P ATTERNS OF D IVERSIFICATION OF E CONOMICALLY -I MPORTANT T REE G ENUS T ABEBUIA Nirzka M....
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PATTERNS OF DIVERSIFICATION OF ECONOMICALLY-IMPORTANT TREE GENUS TABEBUIA Nirzka M. Martínez Rivera PhD Student Biology Graduate Program UPR, Río Piedras Campus Eugenio Santiago-Valentín, PhD Associate Professor Department of Biology UPR, Río Piedras Campus
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Transcript of P ATTERNS OF D IVERSIFICATION OF E CONOMICALLY -I MPORTANT T REE G ENUS T ABEBUIA Nirzka M....
PATTERNS OF DIVERSIFICATION OF ECONOMICALLY-IMPORTANT TREE GENUS TABEBUIA
Nirzka M. Martínez Rivera
PhD Student
Biology Graduate Program
UPR, Río Piedras Campus
Eugenio Santiago-Valentín, PhD
Associate Professor
Department of Biology
UPR, Río Piedras Campus
INTRODUCTION: BIODIVERSITY HOTSPOTS
(Myers et al., 2000)
INTRODUCTION: CARIBBEAN BIODIVERSITY HOTSPOT
(2009 Google - Map Data)
60% vascular plants endemic (Santiago-Valentín and Olmstead, 2003) - 6,067 endemic species - 108 endemic genera
INTRODUCTION: GENUS TABEBUIA
OBJECTIVES
Test the monophyly of the group, and evaluate evolutionary relationships between the species.
Assess patterns of morphological evolution, and adaptive-ecological diversification among species.
Evaluate geographical relationships within the group.
METHODS
Taxonomic sampling. Caribbean and Continental Tabebuia
Field Samples Outgroups (various Tribes, especially Tecomeae)
Available Genbank sequences Field Samples
DNA extraction, amplification, and
sequencing. Chloroplast and nuclear ITS genomes
Phylogenetic analysis Maximum Parsimony Maximum Likelihood Bayesian
TAXONOMIC SAMPLING Puerto Rico (80% taxa collected)
Quebradillas, Isabela, Guánica, Carite, Yunque, Maricao, Cabo Rojo
Hispaniola (~50-60% taxa collected) Southern region
Sierra Bahoruco/Parque Nac. Jaragua/Isla Beata/Sierra de Martín García Central region
Bonao/Jarabacoa, Guaiguí, Manaclita Northern region
Puerto Plata, Dajabón, Península Samaná, Loma Quita Espuela
Cuba (8% taxa collected) Western and Central region (R. Olmstead & E. Santiago, 1996)
Cultivated material Fairchild Tropical Botanical Garden/Montgomery Research Center
(Miami) Bahamas, Jamaica, Continental (~10)
PRELIMINARY RESULTS
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Consensus from 123 most parsimonious trees
Branches with bootstrap support greater than 50% are shown above or below the branches. Branches with less than 50% are collapsed in a polytomy.
Consistency index (CI): 0.538278Retention index (RI): 0.725462
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PRELIMINARY RESULTS: GENUS TABEBUIA IS NON-MONOPHYLETIC
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Objective:
Test the monophyly of the group, and evaluate evolutionary relationships between the species.
PRELIMINARY RESULTS: CUBA-BAHAMAS/HISPANIOLA RELATIONSHIP
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T. acrophylla (Urban) Britton
Objective:
Evaluate geographical relationships within the group.
PRELIMINARY RESULTS: PUERTO RICO AND EASTERN CARIBBEAN
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T. schumanniana Urban
T. heterophylla (DC.) Britton
PRELIMINARY RESULTS:CONTINENTAL/CARIBBEAN RELATIONSHIP
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78100
T. rosea (Berteloni) DC.
T. rosea (Berteloni) DC.
FUTURE DIRECTIONS• To continue the taxonomic sampling of
the genus in additional geographical regions.
• Incorporate other nuclear and chloroplast regions to add resolution to elucidate the evolutionary relationships.
• Carry out ecological and morphological assessments of the taxa.
T. myrtifoliaScanning electron micrograph (SEM)
SPM THRUST AREA MEEG THRUST AREA
Phylogeography and Conservation Genetics of Tabebuia heterophylla (DC.) Britton
(a) Location of Tabebuia heterophylla population samples;
(b) Chloroplast DNA gene tree. Lines represent single nucleotide changes between sequences.
ACKNOWLEDGEMENTS
Center for Applied Tropical Ecology and Conservation (CATEC) - NSF - CREST Grant
Botanical Garden Herbarium, Univ. of Puerto Rico
Marcos Caraballo Adrian Valls Elson Viruet Milka Miranda Omara Ortiz Doralis Villanueva
Dr. Jason Rauscher Lab,Univ. of Puerto Rico
Dr. Jason Rauscher Yadira Ortiz Victor Santiago Angel Rivera
Dr. Tomas Hrbek Lab,Univ. of Puerto Rico
Sequencing and Genotyping Facility, Univ. of Puerto Rico
Dania Rodríguez Karla Maldonado
Universidad Autónoma de Santo Domingo. República Dominicana
Dr. Jackeline Salazar
Jardin Botánico Nacional de República Dominicana
Lic. Milcíades Mejía Lic. Ricardo García Francisco Jiménez Brígido Peguero
Fairchild Tropical Botanical Garden/Florida International University
Dr. Michael Maunder, Former Director FTBG Dr. Javier Francisco Ortega
Montgomery Botanical Center Dr. Patrick Griffith
Dr. Richard Olmstead Lab,Univ. of Washington - Seattle
New York Botanical Garden
