Northwest Cyantoxin Results 7-11-19€¦ · 2019-07-11 · Colorado Radiochemistry IN00035 New...

LABORATORY REPORT

This report may not be reproduced, except in full, without written approval from EEA.

If you have any questions concerning this report, please do not hesitate to call us at (800) 332-4345 or (574) 233-4777.

Page 1 of 14

=::: e u ro fi n s I Eaton Analytical

Revision date: 03/14/2019

STATE CERTIFICATION LIST

State Certification State Certification Alabama 40700 Missouri 880 Alaska IN00035 Montana CERT0026 Arizona AZ0432 Nebraska NE-OS-05-04

Arkansas IN00035 Nevada IN00035 California 2920 New Hampshire* 2124 Colorado IN00035 New Jersey* IN598

Colorado Radiochemistry IN00035 New Mexico IN00035 Connecticut PH-0132 New York* 11398 Delaware IN035 North Carolina 18700 Florida* E87775 North Dakota R-035 Georgia 929 Ohio 87775 Hawaii IN035 Oklahoma D9508 Idaho IN00035 Oregon (Primary AB)* 4074

Illinois* 200001 Pennsylvania* 68-00466 Illinois Microbiology 17767 Puerto Rico IN00035

Illinois Radiochemistry IN00035 Rhode Island LAO00343 Indiana Chemistry C-71-01 South Carolina 95005

Indiana Microbiology M-76-07 South Dakota IN00035 Iowa 098 Tennessee TN02973

Kansas* E-10233 Texas* T104704187-18-12 Kentucky 90056 Texas/TCEQ TX207

Louisiana* LA014 Utah* IN00035 Maine IN00035 Vermont VT-8775

Maryland 209 Virginia* 460275 Massachusetts M-IN035 Washington C837

Michigan 9926 West Virginia 9927 C Minnesota* 018-999-338 Wisconsin 999766900 Mississippi IN035 Wyoming IN035

EPA IN00035 *NELAP/TNI Recognized Accreditation Bodies

Page 2 of 14

~:: eu rofi ns I Eaton Analytical

Laboratory Report

Client:

Attn:

Report:

Priority:

Status:

PWS ID:

Hillsborough County Board of County Commissioners

Ed Watson

332 N. Falkenburd Rd.

458552

FL6290388

Standard Written

Final

Tampa, FL 33619

Sample Event Code:

PWS Facility ID:

Sampling Point ID:

00002

0388002

SE3

Lab ID Code: IN00035

110 South Hill StreetSouth Bend, IN 46617Tel: (574) 233-4777Fax: (574) 233-82071 800 332 4345

EEA ID #

Client ID Method CollectedDate / Time

CollectedBy:

ReceivedDate / Time

4353985 19070027-02 545 07/11/19 13:45 Client 07/16/19 10:15

4353986 19070027-02 546 07/11/19 13:45 Client 07/16/19 10:15

Sample Information

Report Summary

Detailed quantitative results are presented on the following pages. The results presented relate only to the samples provided for analysis.

We appreciate the opportunity to provide you with this analysis. If you have any questions concerning this report, please do not hesitate to call Jessie Brasch at (574) 233-4777.

Note: This report may not be reproduced, except in full, without written approval from EEA.

Client Name:

Report #:


458552

Authorized Signature Title Date

07/26/2019

Page 1 of 2

Page 3 of 14

=:~ eurofins I Eaton Analytical

I I

Analytical Services Manager

Client Name: Report #: 458552Hillsborough County Board of County Commissioners

Sampling Point ID: PWS ID: FL62903880388002

UCMR Result Summary

AnalyteID #

Analyte Method MRL† Result Units PreparationDate

AnalyzedDate

EEAID #

3311 Anatoxin-a 545 0.0300 < 0.0300 ug/L 07/17/19 13:51 07/17/19 23:44 4353985

3302 Cylindrospermopsin 545 0.0900 < 0.0900 ug/L 07/17/19 13:51 07/17/19 23:44 4353985

3301 Total Microcystins & Nodularins 546 0.300 < 0.300 ug/L 07/19/19 13:30 07/22/19 08:57 4353986

CVTM Percent CV 546 N/A 1.90 % 07/19/19 13:30 07/22/19 08:57 4353986

† EEA has demonstrated it can achieve these report limits in reagent water, but can not document them in all sample matrices.

Page 2 of 2

Page 4 of 14

II I

~

Lab Definitions

Continuing Calibration Check Standard (CCC) / Continuing Calibration Verification (CCV) / Initial Calibration Verification Standard (ICV) / Initial Performance Check (IPC) - is a standard containing one or more of the target analytes that is prepared from the same standards used to calibrate the instrument. This standard is used to verify the calibration curve at the beginning of each analytical sequence, and may also be analyzed throughout and at the end of the sequence. The concentration of continuing standards may be varied, when prescribed by the reference method, so that the range of the calibration curve is verified on a regular basis. CCL, CCM, and CCH are the CCC standards at low, mid, and high concentration levels, respectively.

Internal Standards (IS) - are pure compounds with properties similar to the analytes of interest, which are added to field samples or extracts, calibration standards, and quality control standards at a known concentration. They are used to measure the relative responses of the analytes of interest and surrogates in the sample, calibration standard or quality control standard.

Laboratory Duplicate (LD) - is a field sample aliquot taken from the same sample container in the laboratory and analyzed separately using identical procedures. Analysis of laboratory duplicates provides a measure of the precision of the laboratory procedures.

Laboratory Fortified Blank (LFB) / Laboratory Control Sample (LCS) - is an aliquot of reagent water to which known concentrations of the analytes of interest are added. The LFB is analyzed exactly the same as the field samples. LFBs are used to determine whether the method is in control. FBL, FBM, and FBH are the LFB samples at low, mid, and high concentration levels, respectively.

