NobuyukiMatobaCV

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NobuyukiMatoba,Ph.D.

ClinicalandTranslationalResearchBuilding,Room615505S.HancockStreetLouisville,KY40202

OfficePhone:(502)8528412Fax:(502)8525468

E-mail:n.matoba@louisville.eduEDUCATION

1992–1996 B.Sc.inFoodBioscienceandTechnology,FacultyofAgriculture,KyotoUniversity,

Japan1996–1998 M.Sc.inAppliedLifeScience,GraduateSchoolofAgriculture,KyotoUniversity,

Japan(Advisor:MasaakiYoshikawa,Ph.D.)1998–2001 Ph.D.(AppliedLifeScience),GraduateSchoolofAgriculture,KyotoUniversity,

Japan(Advisor:MasaakiYoshikawa,Ph.D.)ACADEMICAPPOINTMENTS1/2006–9/2008 ResearchAssistantProfessor

CenterforInfectiousDiseases&Vaccinology,BiodesignInstitute,ArizonaStateUniversity,Tempe,AZ

10/2008–12/2013 AssistantProfessor(tenuretrack)DepartmentofPharmacologyandToxicology,UniversityofLouisvilleSchoolofMedicine,Louisville,KY

1/2014–present AssociateProfessor(withtenure)DepartmentofPharmacologyandToxicology,UniversityofLouisvilleSchoolofMedicine,Louisville,KY

10/2008–present ScientistJamesGrahamBrownCancerCenter,UniversityofLouisvilleSchoolofMedicine,Louisville,KY

OTHERPOSITIONSANDEMPLOYMENT2001 PostdoctoralAssociate

ResearchInstituteforFoodScience,KyotoUniversity(Advisor:MasaakiYoshikawa,Ph.D.)

2001–2002 VisitingScientist DepartmentofPlantBiology,ArizonaStateUniversity(Sponsor:TsafrirMor,Ph.D.)

2002–2004 PostdoctoralResearchAssociateDepartmentofPlantBiology,ArizonaStateUniversity(Advisor:TsafrirMor,Ph.D.)

2004–2005 FacultyResearchAssociate

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CenterforInfectiousDiseases&Vaccinology,BiodesignInstituteandSchoolofLifeSciences,ArizonaStateUniversity,Tempe,AZ(Advisors:CharlesArntzen,Ph.D.andTsafrirMor,Ph.D.)

CERTIFICATIONANDLICENSURENotapplicable

PROFESSIONALMEMBERSHIPSANDACTIVITIES2008–present MemberoftheSocietyforMucosalImmunology2010–present MemberofKentuckyAcademyofScience2013–present MemberofAmericanSocietyforMicrobiology2014–present MemberoftheProteinSocietyHONORSANDAWARDS

1996–1999 GraduateScholarship,JapanScholarshipFoundation2000–2002 ResearchFellowshipforYoungScientists,JapanSocietyforthePromotionof

Science2004–2006 PostdoctoralFellowshipforResearchAbroad,JapanSocietyforthePromotionof

Science03/2005 TravelAward,KeystoneSymposiumonHIVVaccines:CurrentChallengesand

FutureProspects06/2005 YoungInvestigatorAward,AnnualmeetingoftheSocietyforMucosal

Immunology03/2008 ScholarshipAward,KeystoneSymposiaFellowsandNewInvestigatorsWorkshop

onGrantsmanshipandCareerResearchOpportunitiesinHIV/AIDSResearch11/2009 RogerH.HerzigJuniorFacultyResearchPrize(3rdplace),JamesGrahamBrown

CancerCenter8thAnnualRetreat05/2010 ScholarshipAward,2010InternationalMicrobicideConference05/2014 2014ScientistoftheYear,JamesGrahamBrownCancerCenter,Universityof

LouisvilleCOMMITTEEASSIGNMENTSANDADMINISTRATIVESERVICESServicetotheDepartment

2009–2014 Member,EventCommittee,DepartmentofPharmacology&Toxicology,UniversityofLouisvilleSchoolofMedicine

2009–2014 Organizer,JournalClubatOwensboroCancerResearchProgram2011–2015 OwensboroCancerResearchProgramFacultySearchCommittee

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2014–present Chair,SIBUP(salaryincreasesbaseduponperformance)/GrievanceCommittee,DepartmentofPharmacology&Toxicology,UniversityofLouisvilleSchoolofMedicine

ServicetotheUniversity

2009–2013,2017 Judge,Research!Louisville2014–present Member,InstitutionalBiosafetyCommittee,UniversityofLouisvilleServicetothecommunity,State,Nationorinternationalorganizations

2010 Judge,BiochemistrysectionofLouisvilleRegionalScienceFairs2009–present AssociateFacultyMember,Facultyof1000Biology2011 Grantreviewer,NationalResearchFoundation,theRepublicofSouthAfrica2013 Grantreviewer,theNazarbayevUniversityResearchCouncilinAstana,

Kazakhstan2015 Grantreviewer,NIH/NIAIDSpecialEmphasisReviewPanel(ZAI1-JBS-A-M1),

SustainedReleaseforAntiretroviralTreatmentorPrevention(SRATP)ofHIVInfection(UM1)

2016 Grantreviewer,NIH/NIAIDSpecialEmphasisReviewPanel(ZRG1AARR-M54),PreventionInnovationProgramIII(R01)

2017 Grantreviewer,NaturalSciencesAndEngineeringResearchCouncil(NSERC)CollaborativeResearchandDevelopment(CRD)GrantProgram,Canada

EDUCATIONALACTIVITIESCoursesorganizedorinstructed

2006–2008 Guestinstructor,MCB500Strategiesforproductionofrecombinantprotein-pharmaceuticals,ArizonaStateUniversitySchoolofLifeSciences

2010–2015 Organizer,JournalClubsessionforsummerresearchinternstudentsatOwensboroCancerResearchProgram,OwensboroMedicalHealthSystemandUniversityofLouisvilleSchoolofMedicine

2010–2016 Guestinstructor,PHTX675ResearchMethodsinPharmacologyandToxicology,UniversityofLouisvilleSchoolofMedicine(GeneticScreeningandPhageDisplayforNewAntibodyTherapeutics)

2017 Guestinstructor,PHTX642GraduatePharmacologyIISpring2016(AntiviralandAntiretroviralAgents)

2017 GuestinstructorofPHTX641GraduatePharmacologyI,Fall2017(AntiviralDrugs)

2017 GuestinstructorofBMSC807-05_4178PharmacologyandDentalTherapeutics,Fall2017(DrugsActingontheGastrointestinalTract)

Lecturesinformalcoursesandinvitedseminars

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06/2004 Guestlectureon“Heat-stable,OralVaccinesFromTransgenicPlants”AdvancedBiotechnologyCourse(Graduatelevel,Bioengineering),OsakaUniversity

3/2005 Guestlectureon“ConstructingamucosalsubunitvaccinetopreventHIV-1transmissionandinfection”MCB701MolecularCellularBiologyColloquium,ArizonaStateUniversity

12/2007 Guestlectureon“PlantBiotechnologyforHumanHealth”SpeciallectureseriesintheFoodScienceandBiotechnologymajor,KyotoUniversityGraduateSchoolofAgriculture

06/2009 Guestlectureon“BioProductionofRecombinantProteinPharmaceuticalsinPlants”FacultyofPharmacyUndergraduateCourse,IwakiMeiseiUniversity

10/2009 Guestlectureon“ProductionofRecombinantProteinPharmaceuticalsinPlants”BiotechnologyCourse,DepartmentofBiology,WesternKentuckyUniversity

10/2009 Guestlectureon“PlantBiotechnologyforHumanHealth:ProductionofRecombinantProteinPharmaceuticalsinPlants”KentuckyWesleyanCollegeGeneticsCourse(undergraduatelevel)

06/2013 Guestlectureon“DevelopmentofProteinPharmaceuticalsBasedonMolecularFarming”FacultyofAgriculture,TheUniversityofTokyo

06/2013 Guestlectureon“MolecularFarmingofBiopharmaceuticals”FacultyofPharmacyUndergraduateCourse,IwakiMeiseiUniversity

06/2013 Guestlectureon“MolecularFarmingofBiopharmaceuticals”FacultyofAgriculture,KyotoUniversity

07/2013 Guestlectureon“MolecularFarmingofRecombinantProteinPharmaceuticals”FacultyofEngineering,OsakaUniversity

07/2015 Invitedseminaron“Therapeutic effects of a cholera toxin vaccine antigen produced in Nicotiana benthamiana plants”Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan

07/2015 Guestlectureon“Molecular farming of protein pharmaceuticals” Tokyo University of Agriculture, Tokyo, Japan

07/2015 Invitedseminaron“Molecular Farming of Protein Pharmaceuticals” Kanazawa University Medical School, Kanazawa, Japan

11/2015 Invitedseminaron“Engineering of a lectibody targeting viral- and tumor-associated high-mannose glycans” Department of Chemical Engineering and Materials Science, University of California Davis

2/2016 Invitedseminar“Oraladministrationofacholeravaccineantigeninducesmucosalhealinginthecolon”PriceInstituteforSurgicalResearch,DepartmentofSurgery,UniversityofLouisvilleSchoolofMedicine

3/2016 Invitedseminar“Anoligomannose-specificlectin-FcfusionproteinagainstHIVinfection”DepartmentofMicrobiologyandImmunology,UniversityofLouisvilleSchoolofMedicine

4/2016 “Plantviralvector-basedtransientexpressionforrapidscreeningandcharacterizationofnovelproteinpharmaceuticals”ThePhyto-EngineeringResearchCenterworkshop,UniversityofCaliforniaDavis

3/2017 Invitedseminar,“Plant-madebiopharmaceuticals”DepartmentofBioengineeringSpring2017seminarseries,UniversityofLouisville

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11/2017 Invitedseminar“ThecolonepithelialhealingactivityofEpicertin,acholeratoxinBsubunitvariant”PriceInstituteforSurgicalResearch,DepartmentofSurgery,UniversityofLouisvilleSchoolofMedicine,November9,2017.

