Nordic-MUbio vet flyer update April 2015valterocchiena.com/pdf/nordic-mubio.pdf · antibodies for...

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Nordic MUbio

Visit www.nordicmubio.com/Products now to �nd the

products you need for your immunological research, or

get in touch on [email protected]

FIX&PERM® is a simple procedure making use of

two reagents. Reagent A gently �xes cells, while

Reagent B permeabilizes them.

The speci�c formulation of FIX&PERM reduces

background while allowing the simultaneous addition of

permeabilization medium and �uorochrome labelled

antibodies for staining of membrane proteins as well as

intracellular components, such as cytoplasmic enzymes,

nuclear proteins, oncoproteins, cytokines,

immunoglobulins, and so on.

• Mildly ixes cells, preserving their low cytometric scatter

characteristics

• Allows simultaneous characterisation of both

intracellular and cell surface markers

• Rapid technique – whole procedure can be carried out in

less than one hour, ready for immediate analysis or storage

for 24 hours

• Stringent QC procedures – the quality of each lot is

determined using well-de�ned blood samples and

subsequent comparison of immunostaining and scatter

characteristics of obtained leukocyte populations, ensuring

consistent and reliable results lot after lot

• A range of antibodies to intracellular and membrane

antigens with optimised protocols for use with FIX&PERM®

FIX&PERM®Cell Fixation and

Permeabilization Kit

Reagent A gently �xes cells preserving

their forward and side-scatter

characteristics

Reagent B permeabilizes the cell

Allowing binding and detection of

intracellular antigens

Reagent B and labelled antibodies are

then added

Contact your local distributor

here:


Nordic MUbio

The majority of available monoclonal antibody conjugates can be used with

FIX&PERM®.

However, in some cases the �xation step may require optimization.

Nordic-MUbio o�ers a number of mouse anti-human conjugated (PE/FITC)

monoclonal antibodies pre-optimised for use with FIX&PERM®.

FIX&PERM®Cell Fixation

and Permeabilization Kit

More products with protocols optimised for FIX&PERM can be found here:

http://www.nordicmubio.com/Services/FIXPERM

All FIX&PERM® kits are CE Marked

Procedure

For each sample to be analysed use

50 µl of whole blood, bone marrow

or mononuclear cell suspension in a

5 ml tube

Size Cat. Number

Fix&PERM® Kit 2 x 20 mL GAS-002

Fix&PERM® Sample Kit 2 x 5 mL GAS-002M

Fix&PERM® 1000 Kit 2 x 100 mL GAS-002-1

Fix&PERM® Reagent A (Fix) 1 x 100 mL GAS-002A-1

Fix&PERM® Reagent B (Perm) 1 x 100 mL GAS-002B-1

Add 100 µl of Reagent A (Fixation

Medium, stored and used at room

temperature)

Add 5 ml phosphate bu�ered saline

and centrifuge cells for 5 minutes at

300 g

Remove supernatant and add to cell

pellet 100 µl Reagent B and 20 µl of

the appropriate monoclonal antibody

Wash cells with PBS as above

Remove supernatant and resuspend

cells in sheath luid for immediate

analysis

Alternativly, resuspend cells in 0.5 ml

1.0 % formaldehyde and store at

2-8°C in the dark.

Analyse cells within 24 hours

Vortex at low speed for 1-2 seconds

Incubate for 15 minutes at room

temperature

Incubate for 15 minutes at room

temperature

Antigen Isotype Size Cat. Number

CD3 IgG1 0.2 mg GM-4011

100 tests GM-4012 (FITC)

100 tests GM-4013 (PE)

CD22 IgG1 0.2 mg GM-4051



Mouse anti-Human Combi Products

MPO-C2 IgG1 0.2 mg GM-4191



IgG Negative Control (FITC) and IgG Negative Control (PE) GIC-201

Anti-Lysozyme (FITC) and anti-Lactoferrin (PE) GIC-206

Anti-Myeloperoxidase-C2 (FITC) and anti-Lactoferrin (PE) GIC-212

Anti-Myeloperoxidase-C2 (FITC) and anti-CD22 (PE) GIC-214

Anti-Myeloperoxidase-C2 (FITC) and anti-CD3 (PE) GIC-213

Catalogue

NumberDescription Format

Unit

Size

Bii-nGFPab2-100Rabbit anti-GFP

(native)

