Omega 5 Frequently Asked Questions
2
1 2 3 4 OMEGA AU 0 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 1.5 2 2.5 min App ID 23737 TMS TMS TMS TMS + + OMEGA OMEGA OMEGA OMEGA USP:L1 0 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 1.5 2 2.5 min Adenine G-5-MP Thymine 100% 80% 60% 40% 20% 0% App ID 23577 Comparative separations may not be representative of all applications. Comparative separations may not be representative of all applications. 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 1.5 2 2.5 min AU AU AU AU App ID 23738 App ID 26100 App ID 26101 App ID 26102 TMS TMS U S L 0.00 100.00 200.00 300.00 400.00 500.00 600.00 700.00 2 1 2 3 4 5 6 7 8 10 12 11 9 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 min Analyte Retention Time USP Resolution 7 Mannitol 20.80 1.69 8 Sucrose 24.58 16.50 9 Maltose 25.58 4.30 10 Maltitol 25.90 1.39 11 Lactose 26.34 2.15 12 Lactitol 26.96 2.94 Analyte Retention Time USP Resolution 1 2 3 4 5 6 Glycerol 6.59 Erythritol 9.31 13.32 Xylitol 13.88 16.01 Fructose 15.43 3.87 Glucose 19.51 6.74 Sorbitol 20.26 1.36 2 4 6 App ID 25582 Batch A Batch B Batch C PEAK Reproducibility (batch-to-batch, column-to-column) By setting a new standard for reliability, the Luna Omega C18 spans UHPLC and HPLC with a scalable range of high-performance particle sizes that will ensure that your developed methods are easily transferred. From single compound identification to complex impurity profiles, the Luna Omega C18 will serve as a pillar for your lab to count on day in and day out. Luna ® Omega 5 Frequently Asked Questions Question 1: Are the Luna Omega stationary phases stable with a 100 % aqueous mobile phase? Question 2: How does the loadability of Luna Omega compare to other fully porous stationary phases? The Luna Omega Polar C18, Luna Omega PS C18, and Luna Omega SUGAR columns are all 100 % aqueous stable. The Luna Omega C18 requires a minimum of 5 % organic solvent in the mobile phase to prevent phase de-wetting. A thermal treatment process during the manufacturing of the Luna Omega silica particles does reduce the relative surface area of the material, however, this reduction does not negatively impact the loadability. This is because the thermal treatment reduces the presence of micropores rather than accessible particle porosity. As such, Luna Omega stationary phases provide excellent loadability in comparison to other fully porous stationary phase materials. Question 3: Any advice on how to improve method reproducibility? Method reproducibility is a result of many different variables and it is best practice to conduct multi-batch validations. This allows any unforeseen interactions and method sensitivity to become apparent. Additionally, using newer silica technology columns like Luna Omega C18 that have been optimized for reproducibility will also help to minimize the negative effect of unwanted secondary interactions. For example, here is a demonstration of a multi-batch drug impurity profile. Question 4: Are any of the Luna Omega columns suitable for HILIC separations of very polar compounds? Yes! The Luna Omega SUGAR is comprised of a combined amide polyol/amine-based phase with aqueous stable end-capping to offer enhanced HILIC retention of polar compounds such as sugars and sugar alcohols through multiple interaction mechanisms. Plus Luna Omega SUGAR is tested by HILIC conditions for sugars to ensure it is high quality! Better Peak Shape Over All Loads Aqueous Stability of Luna Omega Polar C18 Comparative separations may not be representative of all