Northwest Microbiology Conference 2018 · Northwest Microbiology returns! Welcome back to the...
Transcript of Northwest Microbiology Conference 2018 · Northwest Microbiology returns! Welcome back to the...
Northwest Microbiology Conference 2018 University of Salford - Salford Quays Campus 16th March 2018
WELCOME!
Welcome to Northwest Microbiology 2018 Northwest Microbiology returns! Welcome back to the University of Salford’s Salford Quays campus for the first time since 2014. For those new to this conference series, Northwest Micro was setup as a collaboration between Northern Universities with the intention of giving Early Career Researchers - our PhD students and Postdocs - an opportunity to showcase their amazing microbiology research.
This year, we have 120 attendees from across 7 institutions. We hope you’ll use this opportunity to interact with each other - share your research, your ideas, develop new collaborations.
Future Conferences We are keen to make this a regular (annual?) event. Please do give us your feedback, through the online survey that will be circulated by email after the conference. Your suggestions and opinions will serve to improve future conferences.
Sponsors This event is free-of-charge to make it accessible to all students. This would not be possible without the kind support from the Society for Applied Microbiology and the Microbiology Society, and from our industrial sponsors, all of whom have stands in the foyer. Please visit them - you may find they have products that are useful for your projects, and you may even win some prizes!
Social Media We encourage you to share through the usual social media platforms (we suggest the twitter hashtag #NWMicro18. Please be aware that some speakers / poster presenters may not wish for their research to be posted online - these will be clearly marked if so.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
PROGRAMME
08:30 - 09:30 REGISTRATION
09:30 Welcome & Housekeeping
09:45 - 10:30 Session 1: Poster Lightning Talks Chair: Christina Bronowski
1 minute talks from all Poster presenters
10:30 - 11:15 COFFEE BREAK & POSTER VIEWING
Offered Talks 1: Microbial Communities Chair: Chloe James
11:15 - 11:30 The Importance of Method Standardization for Human Microbiota Studies
Anna Pulawska-Czub
11:30 - 11:45 Development of the Chicken Intestinal Microbiome Peter Richards
11:45 - 12:00 Molecular Epidemiology of Tick-Borne Haemoparasites in Nigerian Sheep
Babagana Mohammed Adam
12:00 - 12:15 Impact of maternally derived antibodies and infant microbiota on the immunogenicity of rotavirus (RV) vaccines in African, Indian and European infants. Initial results from the RoVI study
Christina Bronowski
12:15 - 12:30 Low dose Trichuris muris infection impairs host immune control of Streptococcus pneumoniae nasopharyngeal carriage.
Alice Law
12:30 - 14:00 LUNCH
Offered Talks 2: Novel Antimicrobials and Antiparasitics
Chair: Prof. Jay Hinton
14:00 - 14:15 Heparins mimetics inhibits malaria parasite Plasmodium falciparum growth and merozoite invasion of the red blood cells in vitro
Muqdad Hmoud
14:15 - 14:30 Antimicrobial activities of novel Chrysin adducts Nicholas Omonga
14:30 - 14:45 Novel Roles for Skin lipids in Staphylococcal Survival Jo Moran
14:45 - 15:15 COFFEE BREAK & POSTER VIEWING
Offered Talks 3: Microbial Genomics and Transcriptomics
Chair: Ian Goodhead
15:15 - 15:30 Identification of a novel phage infection immunity protein by transcriptomic analysis of a Salmonella prophage
Sian Owen
15:30 - 15:45 Microbial mutation rates have evolved a plastic association with population density across domains of life
Huw Richards
15:45 - 16:00 The role of the RNA chaperone ProQ in the Gram negative bacterium Pasteurella multocida.
