential therapeutic target. Global

Scientific Journal of Molecular Biology 1 (2015): 14-22.

[19]. Nahla O. M. Ali. (2016). Molecular Identification of a cDNA Encoding the

Hypoxanthine-Guaninephosphoribosyltransferase (HGPRT) from Rodent Malaria Strain of

Plasmodium berghei. International Journal of Bioinformatics and Biomedical Engineering.

2(1): 8-14.

[20]. Mai M. Al nour; Mariam A. Aboud; Nahla O. M. Ali. (2016). Morphological

identification, Species Composition and Distribution of mosquitoes in Kosti region, White

Nile State, Central Sudan. Malaysian Journal of Medical and Biological Research. 3 (3): 131-

142.

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[21]. Croof, H., Nour, M., & Ali, N. (2017). Morphological Identification of Horse Flies

(Diptera: Tabanidae) and Estimation of their Seasonal Abundance in Al-Showak District,

Gedaref State, Eastern Sudan. IRA-International Journal of Applied Sciences (ISSN 2455-

4499), 6(2), 41-54. doi:http://dx.doi.org/10.21013/jas.v6.n2.p3

[22]. Hamid I. M. N. Croof, Imna Malelle, Hamis S. Nyingilili, Nahla O. M. Ali. (2017).

Molecular isolation of Trypanosoma evansi from horse flies (Diptera: Tabanidae) from

Eastern Sudan. Global Scientific Journal of Molecular Biology 1(2017): 23-28.

[23]. Hamid I. M. N. Croof, Imna Malelle, Hamis S. Nyingilili, Sonia Sadeq, Darren Brooks,

Nahla O. M. Ali. (2017). First report of Molecular identification of Trypanosoma evansi in

Camelus dromedarius from Nile River and Red Sea states of the Sudan. Global Scientific

Journal of Veterinary 1(2017): 1-7.

[24]. Hamid I. M. N. Croof, Imna Malelle, Darren Brooks, Hamid Suliman Abdella, Nahla O.

M. Ali. (2017). Molecular Isolation and Characterization of Trypanosoma evansi in

Dromedary Camels from Different Regions of Sudan. American Journal of Microbiology and

Biotechnology (2017); 4(6): 67-74.

[25]. Hamid I. M. N. Croof, Mai Mohamed El-Nour, Altayeb Khogali Ahmed Ali, Nahla O.

M. Ali. (2017). Cytogenetic Analysis of the Sudanese Horse Flies (Diptera: Tabanidae) from

Gedaref State, Eastern Sudan. International Journal of Biological Sciences and Applications

2017; 4(5): 56-61.

[26]. Hamid I. M. N. Croof, Imna Malelle, Hamis S. Nyingilili, Sonia Sadeq, Darren Brooks,

Nahla O. M. Ali. (2017). Phylogenetic Analysis of Trypanosoma evansi from Sudan Based

on Ribosomal DNA Sequences. American Journal of Microbiology and Biotechnology 2017;

4(6): 75-82.

D. Meetings Proceedings:-

[1]. Hamid I. M. N. Croof; Sara A. M. Abbas; Nahla O. M. Ali. (2013). “Camel

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Trypanosomosis in Eastern and Western regions of Sudan: Molecular Diagnosis, other

Diseases Morbidity and Grazing Conditions” The Scientific Conference for Camel Research

and Production. Friendship Hall, Khartoum, Sudan.

[2]. Ali, N. O. M.; Grant, K. M.; Mottram, J. C. (2004): “Cyclin-dependent kinases of

Leishmania: Sequence Analysis and Genomics”. ISMB/ECCB International Bioinformatics

Meeting – Glasgow, UK.

[3]. Ali, N. O. M.; Ibrahim, M. E.; Grant, K. M.; Mottram, J. C. (2003): “Leishmanial CRKs

as drug targets: Identification and Analysis of cyclin-dependent kinases”. ICGEB

Bioinformatics Workshop. New Delhi, India.

[4]. Ali, N. O. M.; Ibrahim, M. E.; Aradaib, I. E.; Grant, K. M.; Mottram, J. C. (2003): “An

Investigation of CRK protein kinases of Leishmania and the assessment of their potential as

drug targets”. 3rd

International Conference “Inhibitors of Protein Kinases”. Warsaw, Poland.

[5]. Ali, N. O. M.; Ibrahim, M. E.; Abdella H. S.; Mottram, J. C. (2003): “Isolation and

characterization of a novel protein kinase from L. donovani donovani: LdCRK3 shows high

homology to both LmmCRK3 & LmajCRK3”. Conference on: ‘Endemic and Infectious

Diseases in Sudan”. Khartoum, Sudan.

[6]. Ali, N. O. M.; Ibrahim, M. E.; Grant, K. M.; Mottram, J. C. (2002): “Leishmania

parasite: Analysis of CRK3 protein kinase, CYCa cyclin, and the inhibitory effect of

Flavopiridol”. ICGB Theoretical & practical course on ‘Molecular biology of Leishmania’.

Trieste, Italy.

[7]. Ali, N. O. M.; Grant, K. M.; Mottram, J. C. (2001): “Identification and analysis of

Leishmania cyclin-dependent kinases”. American Society of Tropical Medicine and Hygiene

(ASTMH). Atlanta, USA.

[8]. Ali, N. O. M.; and Mottram, J. C. (2001): “Molecular biochemical study on some cell

cycle regulators in Leishmania: Kinases & Cyclins”. BFWG London Association Academic

Day. . London, UK.

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[9]. Ali, N. O. M.; Grant, K. M.; Mottram, J. C. (2001): “Cyclin-dependent kinases of

Leishmania”. British Society of Parasitology (BSP) Spring Meeting. University of Keele, UK.

[10]. Ali, N. O. M.; Grant, K. M.; Mottram, J. C. (2000): Cyclin-dependent kinases of

Leishmania". Scottish Universities Molecular Parasitology (SUMP). Kindrogan, UK.

[11]. Ali, N. O. M.; Ibrahim, M. E.; Abdella, H. S.; Mottram, J. C. (1999): "Isolation and

characterization of cdc2-related protein kinase (CRK3) homologue, the eukaryotic cell cycle

regulator from the protozoan Leishmania donovani donovani". First Scientific Meeting,

Institute of Endemic Diseases. Khartoum, Sudan.