applications. Duration of Exposure Waters ® ACQUITY ® BEH 1.7 μm C18 Amitriptyline Loading Study Luna Omega 1.6 µm PS C18 Day 1 Day 25 Peak Identification and Respective Resolution Values Chromatogram of 12 Polyols and Simple Sugars with the Luna Omega SUGAR Column % of Initial Retention Time Promethazine HCL and its five organic impurities, reproducibly separated across 3 different batches of Luna Omega 5 μm C18. Conditions for all columns: Columns: Luna Omega 1.6 μm PS C18 ACQUITY BEH 1.7 μm C18 Dimensions: 50 x 2.1 mm Mobile Phase: A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid Gradient: Time (min) 0 5 % B 5 80 Flow Rate: 0.4 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. Amitriptyline (0.02 µg) 2. Amitriptyline (0.04 µg) 3. Amitriptyline (0.06 µg) 4. Amitriptyline (0.08 µg) 5. Amitriptyline (0.10 µg) 6. Amitriptyline (0.15 µg) 7. Amitriptyline (0.20 µg) 8. Amitriptyline (0.30 µg) 9. Amitriptyline (0.40 µg) 10. Amitriptyline (0.5 µg) Columns: Luna Omega 1.6 µm Polar C18 Dimension: 50 x 2.1 mm Part No.: 00B-4748-AN Mobile Phase: 10 mM Ammonium Formate with 0.1 % Formic Acid Flow Rate: 0.4 mL/min Temperature: 25 °C Detection: UV @ 254 nm Sample: 1. Adenine 2. Guanosine-5-Monophosphate 3. Thymine Column: Luna Omega 5 µm C18 Dimensions: 250 x 4.6 mm Part No.: 00G-4785-E0 Mobile Phase: A: Acetonitrile and buffer (3.7 g/L ammonium acetate in water, 300:700) B: Acetonitrile Gradient: Time (min) % B 0 0 10 18 18.1 25 40 40 0 0 Flow Rate: 1.4 mL/min Injection Volume: 15 µL Temperature: 30 °C Detection: UV @ 249 nm (ambient) USA Tel: 310-212-0555 | Canada Tel: 800-543-3681 | Puerto Rico Tel: 800-541-4752 | Email: [email protected] 0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 min 1 2 3 4 5 0 5 10 15 20 25 30 min 0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 0 5 10 15 20 25 30 0 2.5 5 1 2 3 4 5 7.5 10 12.5 15 17.5 20 22.5 min 0 5 10 15 20 25 30
Transcript of Omega 5 Frequently Asked Questions
1
2
3
4
OMEGA
AU
0
0.10
0.20
0.30
0.40
0.50
0.60
0.70
0.80
1.5 2 2.5 min
App
ID 2
3737
TMSTMS TMSTMS
++
OMEGA
OMEGA
OMEGA
OMEGA
USP:L10
0.10
0.20
0.30
0.40
0.50
0.60
0.70
0.80
1.5 2 2.5 min
Adenine G-5-MP Thymine
100%110%
80%
60%
40%
20%
0% App
ID 2
3577
Comparative separations may not be representative of all applications.
Comparative separations may not be representative of all applications.
0.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
0.80
1.5 2 2.5 min
AU
AU
AU
AU
App
ID 2
3738
App
ID 2
6100
App
ID 2
6101
App
ID 2
6102
HILIC
TMSTMS
USL
0.00
100.00
200.00
300.00
400.00
500.00
600.00
700.00
2
1
23 4
5
67
8 10
12
11
9
4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 min
Analyte Retention Time USP Resolution
7 Mannitol 20.80 1.69
8 Sucrose 24.58 16.50
9 Maltose 25.58 4.30
10 Maltitol 25.90 1.39
11 Lactose 26.34 2.15
12 Lactitol 26.96 2.94
Analyte Retention Time USP Resolution
1
2
3
4
5
6
Glycerol 6.59
Erythritol 9.31 13.32
Xylitol 13.88 16.01
Fructose 15.43 3.87
Glucose 19.51 6.74
Sorbitol 20.26 1.36
2
4
6
App
ID 2
5582
Batch A
Batch B
Batch C
PEAK Reproducibility (batch-to-batch, column-to-column)By setting a new standard for reliability, the Luna Omega C18 spans UHPLC and HPLC with a scalable range of high-performance particle sizes that will ensure that your developed methods are easily transferred. From single compound identification to complex impurity profiles, the Luna Omega C18 will serve as a pillar for your lab to count on day in and day out.
Luna® Omega 5 Frequently Asked Questions Question 1: Are the Luna Omega stationary phases stable with a 100 % aqueous mobile phase?
