PALLID STURGEON PROPAGATION 2019 Garrison Dam NFH

PALLID STURGEON PROPAGATION 2019

Garrison Dam NFH

Rob Holm

US Fish and Wildlife ServiceGarrison Dam NFH, Riverdale ND

Background/Introduction

The Pallid Sturgeon Recovery Plan (1993) established guidance for collection of wild brood fish,propagation, research needs, and reintroduction of progeny to accomplish recovery goals. Thishatchery’s role in the recovery effort focuses on the development of techniques for spawning andrearing of pallids and propagation for augmentation. Pallid Sturgeon propagation at GarrisonDam NFH began in 1997. Successful spawning has occurred annually since 1998. Stockingsfrom Garrison Dam NFH have occurred annually since 2002 in the Missouri and YellowstoneRivers from Missouri to Montana. As of the spring larval drift stocking in 2019, a total of285,379 fish and 2,473,416 fry had been directly stocked from Garrison Dam NFH to supplementrecovery. Additional eggs, fry and fish from Garrison Dam NFH have been shipped to otherparticipating recovery hatcheries and research facilities for further growth prior to stocking. Cryopreservation of milt also is a priority recovery action with Garrison Dam NFH being one ofthree hatcheries maintaining the pallid sturgeon milt repository. As of the fall 2019, 100 uniquewild males have been cryopreserved for the Upper Basin Recovery. Four have no progenystocked in any RPA and an additional 16 are not represented in all three RPAs.

Objectives

Garrison Dam NFH will be the spawning site for all wild brood collected that have notcontributed progeny into the three upper RPA’s. Pairing for family lots will be based on theresults from the Lamar FTC for broodstock selection. Milt will be shipped overnight to becryopreserved at Warm Springs FTC. We will use a 1X3 mating strategy making use of wildcollected brood fish that have not contributed progeny to the recovery effort. The cryo repositorywill serve as a backup in the event we are short ‘new’ males.

The stocking request for 2019 includes fry for a larval drift study as well as propagationaugmentation to increase the effective population size. For the larval drift study 550,000 day oldand 150,000 five day old larvae are requested. HRPS yearling stocking numbers will dependupon the number of adults captured that have not contributed progeny. Our stocking target willapproximate the mean number of offspring released for the male families in each RPA toincrease the effective population.

RPA1 - 270 spring yearlings/familyRPA2 - 400 spring yearlings/familyRPA3 - 30 spring yearlings/family up to a maximum of 180 fish

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Approximately five thousand eggs per family (100 mls) will be sent to Gavins Point NFH forbroodstock development provided the eggs represent new family lots. Gavins Point NFH will bespawning wild (RPA 4) and captive broodstock again this year. Progeny from the captive broodprogram will be used for research.

Further studies will be conducted this year to increase larval survival. Since Bio-Oregon’sBiodiet larval feed was discontinued in 2007, our survival success has been poor. While we aretypically able to meet the stocking targets, there is concern that we are selecting for individualfish that will accept and/or assimilate the Otohime diet that is used today. Many studies of larvalfish have shown that the digestive tracts of larval fishes lack enzymes needed to break down themore complex proteins found in processed fish feeds. This year we will be experimenting with adiet, KilliFeast, produced by Aquanix whose primary ingredients are marine fish hydrolysate,followed by zooplankton and mysis. These ingredients prepared by low-temperature dryingsuggest that there will be better retention of vitamins, lipids and amino acids. The hydrolysis offish byproducts provides more readily available simple proteins for the larval fish to digest whichshould improve survival.

The following two charts help illustrate the survival issues we have see. In 2004 we werefeeding Biodiet for the first few months, in 2017 Otohime. Although both charts show anincrease in mortality around the typical day 18 starvation period, the overall survival rates aremuch lower in 2017 than in 2004. Also note the similarities in mortality spikes based on femalelot and that not all mortality spikes are associated with starvation.

Chart 1. Larval Mortality by Family Lot in 2004 Feeding Biodiet Diets.

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Chart 2. Larval Mortality by Family Lot in 2017 Feeding Otohime Diet.

Spring Capture 2019

April 17 - Female code 124 (4A49070E13) was located by radio 5 miles upstream of the HWY85 bridge near Williston captured and taken to the hatchery. Black eggs were biopsied at captureand the PI reading was 0.2.

April 22- Female code 39 (7F7D7C2E4B) was captured at the confluence - first day of targetedbrood capture. This fish was taken to the hatchery. Both 0E13 and 2E4B were spawned atGarrison Dam NFH in 2017 and will be used in the larval drift study. Seven boats fishing thisweek.

April 24 - Two females captured, code 127 (4443250A24) and code 87 (1F48421542). Water inthe river warmed to 55F today. Tank temperatures were increased slightly to 53F.

April 29 - Water temp in river back down to 47F as a result of the snow storm that passes overthe weekend. Six boats fishing. Four at the confluence, one in the Yellowstone and one

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upstream on the Missouri. No fish captured.

April 30 - An additional boat from USGS and a representative from Lotech onsite to evaluate theradio transmitters. Air temperature overnight in the low 20's with day highs in the low 40's. Three fish captured today and taken to the hatchery, one female (4315327C7B) and two males,code 23 (1F4A31445) and code 179 (4718447879). May 21 - Three boats fishing half day - no captures

May 22- Three boats fishing in the Missouri River with two fish collected, female, code43(220F01755C) and male code 15(220F0F7677). Temps in the river had dropped to 50F andflows are coming up.

No additional broodstock were collected.

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Table 1. RPA 2 Pallid Broodstock Captures

Year PallidsCollected

FemalesCollected

MalesCollected

Unknowns

1988 3 1 1 11989 5 1 0 41990 5 1 1 31991 11 1 4 61992 38 4 16 181993 27 4 5 181994 66 6 23 371995 22 5 12 51996 20 5 12 31997 24 6 14 41998 14 3 9 21999 17 5 9 32000 26 4 17 52001 22 5 16 12002 30 8 21 12003 53 7 39 72004 65 16 48 12005 44 11 28 52006 66 14 49 32007 44 12 32 02008 62 15 47 02009 51 8 43 02010 40 5 35 02011 10 3 6 12012 43 9 34 02013 31 7 23 1

2014 25 5 20 02015 27 3 24 02016 17 8 25 12017 37 8 28 12018 3 0 3 02019 9 6 3 0Total 963 196 647 131% of individuals

captured thisyear

9% 3% 0%

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Table 2. Adults Captured in 2019

2019 Pallid Broodstock Captures

PIT Tag PIT Tag 2 Code CaptureDate

Weightlbs

SexLast

SpawnYear

CryoYear

Stocked ProgenyComments

RPA1 RPA2 RPA3

4A49070E13 124 4/17/19 F 603 696 46 Spawned in 2017 at GAD 7F7D7C2E4B 39 4/22/19 37.4 F 611 441 22 Spawned in 2017 at GAD

4443250A24 127 4/24/19 59.4 F 1176 1532 138 Spawned in 2006 at MC 1F48421542 87 4/24/19 48.4 F 2722 1488 180 Spawned in 1997, 2006, 2015 at GAD, radioed in 2015 4315327C7B 66 4/30/19 33 F 2009 827 1170 70 Spawned in 2009 at GAD, radioed in 2008 4718447879 179 4/30/19 61.6 M 2009 2009 283 436 24 Spawned in 2009 at GAD, new radio in 2013

1F4A3E1445 1F4A2F3A2E 23 4/30/19 35.2 M 2007 2007 970 2696 394 Spawned in 2002 & 2004 at GAD, and at MC 2006 & 2007

220F01755C 2204657963 43 5/22/19 52.3 F 2009 12099 20408 264 Spawned in 1999 at GAD, and at MC 2009, radio implanted in 2017

220F0F7677 15 5/22/19 35.2 M 2004 2004 13 1277 0 21,288 fry released in RPA2, Spawned at GAD in 2004

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Spawning and Propagation

June 3 - Oocytes were taken from all females collected. Female 220F0177C had immature eggs.

The other four showed no response to the progesterone assay and PI ranged from 0.09 to 0.16.

Female 4443250A24, with a PI of 0.09 was moved to the south tank to slow her PI progression

while those in the north tank had the water temperature increased to speed up the oocyte

migration.

June 17 - Oocytes taken from female 2E4B and evaluated with the progesterone assay. No

response to the progesterone. The PI was at 0.115 with very low standard deviation. The rate of

migration was determined to be 0.0032/day putting her at 0.088 on June 25th.

June 23 - Hormone injections were administered to initiate spawning at 10:00 pm

June 24 - Resolving dose administered at 10:30 am. Neurulated eggs from Gavins Point captive

brood spawn arrived at 5:30 pm. Thirteen family lots received for the larval drift study.

June 25 - Ovulation noted at the midnight check with female 4443250A24 releasing eggs. She

was spawned approximately every 30 minutes until 4:30 when a second female, 4A49070E13

initiated ovulation. See spawning results on Table 5 through Table 10. Eggs from Gavins Point

NFH are hatching beginning at 2:30 am and progressing throughout the day.

June 27 - Eggs from the 6/20 captive spawn at Gavins Point NFH completed hatch in the morning

hours. Half of the family lots are behaving a little unusual with the larvae hanging low and

toward the center of the tank rather than the typical random dispersal throughout the tank. Female

broodstock are fully stripped to expel all remaining ovulated eggs.

June 28 - Significant losses of larvae from the Gavins Point NFH lots - what we have termed the

‘early life stage mortality syndrome’ and have seen periodically throughout pallid propagation

(see mortality records, Table 15 and Chart 4). Oxygen levels were checked and at 7.5 -8 ppm.

Nitrogen readings were up to 105% with a delta P of a minus 10. A storm was occurring

overnight causing a change to the barometric pressure accounting for the rise in nitrogen. Eggs

from the second captive spawn (June 25) at Gavins Point NFH transferred by FWS FWCO offices

today and put in incubation jars.

June 29- Losses continue from the 6/20 spawn. Temperature is increased a couple degrees to

speed up hatch at 9:00 pm.

June 30 - Hatch of the 6/25 spawns occurring at ~10:00 am. Temperature again increased to 68<F

to advance hatchoff by morning.

July 1 - Preparations for packaging begins at 4:00 am with sample counts taken using the

Xpertcount larval counter. Additional staff arrive at 5:00 to begin bagging larvae. Water

temperature was turned down to 60< F for the trip and to match up to release temperatures. By

6:00 am ten staff are assisting with netting, enumerating, and bagging larvae at 200 mls per bag.

500 ml graduated cylinders were used to measure out larvae in 100 ml increments to avoid having

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larvae in the cylinder for an extended time. The trailer with larvae pulled out of the hatchery at

8:45 am headed for the Culbertson, MT release site. See Table 12 & Table 13 for larval release

information.

July 2 - Received fish health report from Bozeman FHC on the wild broodstock. Male 1445 was

positive for MRSIV via standard and qPCR. The others were clear. Male 1445 was also the only

male that produced milt this year and so is represented in all family lots produced at Garrison

Dam NFH this year.

July 3 - Captive spawn from Gavins Point NFH (6/20/2019) 30% settled to bottom

July 5 - Captive spawn from Gavins Point NFH (6/20/2019) 70% settled to bottom

July 6 - Larvae from 6/20 spawn losing plugs - initiated feed with 10 mls Otohime B2 diet/tank

July 8 - Larvae from June 25th spawn 50% settled to bottom. Moved captive brood larvae from

FT tanks to production tanks and redistributed wild spawn larvae to the 30 FT tanks for the feed

study at 300 larvae per tank. Surplus fish inventoried and moved to tank D7.

July 22 - Mortality has spiked in several family lots from the captive brood adults. The mortality

counts are based on volume samples rather than counts at this time.

July 31 - Fish from each of the captive progeny are sampled for initial month’s growth. Fish are

averaging 1.6 inches in length and 0.26 grams/fish. Feed was primarily Otohime B2 feeding out

2423 grams over the month and an additional 143 grams C1 the last two days of July. The Feed

Study is showing similar results for the Portman and Otohime diets - with 15% and 16% survival

to feed respectively. The Razerback diet has 24% survival to date. The results don’t account for

growth which appear to favor the Otohime and Portman’s diets. Although not part of the Feed

Study, an alternate feed we are providing a single tank appears to be much superior to all the

others - Golden Pearl Reef and Larval Fish diet 200-300 micron size. The feed is available

through www.brineshrimpdirect.com with an ingredients list of marine fish, krill (23%) and fish

roe as the top three. 55% protein, 15% Lipids, 12% ash and 8% moisture (see results pp. 98-99).

August 2 - Female 7F7D7C2E4B found dead in the tank. Necropsy didn’t provide any clues as to

a cause of death. No internal or external hemorrhaging except on the gill tissue.

August 3 - Two additional brood fish found dead in the brood tanks - on in the south (male

1F4A3E1445) and one in the north 20 foot tank (female 4443250A24 ). The forage fish (rainbow

trout) were also dead. The remaining six brood fish were returned to the Missouri River

Confluence.

Sept - Noted that several siblings from the family 7119 X 257F were ‘proportionally short’ as

compared to their siblings or other in the cohort.

Sept 9 - Call from Recovery Team decision not to stock any progeny from the captive broodstock.

The concerns for stocking the progeny were: 1) the potential over-representation from lower

priority males, 2) confounding results from drift study, 3) if there were higher survival of larval

from the drift study the additional stockings could exacerbate the effect (see Appendix 17. p100).

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http://www.brineshrimpdirect.com.

Table 3. Broodstock Data

2019 PALLID STURGEON BROODSTOCK DATA

Tag Number Date

Collected

Sex Wt

(Kg)

5 mgLH-RH/10 ml

saline

Last

PI

Tank 2019 Spawning Result Previous Spawn Results

(mls) (mg ) Dose

(mg/kg)

0.05 or

0.02

CryYear

Progeny (RPA)

1 2 3

4A49070E13 4/17/19 F 15 1.50 0.75 0.05 0.13 N Larvae released 603 696 467F7D7C2E4B 4/22/19 F 17 1.70 0.85 0.05 0.115 N Larvae released, died PS 611 441 224443250A24 4/24/19 F 27 2.70 1.35 0.05 0.091 S Larvae released, died PS 1176 1532 1381F48421542 4/24/19 F 22 2.20 1.10 0.05 0.125 N Larvae released 2722 1488 1804315327C7B 4/30/19 F 15 1.50 0.75 0.05 0.135 N Larvae released 827 1170 704718447879 4/30/19 M 28 1.12 0.56 0.02 N Did not spermiate 2009 283 436 241F4A3E1445 4/30/19 M 16 0.64 0.32 0.02 N Larvae released, died PS 2007 970 2696 394220F0F7677 5/22/19 M 16 0.64 0.32 0.02 Did not spermiate 2004 13 1277 0

TOTAL 12.00 6.00

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Table 4. Milt Collections

Pit Tag # Inj

Date

Inject

Time

Take

Date

Time Amount

(mls)

Motility

(fresh)

Characteristics Last

cryo

year

Milt

Cryo

2019

Motility

(at freeze)

5 ml

straws

0.5 ml

straws

1F4A3E1445 6/23 10:00p 6/25 XX 2007220F0F7677 no milt4718447879 no milt

100 wild males now represented in the repository. No straws were frozen in 2019.

Table 5. Stocking Targets by RPA to maximize Ne

Approximate RPA1 RPA2 RPA3Female Median YE 560 734 102Male Median YE 352 661 63

Overall Median YE 695 1277 70

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Table 6. Proposed Stocking Targets for Progeny of Captive Broodstock

PIT Tag PIT Tag Wild Parent Cryo Current YE Stocked Recaptures (to 2019) Target stocking #

Female Male F/M/F/M Yr RPA1 RPA2 RPA3 RPA1 RPA2 RPA3 RPA1 RPA2 RPA3 TOTAL

4624276027 0A14082E10 7F7B016070 556 0 224 4 0 34

0 0 0 01F4A363031 2007 702 395 68 10 17 9

7F7B026102 1787 4454 155 48 115 15

7F7B023253 2005 1187 1719 165 55 33 18

6C00090924 423C0C464E 114476216A 25 3174 170 1 35 18

0 0 0 0220F107A6F 2016 575 2173 144 5 171 22

7F7B021573 0 478 181 0 29 17

7F7D441774 X 0 159 66 0 6 7

42570A387A 4626226048 220E345E09 0 734 140 0 39 36

270 0 0 2701F4A27214F 2001 3 378 195 0 23 42

115676635A 245 1822 436 23 54 27

7F7D2D723D 2008 1196 7611 352 49 89 26

6C00093506 471C52622E

115679394A 0 0 0 0 0 0

0 250 0 250

1F47760123 2007 1654 450 141 18 13 16

7F7B021573 0 478 181 0 29 17

7F7D441774 0 159 66 0 6 7

11


1F4A125B2A 462446145B

1F4A301354 290 103 76 134 31 41

0 300 0 3001F4A4A1439 X 295 314 156 81 11 22

132213574A 2002 1422 39 99 25 8

7F7B023253 2005 1187 1719 165 45 31 17

462546534B 4626214B69

114476216A 25 1343 170 1 35 18

0 0 0 0430E452777 2010 0 1343 20 0 15 2

4443240458 1200 4427 96 80 118 8

444334021A 2008 935 2268 63 91 70 2

4627716D2D 4628382929

114476216A 25 3174 170 1 35 18

0 0 0 0220F107A6F 2017 575 2173 144 5 171 22

115557165A 8603 12130 448 189 279 44

7F7D2D723D 2008 1196 7611 352 49 89 26

46257D6F35 423A314D00

114476216A 25 3174 170 1 35 18

0 0 0 0220F107A6F 2016 575 2173 144 5 171 22

7F7B021573 0 478 181 0 29 17

7F7F06583D 2016 151 315 129 35 24 16

4627787556 6C00090633

114476216A 25 3574 170 1 35 18

0 0 0 0220F107A6F 2016 575 2173 144 5 171 22

7F7B026102 1787 4454 155 44 115 14

7F7B023253 2005 1187 1719 165 45 31 17

12


4624380D66 424F2F3D60

115679394A 0 0 0 0 0 0

270 400 30 7001F47760123 2007 1654 450 141 18 13 16

7F7B021573 0 478 181 0 29 17

7F7F06583D 2016 151 315 129 35 24 16

6C00096611 471C52622E

7F7B016070 566 0 224 4 0 34

270 250 0 5201F4A363031 2007 702 395 68 6 16 7

7F7B021573 0 488 181 0 29 17

7F7D441774 X 0 159 66 0 6 7

4256437C5F 423A2C5022

116224546A 0 3986 601 0 236 90

270 240 0 5101F477B3A65 2002 404 1541 188 14 61 29

7F7B021573 0 478 181 0 29 17

113719262A X 0 160 65 0 11 7

444175202F 423A314D00

7F7B016070 556 0 224 4 0 34

270 0 0 2707F7D365422 2009 727 1144 159 32 40 26

7F7B021573 0 478 181 0 29 17

7F7F06583D 2016 151 315 129 45 31 17

46237D0338 4627764F65

115679394A 0 0 0 0 0 0

270 0 0 2701F47760123 2007 1654 450 141 18 13 6

114476216A 25 3174 170 1 35 18

430E452777 2010 0 1343 20 0 12 3

13


46255B170B 423F2F737D

454910202B 147 2281 41 1 12 4

270 400 30 700220F0F7677 2004 13 655 0 0 2 0

7F7F056171 0 200 98 0 15 15

1F47760123 2007 1654 450 141 18 13 16

4628391106 4625703115

132211792A 195 186 513 0 1 61

245 0 0 2451F4A312640 2004 25 489 67 0 2 8

115551683A 185 379 44 2 7 7

115552116A 2004 1702 871 65 7 13 6

46254E3E19 487F24652B

115555495A 0 0 26 0 0 4

270 400 0 670431565767B 2007 1884 527 65 18 11 6

454B380D60 789 2734 127 16 24 15

7F7D376F73 2015 473 553 80 27 15 7

44433E701A 46273A082B

132256586A 538 581 202 8 26 25

0 0 0 01F47760123 2007 1654 450 141 18 13 16

116224546A 0 3986 601 0 236 90

1F477B3A65 2002 404 1541 188 14 61 29

425708072B 4625537512

411D262C1F 2247 1781 280 11 146 59

270 215 20 505411D0B4E09 2016 588 414 10 3 33 0

115555495A 0 0 26 0 0 4

431565767B 2007 1884 527 65 18 11 6

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46242E2A6F 462663187F

7F7F054855 1841 1886 89 16 55 9

0 0 0 0132313521A 2016 327 368 76 8 27 0

116224546A 0 3986 601 0 236 90

1F477B3A65 2002 404 1541 188 14 61 29

4624485842 6C00096524

7F7B016070 566 0 224 4 0 34

0 0 0 01F4A363031 2007 702 395 68 6 16 7

116224546A 0 3986 601 0 236 90

1F4A27214F 2001 3 378 195 0 23 42

444267561D 425669693F

7F7B016070 556 0 224 4 0 34

270 400 0 6707F7D365422 2009 727 1144 159 32 40 26

116224546A 0 3986 601 0 236 90

220F107A6F 2017 575 2173 144 5 171 22

6C00090926 46256E7712

115555495A 0 0 26 0 0 4

220 215 0 435431565767B 2007 1884 527 65 18 11 6

132211792A 195 186 513 0 1 55

7F7D487531 2004 50 246 83 19 39 12

4624127119 462616257F

7F7F054855 1841 1886 89 16 65 9

270 0 0 270132313521A 2016 327 368 76 8 27 0

116224546A 0 3986 601 0 236 90

116167123A 2008 4 661 144 0 35 12

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Table 7. Female # 4443250A24 - Spawning Results

