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November 10th, 2016
Julie Webb, IDEXX Laboratories
Committee Chair
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This Webinar IS…
2017 Phase II Clinical Pathology Exam
in Tampa
Website resources
Highlights of Tampa facility
Sample Questions (essays and MC)
Q&A
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This Webinar IS NOT…
Phase I exam
Phase II exam in Ames
Anatomic Pathology exam (Nov 17)
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This Webinar IS…
Being recorded
Webinar recording and powerpoint will be
posted on ACVP website for review
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Website Resources
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Website - Administrative
Administrative
information relevant
to all exams
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Website - Content
Information concerning
content or taking of a
specific exam
(facilities, schedules,
sample questions,
other resources)
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Phase I
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Phase II Ames
2017 is the final year the traditional Phase II examination is
being offered in Ames, IA
Only certain (repeat) candidates are eligible for the
traditional examination in Ames in 2017
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Phase II AmesAmes candidates:
Review reading list –
different cut-off date!
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Phase II Tampa
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Phase II CP Exam in Tampa
One day exam (7.5 hours)
August 17, 2017
ABP Testing Center, Tampa, FL
Computer-based
Very user-friendly
Coming to website: computer details
Short practice test on exam day
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Phase II CP Exam in Tampa
Day divided into 3 sessions
Essays (Glass slides and Biochem cases)
Interpretation MCQs
Knowledge MCQs
All sessions contain a mixture of topics,
conditions and species
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Phase II Clinical Pathology
Day divided into 3 sessions
But all scores are combined into a single
pass/fail result for the entire exam
What score do I need to pass?
New exam = new standard setting study
Will not know cut score (passing point) until
after the exam has been taken
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Phase II Clinical Pathology
Essays
Interpretation MCQs
Knowledge MCQs
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Essays
3 hour session
10 glass slides and 2 biochem essays
~ 12 minutes/glass slide
~ 30 minutes/essay
Approximate glass breakdown
40-50% cytology
30-40% blood smear
10-20% bone marrow
0-10% histopathology
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Essay Logistics
Previous (Ames) = handwritten, page limit
1 page/glass slide
5 pages/biochem essays
Going forward (Tampa) = character limit
8000 characters, including spaces
~ 2 full typed pages in Word
NOT an issue for glass slides
Also should not be an issue for chem essays
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Essay Logistics
Solitary carrel
Will not share slide flat with neighbor
But cases will likely be divided into two
groups (A and B) and each candidate will
exchange group A for group B (or vice
versa) when ready
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Essay Logistics
Microscope provided
But you may bring your own
No timers/watches allowed
“Time remaining” on computer
3:00 --- 0:004, 10, 40 and 100x
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Essay Logistics
Biochem case data - provided on paper
Easier to visualize, scribble notes…
Enter write-ups on computer
Only information entered electronically will be
graded
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Glass Slides
Provided for you
Sample type, signalment, lesion location +/-
other clinical information
You provide (point form or paragraph)
Description
Interpretation (includes additional tests)
Total points per case = 20
Description (10-16)
Interpretation (4-10)
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Glass Slides, Provided to You
Historically (Ames)
Sample Type
Site
Species
Going Forward (Tampa)
Sample Type
Site
Signalment
+/- additional lesion
description or brief
clinical information
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Glass Slides, Provided to You
Historically (Ames)
Blood smear, dog
FNA, liver, cat
Bone marrow core, horse
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Glass Slides, Provided to You
Going Forward (Tampa)
Blood smear, 9-year-old MN Golden
Retriever, presenting for vomiting
FNA of an enlarged, rounded and
hypoechoic liver, 12-year-old FS DSH cat,
presenting for icterus
Blood smear, 22-year-old TB gelding
Biopsy of skin mass, 9-month-old dog
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Glass Slides, Provided to You
What does this change for your write-ups?