Question 2: How does the loadability of Luna Omega compare to other fully porous stationary phases?
The Luna Omega Polar C18, Luna Omega PS C18, and Luna Omega SUGAR columns are all 100 % aqueous stable. The Luna Omega C18 requires a minimum of 5 % organic solvent in the mobile phase to prevent phase de-wetting.
A thermal treatment process during the manufacturing of the Luna Omega silica particles does reduce the relative surface area of the material, however, this reduction does not negatively impact the loadability. This is because the thermal treatment reduces the presence of micropores rather than accessible particle porosity. As such, Luna Omega stationary phases provide excellent loadability in comparison to other fully porous stationary phase materials.
Question 3: Any advice on how to improve method reproducibility?
Method reproducibility is a result of many different variables and it is best practice to conduct multi-batch validations. This allows any unforeseen interactions and method sensitivity to become apparent. Additionally, using newer silica technology columns like Luna Omega C18 that have been optimized for reproducibility will also help to minimize the negative effect of unwanted secondary interactions. For example, here is a demonstration of a multi-batch drug impurity profile.
Question 4: Are any of the Luna Omega columns suitable for HILIC separations of very polar compounds?
Yes! The Luna Omega SUGAR is comprised of a combined amide polyol/amine-based phase with aqueous stable end-capping to offer enhanced HILIC retention of polar compounds such as sugars and sugar alcohols through multiple interaction mechanisms. Plus Luna Omega SUGAR is tested by HILIC conditions for sugars to ensure it is high quality!
Better Peak Shape Over All Loads
Aqueous Stability of Luna Omega Polar C18
Comparative separations may not be representative of all applications.
Duration of Exposure
Waters® ACQUITY® BEH 1.7 μm C18
Amitriptyline Loading Study
Luna Omega 1.6 µm PS C18
Day 1
Day 25
Peak Identification and Respective Resolution Values
Chromatogram of 12 Polyols and Simple Sugars with the Luna Omega SUGAR Column
% o
f In
itial
Ret
entio
n T
ime
Promethazine HCL and its five organic impurities, reproducibly separated across 3 different batches of Luna Omega 5 μm C18.
Conditions for all columns:Columns: Luna Omega 1.6 μm PS C18
ACQUITY BEH 1.7 μm C18Dimensions: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic AcidB: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min)05
% B580
Flow Rate: 0.4 mL/minTemperature: 22 °C
Detection: UV @ 254 nmSample: 1. Amitriptyline (0.02 µg)
2. Amitriptyline (0.04 µg)3. Amitriptyline (0.06 µg)4. Amitriptyline (0.08 µg)5. Amitriptyline (0.10 µg)6. Amitriptyline (0.15 µg)7. Amitriptyline (0.20 µg)8. Amitriptyline (0.30 µg)9. Amitriptyline (0.40 µg)
10. Amitriptyline (0.5 µg)
Columns: Luna Omega 1.6 µm Polar C18Dimension: 50 x 2.1 mm
Part No.: 00B-4748-ANMobile Phase: 10 mM Ammonium Formate with
0.1 % Formic AcidFlow Rate: 0.4 mL/min
Temperature: 25 °CDetection: UV @ 254 nm
Sample: 1. Adenine2. Guanosine-5-Monophosphate3. Thymine
Column: Luna Omega 5 µm C18 Dimensions: 250 x 4.6 mm
Part No.: 00G-4785-E0Mobile Phase: A: Acetonitrile and buffer (3.7 g/L
ammonium acetate in water, 300:700)B: Acetonitrile
Gradient: Time (min) % B0 010 18 18.1 25
40 40 0 0
Flow Rate: 1.4 mL/minInjection Volume: 15 µL
Temperature: 30 °CDetection: UV @ 249 nm (ambient)
USA Tel: 310-212-0555 | Canada Tel: 800-543-3681 | Puerto Rico Tel: 800-541-4752 | Email: [email protected]
0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 min
1 2
34
5
mAU
0
5
10
15
20
25
30
min0 2.5 5 7.5 10 12.5 15 17.5 20 22.5
mAU
0
5
10
15
20
25
30
0 2.5 5
1 2
3 4
5
7.5 10 12.5 15 17.5 20 22.5 min
mAU
0
5
10
15
20
25
30
OMEGA
OMEGA
5
OMEGA
TMSTMS
NEW
Your happiness is our mission. Take45 days to try our products. If you are not happy, we’ll make it right.
