PCR PCR Polymerase Polymerase

Chain ReactionChain Reaction a tool to generate a a tool to generate a

large amount of a gene large amount of a gene of interestof interest

Polymerase Chain Reaction

synthesizes only the gene of

interest by using Forward and

Reverse primers (specific small

pieces of DNA) with the help of the

enzyme Polymerase. All cycles

follow a strict order of timed

temperatures.

Gene of Interest

Forward primer

First cDNA strand

Reverse primer

Second cDNA strand

Restriction of plasmid

releases the DNA sequence of interest

Plasmid

DNA

DNA-sequence in a plasmid