Emily Gulliver
16:00 - 16:45 KEYNOTE ADDRESS Prof. Miren Iturriza-Gomara
16:45 - 17:00 Closing Remarks & Prizes
17:00 - 18:30 EVENING RECEPTION
NORTHWEST MICROBIOLOGY CONFERENCE 2018
KEYNOTE ADDRESS Professor Miren Iturriza-GomaraProfessor of VirologyInstitute of Infection and Global Health, University of Liverpool
Enteric vaccines: virus diversity versus population diversity
Rotavirus is the commonest cause of severe diarrhoeal disease in children and a major contributor to child morbidity and mortality worldwide. The global introduction of rotavirus vaccines has had significant impact in reducing this burden. However, despite their impact, currently available rotavirus vaccines, like other enteric vaccines such as polio, are less effective in low-income, high-burden countries than in higher income settings. Limitation of vaccine effectiveness in the very countries which need them most could result in a significant continuing burden of potentially vaccine preventable deaths and disease. Multiple explanations for this disparity have been proposed, including virus diversity and host genetics, maternal antibody, age at vaccination, persistent exposure to enteric pathogens in the environment, altered gut microbiota and malnutrition and micronutrient deficiencies. This talk will summarise some of the data rotavirus disease burden and vaccine impact and current research aimed at understanding the factors that may be responsible for enteric vaccine underperformance in low income countries. Options for improving protection against diarrhoeal disease among children worst affected will also be discussed.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
ABSTRACTS: ORAL PRESENTATIONS Offered Talks 1: Microbial Communities
The Importance of Method Standardization for Human Microbiota StudiesAnna Pulawska-Czub
NIHR Health Protection Research Unit in GI, University of Liverpool
Human intestine contains diverse microbiota with significant role in health and disease. Determining bacterial composition in faecal samples through DNA sequencing is a multistep process where choice of analytical procedures can influence and bias final observations.Systematic approach was taken to study the impact of sample storage conditions [neat or homogenized with stabilization buffer: DNAgard, PSP or EtOH in various temperatures for up to 5months]; choice of DNA extraction method [commercial kits: Stratec, ZYMO RESEARCH, QIAGEN, MP Biomedicals] and amplicon target for next-generation sequencing on MiSeq platform [V4 or V3V4].Results demonstrated that homogenization of faecal samples with selected stabilization buffers contributed to better bacterial community preservation in higher temperatures comparing to those left neat or submerged in EtOH. Bacterial phyla abundances varied across DNA extraction methods with highest proportions of Actinobacteria and Firmicutes recovered with ZYMO and Bacteroidetes, Cyanobacteria and Proteobacteria with QIAamp. Finally, significant differences were observed between abundances of over 20 families when alternative variable regions of 16S rRNA gene were sequenced.These data highlight the importance of consistency of protocols among microbiota studies in order to enable cross-comparability. It also provides useful information to help interpret differences observed between studies.
Development of the Chicken Intestinal Microbiome
Richards, P.; Wigley, P.; Fothergill, J; Bernardeau, MUniversity of Liverpool
The intestinal microbiome is a crucial factor in the development of the intestinal immune system and host metabolism. An understanding of how microbial communities develop under normal circumstances can provide a rational basis for probiotic interventions which bolster early immune maturation and metabolic capabilities. The development and succession of the ileal and caecal microbiome in three breeds of broiler chickens between 0 and 42 days post hatch (d.p.h) was analysed using an Illumina MiSeq run. DNA extracted from luminal samples at 0, 3, 7, 14, 21, 28 and 42 d.p.h and mucosal samples at 14, 21, 28 and 42 d.p.h was submitted for sequencing of the V4 region of the 16S rRNA gene. Our results show that the early intestinal microbiome has poor diversity with one or two taxa contributing the majority of sequences. From 3 d.p.h, microbiome diversity begins to increase with differences between the caecal and ileal microbiomes becoming apparent. Over time the caecal and ileal microbiomes diverge and stabilise by 21 d.p.h and 42 d.p.h respectively. Differences in taxa between the caecum and ileum reveal a large functional diversity between the two compartments defining different roles in host metabolism.Significant differences between the caecal mucus and lumen microbiome were identified. Temporal differences associated with the presence of epithelial associated segmented filamentous bacteria were identified between ileal mucus and luminal samples.
