Normal-Phase Columns · YMC Co., Ltd. offers normal-phase columns packed with bare silica gel or...

YMC GENERAL CATALOG

3Normal-Phase Columns

Types and characteristics ofnormal-phase columns

YMC-Pack SIL、SIL-06YMC-Pack CNYMC-Pack Diol-NPYMC-Pack Polyamine ⅡYMC-Pack NH2

YMC-Pack PA-GYMC-Pack PVA-SilYMC columns for SFC separation

80,81

82,8384,8586,8788,89

90909192

79

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80

3

Norm

al-Phase C

olumns

Types and characteristics of normal-phase columns

Product NamePore size（Å）

Particlesize

（µm）

Usable pH range Characteristics Page

YMC-Pack seriesSIL 120

3

2.0 ～ 7.5

・ Standard normal-phase column using non-polar mobile phase

・ For separation of various isomers and compounds with similar structures

82,835

10

SIL-06 60 510

CN 1205 ・ For normal-phase separation using non-polar mobile phase similar to that used with silica gel

・ Can be used in reversed-phase mode using aqueous mobile phase 84,8510

Diol-NP60

5・ For normal-phase separation using non-polar mobile phase・ Different separation characteristics from bare silica gel・ Useful for normal-phase separation (HILIC) of hydrophilic compounds

86,87120

Polyamine Ⅱ 120 5 ・ For normal-phase separation using non-polar mobile phase・ For separation of sugars and vitamins using aqueous mobile phase 88,89

NH2 120 5 ・ For normal-phase separation using non-polar mobile phase・ For separation of sugars using aqueous mobile phase 90

PA-G 120 5 4.0 ～ 7.5 ・ For separation of sugars using aqueous mobile phase 90

PVA-Sil 120 5 2.0 ～ 9.5 ・ Silica gel chemically bonded with polyvinyl alcohol・ Excellent for supercritical fluid chromatography 91

Selection of YMC-Pack CN separation modes

10 20 min

1

2

3

4

0

（S930322B）

Reversed-phase mode

Column ：YMC-PackCN(5µm,120Å)250×4.6mmI.D.

Eluent ：acetonitrile/water(40/60)Flowrate ：0.6mL/minPressure ：5.6MPaTemperature：30℃Detection ：UVat254nm,0.32AUFSInjection ：3µL(0.25mg/mL)

1

2

3

4

10 20 min0

（S930319D）

Normal-phase mode

Column ：YMC-PackCN(5µm,120Å) 250×4.6mmI.D.

Eluent ：hexane/methanol/dichloromethane(75/20/5)Flowrate ：0.6mL/minPressure ：2.6MPaTemperature：30℃Detection ：UVat254nm,0.32AUFSInjection ：3µL（0.25mg/mL）

AnexampleofanalysisusingYMC-PackCNinwhichthesamesamplesareanalyzedinbothnormal-phaseandreversed-phasemodes,isshownontheleft.YMC-PackCNcanbeusedinbothnormal-phaseandreversed-phasemodes,sinceithascyanopropylgroupsofmediumpolarity.Itcanbeusedinreversed-phasemodewithaqueousmobilephaseofhigherpolaritythanthestationaryphase.Itcanalsobeusedinnormal-phasemodewithlow-polaritymobilephasesuchashexane.Theorderofelutionwillbereverseddependingontheseparationmode.

4.

O

CH 3

O

3.

O

CH OH2

O

2.

O

CH OH

HO

2

O

1.

O

CH OH

OHHO

2

O

Progesterone

Deoxycorticosterone

Corticosterone

Hydrocortisone

High

Polarity

Low

Types and characteristics of normal-phase columnsNormal-phase chromatography is useful for the separation of positional isomers that are difficult to separate in reversed-phase mode.YMC Co., Ltd. offers normal-phase columns packed with bare silica gel or packed with silica gel modified with polar groups.

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Norm

al-Phase C

olumns

Separation characteristics of normal-phase columns

5 10 15 200 min

1

2

3

4

（A010606A）

YMC-Pack SIL（5 µm, 120 Å）

�1.�Di-n-octyl�phthalate2.�Di-n-butyl�phthalate3.�Diethyl�phthalate4.�Dimethyl�phthalate

Silica gel

Acomparisonofelutionbehaviorofvariousnormal-phasecolumnsinseparationofphthalateestersisshown.Generally,innormal-phasechromatography,thehigherthepolarityofthestationaryphasesurface,thegreatertheretentionofsolutes.Thepolarityofchemicallybondedpackingmaterialsislowerthanthatofsilicagelandthesepackingmaterialshavedifferentseparationcharacteristics.