Blood smears, cytology, histology:
Description
Nothing
Interpretation
Maybe nothing
May alter a differential list or additional tests
you would recommend
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Bone Marrow, Provided to You
Historically (Ames)
No CBC data or clinical information
Going Forward (Tampa)
Brief summary of relevant CBC data or
clinical information that will tell you why this
bone marrow sample was collected
Chronic non-regenerative anemia
Marked leukocytosis with rare atypical cells
Lytic lesion seen on radiograph
Hypercalcemia work-up
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Bone Marrow, Provided to You
What does this change for your write-ups?
Description
Nothing
Interpretation
Better able to characterize cell responses
(normal, hypoplasia, hyperplasia)
May alter a differential list or additional tests
you would recommend
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Glass Slide Examples
Blood Smear
Bone Marrow
Cytology/Histology
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Blood Smears
Description
– RBCs, WBCs, Platelets – mention all three, even
if normal (density/numbers, cell morphology)
– Extracellular material, inclusions, organisms,
atypical cells…
– If atypical cells or organisms, describe them in
sufficient detail using proper terminology
○ Size, shape, colour, nuclei, nucleoli, identifying
features…
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Blood Smears
Description (cont’d)
Density/number descriptors can be qualitative
(mild, marked) as opposed to quantitative
(PCV ~20%)
WBC descriptors can be qualitative (mostly
segmented neutrophils with fewer…) as
opposed to quantitative (85% neutrophils)
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Blood Smears
Interpretation
RBCs, WBCs, Platelets – mention all three,
even if normal
An interpretation is NOT just a repeat or
summary of your description (see next slide)
Likely causes and/or additional tests (to
confirm your dx, distinguish between ddx)
also go here
Be as definitive and specific as possible
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Blood Smears
NO, not an interpretation
Anemia with marked
polychromasia and
many Heinz bodies
Leukocytosis with
segs, many bands and
toxic change
YES, an interpretation
Regenerative anemia
due to oxidative
damage/hemolysis
(likely causes = …)
Inflammatory
leukogram
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Blood smear from an anorexic 2-year-old cat
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Cat Blood Smear (Description) Points
Background
Basophilic, crescents or proteinaceous 1
RBCs
Decreased 1
No polychromasia 1
Platelets
Decreased 1
WBCs
Marked (1) increase (1) 2
Mostly atypical/immature/neoplastic 1
-Large 1
-Basophilic cytoplasm 1
-Variable nuclei (mention at least two of indented,
bilobed, binucleate, micro/satellite nuclei)
2
-Nucleolus 1
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Cat Blood Smear (Interpretation) Points
Hematopoietic neoplasia 2
-Differentials include acute leukemia (1) and
lymphoma (1)
2
-Cell characterization (flow, ICC or cytochemistry) 1
-FeLV testing 1
Non-regenerative anemia and thrombocytopenia 1
-myelophthisis 1
Note for grading keys:
All grading keys contain flexibility to accommodate the different ways that different
people say the same thing (alternate wording that demonstrates the same
knowledge will be accepted)
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Bone Marrows
Description
Cellularity, particles, iron
Mention all three lines (even if normal)
○ Extent? Maturation? Morphology?