Ammonium Sulfate

fraction0.1 mg


(native)Protein A Puriied 0.1 mg


(native)Ainity Puriied 0.1 mg

Bii-nGFPab4-PORabbit anti-GFP

(native):PO

Soyabean Peroxidase

Conjugate0.1 ml

Bii-nGFPabC1-100Chicken anti-GFP

(native)

Ammonium Sulfate

fraction0.1 mg

Bii-rGFP-100

Recombinant

Green Fluorescent

Protein (wild type)

Recombinant Protein 0.1 mg


Nordic MUbio

Green Fluorescent Protein

(GFP) is a 27kD protein derived

from the Aequorea victoria (A.

victoria) jellyish. It is a naturally

luorescent protein which has

revolutionised life science and

has been used to study proteins

in a wide range of applications.

GFP has been used extensively

to monitor gene expression; to

localise proteins at the cellular

and sub-cellular level; to track

metastatic cells and as a tag to

identify proteins in vitro.

The enormous interest in GFP

as a protein marker has given

rise to the development of

polyclonal and monoclonal

antibodies against GFP, used

for the immunolocalisation

and immunoblotting of GFP-

fused proteins in a variety of

applications.

Since its discovery, many variants

of GFP have been engineered

making antibodies recognising a

wide range of these variants an

important, and useful, scientiic

tool for use alongside GFP-fused

proteins.

A. victoria is a bioluminescent jellyish, or

hydromedusa, found of the west coast

of North America.

Nordic-MUbio is pleased to announce the release

of a new range of products directed against the

native form of GFP.

Our new range of anti-GFP antibodies bring a number of advantages

over antibodies produced against recombinant, or variant, forms of

GFP.

This includes the ability to detect GFP produced from a wide range

of diferent sources, including native, recombinant, and a number of

diferent mutants, without sufering from high levels of non-speciic

binding/background.

Part of the reason many anti-recombinant GFP antibodies sufer from

high background issues is due to contamination of an innoculum

with other E.coli components, leading to increased non-speciic

binding.

Our products were tested for cross-reactivity to a wide range of

antigens, including many derived from E.coli, and demonstrated no

cross-reactivity.

High Quality Green Fluorescent

Protein (GFP) Antibodies


Nordic MUbio

Anti-GFP

Ab’ source

HPLC

Purity

SDS

Purity

Western

Speciicity

Native GFP

AntibodyClean Clean Clean

Competitor A Contaminants ContaminantsSome

Background

Competitor B Clean CleanHigh

Background

Competitor C Multiple peaksSlight

Contaminantsn/a

Competitor D Many peaks

(contains BSA)Not run n/a

Competitor E Multiple peaks Contaminants n/a

Competitor F Multiple peaks Contaminants n/a

Competitor G Multiple peaks Clean n/a

Example Size Exclusion

Chromatography (HPLC) Results

Competitor D

Bii-nGFPab3-100

(Protein A puriied)

Competitor E

Western blot Anlaysis

The gels in these images have been loaded

with E.coli control, not expressing GFP

(lane A), E.coli expressing GFP (lane B), and

molecular weight standards (lane C).

Competitor A Competitor B Nordic-MUbio

Native GFP

antibody

These images show intact GFP (main band

at 27 kDa), with some high and low mol.

weight bands related to GFP present in

all. The antisera from competitors A and B

show more background reactivity in the E.

coli control (lane A).

To ensure Nordic-MUbio’s new GFP antibody range is of the highest

quality a number of comparison experiments were run with the best-

selling antibodies from other suppliers against recombinant GFP,

assessing purity, speciicity, and titre of the antibodies.

This page presents a number of the indings from these anlayses.

Visit www.nordicmubio.com/Products now to ind the



The HPLC analyses of the Nordic-

MUbio ainity puriied antibodies

show just a single peak, illustrating

their purity. The competitor

antibodies with multiple peaks

have contaminants, such as serum

albumin, which can afect the results

of e.g. Western blots or ELISAs.

Bii-nGFPab3-100

(Protein A puriied)

How do we know our GFP products are

the highest quality?