Laboratory Method Blank (LMB) / Laboratory Reagent Blank (LRB) - is a sample of reagent water included in the sample batch analyzed in the same way as the associated field samples. The LMB is used to determine if method analytes or other background contamination have been introduced during the preparation or analytical procedure. The LMB is analyzed exactly the same as the field samples.

Laboratory Trip Blank (LTB) / Field Reagent Blank (FRB) - is a sample of laboratory reagent water placed in a sample container in the laboratory and treated as a field sample, including storage, preservation, and all analytical procedures. The FRB/LTB container follows the collection bottles to and from the collection site, but the FRB/LTB is not opened at any time during the trip. The FRB/LTB is primarily a travel blank used to verify that the samples were not contaminated during shipment.

Matrix Spike Duplicate Sample (MSD) / Laboratory Fortified Sample Matrix Duplicate (LFSMD) - is a sample aliquot taken from the same field sample source as the Matrix Spike Sample to which known quantities of the analytes of interest are added in the laboratory. The MSD is analyzed exactly the same as the field samples. Analysis of the MSD provides a measure of the precision of the laboratory procedures in a specific matrix. SDL, SDM, and SDH / LFSMDL, LFSMDM, and LFSMDH are the MSD or LFSMD at low, mid, and high concentration levels, respectively.

Matrix Spike Sample (MS) / Laboratory Fortified Sample Matrix (LFSM) - is a sample aliquot taken from field sample source to which known quantities of the analytes of interest are added in the laboratory. The MS is analyzed exactly the same as the field samples. The purpose is to demonstrate recovery of the analytes from a sample matrix to determine if the specific matrix contributes bias to the analytical results. MSL, MSM, and MSH / LFSML, LFSMM, and LFSMH are the MS or LFSM at low, mid, and high concentration levels, respectively.

Quality Control Standard (QCS) / Second Source Calibration Verification (SSCV) - is a solution containing known concentrations of the analytes of interest prepared from a source different from the source of the calibration standards. The solution is obtained from a second manufacturer or lot if the lot can be demonstrated by the manufacturer as prepared independently from other lots. The QCS sample is analyzed using the same procedures as field samples. The QCS is used as a check on the calibration standards used in the method on a routine basis.

Reporting Limit Check (RLC) / Initial Calibration Check Standard (ICCS) - is a procedural standard that is analyzed each day to evaluate instrument performance at or below the minimum reporting limit (MRL).

Surrogate Standard (SS) / Surrogate Analyte (SUR) - is a pure compound with properties similar to the analytes of interest, which is highly unlikely to be found in any field sample, that is added to the field samples, calibration standards, blanks and quality control standards before sample preparation. The SS is used to evaluate the efficiency of the sample preparation process.

Page 1 of 1

Page 5 of 14

Laboratory Report

Client:

Attn:

Report:

Priority:

Status:

PWS ID:


Ed Watson


458552

FL6290388

Standard Written

Final

Tampa, FL 33619


EEA ID #


CollectedBy:

ReceivedDate / Time

4353984 19070027-02 544 07/11/19 13:45 Client 07/16/19 10:15

Sample Information

Report Summary

Method 544 analysis will be performed only if the associated Screening Microcystin by EPA 546 sample result is >=0.3 ug/L.




07/26/2019

Page 1 of 1

Page 6 of 14



of 14

::,..__ ~ t/)

.j/_ 3&9 795 Subcontracted Chain of Custody L./'5f5S-Z.... ,------- - ------ - ---,

--- II I I II I 111111111111111 Hillsborough County Florida

Environmental Laboratory Customer: Northwest Hillsborough CWS

Public Utilities

Sample ID: Customer:

19070027-01-1 Northwest Hillsborough CWS

Address: 17316 N Dale Mabry Hwy

Collector: Grayson, Justin













PWS#

6290388

9456 E Columbus Dr Tampa, FL 33619

Certification Number: E44104

Phone No. (813) 272-5977 x 43635

Site:

lPWTP-002

Collection Date/Time:

Start: 7/11/2019 9:40:00 AM

End: 7/11/2019 9:40:00 AM

Tille: Environmental Specialist I

6290388 lPWTP- Stan: 7/11/2019 9:40:00 AM 002 End: 7/11/2019 9:40:00 AM


6290388 lPWTP- Start: 7/11/2019 9:40:00 AM 002 End: 7/11/2019 9:40:00 AM

TIiie: Environmental Specialist I

6290366 LPWTP- Stan: 7/11/2019 9:40:00 AM 002 End: 7/11/2019 9:40:00 AM


6290388 lPWTP- Stan: 7/11/2019 9:40:00 AM 002

End: 7/1 1/2019 9:40:00 AM


Work Order: 19070027

Scheduled Sample NW UCMR4-CYN Set Name:

Subcontracted Vendor: Eurofins Eaton Analytical ,..

Project: NW - UCMR4-CYN

Contact: Stanley, John (Chief Environmental Scientist)

Phone: (813) 272-5977 x 43635

Profile#

Sample Information Sample Type:

Drinking Water

(Grab)

Drinking Water

(Grab)

Drinking Water

(Grab)

Drinking Waler

(Grab)

Drinking Waler

(Grab)

Container Type:

Glass 250 ml Amber

Glass 250 ml Amber

Glass 250 ml Amber

Glass 125 mL Amber

Glass 125 ml Amber

Preservative: Analysis Method:

Trizma + 2CI acelamide 544 - Microcystins Subcontracted +AA+ EDTA UCMR List

Trizma + 2CI acetamide +AA+EDTA

Trizma + 2CI acetamide +AA+EDTA

AA + bisulfate

Na2S203

545 - Cylindrospermin and Anatoxin Subcontracted

546 - Total Microcystins Subcontracted

Analytes:

Q.

