*Independent analysis commissioned by Leica Biosystems and conducted by NordiQC according to the manufacturers’ instructions for use and on the corresponding staining platform.

Novocastra HD - DermatopathologyHighly Definitive Antibodies

BOND 7 mL PA0007

Liquid 1 mL NCL-L-CD117-032

Human Gastrointestinal Stromal TumorIntense membrane staining of tumor cells.

CD117 (c-kit Oncoprotein)

Clone EP10

T-Cell Lymphoma, Mycosis FungoidesA skin biopsy, extensively infiltrated by abnormal T lymphocytes which are strongly CD3 positive.

CD3 Clone LN10

BOND 7 mL PA0553

BOND 30 mL PA0122

Liquid 1 mL NCL-L-CD3-565

Human LymphomaMembrane staining of endothelial cells.

CD31 Clone JC70A

BOND 7 mL PA0414

Liquid 1 mL NCL-L-CD31-607

Dermatofibrosarcoma ProtuberansVascular endothelium and dermal spindle cells, within ramified fat, staining with CD34. Lymphoid cells are negative.

CD34 Clone QBEnd/10

BOND 7 mL PA0212

BOND 30 mL PA0354

Liquid 1 mL NCL-L-END

Human Skin, XanthogranulomaSheets of histiocytes showing variable staining. Larger xanthomatous cells are strongly positive.

CD68 Clone 514H12

BOND 7 mL PA0273

Liquid 1 mL NCL-L-CD68

Human Nipple, Paget’s DiseaseMalignant cells show strong cytoplasmic staining. There is no staining in the squamous epithelial cells.

Cytokeratin 7 Clone RN7

BOND 7 mL PA0942

BOND 30 mL PA0138

Liquid 1 mL NCL-L-CK7-560

Trust the HD Difference – Highly Definitive Antibodies for Diagnostic Confidence• Independently benchmarked against leading equivalents by external QA*

• Establishing a new quality standard in IHC; simplifying antibody selection

Novocastra HD - Dermatopathology

Human Skin, Merkel Cell CarcinomaMalignant Merkel cells show moderate to strong cytoplasmic staining.

Cytokeratin 20 Clone Ks20.8

BOND 7 mL PA0022

BOND 30 mL PA0037

Liquid 0.5 mL NCL-L-CK20

Liquid 1 mL NCL-L-CK20

Human Skin, Kaposi’s SarcomaNeoplastic cells show moderate cytoplasmic staining, while normal endothelial cells show strong staining.

Factor VIII related antigen (von Willebrand Factor)

Clone 36B11

BOND 7 mL PA0055 NEW

Liquid 1 mL NCL-L-vWF

Immunoglobulin A Clone N1CLA

Liquid 1 mL NCL-L-IgA

Human AppendixIntense staining of plasma cells and secreted immunoglobulin A.

Human TonsilMembrane staining of peripheral follicular B cells.

Immunoglobulin D Clone DRN1C

Liquid 1 mL NCL-L-IgD

Immunoglobulin G Clone RWP49 Immunoglobulin M Clone

8HC

BOND 7 mL PA0905 NEW

Liquid 1 mL NCL-L-IgG

BOND 7 mL PA0278 NEW

Liquid 1 mL NCL-L-IgM NEW

Human TonsilCytoplasmic staining of the plasma myeloma cells.

Normal Human LarynxIntense membrane staining of lymphocytes.

Human TonsilIntense cytoplasmic and membrane staining of plasma cells.

Kappa Light Chain Clone CH15

Liquid 1 mL NCL-L-KAP-581

Human Skin, MelanomaDispersed cycling neoplastic cells show a distinct nuclear staining reaction.

Ki67 Clone MM1

BOND 7 mL PA0118

BOND 30 mL PA0410

Liquid 1 mL NCL-L-Ki67-MM1

Human TonsilIntense cytoplasmic and membrane staining of plasma cells.

Human Melanoma A melanoma showing cytoplasmic staining of tumor cells.

Lambda Light Chain Clone SHL53

Melan A Clone A103

BOND 7 mL PA0233

BOND 30 mL PA0044

Liquid 1 mL NCL-L-MelanA

Liquid 1 mL NCL-L-LAM-578

Human Skin, MelanomaA melanoma showing strong cytoplasmic staining.

Melanoma Marker (HMB45)

Clone HMB45

BOND 7 mL PA0027

BOND 30 mL PA0625

Liquid 1 mL NCL-L-HMB45

Human Face, MelanomaNuclear and cytoplasmic staining of malignant melanocytes as well as some neural elements.

BOND 7 mL PA0900 NEW

Liquid 1 mL NCL-L-S100p

S100 Clone Polyclonal

LeicaBiosystems.com

Novocastra HDHighly Definitive Antibodies

Benefits of BOND Ready-to-Use antibodies

✓ Optimized for staining performance on BOND

✓ Validated antibody concentrations

✓ Ease of use: just plug and play

✓ Increase standardization through automation

✓ Eliminate variability in antibody preparation

Benefits of concentrated liquid antibodies

✓ Flexibility of use on various platforms1

✓ Adaptable to a variety of detection systems1

✓ Adjustable concentration to suit user’s tissue1

✓ Economical option that retains confidence in clone selection

1. The performance of antibodies should be validated when used with automated platforms or manual staining systems other than stated on Instructions for Use.

Improve workflow efficiency and streamline validation.

95.11738 Rev F · 03/2016 · Copyright©2016 Leica Biosystems Newcastle Ltd. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Novocastra and BOND are trademarks of Leica Biosystems and its affiliates. Other logos, product and/or company names might be trademarks of their respective owners. The EP clones have been created by Epitomics Inc., using Epitomics’ proprietary rabbit monoclonal antibody technology covered under Patent No.’s 5, 675, 063 and 7, 402, 409.

Independently Evaluated* Specifically SelectedEach antibody in the range has been independently evaluated* by external QA, NordiQC, in comparison with equivalent products from other vendors. The range represents the highest performing antibodies that Leica Biosystems offers for the most commonly performed IHC tests.

*Independent analysis commissioned by Leica Biosystems and conducted by NordiQC according to the manufacturers’ instructions for use and on the corresponding staining platform.