Partition ChromatographyPartition Chromatography It is also known as Liquid-Liquid It is also known as Liquid-Liquid

Chromatography (LLC). If the mobile phrase Chromatography (LLC). If the mobile phrase is gas then it is called Gas-liquid is gas then it is called Gas-liquid Chromatography (GLC).Chromatography (GLC).

In LLC the two phase are liquids. The two In LLC the two phase are liquids. The two

liquids must be immiscible. The stationary liquids must be immiscible. The stationary liquid is present as thin film on an inert solid liquid is present as thin film on an inert solid support and it is usually the more polar liquid support and it is usually the more polar liquid (aqueous phase). Separation accurse due to (aqueous phase). Separation accurse due to the difference in partition coefficients of the difference in partition coefficients of solutes between the two liquids. solutes between the two liquids.

Examples of supports:Examples of supports:

1- Filter paper. 2- Cellulose 1- Filter paper. 2- Cellulose powder.powder.

3- Kieselguhr . 4-Silica gel.3- Kieselguhr . 4-Silica gel.

These supports the aqueous These supports the aqueous stationary phase by hydrogen stationary phase by hydrogen bonding.bonding.

Reversed phase Chromatography:Reversed phase Chromatography: The support is modified to hold the non polar The support is modified to hold the non polar

stationary phase and the mobile phase is stationary phase and the mobile phase is the more polar liquid in this case.the more polar liquid in this case.

Reversed phase silica gel is an example of Reversed phase silica gel is an example of the stationary phases in this type such as the stationary phases in this type such as RP18 (C18) / RP 8 (C8).RP18 (C18) / RP 8 (C8).

Partition Chromatography is better for Partition Chromatography is better for the separation of more polar solutes the separation of more polar solutes that may not easily eluted from the that may not easily eluted from the systems based adsorption.systems based adsorption.