NAT Standards for Clinical Virology Sally Baylis, NIBSC SoGAT XX, Warsaw 12-13 June 2007.
Parvovirus B19 Genotype 2 Plasma, Sourced from the US Dr Sally A. Baylis, Division of Virology...
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Parvovirus B19 Genotype 2 Plasma, Parvovirus B19 Genotype 2 Plasma, Sourced from the US Sourced from the US Dr Sally A. Baylis, Division of Dr Sally A. Baylis, Division of Virology Virology NIBSC NIBSC
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Transcript of Parvovirus B19 Genotype 2 Plasma, Sourced from the US Dr Sally A. Baylis, Division of Virology...
Parvovirus B19 Genotype 2 Parvovirus B19 Genotype 2 Plasma, Sourced from the USPlasma, Sourced from the US
Dr Sally A. Baylis, Division of VirologyDr Sally A. Baylis, Division of Virology
NIBSCNIBSC
Identification of Parvovirus Identification of Parvovirus Genotype 2 PlasmaGenotype 2 Plasma
Plasma obtained at NIBSC in 2000, as a diluent for Plasma obtained at NIBSC in 2000, as a diluent for validation of HCV RNA assaysvalidation of HCV RNA assays
Tested by conventional PCR using 3 sets of primers Tested by conventional PCR using 3 sets of primers of B19V (2002)of B19V (2002)
Retested in error in 2004, by a consensus real-time Retested in error in 2004, by a consensus real-time PCR for parvovirus B19 – unexpectedly high titrePCR for parvovirus B19 – unexpectedly high titre
DNA sequence analysis confirmed that presence of DNA sequence analysis confirmed that presence of gt2 B19V (Accession # EF216869)gt2 B19V (Accession # EF216869) 98.29% nt identity vs IM-81 (4904 bp)98.29% nt identity vs IM-81 (4904 bp) 97.83& nt identity vs A6 (4846 bp)97.83& nt identity vs A6 (4846 bp)
This plasma was sourced in the US; it is not a This plasma was sourced in the US; it is not a single unitsingle unit
Genotype 2 TitreGenotype 2 Titre
The plasma has a titre of 6.2 logThe plasma has a titre of 6.2 log1010 IU/ml IU/ml Agreement between consensus in-house assay & Artus Agreement between consensus in-house assay & Artus RealArtRealArtTMTM Parvo B19 LC kit) Parvo B19 LC kit)
No amplification plot in Roche assayNo amplification plot in Roche assay
330 mls of the plasma are available330 mls of the plasma are available
Plasma pools associated with this sample has a Plasma pools associated with this sample has a B19V loads between 7.6-8.5 logB19V loads between 7.6-8.5 log1010 IU/ml IU/ml
Products associated with the pools contain a Products associated with the pools contain a mixture of gt1 & gt2 B19V mixture of gt1 & gt2 B19V
No B19V infectivity can be found in the gt2 No B19V infectivity can be found in the gt2 plasma plasma
