Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top...

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System Microscopes

Transcript of Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top...

Page 1: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

System Microscopes

Page 2: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BH2 Series System Microscope The BH2 Series of topquality microscopes facilitates the use of a wide range of microscopic techniques, depending on the choice of accessories. The Koehler-type illumination elicits the full performance of the LB Series objectives, yielding photomicrographs of the highest quality. This series of microscopes is suitable for a very wide range of applications, from routine laboratory work to educational and research investigations.

Page 3: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

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Wandlng -v The design of the entire system--microscope stands, observation tubes, stages, etc.-bears witness to creative and inventive thinking. One example may be seen in the design of the coarse stage adjustment, in which movement is limited in the upward direction in order to prevent contact between objective and specimen. The coarse and fine focusing knobs are of coaxial design, and the mini- mum increment on the fine adjustment knob represents a stage mwernent of just two microns. The coaxial stage control knobs are located close to the focusing knobs, low enough to permit operation witt~wt having to raise the arm off the arm rest. In addition, the BH2-TR30 trinocular observa- tion tube features constant tube length adjustment which allows camera focus

through the binocular tube in phatamicrb graphy, thereby adding significantly to ease of operation. Improved Illumlnatlng System All BH2 Series microscopes employ the widely accepted LB Series objectives and WHKl OX eyepieces (as standard equipment). providing F.N. 20 widefield observation. BH2 Series microscopes provide Koehler illumination to exploit the full benefits of the LB Series objectives. A wide choice of condensers provides uniform illumina:icm from ultra low to high magnifications. These microscopes incorporate precentered halogen lamps, aspherical collectors and fully enclosed light paths designed to keep out dirt and dust.

Page 4: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination for a wide range of applications. It is ideally suited for advanced research work and investigatians requiring high precision.

Page 5: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BHSF Mhwcqm Stand .The BHS-F stand is of a robust and stable design unaffected by vibration or shaking. Highly reliable, it is ideal for photomicro graphic purposes.

.The stand incorporates a h i g h p e r f o m transformer which is unaffected by fluctua- tions in the line vdtage and so always provides light of uniform brightness and mlor temperature.

.The ball bearing sextuple revohring nose piece accepts law to high magnification objectives with 45mm parfocal distance. This feature contributes significantly to greater efticiency in microscopy.

BHSLSH Halogen Lamp Housing The IN l W halogen lamp provides a bright light source suitable for many types of microscopy. The precentered lamp has an average life of around 2,000 hours, reducing slgnlflcantly the time lml with troublesome bulb change.

Page 6: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BHT System Microscope Employing the same revoking nosepieces, stages, observation tubes, etc., as the Model BHS, the Model BHT microscope uses a different type of illumination system and electrical components. A compact microscope, it offers excellent cost-performance.

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BHFF Mkmscmp Stand *Smaller than the BHS model, with a different transformer arKl lighl source. Extremely functional.

*The dust proof illuminating system keeps the light path clean.

LS#HI Lamp H W n g Encloses a precentersd 6V 20W halogen t .

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LB (Long Barrel) Objectives The LB (Long Barrel) Series ranges from inexpensive objectives to top quality and special-purpose objectives. Thls extensive selection enables the user to choose the ideal lens for the purpose intended.

In the design of LB objectives, OLYMPUS has drawn upon its extensive and renowned expertise In optical technology. The objectives offer the following features: 1 . High resolution 2. Superior contrast 3. Excellent field flatness 4. F.N. 20 widefield observation (with

WHKl OX eyepiece) 5. Ultra-!ow magnification objectives are

parfocal wtth high-magnification lenses.

S Plan Apodumat Objactlm These objectives are of the highest quality design. Chromatic aberration is thoroughly compensated, field flatness is superb and the high numerical aperture provides outstanding resolution.

S Plan Aohmmat ObJechs The S Plan objectives are the most popular high quality objectives in the LB series. Excellent optical correction across the entire lens surfaces makes them ideally suited for use in differential interference contrast microscopy and for super widefield observation. S Plan FL ObJacthres The ultra low magnifications (lX, 2X) of these objectives make them ideal far examinatkm of large specimen areas. They are fully parfocal with other LB cbjet i is . The SRanFL2X objective is suitable for super widefieid otservatlons. Note: The objectives prform bast with the BHPULC

ultra-low condenser.