Chemically bonded packing material

1

2 3

4

YMC-Pack Polyamine Ⅱ（5 µm, 120 Å）

5 10 150 min（A010607A）

1

2

34

YMC-Pack Diol-NP（5 µm, 120 Å）

5 100 min（A010606G）

1

2

3

4

YMC-Pack CN（5 µm, 120 Å）

5 100 min（A010606C）

Column ：250×4.6mmI.D.Eluent ：hexane/ethylacetate(90/10)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat254nm

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Norm

al-Phase C

olumns

Analytical columns

YMC-Pack SIL, YMC-Pack SIL-06● High quality spherical porous silica gel● Normal-phase separation due to surface silanol groups● Two different pore sizes are available● Useful for separation of compounds with similar structures

S t a n d a r d n o r m a l - p h a s e c o l u m nYMC-PackSILissuitableforseparationoffat-solublecompoundsusingnon-polarmobilephaseandseparationofpositionalisomersthataredifficulttoseparateinreversed-phasemode.SIL-06(porediameter60Å)hasgreaterabsorptionpropertiesthanSIL-12(porediameter120Å)duetoitslargerspecificsurfacearea,andis

generallyusefulforseparatingnaturalproductswithalowmolecularweight.Althoughwateroraceticacidcanbeusedasanadditivetothemobilephase,theyarelikelytobindtosilanol.Inconsiderationofseparationreproducibility,itispreferabletohaveonecolumndedicatedtothemobilephasecontainingwateroraceticacid.

■Poresize：60Å，120Å■UsablepHrange：2.0〜 7.5

1

2

3 4

10 min0

Tocopherol isomers1.�α-Tocopherol2.�β-Tocopherol3.�γ-Tocopherol4.�σ-Tocopherol

Column ：YMC-PackSIL(5µm,120Å) 150×4.6mmI.D.

Eluent ：hexane/THF/aceticacid(97/3/0.25)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat295nm

100 20

1

2

min

Hydrocortisone and prednisone

O

O

Prednisone

1.

3CH

3CH

2CH

C=O

OH

OH

O

HO

Hydrocortisone

2.

3CH

3CH

2CH

C=O

OH

OH

Column ：YMC-PackSIL(5µm,120Å) 150×4.6mmI.D.

Eluent ：chloroform/2-propanol/aceticacid(95/5/1)

Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat250nm

A p p l i c a t i o n （G910523S）

A p p l i c a t i o n （K940530C）

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Norm

al-Phase C

olumns

SIL（Pore size 120 Å）

Analytical columnsParticle size（µm）

Pore size（Å）

Column sizeinnerdiameter×length（mm） Product number

S-3 120 4.6×100 SL12S03-1046WT4.6×150 SL12S03-1546WT6.0×100 SL12S03-1006WT6.0×150 SL12S03-1506WT

S-5 120 4.6×100 SL12S05-1046WT4.6×150 SL12S05-1546WT4.6×250 SL12S05-2546WT4.6×300 SL12S05-3046WT6.0×100 SL12S05-1006WT6.0×150 SL12S05-1506WT6.0×250 SL12S05-2506WT6.0×300 SL12S05-3006WT


Semi-preparative columnsParticle size（µm）

Pore size（Å）


S-5 120 10×150 SL12S05-1510WT10×250 SL12S05-2510WT10×300 SL12S05-3010WT20×100 SL12S05-1020WT20×150 SL12S05-1520WT20×250 SL12S05-2520WT

S-10 120 10×150 SL12S11-1510WT10×250 SL12S11-2510WT10×300 SL12S11-3010WT20×150 SL12S11-1520WT20×250 SL12S11-2520WT

SeeP152forpreparativecolumnsotherthanthoselistedabove.

Guard cartridge columns (3/pack)S-5 120 4.0×23 SL12S05-G304CC

Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.