FEMALE # 4443250A24 Spawn Date - 6/25/19 Hatch - 6/30/2019

Time MALE # Milt Data Inventory

at hatch% neur Larvae

mls

released

Larval

DriftReleases

Survival from

hatch to:

Tank

Milt mls

/ H20 mls

Date Motility mls at

GDNFH

Total eggs

@ 38.2/ml

1st

week

4th

week

10:00 p initial injection

10:00 a resolving dose

12:35 a 1F4A3E1445 205 7831

32881 95% 383 26010 94% FT112:50 a 1F4A3E1445 205 7831

1:15 a 1F4A3E1445 400 15280

1:48 a 1F4A3E1445 545 20819 20819 97% 185 12546 85% FT3

2:25 a 1F4A3E1445 590 22538 24944 106% 261 17748 90% FT4

2:55 a 1F4A3E1445 490 18718 18997 98% 99 9732 79% FT5

3:45 a 1F4A3E1445 700 26740 26197 103% 243 16524 87% FT6

4:28 a 1F4A3E1445 620 23684 23302 101% 180 12240 83% FT7

5:42 a 1F4A3E1445 530 20246 18812 92% 140 9486 83% FT8

6:28 a 1F4A3E1445 310 1184220078 94% 180 12240 91% FT2

7:40 a 1F4A3E1445 220 8404

TOTAL 4815 183933 186030 101% 1671 116526

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Table 8. Female # 4A49070E13 - Spawning Results

FEMALE # 4A49070E13 Spawn Date - 6/25/19 Hatch - 6/30/2019



mls

released

Larval

DriftReleases

Survival from

hatch to:

Tank

Milt mls

/ H20 mls


GDNFH

Total eggs

@ 50/ml

1st

week

4th

week



4:35 a 1F4A3E1445 820 41000 43762 107% 455 38633 6% FT9

5:50 a 1F4A3E1445 570 28500 29665 104% 324 28188 3% FT10

6:45 a 1F4A3E1445 360 1800027293 91% 293 25155 5% FT12

7:42 a 1F4A3E1445 240 12000

8:36 a 1F4A3E1445 125 625019258 135% 213 18344 2% FT21

9:12 a 1F4A3E1445 160 8000

TOTAL 2275 113750 119978 105% 1285 110320

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Table 9. Female # 4315327C7B - Spawning Results

FEMALE # 4315327C7B Spawn Date - 6/25/19 Hatch - 6/30/2019



mls

released

Larval

DriftReleases

Survival from

hatch to:Tank

Milt mls

/ H20 mls


GDNFH

Total eggs

@ 43/ml

1st

week

4th

week



6:00 a 1F4A3E1445 280 1204024592 79% 137 10944 92% FT16

7:10 a 1F4A3E1445 440 18920

9:00 a 1F4A3E1445 340 1462035194 117% 311 28877 91% FT22

9:55 a 1F4A3E1445 350 15050

8:22 a 1F4A3E1445 500 21500

22835 71% 159 12744 91% FT1510:25 a 1F4A3E1445 100 4300

11:00 a 1F4A3E1445 150 6450

TOTAL 2160 92880 82621 89% 607 52565

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Table 10. Female # 1F48421542 - Spawning Results

FEMALE # 1F48421542 Spawn Date - 6/25/19 Hatch - 6/30/2019



mls

released

Larval

DriftReleases

Survival from

hatch to:

Tank

Milt mls

/ H20 mls


GDNFH

Total eggs

@ 44/ml

1st

week

4th

week



6:52 a 1F4A3E1445 470 20657 20472 99% 185 15129 92% FT13

7:50 a 1F4A3E1445 670 29447 29099 100% 254 20304 96% FT14

8:25 a 1F4A3E1445 290 1274631442 86% 367 29376 96% FT19

9:48 a 1F4A3E1445 540 23733

9:05 a 1F4A3E1445 420 1845931970 117% 383 30600 96% FT23

10:30 a 1F4A3E1445 200 8790

6:12 a 1F4A3E1445 450 1977839992 102% 468 36504 97% FT11

10:58 a 1F4A3E1445 440 19338

TOTAL 3480 153120 152975 100% 1657 131913

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Table 11. Female # 7F7D7C2E4B - Spawning Results

FEMALE # 7F7D7C2E4B Spawn Date - 6/25/19 Hatch - 6/30/2019



mls

released

Larval

DriftReleases

Survival from

hatch to:

Tank

Milt mls

/ H20 mls


GDNFH

Total eggs

@ 44.3/ml

1st

week

4th

week



6:10 a 1F4A3E1445 70 3100

4366 16% 30 2400 94% FT176:55 a 1F4A3E1445 200 8856

9:52 a 1F4A3E1445 340 15055

10:27 a 1F4A3E1445 230 10184

5753 14% 50 4000 90% FT1810:59 a 1F4A3E1445 350 15498

7:45 a 1F4A3E1445 320 14170

8:30 a 1F4A3E1445 290 1284119505 77% 104 8320 99% FT20

9:10 a 1F4A3E1445 280 12398

TOTAL 2080 92144 29624 32% 184 14720

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Table 12. Gavins Point NFH Captive Broodstock Larval Releases and Survival

Gavins Point NFH Captive Broodstock Larval Releases and Survival

Female # Male # Hatched

Larvae

*Larval

Releases

Age of

Larvae

dph

Inventory

post LD

releases

Tank

#

Inventory

at 31 dph1 month %

Survival

4624276027 0A14082E10 27,576 7,560 5 392 C1 43 0.2%

6C00090924 423C0C464E 14,328 0 - 0 - 0 0.0%

42570A387A 4626226048 48,364 39,744 5 3,085 C3 664 8.8%

6C00093506 471C52622E 23,743 2,228 5 741 C4 163 0.8%

1F4A125B2A 462446145B 34,543 10,517 5 2,361 C5 165 0.7%

462546534B 4626214B69 26,109 19,008 5 2,630 C6 219 3.2%

4627716D2D 4628382929 30,366 15,246 5 1,514 C7 236 1.6%

46257D6F35 423A314D00 44,825 33,840 5 469 C9 80 0.7%

4627787556 6C00090633 49,816 4,064 5 1,709 C10 117 0.3%

4624380D66 424F2F3D60 46,358 23,220 5 4,238 A6 1817 8.1%

6C00096611 471C52622E 31,383 12,960 5 3,332 A7 259 1.5%

4256437C5F 423A2C5022 33,715 0 5 627 A8 256 0.8%

444175202F 423A314D05 50,687 32,400 5 844 B7 215 1.2%

46237D0338 4627764F65 16,444 14,112 1 2,332 C8 202 8.7%

46255B170B 423F2F737D 35,932 27,360 1 8,554 B4 201 2.3%

4628391106 4625703115 23,217 21,735 1 1,457 C2 185 12.5%

46254E3E19 487F24652B 32,203 27,306 1 4,897 B6 438 8.9%

44433E701A 46273A082B 36,580 33,840 1 2,740 B8 212 7.7%

425708072B 4625537512 38,934 33,579 1 5,355 A1 653 12.2%

46242E2A6F 462663187F 43,995 40,014 1 3,981 A2 779 19.6%

4624485842 6C00096524 40,941 39,312 1 1,629 A3 10 0.6%

444267561D 425669693F 48,897 43,326 1 5,571 A4 1226 22.0%

6C00090926 46256E7712 50,206 48,222 1 1,984 A5 307 15.5%

4624127119 462616257F 27,039 9,234 1 17,805 B5 1797 10.1%

Total 856,201 538,827 78,247 10,244 3.5%

* larval release numbers are estimates and are adjusted down 10% to offset water weight from netting larvae

5 dph larvae = 200,787 1 dph larvae = 338,040

21

Table 13. Egg Hatch and Fry Survival

Egg Hatch and Fry Survival Summary - Garrison Dam NFH

FEMALE # MALE # Eggvolume

(mls)

Egg size(#/ml)

Number % Hatch Inventoryend of first

week(7/7/2019)

Mortality toweek 1

% frySurvival for

week 1

LarvalDrift

Releasemls

#larvae/

ml

Larval DriftRelease

4443250A24 1F4A3E1445 4815 38.2 183,933 114% 72,504 23,677 67% 1670 68 113,526

4A49070E13 1F4A3E1445 2275 50.0 113,750 111% 9,963 5,430 45% 1284 86 110,319

4315327C7B 1F4A3E1445 2160 43.0 92,880 97% 30,071 7,202 76% 607 87 52,565

1F48421542 1F4A3E1445 3480 44.0 153,120 104% 22,336 5,325 76% 1656 80 131,913

7F7D7C2E4B 1F4A3E1445 2080 44.3 92,144 33% 7,736 1,023 87% 274 80 21,888

TOTAL 14,810 635,827 97% 142,610 42,657 70% 5491 78 430,211

22

Table 14. Survival Summary

& %July 1

InventoryProposed

StockTarget

Cull Tank Monthly Mortality Sept 18Inventory

%SurvJuly-SeptJuly Aug Sept

4624276027 0A14082E10 392 43 C1 349 2 1 40 10%

6C00090924 423C0C464E 63 0 14 C2 49 - - 0 0%

42570A387A 4626226048 3085 270 C3, B1 2421 42 0 622 20%

6C00093506 471C52622E 741 167 C4 578 4 0 159 21%

1F4A125B2A 462446145B 2361 166 C5 2196 6 1 158 7%

462546534B 4626214B69 2630 0 C6 2411 19 0 200 8%

4627716D2D 4628382929 1514 0 C7 1278 30 0 206 14%

46257D6F35 423A314D00 469 0 C9 389 3 1 76 16%

4627787556 6C00090633 1709 0 C10 1592 2 0 115 7%

4624380D66 424F2F3D60 4238 0 695 A6, B2 2421 107 0 1015 24%

6C00096611 471C52622E 3332 200 A7 3073 5 0 254 8%

4256437C5F 423A2C5022 627 257 A8 371 11 0 245 39%

444175202F 423A314D05 844 200 B7 629 1 0 214 25%

46237D0338 4627764F65 2332 306 C8 2130 34 0 168 7%

46255B170B 423F2F737D 8572 241 B4 8371 18 0 183 2%

4628391106 4625703115 1482 200 C2 1297 25 0 160 11%

46254E3E19 487F24652B 4897 0 B6 4459 43 0 395 8%

44433E701A 46273A082B 2740 45 B8 2528 40 0 172 6%

425708072B 4625537512 5355 270 A1 4702 163 0 490 9%

46242E2A6F 462663187F 3981 45 A2 3202 20 0 759 19%

4624485842 6C00096524 1629 0 10 A3 1619 - - 0 0%

444267561D 425669693F 5571 200 A4, A3 4345 89 1 1136 20%

6C00090926 46256E7712 1984 312 A5 1677 3 0 304 15%

4624127119 462616257F 17805 270 574 B5, B3 16008 223 5 995 6%

Totals/Averages 78353 3192 1293 68095 890 9 8066 10%

23

Table 15. Mortality Records to 31 Days Post Hatch

DPH /Tank A1 A2 A3 A4 A5 A6 A7 A8 B4 B5 B6 B7 B8 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10

Female 072B 2A6F 5842 561D 0926 0D66 6611 7C5F 170B 7119 3E19 202F 701A 6027 1106 387A 3506 5B2A 534B 6D2D 0338 6F35 7556Male 7512 187F 6524 693F 7712 3D60 622E 5022 737D 257F 652B 4D05 082B 2E10 3115 6048 622E 145B 4B69 2929 4F65 4D00 0633

1 328 702 128 135 401 473 384 39 1698 931 324 383 1386 446 2433 2310 348 1001 84 608 430 2402 383 223 711 535 211 102 27 20 98 550 343 44 267 2734 77 426 355 4490 1388 1864 304 4938 43453 608 589 510 612 432 125 22 8 288 686 775 18 454 7714 36 464 11020 2784 580 7540 461 1985 18674 918 663 157 1071 255 43 13 16 305 2244 576 21 234 7524 44 1238 6336 11385 1237 3100 125 2100 33250

5 93 136 20 220 11 18 15 10 56 381 84 1 217 266 15 974 753 2658 265 1018 72 1063 43416 101 70 26 141 24 9 25 5 81 553 73 29 60 89 8 864 270 1845 1936 792 60 235 12047 34 96 7 70 21 42 93 3 73 310 21 5 44 5 5 91 41 136 26 38 25 32 698 69 62 6 66 5 31 39 8 49 199 9 17 36 16 7 48 24 36 31 10 32 11 669 18 16 3 41 7 4 5 6 13 54 0 3 28 24 7 36 19 24 12 8 18 4 4910 11 21 2 28 7 8 12 5 29 7 2 3 36 7 5 3 3 7 1 2 6 3 2211 25 62 4 93 12 41 19 32 45 54 6 19 40 0 44 4 5 5 0 3 1 4 012 3 32 0 85 26 49 17 20 46 563 38 24 44 3 13 9 3 1 1 11 11 0 113 42 22 11 30 25 56 39 11 32 451 8 21 5 6 8 3 1 5 3 1 13 3 2114 12 21 7 27 11 32 56 14 24 212 3 14 7 1 6 3 8 1 1 1 9 0 515 15 76 8 11 62 28 133 9 25 77 0 12 20 5 13 22 1 4 2 0 3 2 316 13 64 9 57 34 26 373 3 27 269 8 8 99 8 1 18 7 14 42 15 10 1 2817 16 38 3 96 23 101 658 17 74 268 14 8 60 3 19 22 14 12 86 29 29 5 2118 15 53 2 142 21 372 675 9 129 270 26 1 64 1 34 82 24 28 51 13 48 15 1719 18 40 1 152 20 387 381 5 331 407 28 4 47 8 38 83 13 11 59 4 88 5 1020 49 11 1 85 12 205 56 17 286 261 36 14 48 1 17 32 11 9 44 8 84 7 221 102 13 0 36 10 138 10 30 351 92 136 4 45 7 74 34 6 6 24 6 40 12 422 258 18 0 51 6 40 1 31 1343 406 486 13 78 9 135 39 24 12 26 24 21 5 323 276 22 3 36 8 15 3 19 1251 2845 553 3 166 15 97 231 48 9 9 51 17 1 924 481 13 0 36 2 9 3 14 426 3212 209 4 14 25 35 98 15 12 10 118 13 19 725 423 31 0 54 3 0 5 7 320 1085 53 1 2 26 9 183 13 8 11 67 8 5 626 260 29 0 190 10 6 0 4 878 193 17 8 8 43 13 248 8 2 13 24 5 5 1427 45 22 0 95 10 2 4 1 11 87 10 2 1 29 24 73 1 2 7 16 7 6 1328 34 22 0 64 3 11 2 5 8 176 8 0 3 6 21 27 1 0 1 20 0 5 029 14 18 0 79 0 16 1 2 34 58 3 2 5 6 17 26 5 0 2 5 5 2 430 15 6 0 2 4 13 1 1 23 27 0 2 6 2 15 24 3 0 5 0 2 0 331 23 11 0 5 1 19 1 0 17 11 3 0 7 3 14 21 2 0 2 0 5 0 0

Beginning # 5355 3981 1629 5571 1984 23138 18423 33715 8572 17805 4897 18287 2740 20016 1482 8620 21515 24026 7101 15120 2332 10985 45752

Mortality 4702 3202 1619 4345 1677 21260 18155 33450 8371 16008 4459 18066 2528 19972 1297 7859 21344 23854 6876 14872 2130 10903 45624

Ending # 653 779 10 1226 307 1817 259 256 201 1797 438 215 212 43 185 664 163 165 219 236 202 80 117% mortality 88% 80% 99% 78% 85% 92% 99% 99% 98% 90% 91% 99% 92% 100% 88% 91% 99% 99% 97% 98% 91% 99% 100%

24

Chart 3. Daily Mortality - Captive Brood Progeny to 31 DPH

25

Table 16. Initial Growth and Survival

Female # Male # SampleCount #

7/31/19

AverageWt.

(g)/fish

AverageLength

(in)

TotalWeight

(g)

SampleCount #

8/26/19

AverageWt.

(g)/fish

AverageLength

(in)

TotalWeight

(g)

Growth

(inches)

GrowthRate

(in/day)

Family GrowthPerformance

(% of Average)

4624276027 0A14082E10 21 0.33 1.7 6.9 7 2.9 3.6 20 1.9 0.031 95%

42570A387A 4626226048 18 0.39 1.8 7.0 32 2.8 3.5 90 1.7 0.028 86%

6C00093506 471C52622E 23 0.26 1.6 6.0 25 1.8 3.0 45 1.4 0.023 71%

1F4A125B2A 462446145B 23 0.26 1.6 6.0 25 1.2 2.6 30 1.0 0.016 51%

462546534B 4626214B69 24 0.38 1.8 9.1 29 1.9 3.1 55 1.3 0.021 66%

4627716D2D 4628382929 26 0.15 1.3 3.9 16 1.9 3.1 30 1.8 0.030 91%

46257D6F35 423A314D00 22 0.02 1.4 0.4 20 1.0 2.5 20 1.1 0.018 56%

4627787556 6C00090633 14 0.40 1.9 5.6 26 2.2 3.3 57 1.4 0.023 71%

4624380D66 424F2F3D60 150 0.25 1.6 37.5 63 1.8 3.1 113 1.5 0.025 76%

6C00096611 471C52622E 60 0.33 1.7 19.8 35 2.8 3.5 98 1.8 0.030 91%

4256437C5F 423A2C5022 49 0.43 1.9 21.1 20 4.2 4.0 84 2.1 0.034 106%

444175202F 423A314D05 32 0.38 1.8 12.2 22 2.1 3.2 46 1.4 0.023 71%

46237D0338 4627764F65 25 0.10 1.2 2.5 29 1.7 3.0 49 1.8 0.030 91%

46255B170B 423F2F737D 24 0.17 1.4 4.1 24 2.2 3.3 53 1.9 0.031 96%

4628391106 4625703115 14 0.30 1.7 4.2 18 2.2 3.3 40 1.6 0.026 81%

46254E3E19 487F24652B 37 0.14 1.3 5.2 43 1.6 2.9 69 1.6 0.026 81%

44433E701A 46273A082B 19 0.26 1.6 4.9 19 3.5 3.8 66 2.2 0.036 111%

425708072B 4625537512 60 0.10 1.0 6.0 48 0.2 3.0 9 2.0 0.033 101%

26

Female # Male # SampleCount #

7/31/19

AverageWt.