D Plan Achromat O b J e d w With D Plan objectives, fleld flatness is guaranteed up to F.N. 20. Ideal for photo micrographic purpses, they are widely used in research, educational and routlne work. D mrolmt Objective8 These are the most economically priced objectives in the LB series. Resolution and flatness in the center portion of the field of view are excellent and they are particularly suitable for routine work, educational purposes or training. N o ~ O b ~ This type of abjective is ideal for examination of specimens with no cover glass, such as blood smears. 'A varlety 01 other types of objectim are available for dlffarent applicatOwts and requirements, including p h m t r a s t , reflected light flwrescmce, or polarked light microscopy.

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Page 9: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

Eyepieces and Condensers To complement the performance of our LB objectives, an extensive range of eyepieces and six types of condensers are available. To exploit the full performance of objectives with magnifications from IX to lOOX requires the choice of the proper condenser.

WHKlNK Eyepieces I h

LB Serbs Eyepieces Designed for use with LB Series objectives, WHK eyepieces are easy and comfortable to use and are of widefield W i n (F.N. 20 with the WHKlOX eyepiece). The high eye point makes them easy to use by persons who wear glasses and enables fatiguefree observation over long periods of time. Other eyepieces in the series include the WHK8X, WHK15X, NK5X and NK20X. NFK Serks Photo Eyapkcss These photo eyepieces are speclflcally designed for photomicrography. They are available in 2.5X, 3.3X, 5X and 6.7X magnifications.

condemm An extensive range of condensers is available, covering brightfield and darkfield observa- tions. The BH2-AAC is a top quality achromatic aplanatic brightfield condenser which permits full compensation for all aberrations. The swingat BH2-SC condenser is suitable for a wide range of magnifications. Several other condensers are available, including the general-purpose BH2CD and the BH2-ULC for ultra-low magnification.

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Observation Tubes All BH2 series observation tubes have constant tube length adjustment. This feature automatically maintains the optimum observation conditions when interpupillary distance is varied, without requiring troublesome adjustments.

B H N W Sup.r Wkkfleld Attachment The BH2SW attachment presents the observ- er with a field of view about twice the size of that obtained with wdinarv obwrvatbn i tubes, and thus reduces to a minimum the need to move the specimen around. This I resutts in a sinnificant Increase in work 1 efficiency. Ths 3-step light path selector and constant tube length adjustment allow I camera focusing through the binocular section for photomicrography. Inclination: 30' to the horizontal Interpupillary distance: 56-75mm (with

I constant tube length adjustment) Maximum F.N.: 26.5

BHBB130 Binocular Obswatlon f ibe The BH2-6130 is designed exclusively for visual observation. Inclination: 30' to the horizontal lnterpupillary distance: 53-75mm (with constant tube length adjustment) Maximum F.N.: 21

BHSTR30 Mrmcular Observation Tube This tube increases the efficiency of photo graphic work since it perrnRs camera focusing through the bkocular section. The three-step llgM path selector allows photwnicrography and observation at the same time. Inclination: 30" to the horizontal lnterpupillary distance: 53-75mm (with constant tube length adjustment) Maximum F.N.: 21

BH2.m Wkal Photohrbe This tube presents the most economical way of conducting photomicrography. The bim cular observation tube must be removed in wder to attach the vertical phototube and so ohenration is not pssible at the same time as photomicrography.

Page 11: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

Stages A selection of stages is available, including those suitable for differential interference contrast and fluorescence microscopy.

Specimen Holders

BH2SVR Mechankal Stage with RlgM-Hand Low Drfve Contrds This is the mast popular stage for many areas of research and testing. Covered ball races prevent contamination by dust and glass chips. Scanning area is 76mm O() x 50mm 0. A 270" rotation capability makes this stage highly suitabie for photographic framing and for differential interference contrast purposes. Note: Range of rotation is reduced to 65' when a M e t -

ential interferencecontrast condenser is attached.

