Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235...
-
Upload
abigayle-anne-marshall -
Category
Documents
-
view
224 -
download
0
Transcript of Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235...
![Page 1: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/1.jpg)
Onur Ergen
“Flexible electrical recording from cells using nanowire transistor arrays”
EE235
Student Presentation 24 may 2009
Electirical Engineering and computer science University of California, Berkeley
![Page 2: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/2.jpg)
Outline
Advantage of using NWs for establishing active interfaces with biological systems.
Fabrication and transfer method of NWs.
Discussion on experimental approach and electrical results of cardiomyocyte signals.
Conclusion
![Page 3: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/3.jpg)
![Page 4: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/4.jpg)
Fabrication and transfer method of NWs
Synthesis of semiconductor silicon nanowires. Scheme of nanowire synthesis set-up.
Transfer of Nws with using contact printing.
•Javey A, Nam S, Friedman RS, Yan H, Lieber CM (2007) Layer-by-layer assembly of nanowires for three-dimensional, multifunctional electronics. Nano Lett 7:773–777.•Patolsky F, Zheng G, Lieber CM (2006) Fabricaton of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protocols 1:1711–1724. A P
![Page 5: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/5.jpg)
Schematic of Experimental Approach
◦ NWFET arrays coupled to embryonic chicken cardiomyocytes.
A-) NWFET chip, where NW devices are located at central region of chip. Source and 2 drain electrodes connected to a vertically oriented NW.
B-) Cardiomyocytes cultured on thin rectangular pieces of PDMS.
C-) PDMS piece with cultured cells oriented over the device region of NWFET chip.
D-)Schematic view of a cardiomyocyte oriented over a NW FET device.
Embryonic chicken
cardiomyocytes.
![Page 6: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/6.jpg)
Measurement of Cardiomyocyte Signals
retracted Back into contact
--Measurement of cardiomyocyte signals----Effect of applied force on recorded signals--
![Page 7: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/7.jpg)
Previous Approach ; Electrical Recording from Hearts with Flexible Nanowire Device Arrays
B.P. Timko, T. Cohen-Karni, G. Yu, Q. Qing, B. Tian and C.M. Lieber, "Electrical Recording from Hearts with Flexible Nanowire Device Arrays," Nano Lett. 9, 914-918 (2009).
![Page 8: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/8.jpg)
Measurement of Cardiomyocyte Signals
--Multiplexed Recording----Recording from Distinct Regions--
![Page 9: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/9.jpg)
Conclusions
◦ Good flexible approach to interface silicon NWFETs with cells in which optically transparent PDMS/cell substrates are manipulated to place specific cells over particular NWFET elements.
◦ Amplitude variations signals of cardiomyocytes from 31 to 7 nS for different Vg values recorded (while keeping from calibrated voltage remained constant ).
◦ Multiplexed recording of signals shows cardiomyocyte monolayers enabled temporal shifts and signal propagation.
◦ Approach increases the flexibility and opens up opportunities for interfacing cardiomyocytes and other cells to high-performance NWFETs,
![Page 10: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/10.jpg)
Supplementary
•Javey A, Nam S, Friedman RS, Yan H, Lieber CM (2007) Layer-by-layer assembly of nanowires for three-dimensional, multifunctional electronics. Nano Lett 7:773–777.
![Page 11: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/11.jpg)
Transport measurement of SiNW devices in air
Nws transfer on Si substrate by drop-cast
•Patolsky F, Zheng G, Lieber CM (2006) Fabricaton of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat Protocols 1:1711–1724. A P
![Page 12: Onur Ergen “Flexible electrical recording from cells using nanowire transistor arrays” EE235 Student Presentation 2 4 may 2009 Electirical Engineering.](https://reader035.fdocuments.in/reader035/viewer/2022062315/5697c00e1a28abf838cc9ed8/html5/thumbnails/12.jpg)