M:E ratio
Lymphocytes, plasma cells, macrophages
Atypical cells, organisms, matrix or
extracellular material
Bony changes, fibrosis (histology)
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Bone Marrows
Interpretation
Mention all three lines and any other
diagnosis/abnormality
Include additional tests if relevant (to confirm
dx, differentiate between ddx…)
Be as definitive and specific as possible
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Bone marrow section, 8-year-old Rottweiler with
persistent moderate thombocytoepnia
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Focal region…
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Dog bone marrow section (Description) Points
Highly cellular 1
Small amount of iron 1
M:E approx 1:2 1
Megakaryocytes
Many 1
All stages present or normal morphology 1
Erythroid (describe at least two)
Complete, orderly/synchronous (mostly mature),
normal morphology/no dysplasia
2
Myeloid (describe at least two)
Complete, orderly/synchronous (mostly segs), normal
morphology/no dysplasia
2
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Dog bone marrow section (Description) Points
Neoplastic cells
-focal region 1
-arranged in sheets 1
-describe at least two of: shape, eosinophilic
cytoplasm, round to oval nucleus, stippled chromatin
1
-marked pleomorphism noted (very large cell or
macronucleus)
1
-binucleated or mitotic figures 1
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Dog Bone Marrow Section (Interpretation) Points
Malignant neoplasm 2
-primary differential = histiocytic sarcoma 2
-confirm with IHC (MHCII, CD18, CD204…) 1
Erythroid, myeloid and megakaryocytic lines
unremarkable (accept meg hyperplasia)
1
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Cytology, Histology
Description
Cellularity, background (cytos)
Location, arrangement (histos)
Cell populations (resident, inflammatory,
neoplastic), organisms, extracellular material
If neoplastic cells or pathologic organism,
describe them in sufficient detail using proper
terminology
○ Size, shape, colour, nuclei, nucleoli, identifying
features…
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Cytology, Histology
Interpretation
Diagnosis (or differential diagnoses)
Additional tests (to refine your dx,
differentiate between ddx…)
Be as definitive/specific as possible
○ Most slides have a definitive diagnosis or very
limited set of reasonable differential diagnoses
○ Listing a long set of unlikely differentials will
not get you the points
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Cat, conjunctival mass, imprints
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Cat conjunctival mass (Description) Points
Highly cellular 1
Small amount blood or stippled eosinophilic
background
1
Epithelium
Clusters or sheets 1
Basophilia, anisocytosis or binucleate 2
Inflammation
Mostly non-degenerate neutrophils 1
Fewer small lymphocytes and macrophages 1
Bi and multinucleated macrophages 1
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Cat conjunctival mass (Description) Points
Organism
Numerous/many 1
In neutrophils and macrophages 1
Yeast 1
Round, oval to cigar-shaped (mention at least two) 1
3-5um 1
Blue to purple cytoplasm, clear halo/wall 1
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Cat conjunctival mass (Interpretation) Points
Pyogranulomatous inflammation 2
Sporothrix (2) schenckii (1) 3
Hyperplastic epithelium 1
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Biochemistry Essays
Provided to you
Signalment, +/- brief history
Case data (CBC, chemistry, +/- UA, +/- other)
You provide (point form or paragraph)
Explanation for the laboratory abnormalities
Summary of the case and ancillary tests
30 points/case
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Biochem Essays – Provided To You
Historically (Ames)
Adult dog
Foal
Going Forward (Tampa)
9-year-old Labrador Retriever presenting for
polyuria and polydipsia
6-year-old cat with history of weakness,
lethargy and possible seizure episode
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Biochem Essays – Provided To You
What does this change for your write-up?
Maybe nothing
May alter a differential list, ranking of
differentials or additional tests you
recommend
May change the significance of a result
(eg. normal Cl in vomiting dog)
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Biochemistry Essays
Approach to a case
Identify abnormalities and group where
appropriate (eg. urea and creatinine, proteins…)
For each abnormality (or group of abnormalities),
provide an explanation, or a limited set of likely
explanations, for the abnormality in this patient
○ Do not list all possible/general causes
○ An “explanation” may be a specific disease
(Addison’s) or a general mechanism (vomiting)
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Biochemistry Essays – Approach
Thrombocytosis
No, do not list all possible
reasons
Physiologic
Reactive/inflammatory
Recent splenectomy
Reactive/inflammatory
Cushings
Iron deficiency
Essential
thrombocythemia
Yes, provide the likely
reason(s)
Reactive/inflammatory
Cushings
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Biochemistry Essays – Approach
Titrational Metabolic Acidosis
No, do not list all possible
reasons
Ketoacids
Uremic acids
Lactic acid
Ethylene glycol
Yes, provide the likely
reason(s)
Uremic acids
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Biochemistry Essays
Approach to a case (cont’d)
All abnormalities should be addressed
Some normal values may also be important
(normal creatinine with high urea)
No right or wrong order to write-up your case
○ CBC – chem – UA – other
○ Major findings – minor findings
After addressing abnormalities, summarize
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Biochemistry Essays
Summary
Overall interpretation
Diagnosis(es) for the patient
May include specific diseases (Lepto) and
more generic entities (nephrotic syndrome)
May be some overlap between your
summary and your earlier explanations
Ancillary tests (to confirm, refine or further
investigate) should be provided
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11-year-old Shetland Sheepdog presenting
for dry haircoat and weightloss.