BH2WL Mechanical Stege with Left-Hand Low Olhre Controls Has the same features as the BH2-SVR, except that the controls are located on the left. BH2SH Mechanical Stage wlth Horhontal [Mvo Contds The stage controls are horizontally located on the left- and right-hand sides. The stage can be rotated through 40' when the stage control knobs are on the far skJe, and through 180° when on the near side.

BH2SRG Circular Rotatable Stage This is the ideal stage for Nomarski differential interference contrast and polarizdlight micro- scopy. It features a centering mechanism, and graduations at 1 " intervals around the entire circumference. Stage diameter is 142mm. It accepts stage clips or KM and FM attachable mechanical stages.

BH2SVFR Grooved Meshanlcl~l Stage with Right-Hand Law hfve Controls The fluted design prevents slides sticking to the stage surface due to spilled immersion oil, thus eliminating interruptions In micrs scopic work. The X-axis excursion is 52rnm, and Y-axis excursion is 76mm. The stage rotates through 270'.

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BH2-PC Phase Contrast Attachment BH2-KPC Simple Phase Contrast Attachment Phasecontrast is one of the most commonly used techniques in microscopy. It is employed in the examination of living micro-organisms, cells, etc., permlttlng observation of the internal structures of such specimens under differen f degrees of contrast.

BWPC Pham Contnst Attachment *Features an Abbetype turret condenser wtth a numerical aperture of 1.25. Incorporates annular phase plate for lox, 20X, 40X and 100X phase objectives. Incorporates an aperture diaphragm for use in brighMeld. This diaphragm Is automatically disengaged in phasecontrast observation.

*The CT-5 centering telescope with hQh eye point permits slmple and accurate annulus alignment.

*The BH2SW super widefield attachment used in conjunction with S Plan phase- contrast objectives enables observation of super widefield phase contrast images.

*Four types of mtrast-PL, PLL, NH and NM-are available.

Two types of objectives are available for phasecontrast microscopy: S Plan Achre mats and D Achromats. They come in magnifications of 10X 2OX, 40X and 100% and in b t h positive and negative contrast.

BH2-KPC Slmple Phese Contnst Attachment Used in conjunction with a BH2CD brightfield condenser and phase contrast objectives, this attachment presents phase contrast images easily and economically. Available in 1OX 20X and 40X magnifications. I

PL = POSI~VE LOW NH = NEGATIVE HIGH PU= POSITIVE LOW-LOW NM =NEGATIVE MEDIUM

Page 13: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

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Page 14: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BH2-N IC Nomarski Differential Interference Contrast Attachment The Nomarski differential interference technique is used for unstained specimens, such as living cells. Intercellular structure end surface variations can be obsenred as a three dimensional image under various interference colors.

*The turret condenser (NA 1.40) is fitted with Nomarskl brisms for lox, 20X, 40X and lOOX magnifications, as well as light annuli for 1 OX and 40X phase contrast microscopy. These features enabte cornparisms bemen phase contrast and DIC images with the greatest of ease.

aSiandard S Plan A c h m t objecth can be used for DIC microscopy. Super widefield DIC observation is also possible.

.Has an opem aperture for briihtfield chewation.

.The Internate attachment has s magnifi- cation factof of 1 .a.

*IF550 interference filter uGhmv

Page 15: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination
Page 16: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BH2-RFL Reflected Light Fluorescence Attachment The development of new optics makes it possible to use high-energy exciting light, providing brighter fluorescence and improved results.

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.Three levels of exciting light intensity are available: 100%, 25% and 0%.

.An aperture diaphragm allaws ttle precise regulation of exciting light intensity and fluor- image contrast.

*Features a built-in field diaphragm with centering mechanism. This feature elimi- nates stray llgM which c a w flares and reduces image clarity.

*The synchronized exciting filters, dichroic mirrors and barrier fitters all move together, allowing swttch over between two wave h g b at a single touch. F i types of exciting filter sliders are available, each W i n g two exciting fitters. Exciting filters come in five combinatians: U-V, B-G, U-B, B u and UG.

