Optical Biosensors Joel Kindt Lauren Netherton Acknowledgements Dr. Kevin Lear Dr. Dave Kisker Weina...
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Transcript of Optical Biosensors Joel Kindt Lauren Netherton Acknowledgements Dr. Kevin Lear Dr. Dave Kisker Weina...
Optical Biosensors
Joel Kindt
Lauren Netherton
Acknowledgements
Dr. Kevin Lear
Dr. Dave Kisker
Weina Wang
Hailey Cutler
1
Lauren Netherton
• Motivation and Introduction
• Prior Research
• How Optical Biosensors Work
2
3Courtesy of Kian Behbakht of UC Denver
Prior Research
Senior Design Team 2007-2008
4
Pipe Organ Analogy
• Place fruit in organ pipe
• Changes pitch based on:– Size– Shape
http://lib.colostate.edu/archives/phelps/
5
6
Optical Detection & RF Switch
Circuit Spectrometer
HealthyCancerous!
Courtesy of Weina Wang
Trapping Requirements
• Lower voltages– Trap at
slower velocity
• Higher voltages– Trap at
higher velocity
7
Dielectrophoretic Force
• Use of electromagnetics• Non-uniform electric field
– Polarizes molecules within cell– Induces DEP force to hold cell in trap
Electric field
8
December 2007 Senior Design Presentation
3 22 Re[ ]DEP mF r K E
Polarization factor (K factor):* *
* *2p m
p m
K
* j
Complex permittivity of particle/suspending medium:
Dielectrophoretic Force
9Courtesy of Weina Wang
Joel Kindt
• Electrostatic Modeling
• Experimental Data
• Budget
• Conclusions and Future Work
10
Electrodes on Chip
11
Electrostatic Modeling
12
3 22 Re[ ]DEP mF r K E
DEP ForceE-Field
13
Positive and Negative DEP
• Mammalian white blood cell
• σm = 0.1 S/m (~ water)
• σm = 1.5 S/m (~ PBS)
3 22 Re[ ]DEP mF r K E
Joel Voldman, “Electrical Forces For Microscale Cell Manipulation”, Annu. Rev. Biomed. Eng. 2006. 8:425-54
14
Polarization factor (K factor):* *
* *2p m
p m
K
K > 0: Positive DEP K < 0: Negative DEP
* j
,
Experimental Setup
15
Impedance of Channel
Voltage Divider
http://en.wikipedia.org/wiki/Voltage_divider16
more heat dissipated at lower impedance
17
Impedance of Channel
Budget
Materials 1st semesterbudget
1st semesteractual
2nd semesterbudget
Syringes $15 $0 $15
Glass $75 $0 $0
Nanoports $0 $0 $60
Nanotubing $0 $0 $25
Flexible mirror $10 $0 $0
Total $100 $0 $100
18
• Know trapping requirements for various fluid velocities
• Possible to model cell trap through simulations– Electric field
– DEP force
• The fluid properties can affect results– K factor
– Impedance of channel19
Conclusions
• Design better cell trap through simulations
• Further understand spectra– Cancerous vs. non-cancerous cells
– Different types of cancer
• Save Rover!
20
Future Work