Cartridge holderCartridge holder(inner diameter: 4.0 mm)

XPCHW

Guard columnsParticle size（µm）

Pore size（Å）


S-3 120 4.0×10 SL12S03-0104WFG5.0×10 SL12S03-0105WFG

S-5 120 4.0×10 SL12S05-0104WFG5.0×10 SL12S05-0105WFG10×30 SL12S05-0310WTG20×50 SL12S05-0520WTG


SIL-06（Pore size 60 Å）


Pore size（Å）





Pore size（Å）


S-5 60 10×150 SL06S05-1510WT10×250 SL06S05-2510WT10×300 SL06S05-3010WT20×100 SL06S05-1020WT20×150 SL06S05-1520WT20×250 SL06S05-2520WT

S-10 60 10×150 SL06S11-1510WT10×250 SL06S11-2510WT10×300 SL06S11-3010WT20×150 SL06S11-1520WT20×250 SL06S11-2520WT


Guard cartridge columns (3/pack)S-5 60 4.0×23 SL06S05-G304CC



XPCHW


Pore size（Å）




Ordering information

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Norm

al-Phase C

olumns

Analytical columns

YMC-Pack CN● Silica gel chemically bonded with cyanopropyl groups● For both normal-phase and reversed-phase modes● Different separation characteristics from bare silica gel● Faster column equilibration than bare silica gel

Column can be used in both normal -phase and reversed-phase modesYMC-PackCNshowsretentionbehaviorofnormal-phasechromatographywhenitisusedwithlow-polaritymobilephasessuchashexane.SinceYMC-PackCNstationaryphasesurfaceislesspolarthanbaresilicagel,theretentiontimesofanalytesaregenerallyshorterthanwithbaresilicagel.YMC-PackCNisthereforeappropriateforsampleshavingtoostrongretentionwhen

analyzedusingbaresilicagel.Incontrast,YMC-PackCNshowsretentionbehaviorofreversed-phasechromatographywhenitisusedwithhighpolaritymobilephase,suchasmethanolandwater.Althoughseparationmodesareselectableaccordingtothepurposeofseparation,itispreferabletouseonecolumndedicatedforoneseparationmodeinconsiderationofthelifeofthecolumn.

■Poresize：120Å■UsablepHrange：2.0〜 7.5

1

2

3

4 5

10 200 min

Nitroaniline isomers1. 2. 3. 4. 5.

CH3 NO2 NH2NO2

NH2

NO2

NH2

NO2Toluene Nitrobenzene o-Nitroaniline m-Nitroaniline p-Nitroaniline

Column ：YMC-PackCN(5µm,120Å) 250×4.6mmI.D.

Eluent ：hexane/2-propanol(80/20)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat254nm

A p p l i c a t i o n （A010619A）


Pore size（Å）


S-3 120 2.0× 35 CN12S03-H502WT2.0× 50 CN12S03-0502WT2.0× 75 CN12S03-L502WT2.0×100 CN12S03-1002WT2.0×150 CN12S03-1502WT3.0× 50 CN12S03-0503WT3.0×100 CN12S03-1003WT3.0×150 CN12S03-1503WT4.6×100 CN12S03-1046WT4.6×150 CN12S03-1546WT

S-5 120 1.0×150 CN12S05-1501WT1.0×250 CN12S05-2501WT1.5×150 CN12S05-15P5WT1.5×250 CN12S05-25P5WT2.0×150 CN12S05-1502WT2.0×250 CN12S05-2502WT4.6× 75 CN12S05-L546WT4.6×100 CN12S05-1046WT4.6×150 CN12S05-1546WT4.6×250 CN12S05-2546WT4.6×300 CN12S05-3046WT6.0×100 CN12S05-1006WT6.0×150 CN12S05-1506WT6.0×250 CN12S05-2506WT6.0×300 CN12S05-3006WT

Particle size（µm）

Pore size（Å）


S-5 300 1.0×150 CN30S05-1501WT1.0×250 CN30S05-2501WT1.5×150 CN30S05-15P5WT1.5×250 CN30S05-25P5WT2.0×150 CN30S05-1502WT2.0×250 CN30S05-2502WT4.6× 75 CN30S05-L546WT4.6×100 CN30S05-1046WT4.6×150 CN30S05-1546WT4.6×250 CN30S05-2546WT4.6×300 CN30S05-3046WT6.0×100 CN30S05-1006WT6.0×150 CN30S05-1506WT6.0×250 CN30S05-2506WT6.0×300 CN30S05-3006WT

S-10 120 4.6×100 CN12S11-1046WT4.6×150 CN12S11-1546WT4.6×250 CN12S11-2546WT4.6×300 CN12S11-3046WT6.0×100 CN12S11-1006WT6.0×150 CN12S11-1506WT6.0×250 CN12S11-2506WT6.0×300 CN12S11-3006WT

*ShippingsolventforCNismethanol/water(60/40).Pleasetakecareofmiscibilityinnormal-phaseseparation.


