Patronin Regulates Micro Tubule Network
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Patronin Regulates thePatronin Regulates theMicrotubule Network byMicrotubule Network by
Protecting Microtubule Minus Protecting Microtubule Minus EndsEnds
Sarah S. Goodwin and Ronald D. ValeCell 143, 263–274, October 15, 2010
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Tubulin assembles into polar filaments with dynamic plus and minus ends.
However, in living cells, most microtubule minus ends are static.
Goodwin and Vale identify Patronin as a protein that stabilizes minus ends and protects them from depolymerization in vivo.
They further show that purified Patronin binds selectively to minus ends and shields them from a microtubule depolymerase, Kinesin-13.
Patronin contributes to • proper organization of the microtubule cytoskeleton and • formation of the mitotic spindle,
indicating that these structures are regulated by competing actions of destabilizing and stabilizing proteins acting on minus ends
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• Patronin protects microtubule minus ends from depolymerases in vivo
• Without Patronin, microtubule organization is defective in interphase and mitosis
• In vitro Patronin can protect minus ends from Kinesin-13-catalyzed depolymerization
• Similar to plus ends, stabilizing and destabilizing proteins regulate minus ends
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Brian P. O'Rourke David J. Sharp Microtubule Dynamics: Patronin, Protector of the Minus EndCurrent Biology, Volume 21, Issue 2, R77-R79, 25 January 2011