Ph.D.andMasterthesiscommittee

2006–2008 CommitteeMemberforMs.NamrataShah,M.S.studentofMolecularCellularBiology,SchoolofLifeSciences,ArizonaStateUniversity

2006–2012 CommitteeMemberforMs.SarahKessans,Ph.D.studentofMolecularCellularBiology,SchoolofLifeSciences,ArizonaStateUniversity

2009–2014 CommitteeMemberforMr.ChristopherBarton,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

01/2012–12/2015 CommitteeChairforMr.KeeganBaldauf,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

12/2012–05/2016 CommitteeMemberforMs.AmandaLasnik,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

01/2016–present CommitteeChairforMr.JoshuaRoyal,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

07/2016–present CommitteeMemberforMr.KevinTyo,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

08/2016–present CommitteeChairforMr.MatthewDent,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

Advisorforpostdoctoralfellows,Ph.D./Masterstudents

10/2010–12/2010 Co-advisor(withDr.Palmer)forMs.HuihuiWuduringherlabrotationoffirst-yearPh.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

08/2010–02/2012 Co-advisor(withDr.Palmer)forJosephCalvinKoukam,Ph.D.,PostdoctoralScholar

09/2010–03/2014 MajorAdvisorforKrystalTeasleyHamorsky,Ph.D.,PostdoctoralResearchAssociate(PromotedtoInstructorofMedicineatUniversityofLouisville)

01/2012–09/2015 MajorAdvisorforTiffanyGrooms-Williams,Ph.D.,PostdoctoralResearchAssociate

08/2012–02/2013 MajorAdvisorforMs.AprilAlloway,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

01/2012–12/2015 MajorAdvisorforMr.KeeganBaldauf,Ph.D.studentofPharmacologyandToxicologyatUniversityofLouisvilleSchoolofMedicine

02/2015–10/2016 MajorAdvisorforSugataRoyChowdhury,Ph.D.,PostdoctoralResearchAssociate08/2016–present MajorAdvisorforMr.MatthewDent,M.S.,PhDstudentofPharmacologyand

Toxicology,UniversityofLouisvilleSchoolofMedicine09/2016–present MajorAdvisorforYoungjunOh,Ph.D.,VisitingScientist/PostdoctoralAssociateMentoringofundergraduateandhigh-schoolstudents

03/2005–05/2007 Mr.TaganGriffin,ArizonaStateUniversity

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04/2006–08/2007 Mr.HowardCheng,CoronadelSolHighSchool04/2006–10/2007 Mr.JonathanHu,CoronadelSolHighSchool05/2007–08/2008 Ms.MichellePickel,ArizonaStateUniversity11/2007–05/2008 Ms.JenniferHurt,ArizonaStateUniversity05/2008–08/2008 Ms.PriyaRaman,YaleUniversity05/2008–09/2008 Ms.HarmanDhatt,ArizonaStateUniversity02/2009–08/2009 Ms.HillaryConway,KentuckyWesleyanCollege06/2009–08/2009 Mr.DavidGrunewald,SaintLouisUniversity09/2009–12/2009 Ms.NancyPettiborne,OwensboroCommunity&TechnicalCollegeandWestern

KentuckyUniversity12/2009–07/2010 Ms.BrittneyMetts,KentuckyWesleyanCollege03/2010–08/2010 Mr.AndrewDonovan,KentuckyWesleyanCollege06/2010–08/2010 Mr.DavidGrunewald,SaintLouisUniversity07/2010–07/2011 Ms.AshleyHumphrey,KentuckyWesleyanCollege05/2011–08/2011 Ms.NatoshaMulholland,WesternKentuckyUniversity05/2011–08/2011 Mr.SamuelAsante,LindseyWilsonCollege,KY02/2011–05/2012 Ms.LaurenBennett,KentuckyWesleyanCollege05/2012–08/2012 Ms.BaileyNelson,CentreCollege,KY05/2012–08/2013 Mr.DanielSpeer,KentuckyWesleyanCollege,KY06/2014–08/2014 Mr.LukeRoyal,LindseyWilsonCollege,KY06/2015–07/2015 Mr.JeremiahWayne,TheGattonAcademy,KY06/2015–07/2015 Mr.GrahamReynolds,TheGattonAcademy,KY09/2015–06/2016 Mr.NehalNinad,UniversityofSouthernIndiana,IN05/2017–present Mr.DavidMorris,UniversityofLouisville,KY06/2018–08/2018 Mr.ChanduKona,NorthOldhamHighSchool,KYCLINICALACTIVITIESNotapplicableGRANTSANDCONTRACTSActiveGrants/Contracts

1. FundingAgency: NIHGrantNumber: U19AI113182CoreC(Matoba)FundingAmount: $2,138,020(directcosts)and$362,683(F&A)Duration: 7/01/14–6/30/19ProjectTitle: Griffithsin-basedRectalMicrobicidesforPREventionofViralENTry(PREVENT)

PharmacokineticsandPharmacodynamicsCoreRole: PI,25%effort

2. FundingAgency: NIH

GrantNumber: 1R21CA216447-01A1(Matoba)

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FundingAmount: $239,250(directcosts)and$136,986(F&A)Duration: 2/08/18–1/31/20 ProjectTitle: Investigationofalectibodytargetingtumor-associatedoligomannoseglycansRole: PI,15%effort

3. FundingAgency: NIH

GrantNumber: U10HL127518-01(Bates/Miller/Krentsel;co-PIs)FundingAmount: $200,000(subprojectdirectcosts)Duration: 2/01/16–1/31/19 ProjectTitle: TheEXCITEProgram:ExpeditingCommercialization,Innovation,Translation

andEntrepreneurshipPilotProjectTitle: OralsoliddosageformulationofcholeratoxinBsubunit.Role: Subprojectco-PI(withHamorsky),5%effort

4. FundingAgency: NIHGrantNumber: U10HL127518-01(Bates/Miller/Krentsel;co-PIs)FundingAmount: $200,000(subprojectdirectcosts)Duration: 3/01/17–2/28/19 ProjectTitle: TheEXCITEProgram:ExpeditingCommercialization,Innovation,Translation

andEntrepreneurshipPilotProjectTitle: Avaren-FclectibodyforlivergraftprotectionagainsthepatitisCvirusinfectionRole: SubprojectPI(withHamorsky),5%effort

5. FundingAgency: NIH

GrantNumber: U10HL127518-01(Bates/Miller/Krentsel;co-PIs)FundingAmount: $100,000(subprojectdirectcosts)Duration: 9/01/17–8/31/18 ProjectTitle: TheEXCITEProgram:ExpeditingCommercialization,Innovation,Translation

andEntrepreneurshipPilotProjectTitle: CTBpfororalmucositistreatmentRole: SubprojectPI(withHamorsky),5%effort

6. FundingAgency: NIH

GrantNumber: T32ES011564(DavidHein)FundingAmount: $2,037,745(total)Duration: 7/01/16–6/30/21 ProjectTitle: UofLEnvironmentalHealthSciencesTrainingProgram.Role: Facultymentor(nosalarysupport)

7. FundingAgency: NIHGrantNumber: R25-CA134283(DavidW.Hein;LaCreisR.Kidd)FundingAmount: $1,593,000(total)Duration: 04/01/17–03/31/22 ProjectTitle: UniversityofLouisvilleCancerEducationProgram.

