Oriental Aquamarine Biotech India Oriental Aquamarine Biotech India (P) LimitedCoimbatore

Nitrifying Bioreactor Technology for the Establishment of Biosecurethe Establishment of Biosecure

Recirculating Aquaculture Systems

Aquaculture

Conventional SystemsAquaculture Tanks Ocean/Waterbody

Ammonia & Nitrites

Excess FeedFecal Matter

Conventional systems – problemsNeeds large quantities of water

Conventional systems -problem

Reduced survival rate

Conventional systems-problemsCauses water pollution

Oriental Aquamarine Biotech IndiaPrivate Ltd - Coimbatore

• Technology developed by National Centre forAquatic Animal Health, Cochin University ofScience and Technology, Kerala, India

• Technology commercialized by OrientalAquamarine Biotech India Private Limited,Coimbatore, Tamil Nadu

The Solution – Bacterial ConsortiumA consortium of nitrifying bacteria have been identified which break down the ammonia and nitrites

Consortium grows as biofilm on beads packed and remove Nitrites & Ammonia inside a and remove Nitrites & Ammonia inside a bioreactor

The solution-Nitrifying Bioreactor

• PBBR • SBSBR

Nitrifying Bioreactor

Stringed Bed Suspended Bio Reactor (SBSBR)-500 liters/day

Packed Bed Bio Reactor (PBBR)Packed Bed Bio Reactor (PBBR)-60,000 liters/day

NH4+ < 0.1 ppm

NO2- < 0.5 ppm

NO3 10 – 100 ppm

Vibrio < 2 * 102 / ml

Water quality parameters in the larval rearing system and treated water stabilized as under :

Vibrio < 2 * 102 / ml

Luminescent Vibrio Nil

pH 7.5 – 8.5

No water exchange

Reef Quality Water.

PROGRESS OF NITRIFICATION INPBBR INTEGRATED PENAEUS MONODON

MATURATION SYSTEM

5

6

7

8

Con

cent

ratio

n (m

g/L)

0

1

2

3

4

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

Con

cent

ratio

n (m

g/L)

TAN NO2-N NO3-N

NITRIFICATION EFFICIENCY

0.4

0.5

0.6

Con

cent

ratio

n (m

g/L)

0.3

0.4

0.5

0.6

0.7

Concentration (mg/L)

0

0.1

0.2

0.3

Con

cent

ratio

n (m

g/L)

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

TAN-in TAN-out

0

0.1

0.2

0.3

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Days

Concentration (mg/L)

Ammonia Input Ammonia Output

NITRIFICATION EFFICIENCY

0.4

0.5

0.6

Con

cent

ratio

n (m

g/L)

0

0.1

0.2

0.3

Con

cent

ratio

n (m

g/L)

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

NO2-N-in NO2-N-0ut

2

Substrate reduction at varied concentration

2

VOLUMETRIC TAN REMOVAL RATES BY THE REACTOR WITH FLOW RATE

80

100

120

140

TAN

rem

oval

(g/

m3/

day)

0

20

40

60

250 750 1500 2500

Flow rates (L/hr)

TAN

rem

oval

(g/

m3/

day)

TAN removal Rates

TAN, NO2-N and NO3-N experimental systems integrated with SBSBR rearingPenaeus mondon

5

6

7

8

9

10

Con

cent

ratio

n (m

g/L)

0

1

2

3

4

5

0 5 10 15 20 25 30

Days

Con

cent

ratio

n (m

g/L)

TAN NO2-N NO3-N

Stringed Bed Suspended Bioreactor Deployed in a Larval rearing system at Queens Hatchery, Kodungalloore

TNFDC, Aliyar (0-PPT Trials)Fish: Koicarp (10grms)

150

200

250

300

Fish QTY

0

50

100

150

1 5 10 15 20

Days

Fish QTY

Golden fish farm ,Calicut (0-PPT Trials)Fish: Angel (Size:1cm to 3cm)

700

900

1100

1300

Fish QTY

-100

100

300

500

1 5 10 15 20

Days

Fish QTY

CMFRI -Kochi

4

5

6

7

CMFRI PERFORMANCE CHART

-2

-1

0

1

2

3

0 5 10 15 20 25 30

ppm con

cent

ration

No of days

NH3

NO2

NO3

•• Patent in IndiaPatent in India :

• Patent Application # 828/DEL/2000

•• International PatentInternational Patent :

• International patent classification: C02F 3/13.

• International application No. PCT/IN00/00097

• Technology is also patented in South East Asian Countries with a well-developed aquaculture industry such as

Thailand

Japan

Philippines

Indonesia and

South Korea.

Publication

APPLICATION

• Ornamental fish FRESH WATER MARINE WATER

• Edible fish• Edible fishSEA BASSLOBSTERSHIRMPPRAWNCRABCOBIA etc

Nitrifying Bioreactor - Advantages

• Improved quality of fish produced (Organic)• Increase in fish survival rate• Increase in rate of growth and the size of fish• Better resistance to diseases• Reduction in production costs• Improvement in surrounding environment

Nitrifying Bioreactor - Technology

• Patented technology• First of its kind in the world• Maintains reef-like conditions in aquaculture tanks• Can be used for any level of salinity

¡ Fresh Water¡ Fresh Water¡ Salt Water¡ Brackish Water

• Can be used to farm a variety of marine animals¡ Shrimp¡ Ornamental Fish¡ Crabs/Lobsters

BENEFITS

Maturation(Developing ovary in the animals reared in the recirculating system)

Brood-stock Maintenance and Rearing

Increased stocking & survival rateIncreased stocking & survival rate

RECIRCULATING AQUACULTURE SYSTEM

qLocation flexible – transport costs minimized

qFewer tanks – less capital outlay

qTemp and other parameters controlled

qPlanned production schedules, control supplies offingerlings to target seasonal market demand bycage farmers Lower mortality, improved FCRs

qRAS allow diversification of the farmed species basefor the market substituting seafood imports andopening new markets in live fish sales

Mohan KandaswamyDirector

Oriental Aquamarine Biotech India P LtdU-7 Kovaipudur, Coimbatore - 641042

Mobile : 91 94429 51725E-mail: mohan@nitrifying-bioreactor.com