Sample Purge Gas N2. 100ml/min 0.1Mpa

Dry Purge Gas N2. 100ml/min 0.1Mpa

Stirrer

Flow Controller

Teflon Chamber

Trap Sample tube mini-PAT

mini-PAT

Odor Analysis Jam (Different kind of Fruits Jam) (Pyrolyzer JCI-22 + mini-PAT with the following devise to trap odor)

160ml

Quartz Glass Sample Tube ID: 4mm Length: 36mm Absorbent :Tenax GR 25mg Pyrofoil :F280

Curie Point Injector JCI-22

Preparation 1 mini-PAT Blank アバンダンス

3,000,000

How To Bake mini-PAT 1, BO-6 /BO-15 (Baking Oven System) 300℃ for 3 hours / 100ml/min (N2) 2, GC Oven GC 300℃ for 1 hour 3, JCI-22 Blank start 3 times

CO

2

アバンダンス 3,000,000

CO

2

Preparation 2 Contamination in Pure Water 50ml

2Et

hyl

hex

ano

l

* Against each Jam sample 20g, added 50mL pure water before using Stirrer.

GC-2010 Equivalent time 3 min

Oven temperature

Initial 35℃ 5 min Rate-1 3℃/min Final-1 100℃ 0 min Rate-2 8℃/min Final-2 250℃ 10 min

Analysis time 55.42 min Interface temperature 250℃ Column flow rate 1.0ml/min Split ratio 1:10

QP-2010 Ion source temperature

200℃

Mass range 29-500 EM gain 0.95 kv Solvent cut 0 min Scan time 0.2 min ～55 min

Column

DB-WAX Inside diameter 0.25 mm Length 60 m Film thickness 0.25 um

Odor Analysis Mix Jam (Strawberry, Apple, Grape)

CO2

#1

#2

#3 #4

#5

#6 #7

#20

#19 #18

#17

#16

#14

#15

#13

#8

#9

#11

#10

#12

アバンダンス 3,000,000

アバンダンス 1,000,000

#1

#2 #3

#4

#5

#6 #7

#20

#19 #18 #17

#16

#14

#15

#13

#8

#9

#11

#10

#12

Odor Analysis Mix Jam (Strawberry, Apple, Grape)

Enlarged view

Peak No. Ritention time Compound name Odor Kinds

# 1 6.198 Ethyl acetate Fruit flavor

# 2 9.076 Imidazole

# 3 9.387 Butanoic acid, methyl ester Fruit flavor

# 4 11.544 Butanoic acid, ethyl ester Banana Pineapple

# 5 12.265 Butanoic acid,2-methyl-,ethyl ester,(+ -)- Apple

# 6 13.181 Butyl acetate Apple, Banana

# 7 13.508 Hexanal Grass green smell

# 8 15.501 Isoamyl acetate Banana, Melon

# 9 21.009 Hexanoic acid, ethyl ester Apple, Japanese Sake

# 10 22.875 Acetic acid, hexyl ester Apple, Flower smell

# 11 23.575 Octanal Fruit flavor

# 12 24.185 1-Octen-3-one Japanese Sake smell

# 13 25.202 2-Heptenal, (Z)- Aged person smell

# 14 25.738 2-Hexene-1-ol, acetate Grass green smell

# 15 26.885 Hexanol Green beans smell

# 16 29.504 2-Octenal, (E)- Aged person smell

# 17 30.202 Acetic acid Vinegar

# 18 30.469 Furfural Almond

# 19 34.773 2-Decenal, (E)- Aged person smell

# 20 38.276 Geranyl acetone

Peak List

アバンダンス 3,000,000

Acetic acid, octyl ester: Fruity artificial sweetening Linalool: Lily, Lavender smell Nerolidol：Ethyl caproate (Ethyl hexanoate) Apple smell