Molecular Epidemiology of Tick-Borne Haemoparasites in Nigerian Sheep
Babagana Mohammed Adam1, Vincenzo Lorusso1,2, , Kevin Bown1, Michiel Wijnveld3, Richard Birtles1
1. University of Salford Tick Infections (USALTI) Group, School of Environment and Life Sciences, University of Salford; 2. Global Research & Medical Division, Vetoquinol, Paris, France;3. Centre for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Austria
Tick-transmitted pathogens (TTPs) impose serious constraints to livestock health and productivity in sub-Saharan Africa, including Nigeria. The aim of this study was to assess the occurrence of TTPs in domestic ruminants from North-Western Nigeria, focusing on the so far more neglected species of sheep, using a sensitive molecular approach. 257 whole blood samples were collected from sheep in Kachia grazing reserve, Kaduna State. Detection of TTPs was conducted by means of PCR-based reverse line blotting, targeting six genera of microorganisms including bacteria and apicomplexan protozoa. 75.1 % of sampled animals were infected, with the great majority (78.2%) being affected by multiple infections. Theileria equi-like was the most prevalent microorganism detected (66.3%), followed by Rickettsia spp. (20.2%), Anaplasma centrale (17.5%), Theileria velifera (12.1%), Theileria spp. (10.8%), Ehrlichia sp. Omatjenne (10.1%), Ehrlichia/Anaplasma spp. (10.1%), Theileria mutans (8.9%), Theileria sp. MSD4 (5.8%), Bartonella spp. (3.1%), Babesia bovis (2.7%), Babesia spp. (1.25%), Babesia caballi (0.45%), Ehrlichia ruminantium (0.4%) and Rickettsia spp. of the “thypus group” (0.4%). Lambs were significantly less infected than juvenile and adult sheep. Numerous TTPs were identified in sheep from NW Nigeria that could cause disease and serious production losses. The detection of several zoonotic agents (i.e. Rickettsia spp. and Bartonella spp.) warrants further characterisation studies to better assess risk for human infections in the area.
Impact of maternally derived antibodies and infant microbiota on the immunogenicity of rotavirus (RV) vaccines in African, Indian and European infants. Initial results from the RoVI study
Christina Bronowski1, Edward Parker2, Ira Praharaj3, Alistair Darby4, Nigel Cunliffe1, Miren Iturriza-Gomara1 & the RoVI consortium.
1. Institute of Infection and Global Health, University of Liverpool, 2. Imperial College London, 3. Christian Medical College, Vellore, 4. Institute of Integrative Biology, University of Liverpool
Rotavirus is the commonest cause of severe gastroenteritis in infants under five years of age worldwide. RV vaccination has led to significant reductions in infant mortality. However, RV vaccine efficacy is significantly lower in low income countries, in the very populations that sustain the highest burden. The reasons for the reduced responses are poorly understood. Evidence suggests that alteration of the infant gut microbiota at the time of vaccination may be associated with poor live oral vaccine take and immunogenicity.The study enrolled birth cohorts in three countries: Malawi and India, infant populations in whom RV vaccine efficacy is poor, and the United Kingdom, where vaccine efficacy is high. Infants were followed from birth up to 16 weeks of age. Blood and breast milk samples are collected to investigate the impact of maternal antibody transfer on seroconversion. Serial stool samples are collected to examine gut microbiota development, early exposure to natural RV infection and RV vaccine virus shedding post immunisation. Gut and systemic inflammation at the time of vaccination are also being assessed. Here we present preliminary microbiota results from all three cohorts. This study will ultimately provide data on how maternally derived immunity and the intestinal microbiota, impact on RV vaccine take and immunogenicity in the infant, providing novel data on the maternal microbiota imprinting of the infant gut microbiota in different populations.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
Low dose Trichuris muris infection impairs host immune control of Streptococcus pneumoniae nasopharyngeal carriage.