(g)/fish

AverageLength

(in)

TotalWeight

(g)

SampleCount #

8/26/19

AverageWt.

(g)/fish

AverageLength

(in)

TotalWeight

(g)

Growth

(inches)

GrowthRate

(in/day)

Family GrowthPerformance

(% of Average)

46242E2A6F 462663187F 48 0.29 1.7 13.9 33 3.8 3.9 125 2.2 0.036 111%

444267561D 425669693F 107 0.20 1.5 21.4 32 2.6 3.4 83 1.9 0.068 210%

6C00090926 46256E7712 22 0.41 1.9 9.0 18 4.4 4.1 79 2.2 0.079 243%

4624127119 462616257F 72 0.31 1.7 22.3 46 1.7 3.0 78 1.3 0.046 143%

TOTALS / AVERAGES 890 0.27 1.6 237.0 630 2.3 3.3 1444 1.7 0.032

27

Table 17. Feed Amounts and Cost

2018 Pallid Feed AmountsJuly August September

day B2 C1 B2 C1 C2 #2 #2

1 160 160 1805

2 142 142 1980

3 162 162 2023

4 160 160 1830

5 216 604

6 5 475 1534

7 26 472 1666

8 38 534 1717

9 23 451 1486

10 52 636 1348

11 63 632 1352

12 73 755 1384

13 92 844 1444

14 105 683 1432

15 182 333 333 1450

16 200 367 367 1425

17 126 415 415 1389

18 144 435 435

19 105 462 462

20 102 588 588

21 95 583 583

22 89 1027

23 85 1387

24 124 1368

25 109 1405

26 78 1655

27 120 1423

28 117 1617

29 127 1532

30 70 70 1858

31 73 73 1708

Total g 2423 143 624 7437 3183 17048 25869

Total lbs 5.34 0.31 1.37 16.38 7.01 37.55 56.98

cost/lb 10.87 6.87 10.87 6.87 9.83 1.26 1.26

Total cost $58.01 $2.16 $14.94 $112.54 $68.92 $47.31 $71.80

28

Chart 4. Broodstock Tank Temperature - North 20 Foot Tank

29

Chart 5. Broodstock Tank Temperature - South 20 Foot Tank

30

Chart 6. Production Temperatures

31

Table 18. Proposed Spawning Strategy Using Relatedness Analysis

Proposed 2019 Family Crosses

Female PIT Male PIT RxyStocking Targets Lamar Priority Current # Stocked Progeny

RPA1 RPA2 RPA3 RPA1 RPA2 RPA3 RPA1 RPA2 RPA3

1F4A3E1445 0 0 0 5 5 5 955 1676 394

220F0F7677 352 661 63 2 2 2 13 655 0

4718447879 0 661 63 4 2 2 204 318 0

4A49070E13 1F4A3E1445 0 0 0 2 2 2 603 696 46

7F7D7C2E4B 1F4A3E1445 0 0 0 2 2 2 620 441 22

4443250A24 1F4A3E1445 0 0 0 4 4 2 1176 1288 138

1F48421542 1F4A3E1445 0 0 0 5 5 3 1365 1485 180

4315327C7B 1F4A3E1445 0 0 0 4 2 2 649 928 47

Family cross made indicated by yellow highlight

32

Table 19. Oocyte Maturation Assay

PIT number: 4A49070E13

Date 6/3/2019

Incubation temperature: 16EC

Start time: 4:30 pm

Finish time: 8:30 am

Hours incubated: 16

Progesterone Assay

Progesterone 1

GV intact:

GVBB:

% GVBD: ??

Progesterone 2

GV intact:

GVBD:

% GVBD: ??

Control 1

GV intact:

GVBD:

% GVBD: ??

Previous Polarity Index

Date PI S.Dev

4/18/2019 0.0205 0.0235

6/3/2019 0.1310 0.0195

Polarity Index

1 0.1277

2 0.1294

3 0.1111

4 0.1667

5 0.1087

6 0.1412

7 0.1294

8

9

10

11

12

13

14

15

16

17

18

19

20

21

Ave. 0.131

S. Dev. 0.0195

SEM 0.0074

33


PIT number: 7F7D7C2E4B

Date 6/17/2019


Start time: 4:30 pm


Hours incubated: 16

Progesterone Assay

Progesterone 1

GV intact: 10

GVBB: 0

% GVBD: 0%

Progesterone 2

GV intact:

GVBD:

% GVBD: ??

Control 1

GV intact: 10

GVBD: 0

% GVBD: 0%


Date PI S.Dev

4/22/19 0.196 0.0199

6/3/2019 0.159 0.0171

6/17/2019 0.115 0.0028

Polarity Index

1 0.1315

2 0.1215

3 0.1150

4 0.1165

5 0.1200

6 0.1143

7 0.120

8 0.106

9 0.110

10 0.122

11 0.106

12 0.095

13

14

15

16

17

18

19

20

21

Ave. 0.115

S. Dev. 0.0095

SEM 0.0028

34


PIT number: 1F48421542

Date 6/3/2019


Start time: 4:30 pm


Hours incubated: 16

Progesterone Assay

Progesterone 1

GV intact:

GVBB:

% GVBD: ??

Progesterone 2

GV intact:

GVBD:

% GVBD: ??

Control 1

GV intact:

GVBD:

% GVBD: ??


Date PI S.Dev

4/24/2019 0.153 0.018

6/3/2019 0.1250 0.010

Polarity Index

1 0.1294

2 0.1111

3 0.1196

4 0.1200

5 0.1379

6 0.1333

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

Ave. 0.125

S. Dev. 0.0100

SEM 0.0041

35


PIT number: 4443250A24

Date 6/3/2019


Start time: 4:30 pm


Hours incubated: 16

Progesterone Assay

Progesterone 1

GV intact:

GVBB:

% GVBD: ??

Progesterone 2

GV intact:

GVBD:

% GVBD: ??

Control 1

GV intact:

GVBD:

% GVBD: ??


Date PI S.Dev

4/24/2019 0.138 0.0096

6/3/2019 0.0910 0.0087

Polarity Index

1 0.0918

2 0.0808

3 0.1020

4 0.1000

5 0.0857

6 0.0842

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

Ave. 0.091

S. Dev. 0.0087

SEM 0.0036

36


PIT number: 4315327C7B

Date 6/3/2019


Start time: 4:30 pm


Hours incubated: 16

Progesterone Assay

Progesterone 1

GV intact:

GVBB:

% GVBD: ??

Progesterone 2

GV intact:

GVBD:

% GVBD: ??

Control 1

GV intact:

GVBD:

% GVBD: ??


Date PI S.Dev

4/30/2019 0.1950 0.0266

6/3/2019 0.1360 0.0262

Polarity Index

1 0.1111

2 0.1136

3 0.1765

4 0.1341

5 0.1667

6 0.1333

7 0.1158

8

9

10

11

12

13

14

15

16

17

18

19

20

21

Ave. 0.136

S. Dev. 0.0262

SEM 0.0099

37

Appendix 1. Milt Repository at Garrison Dam NFH

# Pit Tag 2nd Pit Tag 3rd Pit Tag 1 CryoYear

2 CryoYear

3 CryoYear

4 CryoYear

RPA1 RPA2 RPA3

1 0A180E0E7E 2016 329 399 30

2 114473737A 2004 0 0 63 115525534A 2006 3245 6816 1674 115529097A 2004 2006 2007 567 1565 205 115544332A 2002 0 0 06 115552116A 2004 5285 1242 657 115553761A 2005 2157 5196 738 115556461A 2002 2015 733 2136 1309 115626162A 2006 2008 6311 11969 522

10 115631222A 43154A1133 2001 1565 3514 9111 115633183A 2017 286 1787 5412 115669294A 2007 4564 3292 1913 115669540A 2003 2017 1135 1181 5914 115675486A 2003 1003 705 3015 115679374A 2004 0 1493 2416 115679523A 2009 1744 2643 2917 115712453A 2000 2006 2008 3237 10369 93518 116167123A 2002 2008 4 661 14419 132114552A 2003 2007 57 798 12820 132157621A 2003 2006 2007 557 2387 7421 132313521A 2003 2010 2016 469 743 13622 17509415139 2001 735 422 7023 1F47606357 1F4A1E4336 2004 125 1777 1724 1F47652015 2007 108 454 5525 1F4772396F 2002 2016 30 103 15

26 1F47760123 2003 2007 5713 908 141

27 1F477B3A65 2000 2002 568 2298 188

28 1F477B4E51 2004 2006 378 3704 1929 1F482F3F2B 2005 946 599 1930 1F4838134E 2008 0 0 031 1F48472D25 2007 2008 84 128 332 1F4849755B 2008 2153 6781 68033 1F4A0C2E5D 2008 0 0 034 1F4A111C6A 2001 0 368 7035 1F4A13453F 2017 346 421 2536 1F4A13592B 220D4E6A57 2003 1299 4289 38237 1F4A143350 2000 2010 469 1003 77

38 1F4A206A0D 2007 276 2216 1939 1F4A27214F 2001 3 378 19540 1F4A312640 2004 25 1886 6741 1F4A33194B 2000 2010 469 1043 7542 1F4A34194A 2007 101 936 2043 1F4A363031 2003 2007 730 713 6844 1F4A3E1445 1F4A2F3A2E 2002 2004 2007 2019 964 2706 39445 1F4A435004 2013 2017 656 403 6046 1F4A4B5973 2001 2004 510 1091 9547 1F4B225A1A 2012 926 444 8848 1F4B26036D 2007 300 2542 8149 1F5001721E 2007 2008 231 1846 6950 1F521B1E56 1F54696C38 116175735A 2003 2017 2018 297 440 6051 1F53312736 2015 600 493 6052 2202236E31 2000 0 0 053 220E3F2578 2010 334 400 3054 220E4E4E5D 2003 2009 2017 278 378 3155 220E5E551E 220D0E1F0B 2008 1772 2524 2556 220E5F4928 2004 228 1560 1957 220F0E6207 2004 484 428 1558 220F0F7677 2004 13 1277 059 220F107A6F 2002 2016 2017 573 2038 144

38

# Pit Tag 2nd Pit Tag 3rd Pit Tag 1 CryoYear

2 CryoYear

3 CryoYear

4 CryoYear

RPA1 RPA2 RPA3

60 2224076523 2006 2012 174 277 2461 411D0B4E09 17509312265 2001 776 411 12062 411D0E2C5F 2001 2003 2017 472 392 7063 41476A0462 2001 553 424 7064 424E36510D 2007 67 274 7965 424E3E6127 2008 0 0 066 424E5A5314 2008 0 0 067 425028583F 2008 0 0 068 4255534C5D 2008 0 0 069 430E452777 2004 2008 2010 0 4873 2070 43105C602B 2009 4024 5520 4371 431565767B 2007 6253 969 6572 435F71414F 220E5F6E26 7F7F054773 2000 2009 619 886 2773 435F7C1210 2006 387 352 074 4441774C6E 2006 229 1895 8675 444334021A 2008 893 3505 6376 44436E2975 2010 469 731 7577 44440A7B73 2007 2009 3573 886 7678 452738076E 2003 88 99 3179 452A4E1F15 7F7D461025 2003 0 653 14580 454B30016B 2007 2016 3166 443 2081 465B6F1939 2009 1441 2133 3782 465D43745B 2006 413 790 13883 466C473C59 2006 830 1614 084 4704550E5B 7F7B082208 2009 2010 2829 7048 10385 470A754E14 2009 1868 3100 1486 4714560E0E 2007 110 402 2087 4718447879 2009 283 436 2488 486838404F 4310624556 2011 2014 665 385 6089 6C00112905 2018 0 0 090 7F7B023253 2005 1610 2414 16591 7F7B024F2D 2012 2017 283 411 61

92 7F7B025248 2012 165 266 2893 7F7B031F17 2005 1002 4075 257

94 7F7B082C10 2009 545 733 2595 7F7D24754C 220E465E74 2010 469 1032 7696 7F7D23292E 34354025646 2016 326 400 3097 7F7D291A07 2003 2006 2007 5951 1057 26698 7F7D2D723D 2007 2008 3107 7611 66399 7F7D365422 41475D3C5D 2009 2175 3637 159

100 7F7D372A6B 2003 2006 3269 2359 64101 7F7D37642C 2009 4415 9263 185102 7F7D376F73 1F54714656 2004 2015 611 594 80103 7F7D3C5708 2001 0 247 67104 7F7D41431D 2007 2008 120 866 19105 7F7D434B54 4443422E34 2002 2006 311 1532 19106 7F7D437250 7F7D3C555D 435F557624 2004 2008 804 1626 64107 7F7D487531 132235554A 3435464185 2004 43 914 83108 7F7D4A7758 4310556551 2006 2048 3427 184109 7F7E42795C 2004 0 0 0110 7F7E55466D 1F557B2071 2004 2008 3638 14269 412111 7F7F065834 2002 2004 2016 453 1246 186112 7F7F066471 115667264A 2006 2008 4648 10927 595113 7F7F06697C 2009 1846 2750 62114 7F7F066A40 2204583665 2004 2006 726 5605 19

Cryopreserved sperm from future broodstock males at Gavins Point NFH - 14 males

PIT Tag # on cane

Stockings for need validating. Stocking #'s do not include fry. 100 wild males to date cryopreserved

39

Appendix 2. Cryopreserved Milt Repository - Upper Basin Wild

114 males are represented in the repository, 100 from the wild and 14 from the broodstock program. 45 males have been cryopreserved on morethan one occasion (repeats). Four wild males are not yet represented as progeny in the river and 16 are needed on one of the three RPA’s. Milt priorto 2008 is of poorer quality due to cryoprotectant used (HBSS pre-2008, AKOS from 2008 to present)and 0.5 ml straws are generally higher quality.

40

Appendix 3. RPA 2 Missouri River Stockings from Garrison Dam NFH

Pallid Sturgeon Augmentation in the Recovery Area 2 Missouri River by Family

Year

Class

Hatchery Female

Pit tag

Male

Pit tag

Number stocked per stock site (Fall/Spring/Total) SummerStock

Totalstocked

Culbertson Wolf Point Milk

2001 Garrison DamNFH

7F7F06672B 7F7D3C5708 - 0 0 - 0 0 - 0 0 0

7F7F06672B 115631222A - 0 0 - 0 0 - 0 0 0

411D262C1F 1F4A4B5973 - 85 85 - 85 85 - 0 0 170

411D262C1F 411D0E2C5F - 64 64 - 85 85 - 0 0 149

411D262C1F 41476A0462 - 85 85 - 85 85 - 0 0 170

411D262C1F 411D0B4E09 - 0 0 - 85 85 - 0 0 85

411D262C1F 17509415139 - 83 83 - 85 85 - 0 0 168

220E345E09 1F4A111C6A - 0 0 - 0 0 - 0 0 0

220E345E09 1F4A27214F - 0 0 - 0 0 - 0 0 0

2001 TOTAL - 317 317 - 425 425 - 0 0 742


114476216A 7F7D487531 75 - 75 309 - 309 - 0 0 384

114476216A 1F4A4B5973 150 - 150 150 - 150 - 0 0 300

114476216A 7F7E55466D 1213 - 1213 1150 - 1150 - 0 0 2363

114476216A 1F4A312640 681 - 681 681 - 681 - 0 0 1362

114476216A 430E452777 1700 - 1700 1750 - 1750 - 0 0 3450

114476216A 1F477B3A65 - - 0 - - 0 - 0 0 0

114476216A 431565767B - - 0 - - 0 - 0 0 0

41


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


115555495A 431565767B - - 0 - - 0 - 0 0 0

115551683A 7F7D3C555D - - 0 - - 0 - 0 0 0

7F7F066452 7F7F065834 - - 0 - - 0 - 0 0 0

454910202B 1F47606357 - - 0 - - 0 - 1200 1200 1200

454910202B 220F0F7677 - - 0 - - 0 - 1082 1082 1082

454910202B 115679374A - - 0 - - 0 - 1200 1200 1200

454B380D60 7F7D376F73 - - 0 - - 0 - - 0 0

454B380D60 7F7F065834 - - 0 - - 0 - - 0 0

1F5330401E - - 0 - - 0 - - 0 0

2004 TOTAL 3819 0 3819 4040 0 4040 0 3482 3482 11341

Garrison DamNFH

FRY (130,000)