• 19070027-02-1 Northwest Hillsborough CWS

Address: 12950 Fawnridge Blvd

6290388 NWPWF- Stan: 7111/2019 1 :45:00 PM Drinking Waler 002 End: 7/11/2019 1 :45:00 PM (Grab)

Glass 250 ml Amber Trizma + 2CI acetamide 544 - Microcyslins Subcontracted +AA+ EDTA UCMR Lisi

o . ~cc A';/.J\ Collector: Grayson, Justin TIiie: Environmental Specialist I

19070027 -02-2 Northwest Hillsborough CWS 6290388 NWPWF- Start:

Address: 12950 Fawnridge Blvd 002 End:

Collector: Grayson, Justin TIiie: Environmental Specialist I

9070027-02-3 _.Northwest Hillsborough CWS

Address: 12950 Fawnridge Blvd

6290388 NWPWF- Start: 002 End:

Collector: Grayson, Justin TIiie: Environmental Specialist I

Report Generated: 07/11 /2019 02:50 PM

RP.nnrt Version-:~ 5 2 8 1 Last RevisP.d 5/1~/2019 3 :20 :52 PM

7/11/2019 1 :45:00 PM Drinking Water

7111/2019 1 :45:00 PM (Grab)

7111/20191 :45:00 PM Drinking Water

7111/2019 1:45:00 PM (Grab)

Glass 250 ml Amber

Glass 250 ml Amber

Trizma + 2CI acelamide +AA+EDTA

Trizma + 2CI acetamide +AA+ EDTA

I:;~

of 14

Subcontracted Chain of Custody ,---_------------------, ,------~-----------

(~') ~

Hillsborough County rlorida Public Utilities

Environmental Laboratory



Phone No. (813) 272-5977 x 43635

1111111111111111111111 Work Order: 19070027

Scheduled Sample NW UCMR4-CYN Set Name:

Subcontracted Vendor: Eurofins Eaton Analytical ~

Sample Information

Customer: Northwest Hillsborough CWS

Project: NW - UCMR4-CYN

Contact: Stanley, John (Chief Environmental Scientist)

Phone: (813) 272-5977 x 43635

Profile#

Sample ID: Customer: PWS# Site: Collection Daterrime: Sample Type: Container Type: Preservative: Analysis Method: Analytes: 'Res;aucR chlti t1"1L 4.bttnf 9070027-02-4 Northwest Hillsborough CWS 6290388 NWPWF- Start:

Address: 12950 Fawnridge Blvd 002 End:

Collector: Grayson, Justin Title: Environmental Specialist I

19070027-02-5 Northwest Hillsborough CWS 6290388 NWPWF- Start:

Address: 12950 Fawnridge Blvd 002

End:

Collector: Grayson, Juslin Title: Environmental Specialist I

:,~y ~~ Date/Time. t - I I -1 q 111S7.. (

Rehnqu:shed By: Oatotrirne

Relinquished By· Datemme.

R eport Generated: 07/11/2019 02:50 PM

R1rnnrt Version".\.S.28.1 L;:isl Revisen S/131201 !l 3 :20:S2 PM

7/11/2019 1 :45:00 PM Drinking Water Glass 125 ml Amber

7/11/2019 1 :45:00 PM (Grab)

7/11/20191:45:00 PM Drinking Water Glass 125 ml Amber

7/11/20191:45:00 PM (Grab)

Accepled By; OatefTime:

AccepledBy OalefTime

Accepted By DalefTlme·

AA + bisulfate

Na2S203

545 • Cylindrospermin and Anatoxin Subcontracted

() , t.(dC

,C}f'S 546 • Total Microcystins Subcontracted G) • '{ • C.

L/ :7:,5 ; OJ N Notes

Page 2 of 2

Eurofins Eaton AnalyticalRun Log

Run ID: 262038 Method: 545

Sample SiteSample IdType Instrument ID Calibration FileAnalysis DateMatrix

CCL 4354676 CY 071719M545a-CY.mdb07/17/2019 17:38RW

QCS 4354675 CY 071719M545a-CY.mdb07/17/2019 17:53RW

LRB 4355044 CY 071719M545a-CY.mdb07/17/2019 18:07RW

CCM 4354677 CY 071719M545a-CY.mdb07/17/2019 21:18RW

FS 4353985 19070027-02 CY 071719M545a-CY.mdb07/17/2019 23:44DW

CCH 4354678 CY 071719M545a-CY.mdb07/17/2019 23:59RW

0.00

Page 1 of 2 EEA Run ID 262038 / EEA Report # 458552

Page 9 of 14

=::: e u rofi n s I Eaton Analytical

QC Summary Report

EEAID #

RPDUnitsTargetAmountClient IDMethodAnalyteSampleType

DilFactor

RPDLimit

RecoveryLimits

% Recovery

ResultFlag

MRL Extracted Analyzed

False

IS-L-phenylalanine-d5 545 N/ACCL 21674 ug/L 1.0 4354676--- ------50 - 1509921941 07/02/2019 12:30 07/17/2019 17:38False

IS-Uracil-d4 545 N/ACCL 8146 ug/L 1.0 4354676--- ------50 - 1501047851 07/02/2019 12:30 07/17/2019 17:38False

Anatoxin-a 545 0.0300CCL 0.028633 ug/L 1.0 4354676--- ------50 - 150950.03 07/02/2019 12:30 07/17/2019 17:38False

Cylindrospermopsin 545 0.0900CCL 0.09572 ug/L 1.0 4354676--- ------50 - 1501060.09 07/02/2019 12:30 07/17/2019 17:38True

IS-L-phenylalanine-d5 545 N/AQCS 20356 ug/L 1.0 4354675--- ------50 - 1509321941 07/02/2019 12:30 07/17/2019 17:53True

IS-Uracil-d4 545 N/AQCS 8400 ug/L 1.0 4354675--- ------50 - 1501077851 07/02/2019 12:30 07/17/2019 17:53True

Anatoxin-a 545 0.0300QCS 0.586247 ug/L 1.0 4354675--- ------70 - 1301170.5 07/02/2019 12:30 07/17/2019 17:53True