A. Law; R. J. Flynn; R. K. Grencis; A. Kadioglu; D. R. Neill
University of Liverpool
Nasopharyngeal colonization by Streptococcus pneumoniae and soil-transmitted helminth (STH) infection are common childhood events in many areas of the developing world. It is likely that a substantial proportion of children worldwide are coinfected with pneumococcus and one or more helminths. Helminths modulate systemic host immunity in such a way that ensures chronic infection, with polarization towards a Th1 or T regulatory response often playing a crucial role. Given the importance of balance between these pathways in maintaining asymptomatic pneumococcal carriage, STH-driven immune perturbation may have a significant impact on carriage and susceptibility to invasive pneumococcal disease (IPD). Using a murine pneumococcal-Trichuris muris coinfection model, we investigated the dynamics of pneumococcal carriage and assessed immune responses during simultaneous STH infection. T. muris infection led to increased nasopharyngeal bacterial loads and enhanced dissemination of pneumococci to the lungs and blood. Higher levels of IFNg and TNFa were observed in the respiratory tissues of co-infected mice in comparison to singly-infected mice, suggesting that T. muris-driven host immune modulation plays a role during co-infection. These data address a previously unrealised developing world health issue and data from this study could eventually inform treatment options to combat IPD as well as enhance our understanding of pneumococcal disease dynamics in the developing world.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
Offered Talks 2: Novel Antimicrobials and Antiparasitics
Heparins mimetics inhibits malaria parasite Plasmodium falciparum growth and merozoite invasion of the red blood cells in vitro
Muqdad Hmoud1, Mark Skidmore2, Edwin Yates3 and Paul Horrocks1
1. Institute of Science and Technology in Medicine, Keele University, Staffordshire, ST5 5BG, UK. 2. School of Life Sciences, Keele University, Staffordshire, ST5 5BG, UK. 3. Institute of Integrative Biology, University of Liverpool, L69 72B
Despite the global effort to decrease mortality and morbidity, malaria still causes 430000 deaths and more than 200 million infection cases according to World Health Organisation report 2017. Plasmodium falciparum is the most lethal malaria parasite among other malaria spp. Antimalarial drug resistance has emerged in many malaria affected areas in the world.There is an urgent need to develop new antimalarial therapeutics to overcome antimalarial resistance and to support current advances in antimalarial drug development. Heparin and other glycosaminoglycans have shown antiplasmodial activity against P.falciparum.This activity seems to be mediated by interfering with merozoite surface proteins (MSPs) and erythrocyte membrane binding proteins (PfEMP) resulting in invasion blocking of the erythrocyte.The antiplasmodial activity of heparin has not been exploited therapeutically due to its high anti-coagulation property. Here we report a novel heparin mimetic Glycosaminoglycans that have in vitro antiplasmodial activity. This activity was assessed and compared with their anti-coagulation property offering a new approach to malaria treatment as an adjunct therapy.
Antimicrobial activities of novel Chrysin adductsNicholas OmongaUniversity of SalfordA series of new 7-O-Chrysin derivatives were synthesised. Seventeen compounds were screened against eight bacteria: Escherichia coli 2513, Pseudomonas aeruginosa 2513, Bacillus cereus 2513, Staphylococcus aureus 25923, MRSA 252, Enterococcus faecalis 2513, Pseudomonas fluorescens 2513 and Klebsiella pneumonia 2513 and one fungus - Candida albicans MTCC 227 using Gentamycin and Fluconazole as standard drugs for antibacterial and antifungal studies. Compounds containing ketone groups (C1), methyl groups (C2) and nitrate groups (C3) indicated bactericidal inhibition against a broad spectrum of bacteria tested. S. aureus, E. coli, B. cereus and E. faecalis being the most susceptible bacteria to inhibition by these compounds. MIC of C1 against these bacteria was 25, 50, 50 and 50 µg / Ml respectively. C2 showed bacterial inhibition to these bacteria at MIC values of 50, 100, 100 and 100 µg / mL respectively. C3 showed bacterial inhibition at MIC values of 12.5, 12.5, 25.0 and 50 µg / mL respectively. MIC values indicated by these compounds were better than those indicated by Chrysin and were comparable to those showed by the standard drug Gentamycin. C1 and C3 also inhibited the growth of the drug-resistant bacteria MRSA at doses of 65 and 100 µg / mL respectively. C1 and C3 also showed superior antifungal activities against the pathogenic fungus Candida albicans at MIC values of 50 µg / mL respectively compared to the standard antifungal drug Fluconazole (MIC 125 µg / mL). 7-O-Bromochrysin and 7-O-Alkylchrysin derivatives of Chrysin showed weak antimicrobial activities (MIC: 125 µg / mL) against all bacteria and fungus tested.
