Non Transgenic Replacement and Suppression of …...3/21/2016 1 Non‐Transgenic Replacement and...
Transcript of Non Transgenic Replacement and Suppression of …...3/21/2016 1 Non‐Transgenic Replacement and...
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Non‐Transgenic Replacement and Suppression of Vector Mosquitoes
Stephen Dobson
13 March, 2016
Summit on the Aedes aegypti Crisis in the Americas: Joining Together to Address a Grand Challenge
Maceió, Brazil
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Classical Sterile Insect Technique via Irradiation… several success stories in agriculture.
• Screw Worm Fly• Mediteranean Fruit Fly• Pink Boll Worm
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Classical Sterile Insect Technique via Irradiation
… but hasn’t worked well with mosquitoes
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Bidirectional IncompatibilityBidirectional Incompatibility
Aedes aegypti
Scott O’Neill
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Wolbachia‐basedStrategies
• Population Replacement• Release of Wolbachia infected females
• Goal: NOT intended as a pesticide; no reduction of the Ae. aegypti population
• Goal: infect local populations of mosquitoes with Wolbachia, which is intended to interfere with virus transmission
• Wolbachia Suppression
• Release of Wolbachia infected males
• Goal: reduce mosquito densities with sterile male releases (similar to insecticides)
• Aedes albopictus
• Aedes aegypti
Wolbachia‐basedStrategies
• Population Replacement• Release of Wolbachia infected females
• Goal: NOT intended as a pesticide; no reduction of the Ae. aegypti population
• Goal: infect local populations of mosquitoes with Wolbachia, which is intended to interfere with virus transmission
• Wolbachia Suppression
• Release of Wolbachia infected males
• Goal: reduce mosquito densities with sterile male releases (similar to insecticides)
• Aedes albopictus
• Aedes aegypti
Self‐sustainingInterference: Fitness Costs
Zika? other viruses?Potential virus selection
Not self‐sustaining(unless achieve elimination)
Successful examples of SIT
Wolbachia does not negatively effect male fitness
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http://www.epa.gov/oppbppd1/biopesticides
Regulatory Consideration – Wolbachia Microbial Pesticide
Lexington, KY
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Los Angeles,
CA
Fire Island, NY
Lexington, KY
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Auto-Dissemination by Autocidal Males (ADAM)
Hormone Treatment
• Effects conspecifics in the same breeding sites• Interspecific and Intraspecific effect
Clovis, CA
Lexington, KY
Aedes albopictus
Aedes aegypti
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Lexington, Kentucky
Robert ‘Bob’ Rose, PhD
Kentucky Cabinet for Economic Development