Cylindrospermopsin 545 0.0900QCS 1.31115 ug/L 1.0 4354675--- ------70 - 130871.5 07/02/2019 12:30 07/17/2019 17:53False

IS-L-phenylalanine-d5 545 N/ALRB 19431 ug/L 1.0 4355044--- ------50 - 1508921941 07/17/2019 13:51 07/17/2019 18:07False

IS-Uracil-d4 545 N/ALRB 8093 ug/L 1.0 4355044--- ------50 - 1501037851 07/17/2019 13:51 07/17/2019 18:07False

Anatoxin-a 545 0.0300LRB 0.002141 ug/L 1.0 4355044--- ------------ < 07/17/2019 13:51 07/17/2019 18:07False

Cylindrospermopsin 545 0.0900LRB 0.0 ug/L 1.0 4355044--- ------------ < 07/17/2019 13:51 07/17/2019 18:07True

IS-L-phenylalanine-d5 545 N/ACCM 22141 ug/L 1.0 4354677--- ------50 - 15010121941 07/02/2019 12:30 07/17/2019 21:18True

IS-Uracil-d4 545 N/ACCM 7934 ug/L 1.0 4354677--- ------50 - 1501017851 07/02/2019 12:30 07/17/2019 21:18True

Anatoxin-a 545 0.0300CCM 0.473143 ug/L 1.0 4354677--- ------70 - 130950.5 07/02/2019 12:30 07/17/2019 21:18True

Cylindrospermopsin 545 0.0900CCM 1.23156 ug/L 1.0 4354677--- ------70 - 130821.5 07/02/2019 12:30 07/17/2019 21:18False

IS-L-phenylalanine-d5 545 N/AFS 20220 ug/L 1.0 435398519070027-02 ------50 - 1509221941 07/17/2019 13:51 07/17/2019 23:44False

IS-Uracil-d4 545 N/AFS 9673 ug/L 1.0 435398519070027-02 ------50 - 1501237851 07/17/2019 13:51 07/17/2019 23:44False

Anatoxin-a 545 0.0300FS 0.0300 ug/L 1.0 435398519070027-02 ------------ < 07/17/2019 13:51 07/17/2019 23:44False

Cylindrospermopsin 545 0.0900FS 0.0900 ug/L 1.0 435398519070027-02 ------------ < 07/17/2019 13:51 07/17/2019 23:44True

IS-L-phenylalanine-d5 545 N/ACCH 21574 ug/L 1.0 4354678--- ------50 - 1509821941 07/02/2019 12:30 07/17/2019 23:59True

IS-Uracil-d4 545 N/ACCH 8389 ug/L 1.0 4354678--- ------50 - 1501077851 07/02/2019 12:30 07/17/2019 23:59True

Anatoxin-a 545 0.0300CCH 1.961 ug/L 1.0 4354678--- ------70 - 130982.0 07/02/2019 12:30 07/17/2019 23:59True

Cylindrospermopsin 545 0.0900CCH 5.37284 ug/L 1.0 4354678--- ------70 - 130906.0 07/02/2019 12:30 07/17/2019 23:59


Page 10 of 14

D

D

.___.ODD□□ DDDDDDDD

DODD□




FS 4353986 19070027-02 FH 07/22/2019 08:57DW

LRB 4358071 FH 07/22/2019 08:57RW

LRB 4358072 FH 07/22/2019 08:57RW

LFB 4358073 FH 07/22/2019 08:57RW

LFB 4358074 FH 07/22/2019 08:57RW

CCL 4358075 FH 07/22/2019 08:57RW

QCS 4358076 FH 07/22/2019 08:57RW

FS 4353986 19070027-02 FH 07/22/2019 08:57DW

LRB 4358071 FH 07/22/2019 08:57RW

LRB 4358072 FH 07/22/2019 08:57RW

0.00


Page 11 of 14


QC Summary Report

EEAID #


DilFactor

RPDLimit

RecoveryLimits

% Recovery

ResultFlag


True

Total Microcystins & Nodularins 546 0.300FS 0.300 ug/L 1.0 435398619070027-02 ------------ < 07/19/2019 13:30 07/22/2019 08:57True

Percent CV 546 N/AFS 2 % 1.0 435398619070027-02 ------------ 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300LRB 0.0 ug/L 1.0 4358071--- ------------ < 07/19/2019 13:30 07/22/2019 08:57False

Percent CV 546 N/ALRB 1.6 % 1.0 4358071--- ------------ 07/19/2019 13:30 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300LRB 0.0 ug/L 1.0 4358072--- ------------ < 07/19/2019 13:30 07/22/2019 08:57True

Percent CV 546 N/ALRB 1.699999 % 1.0 4358072--- ------------ 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300LFB 0.538 ug/L 1.0 4358073--- ------60 - 1401080.5 07/19/2019 13:30 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300LFB 0.562 ug/L 1.0 4358074--- ------60 - 1401120.5 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300CCL 0.239999 ug/L 1.0 4358075--- ------50 - 150800.3 --- 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300QCS 0.678 ug/L 1.0 4358076--- ------70 - 1301130.6 --- 07/22/2019 08:57


Page 12 of 14

1~~rnrnrnt ~ D

D

=O==ll==O==ll==ll=]D[ =

ll=====ll=ll==l~l=l~~l=□[ =

....______.____ODD□□--□-

Sample Type Key

Type (Abbr.) Sample Type Type (Abbr.) Sample Type

CCH Continuing Calibration High

CCL Continuing Calibration Low

CCM Continuing Calibration Mid

FS Field Sample

LFB Laboratory Fortified Blank

LRB Laboratory Reagent Blank

QCS Quality Control Sample

1.00

Page 13 of 14

END OF REPORT

Page 14 of 14

LABORATORY REPORT

This report may not be reproduced, except in full, without written approval from EEA.

If you have any questions concerning this report, please do not hesitate to call us at (800) 332-4345 or (574) 233-4777.