Hematology (Units) Patient Flag Reference Interval
Hematocrit (%) 47 39 – 57
Hemoglobin (g/dL) 16.3 14 – 21
RBC (x 106/µL) 6.7 5.6 – 8.4
MCV (fL) 70 61 – 73
MCHC (g/dL) 35 34 – 38
RBC Morphology Within normal limits
WBC (x 103/µL) 14.7 H 5.0 – 14.0
Segmented neutrophils (x 103/µL) 13.0 H 2.6 – 10.0
Band neutrophils (x 103/µL) 0.3 H 0.0 – 0.2
Lymphocytes (x 103/µL) 0.7 0.7 – 4.3
Monocytes (x 103/µL) 0.6 0.1 – 0.9
Eosinophils (x 103/µL) 0.1 0.1 – 1.7
WBC Morphology Few Dohle bodies
Platelets (x 103/µL) 626 H 175 – 500
Plasma protein (g/dL) 8.2 H 5.6 –8.0
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Serum Biochemical Profile Patient FlagReference
Interval
Na (mmol/L) 148 141 - 150
K (mmol/L) 4.1 3.9 – 5.3
Cl (mmol/L) 116 109 - 119
Total CO2 (mmol/L) 5 L 19 – 30
Anion Gap (mmol/L) 30 H 15 – 25
Calcium (mg/dL) 10.8 9.7 – 12.3
Phosphorus (mg/dL) 15.0 H 2.2 – 7.9
Urea (mg/dL) 110 H 7.0 - 32
Creatinine (mg/dL) 4.6 H 0.5 – 1.5
Glucose (mg/dL) 130 67 - 132
Total Protein (g/dL) 7.5 H 4.8 – 6.9
Albumin (g/dL) 3.5 2.3 – 3.9
Globulin (g/dL) 4.0 H 2.2 – 3.5
CK (U/L) 120 22 - 491
AST (U/L) 49 21 - 53
ALT (U/L) 443 H 14 – 87
ALP (U/L) 1486 H 20 – 157
GGT (U/L ) 33 H 5 – 16
Total Bilirubin (mg/dL ) 0.3 0.1 – 0.8
Cholesterol (mg/dL) >650 H 149 - 319
Triglycerides (mg/dL) 131 32 - 190
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Urinalysis Patient
Collection Method Cystocentesis
Color Colorless
Clarity Clear
Specific gravity 1.010
Reagent Strip
pH 6.0
Protein 3+
Glucose Negative
Ketones Negative
Bilirubin Negative
Blood Negative
Sediment Exam
RBC (per hpf) Rare (<1)
WBC (per hpf) 1-5
Epithelial cells Few squamous and transitional
Casts None
Crystals None
Bacteria Many cocci
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Thromboelastography Patient Flag Reference Interval
R (min) 5.8 3 – 9
K (min) 1.4 1 – 6
α (degrees) 70.5 34 – 74
MA (mm) 75.5 H 40 – 70
G (d/sec) 15.4 H 3.2 – 10.1
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Adult Dog Chemistry Points
Inflammatory leukogram 1
Thrombocytosis
- reactive/inflammatory 1
- Cushings/hyperadrenocorticism 1
Metabolic acidosis 1
- titrational 1
- uremic acids 1
Renal azotemia 1
Inflammatory hyperglobulinemia 1
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Adult Dog Chemistry Points
ALKP, ALT and GGT
- Steroid or Cushings most likely 1
- Other hepatic/post hepatic cause 1
Cholesterol
- Cushings 1
- cholestasis 1
- protein losing nephropathy 1
Glomerular proteinuria 1
Urinary tract infection 1
- Lower UTI (bladder) vs 1
- Pyelonephritis 1
Hypercoagulable (by TEG) 1
-Cushings 1
-PLN 1
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Adult Dog Chemistry - Summary Points
Renal disease
-Insufficiency 1
-Infection 1
-PLN/Glomerular Disease 1
- Urine culture 1
- UPC ratio 1
- Other testing (AUS, renal FNA, Lepto, BP…) 2
Likely/suspect Cushings 1
- LDDS or ACTH stim 2
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Phase II Clinical Pathology
Essays
Interpretation MCQs
Knowledge MCQs
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Multiple Choice Questions
(Interpretation and Knowledge)
Simple-type MCQs
– One best answer
– Variable # of foils (A-C, A-D, A-E)
Not tricks
– Thorough screening process before and
after exam
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Interpretation MCQs
100 MCQs, 2.5 hours
Image or data-based questions asking
you to interpret the findings
Blood smear, bone marrow, TEG,
cytograms, EM, cytology, histology,
special stains, immunostain stains, flow
cytometry, urine sediment…
Hematology, biochemistry and other
data…
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Blood smear from a cat
Which is the most likely cause of the red cell abnormalities?