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Norm

al-Phase C

olumns


Pore size（Å）


S-5 120 10×150 CN12S05-1510WT10×250 CN12S05-2510WT10×300 CN12S05-3010WT20×100 CN12S05-1020WT20×150 CN12S05-1520WT20×250 CN12S05-2520WT

300 20×150 CN30S05-1520WT20×250 CN30S05-2520WT

S-10 120 10×150 CN12S11-1510WT10×250 CN12S11-2510WT10×300 CN12S11-3010WT20×150 CN12S11-1520WT20×250 CN12S11-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 CN12S03-0102CC

4.0×23 CN12S03-G304CC

S-5 120 1.0×10 CN12S05-0101CC1.5×10 CN12S05-01P5CC2.0×10 CN12S05-0102CC4.0×23 CN12S05-G304CC

300 1.0×10 CN30S05-0101CC1.5×10 CN30S05-01P5CC2.0×10 CN30S05-0102CC4.0×23 CN30S05-G304CC


Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)

XPCHSMW

Cartridge holder(inner diameter: 4.0 mm)

XPCHW


Pore size（Å）


S-5 120 4.0×10 CN12S05-0104WFG5.0×10 CN12S05-0105WFG10×30 CN12S05-0310WTG20×50 CN12S05-0520WTG

300 4.0×10 CN30S05-0104WFG5.0×10 CN30S05-0105WFG10×30 CN30S05-0310WTG20×50 CN30S05-0520WTG

S-10 120 4.0×10 CN12S11-0104WFG5.0×10 CN12S11-0105WFG10×30 CN12S11-0310WTG20×50 CN12S11-0520WTG

*ShippingsolventforCNismethanol/water(60/40).Pleasetakecareofmiscibilityinnormal-phaseseparation.


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Norm

al-Phase C

olumns

Analytical columns

YMC-Pack Diol-NP● Silica gel bonded with dihydroxypropyl group● Two pore sizes of 120 Å and 60 Å are available● Normal-phase separation using non-polar

solvents

● Useful for hydrophilic interaction chromatography (HILIC)

● Different separation characteristics from bare silica gel

D i f f e r e n t s e p a r a t i o n c h a r a c t e r i s t i c s f r o m b a r e s i l i c a g e lYMC-PackDiol-NPshowsretentionbehaviorofnormal-phasechromatographywhenitisusedwithlow-polaritymobilephases.Hydroxylgroupsonthesurfaceofthestationaryphaseactaspolargroups.YMC-PackDiol-NPisaswidely

applicabletonormal-phaseseparationassilicagel.Itisalsousefulincaseswhereseparationoptimizationisdifficulttoachieveusingbaresilicagel.Inaddition,itisavailableforHILICmodeseparationbyusingorganic/watermobilephases.

■Poresize：120Å，60Å■UsablepHrange：2.0〜 7.5

Separation characteristics of Diol-NP and SIL

5 10 15 200 minA010526A

1

2

3

4

5 6

YMC-Pack Diol-NP（5 µm, 120 Å）

5 10 150 minA010526C

1

23

4

5

6YMC-Pack SIL（5 µm, 120 Å）

Column ：250×4.6mmI.D.Eluent ：hexane/ethanol(80/20)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat254nm

DifferencesinseparationcharacteristicsofDiol-NPandSILforseparatingphenolsareshownontheleft.Diol-NPshowslongerretentionandbetterseparationthanSILinseparatingthesecompounds.

OH

OHOH

OH

HO

OH

HO

HO

OH

OH

OH

HO OH

Phenol

1.

2.

Catechol

3.

Resorcinol

4.

Hydroquinone

5.

Pyrogallol

6.

Phloroglucinol

Available as a HILIC column for separation of hydrophilic compounds

1

5

4

32

6

0 105 min15R090116P

Diol-NPisavailableforHILICmodeseparationbyusingorganic/watermobilephases.