Novel Roles for Skin lipids in Staphylococcal SurvivalMoran J, Xizhang Z, Horsburgh M.University of LiverpoolStaphylococci are commensal colonisers of human skin, but several species are also notable opportunistic pathogens, causing diseases ranging from superficial skin abscesses to life-threatening sepsis and pneumonia. Improving our understanding of the factors that enable staphylococcal colonisation of skin may inform strategies to prevent transmission or identify pathways for the design of new antimicrobials to treat infections caused by multidrug resistant Staphylococcus aureus.Research into skin lipids has previously focused on the minor fraction that were long understood to be antimicrobial and influence survival of staphylococci by diminishing cell surface functions. We have examined several other topical lipids to determine their contribution to survival of staphylococci, including squalene and cholesterol. The abundant skin lipid squalene, present in sebum, was revealed to be antimicrobial to staphylococci under low iron conditions by causing iron starvation. Squalene also reduces expression of the pigment of S. aureus thereby decreasing survival from oxidative stress and antimicrobial peptides. Squalene is a precursor for cholesterol biosynthesis and the latter increases survival of staphylococci from antimicrobial skin lipids such as sapienic and linoleic acids. Transcriptomic analysis reveals that cholesterol alters the staphylococcal response to sapienic acid, changing described pathways in cell wall turnover that could act to maintain cell surface function and limit antimicrobial activity. Together, these new descriptions of skin lipid actions could inform key functions of the host barrier that determine the composition of the human skin microbiome.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
Offered Talks 3: Microbial Genomics and Transcriptomics
Identification of a novel phage infection immunity protein by transcriptomic analysis of a Salmonella prophage
Owen, SV and Hinton JCDInstitute of Integrative Biology, University of Liverpool
Bacterial prophages have long been recognised to carry accessory genes that enhance the fitness of their hosts, known as lysogenic conversion. For example, many important pathogens rely on prophage-encoded virulence genes to cause disease. Genes which are expressed during lysogeny are frequently associated with lysogenic conversion phenotypes, so transcriptomic analysis of the prophage regions of bacterial genomes presents an opportunity to identify novel prophage accessory genes.The transcriptome of the BTP1 prophage of Salmonella Typhimurium revealed several genes of unknown function that were highly expressed in infection-relevant conditions, suggesting they may contribute to the biology of the bacterium. Genetic inactivation of one of the putative accessory genes made the S. Typhimurium susceptible to infection by phage P22. Heterologous expression of the accessory gene in a naive host conferred broad-spectrum phage immunity, making the lysogen resistant to diverse and unrelated phages. Homologs of the accessory gene were identified in the genomes of diverse enteric bacteria, suggesting the accessory gene represents a novel family of phage superinfection immunity proteins.This study shows that transcriptomic analysis of prophage regions can identify and assign function to novel phage accessory genes. More broadly, this study demonstrates the potential of next generation sequencing technology to inspire a renaissance in the study of bacteriophage molecular biology.
Microbial mutation rates have evolved a plastic association with population density across domains of life
Richards, H; Krašovec, R; Gifford, DR; McBain, AJ; and Knight, CG
University of Manchester
How and why the rate of spontaneous genetic mutation varies is a fundamental and enduring evolutionary and biological issue. Across organisms, mutation rates have evolved a negative association with effective population size. Yet, within any particular genotype, mutation rates can vary plastically with the environment. We find a strong negative association between mutation rate and population density in the last 75 years of published literature, comprising hundreds of mutation rates (estimated by phenotypic markers of mutation, fluctuation tests) from all domains of life and viruses. Our empirical tests of this association reveal density associated mutation-rate plasticity (DAMP) is present, but variable, in microbes from all domains of life. DAMP depends upon a protein scavenging the same mutagenic oxidised nucleotide (8-oxo-dGTP) in both domains tested. We find DAMP has evolved even at a fine evolutionary scale with significant variation in DAMP between 66 strains of Escherichia coli, where more closely related strains have greater similarity in their degree of DAMP. Such a widespread plastic association in such disparate organisms with such a highly conserved mechanism suggests that DAMP is pervasive across the tree of life. Since mutation rate, like numerous other critical microbial behaviours, responds to population density suggests that, for the de novo evolution of traits such as antibiotic resistance, social circumstances and evolutionary outcomes are tightly linked.