A: Disseminated intravascular coagulation
B: Hypertrophic cardiomyopathy
C: Diabetic ketoacidosis
D: Hemangiosarcoma
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Cytograms from an Advia 120. What is the indicated by the circled regions?
A: Lipemia
B: Hemolysis
C: Monocytes
D: Platelet clumps
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Cutaneous mass aspirate from a dog
What color does the indicated structure stain with Masson’s Trichrome?
A: Red
B: Blue
C: Purple
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Nasal flush from a dog
What is the significance
of this specimen?
A: Pollen inhalation
B: Eucoleus infection
C: Rhinosporidium infection
D: Oropharyngeal contamination
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Cytology and histology specimens from an 8cm intra-abdominal mass in a dog
Choose the best diagnosis:
A: Seminoma
B: Lymphoma
C: Histiocytic sarcoma
D: Metastatic carcinoma
E: Transmissible venereal tumor
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R. I. Patient
(Baseline)
Patient
(3 hr H2O dep)
Patient
(vasopressin)
Sodium 134-152 159 159 -
Glucose 6-9 13 - -
USG 1.005 – 1.020 1.003 1.003 1.020
Plasma
Osmolality
299-313 327 340 312
Laboratory results from an African Grey parrot
Results are most consistent with:
A: Diabetes mellitus
B: Medullary washout
C: Psychogenic polydipsia
D: Central diabetes insipidus
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A 5-year-old castrated male golden retriever dog is presented
for lethargy, weight loss, diarrhea and regurgitation.
Parameter (Units) Result Flag Reference Interval
HCT (%) 17.5 L 37-55
RETIC# (x103/uL) 24.8 0-80
WBC (x103/uL) 52.2 H 6-17
PLT (x103/uL) 96.0 L 200-900
Choose the best diagnosis:
A. Chronic monocytic leukemia
B. Acute lymphoblastic leukemia
C. Chronic lymphocytic leukemia
D. Acute myelomonocytic leukemia
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Knowledge MCQs
100 MCQs, 2 hours
Mostly text-based questions pertaining
to mechanisms and diagnosis of disease
and principles of laboratory medicine
Some image or data-based questions
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In cats, prolonged aPTT and normal PT
without a bleeding tendency occurs with
deficiency of:
A. Factor IX
B. Factor XI
C. Factor VII
D. Factor XII
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In a canine cerebrospinal fluid sample,
the presence of extracellular myelin-like
material is most likely associated with:
A. Necrosis
B. Neoplasia
C. Inflammation
D. Sampling artifact
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What is plotted on the x-axis and y-axis,
respectively, in this Levey-Jennings plot?
A. Time and sensitivity
B. Speed and assay range
C. Precision and accuracy
D. Time and concentration
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Blood smear from a horse
Identify the erythrocyte inclusions
A. Theileria equi
B. Babesia gibsoni
C. Neorickettsiae risticii
D. Anaplasma phagocytophilum
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Interpretation vs Knowledge MCQ?
Doesn’t matter very much
Equally weighted, P/F exam
2.5 vs 2 hours
More important
Is this something an entry-level (minimally
competent) candidate should know?
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Additional Q&A session
ACVP Meeting in New Orleans
Tuesday, 12:15-1:15
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Keep Calm, Read Schalm’s