1. Uracil 2. Uridine 3. Adenosine 4. Cytosine 5. Cytidine 6. Guanosine

NH

HN

O

O

N

HN

OH

O

OH

HOH2CO

O

N

N

N

N

OH

O

OH

HOH2C

NH2

NH

N

O

NH2

N

N

OH

O

OH

HOH2CO

NH2

HN

N

N

N

O

OH

O

OH

HOH2CH2N

Column ：YMC-PackDiol-NP(5µm,120Å)150×2.0mmI.D.

Eluent ：water/acetonitrile(10/90)containing10mMCH3COONH4Flowrate ：0.2mL/minTemperature：30℃Detection ：UVat254nm

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Norm

al-Phase C

olumns

Analytical columnsPacking material


Pore size（Å）


Diol-60 S-5 60 3.0×150 DN06S05-1503WT4.6×100 DN06S05-1046WT4.6×150 DN06S05-1546WT4.6×250 DN06S05-2546WT

Diol-120 S-5 120 2.0×150 DN12S05-1502WT3.0×150 DN12S05-1503WT4.6× 50 DN12S05-0546WT4.6×100 DN12S05-1046WT4.6×150 DN12S05-1546WT4.6×250 DN12S05-2546WT

Semi-preparative columnsPacking material


Pore size（Å）


Diol-60 S-5 60 20×150 DN06S05-1520WT20×250 DN06S05-2520WT

Diol-120 S-5 120 20×150 DN12S05-1520WT20×250 DN12S05-2520WT

*ShippingsolventforDiol-NPishexane/2-propanol(99.5/0.5).Incaseofusingeluentincludingwater,takecareofmiscibility.

N

N N

NH2

NH2

H2N

Melamine

1.Analysis of melamine in powdered cheese

(R090127P)

(R090127Q)

(R090127R)

A) Powdered cheese (blank)

C) Standard (0.5 µg/mL)

B) Melamine-spiked powdered cheese (10 mg/kg, final 0.5 µg/mL)

Powdered cheese (1 g)50% aqueous acetonitrile (5 mL)

Sonication for 30 min

Centrifugation (10,000 rpm) for 10 min

Filtration with YMC Duo-Filter (0.2 µm)

Filtration with YMC Duo-Filter (0.2 µm)

Dilution with acetonitrile or eluent

Analysis by HPLC

Extraction procedures for melamineExtraction procedures for melamine

0

60

80

40

20

mAU

min

1

1

0 4321 5

Excellent reproducibility and durability

Theabovedatashowsanalysisofmelamineinpowderedcheese,includinglot-to-lotreproducibilityandrepeatability(durability).Ingeneral,HILICcolumntendstoshowlessreproducibilityordurabilitybutDiol-NPshowsverygoodperformance.HILICcolumnlikebaresilica,zwitterionandaminotypemayadsorbioniccompounds,ontheotherhandDiol-NPhasneutralfunctionalgroupswhichreducenonspecificadsorptions.Therefore,itwouldresultinshowinggoodreproducibilityanddurability.

Lot-to-lot reproducibility

(R090625B)

(R090702A)

(R090625H)

Lot. A

Lot. C

Lot. B

Melamine

min

mAU

0

75

50

25

0 321 4

Repeatability (durability)

(R090129K)(R090129K)

(R090129G)(R090129G)

(R090129H)

(R090129I)

(R090129J)

(R090129F)(R090129F)

0 4 5321 min

1st

200th300th400th500th

100th0

100

600

500

400

300

200

mAU

Melamine

Column ：YMC-PackDiol-NP(5µm,120Å)150×2.0mmI.D.

Eluent ：10mMCH3COONH4/methanol(5/95)Flowrate ：0.2mL/minTemperature：40℃Detection ：UVat210nm


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Norm

al-Phase C

olumns

Analytical columns

YMC-Pack Polyamine Ⅱ● Silica gel chemically bonded with polyamine● The most suitable column for separation of sugars● Solves durability problem of conventional

silica-based amino columns

● Useful for separation of hydrophilic compounds including vitamins

● Useful for separation of fat-soluble compounds using nonaqueous mobile phase

A m i n o c o l u m n w i t h i m p r o v e d d u r a b i l i t yYMC-PackPolyamineIIisasilica-basedpackingbondedwithpolyamine.Itisparticularlyusefulforseparationofsugars.ThecolumnlifetimeofYMC-PackPolyamineⅡinaqueousmobilephaseislongerthanconventionalsilica-basedaminocolumns,andthusis

applicabletoseparationofoligosaccharidesusingmobilephasewithrelativelyhigherwatercontent.Inaddition,YMC-PackPolyamineⅡcanbeusedtoseparateioniccompoundswithacombinationofnormal-phasemodeandweakanionexchangemode.