Isotyping in research

In vaccine research, the isotype of the antibodies produced post-vaccination can give information on the type of immune response induced. For example, stronger induction of an IgA response might be desirable for a vaccine targeting viral pathogens, induc-ing mucosal immunity. While higher levels of IgG2 are corre-lated with a Th1 (pro-in�ammatory) response, and higher IgG1 is more characteristic of a Th2 (anti-in�ammatory) response.

Screening for antibodies devel-oped against biologic drugs is a requirement of most clinical trials and post-approval monitoring. When a patient does develop an immune response it can be useful to determine both the speci�city and antibody isotype of the response.


Nordic MUbio

Nordic-MUbio o�ers the highest quality

range of products for the study and

analysis of human immunoglobulins.

Our highly validated product range spans mouse monoclonal

antibodies against all isotypes of human immunoglobulins,

including allotypes, and are available in multiple conjugates

suitable for applications such as ELISA, �ow cytometry, western

blot and immunohistochemistry.

We also have a range of highly puri�ed human immunoglobulins,

suitable for use as references and antigens.

Determining the class (isotype) and subclass of an antibody is

important for a variety of reasons, from selection of puri�cation

protocols to day-to-day immunological methods and clinical

diagnostics.

Di�erent antibody classes and subclasses serve a variety of di�erent

functions within the body and can be indicative of disease states

and immunological processes. For example, IgE is characteristic of

allergic reactions. Each antibody isotype has unique e�ector

functions, capable of binding di�erent cellular receptors.

Visit nordicmubio.com/Products now to �nd the answer to all

your human immunoglobulin needs, or get in touch on

[email protected]

Human Immunoglobulins


here:


Nordic MUbio

Nordic-MUbio has developed an

ever-expanding range of high

quality immunological research

tools, manufactured to strict ISO

9001 guidelines, to provide both

reassurance and the best possible

tools for your research.

Visit nordicmubio.com/Products

now to �nd the answer to all your

human immunoglobulin needs,

or contact us:

[email protected]

Puri�ed Human

ImmunoglobulinCat. Number

IgG HuIgG

IgG subclasses standard Serum NOR-01

IgG1 lambda G1m(f) P 106

IgG4 kappa Gm4a P 182

IgG1 lambda G1m (a) P 210

IgG3 lambda G3m (g) P 289

IgG4 kappa Gm4b P 304

IgG2 lambda G2m n-) P 315

IgG3 kappa G3m(b) P 411

IgG2 lambda G2m (n+) P 532

IgA subclasses standard Serum NOR-04

IgA1 lambda (dimer) P 444

Secretory IgA P 020

IgA1 kappa, polymer P 430

IgA2 lambda A2m (1-, 2+) P 505

IgA2 (1+,2-) lambda P 237

Human IgE kappa P 050

Human Bence Jones kappa

protein (Free light chain, kappa type)

P 016

Human Bence Jones lambda

protein (Free light chain, lambda type)

P017

Human Immunoglobulin Products

Mouse anti-human Cat. Number

IgG1 MAHu/IgG1 B,H,F,T

IgG2 (Fab subclass speci�c) MAHu/IgG2 B,H,F,T

IgG2 (Fc subclass speci�c) MAHu/IgG2Fc B,H,F,T

MAHu/IgG3 B,H,F,T

MAHu/IgG3m(u) B,H,F,T

IgG4 (pFc subclass speci�c) MAHu/IgG4 B,H,F,T

IgG (class speci�c) MAHu/IgGc B,H,F,T

IgM (class speci�c) MAHu/IgM B,H,F,T

IgA1 MAHu/IgA1 B,H,F,T

IgA2 MAHu/IgA2 B,H,F,T

IgA2 (m2 allotype) MAHu/IgA2(m)2 B,H,F,T

IgA1 + IgA2 MAHu/IgAc B,H,F,T

IgE MAHu/IgE B,H,F,T

IgD MAHu/IgD B,H,F,T

*B= Biotin, H=Horseradish peroxidase, F = FITC, T=TRITC

AvailableConjugates*

IgG3 (F(ab'')2 subclass speci�c)

IgG3 (G3m(U) allotype speci�c)


Nordic MUbio

Visit www.nordicmubio.com/Products now to �nd the



Nordic-MUbio has developed an

ever-expanding range of high quality

immunological research tools,

manufactured to strict ISO 9001

guidelines, to provide both

reassurance and the best possible

tools for your research.