114476216A 430E452777 18333 - 18333 - - 0 - - 0 18333

114476216A 1F4A312640 18333 - 18333 - - 0 - - 0 18333

114476216A 7F7E55466D 18334 - 18334 - - 0 - - 0 18334

454910202B 1F47606357 41506 - 41506 - - 0 - - 0 41506

454910202B 220F0F7677 21280 - 21280 - - 0 - - 0 21280

454910202B 115679374A 12214 - 12214 - - 0 - - 0 12214

2004 FRY TOTAL 130000 - 130000 - - 0 - - 0 130000

42


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


2005

Garrison DamNFH

7F7B026102 115553761A 651 174 825 650 180 830 485 135 620 2275

7F7B026102 7F7B023253 0 172 172 0 172 172 0 170 170 514

115676635A 1F50072169 59 70 129 78 70 148 48 78 126 403

115676635A 7F7D2D723D 100 70 170 181 80 261 97 62 159 590

115676635A 7F7B031F17 171 70 241 228 70 298 109 72 181 720

115557165A 1F50072169 273 33 306 148 70 218 110 90 200 724

115557165A 7F7D2D723D 315 70 385 246 70 316 60 80 140 841

115557165A 7F7B031F17 250 70 320 300 70 370 150 75 225 915

4443240458 115633183A 275 60 335 275 57 332 130 62 192 859

4443240458 444334021A 293 55 348 293 25 318 141 183 324 990

RUNT MIX - - - - - - - - - 0

2005 TOTAL 2387 844 3231 2399 864 3263 1330 1007 2337 8831


4443250A24 465D43745B 92 60 152 92 62 154 92 59 151 457

4443250A24 115529097A 16 55 71 16 55 71 16 46 62 204

4443250A24 435F7C1210 0 60 60 0 60 60 0 64 64 184

1F497F1801 1F4A3E1445 336 134 470 345 118 463 345 83 428 1361

1F497F1801 466C473C59 199 96 295 199 112 311 199 152 351 957

1F497F1801 444171072D 197 110 307 197 110 307 197 101 298 912

1F497F1801 1F4A0B1A72 268 116 384 268 121 389 268 121 389 1162

43


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


GAP GAP 0 13 13 0 13 13 0 13 13 39

2006 TOTAL 1108 644 1752 1117 651 1768 1117 639 1756 5276

2007 Garrison Dam 1F4A36E66 115529097A 174 11 185 174 11 185 174 23 197 14 580

1F4A36E66 115631222A 24 15 39 24 15 39 4 16 20 17 116

1F4A36E66 115552116A 13 21 34 13 21 34 13 21 34 25 128

115557165A 1F47760123 90 0 90 90 0 90 90 0 90 0 270

115557165A 431565767B 59 12 71 59 12 71 59 13 72 14 229

115557165A 7F7D291A07 89 5 94 89 5 94 89 10 99 7 294

115553544A 1F47652015 36 18 54 36 18 54 36 18 54 21 184

115553544A 115556461A 154 4 158 154 0 154 154 2 156 4 473

115553544A 4441774C6E 127 9 136 127 9 136 127 9 136 11 419

454B490528 1F48472D25 2 0 2 2 0 2 2 0 2 0 5

454B490528 424E36510D 55 8 63 55 8 63 55 8 63 10 198

454B490528 4714560E0E 50 20 70 50 20 70 50 20 70 22 232

470378405D 115556461A 150 0 150 150 0 150 0 0 0 0 301

470378405D 1F4A34194A 152 5 157 152 5 157 152 5 157 7 478

470378405D 1F4B26036D 354 26 380 354 24 378 332 29 361 26 1145

7F7B025D51 4441774C6E 146 9 155 146 9 155 146 9 155 11 477

7F7B025D51 1F5001721E 256 21 277 256 21 277 256 21 277 22 853

44


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


7F7B025D51 7F7D41431D 147 9 156 147 9 156 83 12 95 11 418

47151A3D3A 1F4A363031 81 37 118 81 27 108 81 34 115 33 375

47151A3D3A 7F7D372A6B 344 19 363 344 19 363 344 19 363 20 1109

47151A3D3A 115669294A 551 31 582 551 25 576 378 30 408 30 1596

7F7FD66963 115525534A 221 36 257 221 36 257 376 36 412 36 961

7F7FD66963 1F477B4E51 457 31 488 457 31 488 457 31 488 30 1494

7F7FD66963 220E5F4928 268 19 287 268 19 287 268 19 287 20 882

40636B2945 4443422E34 168 20 188 168 20 188 168 20 188 21 586

40636B2945 44440A7B73 44 0 44 44 0 44 45 0 45 0 133

40636B2945 115712453A 374 32 406 374 27 401 374 5 379 21 1206

454B380D60 2204583665 476 31 507 476 31 507 404 13 417 30 1461

454B380D60 132114552A 24 9 33 24 9 33 24 11 35 11 112

454B380D60 7F7D4A7758 41 0 41 41 0 41 41 0 41 0 123

47037F460C 1F4A5A5A63 321 40 361 321 32 353 321 32 353 36 1104

47037F460C 454B30016B 43 0 43 43 0 43 43 0 43 0 129

47037F460C 7F7F066471 84 23 107 84 20 104 84 25 109 21 342

4443240458 1F4A206A0D 294 25 319 294 25 319 294 35 329 27 995

4443240458 132157621A 306 32 338 306 32 338 306 34 340 32 1048

4443240458 466C473C59 0 0 0 0 0 0 0 0 0 0 0

45


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


115676635A 1F4A3E1445 31 0 31 31 0 31 13 0 13 0 74

7F7FD66963 115552116A 0 0 0 0 0 0 0 0 0 0 0

7F7FD66963 115529097A 0 0 0 0 0 0 0 0 0 0 0

40636B2945 1F4A4B5973 34 0 34 34 0 34 36 0 36 0 104

40636B2945 1F4A312640 11 0 11 11 0 11 11 0 11 0 33

40636B2945 7F7D487531 37 0 37 37 0 37 39 0 39 0 114

47037F460C 7F7D487531 37 0 37 37 0 37 37 0 37 0 112

4443240458 7F7E55466D 3 0 3 3 0 3 3 0 3 0 8

4443240458 431565767B 0 21 21 0 21 21 0 18 18 22 82

4443240458 132157621A 4 0 4 4 0 4 4 0 4 0 11

4443240458 115529097A 18 0 18 18 0 18 18 0 18 0 55

4443240458 1F4A3E1445 35 0 35 35 0 35 35 0 35 0 106

4443240458 115552116A 70 9 79 70 9 79 70 13 83 10 252

2007 TOTAL 6458 663 7121 6458 570 7028 6098 591 6689 622 21459

46


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


Garrison DamNFH

FRY

433843

1F4A36E66 115529097A 0 1805 1805 0 1805

1F4A36E66 115631222A 0 2375 2375 0 2375

1F4A36E66 115552116A 0 1378 1378 0 1378

115553544A 115556461A 0 6900 6900 0 6900

115553544A 4441774C6E 0 10904 10904 0 10904

470378405D 115556461A 0 7838 7838 0 7838

470378405D 1F4A34194A 0 7082 7082 0 7082

7F7B025D51 4441774C6E 0 14978 14978 0 14978

7F7B025D51 1F5001721E 0 23240 23240 0 23240

7F7B025D51 7F7D41431D 0 31663 31663 0 31663

47151A3D3A 1F4A363031 0 10179 10179 0 10179

47151A3D3A 7F7D372A6B 0 11250 11250 0 11250

47151A3D3A 115669294A 0 20456 20456 0 20456

40636B2945 4443422E34 0 6140 6140 0 6140

40636B2945 115712453A 0 34629 34629 0 34629

47037F460C 1F4A5A5A63 0 37712 37712 0 37712

47037F460C 454B30016B 0 66104 66104 0 66104

47037F460C 7F7F066471 0 37433 37433 0 37433

47


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


4443240458 132157621A 0 101777 101777 0 101777

2007 FRY TOTAL 0 434413 434413 0 434413

2008

Garrison DamNFH

115557463A 220E5E551E 265 0 265 265 64 329 0 156 750

115557463A 444334021A 204 0 204 204 66 270 0 162 636

115557463A 4310556551 332 0 332 332 66 398 0 160 890

115559532A 435F557624 199 0 199 199 68 267 0 163 629

115559532A 1F4A13592B 24 0 24 25 40 65 0 149 238

115559532A 444334021A 0 0 0 0 0 0 0 69 69

470A675627 7F7F066471 1382 0 1382 1549 54 1603 0 137 3122

470A675627 1F557B22071 1263 0 1263 1263 56 1319 0 136 2718

470A675627 115626162A 2006 0 2006 2022 56 2078 0 137 4221

1F497F1801 1F4849755B 431 0 431 431 54 485 0 137 1053

1F497F1801 7F7D2D723D 545 0 545 714 48 762 0 155 1462

1F497F1801 115712453A 930 0 930 951 55 1006 0 109 2045

1F497F1801 115626162A 0 0 0 0 6 6 0 17 23

1F497F1801 115667264A 8 0 8 8 8 16 0 18 42

MIX MIX 121 0 121 121 38 159 0 55 335

Elastomer MIX MIX 0 0 0 0 66 66 0 0 66

48


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


2008 TOTAL 7710 0 7710 8084 745 8829 0 0 0 1760 18299

FRY 470A675627 115626162A 0 0 Stocked @ Grand Champs 24395

2008 FRY TOTAL 0 0 0 0 24395


470468383C 1F4A13453F 0 31 31 67 30 97 72 72 200

470468383C 47037F3026 568 40 608 844 40 884 49 49 1541

470468383C 7F7B023408 332 38 370 300 38 338 54 54 762

454B490528 7F7D7C2447 0 0 0 0 87 87 87

454B490528 435F71414F 128 19 147 102 19 121 28 28 296

454B490528 44440A7B73 5 37 42 5 38 43 30 30 115

454B490528 1F477B3A65 297 56 353 305 56 361 57 57 771

115557165A 115679523A 623 46 669 450 46 496 23 23 1188

115557165A 7F7F06697C 319 31 350 451 31 482 34 34 866

115557165A 7F7D365422 970 33 1003 760 33 793 39 39 1835

115557165A 470A754E14 828 25 853 707 25 732 24 24 1609

7F7FD66963 465B6F1939 421 56 477 391 56 447 74 74 998

7F7FD66963 115525534A 938 38 976 946 38 984 123 123 2083

4315327C7B 7F7B082C10 47 30 77 143 38 181 48 48 306

4315327C7B 4718447879 32 0 32 42 0 42 142 142 216

49


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


4315327C7B 115626162A 35 35 38 38 43 43 116

220F01755C 7F7D37642C 1237 40 1277 762 39 801 34 34 2112

220F01755C 7F7B082208 833 40 873 775 40 815 42 42 1730

220F01755C 43105C602B 562 40 602 541 40 581 39 39 1222

2009 TOTAL 8140 635 8775 7591 645 8236 0 1042 1042 0 18053

Garrison DamNFH

FRY (11549)

115557165A 115679523A 0 0 0 0 2537 2537 0 0 0 2537

220F01755C 7F7D37642C 0 0 0 0 9012 9012 0 0 0 9012

2009 FRY TOTAL 0 0 0 0 11549 11549 0 0 0 0 11549


6C00024873 116165646A 0 66 66 0 65 65 0 0 0 0 131

6C00024873 1F4A24076C 0 74 74 0 75 75 0 0 0 0 149

1F5569653E 7F7D24754C 0 130 130 0 130 130 0 0 0 0 260

1F5569653E 132313521A 0 62 62 0 61 61 0 0 0 0 123

1F5569653E 1F4A33194B 125 135 260 125 137 262 0 0 0 0 522

43105F0C7E 44436E2975 0 57 57 0 60 60 0 0 0 0 117

43105F0C7E 1F4A143350 0 131 131 0 131 131 0 0 0 0 262

43105F0C7E 4704550E5B 125 140 265 125 138 263 0 0 0 0 528

2010 TOTAL 250 795 1045 250 797 1047 0 0 0 0 2092

2011 No stockings of 2011 year class occurred

50


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked



486A080614 2224076523 69 69 69 69 0 138

486A080614 7F7B024F2D 51 51 51 51 0 102

486A080614 7F7B025248 67 67 67 67 0 134

2012 TOTAL 0 187 187 0 187 187 0 0 0 0 374


462C7B2F49 1F4A435004 0 101 101 0 101 101 0 0 0 0 202

462C7B2F49 1F4A0C2E5D 0 0 0 0 0 0 0 0 0 0 0

462C7B2F49 1F4B225A1A 0 111 111 0 110 110 0 0 0 0 221

2013 TOTAL 0 212 212 0 211 211 0 0 0 0 423


7F7D517479 7F7D433241 0 62 62 0 62 62 0 0 0 0 124

7F7D517479 486838404F 0 100 100 0 100 100 0 0 0 0 200

2014 TOTAL 0 162 162 0 162 162 0 0 0 0 324


1F48421542 1F53312736 0 116 116 0 119 119 0 0 0 0 235

1F48421542 7F7D376F73 0 118 118 0 119 119 0 0 0 0 237

1F48421542 115556461A 0 119 119 0 119 119 0 0 0 0 238

2015 TOTAL 0 353 353 0 357 357 0 0 0 0 710

2016

Garrison DamNFH

462268474B 220E3F2578 0 100 100 0 100 100 0 0 0 0 200

462268474B 1F4772396F 0 8 8 0 8 8 0 0 0 0 16

462268474B 452738076 0 25 25 0 25 25 0 0 0 0 50

470378405D 220F0E6207 0 128 128 0 100 100 0 0 0 0 228

51


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


470378405D 411D0B4E09 0 29 29 0 29 29 0 0 0 0 58

4A46717168 7F7D23292E 0 100 100 0 100 100 0 0 0 0 200

7F7D517479 0A180E0E7E 0 99 99 0 100 100 0 0 0 0 199

7F7D517479 411D0B4E09 0 23 23 0 23 23 0 0 0 0 46

7F7D517479 7F7D7C2447 0 64 64 0 64 64 0 0 0 0 128

2016 TOTAL 0 576 576 0 549 549 0 0 0 0 1125

2017

Garrison DamNFH

7F7D7C2E4B 1F521B1E56 0 110 110 0 110 110 0 0 0 0 220

7F7D7C2E4B 115633183A 0 0 0 0 0 0 0 0 0 0 0

7F7D7C2E4B 115669540A 0 0 0 0 0 0 0 0 0 0 0

6C00040979 220F107A6F 0 0 0 0 0 0 0 0 0 0 0

6C00040979 7F7B024F2D 0 52 52 0 51 51 0 0 0 0 103

4A3E2D7E73 220E4E4E5D 0 26 26 0 26 26 0 0 0 0 52

4A49070E13 411D0E2C5F 0 18 18 0 18 18 0 0 0 0 36

4A49070E13 220E4E4E5D 0 47 47 0 47 47 0 0 0 0 94

4A49070E13 7F7E42795C 0 110 110 0 109 109 0 0 0 0 219

2017 TOTAL 0 363 363 0 361 361 0 0 0 0 724

2019

Garrison DamNFH

FRY

1F48421542 1F4A3E1445 0 0 0 0 131913 131913 0 0 0 0 131913

4315327C7B 1F4A3E1445 0 0 0 0 52565 52565 0 0 0 0 52565

4443250A24 1F4A3E1445 0 0 0 0 113526 113526 0 0 0 0 113526

52


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


4A49070E13 1F4A3E1445 0 0 0 0 110319 110319 0 0 0 0 110319

7F7D7C2E4B 1F4A3E1445 0 0 0 0 21888 21888 0 0 0 0 21888

46237D0338 4627764F65 0 0 0 0 17568 17568 0 0 0 0 17568

46255B170B 423F2F737D 0 0 0 27360 27360 0 0 0 27360

4628391106 4625703115 0 0 0 21735 21735 0 0 0 21735

46254E3E19 487F24652B 0 0 0 27306 27306 0 0 0 27306

44433E701A 46273A082B 0 0 0 33840 33840 0 0 0 33840

425708072B 4625537512 0 0 0 33579 33579 0 0 0 33579

46242E2A6F 462663187F 0 0 0 40014 40014 0 0 0 40014

4624485842 6C00096524 0 0 0 39312 39312 0 0 0 39312

444267561D 425669693F 0 0 0 43326 43326 0 0 0 43326

6C00090926 46256E7712 0 0 0 48222 48222 0 0 0 48222

4624127119 462616257F 0 0 0 9234 9234 0 0 0 9234

4624276027 0A14082E10 0 0 0 7560 7560 0 0 0 7560

42570A387A 1/1/00 0 0 0 39744 39744 0 0 0 39744

6C00093506 471C52622E 0 0 0 2228 2228 0 0 0 2228

1F4A125B2A 462446145B 0 0 0 10517 10517 0 0 0 10517

462546534B 4626214B69 0 0 0 19008 19008 0 0 0 19008

4627716D2D 1/1/00 0 0 0 15246 15246 0 0 0 15246

53


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


46257D6F35 423A314D00 0 0 0 33840 33840 0 0 0 33840

4627787556 6C00090633 0 0 0 4064 4064 0 0 0 4064

4624380D66 424F2F3D60 0 0 0 23220 23220 0 0 0 23220

6C00096611 471C52622E 0 0 0 12960 12960 0 0 0 12960

444175202F 423A314D05 0 0 0 0 32400 32400 0 0 0 32400

2019 FRY TOTAL 0 0 0 0 972494 972494 0 0 0 972494

GRAND TOTAL (Fry not included) 29872 5751 35623 29939 6524 36463 8545 6761 15306 2382 89773