CO

2

Eth

yl a

ceta

te

Bu

tan

oic

aci

d,

me

thyl

est

er

Bu

tan

oic

aci

d,

eth

yl e

ste

r

Bu

tan

oic

aci

d,2

-me

thyl

-,e

thyl

est

er,

(+ -

)-

He

xan

oic

aci

d,

eth

yl e

ste

r

Lin

alo

ol

Ne

rolid

ol

Ace

tic

acid

, h

exy

l est

er

Ace

tic

acid

, o

ctyl

est

er

2-H

exe

ne

-1-o

l, a

ceta

te

Odor Analysis Strawberry Jam

アバンダンス 100,000,000

β-M

yrce

ne

Lim

on

en

e

γ-Te

rpin

en

e

Myrcene : Fragrance Limonene ：Citrus Fruits Skin γ-Terpinene：Essential oil

Odor Analysis Orange Marmalade Jam

アバンダンス 10,000,000

Ocimene：Fragrance Decanal ： Citrus Fruits, buckwheat Terpineol：Lilac fragrance Neryl acetate：Essential oil Geranyl acetate：Fruit fragrance

Oci

me

ne

2-C

are

ne

De

can

al

Ace

tic

acid

, o

ctyl

est

er

Ne

ryl a

ceta

te

Ge

ran

yl a

ceta

te

Terp

ine

ol

β-M

yrce

ne

Lim

on

en

e

γ-Te

rpin

en

e

Enlarged view Odor Analysis Orange Marmalade Jam

アバンダンス 3,000,000

Limonene ：Citrus Fruits Skin Linalool: Lily, Lavender smell

Lim

on

en

e

CO

2

Tolu

en

e Li

nal

oo

l

2-E

thyl

he

xan

ol

Odor Analysis Blue Berry Jam

アバンダンス 4,000,000

Now we know Hexanal, Hexanol and Geranyl acetone detected in Mix Jam (Strawberry, Apple, Grape) was from Apple Jam.

Lim

on

en

e

Tolu

en

e Fu

rfu

ral

Bu

tyl a

ceta

te

He

xan

al

He

xan

ol

Ace

tic

acid

2-E

thyl

he

xan

ol

Ge

ran

yl a

ceto

ne

CO

2

Odor Analysis Apple Jam

アバンダンス 3,000,000

Linalool: Lily, Lavender smell Limonene ：Citrus Fruits Skin Terpineol, Furfural

Terp

ine

ol

Lim

on

en

e

Tolu

en

e

Furf

ura

l

He

xan

al

Ace

tic

acid

Eth

ano

l, 2

-(2

-eth

oxy

eth

oxy

)-

CO

2

Lin

alo

ol

2-E

thyl

he

xan

ol

Odor Analysis Apricot Jam

アバンダンス 30,000,000

Ocimene isomer: Fragrance, no color

Oci

me

ne

Oci

me

ne

Ocimene isomer

Oci

me

ne

Odor Analysis Mango Jam

アバンダンス 3,000,000

Oci

me

ne

He

xan

al

β-M

yrce

ne

Oci

me

ne

Bu

tan

oic

aci

d,

eth

yl e

ste

r

Iso

amyl

bu

tyla

te

3-C

are

ne

Lim

on

en

e

Oci

me

ne

Furf

ura

l

2-E

thyl

he

xan

ol

Eth

ano

l, 2

-(2

-eth

oxy

eth

oxy

)-

Odor Analysis Mango Jam

Enlarged view

アバンダンス 3,000,000

2-Pentylfuran : Organic synthesized material, fruit fragrance, sweet.

Lim

on

en

e

Tolu

en

e

2-P

en

tylf

ura

n

No

nan

ol

CO

2

2-E

thyl

he

xan

ol

Odor Analysis Marron Clean

Summary

Sample Detected compounds

Mix Jam Butyrate groups, Acetate groups, aldehyde genera

Strawberry Jam Acetic acid, octyl ester, Linalool, Nerolidol

Orange Marmalade Myrcene, Limonene, γ-Terpinene, Ocimene Decanal, Terpineol, Neryl acetate, Geranyl acetate

Blueberry Jam Limonene, Linalool

Apple Jam Ethyl acetate, Limonene, Hexanal, Hexanol, Geranyl acetone

Apricot Jam Linalool, Limonene, Terpineol, Furfural

Mango Jan Ocimene isomer, Isoamyl butylate, Ethyl butyrate, 3-Carene, Myrcene, Limonene, Furfural

Marron Cleam 2-Pentylfuran, Limonene, Nonanol