PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.
-
Upload
christopher-scates -
Category
Documents
-
view
221 -
download
4
Transcript of PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.
![Page 1: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/1.jpg)
PCR PuzzleClass instructions
![Page 2: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/2.jpg)
Start of lesson
• Have the following at front of the class:• Template
![Page 3: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/3.jpg)
Start of lesson continued
• Primers
• Bases
![Page 4: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/4.jpg)
Correct base pairing is critical!
• Green (Guanine) pairs with yellow (Cytosine)
• Blue (Adenine) pairs with orange (Thymine)
![Page 5: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/5.jpg)
The devil is in the detail!
• The 5’ prime and 3’ prime ends of the bases must be round the right way!
![Page 6: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/6.jpg)
Cycle 1 - denature
• Spilt class in 4 pairs or groups of students• Each group will copy one STRAND from the
template• Thus, it is important to think of a template as
two strands rather than one double stranded piece of DNA
![Page 7: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/7.jpg)
Cycle 1 - denature
• Two groups will do the first cycle• Give Group 1 one template strand
• Give Group 2 the other template strand
![Page 8: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/8.jpg)
Students comes to front to get primer
• One student from each group comes to front of class to get primer
![Page 9: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/9.jpg)
Students comes to front to get primer
• If they have double orange in their template, pick one double blue primer
• The other group takes one green-blue primer
![Page 10: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/10.jpg)
Cycle 1 - Anneal primer
• Each group adds primer to the template
• Make sure 5’ to 3’ prime is correct!
![Page 11: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/11.jpg)
Cycle 1 - extend
• Students take bases required to finish strand• And add the bases to complete the copy
![Page 12: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/12.jpg)
Cycle 1 - observations
• When finished filling in blanks, each group brings product to front of class and put on desk or floor
![Page 13: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/13.jpg)
Cycle 1 - observations
• Make observations:– How many copies do you get?– How many are just the target sequence (ie begin
and end with primers)?
![Page 14: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/14.jpg)
Cycle 2 - denature
• Groups 1 to 4 will do Cycle 2• Give each group a strand:
![Page 15: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/15.jpg)
Cycle 2 – Anneal primer• Each group adds appropriate primer to the
template strand
• Make sure 5’ to 3’ prime correct way round!
![Page 16: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/16.jpg)
Cycle 2 - extend
• Students take it in turns to come to front to take matching base to add to template
![Page 17: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/17.jpg)
Cycle 2 - observations
• When finished filling in blanks, each group brings product to front of class and put on desk or floor
![Page 18: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/18.jpg)
Cycle 2 - observations
• Make observations:– How many copies do you get?– How many are just the target sequence (ie begin
and end with primers)?
![Page 19: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/19.jpg)
Cycle 3 - denature• Groups 1 to 4 will do Cycle 3• Give each group two strands:
![Page 20: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/20.jpg)
Cycle 3 – Anneal primer• Each group adds appropriate primer to the
template strand
• Make sure 5’ to 3’ prime correct way round!
![Page 21: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/21.jpg)
Cycle 3 - extend
• Students take it in turns to come to front to take matching base to add to template
![Page 22: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/22.jpg)
Cycle 3 - observations• When finished filling in blanks, each group
brings product to front of class and put on desk or floor
![Page 23: PCR Puzzle Class instructions. Start of lesson Have the following at front of the class: Template.](https://reader036.fdocuments.in/reader036/viewer/2022062404/551bb96a550346b9588b4596/html5/thumbnails/23.jpg)
Cycle 3 - observations
• How many copies do you get?• How many are just the target sequence (ie
begin and end with primers)?• What if anything, has happened to the
template?• How many bases with red, black and white do
you now have?