54

Appendix 4. RPA 2 Yellowstone River Stockings from Garrison Dam NFH

Pallid Sturgeon Augmentation in the Recovery Area 2 Yellowstone River by Family

Year

Class

Hatchery Female

Pit tag

Male

Pit tag

Number stocked per stock site (Fall/Spring/Total) Summer

Stock

Totalstocked

Sidney Intake Fallon/Powder Forsyth/Kinsey


7F7F06672B 7F7D3C5708 - 0 0 - 0 0 - 0 0 - - 0 0

7F7F06672B 115631222A - 0 0 - 0 0 - 0 0 - - 0 0

411D262C1F 1F4A4B5973 - 85 85 - 13 13 - 85 85 - - 0 183

411D262C1F 411D0E2C5F - 86 86 - 0 0 - 85 85 - - 0 171

411D262C1F 41476A0462 - 85 85 - 84 84 - 85 85 - - 0 254

411D262C1F 411D0B4E09 - 85 85 - 0 0 - 21 21 - - 0 106

411D262C1F 17509415139 - 86 86 - 0 0 - 84 84 - - 0 170

220E345E09 1F4A111C6A - 0 0 - 0 0 - 0 0 - - 0 0

220E345E09 1F4A27214F - 0 0 - 0 0 - 0 0 - - 0 0

2001 TOTAL - 427 427 - 97 97 - 360 360 - - 0 884


114476216A 7F7D487531 75 - 75 - - 0 - - 0 - - 0 75

114476216A 1F4A4B5973 150 - 150 - - 0 - - 0 - - 0 150

114476216A 7F7E55466D 1382 - 1382 - - 0 - - 0 - - 0 1382

114476216A 1F4A312640 443 - 443 - - 0 - - 0 - - 0 443

114476216A 430E452777 941 - 941 482 - 482 - - 0 - - 0 1423

114476216A 1F477B3A65 - - 0 353 - 353 - - 0 - - 0 353

114476216A 431565767B - - 0 0 - 0 - - 0 - - 0 0

115555495A 431565767B - - 0 - - 0 - - 0 - - 0 0

55


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


115551683A 7F7D3C555D - - 0 107 - 107 - - 0 - - 0 107

7F7F066452 7F7F065834 - - 0 118 - 118 - - 0 - - 0 118

454910202B 1F47606357 - - 0 577 - 577 - - 0 - - 0 577

454910202B 220F0F7677 - - 0 172 - 172 - - 0 - - 0 172

454910202B 115679374A - - 0 293 - 293 - - 0 - - 0 293

454B380D60 7F7D376F73 - - 0 83 - 83 - - 0 - - 0 83

454B380D60 7F7F065834 - - 0 292 - 292 - - 0 - - 0 292

1F5330401E - - 0 - - 0 - - 0 - - 0 0

2004 TOTAL 2991 - 2991 2477 - 2477 - - 0 - - 0 5468

2005

Garrison DamNFH

7F7B026102 115553761A 109 67 176 522 200 722 - - 0 - - 0 898

7F7B026102 7F7B023253 0 63 63 0 190 190 - - 0 - - 0 253

115676635A 1F50072169 37 27 64 83 80 163 - - 0 - - 0 227

115676635A 7F7D2D723D 67 26 93 114 80 194 - - 0 - - 0 287

115676635A 7F7B031F17 84 26 110 194 80 274 - - 0 - - 0 384

115557165A 1F50072169 110 26 136 207 98 305 - - 0 - - 0 441

115557165A 7F7D2D723D 30 26 56 237 80 317 - - 0 - - 0 373

115557165A 7F7B031F17 111 26 137 263 80 343 - - 0 - - 0 480

4443240458 115633183A 105 26 131 229 68 297 - - 0 - - 0 428

4443240458 444334021A 109 0 109 175 14 189 - - 0 - - 0 298

RUNT MIX - 120 120 - 96 96 - - 0 - - 0 216

56


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


2005 TOTAL 762 433 1195 2024 1066 3090 - - 0 - - 0 4285


4443250A24 465D43745B 20 19 39 123 75 198 0 47 47 0 47 47 331

4443250A24 115529097A 6 17 23 15 66 81 0 41 41 0 41 41 186

4443250A24 435F7C1210 0 17 17 0 67 67 0 42 42 0 42 42 168

1F497F1801 1F4A3E1445 130 36 166 356 143 499 0 90 90 0 90 90 845

1F497F1801 466C473C59 78 36 114 201 145 346 0 90 90 0 91 91 641

1F497F1801 444171072D 77 36 113 196 145 341 0 90 90 0 90 90 634

1F497F1801 1F4A0B1A72 105 36 141 279 145 424 0 91 91 0 91 91 747

2006 TOTAL 416 197 613 1170 786 1956 0 491 491 0 492 492 3552


1F4A36E66 115529097A 52 2 54 204 9 213 130 5 135 63 5 68 26 496

1F4A36E66 115631222A 7 3 10 86 12 98 2 7 9 0 7 7 33 157

1F4A36E66 115552116A 4 0 4 16 16 32 10 10 20 10 10 20 49 125

115557165A 1F47760123 27 27 108 108 67 67 144 144 346

115557165A 431565767B 18 2 20 71 22 93 45 19 64 281 6 287 26 490

115557165A 7F7D291A07 27 1 28 107 4 111 67 2 69 76 2 78 13 298

115553544A 1F47652015 11 2 13 44 14 58 27 9 36 136 9 145 41 293

115553544A 115556461A 46 0 46 185 0 185 116 1 117 116 0 116 7 471

115553544A 4441774C6E 38 3 41 154 7 161 95 4 99 77 4 81 22 404

454B490528 1F48472D25 0 0 2 2 1 1 0 0 3

454B490528 424E36510D 16 1 17 66 6 72 41 4 45 0 4 4 20 158

57


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


454B490528 4714560E0E 15 0 15 60 6 66 37 11 48 46 10 56 44 229

470378405D 115556461A 45 45 180 180 113 113 22 22 360

470378405D 1F4A34194A 46 2 48 182 4 186 114 2 116 114 2 116 13 479

470378405D 1F4B26036D 106 0 106 425 19 444 266 12 278 266 12 278 52 1158

7F7B025D51 4441774C6E 44 4 48 176 7 183 110 5 115 109 5 114 21 480

7F7B025D51 1F5001721E 77 4 81 307 16 323 192 10 202 200 10 210 44 860

7F7B025D51 7F7D41431D 44 2 46 177 6 183 110 5 115 110 5 115 22 481

47151A3D3A 1F4A363031 24 7 31 98 59 157 61 14 75 0 15 15 67 345

47151A3D3A 7F7D372A6B 103 4 107 413 15 428 258 9 267 39 9 48 40 890

47151A3D3A 115669294A 165 6 171 662 22 684 413 14 427 167 14 181 60 1523

7F7FD66963 115525534A 66 7 73 266 24 290 165 17 182 371 17 388 73 1007

7F7FD66963 1F477B4E51 137 6 143 551 24 575 343 15 358 1147 15 1162 60 2298

7F7FD66963 220E5F4928 81 15 96 322 15 337 201 9 210 0 9 9 40 692

40636B2945 4443422E34 50 4 54 202 12 214 126 10 136 126 10 136 42 583

40636B2945 44440A7B73 13 13 53 53 33 33 35 35 135

40636B2945 115712453A 112 0 112 449 0 449 280 7 287 0 7 7 42 897

454B380D60 2204583665 143 0 143 571 0 571 357 15 372 254 15 269 60 1415

454B380D60 132114552A 7 2 9 29 7 36 18 4 22 19 4 23 22 112

454B380D60 7F7D4A7758 12 12 49 49 31 31 28 28 120

47037F460C 1F4A5A5A63 96 7 103 386 27 413 241 17 258 151 17 168 72 1014

58


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


47037F460C 454B30016B 13 13 52 52 32 32 39 39 136

47037F460C 7F7F066471 25 4 29 101 16 117 63 10 73 0 10 10 42 272

4443240458 1F4A206A0D 88 5 93 353 20 373 221 12 233 538 12 550 53 1302

4443240458 132157621A 92 6 98 368 25 393 230 16 246 485 16 501 63 1300

4443240458 466C473C59 0 0 0 0 0 0 0 0 0

115676635A 1F4A3E1445 9 9 37 37 23 23 0 0 69

7F7FD66963 115552116A 0 0 0 0 0 0 0 0 0

7F7FD66963 115529097A 0 0 0 0 0 0 0 0 0

40636B2945 1F4A4B5973 10 10 41 41 25 25 37 37 113

40636B2945 1F4A312640 3 3 13 13 8 8 10 10 34

40636B2945 7F7D487531 11 11 45 45 28 28 10 10 94

47037F460C 7F7D487531 11 11 45 45 28 28 28 28 112

4443240458 7F7E55466D 1 1 3 3 2 2 5 5 11

4443240458 431565767B 0 4 4 0 16 16 0 10 10 0 10 10 44 84

4443240458 132157621A 1 1 4 4 3 3 7 7 15

4443240458 115529097A 6 6 22 22 14 14 13 13 54

4443240458 1F4A3E1445 11 11 42 42 26 26 40 40 119

4443240458 115552116A 21 2 23 85 7 92 53 4 57 51 4 55 20 247

2007 TOTAL 1937 105 2042 7808 437 8245 4827 289 5116 5370 275 5645 1233 22282

59


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


Garrison DamNFH

FRY

(468,281)

115557165A 1F47760123 0 0 0 0 680 680 0 0 0 0 0 0 680

115557165A 7F7D291A07 0 0 0 0 386 386 0 0 0 0 0 0 386

115553544A 115556461A 0 0 0 0 1255 1255 0 0 0 0 0 0 1255

470378405D 115556461A 0 0 0 0 0 0 0 1337 1337 0 0 0 1337

470378405D 1F4A34194A 0 0 0 0 0 0 0 662 662 0 0 0 662

7F7B025D51 4441774C6E 0 0 0 0 0 0 0 657 657 0 0 0 657

7F7B025D51 7F7D41431D 0 0 0 0 0 0 0 790 790 0 0 0 790

7F7FD66963 1F477B4E51 0 0 0 0 2390 2390 0 0 0 0 0 0 2390

7F7FD66963 220E5F4928 0 0 0 0 9791 9791 0 0 0 0 0 0 9791

40636B2945 44440A7B73 0 0 0 0 0 0 0 1285 1285 0 0 0 1285

454B380D60 7F7D4A7758 0 0 0 0 0 0 0 6696 6696 0 0 0 6696

47037F460C 454B30016B 0 0 0 0 0 0 0 424 424 0 0 0 424

47151A3D3A CRYO MIX 0 0 0 0 1648 1648 0 0 0 0 0 0 1648

7F7F7D6693 CRYO MIX 0 0 0 0 2536 2536 0 0 0 0 0 0 2536

40636B2945 7F7D487531 0 0 0 0 2077 2077 0 0 0 0 0 0 2077

47037F460C 7F7D487531 0 0 0 0 0 0 0 1824 1824 0 0 0 1824

2007 FRY TOTAL 0 0 0 0 20763 20763 0 13675 13675 0 0 0 34438


115557463A 220E5E551E 53 0 53 213 0 213 411 0 411 133 13 146 158 981

115557463A 444334021A 41 7 48 165 26 191 126 16 142 102 16 118 159 658

115557463A 4310556551 66 7 73 383 26 409 343 35 378 166 16 182 142 1184

115559532A 435F557624 40 7 47 152 27 179 143 17 160 1220 17 1237 163 1786

60


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


115559532A 1F4A13592B 5 6 11 17 24 41 12 15 27 12 15 27 144 250

115559532A 444334021A 0 3 3 0 7 7 0 7 7 0 7 7 74 98

470A675627 7F7F066471 309 6 315 2287 23 2310 773 14 787 775 14 789 138 4339

470A675627 1F557B22071 252 6 258 932 0 932 632 14 646 629 14 643 128 2607

470A675627 115626162A 392 6 398 1631 21 1652 823 14 837 987 14 1001 128 4016

1F497F1801 1F4849755B 86 6 92 310 22 332 216 14 230 216 14 230 149 1033

1F497F1801 7F7D2D723D 142 5 147 497 16 513 357 12 369 357 12 369 177 1575

1F497F1801 115712453A 179 6 185 563 22 585 132 14 146 285 14 299 135 1350

1F497F1801 115626162A 0 1 1 0 3 3 0 2 2 0 2 2 18 26

1F497F1801 115667264A 2 1 3 8 3 11 17 2 19 4 2 6 17 56

MIX MIX 24 4 28 261 0 261 61 9 70 61 9 70 48 477

Elastomer MIX MIX 0 215 215 0 64 0 0 0 0 0 0 0 0 215

2008 TOTAL 1591 286 1877 7419 284 7703 4046 185 4231 4947 179 5126 1778 20715

Garrison Dam

NFH fry

(4,797)

470A675627 1F557B22071 Stocked @ Tongue Confluence 2974

1F497F1801 7F7D2D723D Stocked @ Tongue Confluence 1823

2008 FRY TOTAL 4797

61


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


2009 GarrisonDam NFH

470468383C 1F4A13453F 16 14 30 63 55 118 40 35 75 56 35 91 0 314

470468383C 47037F3026 168 13 181 676 52 728 423 32 455 423 34 457 0 1821

470468383C 7F7B023408 82 12 94 328 49 377 205 31 236 205 31 236 0 943

454B490528 7F7D7C2447 6 6 23 23 14 14 14 14 57

454B490528 435F71414F 30 7 37 121 29 150 75 18 93 75 18 93 0 373

454B490528 44440A7B73 1 11 12 4 46 50 3 29 32 3 29 32 0 126

454B490528 1F477B3A65 17 17 34 69 70 139 43 44 87 43 44 87 0 347

115557165A 115679523A 119 11 130 479 46 525 300 29 329 300 29 329 0 1313

115557165A 7F7F06697C 90 10 100 360 40 400 225 25 250 225 25 250 0 1000

115557165A 7F7D365422 156 10 166 608 41 649 380 26 406 380 26 406 0 1627

115557165A 470A754E14 141 8 149 566 30 596 354 19 373 354 19 373 0 1491

7F7FD66963 465B6F1939 91 18 109 365 70 435 228 44 272 228 44 272 0 1088

7F7FD66963 115525534A 166 21 187 666 85 751 417 53 470 417 53 470 0 1878

4315327C7B 7F7B082C10 29 14 43 115 55 170 72 35 107 72 35 107 0 427

4315327C7B 4718447879 8 14 22 34 54 88 21 34 55 21 34 55 0 220

4315327C7B 115626162A 12 12 48 48 30 30 30 30 120

220F01755C 7F7D37642C 153 12 165 614 48 662 384 30 414 384 30 414 0 1655

220F01755C 7F7B082208 154 13 167 617 50 667 386 32 418 386 32 418 0 1670

220F01755C 43105C602B 155 12 167 624 48 672 390 30 420 390 30 420 0 1679

2009 TOTAL 1576 235 1811 6309 939 7248 3946 590 4536 3962 592 4554 0 18149

62


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


Garrison DamNFH

FRY (21,428)

7F7FD66963 220E4E4E5D 4177

115557165A 470A754E14 1768

115557165A 7F7D365422 2078

115557165A 7F7F06697C 428

220F01755C 7F7B082208 11612

220F01755C 43105C602B 3242

2009 FRY TOTAL Fry stocked at Miles City 23305


6C00024873 116165646A 0 0 0 44 44 0 44 44 0 55 55 0 143

6C00024873 1F4A24076C 0 0 0 51 51 0 56 56 0 40 40 0 147

1F5569653E 7F7D24754C 0 0 0 61 61 0 86 86 0 125 125 0 272

1F5569653E 132313521A 0 0 0 41 41 0 39 39 0 40 40 0 120

1F5569653E 1F4A33194B 0 0 125 92 217 125 90 215 0 89 89 0 521

43105F0C7E 44436E2975 0 0 0 39 39 0 39 39 0 36 36 0 114

43105F0C7E 1F4A143350 0 0 0 88 88 0 86 86 0 67 67 0 241

43105F0C7E 4704550E5B 0 0 125 94 219 125 91 216 0 93 93 0 528

2010 TOTAL 0 0 0 250 510 760 250 531 781 0 545 545 0 2086

2011 No stockings of 2011 year class occurred 0 0


486A080614 2224076523 0 0 0 0 65 65 0 69 69 0 0 0 0 134

486A080614 7F7B024F2D 0 0 0 0 51 51 0 51 51 0 0 0 0 102

486A080614 7F7B025248 0 0 0 0 67 67 0 67 67 0 0 0 0 134

2012TOTAL 0 0 0 0 183 183 0 187 187 0 0 0 0 370

63


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked



462C7B2F49 1F4A435004 0 0 0 0 0 0 0 100 100 0 101 101 0 201

1F4A0C2E5D 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1F4B225A1A 0 0 0 0 0 0 0 110 110 0 113 113 0 223

2013 TOTAL 0 0 0 0 0 0 0 210 210 0 214 214 0 424


7F7D517479 7F7D433241 0 0 0 0 0 0 0 62 62 0 62 62 0 124

7F7D517479 486838404F 0 0 0 0 0 0 0 100 100 0 100 100 0 200

2014 TOTAL 0 0 0 0 0 0 0 162 162 0 162 162 0 324


1F48421542 1F53312736 0 0 0 0 119 119 0 119 119 0 0 0 0 238

1F48421542 7F7D376F73 0 0 0 0 119 119 0 119 119 0 0 0 0 238

1F48421542 115556461A 0 0 0 0 119 119 0 119 119 0 0 0 0 238

2015 TOTAL 0 0 0 0 357 357 0 357 357 0 0 0 0 714

2016

Garrison DamNFH

462268474B 220E3F2578 0 0 0 0 100 100 0 100 100 0 0 0 0 200

462268474B 1F4772396F 0 0 0 0 7 7 0 7 7 0 0 0 0 14

462268474B 452738076 0 0 0 0 24 24 0 25 25 0 0 0 0 49

470378405D 220F0E6207 0 0 0 0 100 100 0 100 100 0 0 0 0 200

470378405D 411D0B4E09 0 0 0 0 29 29 0 28 28 0 0 0 0 57

4A46717168 7F7D23292E 0 0 0 0 100 100 0 100 100 0 0 0 0 200

7F7D517479 0A180E0E7E 0 0 0 0 100 100 0 100 100 0 0 0 0 200

7F7D517479 411D0B4E09 0 0 0 0 24 24 0 38 38 0 0 0 0 62

7F7D517479 7F7D7C2447 0 0 0 0 58 58 0 64 64 0 0 0 0 122

2016 TOTAL 0 0 0 0 542 542 0 562 562 0 0 0 0 1104

64


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Stock

Totalstocked


2017

Garrison DamNFH

7F7D7C2E4B 1F521B1E56 0 0 0 0 110 110 0 110 110 0 0 0 0 220

7F7D7C2E4B 115633183A 0 0 0 0 0 0 0 0 0 0 0 0 0 0

7F7D7C2E4B 115669540A 0 0 0 0 0 0 0 0 0 0 0 0 0 0

6C00040979 220F107A6F 0 0 0 0 0 0 0 0 0 0 0 0 0 0

6C00040979 7F7B024F2D 0 0 0 0 52 52 0 52 52 0 0 0 0 104

4A3E2D7E73 220E4E4E5D 0 0 0 0 26 26 0 26 26 0 0 0 0 52

4A49070E13 411D0E2C5F 0 0 0 0 18 18 0 18 18 0 0 0 0 36

4A49070E13 220E4E4E5D 0 0 0 0 47 47 0 47 47 0 0 0 0 94

4A49070E13 7F7E42795C 0 0 0 0 108 108 0 110 110 0 0 0 0 218

2017 TOTAL 0 0 0 0 361 361 0 363 363 0 0 0 0 724

2019 No stockings of 2019 year class occurred 0

2020

Garrison DamNFH

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0

2020 TOTAL 0 0 0 0 0 0 0 0 0 0 0 0 0 0

GRAND TOTAL (Fry not included) 9273 1683 10956 27457 5562 33019 13069 4287 17356 14279 2459 16738 3011 81081

