PCR By: Cody Alveraz Ted Dobbert Morgan Pettit. What is PCR? PCR is a polymerase chain reaction...
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Transcript of PCR By: Cody Alveraz Ted Dobbert Morgan Pettit. What is PCR? PCR is a polymerase chain reaction...
![Page 1: PCR By: Cody Alveraz Ted Dobbert Morgan Pettit. What is PCR? PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of.](https://reader035.fdocuments.in/reader035/viewer/2022062805/5697c0251a28abf838cd51d1/html5/thumbnails/1.jpg)
PCRBy:
Cody Alveraz Ted Dobbert
Morgan Pettit
![Page 2: PCR By: Cody Alveraz Ted Dobbert Morgan Pettit. What is PCR? PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of.](https://reader035.fdocuments.in/reader035/viewer/2022062805/5697c0251a28abf838cd51d1/html5/thumbnails/2.jpg)
What is PCR?
PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences.
It was developed in 1983 by Kary Mullis.
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Procedures of PCR Denaturation: At 201.2 F degrees the
double stranded DNA melts and opens into 2 pieces of single-stranded DNA.
Annealing: At medium temperatures, at around 54 degrees Celsius, the primers pair up with the single-stranded “template”. On the small length of double-stranded DNA, the polymerase attaches and starts copying the template.
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Procedures of PCR (cont.) Extension: AT 72 degrees Celsius, where
the polymerase works best, and DNA building blocks complementary to the template are coupled to the primer, making a double-stranded DNA molecule.
With one cycle a segment of double-stranded DNA template is amplified into two separate pieces of double stranded DNA.
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What is good about PCR? PCR is good technique because its not
expensive, rapid and a good way to get millions of copies of DNA.
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PCR Process Diagram
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PCR Machine
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Links: http://
www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm
http://www.youtube.com/watch?v=eEcy9k_KsDI