Nucleic Acids 101. Last week’s grand challenge: Reading and interpreting genetic...

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Transcript of Nucleic Acids 101. Last week’s grand challenge: Reading and interpreting genetic...

  • Slide 1
  • Nucleic Acids 101
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  • Last weeks grand challenge: Reading and interpreting genetic information
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  • DNA Repair: DNA polymerase 10 -7 Mismatch repair 10 -9
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  • Genomics
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  • Restriction Nucleases
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  • Ligation
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  • Grand challenge: Every disease requires a tremendous amount of interdisciplinary effort to understand and fight it. Can this be streamlined?
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  • The frequency of crossing-over is ~proportional to the physical distance between them. Find DNA markers close to and flanking the gene of interest
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  • Positional Cloning A chromosome has a single DNA molecule running the length of the chromosome Map the gene for the trait in question in that DNA molecule; identify close flanking markers Walk between the flanking markers Identify the gene in question between the markers
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  • How do you map a gene? Collect DNA samples from family members in a pedigree Seek linkage to a polymorphic DNA marker Take advantage of the Human Genome Project to identify the chromosomal region Narrow down the location in the overlapping clones
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  • What are DNA markers? RFLP--Restriction Fragment Length Polyhmorphism SNP--Single Nucleotide Polymorphisms
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  • Mutation destroys a restriction site, causing a RFLP (This is also a SNP--Single Nucleotide Polymorphism)
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  • RFLP
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  • STRP or VNTR
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  • Pedigree of Huntington Disease
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  • SNPs: Single Nucleotide Polymorphisms
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  • NEXT CLASS Central Dogma