Page 1 of 14

=::: e u ro fi n s I Eaton Analytical

Revision date: 03/14/2019

STATE CERTIFICATION LIST

State Certification State Certification Alabama 40700 Missouri 880 Alaska IN00035 Montana CERT0026 Arizona AZ0432 Nebraska NE-OS-05-04

Arkansas IN00035 Nevada IN00035 California 2920 New Hampshire* 2124 Colorado IN00035 New Jersey* IN598

Colorado Radiochemistry IN00035 New Mexico IN00035 Connecticut PH-0132 New York* 11398 Delaware IN035 North Carolina 18700 Florida* E87775 North Dakota R-035 Georgia 929 Ohio 87775 Hawaii IN035 Oklahoma D9508 Idaho IN00035 Oregon (Primary AB)* 4074

Illinois* 200001 Pennsylvania* 68-00466 Illinois Microbiology 17767 Puerto Rico IN00035

Illinois Radiochemistry IN00035 Rhode Island LAO00343 Indiana Chemistry C-71-01 South Carolina 95005

Indiana Microbiology M-76-07 South Dakota IN00035 Iowa 098 Tennessee TN02973

Kansas* E-10233 Texas* T104704187-18-12 Kentucky 90056 Texas/TCEQ TX207

Louisiana* LA014 Utah* IN00035 Maine IN00035 Vermont VT-8775

Maryland 209 Virginia* 460275 Massachusetts M-IN035 Washington C837

Michigan 9926 West Virginia 9927 C Minnesota* 018-999-338 Wisconsin 999766900 Mississippi IN035 Wyoming IN035

EPA IN00035 *NELAP/TNI Recognized Accreditation Bodies

Page 2 of 14

~:: eu rofi ns I Eaton Analytical

Laboratory Report

Client:

Attn:

Report:

Priority:

Status:

PWS ID:


Ed Watson


458551

FL6290388

Standard Written

Final

Tampa, FL 33619

Sample Event Code:

PWS Facility ID:

Sampling Point ID:

00001

0388001

SE3

Lab ID Code: IN00035


EEA ID #


CollectedBy:

ReceivedDate / Time

4353966 19070027-01 545 07/11/19 09:40 Client 07/16/19 10:15

4353967 19070027-01 546 07/11/19 09:40 Client 07/16/19 10:15

Sample Information

Report Summary

Detailed quantitative results are presented on the following pages. The results presented relate only to the samples provided for analysis.



Client Name:

Report #:


458551


09/10/2019

Page 1 of 2

Page 3 of 14


I I


Client Name: Report #: 458551Hillsborough County Board of County Commissioners

Sampling Point ID: PWS ID: FL62903880388001

UCMR Result Summary

AnalyteID #

Analyte Method MRL† Result Units PreparationDate

AnalyzedDate

EEAID #

3311 Anatoxin-a 545 0.0300 < 0.0300 ug/L 07/17/19 13:51 07/17/19 23:30 4353966

3302 Cylindrospermopsin 545 0.0900 < 0.0900 ug/L 07/17/19 13:51 07/17/19 23:30 4353966

3301 Total Microcystins & Nodularins 546 0.300 < 0.300 ug/L 07/19/19 13:30 07/22/19 08:57 4353967

CVTM Percent CV 546 N/A 1.80 % 07/19/19 13:30 07/22/19 08:57 4353967

† EEA has demonstrated it can achieve these report limits in reagent water, but can not document them in all sample matrices.

Page 2 of 2

Page 4 of 14

II I

~

Lab Definitions

Continuing Calibration Check Standard (CCC) / Continuing Calibration Verification (CCV) / Initial Calibration Verification Standard (ICV) / Initial Performance Check (IPC) - is a standard containing one or more of the target analytes that is prepared from the same standards used to calibrate the instrument. This standard is used to verify the calibration curve at the beginning of each analytical sequence, and may also be analyzed throughout and at the end of the sequence. The concentration of continuing standards may be varied, when prescribed by the reference method, so that the range of the calibration curve is verified on a regular basis. CCL, CCM, and CCH are the CCC standards at low, mid, and high concentration levels, respectively.

Internal Standards (IS) - are pure compounds with properties similar to the analytes of interest, which are added to field samples or extracts, calibration standards, and quality control standards at a known concentration. They are used to measure the relative responses of the analytes of interest and surrogates in the sample, calibration standard or quality control standard.

Laboratory Duplicate (LD) - is a field sample aliquot taken from the same sample container in the laboratory and analyzed separately using identical procedures. Analysis of laboratory duplicates provides a measure of the precision of the laboratory procedures.

Laboratory Fortified Blank (LFB) / Laboratory Control Sample (LCS) - is an aliquot of reagent water to which known concentrations of the analytes of interest are added. The LFB is analyzed exactly the same as the field samples. LFBs are used to determine whether the method is in control. FBL, FBM, and FBH are the LFB samples at low, mid, and high concentration levels, respectively.

Laboratory Method Blank (LMB) / Laboratory Reagent Blank (LRB) - is a sample of reagent water included in the sample batch analyzed in the same way as the associated field samples. The LMB is used to determine if method analytes or other background contamination have been introduced during the preparation or analytical procedure. The LMB is analyzed exactly the same as the field samples.

Laboratory Trip Blank (LTB) / Field Reagent Blank (FRB) - is a sample of laboratory reagent water placed in a sample container in the laboratory and treated as a field sample, including storage, preservation, and all analytical procedures. The FRB/LTB container follows the collection bottles to and from the collection site, but the FRB/LTB is not opened at any time during the trip. The FRB/LTB is primarily a travel blank used to verify that the samples were not contaminated during shipment.

Matrix Spike Duplicate Sample (MSD) / Laboratory Fortified Sample Matrix Duplicate (LFSMD) - is a sample aliquot taken from the same field sample source as the Matrix Spike Sample to which known quantities of the analytes of interest are added in the laboratory. The MSD is analyzed exactly the same as the field samples. Analysis of the MSD provides a measure of the precision of the laboratory procedures in a specific matrix. SDL, SDM, and SDH / LFSMDL, LFSMDM, and LFSMDH are the MSD or LFSMD at low, mid, and high concentration levels, respectively.