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Role: Facultymentor(nosalarysupport)CompletedGrants/Contracts

1. FundingAgency: JapanSocietyforthePromotionofScienceProgram: ResearchGrantforJSPSResearchFellow(Matoba)FundingAmount: 2,000,000JPY(directcosts)Duration: 1/1/2000–3/31/2002ProjectTitle: DevelopmentofFoodCropsPossessingAnti-HypertensiveActivityRole: PI,100%effort

2. FundingAgency: JapanSocietyforthePromotionofScience

Program: ResearchGrantforJSPSPostdoctoralFellow(Matoba)FundingAmount: 1,040,000JPY(directcosts)Duration: 4/1/2004–3/31/2006 ProjectTitle: ConstructionofPlant-BasedMucosalVaccinesAgainstHIVRole: PI,100%effortFundingAgency: NIH/NIAID

3. GrantNumber: R03AI073157(Matoba)FundingAmount: $100,000(directcosts)Duration: 3/15/2007–2/28/2010ProjectTitle: ExpressionofDeconstructedHIV-1Virus-LikeParticlesinBioengineeredPlantsRole: PI,50%effort(3/15/2007–12/31/2008);and10%effort(1/1/2009–

2/28/2010)

4. FundingAgency: UniversityofLouisvilleSchoolofMedicineGrantNumber: BasicGrantProgramE0581(Matoba)FundingAmount: $15,000(directcosts)Duration: 4/1/2009–3/31/2010ProjectTitle: ProductionandEvaluationofPlant-madeAnti-HIVProteinActinohivinRole: PI,20%effort(nosalarysupport)

5. FundingAgency: UniversityofLouisvilleGrantNumber: ClinicalandTranslationalSciencePilotGrantProgramAward-20002(Matoba)FundingAmount: $50,000(directcosts)Duration: 3/1/2010–2/28/2011ProjectTitle: Abroad-spectrumvaccineagainstenvelopedvirusesRole: PI,20%effort(nosalarysupport)

6. FundingAgency: UniversityofLouisvilleOfficeoftheVicePresidentforResearchGrantNumber: IRIGResearchInitiationGrant50721(Matoba) FundingAmount: $5,000 (directcosts)Duration: 06/01/11–11/30/12

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ProjectTitle: Prophylacticpotentialofplant-producedcholeratoxinBsubunitsinexperimentalcolitis.

Role: PI,10%effort(nosalarysupport)

7. FundingAgency: UniversityofLouisvilleOfficeoftheVicePresidentforResearchGrantNumber: IRIGCompetitiveEnhancementGrant50730(Matoba)FundingAmount: $15,000(directcosts)Duration: 10/01/11–12/31/12ProjectTitle: Developmentofarecombinantentry/fusion-bispecificinhibitortowarda

topicalHIV-1microbicide. Role: PI,10%effort(nosalarysupport)

8. FundingAgency: DoD/USMRAA/TATRCGrantNumber: W81XWH-09-2-0022(DonaldC.WilkersonatOMHS)FundingAmount: $1,680,000(totalcosts)Duration: 03/15/09–03/14/13ProjectTitle: DevelopmentofNovelVaccinesandTherapeuticsUsingPlant-BasedExpression

SystemsRole: Sub-projectPI,10%effort

9. FundingAgency: DoD/USMRAA/TATRCGrantNumber: W81XWH-10-2-0082-CLIN1(DonaldC.WilkersonatOMHS)FundingAmount: $1,751,000(total)Duration: 8/23/2010–9/22/2013ProjectTitle: Plant-BasedExpressionSystemsforNewVaccinesandTherapeuticsRole: Sub-projectPI,10%effort

10. FundingAgency: NIHGrantNumber: R21/R33AI088585(Matoba)FundingAmount: $1,175,000(directcosts)Duration: 6/10/2010–6/31/2016ProjectTitle: Plant-producedActinohivinasaCandidateHIVMicrobicideRole: PI,30%effort

11. FundingAgency: DoD/USMRAA/TATRC

GrantNumber: W81XWH-10-2-0082-CLIN2(Wilkerson)FundingAmount: $1,748,000(total)Duration: 9/30/2011–10/31/2016 ProjectTitle: Plant-BasedExpressionSystemsforNewVaccinesandTherapeuticsRole: SubprojectPI,5%effort

12. FundingAgency: NIHGrantNumber: T32ES011564(DavidW.Hein)FundingAmount: $2,037,745(total)

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Duration: 7/01/09–6/30/14 ProjectTitle: UofLEnvironmentalHealthSciencesTrainingProgram.Role: Facultymentor(nosalarysupport)

13. FundingAgency: NIHGrantNumber: R25-CA134283(DavidW.Hein)FundingAmount: $1,543,610(total)Duration: 09/14/11–08/30/16 ProjectTitle: UniversityofLouisvilleCancerEducationProgram.Role: Facultymentor(nosalarysupport)

14. FundingAgency: NIHGrantNumber: P30GM106396(Miller)FundingAmount: $150,000(pilotprojectdirectcosts)Duration: 7/01/15–6/30/17 ProjectTitle: MolecularTargetsPhaseIIICoBREPilotProjectTitle: Plant-madelectibodytargetingtumor-associatedhigh-mannose-glycan

antigensasanovelcancerimmunotherapeutic/diagnosticagent.Role: PilotprojectPI,5%effort

15. FundingAgency: HelmsleyCharitableTrustGrantNumber: 2014-PG-MED001(Miller)FundingAmount: $225,000(subprojectdirectcosts)Duration: 11/01/15–6/30/18ProjectTitle: Advancingthediscoveryanddevelopmentofplant-madepharmaceuticals SubProjectTitle: Plant-madeN-mannosylatedcholeratoxinBsubunitasanovelvaccinescaffold.Role: SubprojectPI,5%effort

PATENTSAwarded

1. US7438914B2(AwardDate:10/21/2008)Title:CompositionandMethodforEnhancingImmuneResponse

2. US7927833B2(AwardDate:4/19/2011)Title:CompositionandMethodforEnhancingImmuneResponse

1. US8802822B2(AwardDate:8/12/2014)Title:Polypeptideshavingantiviralactivityandmethodsforusethereof

2. US9133252B2(AwardDate:9/15/2015)Title:Polypeptideshavingantiviralactivityandmethodsforusethereof

Applications

1. ApplicationDate:March14,2012

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ApplicationNumber:PCT/US2012/029072Title:Polypeptideshavingimmunoactivatingactivityandmethodsofproducingthesame

2. ApplicationDate:February11,2014ApplicationNumber.PCT/US2014/015861Title:Methodsforproducingantibodies

3. ApplicationDate:July16,2014ApplicationNumber.PCT/US2014/046893Title:Compositionsformucosaldelivery,usefulfortreatingpapillomavirusinfections

4. ApplicationDate:Jun29,2016ApplicationNumber.PCT/US2016/040041Title:Compositionsandmethodsfortreatingcancerandpromotingwoundhealing

EDITORIALWORKJournalEditorialBoards,AdvisoryCouncils,PeerReviewerofManuscripts

09/2009–present AssociateFaculty,Facultyof1000BiologyAgricultureandBiotechnologysection11/2004–present Adhocmanuscriptreviewerfor: BiotechnologyJournal;CellularandMolecular

LifeScience;CurrentMedicinalChemistry;ExpertReviewofVaccine;FASEBJournal;HortScience;JournalofAntimicrobialChemotherapy;JournalofBioscienceandBioengineering;JournalofVisualizedExperiments;MolecularBiologyReport;MolecularBiotechnology;NatureBiotechnology;PlantBiotechnologyJournal;PLoSONE;PNAS;ProcessBiochemistry;Vaccine

6/2015–present EditorialBoardmember,ScientificReports7/2017–present AssociateEditor,FrontiersinPlantScienceABSTRACTSANDPRESENTATIONSOralPresentations:National/InternationalMeetings

1. MatobaN,BomselM,ArntzenCJandMorTS.“TowardsamucosalvaccineagainstHIV”inCongressonInVitroBiology,Portland,OR,May31–June4,2003

2. MatobaN,MorTSandArntzenCJ.“Heat-stableoralvaccinesfromtransgenicplants”inPharmaceuticalSciencesWorldCongress2004,Kyoto,Japan,May30–June3,2004.(Invitedtalk)

3. MatobaN,MagérusA,GeyerBC,AlfsenA,HansonCV,ArntzenCJ,BomselMandMorTS.“HumoralimmunityinducedbyCTB-MPR649-684forthepreventionofHIV-1mucosaltransmission/infection”inPlant-BasedVaccine&Antibodies,Prague,CzechRepublic,June8–10,2005.

4. BarnettB,ConwayH,HuskA,PickelM,ArntzenC,ZhangP,QuinnanG,MooneyJ,HansonC,TakahashiA,TannoK,TanakaH,andMatobaN.“Developmentofarobustandrapidplant

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expressionsystemforActinohivin,anovelanti-HIV-1proteintargetingtheenvelopehigh-mannosecluster”Plant-madePharmaceuticalsMeeting,Louisville/Owensboro,KY,June2009.

5. HamorskyK,SankaranS,DavisK,PalmerK,MatobaN.“HighlyefficientproductionofrecombinantcholeratoxinBsubunitformassvaccinationagainstcholera”TheGordonConferenceTropicalInfectiousDiseases,March13–18,Galveston,TX.