guarantee www.phenomenex.com/behappy
Luna® Omega 5 Frequently Asked Questions
Luna Omega Ordering Information
USP: L1
TMSTMS
PolarPolar
HydrophobicitySteric Interaction
Hydrogen Bond Donating CapacityHydrogen Bond Accepting Capacity
Cation Selectivity at pH 2.8Cation Selectivity at pH 7.0
Low High
USP: L1
TMSTMS
Luna Omega C18
TMSTMS
++TMSTMS
Luna Omega PS C18
PolarPolar
TMSTMS
Luna Omega Polar C18
5 Frequently Asked Questions About Luna Omega HPLC/UHPLC Columns
5 Frequently Asked Questions AboutLuna Omega HPLC/UHPLC Columns
Question 5: What is the difference between Luna Omega C18, Polar C18, and PS C18?
The combination of available Luna Omega stationary phases represent an outstanding tool set for the separation of acids, bases, neutrals or mixtures. Use the C18 to focus on hydrophobic interactions or the multi-interaction functionality of the Polar C18 and PS C18 to get enhanced retention of both polar and non-polar compounds.
USP: L1
TMSTMS
++
HydrophobicitySteric Interaction
Hydrogen Bond Donating CapacityHydrogen Bond Accepting Capacity
Cation Selectivity at pH 2.8Cation Selectivity at pH 7.0
Low High
* pH stability under gradient conditions. pH stability is 1.5 - 10 under isocratic conditions.
** 1.6 µm Luna Omega columns are pressure stable up to 1034 bar and 3 or 5 µm are stable up to 345 bar.
Material Characteristics
PhaseParticle
Sizes (µm)Pore Size
(Å)Effective Surface
Area (m²/g)Effective
Carbon Load %pH
StabilityPressure Stability
Luna Omega PS C18 1.6, 3, 5 100 260 9 1.5 - 8.5*
1,034/345† barLuna Omega Polar C18 1.6, 3, 5 100 260 9 1.5 - 8.5*
Luna Omega C18 1.6, 3, 5 100 260 11 1.5 - 8.5*
Phenomenex.com/LunaOmega
5 µm Minibore and MidBore Columns (mm) SecurityGuard Cartridges (mm)
Phases 50 x 2.1 100 x 2.1 150 x 2.1 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0*
/10 pkPolar C18 00B-4754-AN 00D-4754-AN 00F-4754-AN 00B-4754-Y0 00D-4754-Y0 00F-4754-Y0 AJ0-7600PS C18 00B-4753-AN 00D-4753-AN 00F-4753-AN 00B-4753-Y0 00D-4753-Y0 00F-4753-Y0 AJ0-7605
for ID: 2.0 - 3.0 mm
5 µm Analytical Columns (mm) SecurityGuard
Cartridges (mm)Phases 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0*
/10 pkPolar C18 00B-4754-E0 00D-4754-E0 00F-4754-E0 00G-4754-E0 AJ0-7601PS C18 00B-4753-E0 00D-4753-E0 00F-4753-E0 00G-4753-E0 AJ0-7606C18 00B-4785-E0 00D-4785-E0 00F-4785-E0 00G-4785-E0 AJ0-7612
for ID: 3.2-8.0 mm‡ SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000
* SecurityGuard Standard Analytical Cartridges require holder, Part No.: KJ0-4282
1.6 µm Minibore Columns (mm) SecurityGuard™
ULTRA CartridgesPhases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 3/pk‡
Polar C18 00A-4748-AN 00B-4748-AN 00D-4748-AN 00F-4748-AN AJ0-9505PS C18 00A-4752-AN 00B-4752-AN 00D-4752-AN 00F-4752-AN AJ0-9508C18 00A-4742-AN 00B-4742-AN 00D-4742-AN 00F-4742-AN AJ0-9502
