Odournet_SCENT SIGNATURES AND HOW TO MEASURE THEIR PERFORMANCE_ SPC2014
-
Upload
nick-jones -
Category
Documents
-
view
68 -
download
0
Transcript of Odournet_SCENT SIGNATURES AND HOW TO MEASURE THEIR PERFORMANCE_ SPC2014
42 SPC September 2014
ODOURNET
The monitoring of the scent-
signature released from
cosmetic products is a
complex task. Dr. Hansruedi
Gygax gives an overview of
state of the art sensory and
analytical approaches.
The Odournet Group, the
largest team of experts in
consultancy for environmental
odour assessments and
olfactory product and material
testing has the most complete
capacities for providing
services focused on sensory
and analytical odour
measurement techniques and
also combinations thereof.
Consumers pay more and
more attention to scents, and
to a healthy environment.
Often the presence of
malodours is perceived as
compromised hygiene or even
as health threats. The
increasing use of highly
functional cosmetic products
reflects how much consumers
care about their health, their
appearance, and they also
request longer-lasting
protection against body odour
formation, which has been
recently described by D.
McCamlie in his article about
Malodour Management in the
context of sweat formation.
A choice of standardized
testing options:
There are different possibilities
to measure the performance of
the scent-signature of a
consumer product. Also, in the
case of deodorants and
antiperspirants, there are
methods to monitor the build-
up of malodour such as sweaty
notes over time. It is very
important to select the method
carefully in order to provide
meaningful answers to the
product developers. The odour
measurements can be targeted
to monitor scent-signature
stability over the products
shelf-life or measurements to
support and document product
claims. In many cases, it is
important that the testing
environment is as close as
possible to the real consumer
behaviour and product usage.
Another well-known
phenomenon is the formation
of malodours, which can for
example be due to impurities
in raw materials. For all
sensory odour measurements,
it is very important to have
appropriate screening and
training protocols in place.
Analytical vs. Sensory
Measurements
When performing odour
related measurements, it is
very important to understand
fundamentals about the human
sense of smell as well to know
the detection capacities of the
chosen analytical detector. A
characteristic measure is the
odour threshold concentration,
which by definition is the
concentration where an odour
can be detected with a
probability of 50%. Public
domain information about
sensory threshold
concentrations indicates a
large variability. Very often
malodour key components
have threshold concentrations
far below nanogramm per litre
air. Most of state of the art
analytical detectors at such
concentration levels will only
measure noise. This fact
underlines the importance of
including the human nose as
detector.
Dynamic Olfactometry –
Determination of Odour
Units and Odour Threshold
concentrations.
For measuring odour threshold
concentration, the method of
olfactometry is used
worldwide. An olfactometer
represents a measurement
system which dilutes odour
samples with neutral air and
presents them to a test panel
for assessment. Components
influencing the final panel
results are known and divided
into the three categories: the
olfactometer measurement
system (dilution system), the
assessors and the odour
laboratory. To standardize such
measurements the guidelines
and norms of EN 13725 are to
be applied.
The odour concentration is
often expressed in the so-
called odour units (the
measured dilution facture until
SCENT-SIGNATURESAND HOW TOMEASURE THEIRPERFORMANCE
Figure 2: Olfactometer TO8 by Odournet
Figure 1: Threshold measurements, here a representation of data published by Nagata (2003), show the
immense span from less than 10-6 ppm up to threshold values of over 1000 ppm (a span of 1:10’000’000’000).
Usual analytical detectors would demonstrate a dynamic sensitivity range of 1:10’000. Commercial FID/PID
and e-nose type of sensors reach their sensitivity limits below 0.1 to 0.01 ppm, more sensitivity is reached
with quadrupole MS detectors. GC-MS TOF reaches the lowest sensitivity. The figure demonstrates that a
separation technique (like GC) combined with the human nose as detector is able to go below state-of the art
analytical detection limits. Figure prepared using a graph by Dr. Peter Boeker, University of Bonn.
Ordournet_supplement_Layout 1 27/08/2014 16:41 Page 34
September 2014 SPC 43
ODOURNET
the threshold is reached). The
parameters are useful to
describe the odour impact of
raw materials in a mixture and
also to study the longevity of a
scent-signature during its
application on skin. Odour
units can not directly be linked
to perceived intensity. For
such measurements different
sensory methodologies have
been developed.
Odour intensity and hedonic
tone tests
Opening a product-container
or the application of the
product onto skin releases the
volatile compounds, which can
often be perceived instantly. To
assess the odour in a
reproducible way, the most
common parameters
measured are intensity and
hedonic tone. It is a
challenging task to determine
these on an absolute scale
since intensity and hedonic
tone cannot be judged
independently. Humans tend
to underrate the odour
intensity when it is pleasant
and would overrate it when it
is a malodour related stimulus.