65

Appendix 5. RPA 1 Stockings from Garrison Dam NFH

Pallid Sturgeon Augmentation in the Recovery Area 1 by Family

Year

Class

Hatchery Female

Pit tag

Male

Pit tag

Number stocked per stock site (Fall/Spring/Total) Totalstocked

Robinson Loma Coal Banks Judith


4443250A24 465D43745B - 107 107 - 106 106 - 106 106 - 106 106 425

4443250A24 115529097A - 94 94 - 94 94 - 94 94 - 94 94 376

4443250A24 435F7C1210 - 102 102 - 96 96 - 97 97 - 97 97 392

1F497F1801 1F4A3E1445 - 214 214 - 206 206 - 204 204 - 204 204 828

1F497F1801 466C473C59 - 207 207 - 206 206 - 200 200 - 205 205 818

1F497F1801 444171072D - 210 210 - 206 206 - 206 206 - 206 206 828

1F497F1801 1F4A0B1A72 - 214 214 - 209 209 - 207 207 - 207 207 837

GAP GAP - 7 7 0 0 0 0 0 0 7

TOTAL - 1155 1155 - 1123 1123 - 1114 1114 - 1119 1119 4511


1F4A36E66 115529097A 22 13 35 22 13 35 22 13 35 22 13 35 140

1F4A36E66 115631222A 3 18 21 3 18 21 3 18 21 3 18 21 84

1F4A36E66 115552116A 2 25 27 2 25 27 2 27 29 2 23 25 108

115557165A 1F47760123 11 0 11 11 0 11 11 0 11 11 0 11 44

115557165A 431565767B 8 14 22 8 14 22 8 14 22 8 14 22 88

115557165A 7F7D291A07 11 6 17 11 6 17 11 6 17 11 6 17 68

115553544A 1F47652015 5 22 27 5 22 27 5 22 27 5 22 27 108

115553544A 115556461A 20 2 22 20 2 22 20 3 23 20 1 21 88

115553544A 4441774C6E 16 11 27 16 11 27 16 11 27 16 11 27 108

454B490528 1F48472D25 0 0 0 0 0 0 0 0 0 0 0 0 0

66


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



454B490528 424E36510D 7 10 17 7 10 17 7 10 17 7 10 17 68

454B490528 4714560E0E 6 23 29 6 17 23 6 34 40 6 22 28 120

470378405D 115556461A 19 0 19 19 0 19 19 0 19 19 0 19 76

470378405D 1F4A34194A 19 6 25 19 6 25 19 6 25 19 6 25 100

470378405D 1F4B26036D 45 30 75 45 30 75 45 30 75 45 30 75 300

7F7B025D51 4441774C6E 19 11 30 19 11 30 19 11 30 19 11 30 120

7F7B025D51 1F5001721E 33 25 58 33 25 58 33 25 58 33 25 58 232

7F7B025D51 7F7D41431D 19 11 30 19 10 29 19 12 31 19 11 30 120

47151A3D3A 1F4A363031 10 36 46 10 38 48 10 40 50 10 38 48 192

47151A3D3A 7F7D372A6B 44 23 67 44 19 63 44 28 72 72 22 94 296

47151A3D3A 115669294A 70 36 106 70 36 106 70 36 106 70 36 106 424

7F7FD66963 115525534A 28 43 71 28 41 69 28 45 73 28 43 71 284

7F7FD66963 1F477B4E51 58 37 95 58 35 93 58 39 97 58 37 95 380

7F7FD66963 220E5F4928 34 23 57 34 23 57 34 23 57 34 23 57 228

40636B2945 4443422E34 21 24 45 21 24 45 21 25 46 21 23 44 180

40636B2945 44440A7B73 6 0 6 6 0 6 6 0 6 6 0 6 24

40636B2945 115712453A 48 11 59 48 14 62 48 28 76 48 15 63 260

454B380D60 2204583665 61 37 98 61 34 95 61 43 104 61 34 95 392

454B380D60 132114552A 3 11 14 3 10 13 3 12 15 3 11 14 56

454B380D60 7F7D4A7758 5 0 5 5 0 5 5 0 5 5 0 5 20

47037F460C 1F4A5A5A63 41 42 83 41 42 83 41 42 83 41 42 83 332

67


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



47037F460C 454B30016B 6 0 6 6 0 6 6 0 6 6 0 6 24

47037F460C 7F7F066471 11 25 36 11 25 36 11 25 36 11 25 36 144

4443240458 1F4A206A0D 38 31 69 38 31 69 38 31 69 38 31 69 276

4443240458 132157621A 39 39 78 39 39 78 39 39 78 39 39 78 312

4443240458 466C473C59 0 0 0 0 0 0 0 0 0 0 0 0 0

115676635A 1F4A3E1445 5 0 5 4 0 4 4 0 4 4 0 4 17

7F7FD66963 115552116A 0 0 0 0 0 0 0 0 0 0 0 0 0

7F7FD66963 115529097A 0 0 0 0 0 0 0 0 0 0 0 0 0

40636B2945 1F4A4B5973 4 0 4 4 0 4 4 0 4 4 0 4 16

40636B2945 1F4A312640 1 0 1 1 0 1 1 0 1 1 0 1 4

40636B2945 7F7D487531 5 0 5 5 0 5 5 0 5 5 0 5 20

47037F460C 7F7D487531 5 0 5 5 0 5 5 0 5 5 0 5 20

4443240458 7F7E55466D 0 0 0 0 0 0 0 0 0 0 0 0 0

4443240458 431565767B 0 26 26 0 26 26 0 26 26 0 26 26 104

4443240458 132157621A 0 0 0 0 0 0 0 0 0 0 0 0 0

4443240458 115529097A 2 0 2 2 0 2 2 0 2 2 0 2 8

4443240458 1F4A3E1445 4 0 4 4 0 4 4 0 4 4 0 4 16

4443240458 115552116A 9 11 20 9 11 20 9 11 20 9 11 20 80

TOTAL 823 682 1505 822 668 1490 822 735 1557 850 679 1529 6081


115557463A 220E5E551E 150 0 150 166 129 295 165 104 269 165 104 269 983

115557463A 444334021A 127 100 227 127 109 236 128 109 237 127 109 236 936

68


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



115557463A 4310556551 207 7 214 207 108 315 207 106 313 207 108 315 1157

115559532A 435F557624 124 26 150 124 110 234 124 111 235 130 111 241 860

115559532A 1F4A13592B 15 5 20 15 101 116 15 101 116 9 101 110 362

115559532A 444334021A 0 0 0 0 48 48 0 18 18 0 48 48 114

470A675627 7F7F066471 964 68 1032 724 92 816 964 93 1057 609 94 703 3608

470A675627 1F557B22071 786 11 797 611 91 702 786 91 877 786 91 877 3253

470A675627 115626162A 1228 85 1313 1430 93 1523 1228 94 1322 1228 92 1320 5478

1F497F1801 1F4849755B 268 108 376 102 67 169 268 92 360 268 92 360 1265

1F497F1801 7F7D2D723D 445 27 472 445 78 523 445 78 523 445 78 523 2041

1F497F1801 115712453A 592 50 642 279 91 370 592 91 683 592 91 683 2378

1F497F1801 115626162A 0 11 11 0 12 12 0 11 11 0 12 12 46

1F497F1801 115667264A 0 9 9 0 12 12 5 10 15 5 12 17 53

MIX MIX 75 0 75 51 61 112 75 9 84 75 62 137 408

Elastomer MIX MIX 0 549 549 0 0 0 0 0 0 0 0 0 549

TOTAL 4981 1056 6037 4281 1202 5483 5002 1118 6120 4646 1205 5851 23491

FRY 470A675627 7F7F066471 0 32152 0 0 32152

2008 FRY TOTAL 0 32152 0 0 32152

69


Year

Class

Hatchery Female

Pit tag

Male

Pit tag




470468383C 1F4A13453F 99 99 158 65 223 63 63 63 63 448

470468383C 47037F3026 1053 1053 1049 59 1108 59 59 59 59 2279

470468383C 7F7B023408 511 511 512 58 570 58 58 58 58 1197

454B490528 7F7D7C2447 0 0 0 27 27 26 23 27

454B490528 435F71414F 188 188 193 34 227 33 33 33 33 481

454B490528 44440A7B73 7 7 7 53 60 53 53 53 53 173

454B490528 1F477B3A65 108 108 111 81 192 82 82 82 82 464

115557165A 115679523A 746 746 748 53 801 54 54 54 54 1655

115557165A 7F7F06697C 559 559 560 47 607 46 46 46 46 1258

115557165A 7F7D365422 946 946 950 47 997 47 47 47 47 2037

115557165A 470A754E14 881 881 881 36 917 35 35 35 35 1868

7F7FD66963 465B6F1939 567 567 567 81 648 82 82 82 82 1379

7F7FD66963 115525534A 1036 1036 1036 98 1134 98 98 98 98 2366

4315327C7B 7F7B082C10 179 179 138 63 201 64 64 60 60 504

4315327C7B 4718447879 14 14 92 63 155 63 63 63 63 295

4315327C7B 115626162A 0 0 0 57 57 56 56 56 56 169

220F01755C 7F7D37642C 954 954 954 57 1011 58 58 58 58 2081

220F01755C 7F7B082208 961 961 961 56 1017 58 58 58 58 2094

220F01755C 43105C602B 971 971 971 56 1027 56 56 56 56 2110

2009 TOTAL 9780 0 9780 9888 1091 10979 0 1091 1065 0 1084 1084 22908

70


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



Garrison DamNFH

FRY (21,428)

115557165A 7F7F06697C 0

115557165A 115679523A 0

115557165A 7F7D365422 0

115557165A 470A75E414 0

2009 FRY TOTAL Larval Drift stocking immediately above Fort Peck Reservoir 0


6C00024873 116165646A 0 47 47 0 47 47 0 0 0 0 0 0 94

6C00024873 1F4A24076C 0 47 47 0 47 47 0 0 0 0 0 0 94

1F5569653E 7F7D24754C 0 47 47 0 47 47 0 0 0 0 0 0 94

1F5569653E 132313521A 0 47 47 0 47 47 0 0 0 0 0 0 94

1F5569653E 1F4A33194B 125 47 172 125 47 172 0 0 0 0 0 0 344

43105F0C7E 44436E2975 0 47 47 0 47 47 0 0 0 0 0 0 94

43105F0C7E 1F4A143350 0 47 47 0 47 47 0 0 0 0 0 0 94

43105F0C7E 4704550E5B 125 47 172 125 47 172 0 0 0 0 0 0 344

2010 TOTAL 250 376 626 250 376 626 0 0 0 0 0 0 1252



486A080614 2224076523 0 87 87 0 87 87 0 0 0 0 0 0 174

486A080614 7F7B024F2D 0 63 63 0 63 63 0 0 0 0 0 0 126

486A080614 7F7B025248 0 82 82 0 83 83 0 0 0 0 0 0 165

2012TOTAL 0 232 232 0 233 233 0 0 0 0 0 0 465


462C7B2F49 1F4A435004 0 0 0 0 0 0 0 0 0 0 0 0 0

1F4A0C2E5D 0 0 0 0 0 0 0 0 0 0 0 0 0

71


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



1F4B225A1A 606 160 766 0 159 159 0 0 0 0 0 0 925

2013 TOTAL 606 160 766 0 159 159 0 0 0 0 0 0 925


7F7D517479 7F7D433241 0 113 113 0 112 112 0 0 0 0 0 0 225

7F7D517479 486838404F 342 150 342 0 150 150 0 0 0 0 0 0 492

2014 TOTAL 342 113 455 0 262 262 0 0 0 0 0 0 717


462268474B 220E3F2578 0 170 170 0 164 164 0 0 0 0 0 0 334

462268474B 1F4772396F 0 15 15 0 15 15 0 0 0 0 0 0 30

462268474B 1/1/00 0 44 44 0 44 44 0 0 0 0 0 0 88

470378405D 220F0E6207 0 140 140 0 147 147 0 0 0 0 0 0 287

470378405D 411D0B4E09 0 113 113 0 121 121 0 0 0 0 0 0 234

4A46717168 7F7D23292E 0 162 162 0 164 164 0 0 0 0 0 0 326

4A46717168 7F7F065834 0 69 69 0 69 69 0 0 0 0 0 0 138

7F7D517479 0A180E0E7E 0 165 165 0 164 164 0 0 0 0 0 0 329

7F7D517479 7F7D7C2447 0 115 115 0 116 116 0 0 0 0 0 0 231

7F7FD66963 132313521A 0 69 69 0 70 70 0 0 0 0 0 0 139

2016 TOTAL 0 1062 1062 0 1074 1074 0 0 0 0 0 0 2136


7F7D7C2E4B 1F521B1E56 0 149 149 0 148 148 0 0 0 0 0 0 297

7F7D7C2E4B 115633183A 0 9 9 0 9 9 0 0 0 0 0 0 18

7F7D7C2E4B 115669540A 0 148 148 0 149 149 0 0 0 0 0 0 297

6C00040979 220F107A6F 0 122 122 0 122 122 0 0 0 0 0 0 244

72


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



6C00040979 7F7B024F2D 0 78 78 0 79 79 0 0 0 0 0 0 157

4A3E2D7E73 220E4E4E5D 0 50 50 0 50 50 0 0 0 0 0 0 100

4A49070E13 411D0E2C5F 0 91 91 0 92 92 0 0 0 0 0 0 183

4A49070E13 220E4E4E5D 10600 63 10663 0 61 61 0 0 0 0 0 0 10724

4A49070E13 7F7E42795C 0 149 149 0 147 147 0 0 0 0 0 0 296

2017 TOTAL 10600 859 11459 0 857 857 0 0 0 0 0 0 12316



0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

2020 TOTAL 0 0 0 0 0 0 0 0 0 0 0 0 0

GRAND TOTAL (Fry not included) 27132 5319 32451 14991 6669 21660 5824 4058 9856 5496 4087 9583 73550