*Additional exciting filters and barrier filters W Y be employed.

mFluorescence attachments using a halogen ligM a r c e are available for use with FlTC staind s~ecirnens which can be examined with a retatlvely small amount of exciting light. Both reflected light and transmitted b light types are available. Btl2-RFLB A reflected l i t fluorescence attachment with halogen tight source, designed for B excitetian. BH2-F ITW These hNo transrnittdQht fluorescence units are designed specifically for B excitation and emplay the l m t t halogen lamp in BHS microscope as their light source. The secondary transmission region at about 630n m in these FtTC filters enables the observation of non-fluorescence areas under red &Meld illumination.

*D Plan Apo UV Series objectives feature an apochromatic design to correct chr~maticabermtkrtscwerawidewavelerigth range. This design guarantees a sharp knage and brlgM Inmination in any excitatian. The new, flwrescencefree i m r s b n oil is also suitable for other d l Immersion ~ u s e d f o r g e n e r a l ~ t i o n . ~ a l s o o f f e r s a l l n e u p o f 4 t y p e s o f fluorescent phase contrast objectives (D Plan Apo/D Apo UVPL Series). When a flwrescent specimen is being scanned, fading of fluorescent light can be pwnted by switoh'hg to phase mtrast ~ . W h e n r e l l e c t e d l ' ~ f i u o r e s c e n c e and transmitted IIgM phase contrast &mmtim are performed simultanewsly, an entire specimen Image, induding the non-fluorescent area, can be obtained.

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Page 17: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BH2-FITC Standard Ouffits iH2-RFL Standard OU

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Page 18: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

Attachments for Group Observation Here is a group of very useful accessories which allow simultaneous viewing by several observers. This is a parficularly convenient feature for discussions between researchers, and is indispensable for teaching purposes.

BH2-MDO MUHI-viewing Attachment .Permits simultaneous observation of the same specimen image in the same orienta- tion by up to five persons.

*The arrow painter can be moved to indicate any area of the specimen. Pointer color (green or orange) can be changed, and brightness adjusted.

Standard Outfits

BH2-00 Dual-viewlng Attachment .The features are exactly the same as the BH2-MDO, except that tl permits observation by two persons.

*There is ample distance between the oberver posklons, allowing relaxed viewing.

*The second observer may manipulate the pointer by means of an additional lever located on the opposite side from the main lever.

Standard Outfits

is used on a BHS System mkrmeape.

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AHSPSW Hlgh Resdutlon Projectlcm Semen Eye strain is minimal even after long periods of viewing thanks to the high resolution presented by this screen. Effective diameter: 155mm Screen image magnification: Objective magnification x NFK photo eyeDiece magnification x 3

AH-MPSW Pmjecth Semen The AH-MPS-W is extremely useful in small group viewing and discussions. Effective diameter: 155mm Screen image magnification: Objective magnification x NFK photo eyepiece magnification x 3

BH-WP Wall Projdon Prkm Used to project an image onto a wall or screen.

Page 20: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

PM-IOADSIPM-10AD Photomicrographic and Cinemicrographic Systems These fully automatic Photomicrographic systems are designed for exclusive use with microscopes. A built-in microcomputer assures easy and failurefree operation. They can also be operated manually, and both accept large format film.

PM-1OAbS Autamatk Pbtmicrographk SY-m .Features both spot (1 %) and Integrated (30%) metering.

mThe built-In mlcrocwnputer autamatically cwnptwates for a film's recipmity failure characteristics and cmtrols expmre time correctly even during lmg exposures.

*An exposure adjustment feature allows the precise control of expmre tlme to match the spclmen's characteristics.

*Incorporates an AE lock mechanism, useful when trimmlng with spot metering or to keep density unlform in wntlnuws pmorarnic photography. Manual exposure control is also available.

.Accepts the following types of film: 3Smm; 4" x 5" sheet; and 3'k" x 4lk" Polaroid.

*The multiple exposure mechanism enables a number of images to be recorded on the same frame of film.