6. MatobaN,BaldaufK,HuskA,MorrisMK,HamorskyK,PalmerK,HansonC.“ANovel,Non-ARV-BasedTopicalHIV-1MicrobicideCandidateTargetingViralHigh-MannoseGlycans.”2ndAntiviralsCongress,Cambridge,MA,November2012.

7. MatobaN.“BioProductionofRecombinantProteinPharmaceuticalsinPlants”.InvitedKeynoteSpeakeratSymposiumonPlantBiotechIndustryNowandFutureinNiigata.Niigata,Japan,April2013.

8. Husk A*, Kasinger L, Hamorsky K, Matoba N. “Engineering, characterization, and anti-HIV activity of a bispecific HIV entry inhibitor” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

9. Royal J*, Baldauf K, Kouokam J, Matoba N. “Plant-made cholera toxin B subunit as a candidate oral immunotherapeutic agent against ulcerative colitis” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

10. Fuqua J*, Riedel S, Lasnik A, Walker J, Hamorsky K, Matoba N, Palmer K. “Mucosal delivery of a L2-based fusion vaccine provides cross-neutralizing protection against multiple HPV types” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

11. Hamorsky K*, Kouokam J, McMullen J, Nelson B, Bennett L, Husk A, Kajiura H, Fujiyama K, Matoba N. “N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: Impacts on host stress response, production yield and vaccine potential” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

12. Matoba N*. “The PREVENT integrated preclinical development program to develop plant-produced Griffithsin as a topical microbicide” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

13. Matoba N*, Hamorsky K, Husk A, Kouokam C, Grooms T, Morris MK, Hanson C, Hume S, Vausselin T, Dubuisson J. “Plant virus vector-based engineering and production of AvFc, a novel lectin-Fc fusion protein with broad anti-HIV/HCV activity.” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

14. Matoba N*. “Plant viral vectors for efficient bioproduciton of protein pharmaceuticals in plants” The 27th International Conference on Arabidopsis Research, Gyeongju, South Korea, June 29 – July 3, 2016. Invited Talk, Session Chair (Plant Biotech).

15. Matoba N*. “Rapid Engineering and Production of Novel Biopharmaceuticals Using a Plant Viral Vector System” The 16th Annual PepTalk- Plant-Based Expression and Synthetic Biology, January 10 – 11, 2017, San Diego, CA. Invited Talk.

16. Royal J*, Baldauf K, Yaddanapudi K, Dryden GW, Matoba N. “Epicertin, a cholera toxin B subunit variant, enhances intestinal wound healing in a mouse acute colitis model and human ulcerative colitis colon explants via an unfolded protein response” Digestive Disease Week 2018, Washington, D.C., June 2 – 5, 2018. Mr. Royal received a Basic Science Travel Award.

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OralPresentations:Local/RegionalMeetings

1. YoshikawaM,MatobaN,NakagiriRandSasakiR.“OvokininisanagonistforangiotensinAT2receptors”MeetingofTheJapanesePeptideSociety,Tsukuba,Japan,October1996.

2. MatobaN,NakagiriR,SasakiRandYoshikawaM.“Designofpotentanti-hypertensivepeptidesbasedonovokininandcharacterizationoftheanti-hypertensiveactivity”AnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Tokyo,Japan,April1997.

3. MatobaN,AlfsenA,BomselM,HansonCV,ArntzenCJandMorTS.“DevelopingVaccinesAimedatBlockingHIV-1Transmission”inAnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Kyoto,Japan,April2006

4. MatobaN,MittmanM,GriffinTA,CherniIandMorTS.“ExpressionofamucosalsubunitHIV-1vaccinecandidateintransgenicN.benthamiana”inAnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Tokyo,Japan,March2007.

5. HamorskyK,BaldaufK,MatobaN.“Large-scaleproductionofrecombinantproteinsinplantsenablingmucosaldeliveryofbiotherapeuticsbasedonnanotechnology.KYNanotechSymposium,Louisville,KY,August2013.

PosterPresentations:National/InternationalMeetings

1. MatobaN,BomselM,ArntzenCJandMorTS.“ImmunostimulatoryeffectofaHIVenvelope-derivedpeptide”in11thInternationalCongressofMucosalImmunology,Orlando,FL,June16–20,2002.

2. MatobaN,MagérusA,GeyerBC,AlfsenA,HansonCV,ArntzenCJ,BomselMandMorTS.“HumoralimmunityinducedbyCTB-P1forthepreventionofHIV-1mucosaltransmission/infection”inkeystoneSymposiaX-8HIVVaccines,Banff,Canada,April9–15,2005.

3. MatobaN,MagérusA,GeyerBC,AlfsenA,HansonCV,ArntzenCJ,BomselMandMorTS.“HumoralimmunityinducedbyCTB-MPR649-684towardsthepreventionofHIV-1mucosaltransmission/infection”in12thInternationalCongressofMucosalImmunology,Boston,MA,June24–30,2005.

4. MatobaN,GeyerBC,AlfsenA,ArntzenCJ,BomselMandMorTS.“Humoralimmunitydirectedatgp41-MPRforthepreventionofHIV-1mucosaltransmission”in5thInternationalConference:AIDSVaccine2006,Amsterdam,Netherlands,August29–September1,2006.

5. MatobaN,MittmanM,GriffinTA,CherniI,AlfsenA,BomselM,ArntzenCJandMorTS“AmucosalsubunitHIV-1vaccinecandidateexpressedinN.benthamiana”inNewCellsforNewVaccines,CoralCables,FL,September28–29,2006.

6. MatobaN,MittmanM,GriffinTA,CherniI,ArntzenCJandMorTS.“ExpressionofanHIV-1vaccinecandidateexpressedinN.benthamianaplant”inPlant-BasedVaccine&Antibodies,Verona,Italy,June18–20,2007.

7. MatobaN,GriffinTA,CherniI,MittmanM,DoranJD,AlfsenA,HansonCV,BomselM,andMorTS.“Transcytosis-blockingAbstargetnon-neutralizingepitopesinthemembraneproximal

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regionoftheHIV-1envelopeproteingp41”inKeystoneSymposiaX-7HIVVaccines:ProgressandProspects,Banff,Canada,March26–April1,2008.

8. KessansSA,FraterJ,MatobaN,andMorTS.“PlantexpressionofchimericGag/gp41virus-likeparticlesasamucosally-targetedsubunitvaccineagainstHIV-1”AIDSVaccine2009,Paris,France,October2009

9. MatobaN,CherniI,KessansS,FraterJ,PrestonK,BomselM,andMorTS.“Biochemicalandimmunologicalcharacterizationoftheplant-derivedcandidateHIV-1mucosalvaccineCTB-MPR”AIDSVaccine2009,Paris,France,October2009

10. MatobaN,HuskA,BarnettB,PickelM,ArntzenC,TakahashiA,TannoK,MontefioriD,HansonCandTanakaH.“InitialFeasibilityAnalysisoftheMannoseCluster-specificLectinActinohivinasaCandidateHIV-1Microbicide”TheSecondInternationalConferenceonModernMucosalVaccines,Adjuvants&Microbicides,April28–30,2010,Dublin,Ireland

11. MatobaN,HuskA,BarnettB,ArntzenC,MontefioriD,CaoH,HansonCandTanakaH.“InVitroAnti-HIV-1EfficacyProfileandPlant-basedRecombinantExpressionofActinohivin,anEnvMannoseCluster-specificLectin”2010InternationalMicrobicideConference,May22–25,2010,Pittsburgh,PA–Ireceivedascholarshipawardonthispresentation.

12. HamorskyK,KouokamJC,BennettL,MatobaN.Plant-derivedCholeraToxinBSubunitforAideinMassVaccinationagainstCholera.NewCells,NewVaccineVI,Wilmington,DE,March2012.

13. BaldaufK,KouokamJC,JalaVR,BodduluriH,MatobaN.“APlant-ProducedCholeraToxinBSubunitPreventsAcuteColitisinaMouseModel”2013InternationalCongressofMucosalImmunology,Vancouver,Canada,July2013.

14. HamorskyKT,HuskA,MorrisMK,HansonC,HumeS,DubuissonJ,MatobaN.“Engineering,characterizationandanti-HIV/HCVactivityofAvaren-Fc,anoligomannose-specificlectin-humanIgG1Fcchimera”NextGenerationProteinTherapeuticsSummit,SanFrancisco,CA,June4–6,2014.

15. HamorskyKT,HuskA,KouokamJ,MorrisMK,HansonC,HumeS,Grooms-WilliamsT,VausselinT,DubuissonJ,MatobaN.“AvFc,anoligomannose-specificlectin-humanIgG1Fcchimera,isacandidateantiviralbiotherapeuticagentexhibitingbroadanti-HIV/HCVactivity”ICAAC2014,Washington,DC,September5–9,2014

16. Grooms-WilliamsT,KouokamJC,JensonAB,MatobaN.“ToxicologicalAnalysisofAvaren-Fc:ApotentialHIVmicrobicide”HIVR4P,CapeTown,SouthAfrica,28–31,October2014.