73



Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked

Verdel Standing Bear/Running Water


7F7F06672B 7F7D3C5708 0 67 67 0 0 0 0 67

7F7F06672B 115631222A 0 71 71 0 0 0 0 71

411D262C1F 1F4A4B5973 0 70 70 0 0 0 0 70

411D262C1F 411D0E2C5F 0 70 70 0 0 0 0 70

411D262C1F 41476A0462 0 70 70 0 0 0 0 70

411D262C1F 411D0B4E09 0 0 0 0 0 0 0 0

411D262C1F 17509415139 0 70 70 0 0 0 0 70

220E345E09 1F4A111C6A 0 70 70 0 0 0 0 70

220E345E09 1F4A27214F 0 70 70 0 0 0 0 70

2001 TOTAL 0 558 558 0 0 0 0 558

2007 Garrison Dam 1F4A36E66 115529097A 0 0 0 0 0 0 20 20

1F4A36E66 115631222A 0 0 0 0 0 0 20 20

1F4A36E66 115552116A 0 0 0 0 0 0 20 20

115557165A 1F47760123 0 0 0 0 0 0 0 0

115557165A 431565767B 0 0 0 0 0 0 20 20

115557165A 7F7D291A07 0 0 0 0 0 0 20 20

74


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


115553544A 1F47652015 0 0 0 0 0 0 20 20

115553544A 115556461A 0 0 0 0 0 0 20 20

115553544A 4441774C6E 0 0 0 0 0 0 20 20

454B490528 1F48472D25 0 0 0 0 0 0 0 0

454B490528 424E36510D 0 0 0 0 0 0 20 20

454B490528 4714560E0E 0 0 0 0 0 0 20 20

470378405D 115556461A 0 0 0 0 0 0 0 0

470378405D 1F4A34194A 0 0 0 0 0 0 20 20

470378405D 1F4B26036D 0 0 0 0 0 0 19 19

7F7B025D51 4441774C6E 0 0 0 0 0 0 19 19

7F7B025D51 1F5001721E 0 0 0 0 0 0 19 19

7F7B025D51 7F7D41431D 0 0 0 0 0 0 19 19

47151A3D3A 1F4A363031 0 0 0 0 0 0 19 19

47151A3D3A 7F7D372A6B 0 0 0 0 0 0 19 19

47151A3D3A 115669294A 0 0 0 0 0 0 20 20

7F7FD66963 115525534A 0 0 0 0 0 0 19 19

7F7FD66963 1F477B4E51 0 0 0 0 0 0 19 19

7F7FD66963 220E5F4928 0 0 0 0 0 0 19 19

40636B2945 4443422E34 0 0 0 0 0 0 19 19

75


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


40636B2945 44440A7B73 0 0 0 0 0 0 0 0

40636B2945 115712453A 0 0 0 0 0 0 19 19

454B380D60 2204583665 0 0 0 0 0 0 19 19

454B380D60 132114552A 0 0 0 0 0 0 19 19

454B380D60 7F7D4A7758 0 0 0 0 0 0 0 0

47037F460C 1F4A5A5A63 0 0 0 0 0 0 19 19

47037F460C 454B30016B 0 0 0 0 0 0 0 0

47037F460C 7F7F066471 0 0 0 0 0 0 19 19

4443240458 1F4A206A0D 0 0 0 0 0 0 19 19

4443240458 132157621A 0 0 0 0 0 0 0 0

4443240458 466C473C59 0 0 0 0 0 0 0 0

115676635A 1F4A3E1445 0 0 0 0 0 0 0 0

7F7FD66963 115552116A 0 0 0 0 0 0 0 0

7F7FD66963 115529097A 0 0 0 0 0 0 0 0

40636B2945 1F4A4B5973 0 0 0 0 0 0 0 0

40636B2945 1F4A312640 0 0 0 0 0 0 0 0

40636B2945 7F7D487531 0 0 0 0 0 0 0 0

47037F460C 7F7D487531 0 0 0 0 0 0 0 0

4443240458 7F7E55466D 0 0 0 0 0 0 0 0

76


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


4443240458 431565767B 0 0 0 0 0 0 19 19

4443240458 132157621A 0 0 0 0 0 0 19 19

4443240458 115529097A 0 0 0 0 0 0 0 0

4443240458 1F4A3E1445 0 0 0 0 0 0 0 0

4443240458 115552116A 0 0 0 0 0 0 19 19

2007 TOTAL 0 0 0 0 0 0 601 601


115557463A 220E5E551E 0 25 25 0 0 0 0 25

115557463A 444334021A 0 24 24 0 0 0 0 24

115557463A 4310556551 0 26 26 0 0 0 0 26

115559532A 435F557624 0 0 0 0 0 0 0 0

115559532A 1F4A13592B 0 27 27 0 0 0 0 27

115559532A 444334021A 0 13 13 0 0 0 0 13

115559532A 7F7D437520 0 30 30 0 0 0 0 30

470A675627 7F7F066471 0 25 25 0 0 0 0 25

470A675627 1F557B22071 0 0 0 0 0 0 0 0

470A675627 115626162A 0 24 24 0 0 0 0 24

470A675627 7F7E55466D 0 19 19 0 0 0 0 19

1F497F1801 1F4849755B 0 25 25 0 0 0 0 25

77


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


1F497F1801 7F7D2D723D 0 0 0 0 0 0 0 0

1F497F1801 115712453A 0 24 24 0 0 0 0 24

1F497F1801 115626162A 0 3 3 0 0 0 0 3

1F497F1801 115667264A 0 0 0 0 0 0 0 0

1F497F1801 7F7F066471 0 4 4 0 0 0 0 4

MIX MIX 0 28 28 0 0 0 0 28

2008 TOTAL 0 297 297 0 0 0 0 297


470468383C 1F4A13453F 0 25 25 0 0 0 0 25

470468383C 47037F3026 0 23 23 0 0 0 0 23

470468383C 7F7B023408 0 22 22 0 0 0 0 22

454B490528 7F7D7C2447 0 0 0 0 0 0 0 0

454B490528 435F71414F 0 27 27 0 0 0 0 27

454B490528 44440A7B73 0 26 26 0 0 0 0 26

454B490528 1F477B3A65 0 42 42 0 0 0 0 42

115557165A 115679523A 0 21 21 0 0 0 0 21

115557165A 7F7F06697C 0 18 18 0 0 0 0 18

115557165A 7F7D365422 0 14 14 0 0 0 0 14

115557165A 470A754E14 0 14 14 0 0 0 0 14

78


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


7F7FD66963 465B6F1939 0 31 31 0 0 0 0 31

7F7FD66963 115525534A 0 38 38 0 0 0 0 38

4315327C7B 7F7B082C10 0 25 25 0 0 0 0 25

4315327C7B 4718447879 0 0 0 0 0 0 0 0

4315327C7B 115626162A 0 22 22 0 0 0 0 22

220F01755C 7F7D37642C 0 75 75 0 0 0 0 75

220F01755C 7F7B082208 0 28 28 0 0 0 0 28

220F01755C 43105C602B 0 43 43 0 0 0 0 43

2009 TOTAL 0 494 494 0 0 0 0 494


7F7D517479 7F7D433241 0 0 0 0 45 45 0 45

7F7D517479 486838404F 0 0 0 0 60 60 0 60

2014 TOTAL 0 0 0 0 105 105 0 105


1F48421542 1F53312736 0 0 0 0 0 0 0 0

1F48421542 7F7D376F73 0 0 0 0 0 0 0 0

1F48421542 115556461A 0 0 0 0 60 60 0 60

2015 TOTAL 0 0 0 0 60 60 0 60

79


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked



462268474B 220E3F2578 0 0 0 0 30 30 0 30

462268474B 1F4772396F 0 0 0 0 15 15 0 30

462268474B 452738076E 0 0 0 0 31 31 0 62

470378405D 220F0E6207 0 0 0 0 15 15 0 30

4A46717168 7F7D23292E 0 0 0 0 30 30 0 60

4A46717168 7F7F065834 0 0 0 0 15 15 0 30

7F7D517479 0A180E0E7E 0 0 0 0 30 30 0 60

7F7D517479 411D0B4E09 0 0 0 0 10 10 0 20

7F7D517479 7F7D7C2447 0 0 0 0 10 10 0 20

2016 TOTAL 0 0 0 0 186 186 0 342

7F7D7C2E4B 115633183A 0 0 0 0 22 22 0 44

6C00040979 7F7B024F2D 0 0 0 0 31 31 0 62

4A3E2D7E73 220E4E4E5D 0 0 0 0 16 16 0 32

4A49070E13 220E4E4E5D 0 0 0 0 13 13 0 26

4A49070E13 7F7E42795C 0 0 0 0 33 33 0 66

2017 TOTAL 0 0 0 0 115 115 0 230


80


Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Totalstocked


2020

Garrison DamNFH

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

2020TOTAL 0 0 0 0 0 0 0 0

GRAND TOTAL (Fry not included) 0 1349 1349 0 466 466 601 2687

81



Year

Class

Hatchery Female

Pit tag

Male

Pit tag


Leavenworth/St. Helena Bellevue/Sioux City Boonville Mulberry Bend /KC


7F7F06672B 7F7D3C5708 0 0 0 0 199 199 0 198 198 0 173 173 570

7F7F06672B 115631222A 0 0 0 0 118 118 0 180 180 0 133 133 431

411D262C1F 1F4A4B5973 0 0 0 0 21 21 0 20 20 0 28 28 69

411D262C1F 411D0E2C5F 0 0 0 0 555 555 0 558 558 0 557 557 1670

411D262C1F 41476A0462 0 280 280 0 579 579 0 560 560 0 321 321 1740

411D262C1F 411D0B4E09 0 0 0 0 0 0 0 0 0 0 0 0

411D262C1F 17509415139 0 0 0 0 51 51 0 52 52 0 67 67 170

220E345E09 1F4A111C6A 0 0 0 0 336 336 0 411 411 0 339 339 1086

220E345E09 1F4A27214F 0 0 0 0 250 250 0 256 256 0 256 256 762

2001 TOTAL 0 280 280 0 2109 2109 0 2235 2235 0 1874 1874 6498


116224546A 116167123A 0 500 500 399 399 520 520 1419

116224546A 1F477B3A65 0 500 500 245 245 578 578 1323

116224546A 220F107A6F 0 556 556 470 470 575 575 1601

116224546A 7F7D461025 0 382 382 327 327 396 396 1105

82


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



2002 TOTAL 0 0 0 0 1938 1938 0 1441 1441 0 2069 2069 5448


132256586A 132114552A 0 108 108 13 101 114 13 99 112 16 0 16 350

132256586A 132157621A 0 115 115 22 121 143 22 126 148 32 0 32 438

132256586A 1F47760123 0 66 66 68 71 139 68 71 139 71 0 71 415

44426F185B 1F4A363031 0 37 37 0 37 37 0 36 36 0 0 0 110

44426F185B 1F521B1E56 0 67 67 104 69 173 104 67 171 109 0 109 520

44426F185B 7F7D365422 0 220 220 45 222 267 45 221 266 46 0 46 799

44426F185B 7F7D291A07 0 48 48 149 48 197 147 45 192 147 0 147 584

7F7F054855 115669540A 0 33 33 15 40 55 20 39 59 0 0 0 147

7F7F054855 115675486A 0 66 66 27 70 97 27 70 97 22 0 22 282

7F7F054855 132313521A 0 65 65 0 39 39 0 0 0 0 0 0 104

2003 TOTAL 0 825 825 443 818 1261 446 774 1220 443 0 443 3749


114476216A 7F7D487531 286 0 286 305 11 316 286 0 286 0 6 6 894

114476216A 1F4A4B5973 69 0 69 69 25 94 69 0 69 0 31 31 263

114476216A 7F7E55466D 392 0 392 392 135 527 399 0 399 0 122 122 1440

114476216A 1F4A312640 896 0 896 220 226 446 842 0 842 0 323 323 2507

114476216A 430E452777 651 0 651 673 119 792 845 0 845 0 235 235 2523

114476216A 1F477B3A65 0 0 0 0 42 42 0 0 0 0 4 4 46

114476216A 431565767B 0 0 0 0 19 19 0 0 0 0 17 17 36

83


Year

Class

Hatchery Female

Pit tag

Male

Pit tag



114476216A 220F107A6F 0 0 0 0 24 24 0 0 0 0 10 10 34

114476216A 1F4A27214F 0 0 0 0 11 11 0 0 0 0 6 6 17

115555495A 431565767B 0 0 0 0 340 340 0 0 0 0 33 33 373

115551683A 115552116A 0 0 0 0 82 82 0 0 0 0 37 37 119

115551683A 7F7D3C555D 0 0 0 0 220 220 0 0 0 0 86 86 306

7F7F066452 114473737A 0 0 0 0 9 9 0 0 0 0 7 7 16

7F7F066452 7F7F065834 0 0 0 0 103 103 0 0 0 0 47 47 150

454910202B 1F47606357 363 0 363 0 225 225 330 0 330 0 148 148 1066

454910202B 220F0F7677 213 0 213 0 294 294 152 0 152 0 264 264 923

454910202B 115679374A 140 0 140 0 70 70 69 0 69 0 157 157 436

454B380D60 1F4A3E1445 0 0 0 0 70 70 0 0 0 0 51 51 121

454B380D60 7F7D376F73 0 0 0 0 117 117 0 0 0 0 43 43 160

454B380D60 7F7F065834 0 0 0 0 91 91 0 0 0 0 182 182 273

1F5330401E 0 0 0 0 174 174 0 0 0 0 418 418 592

MIX MIX 0 0 0 0 151 151 0 0 0 0 139 139 290

2004 TOTAL 3010 0 3010 1659 2558 4217 2992 0 2992 0 2366 2366 12585

84

Appendix 8. Females Used in Upper Basin Recovery Effort

PIT Tag PIT Tag 2 RPA1 RPA2 RPA3 RECAP Last CaptureDate

Weight(lbs)

Latest Fate Status Radio

113953385A 4/25/2006 51 SPAWNED @ GDNFH; NO OVULATION114476216A 43104B266F 0 11685 170 59 11/13/2006 39 RELEASED CODE 114115551683A 4063767709 185 415 44 30 10/24/2005 47 SPAWNED @ GPNFH IN 2006; DIED115553544A 302 2355 142 48 4/30/2013 34 RELEASED CODE 13 115555495A 26 6 4/22/2004 66 SPAWNED @ GDNFH; DIED115557165A 1073 2136 60 622 4/20/2016 45 SPAWNED @ GDNFH; 271,566 FRY STOCKED CODE 116 115557463A 4639 6065 75 196 4/22/2008 33 SPAWNED @ MCSFH; RELEASED115676635A 241 2755 122 6/23/2017 55 RELEASED CODE 95115676694A 5/5/2003 37 SPAWNED @ GDNFH; FRY DIED115679394A 5/5/2003 28 SPAWNED @ MCSFH; EGGS DIED115713555A 4/13/2000 57 SPAWNED @ GDNFH; FRY DIED CODE 62115716093A 5/16/2014 FOUND DEAD ON SHORE CODE 31 116224546A 3986 601 462 4/29/2002 60 SPAWNED @ GDNFH; RELEASED132253516A 4/21/2004 62 SPAWNED @ GDNFH; DIED132256586A 536 581 202 70 4/30/2003 SPAWNED @ MCSFH, DIED1F47715752 4/11/2000 55 SPAWNED @ GDNFH; ATRITIC CODE 251F477B7E21 5/6/2008 59 SPAWNED @ GDNFH; FRY DIED1F48421542 2722 1488 180 98 5/11/2017 50 RELEASE CODE 87 1F497F1801 11441 28997 2337 1126 4/19/2016 42 SPAWNED @ GDNFH; 61,549 FRY STOCKED CODE 91F4A301354 290 103 199 359 4/26/2010 46 RADIO IMPLANTED; RELEASED CODE 381F4A436E66 6828 4848 60 36 7/6/2011 FOUND DEAD1F4B237A79 1F497F6534 0 0 0 4/24/1997 66 SPAWNED @ GDNFH; DIED1F4B246E04 443 677 239 397 9/26/2005 53 RADIO IMPLANTED; RELEASED CODE 1211F5330401E 1F521F363A 4 5 18 6 4/29/2004 31 SPAWNED @ MCSFH; PROGENY IN RPA41F54756038 1F5420727B 0 0 0 4/30/2002 68 SPAWNED @ GDNFH, DIED1F5569653E 1F557B761B 1125 1500 76 131 5/3/2016 32 TAKEN TO GDNFH; DIDN'T OVULATE CODE 30220E345E09 0 734 140 124 4/24/2001 62 SPAWNED @ MCSFH; RADIO CODE 10220E7E3A38 0 0 0 4/18/2007 33 RADIO IMPLANTED; RELEASED CODE 155220F01755C 2204657963 12099 20408 264 456 5/3/2017 61 RADIO IMPLANTED RELEASED CODE 43220F0F6213 0 0 0 4/18/2000 45 SPAWNED @ GDNFH; DIED40636B2945 3967 4647 88 53 4/23/2007 48 SPAWNED @ GDNFH; 44;131 FRY STOCKED4064021213 0 0 191 11 10/31/2006 50 PCR & HISTOLOGY TAKEN, RELEASED40640E415B 40640E4158?0 0 0 10/24/2005 30 RELEASED

85

4310187B69 0 0 0 4/24/2002 37 SPAWNED @ GDNFH; DIED43105F0C7E 1125 1500 219 198 5/24/2010 46 SPAWNED @ GDNFH CODE 85431532681F 0 0 0 4/25/2006 48 RADIO IMPLANTED; RELEASED CODE 95 4315327C7B 827 1170 70 107 5/8/2009 30 SPAWNED @ GDNFH CODE 664443240458 668 3817 20 240 5/9/2007 51 SPAWNED @ GDNFH; 101777 FRY STOCKED4443250A24 1176 1532 138 164 5/2/2017 55 RADIO IMPLANTED; RELEASED CODE 12744635F477B 1002 1592 80 107 5/6/2013 44 RELEASED454910202B 147 4524 41 20 3/28/2014 FOUND DEAD BY ANGLER454B380D60 1005 7107 127 67 5/20/2015 33 RELEASED CODE 37 454B490528 1603 3285 194 206 4/22/2009 46 SPAWNED @ MCSFH; 7611 FRY STOCKED470378405D 477 4188 151 70 4/19/2016 40 SPAWNED @ GDNFH; 138,833 FRY STOCKED CODE 3647037F460C 3945 3780 102 64 4/24/2007 57 SPAWNED @ GDNFH; 143,497 FRY STOCKED470468383C 2612 3718 73 143 10/15/2008 54 SPAWNED @ GPNFH470A675627 14194 30919 1421 346 5/18/2016 64 SPAWNED @ GDNFH; 159,995 FRY STOCKED CODE 3547151A3D3A 8023 6264 102 97 4/25/2007 66 SPAWNED @ GDNFH; 43,533 FRY STOCKED486A080614 465 747 0 105 4/24/2012 54 SPAWNED @ GDNFH; RADIO IMPLANTED CODE 444A46717168 464 400 45 12 4/20/2016 56 SPAWNED @ GDNFH; 7,133 FRY STOCKED CODE 1224A49070E13 604 700 48 5/2/2017 33 SPAWNED @ GDNFH CODE 1247F7B016070 44426F185B 556 0 224 56 5/14/2003 51 SPAWNED @ GDNFH; DIED7F7B021573 0 478 181 68 10/6/1998 65 SPAWNED @ GPNFH; RELEASED7F7B025D51 471 3636 107 34 10/31/2006 65 SPAWNED @ GPNFH; 71,328 FRY STOCKED7F7B026102 2157 5946 155 193 4/27/2005 SPAWNED @ GDNFH; RELEASED7F7B081169 220D0A6D37 0 0 0 9/25/1997 51 SPAWNED @ GPNFH; FRY DIED7F7D373C61 0 0 0 10/7/1992 31 TAKEN TO GPNFH; DIED 7/3/947F7D43086C 0 0 0 9/11/1993 66 TAKEN TO GPNFH; DIED 6/5/947F7D517479 1494 1417 155 80 4/19/2016 34 SPAWNED @ GDNFH; 13,080 FRY STOCKED CODE 347F7D7C2E4B 612 440 22 5/23/2017 39 SPAWNED @ GDNFH CODE 397F7F054855 1841 1886 89 106 4/24/2003 SPAWNED @ MCSFH, DIED 7F7F056171 0 200 98 54 4/22/1998 65 SPAWNED @ GDNFH; DIED7F7F065E12 0 0 0 9/30/1998 65 SPAWNED @ GPNFH; DIED7F7F066452 115559532A 2109 5026 334 131 4/22/2008 26 SPAWNED @ MCSFH7F7F06672B 0 545 138 69 4/24/2001 48 SPAWNED @ MCSFH; DIED7F7FD66963 220E5B3803 5609 16787 257 283 4/18/2016 36 SPAWNED @ GDNFH; 47,593 FRY STOCKED CODE 40

86

Appendix 9. Males Used in Upper Basin Recovery Effort

PIT Tag PIT Tag 2 RPA1 RPA2 RPA3 RECAP Last CaptureDate

Weight(lbs)

Latest Fate Status Radio

113719262A 160 65 30 9/22/1998 27 SPAWNED @ GPNFH; RELEASED114473737A 6 1 4/22/2004 41 SPAWNED @ GDNFH; RADIO IMPLANTED CODE 29 115525534A 2801 6816 167 143 8/26/2014 34 RELEASED CODE 5 115529097A 567 1565 20 67 5/21/2014 29 RADIO IMPLANTED; RELEASED CODE 79115544332A 4/23/2002 55 SPAWNED @ GDNFH; DIED115552116A 5285 1242 65 31 4/21/2009 31 RELEASED CODE 24 115553761A 2157 5196 143 180 6/1/2015 44 RADIO IMPLANTED; RELEASED CODE 21115556461A 1073 2136 60 43 5/25/2017 43 RELEASED CODE 12 115626162A 6311 11518 522 192 6/18/2016 31 RADIO IMPLANTED; RELEASED CODE 18115631222A 43154A1133 6311 11518 522 62 8/28/2013 28 SPAWNED @ MCSFH; 1378 FRY STOCKED CODE 14115633183A 0 0 0 85 5/25/2017 29 SPAWNED @ GDNFH CODE 51115669294A 0 0 0 46 4/18/2016 36 RELEASED CODE 89115669540A 1135 1181 59 53 5/25/2017 29 SPAWNED @ GDNFH115675486A 1003 705 30 53 5/6/2014 30 RADIO IMPLANTED; RELEASED CODE 38115679374A 0 1493 24 7 5/11/2006 37 RELEASED115712453A 3237 9973 935 128 5/16/2017 33 RELEASED CODE 10 116165646A 96 267 78 59 9/20/2016 28 RELEASED116167123A 0 645 144 44 6/3/2009 44 RELEASED132114552A 0 798 128 41 5/7/2014 22 RELEASED CODE 76 132157621A 479 2387 74 66 4/24/2007 46 SPAWNED @ GDNFH; 101,777 FRY

STOCKED132313521A 375 500 0 48 5/1/2017 37 RELEASED CODE 1931F47606357 1F4A1E4336 125 1777 17 14 4/20/2016 33 TAKEN TO GDNFH; DIDN'T SPERMIATE CODE 187 1F4772396F 4/24/2002 53 SPAWNED @ GDNFH; DIED1F47760123 5713 908 141 67 5/19/2015 34 RELEASED CODE 82 1F477B3A65 565 2298 188 137 4/23/2015 31 RELEASED CODE 62 1F477B4E51 378 3704 19 21 5/18/2011 40 RELEASED CODE 1061F482F3F2B 946 599 19 83 9/11/2017 32 RELEASED CODE 741F4849755B 2153 6341 680 92 5/25/2010 25 RELEASED CODE 1181F49517057 9/23/1997 53 SPAWNED @ GPNFH; FERT EGGS DIED1F4A0B1A72 11441 28997 2337 204 5/22/2017 33 IMPLANTED RADIO; RELEASED CODE 71 1F4A111C6A 0 0 0 66 4/22/2008 35 RADIO IMPLANTED; RELEASED CODE 53 1F4A13453F 0 0 0 29 5/12/2017 37 SPAWNED @ GDNFH CODE 201F4A13592B 220D4E6A57 1299 3851 382 42 5/19/2016 31 RELEASED CODE 32

87

1F4A143350 375 500 96 63 6/22/2017 25 RELEASED CODE 196 1F4A206A0D 276 2216 19 6 5/7/2012 23 RELEASED CODE 221F4A24076C 1F4A4E5772 375 500 0 18 4/18/2016 20 TAKEN TO GDNFH; DIDN'T SPERMIATE CODE 49 1F4A27214F 0 366 195 100 4/22/2004 RELEASED CODE 21F4A3E1445 1F4A2F3A2E 970 2696 394 198 5/16/2017 34 RELEASED CODE 23 1F4A312640 21 1884 67 17 4/18/2007 52 RELEASED; RADIO IMPLANTED CODE 145 1F4A33194B 375 500 75 77 4/19/2016 31 RADIO IMPLANTED; RELEASED CODE 130 1F4A363031 730 813 68 45 4/25/2012 39 RADIO IMPLANTED; RELEASED CODE 55 1F4A435004 57 553 60 22 5/22/2017 33 SPAWNED @ GDNFH CODE 681F4A4A1439 1F47671023 295 314 156 276 ?/?/1997 SPAWNED @ GPNFH; RELEASED1F4A5A5A63 615 2207 0 29 4/29/2008 29 RADIO IMPLANTED; RELEASED CODE 12 1F4B225A1A 926 444 88 57 4/25/2012 34 TO GDNFH; CRYO PRODUCED HRPS IN 20131F50072169 593 1832 271 125 5/3/2017 28 RELEASED CODE 67

1F521B1E56 1F54696C38 297 440 60 7 4/21/2004 RELEASED - cryo in 2017, progeny stocked1F53312736 1F52167900 900 493 60 30 4/19/2016 40 RADIO IMPLANTED; RELEASED CODE 1322202236E31 0 0 0 4/21/2004 RELEASED CODE 442204583665 7F7F066A40 726 5605 19 31 4/18/2006 RELEASED; CRYO PROGENY STOCKED220E3F2578 220E3F7096 334 400 30 6 6/22/2016 SPAWNED @ GPNFH WITH CRYO MILT220E4E4E5D 401 566 46 17 5/22/2017 36 SPAWNED @ GDNFH CODE 52 220E5E551E 220D0E1F0B 1772 3770 0 66 4/21/2016 28 RELEASED CODE 72220E5F4928 228 1560 0 21 5/8/2007 35 SPAWNED @ GDNFH; RELEASED CODE 129220F0E6207 336 428 15 9 5/19/2016 37 SPAWNED @ GDNFH; CRYOED MILT CODE 63220F0F7677 13 1277 0 3 4/26/2017 28 RADIO IMPLANTED; RELEASED CODE 15220F107A6F 292 2173 144 260 5/2/2017 33 SPAWNED @ GDNFH CODE 192224076523 174 277 0 32 5/8/2012 36 SPAWNED @ GDNFH CODE 65 40637D481B 0 0 0 4/22/2008 64 SPAWNED @ MCSFH; DID NOT SPERMIATE430E452777 0 4873 20 14 4/29/2013 38 GENETICS TAKEN; RELEASED CODE 154310556551 7F7D4A7758 2048 3125 26 77 5/28/2014 34 RELEASED CODE 7343105C602B 2582 3461 43 90 5/8/2012 26 RADIO IMPLANTED; RELEASED CODE 75 431565767B 6244 1007 65 44 4/23/2008 48 RADIO IMPLANTED; RELEASED CODE 128435F71414F 220E5F6E26 619 886 27 40 5/25/2010 52 RELEASED CODE 154435F7C1210 387 352 0 39 5/30/2007 33 RELEASED444171072D 824 1546 0 215 5/27/2010 29 RELEASED CODE 104441774C6E 229 1895 86 36 5/23/2017 31 RELEASED CODE 81444334021A 1311 3027 46 128 5/25/2017 37 RADIO IMPLANTED RELEASED CODE 93 4443422E34 7F7D434B54 311 1532 19 17 6/26/2007 CRYO @ GDNFH; 6140 FRY STOCKED4443610736 0 0 0 5/10/2006 41 SPAWNED @ GDNFH; NO SPERM44436E2975 375 500 75 45 9/30/2014 37 RADIO IMPLANTED; RELEASE CODE 91 44440A7B73 4443332814 3576 906 26 72 5/16/2017 32 RELEASED CODE 59454B30016B 3166 443 20 3 4/19/2016 33 SPAWNED @ GDNFH; MILT CRYOED CODE 42