Excellent durability

0 50 100

10

20

30

Fructose

Glucose

Sucrose

Maltose

Lactose

Continuous flow of water (time, hr)

Retention time (min) 〈Flowconditions〉

Column ：YMC-PackPolyamineⅡ 250×4.6mmI.D.

Eluent ：waterFlowrate ：1.0mL/minTemperature：ambientTime ：100hours

〈Analyticalconditions〉Eluent ：acetonitrile/water(75/25)Flowrate ：1.0mL/minTemperature：26℃Detection ：RI,32×10-6RIU/FS

Eveninahardflowdurabilitytestusingamobilephasethatconsistsonlyofwater,theretentiontimeofsugarschangeslittle.

The most suitable column for separation of sugars, including oligosaccharides

10 20 30 40 min0

1 2

3

45

1. Maltoundecaose（G11）2. Maltododecaose（G12）3. Maltotridecaose（G13）4. Maltotetradecaose（G14）5. Maltopentadecaose（G15）

（T910718A）

Column ：YMC-PackPolyamineⅡ 250×4.6mmI.D.Eluent ：acetonitrile/water(55/45)Flowrate ：1.0mL/minTemperature：26℃Detection ：RI,32×10-6RIU/FS

1

23

45

1. Fructose2. Glucose3. Sucrose4. Maltose5. Lactose

（F030618H）10 20 30 min0

Column ：YMC-PackPolyamineⅡ 250×4.6mmI.D.Eluent ：acetonitrile/water(75/25)Flowrate ：1.0mL/minTemperature：25℃Detection ：RI,32×10-6RIU/FS

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Norm

al-Phase C

olumns

Elution volume of sugars and sugar alcohols

0

5

10

15

20

25

30

35

40

45

50

55

60

65

Glycerol

ErythritolFucose

Xylose

Xylitol

Rhamnose

Arabitol

Frucutose

Mannose（※）

Sorbitol

Mannitol

Glucose

Galactose（※）

Sucrose

Inositol

Maltitol

Maltose

Lactose

Raffinose

Palatinose

Sucralose

Ribitol

75% 80% 85%

Lactitol

Palatinit

Acetonitrile concentration (%)※Not detected with 85% acetonitrile

Elution volume (mL)

YMC-PackPolyamineⅡisapplicableforseparationoffat-solublevitaminsandwater-solublevitaminsasanormal-phasecolumnthatcanbeusedwithwaterorbufferandvariousorganicsolvents.

For normal-phase separation

10

1

2

3

4

0 20 30 min （T920728E）

O

CH

HO

H C

3

CH 3

3

α-Tocopherol

1.

O

HO

H C

CH 3

3

γ-Tocopherol

3.

O

CH

HO3

CH 3

β-Tocopherol

2.

O

HO

CH 3

σ-Tocopherol

4.

Column ：

Eluent ：

Flow rate ：Temperature ：Detection ：

YMC-Pack Polyamine Ⅱ250×4.6 mmI.D.hexane/ethly acetate(70/30)1.0 mL/min30 ℃UV at 295 nm

Tocopherol isomers

（A970603A）min

1

2 3

0 10 20

1. Nicotinic acid2. Erythorbic acid3. L-Ascorbic acid

Column ：

Eluent ：Flow rate ：Temperature ：Detection ：

YMC-Pack Polyamine Ⅱ250×4.6 mmI.D.acetonitrile/50 mM NH4H2PO4 (70/30)1.0 mL/min30 ℃UV at 250 nm

Water-soluble vitamin


Pore size（Å）


S-5 120 4.6×150 PB12S05-1546WT4.6×250 PB12S05-2546WT6.0×150 PB12S05-1506WT6.0×250 PB12S05-2506WT


Pore size（Å）


S-5 120 10×250 PB12S05-2510WT20×250 PB12S05-2520WT

Guard cartridge columns (three-pack)S-5 120 4.0×23 PB12S05-G304CC



XPCHW

Guard columnsS-5 120 4.0×10 PB12S05-0104WFG

5.0×10 PB12S05-0105WFG10×30 PB12S05-0310WTG20×50 PB12S05-0520WTG


第03章.indd 89 11.1.28 10:49:31 AM

90

3

Norm

al-Phase C

olumns

Analytical columns

YMC-Pack NH2

● Silica gel chemically bonded with aminopropyl groups● Useful for separation of sugars● Enables normal-phase mode separation using aqueous or nonaqueous mobile phase

N o r m a l - p h a s e s e p a r a t i o n c o l u m n u t i l i z i n g a m i n o g r o u p sYMC-PackNH2isanormal-phaseseparationcolumnutilizingthepolarityofprimaryaminogroups.Itisalso

applicabletoseparationsutilizingweakanionexchange.YMC-PackNH2isoftenusedforseparationofsugars.