Matrix Spike Sample (MS) / Laboratory Fortified Sample Matrix (LFSM) - is a sample aliquot taken from field sample source to which known quantities of the analytes of interest are added in the laboratory. The MS is analyzed exactly the same as the field samples. The purpose is to demonstrate recovery of the analytes from a sample matrix to determine if the specific matrix contributes bias to the analytical results. MSL, MSM, and MSH / LFSML, LFSMM, and LFSMH are the MS or LFSM at low, mid, and high concentration levels, respectively.

Quality Control Standard (QCS) / Second Source Calibration Verification (SSCV) - is a solution containing known concentrations of the analytes of interest prepared from a source different from the source of the calibration standards. The solution is obtained from a second manufacturer or lot if the lot can be demonstrated by the manufacturer as prepared independently from other lots. The QCS sample is analyzed using the same procedures as field samples. The QCS is used as a check on the calibration standards used in the method on a routine basis.

Reporting Limit Check (RLC) / Initial Calibration Check Standard (ICCS) - is a procedural standard that is analyzed each day to evaluate instrument performance at or below the minimum reporting limit (MRL).

Surrogate Standard (SS) / Surrogate Analyte (SUR) - is a pure compound with properties similar to the analytes of interest, which is highly unlikely to be found in any field sample, that is added to the field samples, calibration standards, blanks and quality control standards before sample preparation. The SS is used to evaluate the efficiency of the sample preparation process.

Page 1 of 1

Page 5 of 14

Laboratory Report

Client:

Attn:

Report:

Priority:

Status:

PWS ID:


Ed Watson


458551

FL6290388

Standard Written

Final

Tampa, FL 33619


EEA ID #


CollectedBy:

ReceivedDate / Time

4353965 19070027-01 544 07/11/19 09:40 Client 07/16/19 10:15

Sample Information

Report Summary

Method 544 analysis will be performed only if the associated Screening Microcystin by EPA 546 sample result is >=0.3 ug/L.




09/10/2019

Page 1 of 1

Page 6 of 14



of 14

Subcontracted C hain of Custody

-di 3& 179g

--------------

7 I IJ I I 111111111111 fl I IJI Cosmme, Nom,w;;;;~~/_ Environmental Labo

ratory

~ p .

Work Order: 19070027 roiecr NW

· UCMR4-CYN

Hillsborough County


Sl!)TipletD·

•o7nor Address

~Oi-002

,ddress

• 9010021-0·

Address

Collector

19070U2 NI• ·1

Address

Collector

1.90711027 •0l-5

Address

Collector

'9070027 -02-1

Address

Collector

19070027-02·2

Address

Collector

Public Ut1l1ti€S

Customer

...Northwest Hillst:>orough CWS

1'7';! 16 N Dale '-,11abry Hwy

Graysor., Jusun

Northwest H1llsb0rough CWS

17316 N Dale Mabry Hwy

Grayson Justin

Nortn,vest H1llsbocough CWS

17316 N Dale Mabry Hwy

Grayson, Just in

Northwest H11lsbo:ovgt. C\i\'S

17316 N Dale Mabry HIN}

Grayson, Justm

Northwest Hillsborough CWS

17316 N Dale Mat>ry Hwy

Grayson. J1.,1sti11

Nortnwest Hillsborough cws

12950 t=awnndge Blvd

Grayson. Justin

Northwest Hillsborough CWS

12950 Fawnridge Blvd

Grayson, Justin


Phone No. (813) 272-5977 x 43635

PWS~ Site Collection Date/Time:

6290388 LPWTP· Start: 7 111/2019 9 40'0

0 AM

002 End: 7 /1 i/2019 9·40 00 AM

Title Enwonmenta, Soec1al1s1 I

5290388 LPWrP-Slart· 7/ 11/2019 9:40 0

0 AM

002 End 7/11/2019 9·40 00 AM

Title E~·.-ironmental Specialist I

6290388 LPWTP-Start· 7/ 11/2019 9 40 0

0 AM

002 End 7/1112019 9 40 00 AM

Title C.nv,ronme-,tal Specialtst I

--529n~as LPWTP S

tan 711112019 9 40 00 AM

002 End 7/11/2019 9 40 00 AM

Title Er.wonmental Spec1ahst I

6290388 LPWTP-Stan: 7/ 11/2019 9 40 0

0 AM

002 End 7/11/2019 9 40:00 AM

Title Environmenta1 Specialist I

6290388 NWPWF-SIan 7/11/2019 1 45

00 PM

002 End 7/11i2019 1 45·00 PM

nue Environmenlal Specialist I

6290368 NWPWF-Start 7111/2019 1.45 00 P

M

002 End· 7/11/2019 1 45·00 PM

Tille Environmental Spec1ailsl I

6290386 NWPWF-Stan: 7/11/2019 1 -15 00

PM

002 End· 111112019 1 45·oo PM

Contact Stanley Joh

Scheduled Sample NW UCMR4 ·CYN

Scienti ' n (Chief Environment

h~~ ~

Phone (B13) 272-5977 X 43635

Subcontracted Vendor Eurofins Eaton Analy

t'c I P

I bLC I a

rofile #

Sample Information ~------

Sample Type . Container Type

Drinking Waler Glass 250 ml Amber

(Grab)

Dn~k1ng Water Glass 250 ml Amber

(Grab)

Dnnk1n9 Water Glass 250 ml Amber

(Grab)

Dnskmg Water Glass 125 ml Ambe1

(Grab)

Octnk,ng Water Glass 125 ml Amber

(Grab)

Dnr.ktng Water Gfess 250 mL Ambe,

(Grab)

Drinking Water Glass 250 ml Amber

(Grab)

Drinking Water Glass 250 ml Ambei

(Grab)

Preservauve. Analysis Method

Tnzma • 2CJ acetam,de

+AA+ EDTA 544 . M1crocysuns Subcon

lrac•ed

UCMR List ·

L/7,rj Tnzma • 2CI acetam1de +AA .. EDTA

-Tnzma + 2CI acetam1de

•AA• EDTA

AA ~ b1sulfate -

~t Cyhndrosperm1n and Anatox,n u ontracted

=i -3539(tl(I Na2S203 546 -To1a1 M1crocysuns Subcontracted

lJ~S30(;7 Tnzma • 2Ci aceJamlde

•AA• EDTA ~C4,;RM~~:cysnns Subcontracted

-

rrizma • 2CI ace1am1de

•AA+ EDTl\

rrizma • 2CI acetamide

+AA+ EDTA

Anal)l?es.