The role of the RNA chaperone ProQ in the Gram negative bacterium Pasteurella multocida.
Emily Gulliver1, Brandon Sy2, Julia Wong2, Deanna Deveson Lucas1, Ralf B. Schittenhelm3, David Powell4, Jai Tree2, Marina Harper1, John Boyce1
1. Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Victoria, 2. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia, 3. Monash Biomedical Proteomics Facility, Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Victoria, 4. Monash Bioinformatics Platform, Monash University, Clayton, Victoria, Australia.
Hfq is a well-characterized RNA chaperone that is involved in riboregulation in bacteria, a process that involves sRNA/mRNA interactions that alter transcript stability and/or translational efficiency, thereby controlling transcript abundance and protein production. Recently, a second RNA chaperone called ProQ has been shown to play a critical role in stabilizing some sRNA/mRNA interactions. To assess the role of ProQ in the Gram negative bacterium P. multocida, we analyzed the transcriptome of a proQ TargeTron® mutant using RNA-seq. In total, 35 transcripts showed increased expression and 96 showed decreased expression. Predicted sRNAs and tRNAs were highly overrepresented in the differentially regulated genes, with 18 predicted sRNAs (p = 3.76x10-9), four with increased expression and 14 with decreased expression, and 17 tRNAs (p =3.78x10-11), all with increased expression, identified. Direct interactions between ProQ and one sRNA, five tRNAs and, 28 mRNAs involved in the production of ribosomal proteins, was confirmed using UV-crosslinking. The P. multocida ompA mRNA showed decreased production at the 3’ end; UV-crosslinking identified that this region also binds to ProQ. Interestingly, P. multocida ompA mRNA was found in hybrids together with other mRNA species, including hupA, indicating that binding between these RNA species is occurring.
NORTHWEST MICROBIOLOGY CONFERENCE 2018
POSTER PRESENTATIONS Poster FullName Organiza3on Title
1 YasminHilliam UniversityofLiverpool
Increasedtolerancetocontactlensdisinfec7ng
solu7oninclinicalisolatesofPseudomonasaeruginosafromkera77spa7ents
2 LauraWright UniversityofLiverpoolInterac7onsofmicrobeswithP.aeruginosainanar7ficialsputummodel
3 EsraaAldawood UniversityofManchester
Dissec7ngtheregula7onofthethreetandempromotersoftheEscherichiacolikps
geneclusteratchromosomallevel.
4 PoppyStevens UniversityofSalford
Characterisa7onofsandfly(Phlebotomusargen7pes)
microbiotalinkedtoLeishmaniainfec7onstatusacrossBihardistrict,India
5 SophieGlossop UniversityofHuddersfield
Bacterialgrowthcondi7onsaffecttheresponseof
culturedkera7nocytestoPseudomonasaeruginosa
extracellularmedia
6 RocioCanals UniversityofLiverpool
Iden7fica7onofkeytranscriptomicdifferencesbetweenglobalandAfrican
sequencetypesofSalmonellaTyphimurium
7 MohamedEshlak UniversityofSalford
An7microbialac7vityofPolyunsaturatedFaTyAcidsrelatedtoChronicWound
Infec7ons
NORTHWEST MICROBIOLOGY CONFERENCE 2018
8 FlavianeSantosdeSouza TheUniversityofSalford
OccurrenceofDWVvariantsinthes7nglessbeeMeliponasubni7da
popula7onthroughoutNEBrazil
9 CharloTeChong UniversityofLiverpool
Pep7doglycanModifica7onModula7ngResistance,
SurvivalandMetabolismofStaphylococcusaureus.