17. HamorskyK,HuskA,MatobaN.“EngineeringandCharacterizationofaBispecificEntryInhibitorfortheNon-VaccineBiomedicalPreventionofHIV-1Infection”HIVR4P,CapeTown,SouthAfrica,28–31,October2014.

18. MatobaN,KouokamC,CaryR,FreelsA,HamorskyK.“ThepotentialofAvFc,anFc-fusedhigh-mannoseglycan-specificlectin,forbroadspectrumanti-cancerimmunotherapy”2015KeystoneSymposiaConferenceJ8:AntibodiesasDrugs:ImmunologicalScaffoldsasTherapeutics,Banff,Canada,February8-13,2015.

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19. Hamorsky KT, Seber L, Husk A, Matoba N*. “Engineering, characterization, and anti-HIV activity of a Bispecific HIV Entry Inhibitor” Keystone Symposium: Antibodies as Drugs, Banff, Canada, February 9 – 12, 2015.

20. MatobaN,HuskA,KouokamC,HamorskyK,Grooms-WilliamsT,MahajanG.“AvFc,anovelFcfusionproteintargetingEnvhigh-mannoseglycans”CROI2015,Seattle,WA,February23-26,2015.

21. Zahin M*, Joh J, Khanal S, Husk A, Mason H, Kim S, Matoba N, Jenson A. “Plant-based recombinant vaccine: purification of HPV16 L1 protein and VLPs from tobacco leaves” Plant-Based Vaccines, Antibodies & Biologics. June 8 – 10, 2015, Lausanne, Switzerland.

22. Matoba N*, Husk A, Kouokam JC, Hamorsky K, Grooms T. “A lectibody targeting Env glycans exhibits broad antiviral activity against HIV virions and infected cells” HIV R4P, Chicago, IL, October 17 – 20, 2016.

23. Hamorsky K*, Moore L, Wang L, Rohan L, Palmer K, Matoba N. “Quality studies to support the biopharmaceutical development of the rectal microbicide candidate Griffithsin” HIV R4P, Chicago, IL, October 17 – 20, 2016.

24. Fuqua J*, Hamorsky K, Wang L, Kramzer L, Matoba N, Rohan L, Palmer K. “Characterization of an oxidation resistant griffithsin for use as an HIV microbicide” HIV R4P, Chicago, IL, October 17 – 20, 2016.

25. RoyalJ*,BaldaufK,YaddanapudiK,DrydenGW,MatobaN.“OralAdministrationofaCholeraVaccineAntigenFacilitatesColonicMucosalHealinginaMurineModelofColitis”DigestiveDiseaseWeek2017,Chicago,IL,May6–9,2017.

26. GuoH*,MatobaN,HamorskyK,RoyalJ.“DevelopmentofPETimagingagentsfortargetinghigh-mannose-typeglycansoncancercells”SocietyofNuclearMedicineandMolecularImagingAnnualMeeting,Denver,CO,June10–14,2017.

27. RoyalJ*,OhYJ,GalandiukS,MatobaN.“Epicertinenhancesintestinalwoundhealinginamousecolitismodelandhumanulcerativecolitiscolonexplants”Crohn’s&ColitisCongress,LasVegas,NV,Jan18–20,2018.

PosterPresentations:Local/RegionalMeetings

1. MatobaN,UsuiH,FujitaHandYoshikawaM.“Mechanismofananti-hypertensiveactivityofovokinin,apeptidederivedfromovalbumin”AnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Nagoya,Japan,April1998.

2. MatobaN,UsuiHandYoshikawaM.“Designofpotentanti-hypertensivepeptidesbasedonobokininIII”AnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Fukuoka,Japan,April1999.

3. MatobaN,DoyamaN,MaruyamaN,UtsumiSandYoshikawaM.“GeneticintroductionofanovokininIIIderivativepeptidepossessingpotentanti-hypertensiveactivityintoaheterologousfoodproteins”AnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Tokyo,Japan,April2000.

4. MatobaN,YamadaY,DoyamaN,MaruyamaN,UtsumiSandYoshikawaM.“Designofahighlypotentanti-hypertensivepeptidederivedfromovokininIIIandgeneticintroductionofthe

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peptideintoasoybeanprotein”AnnualmeetingofJapanSocietyforBioscience,Biotechnology,andAgrochemistry,Kyoto,Japan,March2001.

5. MatobaN,BarnettB,HuskA,andConwayH.“DevelopmentofPlant-basedHIV-1MicrobicidesandVaccinesTargetingtheEnvelopeHigh-mannoseClusters”Research!Louisville,UniversityofLouisville,Louisville,KY,October2009.

6. MatobaN,BarnettB,HuskA,MontefioriD,andTanakaH.“DevelopmentofPlant-basedHIVMicrobicidesandVaccinesbytargetingtheHigh-mannoseClusterontheEnvGlycoproteingp120”JamesGrahamBrownCancerCenter8thAnnualRetreat,UniversityofLouisville,Louisville,KY,November,2009.–IreceivedRogerH.HerzigJuniorFacultyResearchPrize(3rdplace)

7. HuskA,SankaranS,PalmerKEandMatobaN.“HIV-1NeutralizationProfileandPlant-BasedRecombinantExpressionofActinohivinandDerivatives:ComparingPotencyandgp120BindingAffinityforMonomerandDimerMolecules” Research!Louisville,UniversityofLouisville,October2010– Mr.Husk,ResearchTechinmylab,receivedGrandPrizeinProfessionalStaffcategory.

8. HuskA,SankaranS,PalmerKE,MatobaN.“HIV-1NeutralizationProfileandPlant-BasedRecombinantExpressionofActinohivinandDerivatives:ComparingPotencyandgp120BindingAffinityforMonomerandDimerMolecules”JamesGrahamBrownCancerCenter9thAnnualRetreat,UniversityofLouisville,November,2010.

9. HamorskyK,SankaranS,WalkerJ,PalmerK,MatobaN.“EvaluationofPlant-derivedN-mannosylatedCholeraToxinBsubunitsonMucosalImmunogenicity”JamesGrahamBrownCancerCenter9thAnnualRetreat,UniversityofLouisville,November,2010.

10. HumphreyA,HamorskyK,MettsB,SankaranS,BomselM,MatobaN.“Creationofananti-HIVMicrobicideUsingIgA177”2010KentuckyAcademyofScienceAnnualMeeting,heldatWesternKentuckyUniversity,BowlingGreen,KY,November,2010–Ms.Humphrey,anundergraduate

interninmylab,receivedFirstPlaceintheHealthSciencescategory.

11. LasnikA,MatobaN,PalmerK.Investigationoftheimmunogenicityofgriffithsin-basedvaginalmicrobicidesforHIVprevention.Research!LouisvilleOctober2011.

12. HuskA,SankaranS,HamorskyK,PalmerK,MatobaN.“ProteinEngineeringoftheMannose-SpecificLectinActinohivinforEnhancedAntiviralActivityandRecombinantProducibility”JamesGrahamBrownCancerCenterRetreat,October28,Louisville,KY.

13. HamorskyK,KouokamJ,BennettL,DavisK,PalmerK,MatobaN.“InvestigationofImmunomodulatoryActivityofPlant-derivedCholeraToxinB”JamesGrahamBrownCancerCenterRetreat,October28,Louisville,KY

14. BennettL,HamorskyK,MatobaN.“UtilizationofPlant-derivedCholeraToxinBSubunitforMassVaccinationagainstCholera”2011KentuckyAcademyofScienceAnnualMeeting,heldatMurrayUniversity,Murray,KY,November4–5,2011

15. BaldaufK,KouokamJ,HamorskyK,JalaV,BodduluriH,MatobaN.Oraladministrationofplant-madecholeratoxinBsubunitmitigatesinflammationinamousemodelofcolitis.Research!Louisville,Louisville,KY,September2012.

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16. HamorskyK,Grooms-WilliamsT,HuskA,BennettL,PalmerK,MatobaN.“EfficientbioproductionofIgGmonoclonalantibodiesinNicotianaplants.”Research!Louisville,Louisville,KY,September2012.

17. SpeerD,HuskA,MatobaN.“Plant-basedProductionofBi-specificHIVInhibitorasTopicalMicrobicide”2012KentuckyAcademyofScienceUndergraduatePosterPresentation,October19–20,2012,Richmond,KY.–Mr.Speer,anundergraduateinterninmylab,receivedThirdPlaceintheMolecular

andCellularBiologycategory.

18. BaldaufK,KouokamJ,HamorskyK,JalaV,BodduluriH,MatobaN.“Oraladministrationofplant-madecholeratoxinBsubunitmitigatesinflammationinamousemodelofcolitis.”JamesGrahamBrownCancerCenterRetreat,October2012,Louisville,KY.

19. HamorskyK,Grooms-WilliamsT,HuskA,BennettL,PalmerK,MatobaN.“EfficientbioproductionofIgGmonoclonalantibodiesinNicotianaplants.”JamesGrahamBrownCancerCenterRetreat,October2012,Louisville,KY.