for 2.1 mm ID
3 µm Analytical Columns (mm)SecurityGuard
Cartridges (mm)Phases 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0*
/10 pkPolar C18 00B-4760-E0 00D-4760-E0 00F-4760-E0 00G-4760-E0 AJ0-7601PS C18 00B-4758-E0 00D-4758-E0 00F-4758-E0 00G-4758-E0 AJ0-7606C18 00B-4784-E0 00D-4784-E0 00F-4784-E0 00G-4784-E0 AJ0-7612SUGAR — 00D-4775-E0 00F-4775-E0 00G-4775-E0 AJ0-4495
for ID: 3.2-8.0 mm
3 µm Minibore and MidBore™ Columns (mm) SecurityGuard
Cartridges (mm)Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0*
/10 pkPolar C18 00A-4760-AN 00B-4760-AN 00D-4760-AN 00F-4760-AN 00B-4760-Y0 00D-4760-Y0 00F-4760-Y0 AJ0-7600PS C18 00A-4758-AN 00B-4758-AN 00D-4758-AN 00F-4758-AN 00B-4758-Y0 00D-4758-Y0 00F-4758-Y0 AJ0-7605C18 — 00B-4784-AN 00D-4784-AN 00F-4784-AN 00B-4784-Y0 00D-4784-Y0 00F-4784-Y0 AJ0-7611SUGAR — 00B-4775-AN 00D-4775-AN 00F-4775-AN — — 00F-4775-Y0 AJ0-4496
for ID: 2.0-3.0 mm
1.6 μm Microbore Columns (mm)Phases 50 x 1.0 100 x 1.0 150 x 1.0Polar C18 00B-4748-A0 00D-4748-A0 00F-4748-A0C18 00B-4742-A0 00D-4742-A0 00F-4742-A0PS C18 — 00D-4752-A0 —
Luna Omega Polar C18
Luna Omega PS C18
Luna Omega C18
Luna Sugar
Aq AqAmino
Amide Polyol
NEW
NEW
NEW
0 1 2 3 4 5 6 7 8 9 10 min
0
0.05
0.10
0.15
0.20
0.25
0.30
0.35
Inte
nsity
, cp
s Useful alternative peak elution order
Inte
nsity
, cp
s
0 1 2 3 4 5 6 7 8 9 10 min
0
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0 1 2 3 4 5 6 7 8 9 10 min
0
0.05
0.10
0.15
0.20
0.25
0.30
0.35
Inte
nsity
, cp
s HydrophobicitySteric Interaction
Hydrogen Bond Donating CapacityHydrogen Bond Accepting Capacity
Cation Selectivity at pH 2.8Cation Selectivity at pH 7.0
Low High
App
ID 2
3741
App
ID 2
3743
App
ID 2
3744
Terms and ConditionsOffers valid only for addressee. Offers must be specified at time of order and cannot be combined with any other discount or promotional item. All discounts valid for Luna Omega C18 and Kinetex C18 columns only. Discount excludes accessories, columns > 10 mm ID, and custom columns. Subject to Phenomenex standard terms & conditions, which may be viewed atwww.phenomenex.com/TermsAndConditions.
TrademarksLuna and Kinetex are registered trademarks and SecurityGuard, BE-HAPPY, and MidBore are trademarks of Phenomenex.Waters and ACQUITY are registered trademarks of Waters Technologies Corporation.
DisclaimerPhenomenex is not affiliated with Waters Technologies Corporation. Comparative separations may not be representative of all applications.
SecurityGuard is patented by Phenomenex. U.S. Patent No. 6,162,362.CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges.
FOR RESEARCH USE ONLY. Not for use in clinical diagnostic procedures.
© 2020 Phenomenex, Inc. All rights reserved.
Now Available!
Micro 0.3 & 0.5 mm ID Luna Omega columns
Ask for the guide at:www.phenomenex.com/chatnow
PC
7599
0920
_us