3. '{•G

f~ ~I-~

Rt..,,~ c.n&;,.,._ AM,w

/, 0 ~

R.c.,s,d t.ccJ. Cit/,,,. I /IL

o.c;, 'c R

of 14

Subcontracted Chain of Custody ----- - --------"7 i--------~----- - -----

11111111 Ill lllll II I Ill Hillsborough county Public Utilities

En vironmental Laboratory

9456 E Columb us D r Tam pa, F L 3 3619

Certific a tion Numb er : E44104

Phone N o (81 3) 2 72-5977 x 43635

Work Order: 19070027

Scheduled Sample NW UCMR4-CYN Set Name

Subcontracted Vendor Eurofins Eaton Ana lytica l

Sample Information

Customer Northwesl Hillsborough CWS

Proiect

Contact

Phone

Profile II

NW - UCMR4-CYN

Stanley, John (Chief Environmental Sc1enI1st)

(813) 272-5977 X 43635

PWS# Sile Collecuon DaterTime Sample Type Container Type Preservative Analysis Method A ly

,ner: na tes

le ID Gus~ Samp e-.... nn-,oo ,.n,.,o,,.,c e ....... ..,., 1 1, .... n1n 1 , A c -nn

cu. 11 n ...... l,,_..., ,,.,_.__ '"''~ ~ - --- · -

19070027 -02-4

Address

Collector

19070027 02-5

6ddress

i~1-z:· Re ll"IQ'.;~ .. c~ 81

R'-- ,..,.,~ .. ~6,

est Hillsborough CWS NorthW

Fawnridge Blvd 12950

Grayson, Juslin

Northwest Hillsborough cws

12950 Fawnndge Blvd

,on Justin ,--

-✓--,, t

6290~HH NWPWF-002

Stan.

End

Title Environmental Spec1al1s1 I

6290388 NWPWF-002

Stan

Fnd

Title Environmental Specialist I

Oaterm~

t - I I - I Cf 11157__ 0.i(e,...,...,e

Date.'ir:re

R eport Generated 07111/201 9 0 2 :5 0 P M

R1mor1 VP.rstnn 1 S ?A 1 I ;:iSI RP.visP.d S/13/?01 !l 3 ?O S? PM

7/11/2019 1:45:00 PM

7/11/2019 1 45:00 PM

7/11/2019 14500 PM

7/11/2019145.00 PM

Acceplea By

Accec,teo S\

A.cc.er,le~ Bt

Drinking Water

(Grab)

Drinking Water

(Grab )

Glass 125 ml Amber

Glass 125 ml Amber

O•·erT1ft'e

:::>a·e ltn·e

On:e f,,-.e

AA • bisulfate

Na2S203

545 • Cylindrospermin and Anatox1n Subcontracted

546 • Total Mrcrocyst,ns Subcontracted

No:es

Page 2 of




CCL 4354676 CY 071719M545a-CY.mdb07/17/2019 17:38RW

QCS 4354675 CY 071719M545a-CY.mdb07/17/2019 17:53RW

LRB 4355044 CY 071719M545a-CY.mdb07/17/2019 18:07RW

CCM 4354677 CY 071719M545a-CY.mdb07/17/2019 21:18RW

FS 4353966 19070027-01 CY 071719M545a-CY.mdb07/17/2019 23:30DW

CCH 4354678 CY 071719M545a-CY.mdb07/17/2019 23:59RW

0.00


Page 9 of 14


QC Summary Report

EEAID #


DilFactor

RPDLimit

RecoveryLimits

% Recovery

ResultFlag


False

IS-L-phenylalanine-d5 545 N/ACCL 21674 ug/L 1.0 4354676--- ------50 - 1509921941 07/02/2019 12:30 07/17/2019 17:38False

IS-Uracil-d4 545 N/ACCL 8146 ug/L 1.0 4354676--- ------50 - 1501047851 07/02/2019 12:30 07/17/2019 17:38False

Anatoxin-a 545 0.0300CCL 0.028633 ug/L 1.0 4354676--- ------50 - 150950.03 07/02/2019 12:30 07/17/2019 17:38False

Cylindrospermopsin 545 0.0900CCL 0.09572 ug/L 1.0 4354676--- ------50 - 1501060.09 07/02/2019 12:30 07/17/2019 17:38True

IS-L-phenylalanine-d5 545 N/AQCS 20356 ug/L 1.0 4354675--- ------50 - 1509321941 07/02/2019 12:30 07/17/2019 17:53True

IS-Uracil-d4 545 N/AQCS 8400 ug/L 1.0 4354675--- ------50 - 1501077851 07/02/2019 12:30 07/17/2019 17:53True

Anatoxin-a 545 0.0300QCS 0.586247 ug/L 1.0 4354675--- ------70 - 1301170.5 07/02/2019 12:30 07/17/2019 17:53True

Cylindrospermopsin 545 0.0900QCS 1.31115 ug/L 1.0 4354675--- ------70 - 130871.5 07/02/2019 12:30 07/17/2019 17:53False