20. Grooms-WilliamsT,HamorskyKT,BennettL,HuskA,MatobaN.“SynergisticactivityprofileofthebroadlyHIVneutralizingmonoclonalantibodyVRC01incombinationwithotherHIVinhibitors”JamesGrahamBrownCancerCenterRetreat,October2012,Louisville,KY.

21. HuskA,SpeerD,MatobaN.“Plant-basedProductionofBi-specificHIVInhibitorasTopicalMicrobicide””JamesGrahamBrownCancerCenterRetreat,October2012,Louisville,KY.–Mr.

Husk,SrResearchTechinmylab,receivedSecondPlaceaward.

22. BennettL,HamorskyKT,KouokamJC,BaldaufK,MatobaN.“Aplant-producedcholeratoxinBsubunitvariantdevoidofcrypticN-glycanmodificationretainskeymolecularpropertiesandoralimmunogenicity”JamesGrahamBrownCancerCenterRetreat,October2012,Louisville,KY.

23. BaldaufK,KouokamJC,BodduluriH,MatobaN.“APlant-ProducedCholeraToxinBSubunitPreventsAcuteColitisinaMouseModel”Research!Louisville,Louisville,KY,September2013.

24. HamorskyKT,HuskA,MorrisMK,HansonC,HumeS,DubuissonJ,MatobaN.“Engineering,characterizationandanti-HIV/HCVactivityofAvaren-Fc,anoligomannose-specificlectin-humanIgG1Fcchimera”BrownCancerCenterRetreat,Louisville,KY,October2013

25. HuskA,HamorskyKT,MatobaN.“Plant-basedproductionandcharacterizationofanovelbispecificHIVmicrobicide”BrownCancerCenterRetreat,Louisville,KY,October2013

26. ZahinM,GhimS,JohJ,HumeS,MillerMC,MasonH,MatobaN,JensonAB.“Plantbasedrecombinantvaccine:PurificationofHPV-16L1proteinfromtobaccoleaves”BrownCancerCenterRetreat,Louisville,KY,October2013.

27. KouokamJC,HuskA,BaldaufK,HamorskyKT,MatobaN.“AnOligomannose-specificLectin-FcChimerawithAnti-HIV/HCVActivityExhibitsExcellentinvitroSafetyProfiles”BrownCancerCenterRetreat,Louisville,KY,October2013.

28. Grooms-WilliamsT,HamorskyK,KouokamJ,MorrisMK,HuskA,BaldaufK,HansonC,MatobaN.“ToxicologicalAnalysisofAvaren-Fc:ApotentialHIVvaginalmicrobicide”BrownCancerCenterRetreat,Louisville,KY,October2013.

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29. BaldaufK,KouokamJC,BodduluriH,MatobaN.“APlant-ProducedCholeraToxinBSubunitPreventsAcuteColitisinaMouseModel”BrownCancerCenterRetreat,Louisville,KY,October2013.

30. FuquaJ,AugensteinA,RiedellS,LasnikAB,WalkerJ,HamorskyK,MatobaN,PalmerKE.“Next-generationHPVProphylacticVaccines:OptimizingConstructDesignandEvaluatingMucosalImmunizationRoutes”BrownCancerCenterRetreat,Louisville,KY,October2013.

31. RoyalJM,NelsonBA,BaldaufKJ,KouokamJC,MatobaN.Plant-MadeCholeraToxinBSubunit,anOrallyActiveAnti-inflammatoryProtein,inanAcuteColitisMouseModel:InvestigationofEffectiveDoseandTimeofAdministration”Research!Louisville,Louisville,KY,September2014.

32. BaldaufKJ,KouokamJC,JalaVR,BodduluriH,MatobaN.“OralAdministrationofCholeraToxinBSubunitModulatesGastrointestinalProfileandProtectsAgainstDSS-InducedAcuteColitisinMice”Research!Louisville,Louisville,KY,September2014.

33. Grooms-WilliamsT,KouokamJC,JensonAB,MatobaN.“ToxicologicalAnalysisofAvaren-Fc:ApotentialHIVmicrobicide”Research!Louisville,Louisville,KY,September2014.

34. RoyalJM,HuskAS,HamorskyKT,BennettLJ,MatobaN.“Plant-MadeAnti-TNFαMonoclonalAntibody;anInfliximabBiosimilaragainstIntestinalInflammationandColonCancer.”BrownCancerCenterRetreat,Louisville,KY,October2014.

35. BaldaufKJ,KouokamJC,JalaVR,BodduluriH,MatobaN.“OralAdministrationofCholeraToxinBSubunitModulatesGastrointestinalProfileandProtectsAgainstDSS-InducedAcuteColitisinMice”BrownCancerCenterRetreat,Louisville,KY,October2014.

36. KouokamJC,RoyalL,CaryR,FreelA,HuskA,HamorskyK,MatobaN.“EvaluationoftheHIV-1antiviralAvaren-Fcasapotentialcancerimmunotherapeuticdrug.”BrownCancerCenterRetreat,Louisville,KY,October2014.

37. SeberL,HuskA,HamorskyK,MatobaN.“Plant-basedproductionandcharacterizationofanovelbispecificHIVmicrobicide”BrownCancerCenterRetreat,Louisville,KY,October2014.

38. Grooms-WilliamsT,KouokamJC,JensonAB,MatobaN.“ToxicologicalAnalysisofAvaren-Fc:ApotentialHIVmicrobicide”BrownCancerCenterRetreat,Louisville,KY,October2014.

39. RoyalCL,HuskA,HamorskyK,MatobaN.“InvestigationofthecancerimmunotherapeuticpotentialofAvaren-Fc”KentuckyAcademyofScience100thAnnualMeeting,Lexington,KY,November14–16,2014.Mr.Royal,summerinternstudent,won1stplaceintheHealthSciencecategory.

40. Baldauf K*, Royal J, Kouokam J, Dryden G, Matoba N. “Cholera Toxin B Subunit Protects against Colitis-Associated Colon Cancer in a Mouse Model” Ohio Valley Society of Toxicology 2015 Student/Postdoc Summer Meeting. June 29, 2015, Cincinnati, OH.

41. Freels A*, Cary R, Husk A, Matoba N. “The potential of AvFc, a novel plant-produced lectin-Fc fusion molecule, for use in anticancer immunotherapy” Research!Louisville. October 28, 2015, Louisville, KY.

42. Lasnik A*, Matoba N, Palmer K. “Detection of the antiviral lectin in animal and human plasma” Research!Louisville. October 28, 2015, Louisville, KY.

43. Baldauf K, Royal J*, Kouokam J, Dryden G, Matoba N. “Cholera toxin B subunit protects

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against colitis-associated colon cancer in a mouse model” Research!Louisville. October 28, 2015, Louisville, KY.

44. Zahin M*, Joh J, Khanal S, Husk A, Mason H, Kim S, Matoba N, Jenson A. “Plant-based recombinant vaccine: purification of HPV16 L1 protein and VLPs from tobacco leaves” Research!Louisville. October 28, 2015, Louisville, KY.

45. Loganathan N*, Ryoal JM, Matoba N. “Plant-Made Cholera Toxin B Subunit: A Molecular Look at Wound Healing Mechanisms.” Research!Louisville. October 2016, Louisville, KY. Ms. Loganathan

received a JGBCC High School Scholars Award (1st place)

46. Hamorsky KT*, Kouokam C, Grooms T, Husk A, Freels A, Guo H, Matoba N. A Lectibody Targeting HIV and Cancer-associated High-mannose Glycans” Research!Louisville. October 2016, Louisville, KY.

47. Royal J*, Baldauf K, Matoba N. “Plant-Made Cholera Toxin B Subunit: a Candidate Oral Immunotherapeutic Agent Enhances Colonic Mucosal Wound Healing.” Research!Louisville. October 2016, Louisville, KY. Mr. Royal received a Master’s Basic Science Graduate Student Award (2nd place)

48. Royal J, Oh Y, Galandiuk S, Matoba N. “Epicertin, A Cholera Toxin B Subunit Variant, Enhances Intestinal Wound Healing in a mouse acute colitis model and human ulcerative colitis colon explants” Research!Louisville, University of Louisville, Sep 12, 2017. Mr. Royal received Master’s

Basic Science Graduate Student Award 1st place

49. Dent M, Guo H, Freels A, Telang S, Matoba N. “A Plant-Produced “Lectibody” Selectively Targets High-Mannose Glycans Overexpressed on the Surface of Cancer Cells: Implications for Novel Cancer Therapeutics/Diagnostics Development” Research!Louisville, University of Louisville, Sep 12, 2017.