PM-IOAD Aul~metlc Photoml~r~grephk w-n Has the same features as the PM-1MS except that it provides only 60% metering with 35mm film. Note: This system accepts practically dl the accas-

sorb avellable for the PM-tOADS.

PMCTR Cobr Tompatun Modub The PMCTR measures color temperatures from 2,500K to 10,000K. It provides greater accuracy In color temperature readings. PM-IV Ttms L a p Control Unlt Used in conjunction with the PM-1OADS or PM-I OAD, this unit automatically controls the intervals in timelapee photography. Intervals can be set f m one second to 99.9 hours. Permits a total of 9999 frames to be taken.

PM-VSB BrlgMhame M l n r k r Thls viewNnder Is optionally available for the Olympus Photomicrographic Systems PM-1OADS and PM-1 W. Focusing on the double crosslines and the film format frames when photographing dark specimens against a dark background is greatly facilitated by the fact that the frame reticle is illuminated and the color of the frame can be varied from black to red or yellm.

Page 21: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination
Page 22: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

BHSKP Sknple PolaWng Attachment This attachment enables polarized-light microscopy using ordinary LB objectives. A tint plate and other compensators can be used with it. Standard Outffts

BH*POL Palarfdn~ Flht Set Consisting of a palarlzer and an analyzer, this accessory is usefull for detecting birefringent specimen details. B H M A MagnHkatkn Changer Magnification can be set at three levels: 1 X, 1.2% and 1.5X. The phasecontrast annuli can be aligned with the bullt-In Bertrand lens. BH2-DA Drawing Attachment Very useful in the accurate sketching of a magnified specimen image seen through the microscope. Adjustable magnficatbn. B B F H =gout Fllter Hdckr Holds three filters at one time. Rtters can be exchanged at the touch of a finger, resulting in more efficient observation and photomicrography.

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Page 23: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

1. Candidiosls (UuOdenurn, Grooott stain). SPlanP(, NFK2.5XLD 2 Alkaline phosphatase (liver). SPlanlOX, NFK3.3XL.D 3. Acld phosphatase (spleen of Gaucher's disease). SPlanlOX, NFK3.3XLD 4. Nlcotin amkie adenine dinucleatide phosphate dehyclrqlmase (cell line derived

from human malignant melanoma-SEKI). SPlen40X, NFK3.3XL.D

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liaaram of the BHS. BHT and PM-IOAD. PM-IOADS

Page 25: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

Svstem

Page 26: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

L R nhjective Series for Biological Use

S Plan Fluorite Obiedvea

D Plan Achromatic Objectives

D Achromatic Objectives

Phase Contrast Objectives 7 s 1a.a aw.

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Denote: S.W. = Su r WIdefiM. PL = Podth Low Contmst. PLL- Positive LowLow Contrast. NH = Negatlve Hlgh Contrast. NM = Negatlva Medium Contrast. D.1.C. = &8lskl DIff~liltIth! hiWfBliMC9 c0ntf8~t.

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Page 27: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

LB Eyepiece Series

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Page 28: Olympus BHS/BHT System Microscopes (BH-2) brochure · PDF fileBHS System Microscope This top quality microscope features a long-life 12V, I# W halogen lamp which provides bright illumination

It takes a tremendous amount of skills to build a reputation as an innovator among industries as diverse as communications, medicine, information and science Yet that's exactly what Olympus has accomplished since its inception in 1919. Our varied product list is filled with technological achievements and resounding successes. Not only in cameras, but also in a wide range of Microscopes. Fiberscopes. Microcassette recorders. Clinical analysis equipment. Video equipment And more breakthroughs are on the way, particularly in the exciting new field of optt~lectronics, which combines the resources of optics, electronics and precision engineering. At Olympus, we've earned our reputation with an unfailing commitment to heavy research and development With an uncompromising dedication to quality, precision and accuracy. And with a stubborn unwillingness to follow the crowd. That's why we'll continue to lead the way with original products that surprise you, assist you, involve you, and fulfill you.

1 Photographic. Medical, Microscopic, Industrial & Business Equipment

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