88

454B385D67 0 0 0 4/26/2007 33 TAKEN TO GDNFH W/ LESIONS; DIED465B6F1939 1441 2133 37 83 5/14/2014 48 RELEASED CODE 195 465D43745B 413 790 138 66 4/18/2006 SPAWNED @ MCSFH466C473C59 722 1614 0 230 5/7/2012 42 GENETICS TAKEN; RELEASED47037F3026 1480 2178 26 67 6/29/2012 35 RADIO IMPLANTED; RELEASED CODE 504704550E5B 7F7B082208 2829 7048 103 175 5/1/2017 31 RELEASED CODE 27 470A754E14 1868 3100 14 49 5/4/2017 12 RELEASED CODE 694718447879 283 436 24 34 4/29/2013 32 RADIO IMPLANTED; RELEASED CODE 179 486838404F 4310624556 665 415 60 35 5/6/2014 25 SPAWNED @GDNFH CODE 200 7F7B023253 1610 2414 165 109 5/10/2006 43 RELEASED7F7B023408 787 1120 22 47 5/16/2017 33 RELEASED CODE 90 7F7B024F2D 283 408 32 32 5/16/2017 32 SPAWNED @ GDNFH CODE 54 7F7B025248 165 266 0 41 5/29/2013 22 STOMACH PUMPED; RELEASED CODE 61 7F7B031F17 1002 4075 257 176 5/16/2017 44 RELEASED CODE 77 7F7B081579 0 0 0 4/12/2000 32 SPAWNED @ GDNFH; IRIDOVIRUS CODE 267F7B082C10 545 733 25 46 4/22/2010 67 RELEASED CODE 807F7D24754C 220E465E74 375 500 76 100 5/22/2017 44 RELEASED CODE 48 7F7D291A07 5951 1057 266 377 5/4/2017 40 CODE 45 7F7D2D723D 3107 7463 663 193 5/4/2017 37 RELEASED CODE 8 7F7D365422 41475D3C5D 2175 3637 159 131 5/23/2017 35 RELEASED CODE 787F7D372A6B 3269 2359 64 31 6/3/2009 39 RADIO IMPLANTED; RELEASED CODE 177F7D37642C 4415 9263 185 260 4/20/2016 39 RADIO IMPLANTED; RELEASED CODE 137F7D376F73 1F54714656 919 594 80 39 5/19/2015 35 SPAWNED @ GDNFH7F7D3C5708 0 247 67 17 5/13/2003 34 RELEASED CODE 1167F7D433241 223 245 45 31 5/6/2014 29 SPAWNED @ GDNFH7F7D437250 7F7D3C555D 804 1240 63 74 5/19/2016 31 435F557624 , RELEASED CODE 467F7D441774 7F7D400B19 0 159 66 17 10/15/1997 52 SPAWNED @ GPNFH; RELEASED7F7D7C2447 0 0 0 14 5/3/2017 20 RELEASED CODE 117F7E55466D 1F557B2071 3635 13646 38 78 8/21/2017 35 RELEASED CODE 867F7F054773 0 0 0 4/11/2000 50 SPAWNED @ GDNFH; IRIDOVIRUS7F7F054D0D 0 0 0 4/27/1997 41 SPAWNED @ GDNFH; EGGS DIED7F7F05635A 0 0 0 8/21/2017 23 RELEASED CODE 1317F7F056372 0 0 0 5/13/2003 28 RELEASED7F7F05660D 0 0 0 6/5/1991 42 RELEASED7F7F065834 327 931 48 26 4/19/2016 33 SPAWNED @ GDNFH; 159.995 FRY STOCKED CODE 83 7F7F06583D 151 315 129 143 9/14/1993 27 SPAWNED @ GPNFH IN 1997; RELEASED7F7F065A4D 0 0 0 4/18/2000 22 RELEASED7F7F065A4E 0 0 0 4/22/2003 TAKEN TO MCSFH, DIED7F7F066471 115667264A 4648 10472 596 110 5/6/2017 33 RADIO IMPLANTED; RELEASED CODE 577F7F06697C 1846 2750 62 136 6/1/2017 39 RELEASED CODE 60

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Appendix 10. RPA2 Unknown Sex Wild Broodstock

PIT Tag PIT Tag 2 RPA1 RPA2 RPA3 Last CaptureDate

Weight(lbs)

RECAP Latest Fate Status Radio

115529091A 0 0 0 11/1/2006 33 RELEASED115554596A 0 0 0 7/12/2005 58 RELEASED115555446A 0 0 0 7/14/2005 25 RELEASED115676690A 0 0 0 4/13/2000 12 RELEASED; POSSIBLE HYBRID116175735A 0 0 0 5/7/2018 TAKEN TO GDNFH116216195A 0 0 0 5/13/2003 RELEASED1F47671023 0 0 0 10/21/1997 29 RELEASED1F49516C5B 0 0 0 4/20/2016 26 TAKEN TO GDNFH; NO RESULTS CODE 1501F4A504C7B 0 0 0 9/27/1994 22 RELEASED1F55776D28 1F520F7C04 0 0 0 8/24/1995 37 RELEASED22045E534D 0 0 0 5/8/2003 0 RELEASED220C7D0429 2204523542 0 0 0 4/28/2004 31 RELEASED220E587576 0 0 0 4/25/2001 12 RELEASED220E681454 0 0 0 4/28/1998 48 RELEASED220E770F3F 0 0 0 5/13/2003 24 RELEASED4443706E51 0 0 0 10/6/2005 40 RELEASED444374235B 0 0 0 9/13/2006 33 RELEASED44440F4A35 0 0 0 9/20/2005 18 RELEASED487D471D18 0 0 0 8/21/2017 16 1997 YEAR CLASS CODE 1176C00112905 0 0 0 5/25/2017 32 RADIO IMPLANTED; RELEASED CODE 94 7F7B015B78 0 0 0 11/15/1994 28 RELEASED7F7B020D71 1F541E4A25 0 0 0 8/25/1995 44 RELEASED7F7B024922 0 0 0 5/31/1995 36 RELEASED7F7B035C47 0 0 0 4/22/1993 33 RELEASED7F7B035D1B 0 0 0 9/28/1993 45 RELEASED7F7B08162E 1F54715E3E 0 0 0 9/29/1995 32 RELEASED7F7B082C14 0 0 0 9/7/1994 28 RELEASED7F7D26595E 0 0 0 9/21/1994 76 RELEASED7F7D266242 0 0 0 4/18/2000 29 RELEASED7F7D27307F 0 0 0 4/25/1993 9 RELEASED7F7D292179 0 0 0 9/27/1994 26 RELEASED7F7D337721 0 0 0 4/27/1993 28 RELEASED

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7F7D352A52 0 0 0 4/26/1997 39 RELEASED7F7D35317F 0 0 0 11/15/1994 RELEASED7F7D364B62 0 0 0 9/25/2002 27 RELEASED7F7D365140 0 0 0 9/25/1994 40 RELEASED7F7D37765C 0 0 0 9/24/1994 46 RELEASED7F7D380720 0 0 0 9/25/1994 49 RELEASED7F7D381776 0 0 0 9/11/1993 52 RELEASED7F7D3E6D23 0 0 0 10/22/1992 27 RELEASED7F7D433F0C 0 0 0 4/13/2000 39 RELEASED7F7D43515A 0 0 0 9/10/1993 25 RELEASED7F7D435261 0 0 0 9/25/1994 50 RELEASED7F7D441A7C 0 0 0 10/27/1992 30 RELEASED7F7D44647E 0 0 0 9/29/1993 26 RELEASED7F7D4B0A42 0 0 0 9/9/2013 10 RADIO IMPLANTED; RELEASED7F7D7B1607 0 0 0 5/30/1997 RELEASED7F7E427F69 1F555C367A 0 0 0 10/12/1995 60 RELEASED7F7E690405 0 0 0 5/21/1993 RELEASED7F7E6B3364 0 0 0 10/6/1992 32 RELEASED7F7E6B3711 0 0 0 9/9/1993 28 TAKEN TO GPNFH; DIED 7/12/947F7F054864 0 0 0 9/16/1993 24 RELEASED7F7F054B4E 0 0 0 9/27/1994 28 RELEASED7F7F055428 1F545E002F 0 0 0 3/19/1996 51 RELEASED7F7F055B56 0 0 0 9/22/1994 44 RELEASED7F7F055C21 0 0 0 9/30/1992 43 RELEASED7F7F056360 0 0 0 9/30/1992 43 RELEASED7F7F056A0A 0 0 0 9/22/1994 32 RELEASED7F7F064F27 0 0 0 6/8/1994 18 RELEASED7F7F065014 0 0 0 10/19/1992 35 RELEASED7F7F06511C 0 0 0 10/5/1998 31 RELEASED7F7F065841 0 0 0 3/21/1989 29 RELEASED7F7F065A3D 0 0 0 4/11/2000 55 RELEASED7F7F065C30 0 0 0 3/10/1989 33 RELEASED7F7F065F14 0 0 0 9/24/1994 32 RELEASED7F7F066128 0 0 0 9/26/1994 47 RELEASED7F7F06617F 0 0 0 5/15/1994 RELEASED7F7F06627E 0 0 0 6/17/1992 28 RELEASED7F7F066502 0 0 0 9/28/1994 28 RELEASED7F7F066613 0 0 0 9/14/1990 39 RELEASED

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7F7F06685C 0 0 0 8/4/1999 40 RELEASED7F7F06685E 0 0 0 9/23/1991 50 RELEASED7F7F066A0E 0 0 0 9/13/1990 33 RELEASED7F7F066A45 0 0 0 9/26/1994 34 RELEASED7F7F066A66 0 0 0 5/31/1991 32 RELEASEDLAMTAG 1111 7/18/1991 25 RELEASEDYE DANGLER TAG 10/6/1992 28 RELEASEDORANGE #7-0049 5/21/1994 RELEASEDAVID TAG *009*28 6/16/1994 25 RELEASEDAVID TAG *010*11 6/16/1994 22 RELEASEDDANGLER 10025 PINK 8/11/1994 RELEASEDDANGLER 7-2008 RED 8/11/1994 RELEASEDDANGLER 10025 PINK 8/11/1994 RELEASEDRED DANGLER # 7-2 8/12/1994 RELEASEDCINCH TAG# 7-01174 5/28/2000 29 RELEASED

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Appendix 11. Garrison Dam NFH Hatchery Capacity

Tank Capacity at a Density of 0.5 Pounds per Square Foot

TankSize(ft)

# SquareFt (ea)

TotalSqft

Capacityof 2"fish/tank






892.86 111.61 33.07 13.95 9.80 7.14

4 Circular 10 12.6 126 5625 703 208 88 62 45

5 Circular 38 19.6 744.8 8750 1094 324 137 96 70

6 Circular 0 28.2 0 12589 1574 466 197 138 101

8 Circular 7 50.2 351.4 22411 2801 830 350 246 179

20 Circular 2 314 628 140179 17522 5192 2190 1538 1121

3 X 29 Tanks 8 87 696 38839 4855 1438 607 426 311

Total 65 2546 1136696 142087 42100 17761 12474 9094

Normal HatcheryCapacity

55 545625 68203 20208 8525 5988 4365

Overwinter Tank Capacity at a Density of 0.70 Pounds per Square Foot

TankSize(ft)

# SquareFt (ea)

TotalSqft







7.14 5.37 4.13 3.25 2.60 2.12

4 Circular 10 12.6 126 63 47 36 29 23 19

5 Circular 38 19.6 744.8 98 74 57 45 36 29

6 Circular 0 28.2 0 141 106 82 64 51 42

8 Circular 7 50.2 351.4 251 189 145 114 91 74

20 Circular 2 314 628 1570 1180 909 715 572 465

3 X 29 Tanks 8 87 696 435 327 252 198 159 129

Total 65 2546 12731 9565 7367 5795 4640 3772

Normal HatcheryCapacity

55 6111 4591 3536 2782 2227 1811

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Appendix 13. Fish Health Inspection Report

94

Appendix 14. RPA1 Variance Effective Population

The median number for males in RPA1 is 352 and for females 560.

95


Median number for males in RPA2 is 661 and for females 734.

96


The median number for males in RPA3 is 63 and for females 102.

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Table 24. Feed Study

2019 Feed StudyFemale Male Diet Tank Initial

NumberNumberon July

31

%survival

tankweight (g)

g/fish

4A49070E13 1F4A3E1445 Portman's 9 300 20 7% 14 0.704A49070E13 1F4A3E1445 Portman's 20 300 14 5% 4 0.294A49070E13 1F4A3E1445 Portman's 26 300 14 5% 3 0.214A49070E13 1F4A3E1445 Otohime 22 300 0 0%4A49070E13 1F4A3E1445 Otohime 15 300 27 9% 19 0.704A49070E13 1F4A3E1445 Otohime 14 300 17 6% 12 0.714A49070E13 1F4A3E1445 Razorback 4 300 7 2% 1 0.144A49070E13 1F4A3E1445 Razorback 28 300 0 0%4A49070E13 1F4A3E1445 Razorback 21 300 10 3% 6 0.604315327C7B 1F4A3E1445 Portman's 17 300 79 26% 41 0.524315327C7B 1F4A3E1445 Portman's 6 300 73 24% 37 0.514315327C7B 1F4A3E1445 Portman's 12 300 48 16% 23 0.484315327C7B 1F4A3E1445 Otohime 23 300 19 6% 10 0.534315327C7B 1F4A3E1445 Otohime 1 300 51 17% 24 0.474315327C7B 1F4A3E1445 Otohime 7 300 52 17% 27 0.524315327C7B 1F4A3E1445 Razorback 16 300 72 24% 19 0.264315327C7B 1F4A3E1445 Razorback 18 300 12 4% 3 0.254315327C7B 1F4A3E1445 Razorback 8 300 43 14% 20 0.471F48421542 1F4A3E1445 Portman's 25 300 104 35% 70 0.671F48421542 1F4A3E1445 Portman's 13 300 77 26% 42 0.551F48421542 1F4A3E1445 Portman's 3 300 99 33% 60 0.611F48421542 1F4A3E1445 Otohime 10 300 106 35% 66 0.621F48421542 1F4A3E1445 Otohime 24 300 20 7% 11 0.551F48421542 1F4A3E1445 Otohime 5 300 84 28% 41 0.491F48421542 1F4A3E1445 Razorback 11 300 48 16% 21 0.441F48421542 1F4A3E1445 Razorback 19 300 61 20% 27 0.441F48421542 1F4A3E1445 Razorback 2 300 58 19% 26 0.451F48421542 1F4A3E1445 Artemia 27 300 21 7% 3 0.141F48421542 1F4A3E1445 GP Advanced 29 300 0 0%

1F48421542 1F4A3E1445 Gold Pearl Re 30 300 116 39% 82 0.71

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Chart 7. Feed Study

Table 25. Feed Study Evaluation

Feed Study EvaluationFemale 7C7B 7C7B 7C7B 1542 1542 1542 0E13 0E13 0E13

Male 1445 1445 1445 1445 1445 1445 1445 1445 1445Diet Otohime Razerback Portman's Otohime Razerback Portman's Otohime Razerback Portman's

Total Mortality 778 773 700 690 733 620 856 883 852

% Mortality 86% 86% 78% 77% 81% 69% 95% 98% 95%

Family Mortality 83% 76% 96%

Fish on Feed 122 127 200 210 167 280 44 17 48

Fish Weight (g) 61 42 101 118 74 172 31 7 21

g/fish 0.50 0.33 0.51 0.56 0.44 0.61 0.70 0.41 0.44

Diet Mortality Otohime 86% Razerback 88% Portman's 80%

g/fish Otohime 0.59 Razerback 0.40 Portman's 0.52

Diet Survival g/fish

Otohime 14% 0.59

Razerback 12% 0.40Portman's 20% 0.52

GP Adv 0% 0.00Artemia 7% 0.14

Gold Pearl 39% 0.71

Female Survival g/fish

7C7B 17% 0.451542 24% 0.540E13 4% 0.52

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List of Tables

Chart 1. Larval Mortality by Family Lot in 2004 Feeding Biodiet Diets......................................................2

Chart 2. Larval Mortality by Family Lot in 2017 Feeding Otohime Diet.......................................................3

Table 1. Pallid Broodstock Captures.............................................................................................................5

Table 2. RPA2 Adults Captured ...................................................................................................................6

Table 3. Broodstock Data..............................................................................................................................9

Table 4. Milt Collections.............................................................................................................................10

Table 5. Stocking Targets by RPA (Median Numbers)..............................................................................10

Table 6. Proposed Stocking Targets......................................................................................................11-15

Table 7. Female # 4443250A24 - Spawning Results.................................................................................16

Table 8. Female # 4A49070E13 - Spawning Results................................................................................17

Table 9. Female # 4315327C7B - Spawning Results...............................................................................18

Table 10. Female # 1F48421542 - Spawning Results...............................................................................19

Table 11. Female # 7F7D7C2E4B - Spawning Results............................................................................20

Table 12. Gavins Point NFH Captive Broodstock Larval Releases and Survival ......................................21

Table 13. Egg Hatch and Fry Survival........................................................................................................22

Table 14. Survival Summary.......................................................................................................................23

Table 15. Mortality Records to 31 Days Post Hatch..................................................................................24

Chart 3. Daily Mortality - Captive Brood Progeny to 31 DPH....................................................................25

Table 16. Initial Growth.........................................................................................................................26-27

Table 17. Feed and Food Costs....................................................................................................................28

Chart 4. Brood Temperatures North Tank.................................................................................................29

Chart 5. Brood Temperatures South Tank.................................................................................................30

Chart 6. Production Temperatures............................................................................................................31

Table 18. Proposed Spawn Strategy Using Relatedness Analysis..............................................................32

Table 19. Oocyte Maturation Assay - Female # 4A49070E13 ...................................................................33

Table 20. Oocyte Maturation Assay - Female # 7F7D7C2E4B...................................................................34

Table 21. Oocyte Maturation Assay - Female # 1F48421542......................................................................35

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Table 22. Oocyte Maturation Assay - Female # 4443250A24....................................................................36

Table 23. Oocyte Maturation Assay - Female # 4315327C7B.....................................................................37

Appendix 1. Milt Repository at Garrison Dam NFH.............................................................................38-39

Appendix 2. Cryopreserved Milt Repository - Upper Basin Wild..............................................................40

Appendix 3. RPA2 Missouri River Stockings from GDNFH................................................................41-54

Appendix 4. RPA2 Yellowstone River Stockings from GDNFH..........................................................55-65

Appendix 5. RPA1 Stockings from GDNFH.........................................................................................66-73



Appendix 8. Females Used in Conservation Propagation in the Upper Basin ......................................85-86

Appendix 9. RPA2 Males.......................................................................................................................87-89

Appendix 10. RPA2 Pallids of Unknown Sex........................................................................................90-92

Appendix 11. Hatchery Capacity..................................................................................................................93

Appendix 13. Fish Health Inspection Report...............................................................................................94

Appendix 14. RPA1 Variance Effective Population...................................................................................95



Table 24. Feed Study Data...........................................................................................................................98

Chart 7. Feed Study......................................................................................................................................99

Table 25. Feed Study Evaluation.................................................................................................................99

Appendix 17. Decision Letter Not to Stock F2 Progeny...................................................................100-101

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PALLID STURGEON PROPAGATION 2019 Garrison Dam NFH

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