YMC-Pack PA-G● Silica gel chemically bonded with polyamine● Useful for separation of acidic oligosaccharides

N o r m a l - p h a s e s e p a r a t i o n c o l u m n u t i l i z i n g a m i n o g r o u p sYMC-PackPA-Gisusefulforseparationofacidicoligosaccharides.YMC-PackPA-GissimilarselectivityasYMC-PackPA.



Pore size（Å）


S-5 120 4.6×100 NH12S05-1046WT4.6×150 NH12S05-1546WT4.6×250 NH12S05-2546WT4.6×300 NH12S05-3046WT6.0×100 NH12S05-1006WT6.0×150 NH12S05-1506WT6.0×250 NH12S05-2506WT6.0×300 NH12S05-3006WT

Semi-preparative columnsS-5 120 10×150 NH12S05-1510WT

10×250 NH12S05-2510WT10×300 NH12S05-3010WT20×150 NH12S05-1520WT20×250 NH12S05-2520WT


Guard cartridge columns (three-pack)Particle size（µm）

Pore size（Å）


S-5 120 4.0×23 NH12S05-G304CC



XPCHW


Pore size（Å）


S-5 120 4.0×10 NH12S05-0104WFG5.0×10 NH12S05-0105WFG10×30 NH12S05-0310WTG20×50 NH12S05-0520WTG



Pore size（Å）


S-5 120 4.6×150 PG12S05-1546WT4.6×250 PG12S05-2546WT


Guard columnsS-5 120 4.0× 10 PG12S05-0104WFG

第03章.indd 90 11.1.28 10:49:32 AM

3

Norm

al-Phase C

olumns

91

Analytical columns

YMC-Pack PVA-Sil● Vinyl alcohol polymerised silica● High stability and reproducibility● Excellent choice for SFC (Supercritical Fluid Chromatography)

P o l y v i n y l a l c o h o l f u n c t i o n a l i z e d s i l i c aYMC-PackPVA-Sil,bondedwithamonomolecularpolymercoatingofvinylalcohol(PVA),completelycoversbothexternalandinternalsurfacesofthesilicasupport,protectingitagainstaggressive,highpHbuffersand

solvents.ThePVApolymershellonPVA-Sildeactivatesthesilicasupportwhileprovidingahydrophilicsurface.PVA-SilcolumnarealsowellsuitedforSFCseparations.(seepage92forSFC.)

■Poresize：120Å■UsablepHrange：2.0〜 9.5■RecommendedpHrange：2.0〜 7.5

0

Time (min)

SE

S ASG

DG MGDGDGDG

PE

PG PI

PC

SGCER

? ?

8 16 24 32 40

Potato Lipids

Column ：YMC-PackPVA-Sil(5µm,120Å),250×4.6mmI.D.Flowrate ：1to2mL/minMobilePhase：A）2-methylpentane/methyltert-butylether(98/2)

B）2-propanol/acetonitrile/CHCI3/CH3COOH(84/8/8/0.025)C）2-propanol/water/triethylamine(50/50/0.2)

Gradient Tmin：0 5 15 40 40.1 45 50 B%：0 20 52 52 70 0 0 C%：0 0 4 14 0 0 0 Flow(mL/min)：1 1 1 1.4 1.4 2 2

Nebulisertemperature:25℃,Evaporationtemperature：35℃Detector：ELSDSampleS：Sterols CER：CerebrosidesSE：SterolEsters SG：SterylglycosidesMGDG：Monogalactosyldiacylglycerols DGDG：DigalactosyldiacylglycerolsPE：Phosphatidylethanolamine PG：PhosphatidylglycerolsPC：Phosphatidylcholine ASG：AcylsterylglycosidesPI：Phosphatidylinositol DG：Diacylglycerol

A p p l i c a t i o n Literature:W.W.Christie;R.A.Urwin,J.HighResol.Chromatogr.,Vol.18(1995)P97-100

0 5 10 15 20 min

0

5

10

15

20

25mAU

1

23

4

5

6

Nucleotides (in HILIC mode)1.�5'-TMP2.�5'-UMP3.�5'-IMP4.�5'-AMP5.�5'-GMP6.�5'-CMP

Column ：YMC-PackPVA-Sil(5µm,120Å)150×2.0mmI.D.