10 HamzaNHameed KeeleUniversityAn7leishmanialac7vityoftemperateplantsecondary
metabolites
11 ChowdhuryMehediHasan UniversityofLiverpool
Compensatoryevolu7onofthecostsofsingleversusmul7drugresistancein
experimentalAcinetobacterbaylyi
12 HebaBarnawi UniversityofManchesterRegula7onofMetalResistanceOperoninCampylobacterjejuni
13 LizethLacharme UniversityofLiverpool
Theembryonatedchickeneggasanalterna7veinfec7onmodelfor
Salmonella
14 HussainEbrahim UniversityofLiverpoolHormoneinterac7onswithPseudomonasaeruginosaurinarytractinfec7ons
15 OluwapamilerinYangomodou UniversityofSalford
GeneExpressionAnalysisandAn7microbialPoten7alofPlantSeedandNutOils
againstS.aureus
16 TerenceMarkham UniversityofSalfordTheexperimentalevolu7onofS.glossinidiusviaoxida7ve
stress
17 BlancaPerezSepulveda UniversityofLiverpool Sequencingof10,000
Salmonellagenomes
18 EljelaniSalim UniversityofSalford OccurrenceofCryptosporidiuminUKsheep
19 WaiYeeFong UniversityofLiverpoolOp7misingextrac7onofRNA
fromintra-macrophageSalmonella
Poster FullName Organiza3on Title
20 PolNadalJimenez UniversityofLiverpool Genomicanalysisofaninsectendosymbiont
21 JohnNewman UniversityofLiverpool
PhenotypicdiversitywithinPseudomonasaeruginosapopula7onscausingurinary
tractinfec7ons
22 AngharadGreen UniversityofLiverpool
Iden7fica7onofniche-specificvirulencefactorsviaexperimentalevolu7onofStreptococcuspneumoniae
23 SamClark UniversityofManchesterExploringtheac7vity
landscapeofacompletesetofan7microbialpep7des
24 VictoriaRimmer UniversityofManchester
Ar7ficialtearsolu7onincreasesvirulencedeterminantsof
Pseudomonasaeruginosabutatafitnesscost
Poster FullName Organiza3on Title
DELEGATE LIST FirstName Surname Affilia3on Email
Chloe James UniversityofSalford [email protected]
Ian Goodhead UniversityofSalford [email protected]
Poppy Stevens UniversityofSalford
Elli Wright LiverpoolSchoolofTropicalMedicine [email protected]
Heather Allison UniversityofLiverpool [email protected]
Jo Fothergill UniversityofLiverpool [email protected]
Chris7na Bronowski UniversityofLiverpool [email protected]
Daniel Neill UniversityofLiverpool [email protected]
Yasmin Hilliam UniversityofLiverpool [email protected]
Miren Iturriza-Gomara UniversityofLiverpool [email protected]
Jay Hinton UniversityofLiverpool [email protected]
Angharad Green UniversityofLiverpool [email protected]
Caisey Pulford UniversityofLiverpool [email protected]
Laura Wright UniversityofLiverpool [email protected]
Steven Kemp UniversityofLiverpool
Eman Alshehri UniversityofLiverpool [email protected]
John Newman UniversityofLiverpool
Rebecca Shears UniversityofLiverpool
Lauren Gordon UniversityofLiverpool [email protected]
Alexander Predeus UniversityofLiverpool
Hussain Ebrahim UniversityofLiverpool [email protected]
Sarah Withers UniversityofSalford [email protected]
Ian Roberts UniversityofManchester [email protected]
Bushra AlLawa7 UniversityofManchester [email protected]
Joe La7mer UniversityofSalford [email protected]
Alice Law UniversityofLiverpool [email protected]
Rachael Antwis UniversityofSalford [email protected]
stephen mar7n UniversityofSalford [email protected]
James Redfern MMU [email protected]
FirstName
Emma Dearing UniversityofLiverpool [email protected]
ELJELANI SALIM UniversityofSalford [email protected]
Alex TompseT UniversityofSalford [email protected]
Craig Winstanley UniversityofLiverpool [email protected]
monica staniek LancasterUniversity [email protected]
Helen Gavillet MMU [email protected]
Nadin Fathallah LancasterUniversity
Chris vanderGast MMU [email protected]
SAEED ALDOSSARI UniversityofLiverpool [email protected]
Yasmine Kumordzi LancasterUniversity [email protected]