PUBLICATIONS

Peer-ReviewedPublications(*corresponding/seniorauthor)

1. MatobaN,UsuiH,FujitaH,YoshikawaM.(1999)Anovelanti-hypertensivepeptidederivedfromovalbumininducesnitricoxide-mediatedvasorelaxationinanisolatedSHRmesentericartery.FEBSLett.452:181-184.(PMID:10386586)

2. YoshikawaM,FujitaH,MatobaN,TakenakaY,YamamotoT,YamauchiR,TsurukiT,TakahataK.(2000)Bioactivepeptidesderivedfromfoodproteinspreventinglifestyle-relateddiseases.BioFactors12:143-146.(PMID:11216476)

3. MatobaN,YamadaY,UsuiH,NakagiriR,YoshikawaM.(2001)Designingpotentderivativesofovokinin(2-7),ananti-hypertensivepeptidederivedfromovalbumin.BiosciBiotechnolBiochem.65:736-739.(PMID:11330703)

4. MatobaN,DoyamaN,YamadaY,MaruyamaN,UtsumiS,YoshikawaM.(2001)Designandproductionofgeneticallymodifiedsoybeanproteinwithanti-hypertensiveactivitybyincorporatingpotentanalogueofovokinin(2-7).FEBSLett.497:50-54.(PMID:11376661)

5. YamadaY,MatobaN,UsuiH,OnishiK,YoshikawaM.(2002).Designofpotentanti-hypertensivepeptidebasedonovokinin(2-7).BiosciBiotechnolBiochem.66:1213-1217.(PMID:12162540)

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6. MatobaN,YamadaY,YoshikawaM.(2003)Designofageneticallymodifiedsoybeanproteinpreventinghypertensionbasedonananti-hypertensivepeptidederivedfromovalbumin.CurrMedChem-Cardiovasc&HematolAgents1:197-202.(PMID:15320699)

7. OnishiK,MatobaN,YamadaY,DoyamaN,MaruyamaN,UtsumiS,YoshikawaM.(2004)Optimaldesigningofbeta-conglycinintogeneticallyincorporateRPLKPW,apotentanti-hypertensivepeptide.PEPTIDES25:37-43.(PMID:15003354)

8. MatobaN,MagérusA,GeyerBC,ZhangY,MuralidharanM,AlfsenA,ArntzenCJ,BomselM,MorTS.(2004)AmucosallytargetedsubunitvaccinecandidateelicitingHIV-1transcytosis-blockingAbs.ProcNatlAcadSciUSA.101:13584-13589.(PMID:15347807)

9. MatobaN.(2005)Plant-derivedvaccines:anemergingtechnologytowardsthedistributionofvaccinesindevelopingcountries.Seibutsu-kogakuKaishi83(1):36.InJapanese.

10. MatobaN,ArntzenCJ.(2005)AcurrentperspectiveonmolecularfarmingintheUSA.Invitedreviewfrom:Seibutsu-kogakuKaishi83(11):519-521.InJapanese.

11. MatobaN,GeyerBC,KilbourneJ,AlfsenA,BomselM,MorTS.(2006)Humoralimmuneresponsesbyprime-boostheterologousrouteimmunizationswithCTB-MPR649-684,amucosalsubunitHIV/AIDSvaccinecandidate.Vaccine24:5047-5055.(PMID:16621185)

12. MatobaN,GriffinTA,MittmanM,DoranJD,AlfsenA,MontefioriDC,HansonCV,BomselM,MorTS.(2008)Transcytosis-blockingAbselicitedbyanoligomericimmunogenbasedonthemembraneproximalregionofHIV-1gp41targetnon-neutralizingepitopes.CurrHIVRes3:218-229.(PMID:18473785)

13. MatobaN,KajiuraH,CherniI,DoranJD,BomselM,FujiyamaK,MorTS.(2009)Biochemicalandimmunologicalcharacterizationoftheplant-derivedcandidatehumanimmunodeficiencyvirustype1mucosalvaccineCTB-MPR649-684.PlantBiotechnolJ.7(2):129-145.(PMID:19037902)

14. MatobaN*,HuskAS,BarnettBW,PickelMM,ArntzenCJ,TakahashiA,TannoK,OmuraS,MontefioriDC,MooneyJP,CaoH,HansonCV,TanakaH.(2010)HIV-1NeutralizationProfileandPlant-basedRecombinantExpressionofActinohivin,anEnvGlycan-specificLectinDevoidofT-cellMitogenicActivity.PLoSONE5(6):e11143.(PMID:20559567)

15. MatobaN*,DavisKR,PalmerKE.(2011)RecombinantProteinExpressioninNicotiana.MethodsMolBiol.701:199-219.(PMID:21181532)

16. MatobaN*,ShahN,MorTS*.(2011)HumoralImmunogenicityofanHIV-1EnvelopeResidue649-684Membrane-proximalRegionPeptideFusedtothePlagueAntigenF1-V.Vaccine29(34):5584-5590.(PMID:21693158)

17. KouokamJC,HuskensD,ScholsD,JohannemannA,RiedellSK,WalterW,WalkerJM,MatobaN,O’KeefeBR,PalmerKE.(2011)InvestigationofGriffithsin’sInteractionswithHumanCellsConfirmsItsOutstandingSafetyandEfficacyProfileasaMicrobicideCandidate.PLoSONE6(8):e22635.(PMID:21829638)

18. MatsudaR,TaharaA,MatobaN,FujiwaraK.(2012)VirusVector-MediatedRapidProteinProductioninNicotianabenthamiana:EffectsofTemperatureandPhotosyntheticPhotonFluxDensityonHemagglutininAccumulation.EnvironmentControlinBiology.50(4):375-381.

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19. HamorskyKT,KouokamJC,BennettLJ,BaldaufKJ,KajiuraH,FujiyamaK,MatobaN*.(2013)RapidandScalablePlant-basedProductionofaCholeraToxinBSubunitVarianttoAidinMassVaccinationagainstCholeraOutbreaks.PLoSNeglTropDis7(3):2046.(PMID:23505583)

20. HamorskyKT,Grooms-WilliamsT,HuskAS,BennettLJ,PalmerKE,MatobaN*.(2013)Efficientsingletobamoviralvector-basedbioproductionofbroadlyneutralizinganti-HIV-1monoclonalantibodyVRC01inNicotianaplantsanditsutilityincombinationmicrobicides.AntimicrobAgentsChemother.57(5):2076-86.(PMID:23403432)

21. KessansSA,LinhartMD,MatobaN*,MorTS*.(2013)BiologicalandbiochemicalcharacterizationofHIV-1Gag/dgp41virus-likeparticlesexpressedinNicotianabenthamiana.PlantBiotechnolJ.11(6)681-90.(PMID:23506331)

22. FujiuchiN,MatsudaR,MatobaN,FujiwaraK.(2014)EffectofnitrateconcentrationinnutrientsolutiononhemagglutinincontentofNicotianabenthamianaleavesinaviralvector-mediatedtransientgeneexpressionsystem.PlantBiotechnol31(3): 207-211.

23. LeeH,CherniI,YuH,FrommeR,DoranJD,GrotjohannI,BasuS,DornerK,AquilaA,BartyA,BoutetS,ChapmanHN,DoakRB,HogueBG,HunterMS,JamesDR,KirianRA,KupitzC,LawrenceRM,LiuH,NassK,SchlichtingI,SchmidtKE,SeibertMM,ShoemanRL,SpenceJ,StellatoF,WeierstallU,WilliamsGJ,YoonC,WangD,ZatsepinNA,MatobaN,FrommeP,MorTS.(2014)Expression,purificationandcrystallizationofCTB-MPR,acandidatemucosalvaccinecomponentagainstHIV-1.IUCrJ1:305-317.(PMID:25295172)

24. HuskA,HamorskyK,MatobaN*.(2014)MonoclonalAntibodyPurification(NicotianabenthamianaPlants).BioProtoc.20;4(2).pii:e1034.(PMID:27547788)

25. HamorskyKT,KouokamJC,McMullenJ,NelsonB,BennettL,HuskA,KajiuraH,FujiyamaK,MatobaN*.(2015)N-GlycosylationofcholeratoxinBsubunitinNicotianabenthamiana:impactsonhoststressresponse,productionyieldandvaccinepotential.SciRep5:8003.(PMID:25614217)

26. BaldaufKJ,RoyalJM,HamorskyKT,MatobaN*.(2015)CholeratoxinB:Onesubunitwithmanypharmaceuticalapplications.Toxins7:974-996.(PMID:25802972)

27. FuquaJL,HamorskyK,KhalsaG,MatobaN,PalmerKE.(2015)Bulkproductionoftheantivirallectingriffithsin.PlantBiotechnolJ13(8):1160-8.(PMID:26176205)

28. MatobaN*.(2015)N-GlycosylationofcholeratoxinBsubunit:serendipityfornovelplant-madevaccines?FrontPlantSci6:1132.(PMID:26732492)

29. FujiuchiN,MatsudaR,MatobaN,FujiwaraK.(2016)RemovalofBacterialSuspensionWaterOccupyingtheIntercellularSpaceofDetachedLeavesafterAgroinfiltrationImprovestheYieldofRecombinantHemagglutinininaNicotianabenthamianaTransientGeneExpressionSystem.BiotechnolBioeng113(4):901-6.(PMID:26461274)

