Only ONE Change to Dramatically Improve

SunFire™ METHODS

Waters®

Core-Shell Technology

SunFire is a trademark and Waters is a registered trademark of Waters Corporation (Milford, MA, USA). Phenomenex is not affiliated with Waters Corporation.

The simple change

is…

0

0

100

200

mAU

20860

2 4 6 8 10 min

a

b c d*

0 2 4 6 8 10 12 14 min

a

b c d

0

100

mAU

20859

*

20737

0 2 4 6 8 10 12 min

1

2+3

4

5

6 7

89

10

11

12

13

0

100

mAU

Kinetex 5 µm core-shell columns provide considerable improvements for methods devel-oped on standard HPLC systems with conventional 3 µm or 5 µm fully porous columns - at low backpressures.

SunFire is a trademark and Waters is a registered trademark of Waters Corporation. (Milford, MA, USA). Phenomenex is not affili-ated with Waters Corporation. Comparative separations may not be representative of all applications.

…Replace your 3.5 or 5 µm Waters® SunFire™ Column with a

NEW Kinetex 5 µm Core-Shell HPLC Column

™

5 µm

Conditions for all columns:Columns: Kinetex 5 µm C18

SunFire 3.5 µm C18Dimensions: 150 x 4.6 mm

Mobile Phase: A: Water with 0.1% TFA B. Acetonitrile with 0.1% TFA

Gradient: Time (min)0 15

% B5 95

Flow Rate: 1.2 mL/minTemperature: Ambient

Detection: UV @ 254 nmSample: Pharmaceutical Sample

1. Procainamide 2. Acetaminophen 3. Folic acid 4. Sulfathiazole 5. Acebutolol 6. Dextromethorphan 7. Diphenhydramine 8. Propafenone 9. Amitriptyline 10. Fluoxetine 11. Naproxen 12. Diflunisal 13. Indomethacin

Better Chromatographic Performance• Higher Efficiency

• Improved Sensitivity

• Increased Resolution

Increased Resolution and Sensitivity by substituting fully porous column with Kinetex 5 µm

Higher Productivity by then reducing column length

Lower Costs by lastly adjusting gradient profile proportionally

20738

0 2 4 6 8 10 12 min

1

2

3

4

5

6 7

89

10

11

12

13

0

100

mAU

Phenomenex Kinetex® 5 µm C18

Waters® SunFire™ 3.5 µm C18

120 Bar

220 Bar

Ap

p ID

207

38A

pp

ID 2

0737

a

b c d0

20

40

60

80

100

120

140

160mAU

20766

0 10 20 min

*0

20

40

60

80

100

120

140

160mAU

20767

0 10 20 min

a

b c d*

Conditons for all columns, except where notedColumn: Kinetex 5 µm C18

Fully porous 5 µm C18Dimensions: 250 x 4.6 mm

Mobile Phase: A: TFA/Water/THF (5/900/100) B: TFA/Acetonitrile/THF (5/900/100)

Gradient: Time (min) % B 0 10 40 80

Flow Rate: 1.5 mL/minTemperature: 40 °C

Detection: UV @ 295 nmSample: 1. Impurity a 4. Unidentified (*)

2. Impurity b 5. Paroxetine3. Impurity c 6. Impurity d

Ap

p ID

207

66

Ap

p ID

207

67

Kinetex® 5 µm XB-C18

Kinetex® 5 µm XB-C18

Fully Porous 5 µm C18

Kinetex® 5 µm XB-C18

Dimensions: 150 x 4.6 mm Dimensions: 150 x 4.6 mmGradient: Time (min) % B

0 10 24 80

Critical Advantages of Increased Resolution:

• Save time and money by adjusting method

• Separate critical pairs or difficult mixtures

• Less risk of failing system suitability requirements

Ap

p ID

208

59

Ap

p ID

208

60

• Shorter run time

• More runs per day• Less solvent used• Reduce disposal

costs

Drop-in a Kinetex 5 µm Columnand instantly attain . . .

Drop-in Kinetex 5 µm + Simple Method Adjustmentsto achieve...

OR

See Kinetex 5 µm on Your Method In less than 2 minutes, the Kinetex 5 µm Calculator computes the performance and cost benefits achievable by replacing your fully porous column.

http://www.phenomenex.com/methodmakeover

No co-elution

Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com

Only ONE Change to Dramatically Improve

SunFire™ METHODS

Waters®

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

Trademarks Phenomenex and Kinetex are registered trademarks, SecurityGuard is a trademark of Phenomenex. SunFire is a trademark and Waters is a registered trademark of Waters Corporation.

Disclaimer Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications.

© 2013 Phenomenex, Inc. All rights reserved.FL98

3011

12_W

Kinetex® 5 µm Columns (mm)

SecurityGuard™ ULTRA Cartridges*50 x 2.1 3/pk

Phases XB-C18 00B-4605-AN AJ0-8782C18 00B-4601-AN AJ0-8782PFP 00B-4602-AN AJ0-8787Phenyl-Hexyl 00B-4603-AN AJ0-8788

for 2.1 mm ID

SecurityGuard ULTRA Cartridges*50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 3/pk

Phases XB-C18 00B-4605-E0 00D-4605-E0 00F-4605-E0 00G-4605-E0 AJ0-8768C18 00B-4601-E0 00D-4601-E0 00F-4601-E0 00G-4601-E0 AJ0-8768PFP 00B-4602-E0 00D-4602-E0 00F-4602-E0 00G-4602-E0 AJ0-8773Phenyl-Hexyl 00B-4603-E0 00D-4603-E0 00F-4603-E0 00G-4603-E0 AJ0-8774* SecurityGuard ULTRA cartridges require holder, Part No. AJ0-9000. Kinetex 5 µm columns are also available in 21.2 mm ID.

for 4.6 mm ID

If Kinetex core-shell columns do not pro-vide at least an equivalent separation as compared to a competing column of the same phase, return the column with the comparative data within 45 days for a FULL REFUND.