Organelles Part 1-Making Proteins
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Transcript of Organelles Part 1-Making Proteins
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2007-2008 AP Biology
Tour of the Cell 1
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AP Biology
Prokaryotebacteria cellsProkaryote
bacteria cellsTypes of cells
Eukaryoteanimal cellsEukaryote
animal cells
- no organelles
- organelles
Eukaryoteplant cellsEukaryoteplant cells
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AP Biology
Why organelles? Specialized structures
specialized functions cilia or flagella for locomotion
Containers partition cell into compartments create different local environments
separate pH, or concentration of materials distinct & incompatible functions
lysosome & its digestive enzymes
Membranes as sites for chemical reactions unique combinations of lipids & proteins embedded enzymes & reaction centers
chloroplasts & mitochondria
mitochondria
chloroplast
Golgi
ER
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AP Biology
Cells gotta work to live! What jobs do cells have to do?
make proteins proteins control every
cell function make energy
for daily life for growth
make more cells growth repair renewal
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2007-2008AP Biology
Building Proteins
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AP Biology
Proteins do all the work!
cellscells
DNADNA
proteinsproteins
organismorganismRepeat after me…
Proteins do all the work!
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AP Biology
Cells functions Building proteins
read DNA instructions build proteins process proteins
folding modifying
removing amino acids adding other molecules
e.g, making glycoproteinsfor cell membrane
address & transport proteins
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AP Biology
Building Proteins Organelles involved
nucleus ribosomes endoplasmic reticulum
(ER) Golgi apparatus vesicles
nucleus ribosome ERGolgi
apparatusvesicles
The Protein Assembly Line
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AP Biology
nuclearpores
nuclearpore
nuclear envelopenucleolus
histone protein
chromosome
DNA
Function protects DNA
Structure nuclear envelope
double membrane membrane fused in spots to create pores
allows large macromolecules to pass through
Nucleus
What kind of molecules need to
pass through?
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AP Biology
DNA
NucleusmRNA
nuclearmembrane
smallribosomal
subunit
largeribosomal
subunit
cytoplasm
mRNA
nuclear pore
production of mRNA from DNA in nucleusproduction of mRNA from DNA in nucleus
mRNA travels from nucleus to ribosome in cytoplasm through nuclear pore
mRNA travels from nucleus to ribosome in cytoplasm through nuclear pore
1
2
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AP Biology
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AP Biology
Nucleolus Function
ribosome production build ribosome subunits from rRNA & proteins exit through nuclear pores to cytoplasm &
combine to form functional ribosomes
smallsubunit
large subunit
ribosome
rRNA &proteins
nucleolus
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AP Biology
smallsubunit
largesubunitRibosomes
Function protein production
Structure rRNA & protein 2 subunits combine 0.08m
RibosomesRough
ER
SmoothER
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AP Biology membrane proteins
Types of Ribosomes Free ribosomes
suspended in cytosol synthesize proteins that
function in cytosol
Bound ribosomes attached to endoplasmic
reticulum synthesize proteins
for export or for membranes
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AP Biology
Endoplasmic Reticulum Function
processes proteins manufactures membranes synthesis & hydrolysis of many compounds
Structure membrane connected to nuclear envelope &
extends throughout cell
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AP Biology
Types of ER
rough smooth
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AP Biology
Smooth ER function Membrane production Many metabolic processes
synthesis synthesize lipids
oils, phospholipids, steroids & sex hormones
hydrolysis hydrolyze glycogen into glucose
in liver
detoxify drugs & poisons in liver ex. alcohol & barbiturates
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AP Biology
Membrane Factory Build new
membrane synthesize
phospholipids builds membranes
ER membrane expands bud off & transfer
to other parts of cell that need membranes
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AP Biology
Rough ER function Produce proteins for export out of cell
protein secreting cells packaged into transport vesicles for export
Which cellshave lot of rough ER?
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AP Biology
Synthesizing proteins
cytoplasm
cisternalspace
mRNA
ribosome
membrane ofendoplasmic reticulum
polypeptide
signalsequence
ribosome
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AP Biology
Golgi Apparatus
Which cellshave lots of Golgi?
transport vesicles
secretoryvesicles
Function finishes, sorts, tags & ships cell products
like “UPS shipping department” ships products in vesicles
membrane sacs “UPS trucks”
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AP Biology
Golgi Apparatus
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AP Biology
Vesicle transport
vesiclebuddingfrom roughER
fusionof vesiclewith Golgiapparatus
migratingtransportvesicle
protein
ribosome
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Regents Biology
DNA
RNA
ribosomes
endoplasmicreticulum
vesicle
Golgi apparatus
vesicle
proteinon its way!
protein finishedprotein
Making Proteins
TO:
TO:
TO:
TO:
nucleus
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AP Biology
proteins
transportvesicle
Golgiapparatus
vesicle
smooth ER
rough ER
nuclear porenucleus
ribosome
cellmembrane protein secreted
cytoplasm
Making proteinsPutting it together…
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AP Biology
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AP Biology
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2007-2008AP Biology
Any Questions!!