30. FujiuchiN,MatobaN,MatsudaR(2016)EnvironmentControltoimproverecombinantproteinyieldsinplantsbasedonAgrobacterium-mediatedtransientgeneexpression.FrontBioengBiotechnol4:23.doi:10.3389/fbioe.2016.00023.(PMID:27014686)

31. KessansSA,LinhartM,MeadorLR,KilbourneJ,FrommeP,MatobaN,MorTS.(2016)

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Immunologicalcharacterizationofplant-basedHIV-1gag/dgp41virus-likeparticles.PLoSONE11(3):e0151842.(PMID:26986483)

32. ZahinM,JohJoongho,KhanalS,HuskA,MasonH,WarzechaH,GhimShin-je,MillerD,MatobaN,JensonA(2016)Plant-basedrecombinantvaccine:PurificationofHPV16L1proteinandVLPsfromtobaccoleaves.PLoSONE11(8):e0160995.(PMID:27518899)

33. GroomsTN,VuongHR,TyoKM,MalikDA,SimsLB,WhittingtonCP,PalmerKE,MatobaN*,Steinback-RankinsJM*.(2016)Griffithsin-ModifiedElectrospunFibersasaDeliveryScaffoldtoPreventHIVInfection.AntimicrobAgentsChemother60:6518-6531.*co-senior/correspondingauthors(PMID:27550363)

34. BaldaufKJ,RoyalJM,KouokamJC,HaribabuB,JalaVR,YaddanapudiK,HamorskyKT,DrydenGW,MatobaN*.(2017)OraladministrationofarecombinantcholeratoxinBsubunitpromotesmucosalhealinginthecolon.MucosalImmunol10:887-900.(PMID:27805617)

35. FujiuchiN,MatsudaR,MatobaN,FujiwaraK.(2017)EffectsofplantdensityonrecombinanthemagglutininyieldsinanAgrobacterium-mediatedtransientgeneexpressionsystemusingNicotianabenthamianaplants.BiotechnolBioeng114(8):1762-1770.(PMID:28369753)

36. MatsudaR,AbeT,FujiuchiN,MatobaN,FujiwaraK.(2017)EffectoftemperaturepostviralvectorinoculationontheamountofhemagglutinintransientlyexpressedinNicotianabenthamianaleaves.JBioschiBioeng124(3):346-350.(PMID:28460871)

37. HughesMG,TuckerWW,ReddyS,BrierME,KochD,McClainCJ,JonssonCB,MatobaN,ChungD.(2017)RateofHepatitisCViralUptakebyHumanLiversinHumanPatients:LiverTransplantationModelingPrimaryInfectionandImplicationsforStudyingEntryInhibition.PLoSONE12(7):e0180719.(PMID:28732019)

38. RoyalJM,MatobaN*.(2017)TherapeuticpotentialofCholeraToxinBSubunitforthetreatmentofinflammatorydiseasesofthemucosa.Toxins.9(12).pii:E379.(PMID:29168738)

39. RoychowdhuryS,OhYJ,KajiuraH,HamorskyKT,FujiyamaK,MatobaN*.(2018)HydroponictreatmentofNicotianabenthamianawithkifunensinemodifiestheN-glycansofrecombinantglycoproteinantigenstopredominantlyMan9high-mannosetypeupontransientoverexpression.FrontPlantSci9:62.doi:10.3389/fpls.2018.00062.(PMID: 29441088)

40. GirardL,BirseK,HolmJB,GajerP,HumphrysMS,GarberD,GuenthnerP,Noël-RomasL,AbouM,McCorristerS,WestmacottG,WangL,RohanLC,MatobaN,McNichollJ,PalmerKE,RavelJ,BurgenerA.(2018)Impactofthegriffithsinanti-HIVmicrobicideandplacebogelsontherectalmucosalproteomeandmicrobiomeinnon-humanprimates.SciRep8:8059.

Non-peerreviewedarticles,Bookchapters,Proceedings,PamphletorBulletin

1. YoshikawaM,MatobaN,NakagiriR,FujitaHandSasakiR.(1997)OvokininisanagonistforangiotensinAT2receptors.InPeptideChemistry1996TheJapanesePeptideSociety,pp.141-144.

2. OnishiK,MatobaN,YamadaY,UtsumiS,MaruyamaNandYoshikawaM.(2003)Designofhighlyfunctionalsoybeanproteinbygeneticintroductionofanti-hypertensivepeptide.InPeptideScience2002(Ed.byT.Yamada),TheJapanesePeptideSociety,pp.123-126.

3. MatobaNandMorTS.(2006)Plant-derivedsubunitvaccines.In:PlantGeneticEngineeringvol.8:MetabolicEngineering&Molecularpharming–II”Jaiwal,P.K.andSingh,R.P.(Eds.),HaworthPressInc.,NewYork,pp143-183.

4. ShahN,MatobaN,ChangH,HuJ,MorTS.(2008)Plagueantigenfusionswithgp41membraneproximalregionasHIVvaccinecandidate.InVitroCellDevBiolAnim.44:Supplement,S72-73.

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5. MatobaN,KajiuraH,CherniI,DoranJD,BomselM,FujiyamaK,MorTS.(2008)InplantaexpressionandmolecularcharacterizationofthecandidateHIV-1mucosalvaccineCTB-MPR649-684.InVitroCellDevBiolAnim.44:Supplement,S35.

6. KessansS,MatobaN,MorTS.(2008)PlantexpressionofchimericGag/gp41virus-likeparticlesasasubunitvaccineagainstHIV-1.InVitroCellDevBiolAnim.44:Supplement,S72.

7. CherniI,VassallA,MatobaN,MorTS.(2008) MPR649-684-HepBcoreantigenfusionforrnsvirus-likeparticlesinplantsandisimmunogenicinmice.InVitroCellDevBiolAnim.44:Supplement,S57.

8. CherniI,VassallA,KessansS,MatobaN,MorTS.(2008)ChimericPlant-ExpressedHIV-1gp41MembraneProximalRegionDisplayedonHepatitisBCapsidVirus-LikeParticlesisImmunogenicinMice.AIDSResHumRetroviruses24,p39.

9. KessansSA,FraterJ,MatobaN,andMorTS.(2009)PlantexpressionofchimericGag/gp41virus-likeparticlesasamucosally-targetedsubunitvaccineagainstHIV-1.Retrovirology6:Supplement3,p15.

10. MatobaN,CherniI,KessansS,FraterJ,PrestonK,BomselM,MorTS.(2009)Biochemicalandimmunologicalcharacterizationoftheplant-derivedcandidateHIV-1mucosalvaccineCTB-MPR.Retrovirology6:Supplement3,p182.

11. MatobaN*.(2012)VaccineDevelopmentandProductioninPlants.In:TheBasicsofFoodImmunology,YoshikawaandKaminogawa,Eds.Invitedbookchapter.ArticleinJapanese.

12. MatobaN,MatsudaR.(2014)RapidproductionofmonoclonalantibodytherapeuticsandvaccinesinNicotianabenthamiana“plantfactories”.Bioscience&Industry72(2)102-108.Invitedreviewarticle(inJapanese).

13. Grooms-Williams T, Joseph K, Alfred J, Matoba N. (2014) Toxicological Analysis of Avaren-Fc: A Potential HIV Microbicide. AIDS Res Hum Retroviruses. 30(S1): A155-A156.

14. Hamorsky K, Husk A, Matoba N. (2014) Engineering and Characterization of a Bispecific Entry Inhibitor for Non-vaccine Biomedical Prevention of HIV-1 Infection. AIDS Res Hum Retroviruses. 30(S1): A263-A263.

15. MasonHS,ThuenemannE,KiyonoH,KessansS,MatobaN,MorTS.(2015)Chapter65–MucosalVaccinesfromPlantBiotechnology.In:MucosalImmunology(FourthEdition).MesteckyJ,StroberW,RussellMW,CheroutreH,LambrechtBN,KelsallBL.(Eds).Vol2,pp.1271–1289.

16. MooreL,HamorskyKT*,MatobaN*.(2016)ProductionofrecombinantcholeratoxinBsubunitinNicotianabenthamianausingGENEWARE®tobaccomosaicvirusvector.MethodsMolBiol1385:129-37.(PMID:26614286)

17. HamorskyKT*,MatobaN*.(2016)FacilemethodfortheproductionofrecombinantcholeratoxinBsubunitinE.coli.MethodsMolBiol1404:511-8.(PMID:27076319)

18. RoyalJM,BaldaufKJ,YaddanapudiK,DrydenGW,MatobaN*.(2017)Oraladministrationofacholeravaccineantigenfacilitatescolonicmucosalhealinginamurinemodelofcolitis.Gastroenterology152(5):S572-S573.

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19. MatobaN*.(2017).Developmentofbiopharmaceuticalproteinsusingplantvirusvectors.AgriculturalBiotechnology1(7):729-733.Invitedreviewarticle(inJapanese).