Eluent ：acetonitrile/100mMCH3COONH4(75/25)Flowrate ：0.2mL/minTemperature：40℃Detection ：UVat254nm

A p p l i c a t i o n (R100208K)



Pore size（Å）


S-5 120 4.6× 50 PV12S05-0546WT4.6×100 PV12S05-1046WT4.6×150 PV12S05-1546WT4.6×250 PV12S05-2546WT

Guard cartridge columns (three-pack) S-5 120 4.0×23 PV12S05-G304CC


Cartridge holderCartridge holder(inner diameter：4.0 mm)

XPCHW

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92

3

Norm

al-Phase C

olumns

Analytical columns

YMC columns for SFC separation● 11 Selectivity Options● Quality Control via SFC evaluation● Below columns stocked for immediate

delivery

● 3 µm also available● Please inquire for preparative

versions and additional analytical configurations

Efficient separation of achiral APIs (Active Pharmaceutical Ingredients) in SFC modeInrecentyears,SFChasgainedenormouslyinimportanceandacceptanceinthepharmaceuticalindustry.Nowadays,itisroutinelyusedforthepurificationofenantiomers.Inaddition,SFChasassumedarelevantrolebothinanalyticalseparationsandlargerscalepurificationofachiralcompounds

asitisseenasaneconomicand"green"alternativetoclassicalHPLC.WehaveabundantpackingmaterialsforachiralSFCseparations.InabroadrangeofdifferentSFCselectivitythatcansolvethemostdiverseseparationproblems.

Packing material


Pore size（Å）


2-ethyl pyridine S-5 60 4.6×250 EP06S05-2546PS

Diol S-5 60 4.6×250 DP06S05-2546PS

120 4.6×250 DL12S05-2546PS

diethylaminopropyl S-5 60 4.6×250 DE06S05-2546PS

pyridine amide S-5 60 4.6×250 PY06S05-2546PS

propyl acetamide S-5 60 4.6×250 PP06S05-2546PS

Packing material


Pore size（Å）


silica S-5 120 4.6×250 SL12S05-2546PS

Polyamine II S-5 120 4.6×250 PB12S05-2546PS

amino S-5 120 4.6×250 NH12S05-2546PS

cyano S-5 120 4.6×250 CN12S05-2546PS

PVA-Sil S-5 120 4.6×250 PV12S05-2546PS

1

2

345

6

7

8

9

10

PDA-230 nmMIX500

450400350300250200150100500

0 1 2 3 4 5 6 7Minutes

8 9 10 11 12 13 14

mV

1. Citral2. Lidocaine3. Ibuprofen4. Caffeine5. Amitriptyline6. Propranolol7. Sulfinpyrazone8. Procainamide9. Cytosine

10. Sulpiride

A p p l i c a t i o n

Column ：YMC-PackPVA-Sil(5µm,120Å)250×4.6mmI.D.Flowrate ：3.5mL/minOutletpressure：15MPaColumntemp.：40℃Detection ：230nmInjectionvolume：5µLMobilephase：CO2withmodifierModifier ：Methanolw/0.1%DiethylamineGradientelution：linearchangefrominitial5%modifier-65%

modifierover12.5minutes

1

2

3

0

200

400

600

800

0 1 2 3Minutes

4 5 6 7

mA

U

Q u a l i t y c o n t r o l c h e c k b y S F C

Column ：YMC-PackDiol(5µm,120Å)250×4.6mmI.D.Mobilephase：80/20CO2/MethanolFlowrate ：2.0mL/minOutletpressure：10MPaColumntemp.：40℃Detection ：230nmInjectionvolume：5µL

QCmixcomponents1.Acetophenone2.Caffeine3.Thymine


第03章.indd 92 11.1.28 10:49:37 AM

Normal-Phase Columns · YMC Co., Ltd. offers normal-phase columns packed with bare silica gel or...

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Transcript of Normal-Phase Columns · YMC Co., Ltd. offers normal-phase columns packed with bare silica gel or...