SHAMSUL GULAMALI UniversityofLiverpool [email protected]
Hind Alrajeh UniversityofManchester
Rachel Forsyth UniversityofLiverpool [email protected]
Heba Barnawi UniversityofManchester [email protected]
Reem Barnawi UniversityofManchester
Ayed Alshamamri UniversityofSalford [email protected]
Dennis Linton UniversityofManchester [email protected]
Sian Owen UniversityofLiverpool [email protected]
Blanca PerezSepulveda UniversityofLiverpool [email protected]
WaiYee Fong UniversityofLiverpool [email protected]
MohdZulkifli Salleh UniversityofManchester [email protected]
Xiaojun ZhuNatashaHussain Jafri UniversityofManchester
Evita Mayasari UniversityofManchester [email protected]
Gavin AckersJohnson UniversityofSalford [email protected]
Olayode Olatunji UniversityofManchester [email protected]
Julie Truman Qiagen [email protected]
Terence Markham UniversityofSalford
Sean Goodman UniversityofLiverpool [email protected]
AhmadReza Khan
Surname Affilia3on EmailFirstName
Claire Scantlebury UniversityofLiverpool [email protected]
Victoria Rimmer UniversityofManchester [email protected]
Esraa Aldawood UniversityofManchester [email protected]
Adrian Cazares UniversityofLiverpool
Wendy Figueroa
jessica kevill UniversityofSalford [email protected]
Rocio Canals UniversityofLiverpool [email protected]
Emily Gulliver MonashUniversity [email protected]
Lewis Fisher UniversityofLiverpool [email protected]
Adrian Jervis UniversityofManchester [email protected]
Gregory Bulmer UniversityofManchester [email protected]
Hao Yu
Jiayi Tang
Farah Mughal UniversityofManchester [email protected]
Hasan Chowdhury UniversityofLiverpool [email protected]
Srijan Jindal UniversityofManchester [email protected]
AngeziwaChunga Chirambo UniversityofLiverpool [email protected]
Oliver Standing
Sophie Glossop UniversityofHuddersfield
OluwapamilerinDamola Yangomodou UniversityofSalford [email protected]
Arthur Barnard
Josephine Moran UniversityofLiverpool [email protected]
CharloTe Chong UniversityofLiverpool [email protected]
Mohamed Eshlak UniversityofSalford [email protected]
Chris7an Harrison
Muqdad Hmoud KeeleUniversity [email protected]
Flaviane SantosdeSouza UniversityofSalford
Pol NadalJimenez UniversityofLiverpool
hanshuo lu UniversityofLiverpool [email protected]
Hamza Hameed KeeleUniversity [email protected]
Surname Affilia3on EmailFirstName
Lizeth Lacharme UniversityofLiverpool [email protected]
BabaganaMohammed Adam UniversityofSalford [email protected]
Nicky Morgan UniversityofSalford [email protected]
Sam Clark UniversityofBristol [email protected]
Richard Birtles UniversityofSalford [email protected]
Lauren Hafield MMU [email protected]
Anna Pulawska-Czub UniversityofLiverpool [email protected]
Damian RiveT MMU [email protected]
Howard Foster UniversityofSalford [email protected]
Peter Richards UniversityofSalford [email protected]
Nicholas Omonga UniversityofSalford [email protected]
Andrea Gbobaniyi UniversityofSalford
Mohammed Nagshabandi UniversityofManchestermohammed.nagshabandi@postgrad.manchester.ac.uk
Jodie Barber MMU [email protected]
Geraldine Foster LiverpoolSchoolofTropicalMedicine [email protected]
Jessica Balde
Huw Richards UniversityofManchester [email protected]
sladjana malic MMU [email protected]
Jonathan Butler MMU [email protected]
Elliot WhiTard MMU [email protected]
Natalie Callaghan MMU [email protected]
Grace Crowther MMU [email protected]
Niall Hickey MMU
Steven Parker UniversityofManchester [email protected]
Zachary Gander
Anthony Slate MMU [email protected]
Samina Naseeb UniversityofManchester [email protected]
Joels Wilson-Nieuwenhuis MMU [email protected]
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