The standardization of such
measurements is very
important. The VDI 3882
allows the rating of intensity
on a 7 point scale by
answering the question “How
strong is the odour?”, while
the measurement of hedonic
tone, which can be performed
at the same time, answers the
question of “How pleasant is
the odour?”. Depending on the
information required, this can
be done with trained expert
panels or with representative
panels.
Beside intensity and hedonic
tone, it is also possible to
record odour acceptance or to
focus on the odour character.
One of the most demanding
tasks is to establish a sensory
description of the odour
character of a scent-signature.
It requires extensive training of
expert panels prior to do a
sensory odour description.
Naïve panellists, however, can
do a so-called polarity profile,
which uses pairs of adjectives
to describe an odour.
As a very important aspect of
sensory odour measurements,
it is necessary to highlight the
need of consistent smelling.
Even evaluation from the
smelling strip has to be
learned and trained. To
facilitate consistent smelling
Odournet has developed the
PureSniff device: This is an
instrument which presents the
headspace over a sample in
undiluted form when a
panellist activates a switch. It
is a universal tool for preparing
and presenting the headspace
and thus presents the scent-
signature of a product in its
pure form.
The assessing of scent-
signatures by panellists is very
fast, and allows capturing
sensory related information
based on the human
perception. Nevertheless the
measurement of molecular
based information is
mandatory for revealing
important facts, which
determine the scent-signature
performance over time.
High-End Molecular
Spectroscopy can be linked
to human perception.
Over the last decades,
Figure 3: Odour parameters
Figure 5: PureSniff II by Odournet
Figure 4: Polarity Profile
Ordournet_supplement_Layout 1 27/08/2014 16:41 Page 35
44 SPC September 2014
ODOURNET
Molecular Spectroscopy
methods to analyze headspace
compositions have evolved
tremendously. Today’s GC-
MS/TOF instruments can
detect molecular traces at
concentration levels of a 100
times smaller than standard
GC-MS Instruments. Scent-
signatures, especially when
they contain natural
ingredients, reveal a high
complexity. The combination
with the human nose as
complementary detector,
allows recording the GC-
Olfactometry trace. Linking the
molecular information from
GC-MSTOF with the perceived
intensity and perceived odour
quality, allows detailed
understanding of the key
odour impact molecules
present in the scent-signature.
With this analysis, it is possible
to identify molecules
responsible for scent-signature
distortion.
Innovative Headspace
monitoring by GC-IMS
Very recently an interesting
combination of analytical
technology became available.
While IMS has been used
successfully for fast detection
of airborne molecules, it had
its main application to detect
traces of plastic explosives at
airports, to find traces of
drugs, or for military
applications such as the
detection of warfare agents.
The combination with GC
separation opens up a new
technique to visualize the
presence of scent-signatures
in a 2-D Fingerprint. The
headspace of a product is
collected and injected in a
short GC- multi-capillary
column. When eluting from the
column, the molecules are
ionized, and analyzed in Ion
Mobility Drift-tube. Within a
short sample processing time
(few minutes), the 2D
fingerprint is recorded and can
be analyzed and compared in
various ways. Presence or
absences of key components
of a scent-signature are seen
immediately and sophisticated
data processing methods
allow a classification of
samples, as well as a
quantification of molecules of
interest.
GC-IMS in particular can be
used to observe the 2D
fingerprint over time to study
the dynamic changes of a
cosmetic product or perfume
after its application onto skin,
or to compare the
similarity/difference of
products. And last but not
least, it is possible to visualize
the formation of off-notes
during the product’s shelf-life.
Conclusion
The monitoring of the scent-
signature released from
perfumed products, and in
particular from cosmetic
products, is a complex task.
The described methods can
be individually applied on the
most various test designs,
depending on the question to
be solved. On the other hand,
a high standardization in each
step of the complete process
of odour measurement (e.g.
sample preparation, sample
evaluation, panel selection
and training) is the key for
reliable and repeatable
results. The Odournet Group
has in-depth expertise and a
full range of best in class
analytical and sensory
measurement options to
study the performance of
scent-signatures.
Figure 6: GC-Olfactometry
Figure 7: Heat map visualization of a GC-IMS measurement; © GAS-
Dortmund
Figure 8: PCA analysis based on the results of the GC-IMS: Odour
comparison of a reference (sample 8) with various samples, finding
which samples are very similar to the reference and which are not
Ordournet_supplement_Layout 1 27/08/2014 16:41 Page 36
Ordournet_supplement_Layout 1 27/08/2014 16:41 Page 37