p FLUORESCENCE MICROSCOPY
Transcript of p FLUORESCENCE MICROSCOPY
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FLUORESCENCE MICROSCOPY
ELSEVIER
SUPER-RESOLUTION· AND OTHER NOVEL TECHNIQUES
Edited by
ANDA CoRNEA AND P. MrcHAEL CoNN
AMSTERDAM • BOSTON • HEIDELBERG • LONDON
NEW YORK • OXFORD • PARIS • SAN DIEGO
SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO
Academic Press is an Imprint of Elsevier
Contents
Preface vii List of Contributors lX
~ · ~~, . -.. .. . ' ..... :...,. ·: ... \. l. Evanescent Excitation and Emission ·. : .. ~ · :·.
DANIEL AXELROD
Introduction Evanescence in General 2 Evanescence in Excitation: TIR 2 Evanescence in Emission: The Fluorophore Near Field 7
Evanescence from a Point Source: NSOM 13
2. Adaptive Optics for Fluorescence Microscopy MARTIN J. BOOTH AND BRIAN R. PATTON
Introduction 15 Aberrations in Microscopy 15
Principles of Adaptive Optics 20
Adaptive Aberration Correction in Fluorescence Microscopes 22 Wavefront Sensing 26
Schemes for Modal Sensorless Adaptive Optics 28 Conclusion 32
3. Rapid Measurements of Orientation and Rotation in Ex Vivo Muscle Made Possible by Studying
Small Number of Cross-Bridges
Introduction 35
Kinetics 38
JULIAN BOREJDO
Distribution of Cross-Bridge Orientations 43 Conclusions 46
4. High Spatiotemporal Bioimaging Techniques to Study the Plasma Membrane N anoscale
Organization MARJOLEIN B.M. MEDDENS, SANDRA DE KEI]ZER, AND
ALESSANDRA CAMBI
Introduction 49
Spatial Resolution 50
Temporal Resolution 54 Conclusions and Outlook 59
v
S. High-Resolution 30 Imaging of Intact Transparent Organs by 3DISCO
ALl ERTORK
Introduction 65
Current Technologies for Imaging the Intact Brain 65
3DISCO 73
6. Using RNA Mimics of GFP to Image RNA Dynamics in Mammalian Cells RITA L. STRACK AND SAM IE R. jAFFREY
Introduction 83
Studying Trinucleotide-Repeat-Containing RNA 85 Optimizing Imaging of CGG Repeat-Containing RNA 86
Time-Lapse Imaging of CGG-Repeat•Containing RNAs 86
Monitoring CGG Repeat Foci During Cell Division 87
Examining the Stability of CGG6o RNA 88
Imaging the Effects of Small-Molecule Drugs on CGG Foci 88
Imaging Other RNAs Using Spinach2 89
Troubleshooting Imaging with Spinach2-Tagged Constructs 89 Imaging Rare RNAs 90
Future Directions 90
7. Surface Enhanced Raman Scattering (SERS) Image Cytometry for High-Content Screening
ER LIU AND JOHN P. NOLAN
High-Content Screening: Status and Needs 93
Labels and Probes for HCS: Fluorescence and SERS 93
Spectral Imaging for Fluorescence and SERS 95 SERS Imaging in Cell-Based Assays 101
Status and Prospects 105 Appendix 107
8. Light Sheet Fluorescence Microscopy Applications for Multicellular Systems
JULIEN COLOMBELU AND CORINNE LORENZO
Introduction 109
LSFM Implementations: A Universal Concept with Many Acronyms 111
LSFM at the Macroscopic Scale 111 3D Cancer Imaging Model: MCTS 113
Calcium Imaging, Fast Volumetric Calcium Imaging 116
Conclusions 118
Vl CONTENTS
9. Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography
Analysis MELANIE BOKSTAD AND OHAD MEDAL!A
Introduction 121
Electron Microscopy in Cellular and Structural Biology 121
Sample Preparation for Electron Microscopy 122
Cryo-Electron Tomography 123 Correlative Light and Electron Microscopy (CLEM) 125
Correlated Light and Electron Microscopy Direct Observation • in Cryogenic Temperatures 127
CLEM Prospects 130
10. Cellular and Molecular Applications of Super-resolution Microscopy
EUGENIO F. FORNASIERO AND FELIPE OPAZO
Introduction 133
uper-res lution Microscopy Techniques 134 Troublcsh ting Sample Preparation 137
Thick, Li ving, Multicolor JD amplcs: Friends and Foes of uper-resolution Microscopy l40
Biological Applications 142 Conclusions 150
11. Use of Engineered Nanoparticle-Based Fluorescence Methods for Live-Cell Phenomena
CHAN-G! PACK, MIN-KYO JUNG, Ml-ROUNG SONG, )UN-SUNG KIM, SUNG-SJK HAN, AND YASUSH! SAKO
Introduction 153
Materials and Methods 154
Results and Discussion 157
Conclusions 165 Perspectives 166
12. High-Resolution Estimation of Multiple Cell Populations in Tissue Using Confocal Stereology
DANIEL A. PETERSON
Introduction 171 Design-Based Stereology as a Quantitative Tool 172
Sampling Requirements to Achieve a Population Estimate with High Resolution 176
Practical Requirements for Performing Stereology 179
Separation of Image Acquisition and Probe Analysis 180
Implementation of Systematic Random Sampling on a Confocal Microscope 181
Virtual Tissue with Post-Acquire Sampling 183
Conclusions 183
Conflict of Interest Disclosure 183
13. Multiphoton Microscopy Applications in Biology
MIKO YAMADA, LYNLEE L. LIN, AND TARL W. PROW
Introduction 185 Multiphoton Microscopy Applications 188
14. Super-resolution Microscopy: A Comparison of Commercially Available Options
jENNIFER A. THORLEY, JEREMY PIKE, AND JOSHUA Z. RAPPOPORT
Introduction 199
Technical Considerations 201 Single-Molecule Localization Microscopy 203
Structured Illumination Microscopy 206
Stimulated Emission Depletion Microscopy 208
Summary 210
Future Directions and Emerging Techniques 210
15. Structured Illumination Microscopy LIN SHAO AND E. HESPER REGO
Introduction 213
Theory 213
Materials and Methods 216
Results 223
Discussion 224
16. The Role of Image Analysis Algorithms in Super-resolution Localization Microscopy
ALEX SMALL AND SHANE STAHLHEBER
Introduction 22 7 Localization Algorithms and the Problem of Noise 228
Guidelines for Selecting a Localization Algorithm 229 Dipoles and 3D Localization 230 Rejection Algorithms for Multi-Molecule Images 231
Rejection Issues in 3D 239
Speeding Up Performance with Multi-Molecule Analysis 239
Conclusions 240
Index 243