Veterinary Immunoglobulins

Nordic-MUbio is dedicated to helping veterinary

scientists advance their understanding of animal

immunology, helping to safeguard everything

from domestic pets to livestock in health and

disease.

Our highly validated veterinary immunoglobulin products

include a wide range of both host and target species, giving

researchers the scope and choice they need.

As in humans, animal immunoglobulins play a huge role in

health and disease, with di�erent antibody classes and

subclasses indicative of di�erent disease states or normal

activity of immunological processes.

This makes animal immunoglobulins of interest and

importance to a huge range of research areas from infectious

diseases to cancer research.

We know your research needs reliable products that just work.

And that’s why we’ve developed a portfolio of veterinary

immunoglobulin products with a wealth of validation data,

suitable for use as either primary or secondary antibodies and

in a range of formats to suit your research needs.

Whether your application is immunoprecipitation, ELISA or

immunohistochemistry, if we list it as an application you can

be sure that our products will work. Saving you not only

money, but more importantly, time and precious sample.

Together with our strict ISO 9001 manufacturing and quality

control you can be sure each of our products will work time

after time, lot after lot.


here:

Our 15 Top Selling Veterinary Immunoglobulin Products


Nordic MUbio

Conjugate Key:B: Biotin

H: Horseradish peroxidase

F: FITC

T: TRITC

Applications Key:EL: ELISA

ICC: Immunocytochemistry

IHC(F): Immunohistochemistry (frozen)

IHC(P): Immunohistochemistry (parra�n)

DB: Dot blotting

IB: Immunoblotting

IP: Immunoprecipitation

IF: (in)direct immuno�uorescence

Our complete range of veterinary

products includes reagents for research

into: Cow, Dog, Chicken, Duck, Electric

Eel, Cat, Goat, Guinea Pig, Hamster,

Horse, Mouse, Rabbit, Rat, Sheep, Snake,

Swine and many more.

Anti IgG*

Host Anti- Cat. Number Conjugate Application

Anti IgG1, IgG2a, IgG2b, IgG3, IgA, IgM*


Anti IgG1, IgG2a, IgG2b, IgG3*


Anti serum proteins


Rabbit Goat RAG/IgG(H+L) B, F, H, T EL, ICC, IHC(P), DB, IB, IP

Swine Mouse SwAM/IgG(H+L) F EL, ICC, IHC(F), IF

Rabbit Swine RASw/IgG(H+L) B, F, H, T EL, ICC, IHC(P), DB, IB, IP, IF

Rabbit Dog RAD/IgG(H+L) B, F, H, T EL, ICC, IHC(P), DB, IB, IP

Goat Cow GAB/IgG(H+L) B, F, H EL, ICC, IHC(P), IHC(F), DB, IB, IP

Goat Rabbit GAR/IgG(H+L) B, F, H, T

B, F, H, T

EL, ICC, IHC(P), IHC(F), DB, IB, IP

Rabbit Horse RAHo/IgG(H+L) B, F, H EL, ICC, IHC(P), IHC(F), DB, IB, IP

Rabbit Cat RACa/IgG(H+L) B, F, H EL, ICC, IHC(F), DB, IB, IP, IF

Rabbit Duck RADu/IgG(H+L) B, F, H EL, ICC, IHC(P), IHC(F), DB, IB, IP

Rabbit Chicken RACh/IgG(H+L) B, F, H EL, ICC, IHC(P), DB, IB, IP, IF

Anti IgD (Fc speci�c)


Goat Mouse GAM/IgD(Fc) EL, ICC, IHC(F), IP, IF

B, F, H, TRabbit Mouse RAM/Ig EL, ICC, IHC(P), IHC(F), DB, IB, IP

B, F, H, TGoat Mouse GAM/IgG(H+L) EL, ICC, IHC(P), DB, IB, IP

_Rabbit Horse RAHo/TSP IP

Anti Beta lactoglobulin


_Rabbit Cow RAB/BLgl IP

*Heavy and Light chains

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