IS-L-phenylalanine-d5 545 N/ALRB 19431 ug/L 1.0 4355044--- ------50 - 1508921941 07/17/2019 13:51 07/17/2019 18:07False

IS-Uracil-d4 545 N/ALRB 8093 ug/L 1.0 4355044--- ------50 - 1501037851 07/17/2019 13:51 07/17/2019 18:07False

Anatoxin-a 545 0.0300LRB 0.002141 ug/L 1.0 4355044--- ------------ < 07/17/2019 13:51 07/17/2019 18:07False

Cylindrospermopsin 545 0.0900LRB 0.0 ug/L 1.0 4355044--- ------------ < 07/17/2019 13:51 07/17/2019 18:07True

IS-L-phenylalanine-d5 545 N/ACCM 22141 ug/L 1.0 4354677--- ------50 - 15010121941 07/02/2019 12:30 07/17/2019 21:18True

IS-Uracil-d4 545 N/ACCM 7934 ug/L 1.0 4354677--- ------50 - 1501017851 07/02/2019 12:30 07/17/2019 21:18True

Anatoxin-a 545 0.0300CCM 0.473143 ug/L 1.0 4354677--- ------70 - 130950.5 07/02/2019 12:30 07/17/2019 21:18True

Cylindrospermopsin 545 0.0900CCM 1.23156 ug/L 1.0 4354677--- ------70 - 130821.5 07/02/2019 12:30 07/17/2019 21:18False

IS-L-phenylalanine-d5 545 N/AFS 20951 ug/L 1.0 435396619070027-01 ------50 - 1509521941 07/17/2019 13:51 07/17/2019 23:30False

IS-Uracil-d4 545 N/AFS 8789 ug/L 1.0 435396619070027-01 ------50 - 1501127851 07/17/2019 13:51 07/17/2019 23:30False

Anatoxin-a 545 0.0300FS 0.0300 ug/L 1.0 435396619070027-01 ------------ < 07/17/2019 13:51 07/17/2019 23:30False

Cylindrospermopsin 545 0.0900FS 0.0900 ug/L 1.0 435396619070027-01 ------------ < 07/17/2019 13:51 07/17/2019 23:30True

IS-L-phenylalanine-d5 545 N/ACCH 21574 ug/L 1.0 4354678--- ------50 - 1509821941 07/02/2019 12:30 07/17/2019 23:59True

IS-Uracil-d4 545 N/ACCH 8389 ug/L 1.0 4354678--- ------50 - 1501077851 07/02/2019 12:30 07/17/2019 23:59True

Anatoxin-a 545 0.0300CCH 1.961 ug/L 1.0 4354678--- ------70 - 130982.0 07/02/2019 12:30 07/17/2019 23:59True

Cylindrospermopsin 545 0.0900CCH 5.37284 ug/L 1.0 4354678--- ------70 - 130906.0 07/02/2019 12:30 07/17/2019 23:59


Page 10 of 14

D

D

.___.ODD□□ DDDDDDDD

DODD□




FS 4353967 19070027-01 FH 07/22/2019 08:57DW

LRB 4358071 FH 07/22/2019 08:57RW

LRB 4358072 FH 07/22/2019 08:57RW

LFB 4358073 FH 07/22/2019 08:57RW

LFB 4358074 FH 07/22/2019 08:57RW

CCL 4358075 FH 07/22/2019 08:57RW

QCS 4358076 FH 07/22/2019 08:57RW

FS 4353967 19070027-01 FH 07/22/2019 08:57DW

LRB 4358071 FH 07/22/2019 08:57RW

LRB 4358072 FH 07/22/2019 08:57RW

0.00


Page 11 of 14


QC Summary Report

EEAID #


DilFactor

RPDLimit

RecoveryLimits

% Recovery

ResultFlag


True

Total Microcystins & Nodularins 546 0.300FS 0.300 ug/L 1.0 435396719070027-01 ------------ < 07/19/2019 13:30 07/22/2019 08:57True

Percent CV 546 N/AFS 2 % 1.0 435396719070027-01 ------------ 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300LRB 0.0 ug/L 1.0 4358071--- ------------ < 07/19/2019 13:30 07/22/2019 08:57False

Percent CV 546 N/ALRB 1.6 % 1.0 4358071--- ------------ 07/19/2019 13:30 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300LRB 0.0 ug/L 1.0 4358072--- ------------ < 07/19/2019 13:30 07/22/2019 08:57True

Percent CV 546 N/ALRB 1.699999 % 1.0 4358072--- ------------ 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300LFB 0.538 ug/L 1.0 4358073--- ------60 - 1401080.5 07/19/2019 13:30 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300LFB 0.562 ug/L 1.0 4358074--- ------60 - 1401120.5 07/19/2019 13:30 07/22/2019 08:57False

Total Microcystins & Nodularins 546 0.300CCL 0.239999 ug/L 1.0 4358075--- ------50 - 150800.3 --- 07/22/2019 08:57True

Total Microcystins & Nodularins 546 0.300QCS 0.678 ug/L 1.0 4358076--- ------70 - 1301130.6 --- 07/22/2019 08:57


Page 12 of 14

1~~rnrnrnt ~ D

D

=O==ll==O==ll==ll=]D[ =

ll=====ll=ll==l~l=l~~l=□[ =

....______.____ODD□□--□-

Sample Type Key

Type (Abbr.) Sample Type Type (Abbr.) Sample Type

CCH Continuing Calibration High

CCL Continuing Calibration Low

CCM Continuing Calibration Mid

FS Field Sample

LFB Laboratory Fortified Blank

LRB Laboratory Reagent Blank

QCS Quality Control Sample

1.00

Page 13 of 14

END OF REPORT

Page 14 of 14

2019-07-11 NW- Fawn Ridge Cyanotoxins2019-07-11 NW- Lake Park Cyanotoxins

Northwest Cyantoxin Results 7-11-19€¦ · 2019-07-11 · Colorado Radiochemistry IN00035 New...

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